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Article: Cross-coupling of organic fluorides with allenes: a silyl-radical-relay pathway for the construction of α-alkynyl-substituted all-carbon quaternary centres.

Zhou, Jun / Zhao, Zhengyu / Mori, Soichiro / Yamamoto, Katsuhiro / Shibata, Norio

2024 Volume 15, Issue 14, Page(s) 5113–5122

Abstract: Controlling the transformation of versatile and reactive allenes is a considerable challenge. Herein, we report an efficient silylboronate-mediated cross-coupling reaction of organic fluorides with allenes to construct a series of sterically demanding α- ... ...

Abstract	Controlling the transformation of versatile and reactive allenes is a considerable challenge. Herein, we report an efficient silylboronate-mediated cross-coupling reaction of organic fluorides with allenes to construct a series of sterically demanding α-ethynyl-containing all-carbon quaternary centers (ACQCs), using catalyst-free silyl-radical-relay reactions to selectively functionalize highly inert C-F bonds in organic fluorides. The key to the success of this transformation lies in the radical rearrangement of an
Language	English
Publishing date	2024-03-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/d3sc06617g
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Article ; Online: Case of giant juvenile angiofibroma resected by external incision with temporary double balloon occlusion of the internal carotid artery by intraoperative endovascular treatment.

Maeda-Anezaki, Mayuka / Omura, Kazuhiro / Mori, Ryosuke / Ishida, Katsuhiro

BMJ case reports

2024 Volume 17, Issue 3

Abstract: We report the first case of a juvenile nasal angiofibroma (JNA) fed by multiple arteries from the internal carotid artery (ICA), removed without complications by temporarily blocking the ICA with two balloons. An early adolescent with JNA underwent ... ...

Abstract	We report the first case of a juvenile nasal angiofibroma (JNA) fed by multiple arteries from the internal carotid artery (ICA), removed without complications by temporarily blocking the ICA with two balloons. An early adolescent with JNA underwent preoperative embolisation of feeding arteries arising from the external carotid artery (ECA) (University of Pittsburgh Medical Centre classification IV). Endoscopic resection was attempted once but discontinued due to massive bleeding (7000 mL). 17 months later, the JNA had grown to fill both nasal cavities. Repeated preoperative embolisation of the feeders from the ECA was performed, followed by surgery combined with endoscopic and external incision. Intraoperatively, two balloons were inserted into the right ICA, which were inflated at the proximal and distal sites of the feeder vessels to cut-off blood flow to the tumour. The tumour was almost completely resected with 6270 mL of blood loss and no postoperative neurological deterioration.
MeSH term(s)	Adolescent ; Humans ; Carotid Artery, Internal/diagnostic imaging ; Carotid Artery, Internal/surgery ; Angiofibroma/complications ; Treatment Outcome ; Balloon Occlusion ; Embolization, Therapeutic ; Nasopharyngeal Neoplasms/complications ; Head and Neck Neoplasms/complications ; Carotid Artery, External/surgery
Language	English
Publishing date	2024-03-13
Publishing country	England
Document type	Case Reports ; Journal Article
ISSN	1757-790X
ISSN (online)	1757-790X
DOI	10.1136/bcr-2023-256731
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Article ; Online: Retrospective Comparative Study of Clinical Outcomes and Cost-Effectiveness with Bone Substitutes on Volar Locking Plate Fixation of Unstable Distal Radial Fractures in the Elderly.

Mori, Yusuke / Takegami, Yasuhiko / Tokutake, Katsuhiro / Oka, Yoshiharu / Imagama, Shiro

The journal of hand surgery Asian-Pacific volume

2023 Volume 28, Issue 1, Page(s) 61–68

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Humans ; Aged ; Retrospective Studies ; Radius Fractures/diagnostic imaging ; Radius Fractures/surgery ; Bone Substitutes/therapeutic use ; Cost-Benefit Analysis ; Radius ; Wrist Fractures
Chemical Substances	Bone Substitutes
Language	English
Publishing date	2023-02-20
Publishing country	Singapore
Document type	Multicenter Study ; Journal Article
ZDB-ID	2848651-1
ISSN	2424-8363 ; 2424-8355
ISSN (online)	2424-8363
ISSN	2424-8355
DOI	10.1142/S2424835523500091
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Article ; Online: Establishment of Membrane-Bound IgA1-Specific Antibody Possessing Antibody-Dependent Cellular Cytotoxicity Activity.

