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Article ; Online: Highly effective heterogeneous doxycycline stabilized silver nanocatalyst for the degradation of ibuprofen and paracetamol drugs

Yasmeen Junejo / Muhammad Safdar

Arabian Journal of Chemistry, Vol 12, Iss 8, Pp 2823-

2019 Volume 2832

Abstract: In this work, a new doxycycline stabilized silver nanocatalyst (Dox-Ag (0) NPs) was synthesized in aqueous solution (green method) by one-pot simple synthetic method for the ultra-fast catalytic degradation of ibuprofen and paracetamol. The formation of ... ...

Abstract	In this work, a new doxycycline stabilized silver nanocatalyst (Dox-Ag (0) NPs) was synthesized in aqueous solution (green method) by one-pot simple synthetic method for the ultra-fast catalytic degradation of ibuprofen and paracetamol. The formation of the Dox-Ag (0) NPs was monitored using UV–Vis absorption spectroscopy which confirmed the formation of Dox-Ag (0) NPs by exciting the typical surface plasmon absorption maxima at 404 nm. Transmission electron microscopy (TEM) confirmed the spherical morphology and monodispersed Dox-Ag (0) NPs with particle size 6.87 ± 2.2 nm. The newly synthesized Dox-Ag (0) NPs had an excellent catalytic activity as a catalyst for the 100% degradation of ibuprofen and paracetamol, which was carried out in 60 s. The antimicrobial activities of this catalyst were also evaluated against Gram-negative bacteria Mycoplasma hominis, Escherichia coli, Pseudomonas aeruginosa and Gram-positive bacteria Staphylococcus aureus, Micrococcus flavus and Micrococcus luteus by the disk diffusion method. Whereas standard antibiotic showed no zone of inhibition, the Dox-Ag (0) NPs showed good inhibition zone. The antimicrobial results therefore reveal that newly synthesized Dox-Ag (0) NPs had a tremendous catalytic and antimicrobial activity as a catalyst. They were recovered easily from reaction medium and reused with enhanced catalytic potential seven times. The current findings are equally extendable for safeguarding the aquatic environment against the pollution caused by drugs and microbial activity via a facile, highly economical, rapid and efficient reduction/degradation method based on the catalytic potential of Dox-Ag (0) NPs. Keywords: Doxycycline stabilized silver nanocatalyst, Heterogeneous catalysis, Green method, Drugs degradation, Antimicrobial activity
Keywords	Chemistry ; QD1-999
Subject code	540
Language	English
Publishing date	2019-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article: Adrenergic blocker terazosin potentially suppresses acetaminophen induced-acute liver injury in animal models via CYP2E1 gene.

Hashmat, Zoya / Channa, Iffat Saeed / Safdar, Muhammad / Ozaslan, Mehmet / Saeed, Muhammad / Siddique, Faisal / Junejo, Yasmeen

Toxicological research

2022 Volume 38, Issue 3, Page(s) 323–330

Abstract: Drug induced liver injury (DILI) is a global issue and acetaminophen (APAP) is considered as the main cause of this. Due to increasing incidents of DILI, current study attempted to investigate an alternative but better role of terazosin (alpha-adrenergic ...

