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  1. Article ; Online: Efficient and repeatable in vitro fertilization in rabbits.

    Hamze, J G / Peris-Frau, P / Galiano-Cogolludo, B / Tomás-Almenar, C / Santiago-Moreno, J / Bermejo-Álvarez, P

    Theriogenology

    2024  Volume 217, Page(s) 64–71

    Abstract: Rabbits constitute an interesting model to understand gamete interaction and test novel Artificial Reproductive Techniques, but in vitro fertilization (IVF) is particularly problematic in this species. We have conducted a series of experiments to develop ...

    Abstract Rabbits constitute an interesting model to understand gamete interaction and test novel Artificial Reproductive Techniques, but in vitro fertilization (IVF) is particularly problematic in this species. We have conducted a series of experiments to develop a consistent IVF technique. Initially, we checked viability, acrosome integrity, capacitation and motility in ejaculated sperm purified by a density gradient and incubated at different times in three different media: Tyrode's Albumin Lactate Pyruvate (TALP), human tubal fluid (HTF), and Brackett and Oliphant (BO). Total and progressive motility at 10-24 h and linearity from 3 h onwards was significantly higher in BO medium compared to TALP and HTF. Subsequently, cumulus-oocyte complexes (COCs) collected 10 h after induction of ovulation were incubated with sperm in TALP, HTF or BO for 18 h with or without performing sperm pre-incubation for 6 h. Pronuclear formation rate at 18 h was significantly higher in BO compared to other media (∼84 % vs. 17-22 %) and was not improved by pre-incubation. As COCs recovery rate was low at 10 h after induction of ovulation, COCs were collected at 12 h and co-incubated with sperm in BO. Pronuclear formation rate was similar than those obtained in COCs collected at 10 h (∼85 %), and when embryos were allowed to develop in vitro, the protocol yielded high cleavage and blastocyst rates (91 and 59 %, respectively). In conclusion, ejaculated rabbit sperm purified in a density gradient fertilize efficiently COCs collected at 12 h in BO medium.
    MeSH term(s) Female ; Rabbits ; Male ; Humans ; Animals ; Semen ; Fertilization in Vitro/veterinary ; Fertilization in Vitro/methods ; Acrosome ; Spermatozoa ; Oocytes ; Albumins ; Sperm Capacitation
    Chemical Substances Albumins
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/j.theriogenology.2024.01.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3929: Show issues Location:
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  2. Article ; Online: TtCel7A: A Native Thermophilic Bifunctional Cellulose/Xylanase Exogluclanase from the Thermophilic Biomass-Degrading Fungus

    López-López, Azucena / Santiago-Hernández, Alejandro / Cayetano-Cruz, Maribel / García-Huante, Yolanda / Campos, Jorge E / Bustos-Jaimes, Ismael / Marsch-Moreno, Rodolfo / Cano-Ramírez, Claudia / Benitez-Cardoza, Claudia G / Hidalgo-Lara, María Eugenia

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 2

    Abstract: The biomass-degrading thermophilic ascomycete ... ...

    Abstract The biomass-degrading thermophilic ascomycete fungus
    Language English
    Publishing date 2023-01-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9020152
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  3. Article ; Conference proceedings: ESD for the treatment of early gastric cancer in the elderly: overall survival, safety, and technical success

    Moreno-Sanchez, M. / De Tejada, A. Herreros / Fernandez-Esparrach, G. / Goikoetxea, U. / De Santiago, E. Rodriguez / Albéniz, E. / Sánchez, J. Rodríguez / Miranda, P. / Rincón, O. Nogales / Uchima, H. / Terán, Á. / Marín-Gabriel, J. C.

    Endoscopy

    2024  Volume 56, Issue S 02

    Event/congress ESGE Days 2024, Berlin, Germany, 2024-04-25
    Language English
    Publishing date 2024-04-01
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 80120-3
    ISSN 1438-8812 ; 0013-726X
    ISSN (online) 1438-8812
    ISSN 0013-726X
    DOI 10.1055/s-0044-1782841
    Database Thieme publisher's database

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  4. Article ; Online: CTNNB1 somatic mutations drive Wnt pathway activation in a case of incidental intranodal palisaded myofibroblastoma.

