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Article ; Online: COVID-19 Detection Using Contemporary Biosensors and Machine Learning Approach: A Review.

Agarwal, Sajal / Srivastava, Rupam / Kumar, Santosh / Prajapati, Yogendra Kumar

IEEE transactions on nanobioscience

2024 Volume 23, Issue 2, Page(s) 291–299

Abstract: The current global pandemic not only claims countless human lives but also rocks the economies of every country on the planet. This fact needs the development of novel, productive, and efficient techniques to detect the SARS-CoV-2 virus. This review ... ...

Abstract	The current global pandemic not only claims countless human lives but also rocks the economies of every country on the planet. This fact needs the development of novel, productive, and efficient techniques to detect the SARS-CoV-2 virus. This review article discusses the current state of SARS-CoV-2 virus detection methods such as electrochemical, fluorescent, and electronic, etc., as well as the potential of optical sensors with a wide range of novel approaches and models. This review provides a comprehensive comparison of various detection methods by comparing the various techniques in depth. In addition, there is a brief discussion of the futuristic approach combining optical sensors with machine learning algorithms. It is believed that this study would prove to be critical for the scientific community to explore solutions for detecting viruses with improved functionality.
MeSH term(s)	Humans ; COVID-19/diagnosis ; SARS-CoV-2 ; Algorithms ; Machine Learning ; Pandemics ; Biosensing Techniques
Language	English
Publishing date	2024-03-28
Publishing country	United States
Document type	Review ; Journal Article
ISSN	1558-2639
ISSN (online)	1558-2639
DOI	10.1109/TNB.2023.3342126
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Article: Research Trend and Detailed Insights into the Molecular Mechanisms of Food Bioactive Compounds against Cancer: A Comprehensive Review with Special Emphasis on Probiotics.

Agrawal, Manas Yogendra / Gaikwad, Shreyas / Srivastava, Sangeeta / Srivastava, Sanjay K

Cancers

2022 Volume 14, Issue 22

Abstract: In an attempt to find a potential cure for cancer, scientists have been probing the efficacy of the food we eat and its bioactive components. Over the decades, there has been an exponentially increasing trend of research correlating food and cancer. This ...

Abstract	In an attempt to find a potential cure for cancer, scientists have been probing the efficacy of the food we eat and its bioactive components. Over the decades, there has been an exponentially increasing trend of research correlating food and cancer. This review explains the molecular mechanisms by which bioactive food components exhibit anticancer effects in several cancer models. These bioactive compounds are mainly plant based or microbiome based. While plants remain the primary source of these phytochemicals, little is known about probiotics, i.e., microbiome sources, and their relationships with cancer. Thus, the molecular mechanisms underlying the anticancer effect of probiotics are discussed in this review. The principal mode of cell death for most food bioactives is found to be apoptosis. Principal oncogenic signaling axes such as Akt/PI3K, JAK/STAT, and NF-κB seem to be modulated due to these bioactives along with certain novel targets that provide a platform for further oncogenic research. It has been observed that probiotics have an immunomodulatory effect leading to their chemopreventive actions. Various foods exhibit better efficacy as complete extracts than their individual phytochemicals, indicating an orchestrated effect of the food components. Combining bioactive agents with available chemotherapies helps synergize the anticancer action of both to overcome drug resistance. Novel techniques to deliver bioactive agents enhance their therapeutic response. Such combinations and novel approaches are also discussed in this review. Notably, most of the food components that have been studied for cancer have shown their efficacy in vivo. This bolsters the claims of these studies and, thus, provides us with hope of discovering anticancer agents in the food that we eat.
Language	English
Publishing date	2022-11-08
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers14225482
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Article ; Online: Interferon stimulated gene 15 (ISG15) in cancer: An update.

Nguyen, Hong-My / Gaikwad, Shreyas / Oladejo, Mariam / Agrawal, Manas Yogendra / Srivastava, Sanjay K / Wood, Laurence M

Cancer letters

2023 Volume 556, Page(s) 216080

Abstract: Among the plethora of defense mechanisms which a host elicits after pathogen invasion, type 1 interferons play a central role in regulating the immune system's response. They induce several interferon-stimulated genes (ISGs) which play a diverse role ... ...

