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  1. Article: Recombinant Endostatin as a Potential Radiosensitizer in the Treatment of Non-Small Cell Lung Cancer.

    Cunningham, Charnay / Bolcaen, Julie / Bisio, Alessandra / Genis, Amanda / Strijdom, Hans / Vandevoorde, Charlot

    Pharmaceuticals (Basel, Switzerland)

    2023  Volume 16, Issue 2

    Abstract: Non-small cell lung cancer (NSCLC) is the most prevalent type of lung cancer, which is the leading cause of cancer-related deaths worldwide. Over the past decades, tumour angiogenesis has been intensely studied in the treatment of NSCLC due to its ... ...

    Abstract Non-small cell lung cancer (NSCLC) is the most prevalent type of lung cancer, which is the leading cause of cancer-related deaths worldwide. Over the past decades, tumour angiogenesis has been intensely studied in the treatment of NSCLC due to its fundamental role in cancer progression. Several anti-angiogenic drugs, such as recombinant endostatin (RE), have been evaluated in several preclinical and clinical trials, with mixed and often disappointing results. However, there is currently an emerging interest in RE due to its ability to create a vascular normalization window, which could further improve treatment efficacy of the standard NSCLC treatment. This review provides an overview of preclinical and clinical studies that combined RE and radiotherapy for NSCLC treatment. Furthermore, it highlights the ongoing challenges that have to be overcome in order to maximize the benefit; as well as the potential advantage of combinations with particle therapy and immunotherapy, which are rapidly gaining momentum in the treatment landscape of NSCLC. Different angiogenic and immunosuppressive effects are observed between particle therapy and conventional X-ray radiotherapy. The combination of RE, particle therapy and immunotherapy presents a promising future therapeutic triad for NSCLC.
    Language English
    Publishing date 2023-01-31
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph16020219
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Endothelial dysfunction: are we ready to heed the vasculature's early-warning signal?

    Strijdom, Hans

    Cardiovascular journal of Africa

    2012  Volume 23, Issue 4, Page(s) 184–185

    MeSH term(s) Atherosclerosis ; Endothelium, Vascular/physiopathology ; Humans ; Nitric Oxide/metabolism ; Oxidative Stress
    Chemical Substances Nitric Oxide (31C4KY9ESH)
    Language English
    Publishing date 2012-05-22
    Publishing country South Africa
    Document type Editorial ; Comment
    ZDB-ID 2383233-2
    ISSN 1680-0745 ; 1996-3467 ; 1015-9657 ; 1995-1892
    ISSN (online) 1680-0745 ; 1996-3467
    ISSN 1015-9657 ; 1995-1892
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Rooibos (

    Smit-Van Schalkwyk, Michelle / Windvogel, Shantal / Strijdom, Hans

    Cardiovascular journal of Africa

    2020  Volume 31, Issue 4, Page(s) 81–90

    Abstract: Background: Rooibos (: Methods: Vascular function, antioxidant enzyme activity and lipid peroxidation were assessed in male adult rats treated with nicotine (5 mg/kg body weight/day) and 2% RF, 2% RUF or 4% Mel co-administration. Nitric oxide (NO) ... ...

