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  1. Article ; Online: Characterization of Aldosterone-producing Cell Cluster (APCC) at Single-cell Resolution.

    Iwahashi, Norifusa / Umakoshi, Hironobu / Seki, Tsugio / Gomez-Sanchez, Celso E / Mukai, Kuniaki / Suematsu, Makoto / Umezawa, Yuta / Oya, Mototsugu / Kosaka, Takeo / Seki, Masahide / Suzuki, Yutaka / Horiuchi, Yutaka / Ogawa, Yoshihiro / Nishimoto, Koshiro

    The Journal of clinical endocrinology and metabolism

    2022  Volume 107, Issue 9, Page(s) 2439–2448

    Abstract: Context: The adrenal cortex consists of zona glomerulosa (ZG), fasciculata (ZF), and reticularis. Aldosterone-producing cell clusters (APCCs) that strongly express aldosterone synthase (CYP11B2) are frequently found in adult adrenals and harbor somatic ... ...

    Abstract Context: The adrenal cortex consists of zona glomerulosa (ZG), fasciculata (ZF), and reticularis. Aldosterone-producing cell clusters (APCCs) that strongly express aldosterone synthase (CYP11B2) are frequently found in adult adrenals and harbor somatic mutations that are also detected in aldosterone-producing adenomas (APAs). Primary aldosteronism is mainly caused by APAs or idiopathic hyperaldosteronism (IHA). We presume that APCCs are causing IHA and are precursors of APAs. However, the gene expression characteristics and especially the development of APCCs are not well understood.
    Objective: This study aimed to analyze the transcriptome of APCCs at single-cell resolution and infer the developmental trajectory.
    Methods: Single-cell RNA sequencing (scRNA-seq) of 2 adult adrenals was performed.
    Results: Immunohistochemical analyses confirmed the 2 adrenals had APCCs. scRNA-seq data of 2928 adrenal cells were obtained and 1765 adrenocortical cells were identified based on unsupervised clustering and the marker gene expression. The adrenocortical cells were divided into 6 clusters, of which 3 clusters (923 cells) were composed of APCC/ZG cells. By further subclustering, the APCC/ZG cells were divided into 3 clusters (clusters C1, C2, and C3), we finally identified APCC cluster (C3) and ZG cluster (C1). Cluster C2 seemed to be ZG-to-ZF transitional cells. RNA velocity analysis inferred the developmental direction from cluster ZG-cluster-C1 to APCC-cluster-C3. The scRNA-seq additionally revealed that many CYP11B2-positive cells were positive for CYP11B1 and/or CYP17A1, which were essential for cortisol but not for aldosterone production.
    Conclusions: Our results revealed the gene expression characteristics of APCC at single-cell resolution and show that some ZG cells remodel to APCC.
    MeSH term(s) Adenoma/metabolism ; Adrenal Cortex Neoplasms/metabolism ; Adrenal Glands/metabolism ; Adult ; Aldosterone/metabolism ; Cytochrome P-450 CYP11B2/genetics ; Cytochrome P-450 CYP11B2/metabolism ; Humans ; Hyperaldosteronism/metabolism ; Zona Glomerulosa/metabolism
    Chemical Substances Aldosterone (4964P6T9RB) ; Cytochrome P-450 CYP11B2 (EC 1.14.15.4)
    Language English
    Publishing date 2022-07-16
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 3029-6
    ISSN 1945-7197 ; 0021-972X
    ISSN (online) 1945-7197
    ISSN 0021-972X
    DOI 10.1210/clinem/dgac394
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  2. Article ; Online: Familial Hyperaldosteronism Type 3 with a Rapidly Growing Adrenal Tumor: An In Situ Aldosterone Imaging Study.

    Takizawa, Nae / Tanaka, Susumu / Nishimoto, Koshiro / Sugiura, Yuki / Suematsu, Makoto / Ohe, Chisato / Ohsugi, Haruyuki / Mizuno, Yosuke / Mukai, Kuniaki / Seki, Tsugio / Oki, Kenji / Gomez-Sanchez, Celso E / Matsuda, Tadashi

    Current issues in molecular biology

    2021  Volume 44, Issue 1, Page(s) 128–138

    Abstract: Primary aldosteronism is most often caused by aldosterone-producing adenoma (APA) and bi-lateral adrenal hyperplasia. Most APAs are caused by somatic mutations of various ion channels and pumps, the most common being the inward-rectifying potassium ... ...

