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Article ; Online: Application of a distinctly bent, trinuclear, end-to-end azide bridged, mixed valence cobalt(iii/ii/iii) complex in the fabrication of photosensitive Schottky barrier diodes.

Bhunia, Sudip / Das, Mainak / Banerjee, Snehasis / Drew, Michael G B / Ray, Partha Pratim / Chattopadhyay, Shouvik

RSC advances

2024 Volume 14, Issue 16, Page(s) 11185–11196

Abstract: A mixed-valence trinuclear cobalt(iii)-cobalt(ii)-cobalt(iii) complex, [(μ-1,3- ... ...

Abstract	A mixed-valence trinuclear cobalt(iii)-cobalt(ii)-cobalt(iii) complex, [(μ-1,3-N
Language	English
Publishing date	2024-04-08
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/d4ra01406e
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Article ; Online: Cell-specific housekeeping role of lncRNAs in COVID-19-infected and recovered patients.

Chattopadhyay, Partha / Mehta, Priyanka / Soni, Jyoti / Tardalkar, Kishore / Joshi, Meghnad / Pandey, Rajesh

NAR genomics and bioinformatics

2024 Volume 6, Issue 1, Page(s) lqae023

Abstract: A plethora of studies have demonstrated the roles of lncRNAs in modulating disease severity and outcomes during infection. However, the spatio-temporal expression of these lncRNAs is poorly understood. In this study, we used single-cell RNA-seq to ... ...

Abstract	A plethora of studies have demonstrated the roles of lncRNAs in modulating disease severity and outcomes during infection. However, the spatio-temporal expression of these lncRNAs is poorly understood. In this study, we used single-cell RNA-seq to understand the spatio-temporal expression dynamics of lncRNAs across healthy, SARS-CoV-2-infected, and recovered individuals and their functional role in modulating the disease and recovery. We identified 203 differentially expressed lncRNAs, including cell type-specific ones like
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Journal Article
ISSN	2631-9268
ISSN (online)	2631-9268
DOI	10.1093/nargab/lqae023
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Article: Dynamics of Whole Transcriptome Analysis (WTA) and Surface markers expression (AbSeq) in Immune Cells of COVID-19 Patients and Recovered captured through Single Cell Genomics.

Soni, Jyoti / Chattopadhyay, Partha / Mehta, Priyanka / Mohite, Ramakant / Tardalkar, Kishore / Joshi, Meghnad / Pandey, Rajesh

Frontiers in medicine

2024 Volume 11, Page(s) 1297001

Abstract: Introduction: Single-cell multi-omics studies, such as multidimensional transcriptomics (whole transcriptomic analysis, WTA), and surface marker analysis (antibody sequencing, AbSeq), have turned out to be valuable techniques that offer inaccessible ... ...

Abstract	Introduction: Single-cell multi-omics studies, such as multidimensional transcriptomics (whole transcriptomic analysis, WTA), and surface marker analysis (antibody sequencing, AbSeq), have turned out to be valuable techniques that offer inaccessible possibilities for single-cell profiling of mRNA, lncRNA, and proteins. Methods: We used this technique to understand the dynamics of mRNA and protein-level differences in healthy, COVID-19-infected and recovered individuals using peripheral blood mononuclear cells (PBMCs). Our results demonstrate that compared to mRNA expression, protein abundance is a better indicator of the disease state. Results: We demonstrate that compared to mRNA expression, protein abundance is a better indicator of the disease state. We observed high levels of cell identity and regulatory markers, Discussion: Furthermore, our study emphasizes the significance of employing an oligo-based method (AbSeq) that can help in diagnosis, prognosis, and protection from disease/s by identifying cell surface markers that are unique to different cell types or states. It also allows simultaneous study of a vast array of markers, surpassing the constraints of techniques like FACS to query the vast repertoire of proteins.
Language	English
Publishing date	2024-01-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2024.1297001
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Article ; Online: Liquified calcinosis cutis masquerading as an abscess in juvenile dermatomyositis.