    Abstract Background: Rooibos (
    Methods: Vascular function, antioxidant enzyme activity and lipid peroxidation were assessed in male adult rats treated with nicotine (5 mg/kg body weight/day) and 2% RF, 2% RUF or 4% Mel co-administration. Nitric oxide (NO) production and cell viability were measured in nicotine-exposed rat aortic endothelial cells (AECs) pre-treated with RF (0.015 mg/ml).
    Results: Vascular studies showed that co-administration with RF or Mel exerted anti-contractile and pro-relaxation responses in aortic rings, and increased hepatic superoxide dismutase and catalase activity in nicotine-exposed animals. Co-treatment with Mel additionally decreased lipid peroxidation in nicotine-exposed rats. RUF exerted anti-contractile responses in aortic rings of nicotine-treated animals, while in nicotine-exposed AECs, RF pre-treatment increased intracellular NO levels.
    Conclusions: For the first time, we have shown that rooibos co-treatment exerted beneficial vascular effects in nicotine-exposed rats, and that this was associated with increased antioxidant enzyme activity.
    MeSH term(s) Animals ; Antioxidants/isolation & purification ; Antioxidants/pharmacology ; Aorta/drug effects ; Aorta/metabolism ; Aorta/physiopathology ; Aortic Diseases/chemically induced ; Aortic Diseases/metabolism ; Aortic Diseases/physiopathology ; Aortic Diseases/prevention & control ; Aspalathus/chemistry ; Catalase/metabolism ; Disease Models, Animal ; Lipid Peroxidation/drug effects ; Liver/drug effects ; Liver/metabolism ; Male ; Melatonin/pharmacology ; Nicotine ; Nitric Oxide/metabolism ; Oxidative Stress/drug effects ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Rats, Wistar ; Superoxide Dismutase/metabolism ; Vasodilation/drug effects
    Chemical Substances Antioxidants ; Plant Extracts ; Nitric Oxide (31C4KY9ESH) ; Nicotine (6M3C89ZY6R) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1) ; Melatonin (JL5DK93RCL)
    Language English
    Publishing date 2020-02-07
    Publishing country South Africa
    Document type Comparative Study ; Journal Article
    ZDB-ID 2383233-2
    ISSN 1680-0745 ; 1996-3467 ; 1015-9657 ; 1995-1892
    ISSN (online) 1680-0745 ; 1996-3467
    ISSN 1015-9657 ; 1995-1892
    DOI 10.5830/CVJA-2019-052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: People living with HIV display increased anti-apolipoprotein A1 auto-antibodies, inflammation, and kynurenine metabolites: a case-control study.

    Frias, Miguel A / Pagano, Sabrina / Bararpour, Nasim / Sidibé, Jonathan / Kamau, Festus / Fétaud-Lapierre, Vanessa / Hudson, Peter / Thomas, Aurélien / Lecour, Sandrine / Strijdom, Hans / Vuilleumier, Nicolas

    Frontiers in cardiovascular medicine

    2024  Volume 11, Page(s) 1343361

    Abstract: Objective: This study aimed to study the relationship between auto-antibodies against apolipoprotein A1 (anti-apoA1 IgG), human immunodeficiency virus (HIV) infection, anti-retroviral therapy (ART), and the tryptophan pathways in HIV-related ... ...

    Abstract Objective: This study aimed to study the relationship between auto-antibodies against apolipoprotein A1 (anti-apoA1 IgG), human immunodeficiency virus (HIV) infection, anti-retroviral therapy (ART), and the tryptophan pathways in HIV-related cardiovascular disease.
    Design: This case-control study conducted in South Africa consisted of control volunteers (
    Methods: Anti-apoA1 IgG levels were assessed by an in-house ELISA. Inflammatory biomarkers were measured with the Meso Scale Discovery® platform, and kynurenine pathway metabolites were assessed using targeted metabolomic profiling conducted by liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS).
    Results: Cardiovascular risk scores and vascular measures exhibited similarities across the three groups, while important differences were observed in systemic inflammatory and tryptophan pathways. Anti-apoA1 IgG seropositivity rates were 15%, 40%, and 70% in control volunteers, PLWH ART-treated, and PLWH ART-naïve, respectively. Circulating anti-apoA1 IgG levels were significantly negatively associated with CD4+ cell counts and positively associated with viremia and pro-inflammatory biomarkers (IFNγ, TNFα, MIPα, ICAM-1, VCAM-1). While circulating anti-apoA1 IgG levels were associated with increased levels of kynurenine in both control volunteers and PLWH, the kynurenine/tryptophan ratio was significantly increased in PLWH ART-treated.
    Conclusion: HIV infection increases the humoral response against apoA1, which is associated with established HIV severity criteria and kynurenine pathway activation.
    Language English
    Publishing date 2024-02-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2024.1343361
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Mobility Deviations in Adults With Human Immunodeficiency Virus: A Cross-Sectional Assessment Using Gait Analysis, Functional Performance, and Self-Report.

    Berner, Karina / Gouelle, Arnaud / Strijdom, Hans / Essop, M Faadiel / Webster, Ingrid / Louw, Quinette

    Open forum infectious diseases

    2021  Volume 8, Issue 9, Page(s) ofab425

    Abstract: Background: Little is known about how human immunodeficiency virus (HIV) affects walking biomechanics, or about associations between HIV-related gait deviations, functional performance, and self-reported outcomes. This paper reports on (1) gait ... ...