    Abstract Primary aldosteronism is most often caused by aldosterone-producing adenoma (APA) and bi-lateral adrenal hyperplasia. Most APAs are caused by somatic mutations of various ion channels and pumps, the most common being the inward-rectifying potassium channel
    Language English
    Publishing date 2021-12-28
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2000024-8
    ISSN 1467-3045 ; 1467-3037
    ISSN (online) 1467-3045
    ISSN 1467-3037
    DOI 10.3390/cimb44010010
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  3. Article ; Online: Familial Hyperaldosteronism Type 3 with a Rapidly Growing Adrenal Tumor

    Nae Takizawa / Susumu Tanaka / Koshiro Nishimoto / Yuki Sugiura / Makoto Suematsu / Chisato Ohe / Haruyuki Ohsugi / Yosuke Mizuno / Kuniaki Mukai / Tsugio Seki / Kenji Oki / Celso E. Gomez-Sanchez / Tadashi Matsuda

    Current Issues in Molecular Biology, Vol 44, Iss 10, Pp 128-

    An In Situ Aldosterone Imaging Study

    2022  Volume 138

    Abstract: Primary aldosteronism is most often caused by aldosterone-producing adenoma (APA) and bi-lateral adrenal hyperplasia. Most APAs are caused by somatic mutations of various ion channels and pumps, the most common being the inward-rectifying potassium ... ...

    Abstract Primary aldosteronism is most often caused by aldosterone-producing adenoma (APA) and bi-lateral adrenal hyperplasia. Most APAs are caused by somatic mutations of various ion channels and pumps, the most common being the inward-rectifying potassium channel KCNJ5 . Germ line mutations of KCNJ5 cause familial hyperaldosteronism type 3 (FH3), which is associated with severe hyperaldosteronism and hypertension. We present an unusual case of FH3 in a young woman, first diagnosed with primary aldosteronism at the age of 6 years, with bilateral adrenal hyperplasia, who underwent unilateral adrenalectomy (left adrenal) to alleviate hyperaldosteronism. However, her hyperaldosteronism persisted. At the age of 26 years, tomography of the remaining adrenal revealed two different adrenal tumors, one of which grew substantially in 4 months; therefore, the adrenal gland was removed. A comprehensive histological, immunohistochemical, and molecular evaluation of various sections of the adrenal gland and in situ visualization of aldosterone, using matrix-assisted laser desorption/ionization imaging mass spectrometry, was performed. Aldosterone synthase (CYP11B2) immunoreactivity was observed in the tumors and adrenal gland. The larger tumor also harbored a somatic β-catenin activating mutation. Aldosterone visualized in situ was only found in the subcapsular regions of the adrenal and not in the tumors. Collectively, this case of FH3 presented unusual tumor development and histological/molecular findings.
    Keywords familial hyperaldosteronism type 3 ; KCNJ5 ; adrenal tumor ; β-catenin ; MALDI-IMS ; CYP11B2 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Diverse pathological lesions of primary aldosteronism and their clinical significance.

    Nishimoto, Koshiro / Umakoshi, Hironobu / Seki, Tsugio / Yasuda, Masanori / Araki, Ryuichiro / Otsuki, Michio / Katabami, Takuyuki / Shibata, Hirotaka / Ogawa, Yoshihiro / Wada, Norio / Sone, Masakatsu / Okamura, Shintaro / Izawa, Shoichiro / Miyauchi, Shozo / Yoshimoto, Takanobu / Tsuiki, Mika / Naruse, Mitsuhide

    Hypertension research : official journal of the Japanese Society of Hypertension

    2021  Volume 44, Issue 5, Page(s) 498–507

    Abstract: Primary aldosteronism (PA) is mainly clinically classified as unilateral aldosterone-producing adenoma (APA) or bilateral idiopathic hyperaldosteronism. Immunohistochemistry for aldosterone synthase reveals a diverse PA pathology, including pathological ... ...