Dey, Sonali / Bhowmick, Kaustav / Chattopadhyay, Arghya / Sinhamahapatra, Pradyot / Mitra, Kaustav / Ghosh, Biswadip / Ghorai, Partha

Joint bone spine

2023 Volume 90, Issue 5, Page(s) 105565

MeSH term(s)	Humans ; Dermatomyositis/complications ; Dermatomyositis/diagnosis ; Calcinosis Cutis ; Abscess ; Calcinosis/diagnostic imaging ; Skin Diseases
Language	English
Publishing date	2023-03-18
Publishing country	France
Document type	Journal Article
ZDB-ID	2020487-5
ISSN	1778-7254 ; 1297-319X
ISSN (online)	1778-7254
ISSN	1297-319X
DOI	10.1016/j.jbspin.2023.105565
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Article ; Online: Effect of short-term use of FFP2 (N95) masks on the salivary metabolome of young healthy volunteers: a pilot study.

Islam, Sk Ramiz / Prusty, Debasish / Maiti, Subhadip / Dutta, Raju / Chattopadhyay, Partha / Manna, Soumen Kanti

Molecular omics

2023 Volume 19, Issue 5, Page(s) 383–394

Abstract: The use of face masks has become an integral part of public life in the post-pandemic era. However, the understanding of the effect of wearing masks on physiology remains incomplete and is required for informing public health policies. For the first time, ...

Abstract	The use of face masks has become an integral part of public life in the post-pandemic era. However, the understanding of the effect of wearing masks on physiology remains incomplete and is required for informing public health policies. For the first time, we report the effects of wearing FFP2 masks on the metabolic composition of saliva, a proximal matrix to breath, along with cardiopulmonary parameters. Un-induced saliva was collected from young (31.2 ± 6.3 years) healthy volunteers (
MeSH term(s)	Humans ; COVID-19 ; SARS-CoV-2 ; Masks ; Pilot Projects ; Metabolome
Language	English
Publishing date	2023-06-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2515-4184
ISSN (online)	2515-4184
DOI	10.1039/d2mo00232a
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Article: Single-Cell Genomics: Enabling the Functional Elucidation of Infectious Diseases in Multi-Cell Genomes.

Sahni, Shweta / Chattopadhyay, Partha / Khare, Kriti / Pandey, Rajesh

Pathogens (Basel, Switzerland)

2021 Volume 10, Issue 11

Abstract: Since the time when detection of gene expression in single cells by microarrays to the Next Generation Sequencing (NGS) enabled Single Cell Genomics (SCG), it has played a pivotal role to understand and elucidate the functional role of cellular ... ...

Abstract	Since the time when detection of gene expression in single cells by microarrays to the Next Generation Sequencing (NGS) enabled Single Cell Genomics (SCG), it has played a pivotal role to understand and elucidate the functional role of cellular heterogeneity. Along this journey to becoming a key player in the capture of the individuality of cells, SCG overcame many milestones, including scale, speed, sensitivity and sample costs (4S). There have been many important experimental and computational innovations in the efficient analysis and interpretation of SCG data. The increasing role of AI in SCG data analysis has further enhanced its applicability in building models for clinical intervention. Furthermore, SCG has been instrumental in the delineation of the role of cellular heterogeneity in specific diseases, including cancer and infectious diseases. The understanding of the role of differential immune responses in driving coronavirus disease-2019 (COVID-19) disease severity and clinical outcomes has been greatly aided by SCG. With many variants of concern (VOC) in sight, it would be of great importance to further understand the immune response specificity
Language	English
Publishing date	2021-11-12
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10111467
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Article ; Online: SARS-CoV-2 infection severity and mortality is modulated by repeat-mediated regulation of alternative splicing.

Mehta, Priyanka / Chattopadhyay, Partha / Ravi, Varsha / Tarai, Bansidhar / Budhiraja, Sandeep / Pandey, Rajesh

Microbiology spectrum

2023 , Page(s) e0135123

Abstract: Like single-stranded RNA viruses, SARS-CoV-2 hijacks the host transcriptional machinery for its own replication. Numerous traditional differential gene expression-based investigations have examined the diverse clinical symptoms caused by SARS-CoV-2 ... ...