Yamasaki, Kohei / Kaneko, Etsuji / Mori, Katsuhiro

Monoclonal antibodies in immunodiagnosis and immunotherapy

2022 Volume 41, Issue 3, Page(s) 125–132

Abstract: Therapeutic agents targeting B cells, such as rituximab, have been proven to be effective in several diseases. However, since this therapy targets the whole B cell population, there are still concerns about critical adverse events. Therefore, a new type ... ...

Abstract	Therapeutic agents targeting B cells, such as rituximab, have been proven to be effective in several diseases. However, since this therapy targets the whole B cell population, there are still concerns about critical adverse events. Therefore, a new type of antibody that can specifically deplete a particular type of B cell may have the potential to become an efficient and safer therapy. Membrane-bound IgA1 (mIgA1) is expressed on soluble IgA1 (sIgA1) producing B cells and/or their precursor, B cells. Although most of the amino acid sequence in the N-terminal regions of the extracellular domains of mIgA1 and sIgA1 is shared, there is a membrane type-specific region, named the membrane-bound immunoglobulin isotype-specific (migis-α) region, at the membrane-proximal region of mIgA1. We hypothesized that the migis-α region would be a suitable antigen for therapeutic antibodies to target mIgA1-expressing B cells without binding to sIgA1, which may cause undesired adverse effects and poor pharmacokinetics (PK). We established two anti-migis-α monoclonal antibodies (mAbs), KM4641 and KM4644, by immunization of the synthetic peptide corresponding to an migis-α region. These mAbs both showed robust binding to mIgA1-expressing transfectant cells. As we expected, neither mAbs bound to sIgA1 and we found that the mAbs recognized different seven to eight amino acid sequences within the migis-α region. Furthermore, the rat-human chimeric type of both mAbs showed antibody-dependent cellular cytotoxicity against mIgA1-expressing transfectant cells. Taken together, this study showed that established mAbs have therapeutic potential in IgA-related diseases, such as IgA nephropathy.
MeSH term(s)	Animals ; Antibodies, Monoclonal ; Antibody-Dependent Cell Cytotoxicity ; Glomerulonephritis, IGA ; Immunoglobulin A ; Rats ; Receptors, Antigen, B-Cell
Chemical Substances	Antibodies, Monoclonal ; Immunoglobulin A ; Receptors, Antigen, B-Cell
Language	English
Publishing date	2022-06-23
Publishing country	United States
Document type	Journal Article
ISSN	2167-9436
ISSN (online)	2167-9436
DOI	10.1089/mab.2021.0032
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Article: Effects of dietary phosphorus concentration and phosphate salt form on renal tubule function in unilateral nephrectomized rats.

Mori, Kikue / Katsumata, Shin-Ichi / Miyajima, Katsuhiro / Uno, Kinuko / Matsuzaki, Hiroshi

Nutrition and health

2022 , Page(s) 2601060221122223

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-08-29
Publishing country	England
Document type	Journal Article
ZDB-ID	603215-1
ISSN	2047-945X ; 0260-1060
ISSN (online)	2047-945X
ISSN	0260-1060
DOI	10.1177/02601060221122223
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Article: Interaction between glycolipid MPIase and proteinaceous factors during protein integration into the cytoplasmic membrane of

Nishikawa, Hanako / Sawasato, Katsuhiro / Mori, Shoko / Fujikawa, Kohki / Nomura, Kaoru / Shimamoto, Keiko / Nishiyama, Ken-Ichi

Frontiers in molecular biosciences

2022 Volume 9, Page(s) 986602

Abstract: Protein integration into biomembranes is an essential biological phenomenon common to all organisms. While various factors involved in protein integration, such as SRP, SecYEG and YidC, are proteinaceous, we identified a glycolipid named MPIase (Membrane ...

