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Article ; Online: Hyperbaric oxygen enhanced the chemotherapy of mitochondrial targeting molecule IR-780 in bladder cancer

Shen, Chongxing / Yue, Xiaofeng / Dai, Linyong / Wang, Jianwu / Li, Jinjin / Fang, Qiang / Zhi, Yi / Shi, Chunmeng / Li, Weibing

J Cancer Res Clin Oncol. 2023 Feb., v. 149, no. 2, p. 683-699

2023 , Page(s) 683–699

Abstract: BACKGROUND: Bladder cancer has a high rate of recurrence and drug resistance due to the lack of effective therapies. IR-780 iodide, a near-infrared (NIR) mitochondria-targeting fluorescent agent, has been demonstrated to achieve higher selectivity than ... ...

Abstract	BACKGROUND: Bladder cancer has a high rate of recurrence and drug resistance due to the lack of effective therapies. IR-780 iodide, a near-infrared (NIR) mitochondria-targeting fluorescent agent, has been demonstrated to achieve higher selectivity than other drugs in different tumor types and exhibited tumor-killing effects in some cancers. However, this therapeutic strategy is rarely studied in bladder cancer. MATERIAL AND METHODS: The accumulation of IR-780 in bladder cancer was measured by NIR imaging. Human bladder cell lines (T24, 5637, and TCCSUP) were treated with IR-780 or combined IR-780 and hyperbaric oxygen (HBO). Cell viability, cell apoptosis, cellular ATP production, mitochondrial reactive oxygen species (ROS), and plasma membrane potential were detected. Mitochondrial complex I protein NDUFS1 was measured by western blot. To confirm the anti-tumor efficacy of IR-780 + HBO, mouse bladder cell line (MB49) tumor-bearing mice were established and tumor size and weight were recorded. Besides, cell apoptosis and tumor size were assessed in drug-resistant bladder cancer cells (T24/DDP) and xenografts to evaluate the effect of IR-780 + HBO on drug-resistant bladder cancer. RESULTS: IR-780 selectively accumulated in bladder cancer (bladder cancer cells, transplanted tumors, and bladder cancer tissue from patients) and could induce cancer cell apoptosis by targeting the mitochondrial complex I protein NDUFS1. The combination with HBO could significantly enhance the anti-tumor effect of IR-780 in vitro by promoting cancer cell uptake and inducing excessive mitochondrial ROS production, while suppressing tumor growth and recurrence in animal models without causing apparent toxicity. Moreover, this combination antitumor strategy was also demonstrated in drug-resistant bladder cancer cells (T24/DDP) and xenografts. CONCLUSION: We identified for the first time a combination of IR-780 and HBO (IR-780 + HBO), which exhibits mitochondria-targeting and therapeutic capabilities, as a novel treatment paradigm for bladder cancer.
Keywords	Western blotting ; antineoplastic activity ; apoptosis ; bladder ; cell lines ; cell viability ; drug resistance ; drug therapy ; fluorescent dyes ; humans ; iodides ; membrane potential ; mice ; mitochondria ; neoplasm cells ; oxygen ; plasma membrane ; reactive oxygen species ; toxicity ; urinary bladder neoplasms ; xenotransplantation
Language	English
Dates of publication	2023-02
Size	p. 683-699
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	134792-5
ISSN	1432-1335 ; 0171-5216 ; 0084-5353 ; 0943-9382
ISSN (online)	1432-1335
ISSN	0171-5216 ; 0084-5353 ; 0943-9382
DOI	10.1007/s00432-022-04385-4
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Anti-tumor effect of mitochondrial targeted small molecule IR-780 on cisplatin-resistant bladder cancer cells

HUANG Yuandi / SHEN Chongxing / LI Jinjin / FANG Qiang / ZHI Yi

Di-san junyi daxue xuebao, Vol 43, Iss 23, Pp 2570-

2021 Volume 2576

Abstract: Objective To study the anti-tumor effect of IR-780, a mitochondrial targeting small molecule, on cisplatin-resistant T24 cell line (T24/DPP). Methods T24/DPP cell line was constructed by repeated and discontinuous stimulation of T24 cells with cisplatin. ...