    Moreno de Juan, Germán / Montes Moreno, Santiago

    Revista espanola de patologia : publicacion oficial de la Sociedad Espanola de Anatomia Patologica y de la Sociedad Espanola de Citologia

    2023  Volume 56, Issue 4, Page(s) 279–283

    Abstract: Intranodal palisaded myofibroblastoma (IPM) is a rare stroma-derived spindle-cell neoplasm of the lymph node with myofibroblastic differentiation and CTNNB1 (β-catenin gene) somatic mutations. We present a case of IPM found incidentally in the staging of ...

    Abstract Intranodal palisaded myofibroblastoma (IPM) is a rare stroma-derived spindle-cell neoplasm of the lymph node with myofibroblastic differentiation and CTNNB1 (β-catenin gene) somatic mutations. We present a case of IPM found incidentally in the staging of lung adenocarcinoma. We describe the major histopathological and phenotypic features, including a palisaded bland spindle cell proliferation with myofibroblastic differentiation and Wnt pathway activation by immunohistochemistry, including β-catenin expression. Production of osteoid-like collagen directly from tumor cells was observed. We confirmed p.Gly34Arg CTNNB1 mutation by direct sequencing. We also reviewed the literature for similar cases.
    MeSH term(s) Humans ; beta Catenin/genetics ; beta Catenin/metabolism ; Wnt Signaling Pathway/genetics ; Lymph Nodes/pathology ; Neoplasms, Muscle Tissue/genetics ; Neoplasms, Muscle Tissue/metabolism ; Neoplasms, Muscle Tissue/pathology ; Mutation
    Chemical Substances beta Catenin ; CTNNB1 protein, human
    Language English
    Publishing date 2023-05-01
    Publishing country Spain
    Document type Case Reports
    ZDB-ID 2463888-2
    ISSN 1988-561X ; 1988-561X
    ISSN (online) 1988-561X
    ISSN 1988-561X
    DOI 10.1016/j.patol.2023.02.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A Circular Economy Approach to Integrate Divergent Ruminant Production Systems: Using Dairy Cow Feed Leftovers to Enhance the Out-of-Season Reproductive Performance in Goats.

    Machado-Ramos, Maria G / Meza-Herrera, Cesar A / De Santiago-Miramontes, Angeles / Mellado, Miguel / Véliz-Deras, Francisco G / Arellano-Rodríguez, Fernando / Contreras-Villarreal, Viridiana / Arévalo, José R / Carrillo-Moreno, Dalia I / Flores-Salas, Jessica M

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 15

    Abstract: Based on a circular economy approach, we evaluated the possible effect of targeted supplementation with leftover feed from dairy cows (i.e., intensive system) on the reproductive performance of crossbred/rangeland goats (i.e., extensive system) in arid ... ...

    Abstract Based on a circular economy approach, we evaluated the possible effect of targeted supplementation with leftover feed from dairy cows (i.e., intensive system) on the reproductive performance of crossbred/rangeland goats (i.e., extensive system) in arid Northern Mexico. During the deep-anestrous season (i.e., March-April, 25° North), multiparous goats (
    Language English
    Publishing date 2023-07-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13152431
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  6. Article: The price of innovation - the role of drug pricing in financing pharmaceutical innovation. A conceptual framework.

    Moreno, Santiago G / Epstein, David

    Journal of market access & health policy

    2019  Volume 7, Issue 1, Page(s) 1583536

    Abstract: The debate on drug prices has reached new heights with the controversy around the role of prices in promoting innovation. Critics claim that prices of innovative drugs are excessive and argue that lowering prices will not harm the flourishing innovation. ...