Abstract	Drug induced liver injury (DILI) is a global issue and acetaminophen (APAP) is considered as the main cause of this. Due to increasing incidents of DILI, current study attempted to investigate an alternative but better role of terazosin (alpha-adrenergic blocker) in APAP-induced acute liver injury in an animal model using New Zealand rabbits. APAP (1 g/kg of body weight) was given to New Zealand rabbits either with or without terazosin (0.5 mg/kg) and serum was collected after 4 h. Serum alanine transaminase (ALT), alkaline phosphatase (ALP) and ferritin level were determined to analyze the liver functioning of treated rabbits. Furthermore, total cholesterol (TC), total lipids (TL), high-density lipoproteins (HDL), low-density lipoprotein (LDL) and triglycerides (TG) levels were estimated to find any change in lipid profile of the treated animals. Moreover, the urea and creatinine levels assayed the actual renal functionality. To identify any modification in gene expression, qPCR of cytochrome P2E1 (CYP2E1) was performed. Terazosin in combination with APAP enhanced liver functioning by reducing the levels of liver injury markers viz. ALP and ALT, while lipid profile was also lowered by down regulation of TC, TL, LDL and TG with enhanced HDL levels. It caused significant down regulation of expression level of CYP2E1. It is concluded that terazosin has better effects induced on the recovery of normal liver functioning, by improving the liver profile, lipid profile and renal functioning both at tissue and molecular levels.
Language	English
Publishing date	2022-01-11
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2727978-9
ISSN	2234-2753 ; 1976-8257
ISSN (online)	2234-2753
ISSN	1976-8257
DOI	10.1007/s43188-021-00116-y
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Article: Development and validation of fast duplex real-time PCR assays based on SYBER Green florescence for detection of bovine and poultry origins in feedstuffs.

Safdar, Muhammad / Junejo, Yasmeen

Food chemistry

2015 Volume 173, Page(s) 660–664

Abstract: SYBR duplex real-time polymerase chain reaction (SDRT-PCR) with melting curve analysis was developed that can unite the reward of multiplex PCR with real-time PCR to recognize animal genes rapidly in feedstuffs. The method merges the use of bovine (Bos ... ...

Abstract	SYBR duplex real-time polymerase chain reaction (SDRT-PCR) with melting curve analysis was developed that can unite the reward of multiplex PCR with real-time PCR to recognize animal genes rapidly in feedstuffs. The method merges the use of bovine (Bos taurus) and poultry (Gallus gallus) specific primers that amplify small fragments (amplicons <200 base pairs) of the mitochondrial gene. Appropriate mixtures of bovine and poultry heat treated meat DNAs were used to develop the assay. Gene products of bovine and poultry were produced two distinct melting peaks simultaneously at 79.5 °C and 87.5 °C, respectively. Multiplex analysis of the reference feed samples showed that the detection limit of the assay was 0.001% for bovine and poultry species. Based upon the assay results it has been concluded that SDRT-PCR assay might be an efficient tool for the verification of species origin in feedstuffs.
MeSH term(s)	Animal Feed/analysis ; Animals ; Cattle/genetics ; DNA, Mitochondrial/analysis ; Meat/analysis ; Organic Chemicals ; Poultry/genetics ; Real-Time Polymerase Chain Reaction/methods
Chemical Substances	DNA, Mitochondrial ; Organic Chemicals ; SYBR Green I (163795-75-3)
Language	English
Publishing date	2015-04-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2014.10.088
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Article ; Online: The development of a hexaplex-conventional PCR for identification of six animal and plant species in foodstuffs.

Safdar, Muhammad / Junejo, Yasmeen

Food chemistry

2015 Volume 192, Page(s) 745–749

Abstract: A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), sheep (Ovis aries), poultry (Meleagris ...

Abstract	A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), sheep (Ovis aries), poultry (Meleagris meleagris), pork (Sus scrofa), and cow (Bos taurus) specific primers that amplify fragments (horse; 85 bp, soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, pork; 212 bp and cow; 271 bp) of the mitochondrial cyt b, lectin, 12S rRNA, 12S rRNA, ATPase subunit 6 genes and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 141 bp. Multiplex analysis of the reference food samples showed that detection limit of the hexaplex assay was 0.01% for each species. Taken together, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and cost-effective detection method for horse, soybean, sheep, poultry, pork and cow species in foodstuffs.
MeSH term(s)	Adenosine Triphosphatases/genetics ; Animals ; Cattle ; DNA Primers ; DNA, Mitochondrial/genetics ; Food Analysis/methods ; Food Handling ; Horses ; Meat/analysis ; Polymerase Chain Reaction/methods ; Poultry ; RNA, Ribosomal/genetics ; RNA, Ribosomal, 18S/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; Sheep ; Glycine max/chemistry ; Swine
Chemical Substances	DNA Primers ; DNA, Mitochondrial ; RNA, Ribosomal ; RNA, Ribosomal, 18S ; RNA, ribosomal, 12S ; Adenosine Triphosphatases (EC 3.6.1.-)
Language	English
Publishing date	2015-07-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2015.07.082
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Article: The development of a hexaplex-conventional PCR for identification of six animal and plant species in foodstuffs