Abstract	Among the plethora of defense mechanisms which a host elicits after pathogen invasion, type 1 interferons play a central role in regulating the immune system's response. They induce several interferon-stimulated genes (ISGs) which play a diverse role once activated. Over the past few decades, there have been several studies exploring the role of ISGs in cancer and ISG15 is among the most studied for its pro and anti-tumorigenic role. In this review, we aim to provide an update on the recent observations and findings related to ISG15 in cancer. We provide a brief overview about the initial observations and important historical findings which helped scientists understand structure and function of ISG15. We aim to provide an overview of ISG15 in cancer with an emphasis on studies which delve into the molecular mechanism of ISG15 in modulating the tumor microenvironment. Further, the dysregulation of ISG15 in cancer and the molecular mechanisms associated with its pro and anti-tumor roles are discussed in respective cancer types. Finally, we discuss multiple therapeutic applications of ISG15 in current cancer therapy.
MeSH term(s)	Humans ; Cytokines/genetics ; Interferon Type I/genetics ; Neoplasms/drug therapy ; Neoplasms/genetics ; Tumor Microenvironment ; Ubiquitins/genetics
Chemical Substances	Cytokines ; Interferon Type I ; ISG15 protein, human (60267-61-0) ; Ubiquitins
Language	English
Publishing date	2023-02-01
Publishing country	Ireland
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural
ZDB-ID	195674-7
ISSN	1872-7980 ; 0304-3835
ISSN (online)	1872-7980
ISSN	0304-3835
DOI	10.1016/j.canlet.2023.216080
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Article: A Study on the Attitude and Perception of Bachelor of Medicine and Bachelor of Surgery (MBBS) Students Regarding Post-MBBS Compulsory Service Bond in Uttar Pradesh, India.

Tiwari, Harish Chandra / Mishra, Richa / Singh, Yogendra / Khan, Imran Ahmed / Srivastava, Dhirendra K

Cureus

2023 Volume 15, Issue 9, Page(s) e44682

Abstract: Background To increase the availability of doctors in the public healthcare delivery system, the state government of Uttar Pradesh, India, has implemented a two-year compulsory service bond since 2018. Students of the 2018 batch are going to complete ... ...

Abstract	Background To increase the availability of doctors in the public healthcare delivery system, the state government of Uttar Pradesh, India, has implemented a two-year compulsory service bond since 2018. Students of the 2018 batch are going to complete their Bachelor of Medicine, Bachelor of Surgery (MBBS) in 2023 and are supposed to serve through this bond. There are many dilemmas in the minds of medical students regarding their compulsory service bond. Hence, there is a need to know their attitude and perceptions regarding the compulsory service bond. This study was conducted to assess the attitude and perception of undergraduate medical students toward compulsory service bonds. Methods This was a mixed-method study conducted in July-September 2022 among undergraduate medical students at Baba Raghav Das Medical College, Gorakhpur, Uttar Pradesh, India. For quantitative data, a structured questionnaire was developed using Google Forms (Google LLC, Mountain View, California, United States) and circulated via WhatsApp (Meta Platforms, Inc., Menlo Park, California, United States) through the random sampling method. Focused group discussions were carried out to collect the qualitative data. Result Regarding the compulsory service bond after MBBS, 100 (31.8%) medical students were found to be interested and 56 (17.8%) were disinterested. The majority (n=158; 50.4%) of participants were neutral, while 278 (88.6%) medical students perceived it as an opportunity to help poor people. Higher possibilities of social recognition and respect were some noticeable perceptions of 243 (77.4%) MBBS students. Lack of confidence to tackle serious cases without a senior doctor's supervision was perceived as an important hurdle by 286 (91%) participants. Non-availability of advanced medical facilities, issues like the safety of doctors, and the lack of availability of electricity, roads, and infrastructure were also perceived as hurdles. Conclusions and recommendations Students perceived the compulsory service bond as an opportunity if met with certain conditions like a transparent method of posting and basic facilities or an incentive for accommodation and transportation. The compulsory service bond for addressing the shortfall of doctors in the public healthcare delivery system may be more effective if these hurdles are corrected and certain opportunities are met, as mentioned in the present study. This will help the government move smoothly towards achieving Universal Health Coverage (UHC).
Language	English
Publishing date	2023-09-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.44682
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Article: Nanocarbon Reinforced Rubber Nanocomposites: Detailed Insights about Mechanical, Dynamical Mechanical Properties, Payne, and Mullin Effects.

Srivastava, Suneel Kumar / Mishra, Yogendra Kumar

Nanomaterials (Basel, Switzerland)

2018 Volume 8, Issue 11

Abstract: The reinforcing ability of the fillers results in significant improvements in properties of polymer matrix at extremely low filler loadings as compared to conventional fillers. In view of this, the present review article describes the different methods ... ...