Abstract	Objective To study the anti-tumor effect of IR-780, a mitochondrial targeting small molecule, on cisplatin-resistant T24 cell line (T24/DPP). Methods T24/DPP cell line was constructed by repeated and discontinuous stimulation of T24 cells with cisplatin. After the drug-resistant cells were treated with different concentrations of IR-780, cell viability was detected by CCK-8 assay and cell migration was measured by wound healing assay. The suborganelle localization of IR-780 was determined by confocal fluorescence microscopy with aid of mitotracker treatment. Cell apoptosis and production of mitochondrial reactive oxygen species (ROS) were determined by flow cytometry. T24/DPP cells were injected subcutaneously into 6-~8-week-old male nude mice to establish a tumor-bearing nude mouse model. The tumor-bearing mice were randomly divided into 3 groups, including control group (5 mg/kg PBS), DOX group (5 mg/kg DOX) and IR-780 group (5 mg/kg IR-780). After intraperitoneal administration of IR-780, the accumulation of IR-780 were detected by near-infrared in vivo imaging system and confocal fluorescence microscopy, while the tumor volume and weight were measured. Results ① In vitro, IR-780 inhibited the proliferation and migration of T24/DPP cells in a dose-dependent manner (P < 0.05). IR-780 was selectively accumulated in the mitochondria of T24/DPP cells. With the increase of IR-780 concentration, the production of mitochondrial ROS and the rate of apoptosis in T24/DDP cells were increased (P < 0.05). ② In vivo, near-infrared imaging and frozen sections of tumor tissue showed that IR-780 preferentially accumulated in the tumor of tumor-bearing nude mouse model. The tumor volume and weight of the IR-780 group were significantly less and lower when compared with the control group (P < 0.05) and DOX group (P < 0.05). Conclusion IR-780 can significantly inhibit the growth of T24/DDP cells and subcutaneous tumor xenografts in nude mice, and the mitochondrial pathway might be involved in the apoptosis of T24/DPP cells induced by IR-780.
Keywords	bladder cancer ; tumor targeting ; ir-780 ; Medicine (General) ; R5-920
Language	Chinese
Publishing date	2021-12-01T00:00:00Z
Publisher	Editorial Office of Journal of Third Military Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nocturnal oxygen therapy as an option for early COVID-19

Chongxing Shen / Xiaofeng Yue / Jianwu Wang / Chunmeng Shi / Weibing Li

International Journal of Infectious Diseases, Vol 98, Iss , Pp 176-

2020 Volume 179

Abstract: There is currently no effective antiviral therapy or immune-based treatment for coronavirus disease (COVID-19). The urgent challenge is to prevent the transition of COVID-19 from mild to severe infection. This paper discussed nocturnal oxygen therapy as ... ...

Abstract	There is currently no effective antiviral therapy or immune-based treatment for coronavirus disease (COVID-19). The urgent challenge is to prevent the transition of COVID-19 from mild to severe infection. This paper discussed nocturnal oxygen therapy as a new option for people with COVID-19 under home quarantine. It suggested that nocturnal oxygen therapy in the early stages may be helpful in preventing disease progression by inhibiting the rapid replication of the virus and improving the body's antiviral ability.
Keywords	Coronavirus ; Nocturnal oxygen therapy ; COVID-19 ; SARS-CoV-2 ; Immunomodulation ; Infectious and parasitic diseases ; RC109-216 ; covid19
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nocturnal oxygen therapy as an option for early COVID-19.

Shen, Chongxing / Yue, Xiaofeng / Wang, Jianwu / Shi, Chunmeng / Li, Weibing

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2020 Volume 98, Page(s) 176–179

Abstract	There is currently no effective antiviral therapy or immune-based treatment for coronavirus disease (COVID-19). The urgent challenge is to prevent the transition of COVID-19 from mild to severe infection. This paper discussed nocturnal oxygen therapy as a new option for people with COVID-19 under home quarantine. It suggested that nocturnal oxygen therapy in the early stages may be helpful in preventing disease progression by inhibiting the rapid replication of the virus and improving the body's antiviral ability.
MeSH term(s)	Betacoronavirus ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/therapy ; Humans ; Hypoxia/etiology ; Hypoxia/therapy ; Oxygen/therapeutic use ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/therapy ; SARS-CoV-2
Chemical Substances	Oxygen (S88TT14065)
Keywords	covid19
Language	English
Publishing date	2020-06-26
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2020.06.080
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Pharmaceutical Manipulation of Mitochondrial F0F1-ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress-induced Selective Enrichment.

Chen, Zelin / Tan, Xu / Jin, Taotao / Wang, Yu / Dai, Linyong / Shen, Gufang / Zhang, Can / Qu, Langfan / Long, Lei / Shen, Chongxing / Cao, Xiaohui / Wang, Jianwu / Li, Huijuan / Yue, Xiaofeng / Shi, Chunmeng

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2023 Volume 11, Issue 9, Page(s) e2307880

Abstract: To rescue ischemic myocardium from progressing to myocardial infarction, timely identification of the infarct size and reperfusion is crucial. However, fast and accurate identification, as well as the targeted protection of injured cardiomyocytes ... ...