Safdar, Muhammad / Yasmeen Junejo

Food chemistry. 2016 Feb. 01, v. 192

2016

Abstract	A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), sheep (Ovis aries), poultry (Meleagris meleagris), pork (Sus scrofa), and cow (Bos taurus) specific primers that amplify fragments (horse; 85bp, soybean; 100bp, sheep; 119bp, poultry; 183bp, pork; 212bp and cow; 271bp) of the mitochondrial cyt b, lectin, 12S rRNA, 12S rRNA, ATPase subunit 6 genes and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 141bp. Multiplex analysis of the reference food samples showed that detection limit of the hexaplex assay was 0.01% for each species. Taken together, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and cost-effective detection method for horse, soybean, sheep, poultry, pork and cow species in foodstuffs.
Keywords	adenosinetriphosphatase ; cost effectiveness ; cows ; detection limit ; genes ; Glycine max ; horses ; lectins ; Meleagris ; mitochondria ; polymerase chain reaction ; pork ; poultry ; ribosomal RNA ; sheep ; soybeans ; Sus scrofa
Language	English
Dates of publication	2016-0201
Size	p. 745-749.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2015.07.082
Database	NAL-Catalogue (AGRICOLA)

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Article: Novel SARS-CoV-2/COVID-19: Origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis.

Junejo, Yasmeen / Ozaslan, Mehmet / Safdar, Muhamad / Khailany, Rozhgar A / Rehman, SaifUr / Yousaf, Wasim / Khan, Musarrat Abbas

Gene reports

2020 Volume 20, Page(s) 100752

Abstract: In this review, we focused on the origins of the novel coronavirus (SARS-CoV-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing ... ...

Abstract	In this review, we focused on the origins of the novel coronavirus (SARS-CoV-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing broadly effective preventions and vaccines to cope COVID-19. Towards the end of 2019, SARS-CoV-2 has emerged in association with the SARS, later was named COVID-19 caused an environment of chaos worldwide and infected a massive number of lives. Since these epidemics or pandemics had spread to 210 countries and territories around the world and 2 international conveyances with 6,467,229 confirmed cases, including, 382,766 deaths, as of June 03, 2020 (https://www.worldometers.info/coronavirus/), hence the World Health Organization declared it as a global Public Health Emergency. There are no clinically approved vaccines or antiviral drugs available for either of new or old corona infections; thus, the development of effective therapeutic and preventive strategies that can be readily available to cope with these strains.
Keywords	covid19
Language	English
Publishing date	2020-06-15
Publishing country	United States
Document type	Journal Article
ISSN	2452-0144
ISSN	2452-0144
DOI	10.1016/j.genrep.2020.100752
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Article: A multiplex-conventional PCR assay for bovine, ovine, caprine and fish species identification in feedstuffs: Highly sensitive and specific

Safdar, Muhammad / Yasmeen Junejo

Food control. 2015 Apr., v. 50

2015

Abstract: A multiplex PCR assay was developed for rapid and reliable identification of bovine, ovine, caprine and fish species in feedstuffs simultaneously. The method merges the use of bovine, ovine, caprine and fish primers that amplify fragments (ovine; 119Â bp, ...