Abstract	Like single-stranded RNA viruses, SARS-CoV-2 hijacks the host transcriptional machinery for its own replication. Numerous traditional differential gene expression-based investigations have examined the diverse clinical symptoms caused by SARS-CoV-2 infection. The virus, on the other hand, also affects the host splicing machinery, causing host transcriptional dysregulation, which can lead to diverse clinical outcomes. Hence, in this study, we performed host transcriptome sequencing of 125 hospital-admitted COVID-19 patients to understand the transcriptomic differences between the severity sub-phenotypes of mild, moderate, severe, and mortality. We performed transcript-level differential expression analysis, investigated differential isoform usage, looked at the splicing patterns within the differentially expressed transcripts (DET), and elucidated the possible genome regulatory features. Our DTE analysis showed evidence of diminished transcript length and diversity as well as altered promoter site usage in the differentially expressed protein-coding transcripts in the COVID-19 mortality patients. We also investigated the potential mechanisms driving the alternate splicing and discovered a compelling differential enrichment of repeats in the promoter region and a specific enrichment of SINE (Alu) near the splicing sites of differentially expressed transcripts. These findings suggested a repeat-mediated plausible regulation of alternative splicing as a potential modulator of COVID-19 disease severity. In this work, we emphasize the role of scarcely elucidated functional role of alternative splicing in influencing COVID-19 disease severity sub-phenotypes, clinical outcomes, and its putative mechanism. IMPORTANCE The wide range of clinical symptoms reported during the COVID-19 pandemic inherently highlights the numerous factors that influence the progression and prognosis of SARS-CoV-2 infection. While several studies have investigated the host response and discovered immunological dysregulation during severe infection, most of them have the common theme of focusing only up to the gene level. Viruses, especially RNA viruses, are renowned for hijacking the host splicing machinery for their own proliferation, which inadvertently puts pressure on the host transcriptome, exposing another side of the host response to the pathogen challenge. Therefore, in this study, we examine host response at the transcript-level to discover a transcriptional difference that culminates in differential gene-level expression. Importantly, this study highlights diminished transcript diversity and possible regulation of transcription by differentially abundant repeat elements near the promoter region and splicing sites in COVID-19 mortality patients, which together with differentially expressed isoforms hold the potential to elaborate disease severity and outcome.
Language	English
Publishing date	2023-08-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.01351-23
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Article ; Online: Study of the human liver by ultrasound examination to search for variations of venous anatomy.

Ain, Partha Sarathi / Roy, Uttam Kumar / Meyur, Rudradev / Chattopadhyay, Subhajit

Folia medica

2022 Volume 63, Issue 5, Page(s) 676–685

Abstract: Introduction: Knowledge about venous patterns (especially portal and hepatic venous) and their variations are extremely crucial for liver transplantation and other surgical procedures on liver. Studies have been done at national or international level ... ...

Abstract	Introduction: Knowledge about venous patterns (especially portal and hepatic venous) and their variations are extremely crucial for liver transplantation and other surgical procedures on liver. Studies have been done at national or international level on variations of hepatic venous patterns by CT or MRI or by dissection on cadaveric liver. No definitive or authentic study on this topic could be noted in electronic and print media, as well as in standard textbook. Considering low cost and noninvasive nature, we utilized ultrasonography to bridge this lacuna. Aim: To estimate prevalence and types of anatomical variations of hepatic venous system and to determine association of among variations. Materials and methods: It is an observational cross-sectional study where ultrasonographic evaluation of hepatic venous pattern on adult population of either sex (consecutive sampling) without any major liver disorders was done in RG Kar Medical College and data were analyzed with appropriate statistical procedures. Results: Normal hepatic venous pattern along with normal portal venous pattern is much higher in our study, which is a unique finding. The proportion of normal portal venous pattern is more in extra hepatic bifurcation group. Variations of portal vein and normal hepatic venous pattern are more in case of intrahepatic bifurcation of portal venous branching group. Overall presence of intrahepatic bifurcation of portal vein is significantly higher than extrahepatic bifurcation. Conclusions: This study provides an updated database for the prevalence and distribution of anatomic variations of the hepatic venous and portal venous system.
MeSH term(s)	Adult ; Cross-Sectional Studies ; Hepatic Veins/anatomy & histology ; Hepatic Veins/diagnostic imaging ; Humans ; Liver/diagnostic imaging ; Portal Vein/anatomy & histology ; Portal Vein/diagnostic imaging ; Portal Vein/surgery ; Ultrasonography
Language	English
Publishing date	2022-07-18
Publishing country	Bulgaria
Document type	Journal Article ; Observational Study
ZDB-ID	300275-5
ISSN	1314-2143 ; 0430-8638 ; 0204-8043
ISSN (online)	1314-2143
ISSN	0430-8638 ; 0204-8043
DOI	10.3897/folmed.63.e60772
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Zs.A 972: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Noncoding RNAs: modulators and modulatable players during infection-induced stress response.

Chattopadhyay, Partha / Srinivasa Vasudevan, Janani / Pandey, Rajesh

Briefings in functional genomics

2021 Volume 20, Issue 1, Page(s) 28–41

Abstract: The human genome has an almost equal distribution of unique and transposable genetic elements. Although at the transcriptome level, a relatively higher contribution from transposable elements derived RNA has been reported. This is further highlighted ... ...