    Abstract Primary aldosteronism (PA) is mainly clinically classified as unilateral aldosterone-producing adenoma (APA) or bilateral idiopathic hyperaldosteronism. Immunohistochemistry for aldosterone synthase reveals a diverse PA pathology, including pathological APA and aldosterone-producing cell clusters. The relationship between PA pathology and adrenalectomy outcomes was examined herein. Data from 219 unilaterally adrenalectomized PA cases were analyzed. Pathological analyses revealed diverse putative aldosterone-producing lesions. Postoperative biochemical outcomes in 114 cases (test cohort) were classified as complete success (n = 85), partial success (n = 19), and absent success (n = 10). Outcomes in the large and small PA lesion groups, rather than between PA lesion types, were compared at five threshold values for PA lesion sizes (2-6 mm with 1-mm increments) to streamline the results. The proportion of complete success was significantly higher in the large PA lesion group than in the small PA lesion group at the 5-mm threshold only. The proportion of absent success was significantly higher in the small PA lesion group than in the large PA lesion group at all thresholds. Univariate and multivariate analyses of the test cohort identified serum K as an independent predictive factor for the small PA lesion group, which was confirmed in the 105-case validation cohort. Chi-squared automatic interaction detector analysis revealed that the best threshold of serum K for predicting large PA lesions was 2.82 mEq/L. These results will be beneficial for treating PA in clinical settings because patients with low serum K levels and apparent adrenal masses on CT may be subjected to adrenalectomy even if the adrenal venous sampling test is unavailable.
    MeSH term(s) Humans ; Hyperaldosteronism/pathology ; Hyperaldosteronism/therapy
    Language English
    Publishing date 2021-01-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1175297-x
    ISSN 1348-4214 ; 0916-9636
    ISSN (online) 1348-4214
    ISSN 0916-9636
    DOI 10.1038/s41440-020-00579-w
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    Zs.A 3898: Show issues Location:
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  5. Article ; Online: Sodium deficiency regulates rat adrenal zona glomerulosa gene expression.

    Nishimoto, Koshiro / Harris, Ruth B S / Rainey, William E / Seki, Tsugio

    Endocrinology

    2014  Volume 155, Issue 4, Page(s) 1363–1372

    Abstract: Aldosterone is the primary adrenocortical hormone regulating sodium retention, and its production is under the control of the renin-angiotensin-aldosterone system (RAAS). In vitro, angiotensin II can induce aldosterone production in adrenocortical cells ... ...

    Abstract Aldosterone is the primary adrenocortical hormone regulating sodium retention, and its production is under the control of the renin-angiotensin-aldosterone system (RAAS). In vitro, angiotensin II can induce aldosterone production in adrenocortical cells without causing cell proliferation. In vivo, a low-sodium diet activates the RAAS and aldosterone production, at least in part, through an expansion of the adrenal zona glomerulosa (zG) layer. Although these mechanisms have been investigated, RAAS effects on zG gene expression have not been fully elucidated. In this study, we took an unbiased approach to define the complete list of zG transcripts involved in RAAS activation. Adrenal glands were collected from 11-week old Sprague-Dawley rats fed either sodium-deficient (SDef), normal sodium (NS), or high-sodium (HS) diet for 72 hours, and laser-captured zG RNA was analyzed on microarrays containing 27 342 probe sets. When the SDef transcriptome was compared with NS transcriptome (SDef/NS comparison), only 79 and 10 probe sets were found to be up- and down-regulated more than two-fold in SDef, respectively. In SDef/HS comparison, 201 and 68 probe sets were up- and down-regulated in SDef, respectively. Upon gene ontology (GO) analysis of these gene sets, we identified three groups of functionally related GO terms: cell proliferation-associated (group 1), response to stimulus-associated (group 2), and cholesterol/steroid metabolism-associated (group 3) GO terms. Although genes in group 1 may play a critical role in zG layer expansion, those in groups 2 and 3 may have important functions in aldosterone production, and further investigations on these genes are warranted.
    MeSH term(s) Adrenal Glands/metabolism ; Aldosterone/metabolism ; Animals ; Cholesterol/chemistry ; Diet, Sodium-Restricted ; Gene Expression Regulation ; Male ; Oligonucleotide Array Sequence Analysis ; Rats ; Rats, Sprague-Dawley ; Sodium/deficiency ; Sodium Chloride, Dietary ; Steroids/chemistry ; Time Factors ; Transcriptome ; Zona Glomerulosa/metabolism
    Chemical Substances Sodium Chloride, Dietary ; Steroids ; Aldosterone (4964P6T9RB) ; Cholesterol (97C5T2UQ7J) ; Sodium (9NEZ333N27)
    Language English
    Publishing date 2014-01-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 427856-2
    ISSN 1945-7170 ; 0013-7227
    ISSN (online) 1945-7170
    ISSN 0013-7227
    DOI 10.1210/en.2013-1999
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    Uh III Zs.72: Show issues Location:
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  6. Article ; Online: A Novel Method: Super-selective Adrenal Venous Sampling.

