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Article ; Online: Structural and numerical Y chromosomal variations in elderly men identified through multiplex ligation-dependent probe amplification.

Ogiwara, Yasuko / Miyado, Mami / Suzuki, Erina / Niida, Sumpei / Ozaki, Kouichi / Fukami, Maki

2021 Volume 66, Issue 12, Page(s) 1181–1184

Abstract: Human Y chromosomes frequently acquire structural and numerical alterations. Known alterations ... loss of the Y chromosome (mLOY). Here, we explored Y chromosomal variations in 160 Japanese men aged 75 ...

Abstract	Human Y chromosomes frequently acquire structural and numerical alterations. Known alterations include germline copy-number variations (CNVs) in the azoospermia factor (AZF) region and somatic mosaic loss of the Y chromosome (mLOY). Here, we explored Y chromosomal variations in 160 Japanese men aged 75-90 years. Multiplex ligation-dependent probe amplification (MLPA) identified ten types of AZF-linked CNVs in 77 men and mLOY of various degrees in 37. Seventeen men carried both a CNV and mLOY. MLOY levels estimated by MLPA were closely correlated with those determined by droplet digital PCR. No association was found between AZF-linked CNVs and the frequency or levels of mLOY. These results emphasize the high frequency and large inter-individual variability of AZF-linked CNVs and mLOY, and demonstrate the usefulness of MLPA in the detection of these variations. More importantly, this study provides the first evidence that AZF-linked CNVs do not increase the risk of aging-related mLOY.
MeSH term(s)	Aged ; Chromosome Aberrations ; Chromosomes, Human, Y/genetics ; DNA Copy Number Variations ; Genetic Association Studies ; Genetic Variation ; Humans ; Male ; Phenotype
Language	English
Publishing date	2021-06-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1425192-9
ISSN	1435-232X ; 1434-5161
ISSN (online)	1435-232X
ISSN	1434-5161
DOI	10.1038/s10038-021-00943-9
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Zs.A 201: Show issues

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Article ; Online: Neuropeptide Y neuronal network dysfunction in the frontal lobe of a genetic mouse model of schizophrenia.

Morosawa, Shunsuke / Iritani, Shuji / Fujishiro, Hiroshige / Sekiguchi, Hirotaka / Torii, Youta / Habuchi, Chikako / Kuroda, Keisuke / Kaibuchi, Kozo / Ozaki, Norio

Neuropeptides

2017 Volume 62, Page(s) 27–35

Abstract: Neuropeptide Y (NPY) has been found to play a critical role in various mental functions ...

Abstract	Neuropeptide Y (NPY) has been found to play a critical role in various mental functions as a neurotransmitter and is involved in the development of schizophrenia, a particularly intractable psychiatric disease whose precise etiology remains unknown. Recent molecular biological investigations have identified several candidate genes which may be associated with this disease, including disrupted-in-schizophrenia 1 (DISC1). The role of DISC1 would involve neurogenesis and neuronal migration. However, the functional consequences of this gene defect have not yet been fully clarified in neuronal systems. In the present study, to clarify the neuropathological changes associated with the function of DISC1, we explored how DISC1 dysfunction can induce abnormalities in the NPY neuronal network in the central nervous system. We performed immunohistochemical analyses (including the observation of the distribution and density) of prefrontal cortex specimens from DISC1-knockout (KO) mice, which are considered to be a novel animal model of schizophrenia. We then evaluated the number and size of NPY-immunoreactive (NPY-IR) neurons and the length of NPY-IR fibers. The number of NPY-IR neurons and the length of the fibers were decreased in the prefrontal cortex of DISC1-KO mice. The decrease was particularly prominent in the superficial regions, and the distribution of NPY-IR neurons differed between wild-type and DISC1-KO mice. However, the size of the neurons in the cortices of the DISC1-KO and wild-type mice did not differ markedly. Our findings suggest that dysfunction of DISC1 may lead to the alteration of NPY neurons and neurotransmission issues in NPY-containing neuron systems, which seem to play important roles in both the mental function and neuronal development. DISC1 dysfunction may be involved in the pathogenesis of schizophrenia through the impairment of the NPY neuronal network.
Language	English
Publishing date	2017-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	9048-7
ISSN	1532-2785 ; 0143-4179
ISSN (online)	1532-2785
ISSN	0143-4179
DOI	10.1016/j.npep.2016.12.010
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Zs.A 1592: Show issues

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Article: [A Case of Intestinal Diverticular Perforation in Roux-en-Y Reconstruction after Laparoscopic Distal Gastrectomy].