    Abstract The debate on drug prices has reached new heights with the controversy around the role of prices in promoting innovation. Critics claim that prices of innovative drugs are excessive and argue that lowering prices will not harm the flourishing innovation. On the opposite end, the pharmaceutical industry insists that restrictive pricing policies will have a detrimental impact on their ability to generate innovation. Amid these two divergent positions, this manuscript presents a conceptual framework to better understand the role played by drug prices to influence the ability of pharmaceutical firms to raise money in capital markets and hence finance pharmaceutical innovation. We argue that deviations from established value-based pricing principles, by either firms or payers, will distort access by firms to capital and lead to an undesirable level of innovation in the long term. We hope that this framework helps policy-makers anticipate the impact of their proposals, and ultimately guide policies towards setting optimal drug prices as a means to maximise social welfare.
    Language English
    Publishing date 2019-03-20
    Publishing country United States
    Document type Journal Article
    ISSN 2001-6689
    ISSN 2001-6689
    DOI 10.1080/20016689.2019.1583536
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  7. Article ; Online: The price of innovation - the role of drug pricing in financing pharmaceutical innovation. A conceptual framework

    Santiago G. Moreno / David Epstein

    Journal of Market Access & Health Policy, Vol 7, Iss

    2019  Volume 1

    Abstract: The debate on drug prices has reached new heights with the controversy around the role of prices in promoting innovation. Critics claim that prices of innovative drugs are excessive and argue that lowering prices will not harm the flourishing innovation. ...

    Abstract The debate on drug prices has reached new heights with the controversy around the role of prices in promoting innovation. Critics claim that prices of innovative drugs are excessive and argue that lowering prices will not harm the flourishing innovation. On the opposite end, the pharmaceutical industry insists that restrictive pricing policies will have a detrimental impact on their ability to generate innovation. Amid these two divergent positions, this manuscript presents a conceptual framework to better understand the role played by drug prices to influence the ability of pharmaceutical firms to raise money in capital markets and hence finance pharmaceutical innovation. We argue that deviations from established value-based pricing principles, by either firms or payers, will distort access by firms to capital and lead to an undesirable level of innovation in the long term. We hope that this framework helps policy-makers anticipate the impact of their proposals, and ultimately guide policies towards setting optimal drug prices as a means to maximise social welfare.
    Keywords innovation ; pharmaceutical industry ; health technology assessment (hta) ; finance ; value–based pricing ; Public aspects of medicine ; RA1-1270 ; Business ; HF5001-6182
    Subject code 330
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Execution of new trajectories toward a stable goal without a functional hippocampus.

    Duszkiewicz, Adrian J / Rossato, Janine I / Moreno, Andrea / Takeuchi, Tomonori / Yamasaki, Miwako / Genzel, Lisa / Spooner, Patrick / Canals, Santiago / Morris, Richard G M

    Hippocampus

    2023  Volume 33, Issue 6, Page(s) 769–786

    Abstract: The hippocampus is a critical component of a mammalian spatial navigation system, with the firing sequences of hippocampal place cells during sleep or immobility constituting a "replay" of an animal's past trajectories. A novel spatial navigation task ... ...

    Abstract The hippocampus is a critical component of a mammalian spatial navigation system, with the firing sequences of hippocampal place cells during sleep or immobility constituting a "replay" of an animal's past trajectories. A novel spatial navigation task recently revealed that such "replay" sequences of place fields can also prospectively map onto imminent new paths to a goal that occupies a stable location during each session. It was hypothesized that such "prospective replay" sequences may play a causal role in goal-directed navigation. In the present study, we query this putative causal role in finding only minimal effects of muscimol-induced inactivation of the dorsal and intermediate hippocampus on the same spatial navigation task. The concentration of muscimol used demonstrably inhibited hippocampal cell firing in vivo and caused a severe deficit in a hippocampal-dependent "episodic-like" spatial memory task in a watermaze. These findings call into question whether "prospective replay" of an imminent and direct path is actually necessary for its execution in certain navigational tasks.
    MeSH term(s) Animals ; Goals ; Muscimol/pharmacology ; Prospective Studies ; Spatial Navigation/physiology ; Hippocampus/physiology ; Mammals
    Chemical Substances Muscimol (2763-96-4)
    Language English
    Publishing date 2023-02-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1074352-2
    ISSN 1098-1063 ; 1050-9631
    ISSN (online) 1098-1063
    ISSN 1050-9631
    DOI 10.1002/hipo.23497
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3227: Show issues Location:
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  9. Article: Patient Experience of Lichen Planus: A Qualitative Exploration of Signs, Symptoms, and Health-Related Quality of Life Impacts.