    Makita, Kohzoh / Nishimoto, Koshiro / Kiriyama-Kitamoto, Kanako / Karashima, Shigehiro / Seki, Tsugio / Yasuda, Masanori / Matsui, Seishi / Omura, Masao / Nishikawa, Tetsuo

    Journal of visualized experiments : JoVE

    2017  , Issue 127

    Abstract: Primary aldosteronism (PA) and subclinical Cushing's syndrome (SCS) are conditions in which the adrenal glands autonomously produce excessive amounts of aldosterone and cortisol, respectively. The conventional adrenal venous sampling (cAVS) method ... ...

    Abstract Primary aldosteronism (PA) and subclinical Cushing's syndrome (SCS) are conditions in which the adrenal glands autonomously produce excessive amounts of aldosterone and cortisol, respectively. The conventional adrenal venous sampling (cAVS) method collects blood samples from both adrenal central veins and is useful for identifying the laterality of excess hormone production in a unilateral lesion(s), as documented in PA cases. In cAVS, plasma cortisol concentrations (PCCs) are used to normalize plasma aldosterone concentrations (PACs). A novel "super-selective" adrenal venous sampling (ssAVS) method was developed using a micro-catheter, which collects blood samples from adrenal tributary veins (TVs). PACs in ssAVS samples do not require PCC normalization because samples contain a limited amount of systemic venous blood, if any. The ssAVS method enabled segmental lesion(s) to be detected in both adrenal glands, which may be treated by bilateral adrenalectomy, thereby sparing lesion-free segment(s). Right and left adrenals typically have three TVs each, i.e., the superior, lateral, and inferior TVs in the right adrenal as well as the superior-median, superior-lateral, and lateral TVs in the left adrenal. In the ssAVS method, specific parent catheters and a technique to handle them are required, and have been described herein. Furthermore, ssAVS results from three cases of PA are presented: bilateral aldosterone-producing adenoma (APA) (Case #1), left APA and right possible cortisol-producing adenoma causing SCS (Case #2), and idiopathic hyperaldosteronism in which bilateral adrenal segments produced excessive amounts of aldosterone (Case #3). The ssAVS method is not difficult for expert angiographers, and, thus, is recommended worldwide to treat PA cases for which cAVS does not represent a viable surgical treatment option.
    MeSH term(s) Adrenal Glands/blood supply ; Blood Specimen Collection/methods ; Catheterization, Central Venous ; Catheterization, Peripheral ; Humans ; Vascular Access Devices ; Veins
    Language English
    Publishing date 2017-09-15
    Publishing country United States
    Document type Journal Article ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/55716
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  7. Article ; Online: Expression of aldosterone synthase CYP11B2 was inversely correlated with longevity.

    Hayashi, Taiki / Zhang, Zhen / Al-Eyd, Ghaith / Sasaki, Atsushi / Yasuda, Masanori / Oyama, Masafumi / Gomez-Sanchez, Celso E / Asakura, Hirotaka / Seki, Tsugio / Mukai, Kuniaki / Nishimoto, Koshiro

    The Journal of steroid biochemistry and molecular biology

    2019  Volume 191, Page(s) 105361

    Abstract: Immunohistochemistry of human aldosterone synthase (CYP11B2) has revealed that most of aldosterone is autonomously produced in aldosterone-producing cell clusters (APCCs) beneath the capsule of adult adrenals rather than physiologically in the zona ... ...