Tanaka, Hideyuki / Doi, Koichi / Ogawa, Katsuhiro / Yagi, Taisuke / Umesaki, Naoki / Ozaki, Nobuyuki / Hayashi, Hiromitsu / Ikuta, Yoshiaki / Ogata, Kenichi / Takamori, Hiroshi

Gan to kagaku ryoho. Cancer & chemotherapy

2016 Volume 43, Issue 12, Page(s) 1902–1904

Abstract: An 81-year-old woman who had undergone laparoscopic distal gastrectomy complained of abdominal pain 21 days after the operation.Blood tests showed a strong inflammatory reaction.Abdominal CT revealed a perforation in the small intestinal diverticula ... ...

Abstract	An 81-year-old woman who had undergone laparoscopic distal gastrectomy complained of abdominal pain 21 days after the operation.Blood tests showed a strong inflammatory reaction.Abdominal CT revealed a perforation in the small intestinal diverticula.Partial jejunectomy including the diverticulum was performed.The diverticular perforation was attributed to the presence of undigested food in the diverticulum.The patient had an uneventful postoperative course, and she was discharged on postoperative day 32.
MeSH term(s)	Aged, 80 and over ; Anastomosis, Roux-en-Y ; Female ; Gastrectomy ; Humans ; Intestinal Perforation/etiology ; Intestinal Perforation/surgery ; Jejunal Diseases/etiology ; Jejunal Diseases/surgery ; Laparoscopy ; Stomach Neoplasms/surgery
Language	Japanese
Publishing date	2016-11
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	604842-0
ISSN	0385-0684
ISSN	0385-0684
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Zs.B 2573: Show issues

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Article: The gene pair PRR11 and SKA2 shares a NF-Y-regulated bidirectional promoter and contributes to lung cancer development.

Wang, Yitao / Zhang, Ying / Zhang, Chundong / Weng, Huali / Li, Yi / Cai, Wei / Xie, Mengyu / Long, Yinjiang / Ai, Qing / Liu, Zhu / Du, Gang / Wang, Sen / Niu, Yulong / Song, Fangzhou / Ozaki, Toshinori / Bu, Youquan

Biochimica et biophysica acta

2015 Volume 1849, Issue 9, Page(s) 1133–1144

Abstract: ... assays demonstrated that NF-Y binds to and directly transactivates the PRR11-SKA2 bidirectional promoter ... SiRNA-mediated NF-Y depletion significantly downregulated PRR11 and SKA2 expression. Expression ... correlated with each other and with NF-Y expression. Remarkably, high expression of both PRR11 and SKA2 was ...

Abstract	Head-to-head gene pairs represent a unique feature of gene organization in eukaryotes, accounting for >10% of genes in the human genome. Identification and functional analysis of such gene pairs is only in its infancy. Recently, we identified PRR11 as a novel cancer-related gene that is implicated in cell cycle and lung cancer. Here we demonstrate that PRR11 is oriented in a head-to-head configuration with its neighboring gene, SKA2. 5'-RACE assay revealed that the intergenic spacer region between the two genes is <500 bp. Serial luciferase reporter assays demonstrated that a minimal 80-bp intergenic region functions as a core bidirectional promoter to drive basal transcription in both the PRR11 and SKA2 orientations. EMSA and ChIP assays demonstrated that NF-Y binds to and directly transactivates the PRR11-SKA2 bidirectional promoter. SiRNA-mediated NF-Y depletion significantly downregulated PRR11 and SKA2 expression. Expression of both PRR11 and SKA2 was significantly upregulated in lung cancer. Expression of the two genes was highly correlated with each other and with NF-Y expression. Remarkably, high expression of both PRR11 and SKA2 was associated with poorer prognosis in lung cancer patients compared with high expression of one gene or low expression of both genes. Knockdown of PRR11 and/or SKA2 remarkably reduced cell proliferation, migration, and invasion in lung cancer cells. Thus, the PRR11-SKA2 bidirectional transcription unit, which is a novel direct target of NF-Y, is essential for the accelerated proliferation and motility of lung cancer cells and may represent a potential target in the diagnosis and/or treatment of human lung cancer.
MeSH term(s)	Animals ; Base Sequence ; CCAAT-Binding Factor/physiology ; Cell Line, Tumor ; Chromatin/genetics ; Chromosomal Proteins, Non-Histone/genetics ; DNA/genetics ; Gene Expression Regulation ; Humans ; Lung Neoplasms/genetics ; Molecular Sequence Data ; Promoter Regions, Genetic ; Proteins/genetics ; Sequence Homology, Nucleic Acid ; Transcription, Genetic
Chemical Substances	CCAAT-Binding Factor ; Chromatin ; Chromosomal Proteins, Non-Histone ; PRR11 protein, human ; Proteins ; SKA2 protein, human ; DNA (9007-49-2)
Language	English
Publishing date	2015-07-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbagrm.2015.07.002
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Uc I Zs.247: Show issues

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Article ; Online: Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats.