    Abstract Background: Little is known about how human immunodeficiency virus (HIV) affects walking biomechanics, or about associations between HIV-related gait deviations, functional performance, and self-reported outcomes. This paper reports on (1) gait biomechanics and variability in people with HIV (PWH) and (2) associations with clinical tests, self-reported function, and falls.
    Methods: A cross-sectional study tested consecutively sampled PWH (n = 50) and HIV-seronegative participants ([SNP] n = 50). Participants underwent 3-dimensional gait analysis, performed clinical tests (short walk and single leg stance tests with and without dual tasking, chair-rise tests, and a physical performance battery), and completed questionnaires about function and falls. Between-group comparisons were done using analysis of covariance. Linear correlations between gait variability, clinical tests, and patient-reported outcomes were established.
    Results: People with HIV and SNP had comparable median ages (PWH = 36.6, interquartile range [IQR] = 32.0-45.6]; SNP = 31.1, IQR = 23.2-45.1). Compared with SNP, PWH walked slower (adjusted mean difference [MD] = -0.2 meters per second [m/s], 95% confidence interval [CI] = -0.3 to -0.1) with greater variability (adjusted MD = 14.7 variability score points, 95% CI = 9.9-19.5). Moreover, PWH were slower in five-times sit-to-stand (5STS) performance (adjusted MD = 1.9 seconds, 95% CI = 1.00-2.9). Significant deviations in hip kinematics (increased flexion; adjusted MDs = 2.4°-2.8°,
    Conclusions: People with HIV presented with biomechanical deviations suggestive of a slowed and variable gait, especially under cognitive challenges. Five-times STS may be useful to screen for gait deviations in PWH.
    Language English
    Publishing date 2021-08-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2757767-3
    ISSN 2328-8957
    ISSN 2328-8957
    DOI 10.1093/ofid/ofab425
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Concentrations of Efavirenz, Tenofovir, and Emtricitabine in Obesity: A Cross-Sectional Study.

    Solanke, Toyosi / Kamau, Festus / Esterhuizen, Tonya / Maartens, Gary / Khoo, Saye / Joska, John A / Kellermann, Tracy / Strijdom, Hans / Decloedt, Eric H

    Journal of acquired immune deficiency syndromes (1999)

    2022  Volume 91, Issue 1, Page(s) 101–108

    Abstract: Background: Obesity is increasing worldwide including in people living with HIV (PLWH). Antiretroviral pharmacokinetic data in obesity are limited.: Objectives: To measure antiretroviral drug concentrations in obese and nonobese PLWH treated with the ...

    Abstract Background: Obesity is increasing worldwide including in people living with HIV (PLWH). Antiretroviral pharmacokinetic data in obesity are limited.
    Objectives: To measure antiretroviral drug concentrations in obese and nonobese PLWH treated with the fixed-dose combination of efavirenz-tenofovir-emtricitabine. To determine pharmacokinetic differences across indicators of obesity and their associated immunovirological outcomes.
    Methods: We conducted a cross-sectional sample analysis of 2 cohort studies. We measured mid-dose efavirenz, 8-hydroxy-efavirenz, tenofovir, and emtricitabine concentrations. Antiretroviral drug concentrations were analyzed by body mass index (BMI), waist circumference (WC), and waist-to-hip ratio (WHR).
    Results: We performed a study of 213 participants: General obesity was detected in 20.4% using BMI and abdominal obesity in 53.6% using WC and 62.4% using WHR, respectively. The median concentrations of all antiretroviral drugs were lower among obese participants determined by BMI and WC, with efavirenz showing greater differences than tenofovir or emtricitabine. For BMI, results were most striking for efavirenz (1752.3 vs 2342.9 ng/mL, P = 0.002) with lower concentrations in obese participants. Using WC, efavirenz (1845.8 vs 2571.2 ng/mL, P < 0.001), tenofovir (65.8 vs 73.2 ng/mL, P = 0.036), and emtricitabine (159.5 vs 221.0 ng/mL, P = 0.005) concentrations were lower in obese participants. Eight-hydroxyefavirenz concentrations were similar in nonobese and obese participants for WC. Using WHR, the concentrations of all antiretroviral drugs were lower in the obese population, most strikingly for emtricitabine (173.5 vs 229.0 ng/mL, P = 0.015). There were no immunovirological associations.
    Conclusion: We found lower antiretroviral concentrations in all obese groups, most strikingly in participants with abdominal obesity determined by WC. Lower drug concentrations had no immunovirological associations.
    MeSH term(s) Adenine/pharmacokinetics ; Adenine/therapeutic use ; Alkynes ; Anti-Retroviral Agents/therapeutic use ; Benzoxazines/therapeutic use ; Cross-Sectional Studies ; Cyclopropanes ; Emtricitabine/therapeutic use ; HIV Infections/drug therapy ; Humans ; Obesity, Abdominal/drug therapy ; Tenofovir/therapeutic use
    Chemical Substances Alkynes ; Anti-Retroviral Agents ; Benzoxazines ; Cyclopropanes ; Tenofovir (99YXE507IL) ; Emtricitabine (G70B4ETF4S) ; Adenine (JAC85A2161) ; efavirenz (JE6H2O27P8)
    Language English
    Publishing date 2022-08-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 645053-2
    ISSN 1944-7884 ; 1077-9450 ; 0897-5965 ; 0894-9255 ; 1525-4135
    ISSN (online) 1944-7884 ; 1077-9450
    ISSN 0897-5965 ; 0894-9255 ; 1525-4135
    DOI 10.1097/QAI.0000000000003025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: HIV-related cardiovascular disease: any role for high-density lipoproteins?