Abstract	To rescue ischemic myocardium from progressing to myocardial infarction, timely identification of the infarct size and reperfusion is crucial. However, fast and accurate identification, as well as the targeted protection of injured cardiomyocytes following ischemia/reperfusion (I/R) injury, remain significantly challenging. Here, a near infrared heptamethine dye IR-780 is shown that has the potential to quickly monitor the area at risk following I/R injury by selectively entering the cardiomyocytes of the at-risk heart tissues. Preconditioning with IR-780 or timely IR-780 administration before reperfusion significantly protects the heart from ischemia and oxidative stress-induced cell death, myocardial remodeling, and heart failure in both rat and pig models. Furthermore, IR-780 can directly bind to F0F1-ATP synthase of cardiomyocytes, rapidly decrease the mitochondrial membrane potential, and subsequently slow down the mitochondrial energy metabolism, which induces the mitochondria into a "quiescent state" and results in mitochondrial permeability transition pore inhibition by preventing mitochondrial calcium overload. Collectively, the findings show the feasibility of IR-780-based imaging and protection strategy for I/R injury in a preclinical context and indicate that moderate mitochondrial function depression is a mode of action that can be targeted in the development of cardioprotective reagents.
MeSH term(s)	Rats ; Animals ; Swine ; Myocardial Reperfusion Injury/prevention & control ; Myocardial Reperfusion Injury/metabolism ; Mitochondrial Proton-Translocating ATPases/metabolism ; Pharmaceutical Preparations ; Myocytes, Cardiac/metabolism ; Myocardial Infarction/metabolism ; Adenosine Triphosphate/metabolism
Chemical Substances	Mitochondrial Proton-Translocating ATPases (EC 3.6.3.-) ; Pharmaceutical Preparations ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2023-12-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202307880
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Hyaluronic Acid-Conjugated Fluorescent Probe-Shielded Polydopamine Nanomedicines for Targeted Imaging and Chemotherapy of Bladder Cancer.

Qiu, Heping / Wang, Jianwu / Zhi, Yi / Yan, Benhuang / Huang, Yuandi / Li, Jinjin / Shen, Chongxing / Dai, Linyong / Fang, Qiang / Shi, Chunmeng / Li, Weibing

ACS applied materials & interfaces

2023 Volume 15, Issue 40, Page(s) 46668–46680

Abstract: Bladder cancer is one of the most common malignancies in the urinary system, with high risk of recurrence and progression. However, the difficulty in detecting small tumor lesions and the lack of selectivity of intravesical treatment seriously affect the ...

Abstract	Bladder cancer is one of the most common malignancies in the urinary system, with high risk of recurrence and progression. However, the difficulty in detecting small tumor lesions and the lack of selectivity of intravesical treatment seriously affect the prognosis of patients with bladder cancer. In the present work, a nanoparticle-based delivery system with tumor targeting, high biocompatibility, simple preparation, and the ability to synergize imaging and therapy was fabricated. Specifically, this nanosystem consisted of the core of doxorubicin (DOX)-loaded polydopamine nanoparticles (PDD NPs) and the shell of hyaluronic acid (HA)-conjugated IR780 (HA-IR780). The HA-IR780-covered PDD NPs (HR-PDD NPs) demonstrated tumor targeting and visualization both in vitro and in vivo with properties of promoted cancer cell endocytosis and lysosomal escape, efficiently delivering drugs to the target site and exerting a killing effect on tumor cells. Encouragingly, intravesical instillation of HR-PDD NPs improved drug retention in the bladder and promoted its accumulation in tumor tissue, resulting in better tumor proliferation inhibition and apoptosis in an orthotopic bladder cancer model in rats. This study provides a promising strategy for the diagnosis and therapy of bladder cancer.
Language	English
Publishing date	2023-09-28
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c09564
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Nocturnal oxygen therapy as an option for early COVID-19

Shen, Chongxing / Yue, Xiaofeng / Wang, Jianwu / Shi, Chunmeng / Li, Weibing

International Journal of Infectious Diseases

2020 Volume 98, Page(s) 176–179

Keywords	Microbiology (medical) ; Infectious Diseases ; General Medicine ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2020.06.080
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hyperbaric oxygen enhanced the chemotherapy of mitochondrial targeting molecule IR-780 in bladder cancer.

Shen, Chongxing / Yue, Xiaofeng / Dai, Linyong / Wang, Jianwu / Li, Jinjin / Fang, Qiang / Zhi, Yi / Shi, Chunmeng / Li, Weibing

Journal of cancer research and clinical oncology

2022 Volume 149, Issue 2, Page(s) 683–699

Abstract: Background: Bladder cancer has a high rate of recurrence and drug resistance due to the lack of effective therapies. IR-780 iodide, a near-infrared (NIR) mitochondria-targeting fluorescent agent, has been demonstrated to achieve higher selectivity than ... ...