Abstract	The reinforcing ability of the fillers results in significant improvements in properties of polymer matrix at extremely low filler loadings as compared to conventional fillers. In view of this, the present review article describes the different methods used in preparation of different rubber nanocomposites reinforced with nanodimensional individual carbonaceous fillers, such as graphene, expanded graphite, single walled carbon nanotubes, multiwalled carbon nanotubes and graphite oxide, graphene oxide, and hybrid fillers consisting combination of individual fillers. This is followed by review of mechanical properties (tensile strength, elongation at break, Young modulus, and fracture toughness) and dynamic mechanical properties (glass transition temperature, crystallization temperature, melting point) of these rubber nanocomposites. Finally, Payne and Mullin effects have also been reviewed in rubber filled with different carbon based nanofillers.
Language	English
Publishing date	2018-11-16
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano8110945
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Article ; Online: Multifarious Plant Growth-Promoting Rhizobacterium

Sharma, Anjney / Chakdar, Hillol / Vaishnav, Anukool / Srivastava, Alok Kumar / Khan, Naeem / Bansal, Yogendra Kumar / Kaushik, Rajeev

Frontiers in bioscience (Landmark edition)

2023 Volume 28, Issue 10, Page(s) 241

Abstract: Background: Chickpea is one of the most important leguminous crops and its productivity is significantly affected by salinity stress. The use of ecofriendly, salt-tolerant, plant growth-promoting rhizobacteria (PGPR) as a bioinoculant can be very ... ...

Abstract	Background: Chickpea is one of the most important leguminous crops and its productivity is significantly affected by salinity stress. The use of ecofriendly, salt-tolerant, plant growth-promoting rhizobacteria (PGPR) as a bioinoculant can be very effective in mitigating salinity stress in crop plants. In the present study, we explored, characterized, and evaluated a potential PGPR isolate for improving chickpea growth under salt stress. Methods: A potential PGPR was isolated from rhizospheric soils of chickpea plants grown in the salt-affected area of eastern Uttar Pradesh, India. The isolate was screened for salt tolerance and characterized for its metabolic potential and different plant growth-promoting attributes. Further, the potential of the isolate to promote chickpea growth under different salt concentrations was determined by a greenhouse experiment. Results: A rhizobacteria isolate, CM94, which could tolerate a NaCl concentration of up to 8% was selected for this study. Based on the BIOLOG carbon source utilization, isolate CM94 was metabolically versatile and able to produce multiple plant growth-promoting attributes, such as indole acetic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophore, hydrogen cyanide (HCN), and ammonia as well as solubilized phosphate. A polyphasic approach involving the analysis of fatty acid methyl ester (FAME) and 16S rRNA gene sequencing confirmed the identity of the isolate as Conclusions: Overall, the results suggested that using
MeSH term(s)	Cicer/genetics ; Cicer/metabolism ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/metabolism ; Plant Development ; Soil ; Salt Tolerance
Chemical Substances	RNA, Ribosomal, 16S ; Soil
Language	English
Publishing date	2023-11-02
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2704569-9
ISSN	2768-6698 ; 2768-6698
ISSN (online)	2768-6698
ISSN	2768-6698
DOI	10.31083/j.fbl2810241
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Article ; Online: Immune checkpoint proteins: Signaling mechanisms and molecular interactions in cancer immunotherapy.

Gaikwad, Shreyas / Agrawal, Manas Yogendra / Kaushik, Itishree / Ramachandran, Sharavan / Srivastava, Sanjay K

Seminars in cancer biology

2022 Volume 86, Issue Pt 3, Page(s) 137–150

Abstract: Immune checkpoint proteins (ICP) are currently one of the most novel and promising areas of immune-oncology research. This novel way of targeting tumor cells has shown favorable success over the past few years with some FDA approvals such as Ipilimumab, ... ...