Abstract	A multiplex PCR assay was developed for rapid and reliable identification of bovine, ovine, caprine and fish species in feedstuffs simultaneously. The method merges the use of bovine, ovine, caprine and fish primers that amplify fragments (ovine; 119Â bp, caprine; 142Â bp, fish; 224Â bp and bovine; 271Â bp) of the mitochondrial t.glu gene forward and cyt b reverse, 12S rRNA, 12S rRNA, and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 99Â bp from eukaryotic DNA. To evaluate the effect of heat treatment, a severe sterilization condition (133Â Â°C at 300Â kPa for 20Â min) was applied. Multiplex analysis of the reference feedstuff samples showed that the detection limit of the assay was 0.01% for each species. Taken together, all data indicated that this multiplex PCR assay was a simple, rapid, sensitive, specific, and cost-effective detection method for bovine, ovine, caprine and fish species in feedstuffs.
Keywords	adenosinetriphosphatase ; cattle ; cost effectiveness ; detection limit ; DNA ; feeds ; fish ; genes ; goats ; heat treatment ; mitochondria ; polymerase chain reaction ; ribosomal RNA ; sheep ; species identification
Language	English
Dates of publication	2015-04
Size	p. 190-194.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1027805-9
ISSN	0956-7135
ISSN	0956-7135
DOI	10.1016/j.foodcont.2014.08.048
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: SNPs at 3'UTR of APOL1 and miR-6741-3p target sites associated with kidney diseases more susceptible to SARS-COV-2 infection: in silco and in vitro studies.

Safdar, Muhammad / Khan, Muhammad Sajjad / Karim, Abdulkarim Yasin / Omar, Shwan Ali / Smail, Shukur Wasman / Saeed, Muhammad / Zaheer, Sana / Ali, Mazhar / Ahmad, Bilal / Tasleem, Muhammad / Junejo, Yasmeen

Mammalian genome : official journal of the International Mammalian Genome Society

2021 Volume 32, Issue 5, Page(s) 389–400

Abstract: Acute Kidney Injury (AKI) is a common manifestation of COVID-19 and several cases have been reported in the setting of the high-risk APOL1 genotype (common genetic variants). This increases the likelihood that African American people with the high-risk ... ...

Abstract	Acute Kidney Injury (AKI) is a common manifestation of COVID-19 and several cases have been reported in the setting of the high-risk APOL1 genotype (common genetic variants). This increases the likelihood that African American people with the high-risk genotype APOL1 are at increased risk for kidney disease in the COVID-19 environment. Single-nucleotide polymorphisms (SNPs) are found in various microRNAs (miRNAs) and target genes change the miRNA activity that leads to different diseases. Evidence has shown that SNPs increase/decrease the effectiveness of the interaction between miRNAs and disease-related target genes. The aim of this study is not only to identify miRSNPs on the APOL1 gene and SNPs in miRNA genes targeting 3'UTR but also to evaluate the effect of these gene variations in kidney patients and their association with SARS-COV-2 infection. In 3'UTR of the APOL1 gene, we detected 96 miRNA binding sites and 35 different SNPs with 10 different online software in the binding sites of the miRNA (in silico). Also we studied gene expression of patients and control samples by using qRT-PCR (in vitro). In silico study, the binding site of miR-6741-3p on APOL1 has two SNPs (rs1288875001, G > C; rs1452517383, A > C) on APOL1 3'UTR, and its genomic sequence is the same nucleotide as rs1288875001. Similarly, two other SNPs (rs1142591, T > A; rs376326225, G > A) were identified in the binding sites of miR-6741-3p at the first position. Here, the miRSNP (rs1288875001) in APOL1 3'UTR and SNP (rs376326225) in the miR-6741-3p genomic sequence are cross-matched in the same binding region. In vitro study, the relative expression levels were calculated by the 2
MeSH term(s)	3' Untranslated Regions/genetics ; Apolipoprotein L1/genetics ; Binding Sites/genetics ; COVID-19/genetics ; Case-Control Studies ; Genotype ; Humans ; Kidney/pathology ; Kidney Diseases/genetics ; MicroRNAs/genetics ; Polymorphism, Single Nucleotide/genetics ; SARS-CoV-2/pathogenicity
Chemical Substances	3' Untranslated Regions ; APOL1 protein, human ; Apolipoprotein L1 ; MIRN6744 microRNA, human ; MicroRNAs
Language	English
Publishing date	2021-06-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	1058547-3
ISSN	1432-1777 ; 0938-8990
ISSN (online)	1432-1777
ISSN	0938-8990
DOI	10.1007/s00335-021-09880-6
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Article ; Online: Novel SARS-CoV-2/COVID-19