Abstract	Background: Bladder cancer has a high rate of recurrence and drug resistance due to the lack of effective therapies. IR-780 iodide, a near-infrared (NIR) mitochondria-targeting fluorescent agent, has been demonstrated to achieve higher selectivity than other drugs in different tumor types and exhibited tumor-killing effects in some cancers. However, this therapeutic strategy is rarely studied in bladder cancer. Material and methods: The accumulation of IR-780 in bladder cancer was measured by NIR imaging. Human bladder cell lines (T24, 5637, and TCCSUP) were treated with IR-780 or combined IR-780 and hyperbaric oxygen (HBO). Cell viability, cell apoptosis, cellular ATP production, mitochondrial reactive oxygen species (ROS), and plasma membrane potential were detected. Mitochondrial complex I protein NDUFS1 was measured by western blot. To confirm the anti-tumor efficacy of IR-780 + HBO, mouse bladder cell line (MB49) tumor-bearing mice were established and tumor size and weight were recorded. Besides, cell apoptosis and tumor size were assessed in drug-resistant bladder cancer cells (T24/DDP) and xenografts to evaluate the effect of IR-780 + HBO on drug-resistant bladder cancer. Results: IR-780 selectively accumulated in bladder cancer (bladder cancer cells, transplanted tumors, and bladder cancer tissue from patients) and could induce cancer cell apoptosis by targeting the mitochondrial complex I protein NDUFS1. The combination with HBO could significantly enhance the anti-tumor effect of IR-780 in vitro by promoting cancer cell uptake and inducing excessive mitochondrial ROS production, while suppressing tumor growth and recurrence in animal models without causing apparent toxicity. Moreover, this combination antitumor strategy was also demonstrated in drug-resistant bladder cancer cells (T24/DDP) and xenografts. Conclusion: We identified for the first time a combination of IR-780 and HBO (IR-780 + HBO), which exhibits mitochondria-targeting and therapeutic capabilities, as a novel treatment paradigm for bladder cancer.
MeSH term(s)	Humans ; Animals ; Mice ; Reactive Oxygen Species/metabolism ; Hyperbaric Oxygenation ; Urinary Bladder Neoplasms/drug therapy ; Urinary Bladder Neoplasms/metabolism ; Apoptosis ; Radiation-Sensitizing Agents/pharmacology ; Mitochondria ; Cell Line, Tumor
Chemical Substances	2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium ; Reactive Oxygen Species ; Radiation-Sensitizing Agents
Language	English
Publishing date	2022-11-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	134792-5
ISSN	1432-1335 ; 0171-5216 ; 0084-5353 ; 0943-9382
ISSN (online)	1432-1335
ISSN	0171-5216 ; 0084-5353 ; 0943-9382
DOI	10.1007/s00432-022-04385-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique.

Shen, Xinkun / Hu, Wenjia / Ping, Linchao / Liu, Chongxing / Yao, Lili / Deng, Zhennan / Wu, Gang

Frontiers in bioengineering and biotechnology

2020 Volume 8, Page(s) 573464

Abstract: Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy ...

Abstract	Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidation (MAO) significantly improved the bioactivity and coating stability of Ti-based substrates. In this study, we further functionalized Ti with antibacterial and osteogenic properties by doping silicon (Si) and/or copper (Cu) ions into HESP/MAO-treated coatings. Physicochemical characterization displayed that the doping of Si and Cu in HESP/MAO-treated coatings (Si/Cu-MAO) did not significantly change their surface topography, roughness, crystal structure, coating thickness, bonding strength, and wettability. The results of X-ray photoelectron spectroscopy (XPS) showed that Si and Cu in the Si/Cu-MAO coating was in the form of silicate radical (SiO
Language	English
Publishing date	2020-10-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2020.573464
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Nocturnal oxygen therapy as an option for early COVID-19

Shen, Chongxing / Yue, Xiaofeng / Wang, Jianwu / Shi, Chunmeng / Li, Weibing

Int J Infect Dis

Abstract	There is currently no effective antiviral therapy or immune-based treatment for coronavirus disease (COVID-19). The urgent challenge is to prevent the transition of COVID-19 from mild to severe infection. This paper discussed nocturnal oxygen therapy as a new option for people with COVID-19 under home quarantine. It suggested that nocturnal oxygen therapy in the early stages may be helpful in preventing disease progression by inhibiting the rapid replication of the virus and improving the body's antiviral ability.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #620832
Database	COVID19

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