Ozawa, Yoshiharu / Arima, Hiroshi / Watanabe, Minemori / Shimizu, Hiroshi / Ito, Yoshihiro / Banno, Ryoichi / Sugimura, Yoshihisa / Ozaki, Nobuaki / Nagasaki, Hiroshi / Oiso, Yutaka

Peptides

2011 Volume 32, Issue 4, Page(s) 763–769

Abstract: ... as activities of orexigenic neuropeptide Y (NPY) neurons in repeated glucoprivation in rats. Either 2-deoxy-d ...

Abstract	It is well known that glucoprivation induces the release of counterregulatory hormones such as glucagon, and that the response is attenuated when the stimuli are repeated. Glucoprivation also activates orexigenic neurons and induces hyperphagic responses, although it remains unclear whether these responses are attenuated in repeated glucoprivation. In this study, we examined time course changes in feeding as well as activities of orexigenic neuropeptide Y (NPY) neurons in repeated glucoprivation in rats. Either 2-deoxy-d-glucose (2DG), which blocks glucose utilization, or isotonic saline (control) was injected subcutaneously to rats for 14 days, and food consumption for 1 and 2h after injection was monitored throughout the experiment. While 2DG injection induced robust feeding responses during the first 1h after injection, the response was gradually attenuated and the food consumption was significantly less on days 12-14 compared to that on day 1. On the other hand, food consumption during 2h after 2DG injection was not changed significantly for 14 days. The transcriptional activities of NPY neurons in the arcuate nucleus and C1/A1 region of the hindbrain, measured by intronic in situ hybridization, were significantly enhanced after repeated 2DG injection for 14 days, while the feeding responses to intracerebroventricular injection of NPY were significantly less in the 2DG-repeated group compared to the saline-repeated group. It is thus demonstrated that repeated glucoprivation delayed hyperphagic responses while activating NPY neurons in rats. Our data also suggest that decreased feeding responses to NPY might be at least partially responsible for the delayed response.
MeSH term(s)	Animals ; Blood Glucose/analysis ; Deoxyglucose/administration & dosage ; Hyperphagia/prevention & control ; Insulin/administration & dosage ; Male ; Neurons/drug effects ; Neurons/metabolism ; Neuropeptide Y/metabolism ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Blood Glucose ; Insulin ; Neuropeptide Y ; Deoxyglucose (9G2MP84A8W)
Language	English
Publishing date	2011-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	769028-9
ISSN	1873-5169 ; 0196-9781
ISSN (online)	1873-5169
ISSN	0196-9781
DOI	10.1016/j.peptides.2010.12.009
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Zs.A 1649: Show issues

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Article ; Online: Letter to the editor concerning "Li P., lv Y., zhou F., et al. medial wall fragment involving large posterior cortex in Pertrochanteric femur fractures: A notable preoperative risk factor for implant failure. injury. 2020".

Yamamoto, Norio / Noda, Tomoyuki / Ozaki, Toshifumi

Injury

2020 Volume 51, Issue 4, Page(s) 1146

MeSH term(s)	Femoral Fractures ; Femur ; Humans ; Risk Factors
Language	English
Publishing date	2020-02-20
Publishing country	Netherlands
Document type	Letter ; Comment
ZDB-ID	218778-4
ISSN	1879-0267 ; 0020-1383
ISSN (online)	1879-0267
ISSN	0020-1383
DOI	10.1016/j.injury.2020.02.078
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Zs.A 778: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 10: Show issues

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Article ; Online: Lesion of area postrema attenuated hyperphagic responses to glucoprivation, but not transcriptional activation of the neuropeptide Y gene in rats.

Ozawa, Yoshiharu / Arima, Hiroshi / Banno, Ryoichi / Ito, Yoshihiro / Goto, Motomitsu / Morishita, Yoshiaki / Sugimura, Yoshihisa / Ozaki, Nobuaki / Nagasaki, Hiroshi / Oiso, Yutaka

Neuroreport

2012 Volume 23, Issue 11, Page(s) 673–675

Abstract: ... As the orexigenic neuropeptide Y (NPY) neurons have been implicated in the regulation of food intake, we examined ...