    Abstract Immunohistochemistry of human aldosterone synthase (CYP11B2) has revealed that most of aldosterone is autonomously produced in aldosterone-producing cell clusters (APCCs) beneath the capsule of adult adrenals rather than physiologically in the zona glomerulosa (ZG). APCCs have been occasionally found to harbor a somatic mutation of ion channel/pump genes, and number and size of APCCs increase with age until 50 years old. Herein, the objective of the study was to examine APCC development in 106 autopsied adrenals from 85 elderly individuals who died at ages from 50 to 103 years. We obtained the following results: (1) physiological CYP11B2 expression in ZG were attenuated in more elderly persons; (2) number and size of APCCs decreased with age; (3) detachment of APCC from the capsule appeared to occur occasionally over the wide range of the ages; and (4) incidental micro aldosterone-producing adenomas (APAs) and possible APCC-to-APA transitional lesions (pAATLs) were found primarily in samples from persons aged 50-60 years but not in samples from more elderly persons; pAATL was a putative designation based on our previous results indicating that it consisted of subcapsular APCC-like portion and inner APA-like portions. Thus, the formation of the CYP11B2-expressing lesions as well as thickening of the ZG in the adrenals were inversely correlated with age of death in the individuals aged over 50 years. Considering that autopsy samples were used in this study, inactive production of aldosterone regardless of autonomous or physiological manners may have survival advantages in individuals aged over 50 years.
    MeSH term(s) Adrenal Glands/chemistry ; Adrenal Glands/physiology ; Adrenal Glands/ultrastructure ; Aged ; Aged, 80 and over ; Aging ; Aldosterone/metabolism ; Cytochrome P-450 CYP11B2/analysis ; Cytochrome P-450 CYP11B2/metabolism ; Female ; Humans ; Longevity ; Male ; Middle Aged
    Chemical Substances Aldosterone (4964P6T9RB) ; Cytochrome P-450 CYP11B2 (EC 1.14.15.4)
    Language English
    Publishing date 2019-04-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1049188-0
    ISSN 1879-1220 ; 0960-0760
    ISSN (online) 1879-1220
    ISSN 0960-0760
    DOI 10.1016/j.jsbmb.2019.04.010
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    Zs.A 708: Show issues Location:
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  8. Article: Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation.

    Nishimoto, Koshiro / Seki, Tsugio / Hayashi, Yuichiro / Mikami, Shuji / Al-Eyd, Ghaith / Nakagawa, Ken / Morita, Shinya / Kosaka, Takeo / Oya, Mototsugu / Mitani, Fumiko / Suematsu, Makoto / Kabe, Yasuaki / Mukai, Kuniaki

    International journal of endocrinology

    2016  Volume 2016, Page(s) 7834356

    Abstract: ... ...

    Abstract Background
    Language English
    Publishing date 2016-09-18
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 2502951-4
    ISSN 1687-8345 ; 1687-8337
    ISSN (online) 1687-8345
    ISSN 1687-8337
    DOI 10.1155/2016/7834356
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  9. Article ; Online: Lessons from the gene expression pattern of the rat zona glomerulosa.

    Nishimoto, Koshiro / Rainey, William E / Bollag, Wendy B / Seki, Tsugio

    Molecular and cellular endocrinology

    2012  Volume 371, Issue 1-2, Page(s) 107–113

    Abstract: We recently identified hundreds of transcripts with differential expression in rat zona glomerulosa (zG) and zona fasciculata. Although the genes up-regulated in the zG may be playing important roles in aldosterone production, the relationship between ... ...

    Abstract We recently identified hundreds of transcripts with differential expression in rat zona glomerulosa (zG) and zona fasciculata. Although the genes up-regulated in the zG may be playing important roles in aldosterone production, the relationship between most of these genes and aldosterone production has not been uncovered. Because aldosterone, in the presence of a high sodium diet, is now considered a significant cardiovascular risk factor, in this review we performed gene ontology and pathway analyses on the same microarray data to better define the genes that may influence zG function. Overall, we identified a number of genes that may be involved in aldosterone production through transforming growth factor β (TGF-β), WNT, calcium, potassium, and ACTH signaling pathways. The list of genes we present in the current report may become an important tool for researchers working on primary aldosteronism and aldosterone-related cardiovascular diseases.
    MeSH term(s) Adrenocorticotropic Hormone/metabolism ; Aldosterone/biosynthesis ; Animals ; Calcium/metabolism ; Cardiovascular Diseases/genetics ; Cardiovascular Diseases/metabolism ; Gene Expression ; Potassium/metabolism ; Rats ; Signal Transduction ; Transcription, Genetic ; Transforming Growth Factor beta/genetics ; Transforming Growth Factor beta/metabolism ; Wnt Proteins/genetics ; Wnt Proteins/metabolism ; Zona Fasciculata/metabolism ; Zona Glomerulosa/metabolism
    Chemical Substances Transforming Growth Factor beta ; Wnt Proteins ; Aldosterone (4964P6T9RB) ; Adrenocorticotropic Hormone (9002-60-2) ; Potassium (RWP5GA015D) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2012-12-31
    Publishing country Ireland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 187438-x
    ISSN 1872-8057 ; 0303-7207
    ISSN (online) 1872-8057
    ISSN 0303-7207
    DOI 10.1016/j.mce.2012.12.023
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    Zs.A 1127: Show issues Location:
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  10. Article ; Online: Aldosterone and 18-Oxocortisol Coaccumulation in Aldosterone-Producing Lesions.