Abstract	Protein integration into biomembranes is an essential biological phenomenon common to all organisms. While various factors involved in protein integration, such as SRP, SecYEG and YidC, are proteinaceous, we identified a glycolipid named MPIase (Membrane Protein Integrase), which is present in the cytoplasmic membrane of
Language	English
Publishing date	2022-08-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2022.986602
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Article ; Online: DNA methylation-based age estimation and quantification of the degradation levels of bisulfite-converted DNA.

Shiga, Mihiro / Asari, Masaru / Takahashi, Yuta / Isozaki, Shotaro / Hoshina, Chisato / Mori, Kanae / Namba, Ryo / Okuda, Katsuhiro / Shimizu, Keiko

Legal medicine (Tokyo, Japan)

2023 Volume 67, Page(s) 102336

Abstract: DNA methylation modifications are known to influence epigenetic phenomena and have been a focus of forensic science research for some time. Degraded DNA after bisulfite treatment is widely used in DNA methylation analysis. In this study, we analyzed ... ...

Abstract	DNA methylation modifications are known to influence epigenetic phenomena and have been a focus of forensic science research for some time. Degraded DNA after bisulfite treatment is widely used in DNA methylation analysis. In this study, we analyzed methylation levels at 12 CpG sites of four selected genomic regions by pyrosequencing after bisulfite treatment. DNA was extracted from buccal swab samples collected from 102 Japanese individuals who were 21-77 years old. We also developed a simple method to quantify the degradation levels of bisulfite-converted DNA by real-time PCR, and evaluated the effect of DNA degradation on age estimation. We found that the methylation levels and chronological ages were highly correlated in the four selected regions, and the mean absolute deviation (MAD) between chronological and estimated ages was low at 3.88 years. These results indicated that pyrosequencing analysis at the 12 CpGs was useful for age estimation in the Japanese population. To develop a sensitive quantification method, we analyzed the amplification efficiency of short and long fragments from 10 regions by real-time PCR. The amplification efficiency was highest for CCDC102B, and the degradation levels of bisulfite-converted DNA for the 102 samples were categorized as moderately or heavily degraded. For the younger age groups (20-49 years), the MADs were lower for moderately degraded DNA than they were for heavily degraded DNA. This finding indicates that degradation levels affected the accuracy of age estimation in most of the samples; the exception was the samples from the 50-77 years age group.
MeSH term(s)	Humans ; Young Adult ; Adult ; Middle Aged ; Aged ; DNA Methylation/genetics ; Aging/genetics ; CpG Islands/genetics ; DNA/genetics ; Forensic Genetics/methods ; Sulfites
Chemical Substances	hydrogen sulfite (OJ9787WBLU) ; DNA (9007-49-2) ; Sulfites
Language	English
Publishing date	2023-10-20
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	2019555-2
ISSN	1873-4162 ; 1344-6223
ISSN (online)	1873-4162
ISSN	1344-6223
DOI	10.1016/j.legalmed.2023.102336
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Article ; Online: Exploration of urine metabolic biomarkers for new-onset, untreated pediatric epilepsy: A gas and liquid chromatography mass spectrometry-based metabolomics study.

Akiyama, Tomoyuki / Saigusa, Daisuke / Inoue, Takushi / Tokorodani, Chiho / Akiyama, Mari / Michiue, Rie / Mori, Atsushi / Hishinuma, Eiji / Matsukawa, Naomi / Shibata, Takashi / Tsuchiya, Hiroki / Kobayashi, Katsuhiro

Brain & development

2024 Volume 46, Issue 4, Page(s) 180–186

Abstract: Objective: The discovery of objective indicators for recent epileptic seizures will help confirm the diagnosis of epilepsy and evaluate therapeutic effects. Past studies had shortcomings such as the inclusion of patients under treatment and those with ... ...