Abstract	Immune checkpoint proteins (ICP) are currently one of the most novel and promising areas of immune-oncology research. This novel way of targeting tumor cells has shown favorable success over the past few years with some FDA approvals such as Ipilimumab, Nivolumab, Pembrolizumab etc. Currently, more than 3000 clinical trials of immunotherapeutic agents are ongoing with majority being ICPs. However, as the number of trials increase so do the challenges. Some challenges such as adverse side effects, non-specific binding on healthy tissues and absence of response in some subset populations are critical obstacles. For a safe and effective further therapeutic development of molecules targeting ICPs, understanding their mechanism at molecular level is crucial. Since ICPs are mostly membrane bound receptors, a number of downstream signaling pathways divaricate following ligand-receptor binding. Most ICPs are expressed on more than one type of immune cell populations. Further, the expression varies within a cell type. This naturally varied expression pattern adds to the difficulty of targeting specific effector immune cell types against cancer. Hence, understanding the expression pattern and cellular mechanism helps lay out the possible effect of any immunotherapy. In this review, we discuss the signaling mechanism, expression pattern among various immune cells and molecular interactions derived using interaction database analysis (BioGRID).
MeSH term(s)	Humans ; Immune Checkpoint Proteins ; Programmed Cell Death 1 Receptor ; Immunotherapy ; CTLA-4 Antigen ; Neoplasms/therapy ; Immunologic Factors
Chemical Substances	Immune Checkpoint Proteins ; Programmed Cell Death 1 Receptor ; CTLA-4 Antigen ; Immunologic Factors
Language	English
Publishing date	2022-03-24
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1033980-2
ISSN	1096-3650 ; 1044-579X
ISSN (online)	1096-3650
ISSN	1044-579X
DOI	10.1016/j.semcancer.2022.03.014
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Article ; Online: Multifarious Plant Growth-Promoting Rhizobacterium Enterobacter sp. CM94-Mediated Systemic Tolerance and Growth Promotion ofChickpea (Cicer arietinum L.) under Salinity Stress

Anjney Sharma / Hillol Chakdar / Anukool Vaishnav / Alok Kumar Srivastava / Naeem Khan / Yogendra Kumar Bansal / Rajeev Kaushik

Frontiers in Bioscience-Landmark, Vol 28, Iss 10, p

2023 Volume 241

Abstract	Background: Chickpea is one of the most important leguminous crops and its productivity is significantly affected by salinity stress. The use of ecofriendly, salt-tolerant, plant growth-promoting rhizobacteria (PGPR) as a bioinoculant can be very effective in mitigating salinity stress in crop plants. In the present study, we explored, characterized, and evaluated a potential PGPR isolate for improving chickpea growth under salt stress. Methods: A potential PGPR was isolated from rhizospheric soils of chickpea plants grown in the salt-affected area of eastern Uttar Pradesh, India. The isolate was screened for salt tolerance and characterized for its metabolic potential and different plant growth-promoting attributes. Further, the potential of the isolate to promote chickpea growth under different salt concentrations was determined by a greenhouse experiment. Results: A rhizobacteria isolate, CM94, which could tolerate a NaCl concentration of up to 8% was selected for this study. Based on the BIOLOG carbon source utilization, isolate CM94 was metabolically versatile and able to produce multiple plant growth-promoting attributes, such as indole acetic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophore, hydrogen cyanide (HCN), and ammonia as well as solubilized phosphate. A polyphasic approach involving the analysis of fatty acid methyl ester (FAME) and 16S rRNA gene sequencing confirmed the identity of the isolate as Enterobacter sp. The results of greenhouse experiments revealed that isolate CM94 inoculation significantly enhanced the shoot length, root length, and fresh and dry weight of chickpea plants, under variable salinity stress. In addition, inoculation improved the chlorophyll, proline, sugar, and protein content in the tissues of the plant, while lowering lipid peroxidation. Furthermore, isolate CM94 reduced oxidative stress by enhancing the enzymatic activities of superoxide dismutase, catalase, and peroxidase compared to in the respective uninoculated plants. Conclusions: ...
Keywords	pgpr ; chickpea ; salt tolerance ; lipid peroxidation ; antioxidant enzymes ; Biochemistry ; QD415-436 ; Biology (General) ; QH301-705.5
Subject code	580
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	IMR Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Assessment of Usefulness of CT Scan in AO Classification of Intertrochanteric Fractures: A Prospective Observational Study.

Raj, Rahul Yogendra / Srivastava, Amit / Aggarwal, Aditya Nath / Haq, Rehan Ul

Indian journal of orthopaedics

2021 Volume 56, Issue 3, Page(s) 392–398

Abstract: Objective: The sliding hip screw (SHS) is the gold standard for the management of stable intertrochanteric (IT) fractures. However, intramedullary implants are now being increasingly used for management of unstable IT fractures especially those with a ... ...