    Sugiura, Yuki / Takeo, Emi / Shimma, Shuichi / Yokota, Mai / Higashi, Tatsuya / Seki, Tsugio / Mizuno, Yosuke / Oya, Mototsugu / Kosaka, Takeo / Omura, Masao / Nishikawa, Tetsuo / Suematsu, Makoto / Nishimoto, Koshiro

    Hypertension (Dallas, Tex. : 1979)

    2018  Volume 72, Issue 6, Page(s) 1345–1354

    Abstract: Primary aldosteronism is a secondary hypertensive disease caused by autonomous aldosterone production that often caused by an aldosterone-producing adenoma (APA). Immunohistochemistry of aldosterone synthase (CYP11B2) shows the presence of aldosterone- ... ...

    Abstract Primary aldosteronism is a secondary hypertensive disease caused by autonomous aldosterone production that often caused by an aldosterone-producing adenoma (APA). Immunohistochemistry of aldosterone synthase (CYP11B2) shows the presence of aldosterone-producing cell clusters (APCCs) even in non-primary aldosteronism adult adrenal cortex. An APCC-like structure also exists as possible APCC-to-APA transitional lesions (a speculative designation) in primary aldosteronism adrenals. However, whether APCCs produce aldosterone or 18-oxocortisol, a potential serum marker of APA, remains unknown because of lack of technology to visualize adrenocorticosteroids on tissue sections. To address this obstacle, in this study, we used highly sensitive Fourier transform ion cyclotron resonance mass spectrometry to image various adrenocorticosteroids, including 18-oxocortisol, in adrenal tissue sections from 8 primary aldosteronism patients with APCC (cases 1-4), possible APCC-to-APA transitional lesions (case 5), and APA (cases 6-8). Further analyses by tandem mass spectrometry imaging allowed us to differentially visualize aldosterone from cortisone, which share identical mass-to-charge ratio value ( m/z). In conclusion, these advanced imaging techniques revealed that aldosterone and 18-oxocortisol coaccumulated within CYP11B2-expressing lesions. These imaging outcomes along with a growing body of aldosterone research led us to build a progressive development hypothesis of an aldosterone-producing pathology in the adrenal glands.
    MeSH term(s) Adrenal Gland Neoplasms/metabolism ; Adrenal Gland Neoplasms/pathology ; Adrenal Glands/metabolism ; Adrenocortical Adenoma/metabolism ; Adrenocortical Adenoma/pathology ; Adult ; Aldosterone/metabolism ; Female ; Humans ; Hydrocortisone/analogs & derivatives ; Hydrocortisone/metabolism ; Hyperaldosteronism/metabolism ; Hyperaldosteronism/pathology ; Hypertension/metabolism ; Hypertension/pathology ; Male ; Middle Aged
    Chemical Substances 18-oxocortisol (2410-60-8) ; Aldosterone (4964P6T9RB) ; Hydrocortisone (WI4X0X7BPJ)
    Language English
    Publishing date 2018-12-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 423736-5
    ISSN 1524-4563 ; 0194-911X ; 0362-4323
    ISSN (online) 1524-4563
    ISSN 0194-911X ; 0362-4323
    DOI 10.1161/HYPERTENSIONAHA.118.11243
    Database MEDical Literature Analysis and Retrieval System OnLINE

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