    Hudson, Peter / Woudberg, Nicholas J / Kamau, Festus / Strijdom, Hans / Frias, Miguel A / Lecour, Sandrine

    American journal of physiology. Heart and circulatory physiology

    2020  Volume 319, Issue 6, Page(s) H1221–H1226

    Abstract: The introduction of antiretroviral therapy (ART) has improved the life expectancy of patients infected with human immunodeficiency virus (HIV). However, this population is at an increased risk for noncommunicable diseases, including atherosclerotic ... ...

    Abstract The introduction of antiretroviral therapy (ART) has improved the life expectancy of patients infected with human immunodeficiency virus (HIV). However, this population is at an increased risk for noncommunicable diseases, including atherosclerotic cardiovascular disease (CVD). Both ART and viral infection may be potential contributors to the pathophysiology of HIV-related CVD. The mechanisms behind this remain unclear, but it is critical to delineate early biomarkers of cardiovascular risk in the HIV population. In this review, we postulate that potential biomarkers could include alterations to high-density lipoprotein (HDL). Indeed, recent data suggest that HIV and ART may induce structural changes of HDL, thus resulting in shifts in HDL subclass distribution and HDL functionality.
    MeSH term(s) Africa South of the Sahara/epidemiology ; Anti-HIV Agents/therapeutic use ; Biomarkers/blood ; Cardiovascular Diseases/blood ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/epidemiology ; Dyslipidemias/blood ; Dyslipidemias/diagnosis ; Dyslipidemias/epidemiology ; HIV Infections/diagnosis ; HIV Infections/drug therapy ; HIV Infections/epidemiology ; HIV Long-Term Survivors ; Heart Disease Risk Factors ; Humans ; Lipoproteins, HDL/blood ; Prognosis ; Risk Assessment
    Chemical Substances Anti-HIV Agents ; Biomarkers ; Lipoproteins, HDL
    Language English
    Publishing date 2020-10-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 603838-4
    ISSN 1522-1539 ; 0363-6135
    ISSN (online) 1522-1539
    ISSN 0363-6135
    DOI 10.1152/ajpheart.00445.2020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Role of melatonin in glucose uptake by cardiomyocytes from insulin-resistant Wistar rats.

    Nduhirabandi, Frederic / Huisamen, Barbara / Strijdom, Hans / Lochner, Amanda

    Cardiovascular journal of Africa

    2017  Volume 28, Issue 6, Page(s) 362–369

    Abstract: Aim: Melatonin supplementation reduces insulin resistance and protects the heart in obese rats. However, its role in myocardial glucose uptake remains unknown. This study investigated the effect of short-term melatonin treatment on glucose uptake by ... ...