Abstract	Objective: The sliding hip screw (SHS) is the gold standard for the management of stable intertrochanteric (IT) fractures. However, intramedullary implants are now being increasingly used for management of unstable IT fractures especially those with a compromised or vulnerable lateral wall. Therefore, accurate classification of fracture is important to ensure proper surgical planning and choice of implant. The AO classification for IT fractures is based on plain radiographs alone and many authors have reported it to have poor inter- and intra-observer agreement. Therefore, the objectives of the study were to assess the improvement in inter- and intra-observer agreement of the AO classification after addition of CT scan to plain radiographs, to assess the change in pattern of AO classification on addition of CT scan to plain radiographs and to assess percentage of times, stable lateral wall seen on plain radiographs is classified as unstable or broken on CT scans. Method: Fifty-four patients of intertrochanteric fracture were included in study. Plain radiographs of patients were shown to three orthopedic surgeons. They were asked to document the AO classification of the fracture, and comment on the integrity of the lateral wall. Then, CT scans with 3D reconstructions of the same patients were provided along with the radiographs and they were asked to classify the fracture again. Inter- and intra-observer agreement of the AO classification based on plain radiographs alone and once CT scan with 3D reconstruction was added to the plain radiographs was determined using Kappa coefficients. Pattern of change in classification on addition of CT scan to plain radiographs was also assessed. Result: The mean kappa value for inter- and intra-observer agreement for AO classification on plain radiographs alone were 0.58 (moderate) and 0.66 (substantial), respectively. Upon addition of CT scan to plain radiographs, both improved to 0.70 (substantial) and 0.77 (substantial), respectively. The AO classification of the fracture changed 28.70% times (93 of 324 observations) upon addition of CT scan to plain radiographs. 96.77% times (90 of 93 observations) the classification was upgraded to higher group, while it was downgraded in only 3.22% times (3 of 93). 55.91% times this change was observed in AO 31 A 2 group (52 of 93 observations). In 17.59% cases (57 of 324 observations), fractures which were classified as stable (A1.1-A2.1) on radiographs alone, were reclassified as unstable (A2.2-3.3) upon addition of CT scan to plain radiographs. In 11.4% cases (37 of 324 observations), the lateral wall was classified as vulnerable or broken on CT scans where it was classified intact on plain radiographs. Conclusion: Addition of CT scans with 3D reconstructions to plain radiographs improves the intra- and inter-observer agreement of the AO classification. Addition of CT scan results in change in classification of the fracture in about one out of three cases. This most commonly happens in the AO 31 A 2 group. Most of the times, this results in the classification of fracture being upgraded. Many fractures which are initially classified as stable (A1.1-A2.1) on radiographs are reclassified as unstable (A2.2-3.3) on addition of CT scans. The lateral wall is also classified as vulnerable or broken more number of times on CT scans than plain radiographs alone. Therefore, we conclude that CT scan with 3 D reconstructions definitely helps in better pre-operative classification of intertrochanteric fractures especially in select group of fractures (AO 31 A 2) where stability and integrity of lateral wall is difficult to assess.
Language	English
Publishing date	2021-10-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	603194-8
ISSN	0019-5413
ISSN	0019-5413
DOI	10.1007/s43465-021-00522-2
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Article ; Online: Nanocarbon Reinforced Rubber Nanocomposites

Suneel Kumar Srivastava / Yogendra Kumar Mishra

Nanomaterials, Vol 8, Iss 11, p

Detailed Insights about Mechanical, Dynamical Mechanical Properties, Payne, and Mullin Effects

2018 Volume 945

Abstract	The reinforcing ability of the fillers results in significant improvements in properties of polymer matrix at extremely low filler loadings as compared to conventional fillers. In view of this, the present review article describes the different methods used in preparation of different rubber nanocomposites reinforced with nanodimensional individual carbonaceous fillers, such as graphene, expanded graphite, single walled carbon nanotubes, multiwalled carbon nanotubes and graphite oxide, graphene oxide, and hybrid fillers consisting combination of individual fillers. This is followed by review of mechanical properties (tensile strength, elongation at break, Young modulus, and fracture toughness) and dynamic mechanical properties (glass transition temperature, crystallization temperature, melting point) of these rubber nanocomposites. Finally, Payne and Mullin effects have also been reviewed in rubber filled with different carbon based nanofillers.
Keywords	rubber nanocomposites ; mechanical properties ; dynamical mechanical properties ; Payne effect ; Mullin effect ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2018-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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