Abstract	Objective: The discovery of objective indicators for recent epileptic seizures will help confirm the diagnosis of epilepsy and evaluate therapeutic effects. Past studies had shortcomings such as the inclusion of patients under treatment and those with various etiologies that could confound the analysis results significantly. We aimed to minimize such confounding effects and to explore the small molecule biomarkers associated with the recent occurrence of epileptic seizures using urine metabolomics. Methods: This is a multicenter prospective study. Subjects included pediatric patients aged 2 to 12 years old with new-onset, untreated epilepsy, who had had the last seizure within 1 month before urine collection. Controls included healthy children aged 2 to 12 years old. Those with underlying or chronic diseases, acute illnesses, or recent administration of medications or supplements were excluded. Targeted metabolome analysis of spot urine samples was conducted using gas chromatography (GC)- and liquid chromatography (LC)-tandem mass spectrometry (MS/MS). Results: We enrolled 17 patients and 21 controls. Among 172 metabolites measured by GC/MS/MS and 41 metabolites measured by LC/MS/MS, only taurine was consistently reduced in the epilepsy group. This finding was subsequently confirmed by the absolute quantification of amino acids. No other metabolites were consistently altered between the two groups. Conclusions: Urine metabolome analysis, which covers a larger number of metabolites than conventional biochemistry analyses, found no consistently altered small molecule metabolites except for reduced taurine in epilepsy patients compared to healthy controls. Further studies with larger samples, subjects with different ages, expanded target metabolites, and the investigation of plasma samples are required.
MeSH term(s)	Humans ; Child ; Child, Preschool ; Tandem Mass Spectrometry ; Liquid Chromatography-Mass Spectrometry ; Prospective Studies ; Metabolome ; Epilepsy/diagnosis ; Seizures ; Taurine ; Biomarkers
Chemical Substances	Taurine (1EQV5MLY3D) ; Biomarkers
Language	English
Publishing date	2024-01-03
Publishing country	Netherlands
Document type	Multicenter Study ; Journal Article
ZDB-ID	604822-5
ISSN	1872-7131 ; 0387-7604
ISSN (online)	1872-7131
ISSN	0387-7604
DOI	10.1016/j.braindev.2023.12.004
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Article ; Online: Brain metastasis in a patient with BRCA2-mutated treatment-related neuroendocrine prostate carcinoma and long-term response to radiotherapy and Olaparib: A case report and literature review.

Uehara, Rio / Obinata, Daisuke / Hashimoto, Sho / Nakahara, Ken / Uchida, Hideaki / Yoshizawa, Tsuyoshi / Mochida, Junichi / Yamaguchi, Kenya / Sakaguchi, Masakuni / Ozawa, Yoshinari / Mori, Fumi / Miura, Katsuhiro / Ishige, Toshiyuki / Masuda, Shinobu / Nakayama, Tomohiro / Takahashi, Satoru

Medicine

2024 Volume 103, Issue 9, Page(s) e37371

Abstract: Background: A new subtype of prostate cancer called treatment-related neuroendocrine prostate carcinoma (t-NEPC) was added to the revised World Health Organization classification of prostate cancer in 2022. t-NEPC cases are increasing, and there is no ... ...

Abstract	Background: A new subtype of prostate cancer called treatment-related neuroendocrine prostate carcinoma (t-NEPC) was added to the revised World Health Organization classification of prostate cancer in 2022. t-NEPC cases are increasing, and there is no established standard treatment. Methods: A 49-year-old male patient was referred to our department for dysuria. A rectal examination and a prostate biopsy revealed stony hardness and prostate adenocarcinoma, respectively. Imaging studies confirmed the presence of multiple bone and lymph node metastases. The patient was started on upfront treatment with androgen deprivation therapy and an androgen receptor signaling inhibitor, which resulted in a significant (>90%) decrease in prostate-specific antigen (PSA) levels. The patient experienced postrenal failure 6 months later, attributable to local disease progression. Concurrently, there was an elevation in neuron-specific enolase (NSE) levels and an enlargement of pelvic lymph node metastases, without PSA progression. Results: Biopsy specimen for cancer genome profiling revealed deletion of BRCA 2 and PTEN, AR amplification, and the presence of the TMPRSS2-ERG fusion gene. Based on increased NSE and BRCA2 mutations, a diagnosis of t-NEPC with BRCA2 mutation was eventually made. The patient received docetaxel chemotherapy and pelvic radiotherapy. Subsequently, he was treated with olaparib. His NSE levels decreased, and he achieved a complete response (CR). However, 18 months following the olaparib administration, brain metastases appeared despite the absence of pelvic tumor relapse, and the patient's PSA levels remained low. Consequently, the patient underwent resection of the brain metastases using gamma knife and whole-brain radiotherapy but died approximately 3 months later. Conclusion subsections: Platinum-based chemotherapy is often administered for the treatment of t-NEPC, but there are few reports on the effectiveness of olaparib in patients with BRCA2 mutations. In a literature review, this case demonstrated the longest duration of effectiveness with olaparib alone without platinum-based chemotherapy. Additionally, the occurrence of relatively rare, fatal brain metastases in prostate cancer after a long period of CR suggests the necessity of regular brain imaging examinations.
MeSH term(s)	Male ; Humans ; Middle Aged ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/therapy ; Prostatic Neoplasms/diagnosis ; Prostate-Specific Antigen ; Androgen Antagonists/therapeutic use ; Prostate/pathology ; Lymphatic Metastasis ; Neoplasm Recurrence, Local/drug therapy ; Brain Neoplasms/therapy ; Brain Neoplasms/drug therapy ; Carcinoma/drug therapy ; BRCA2 Protein ; Phthalazines ; Piperazines
Chemical Substances	Prostate-Specific Antigen (EC 3.4.21.77) ; olaparib (WOH1JD9AR8) ; Androgen Antagonists ; BRCA2 protein, human ; BRCA2 Protein ; Phthalazines ; Piperazines
Language	English
Publishing date	2024-02-28
Publishing country	United States
Document type	Review ; Case Reports ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000037371
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Article ; Online: Multidirectional analysis for a colchicine poisoning case revealed detail cause of death and its mechanism.