    Abstract Aim: Melatonin supplementation reduces insulin resistance and protects the heart in obese rats. However, its role in myocardial glucose uptake remains unknown. This study investigated the effect of short-term melatonin treatment on glucose uptake by cardiomyocytes isolated from obese and insulin-resistant rats.
    Methods: Cardiomyocytes were isolated from obese rats fed a high-calorie diet for 16 to 23 weeks, their age-matched controls, as well as young control rats aged four to eight weeks. After incubation with melatonin with or without insulin, glucose uptake was initiated by the addition of 2-deoxy-D- [
    Results: Obese rats (fed for more than 20 weeks) developed glucose intolerance. Cardiomyocytes isolated from these obese rats had a reduced response to insulin-stimulated glucose uptake (ISGU) (p < 0.05). Melatonin administration in vitro had no effect on glucose uptake per se. However, it increased ISGU by cardiomyocytes from the young rats (p < 0.05), while having no effect on ISGU by cardiomyocytes from the older control and obese groups. Melatonin in vivo had no significant effect on glucose tolerance, but it increased basal (p < 0.05) and ISGU by cardiomyocytes from the obese rats (50.1 ± 1.7 vs 32.1 ± 5.1 pmol/mg protein/30 min, p < 0.01).
    Conclusion: These data suggest that short-term melatonin treatment in vivo but not in vitro improved glucose uptake and insulin responsiveness of cardiomyocytes in obesity and insulin-resistance states.
    MeSH term(s) Animals ; Blood Glucose/metabolism ; Diet, High-Fat ; Dietary Supplements ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Energy Metabolism/drug effects ; Hypoglycemic Agents/pharmacology ; Insulin/pharmacology ; Insulin Resistance ; Male ; Melatonin/pharmacology ; Myocytes, Cardiac/drug effects ; Myocytes, Cardiac/metabolism ; Obesity/blood ; Obesity/drug therapy ; Obesity/physiopathology ; Rats, Wistar ; Time Factors
    Chemical Substances Blood Glucose ; Hypoglycemic Agents ; Insulin ; Melatonin (JL5DK93RCL)
    Language English
    Publishing date 2017-05-17
    Publishing country South Africa
    Document type Journal Article
    ZDB-ID 2383233-2
    ISSN 1680-0745 ; 1996-3467 ; 1015-9657 ; 1995-1892
    ISSN (online) 1680-0745 ; 1996-3467
    ISSN 1015-9657 ; 1995-1892
    DOI 10.5830/CVJA-2017-018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Cardiovascular effects of air pollution: current evidence from animal and human studies.

    Lederer, Agnes Maria / Fredriksen, Per Morten / Nkeh-Chungag, Benedicta Ngwenchi / Everson, Frans / Strijdom, Hans / De Boever, Patrick / Goswami, Nandu

    American journal of physiology. Heart and circulatory physiology

    2021  Volume 320, Issue 4, Page(s) H1417–H1439

    Abstract: Air pollution is a global health concern. Particulate matter (PM) ...

    Abstract Air pollution is a global health concern. Particulate matter (PM)
    MeSH term(s) Air Pollutants/adverse effects ; Air Pollution/adverse effects ; Animals ; Cardiovascular Diseases/chemically induced ; Cardiovascular Diseases/metabolism ; Cardiovascular Diseases/physiopathology ; Cardiovascular System/drug effects ; Cardiovascular System/metabolism ; Cardiovascular System/physiopathology ; Environmental Monitoring ; Hemodynamics/drug effects ; Humans ; Inhalation Exposure/adverse effects ; Risk Assessment ; Risk Factors ; Signal Transduction
    Chemical Substances Air Pollutants
    Language English
    Publishing date 2021-01-29
    Publishing country United States
    Document type Journal Article ; Systematic Review
    ZDB-ID 603838-4
    ISSN 1522-1539 ; 0363-6135
    ISSN (online) 1522-1539
    ISSN 0363-6135
    DOI 10.1152/ajpheart.00706.2020
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  10. Article: HIV and Antiretroviral Therapy Are Independently Associated with Cardiometabolic Variables and Cardiac Electrical Activity in Adults from the Western Cape Region of South Africa.

    Williams, Cassidy / Kamau, Festus M / Everson, Frans / Kgokane, Boipelo / De Boever, Patrick / Goswami, Nandu / Webster, Ingrid / Strijdom, Hans

    Journal of clinical medicine

    2021  Volume 10, Issue 18

    Abstract: Cardiovascular-related complications are on the rise in people with HIV/AIDS (PWH); however, the relationship among HIV and antiretroviral therapy (ART)-related parameters, cardiovascular risk, and cardiac electrical activity in PWH remain poorly studied, ...

    Abstract Cardiovascular-related complications are on the rise in people with HIV/AIDS (PWH); however, the relationship among HIV and antiretroviral therapy (ART)-related parameters, cardiovascular risk, and cardiac electrical activity in PWH remain poorly studied, especially in sub-Saharan African populations. We investigated whether HIV and ART are associated with cardiometabolic and cardiac electrical activity in PWH from Worcester in the Western Cape Province, South Africa. This was a cross-sectional study with HIV-negative (HIV-,
    Language English
    Publishing date 2021-09-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm10184112
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