    Mahon-Smith, Aoife / Clifford, Molly / Batish, Anjali / Sharp, Rosie / Panter, Charlotte / Naujoks, Christel / Schruf, Eva / Compagno, Nicolò / Moreno, Santiago G

    Dermatology and therapy

    2023  Volume 13, Issue 9, Page(s) 2001–2017

    Abstract: Introduction: Lichen planus (LP) is an inflammatory skin disorder that can present in various forms across the body, including lesions on the skin (cutaneous LP [CLP]), scalp (lichen planopilaris [LPP]), and mucosal regions (mucosal LP [MLP]). ... ...

    Abstract Introduction: Lichen planus (LP) is an inflammatory skin disorder that can present in various forms across the body, including lesions on the skin (cutaneous LP [CLP]), scalp (lichen planopilaris [LPP]), and mucosal regions (mucosal LP [MLP]). Qualitative exploration of the patient experience of LP, notably symptoms and impacts on health-related quality of life (HRQoL), is limited. A scarcity of research was also identified relating to emotional wellbeing impacts of CLP patients. Two qualitative studies were conducted with LP patients to address these gaps.
    Methods: Study 1 consisted of exit interviews conducted with a subset of adult patients with MLP (n = 5), CLP (n = 4), and LPP (n = 4) enrolled in an LP clinical study in the United States (US) to explore the patient experience. Study 2 consisted of independent qualitative interviews conducted with adult CLP patients (n = 13) from the US and Germany to further explore impacts on emotional wellbeing.
    Results: Exit interviews found that itch , pain, and skin lesions were most frequently reported as signs/symptoms of LP. Itch and skin lesions were experienced across all LP subtypes, while pain was only reported by CLP and MLP patients. These signs/symptoms impacted HRQoL including emotional wellbeing (frustration, embarrassment), daily activities (oral hygiene, clothing options), social functioning (intimacy, social activities), and physical functioning (chewing/swallowing, opening/moving mouth). Impacts on activities of daily living (ADL) and physical functioning were mostly experienced by MLP patients. Independent qualitative interviews, which further explored impacts of CLP on patients' emotional wellbeing, identified frustration, worry, sadness, embarrassment, and depression as the most frequently experienced.
    Conclusion: The findings contribute to the literature by providing qualitative insights into signs/symptoms and HRQoL impacts of LP, from the adult patient perspective. The findings also highlight the importance of considering assessment of HRQoL impacts in future clinical LP research, particularly impacts on emotional wellbeing when selecting instruments for assessment of HRQoL in the CLP population.
    Trial registration: NCT04300296.
    Language English
    Publishing date 2023-07-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2680284-3
    ISSN 2190-9172 ; 2193-8210
    ISSN (online) 2190-9172
    ISSN 2193-8210
    DOI 10.1007/s13555-023-00968-2
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  10. Article ; Online: Letter by moreno regarding article, "cost-effectiveness analysis of continuous flow left ventricular assist devices as destination therapy".

    Moreno, Santiago G

    Circulation. Heart failure

    2012  Volume 5, Issue 2, Page(s) e50

    MeSH term(s) Heart Failure/economics ; Heart Failure/therapy ; Heart-Assist Devices/economics ; Humans
    Language English
    Publishing date 2012-03-01
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 2429459-7
    ISSN 1941-3297 ; 1941-3289
    ISSN (online) 1941-3297
    ISSN 1941-3289
    DOI 10.1161/CIRCHEARTFAILURE.111.966085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6745: Show issues Location:
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