Okuda, Katsuhiro / Isozaki, Shotaro / Asari, Masaru / Tanaka, Hiroki / Horioka, Kie / Takahashi, Yuta / Hoshina, Chisato / Yamada, Hiromi / Mori, Kanae / Namba, Ryo / Shiono, Hiroshi / Ogawa, Katsuhiro / Shimizu, Keiko

Legal medicine (Tokyo, Japan)

2022 Volume 58, Page(s) 102092

Abstract: The appearance of Meadow saffron (Colchicum autumnale), which contains colchicine, closely resembles Alpine leek (Allium victorialis), a popular edible wild vegetable in Northern Japan. This often results in the accidental ingestion of Meadow saffron and ...

Abstract	The appearance of Meadow saffron (Colchicum autumnale), which contains colchicine, closely resembles Alpine leek (Allium victorialis), a popular edible wild vegetable in Northern Japan. This often results in the accidental ingestion of Meadow saffron and acute colchicine poisoning deaths. Here, we report on a case of acute colchicine poisoning death caused by the accidental ingestion of Meadow saffron. A man in his 70 s had been given wild vegetables from his neighborhood, which were then cooked and eaten by himself and his wife. Several hours later, they suffered from abdominal pain, vomiting, and diarrhea. They immediately went to the hospital and received routine treatment. While his wife made a full recovery, he died at home two days after consumption of the vegetables. A forensic autopsy was conducted five days after ingestion of the Meadow saffron and a lethal concentration (21.5 ng/mL) of colchicine in the peripheral blood sample was detected by liquid chromatography-tandem mass spectrometry. Distribution of colchicine in body fluids, tissues and gastrointestinal contents was also investigated. Some of the plants he had eaten were identified as Alpine leek or Meadow saffron by genetic analysis of his stomach contents. Histopathological examination showed apoptotic cells and cell cycle arrest at the metaphase in the intestinal crypts and testis. In addition, we detected high concentrations of endotoxins and tumor necrosis factor-α in his blood, indicating that intestinal mucosal injury induced by colchicine poisoning had allowed endotoxins to invade the body, causing death by endotoxin shock.
MeSH term(s)	Cause of Death ; Colchicine ; Colchicum ; Endotoxins/adverse effects ; Humans ; Male ; Vomiting/chemically induced
Chemical Substances	Endotoxins ; Colchicine (SML2Y3J35T)
Language	English
Publishing date	2022-05-23
Publishing country	Ireland
Document type	Case Reports ; Journal Article
ZDB-ID	2019555-2
ISSN	1873-4162 ; 1344-6223
ISSN (online)	1873-4162
ISSN	1344-6223
DOI	10.1016/j.legalmed.2022.102092
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