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  1. Article ; Online: Non-lesional status epilepticus in a patient with coronavirus disease 2019.

    Balloy, Gaëlle / Leclair-Visonneau, Laurène / Péréon, Yann / Magot, Armelle / Peyre, Arnaud / Mahé, Pierre-Joachim / Derkinderen, Pascal

    Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology

    2020  Volume 131, Issue 8, Page(s) 2059–2061

    MeSH term(s) Brain Waves ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Humans ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnosis ; Status Epilepticus/complications ; Status Epilepticus/diagnosis
    Keywords covid19
    Language English
    Publishing date 2020-05-13
    Publishing country Netherlands
    Document type Case Reports ; Letter
    ZDB-ID 1463630-x
    ISSN 1872-8952 ; 0921-884X ; 1388-2457
    ISSN (online) 1872-8952
    ISSN 0921-884X ; 1388-2457
    DOI 10.1016/j.clinph.2020.05.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ui II Zs.140: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Optimizing Western Blots for the Detection of Endogenous α-Synuclein in the Enteric Nervous System.

    Preterre, Cécile / Corbillé, Anne-Gaëlle / Balloy, Gaëlle / Letournel, Franck / Neunlist, Michel / Derkinderen, Pascal

    Journal of Parkinson's disease

    2015  Volume 5, Issue 4, Page(s) 765–772

    Abstract: Background: Alpha-synuclein containing inclusions in neurons, the characteristic pathological lesions of Parkinson's disease (PD), are not limited to the central nervous system, but also affect the enteric nervous system (ENS). This suggests that the ... ...

    Abstract Background: Alpha-synuclein containing inclusions in neurons, the characteristic pathological lesions of Parkinson's disease (PD), are not limited to the central nervous system, but also affect the enteric nervous system (ENS). This suggests that the ENS offer some potential as a surrogate of central nervous system pathology and that it may represent an original source of biomarkers for PD. However, the usefulness of α-synuclein detection in gastrointestinal biopsies as a biomarker for PD is still unclear, as the different immunohistochemical methods employed to date have led to conflicting results.
    Objective: Our aim is to propose an optimized immunoblotting method for the detection of endogenous α-synuclein in the healthy ENS that may be used to supplement the immunohistochemical analysis.
    Methods: Primary culture of rat ENS and homogenates of human small intestine were analyzed by Western Blot using seven different α-synuclein and phospho-α-synuclein antibodies along with two methods that increase α-synuclein retention on blot membranes, namely incubation of the membranes with paraformaldehyde (PFA) or treatment of samples with the crosslinker dithiobis[succinimidylpropionate] (DSP).
    Results: A moderate improvement in the detection of endogenous enteric α-synuclein was observed following membrane fixation with PFA for only two of the seven antibodies we tested. Immunodetection of total and phosphorylated α-synuclein in the ENS was markedly improved when samples were treated with DSP, regardless of the antibody used.
    Conclusions: Our results demonstrate that the detection of α-synuclein in the gut by Western Blot can be optimized by using methods for enhanced membrane retention of the protein along with the appropriate antibody. Such an optimized protocol opens the way to the development of novel biomarkers for PD that will enable a quantification of α-synuclein in gastrointestinal biopsies.
    MeSH term(s) Animals ; Biomarkers/metabolism ; Blotting, Western/methods ; Blotting, Western/standards ; Enteric Nervous System/metabolism ; Humans ; Intestine, Small/innervation ; Intestine, Small/metabolism ; Parkinson Disease/diagnosis ; Rats ; alpha-Synuclein/analysis
    Chemical Substances Biomarkers ; SNCA protein, human ; Snca protein, rat ; alpha-Synuclein
    Language English
    Publishing date 2015
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2620609-2
    ISSN 1877-718X ; 1877-7171
    ISSN (online) 1877-718X
    ISSN 1877-7171
    DOI 10.3233/JPD-150670
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6964: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  3. Article: Non-lesional status epilepticus in a patient with coronavirus disease 2019

    Balloy, Gaëlle / Leclair-Visonneau, Laurène / Péréon, Yann / Magot, Armelle / Peyre, Arnaud / Mahé, Pierre-Joachim / Derkinderen, Pascal

    Clin Neurophysiol

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #245175
    Database COVID19

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