Abstract	The human genome has an almost equal distribution of unique and transposable genetic elements. Although at the transcriptome level, a relatively higher contribution from transposable elements derived RNA has been reported. This is further highlighted with evidence from pervasive transcription. Of the total RNA, noncoding RNAs (ncRNAs) are significant contributors to the transcriptome pool with sizeable fraction from repetitive elements of the human genome, inclusive of Long Interspersed Nuclear Elements (LINEs) and Short Interspersed Nuclear Elements (SINEs). ncRNAs are increasingly being implicated in diverse functional roles especially during conditions of stress. These stress responses are driven through diverse mediators, inclusive of long and short ncRNAs. ncRNAs such as MALAT1, GAS5, miR-204 and miR-199a-5p have been functionally involved during oxidative stress, endoplasmic reticulum (ER) stress and unfolded protein response (UPR). Also, within SINEs, Alu RNAs derived from primate-specific Alu repeats with ~11% human genome contribution, playing a significant role. Pathogenic diseases, including the recent COVID-19, leads to differential regulation of ncRNAs. Although, limited evidence suggests the need for an inquest into the role of ncRNAs in determining the host response towards pathogen challenge.
MeSH term(s)	COVID-19/genetics ; COVID-19/virology ; Cytokines/physiology ; Endoplasmic Reticulum Stress ; Host-Pathogen Interactions ; Humans ; Infections/genetics ; Infections/metabolism ; Long Interspersed Nucleotide Elements ; Oxidative Stress ; RNA, Untranslated/genetics ; RNA, Untranslated/physiology ; SARS-CoV-2/isolation & purification ; Short Interspersed Nucleotide Elements ; Unfolded Protein Response
Chemical Substances	Cytokines ; RNA, Untranslated
Language	English
Publishing date	2021-01-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2540916-5
ISSN	2041-2657 ; 2041-2649 ; 2041-2647
ISSN (online)	2041-2657
ISSN	2041-2649 ; 2041-2647
DOI	10.1093/bfgp/elaa026
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Zs.A 6076: Show issues

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Article: Drawbar performance of a power tiller on a sandy loam soil of the Nadia district of West Bengal

Hensh, Sujit / Chattopadhyay, Partha Sarathi / Das, Khokan

Research in Agricultural Engineering. , v. 68, no. 1

2022

Abstract: A 9.69 kW power tiller's drawbar performance was tested by using a drawbar loading vehicle consisting of a power tiller with a mould board (MB) plough. A spring-loaded dynamometer was attached between the tested power tiller and the loading vehicle to ... ...

Abstract	A 9.69 kW power tiller's drawbar performance was tested by using a drawbar loading vehicle consisting of a power tiller with a mould board (MB) plough. A spring-loaded dynamometer was attached between the tested power tiller and the loading vehicle to measure the drawbar pull. The drawbar pull was changed from 0.905 kN to 2.232 kN by varying the operating depth of the MB plough. Empirical equations were developed to correlate the drawbar pull to the wheel slip, drawbar power, fuel consumption, and drawbar specific fuel consumption (DBSFC), and one was developed to correlate the drawbar power to the wheel slip. The wheel slip increased exponentially with an increase in the drawbar pull and drawbar power. A maximum wheel slippage of 48.94% was observed at a 2.232 kN drawbar pull and 0.763 kW drawbar power. A second-degree polynomial equation was found to correlate the drawbar pull to the drawbar power, fuel consumption, and DBSFC. The maximum drawbar power was found as 0.763 kW at a 2.02 kN drawbar pull, which was 7.87% of the rated engine power. The fuel consumption increased by 66.93%, and the DBSFC reduced by 10.56% due to the increase of the drawbar pull from 0.905 kN to 2.232 kN. The lowest DBSFC of 2.01 kg·kWh<sup>–1</sup> was found at a 2.232 kN drawbar pull.
Keywords	energy use and consumption ; equations ; research ; sandy loam soils ; India
Language	English
Dates of publication	2022-0323
Size	p. 41-46.
Publishing place	Czech Academy of Agricultural Sciences
Document type	Article
ZDB-ID	2012829-0
ISSN	1212-9151
ISSN	1212-9151
DOI	10.17221/16/2021-rae
Database	NAL-Catalogue (AGRICOLA)

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