Abstract	The area postrema (AP) is a circumventricular organ that lacks a blood-brain barrier. Previous studies have shown that the lesion of AP (APX) attenuated hyperphagic responses to glucoprivation. As the orexigenic neuropeptide Y (NPY) neurons have been implicated in the regulation of food intake, we examined whether the activation of NPY neurons by glucoprivation is mediated through the AP as well. In agreement with previous studies, hyperphagic responses to an injection of 2-deoxy-D-glucose that blocks glucose utilization were significantly attenuated in the APX group compared with the sham-operated (Sham) group. However, the expression levels of NPY heteronuclear RNA, a sensitive indicator for the gene transcription, were significantly increased in the arcuate nucleus by a 2-deoxy-D-glucose injection in both the APX and the Sham groups, and there were no significant differences in the values between groups. These data suggest that the hyperphagic response to glucoprivation, but not the activation of NPY gene transcription in the arcuate nucleus, was mediated through the AP in the hindbrain.
MeSH term(s)	Animals ; Area Postrema/metabolism ; Area Postrema/physiology ; Deoxyglucose/pharmacology ; Eating/physiology ; Glucose/metabolism ; Hyperphagia ; Male ; Neuropeptide Y/metabolism ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley ; Transcriptional Activation
Chemical Substances	Neuropeptide Y ; RNA, Messenger ; Deoxyglucose (9G2MP84A8W) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2012-08-01
Publishing country	England
Document type	Journal Article
ZDB-ID	1049746-8
ISSN	1473-558X ; 0959-4965
ISSN (online)	1473-558X
ISSN	0959-4965
DOI	10.1097/WNR.0b013e3283556676
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Zs.A 3118: Show issues

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Article ; Online: Proximal Roux-en-y Gastrojejunal Anastomosis with Pyloric Ring Resection Improves Gastric Emptying After Pancreaticoduodenectomy.

Barakat, Omar / Cagigas, Martha N / Bozorgui, Shima / Ozaki, Claire F / Wood, R Patrick

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

2016 Volume 20, Issue 5, Page(s) 914–923

Abstract: ... en-y gastrojejunal anastomosis (group I, n = 90) and patients who underwent standard ...

Abstract	Background: Delayed gastric emptying (DGE) is a common complication of pancreaticoduodenectomy. We determined the efficiency of a new reconstruction technique, designed to preserve motilin-secreting cells and maximize the utility of their receptors, in reducing the incidence of DGE after pancreaticoduodenectomy. Methods: From April 2005 to September 2014, 217 consecutive patients underwent pancreaticoduodenectomy at our institution. Nine patients who underwent total pancreatectomy were excluded. We compared outcomes between patients who underwent pancreaticoduodenectomy with resection of the pyloric ring followed by proximal Roux-en-y gastrojejunal anastomosis (group I, n = 90) and patients who underwent standard pancreaticoduodenectomy with the orthotopic reconstruction technique (group II, n = 118). Results: Overall and clinically relevant rates of DGE were significantly lower in group I than in group II (10 and 2.2 % vs. 57 and 24 %, respectively; p < 0.05). Length of hospital stay as a result of DGE was shorter in group I than in group II. In univariate analysis, older age, comorbidities, ASA grade 4, operative time, preoperative diabetes, standard reconstruction technique, and postoperative complications were significant risk factors for DGE. In multivariate analysis, older age, standard technique, and postoperative complications were independent risk factors for DGE. Conclusion: Our new reconstruction technique reduces the occurrence of DGE after pancreaticoduodenectomy.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Anastomosis, Roux-en-Y/adverse effects ; Female ; Gastric Emptying ; Gastroparesis/etiology ; Gastroparesis/physiopathology ; Gastroparesis/surgery ; Humans ; Jejunum/surgery ; Male ; Middle Aged ; Pancreaticoduodenectomy/adverse effects ; Pilot Projects ; Prospective Studies ; Pyloric Antrum/surgery ; Pylorus/physiopathology ; Pylorus/surgery ; Stomach/physiopathology ; Stomach/surgery ; Treatment Outcome
Language	English
Publishing date	2016-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2012365-6
ISSN	1873-4626 ; 1934-3213 ; 1091-255X
ISSN (online)	1873-4626 ; 1934-3213
ISSN	1091-255X
DOI	10.1007/s11605-016-3091-5
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Zs.A 5207: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Impact of IVUS Guidance on Single-Session vs Staged Multivessel PCI.

Ozaki, Yukio

JACC. Asia

2023 Volume 3, Issue 4, Page(s) 662–663

Language	English
Publishing date	2023-06-13
Publishing country	United States
Document type	Editorial
ISSN	2772-3747
ISSN (online)	2772-3747
DOI	10.1016/j.jacasi.2023.04.009
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Book ; Online ; E-Book: Vibrational spectroscopy in protein research

Ozaki, Yukihiro / Baranska, Malgorzata / Lednev, Igor K. / Wood, Bayden R.

from purified proteins to aggregates and assemblies

2020

Author's details	edited by Yukihiro Ozaki, Malgorzata Baranska, Igor K. Lednev, Bayden R. Wood,
Keywords	Electronic books
Language	English
Size	1 Online-Ressource (xxii, 585 Seiten), Illustrationen, Diagramme
Publisher	Elsevier Academic Press
Publishing place	London
Publishing country	Great Britain
Document type	Book ; Online ; E-Book
Remark	Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
HBZ-ID	HT020486028
ISBN	978-0-12-818611-4 ; 9780128186107 ; 0-12-818611-9 ; 0128186100
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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