Junejo, Yasmeen / Ozaslan, Mehmet / Safdar, Muhamad / Khailany, Rozhgar A. / Rehman, SaifUr / Yousaf, Wasim / Khan, Musarrat Abbas

Gene Reports

Origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis

2020 Volume 20, Page(s) 100752

Keywords	covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ISSN	2452-0144
DOI	10.1016/j.genrep.2020.100752
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: SNPs at 3′UTR of APOL1 and miR-6741-3p target sites associated with kidney diseases more susceptible to SARS-COV-2 infection: in silco and in vitro studies

Mammalian genome. 2021 Oct., v. 32, no. 5

2021

Abstract	Acute Kidney Injury (AKI) is a common manifestation of COVID-19 and several cases have been reported in the setting of the high-risk APOL1 genotype (common genetic variants). This increases the likelihood that African American people with the high-risk genotype APOL1 are at increased risk for kidney disease in the COVID-19 environment. Single-nucleotide polymorphisms (SNPs) are found in various microRNAs (miRNAs) and target genes change the miRNA activity that leads to different diseases. Evidence has shown that SNPs increase/decrease the effectiveness of the interaction between miRNAs and disease-related target genes. The aim of this study is not only to identify miRSNPs on the APOL1 gene and SNPs in miRNA genes targeting 3′UTR but also to evaluate the effect of these gene variations in kidney patients and their association with SARS-COV-2 infection. In 3′UTR of the APOL1 gene, we detected 96 miRNA binding sites and 35 different SNPs with 10 different online software in the binding sites of the miRNA (in silico). Also we studied gene expression of patients and control samples by using qRT-PCR (in vitro). In silico study, the binding site of miR-6741-3p on APOL1 has two SNPs (rs1288875001, G > C; rs1452517383, A > C) on APOL1 3′UTR, and its genomic sequence is the same nucleotide as rs1288875001. Similarly, two other SNPs (rs1142591, T > A; rs376326225, G > A) were identified in the binding sites of miR-6741-3p at the first position. Here, the miRSNP (rs1288875001) in APOL1 3′UTR and SNP (rs376326225) in the miR-6741-3p genomic sequence are cross-matched in the same binding region. In vitro study, the relative expression levels were calculated by the 2⁻ΔΔCᵗ method & Mann–Whitney U test. The expression of APOL1 gene was different in chronic kidney patients along with COVID-19. By these results, APOL1 expression was found lower in patients than healthy (p < 0.05) in kidney patients along with COVID-19. In addition, miR-6741-3p targets many APOL1-related genes (TLR7, SLC6A19, IL-6,10,18, chemokine (C–C motif) ligand 5, SWT1, NFYB, BRF1, HES2, NFYB, MED12L, MAFG, GTF2H5, TRAF3, angiotensin II receptor-associated protein, PRSS23) by evaluating online software in the binding sites of the miR-6741-3p. miR-6741-3p has not previously shown any association with kidney diseases and SARS-COV-2 infection. It assures that APOL1 can have a significant consequence in kidney-associated diseases by different pathways. Henceforth, this study represents and demonstrates an effective association between miR-6741-3p and kidney diseases, i.e., collapsing glomerulopathy, chronic kidney disease (CKD), acute kidney injury (AKI), and tubulointerstitial lesions susceptibility to SARS-COV-2 infection via in silico and in vitro exploration and recommended to have better insight.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; acute kidney injury ; computer simulation ; computer software ; gene expression ; genes ; genotype ; glomerulopathy ; kidneys ; ligands ; mammals ; microRNA ; risk
Language	English
Dates of publication	2021-10
Size	p. 389-400.
Publishing place	Springer US
Document type	Article
ZDB-ID	1058547-3
ISSN	1432-1777 ; 0938-8990
ISSN (online)	1432-1777
ISSN	0938-8990
DOI	10.1007/s00335-021-09880-6
Database	NAL-Catalogue (AGRICOLA)

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