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  1. Article ; Online: Interaction of Positively Charged Oligopeptides with Blood Plasma Proteins.

    Kotynia, Aleksandra / Marciniak, Aleksandra / Kamysz, Wojciech / Neubauer, Damian / Krzyżak, Edward

    International journal of molecular sciences

    2023  Volume 24, Issue 3

    Abstract: In this project, we combine two areas of research, experimental characterization and molecular docking studies of the interaction of positively charged oligopeptides with crucial blood plasma proteins. The investigated peptides are rich in ... ...

    Abstract In this project, we combine two areas of research, experimental characterization and molecular docking studies of the interaction of positively charged oligopeptides with crucial blood plasma proteins. The investigated peptides are rich in NH
    MeSH term(s) Amino Acid Sequence ; Molecular Docking Simulation ; Peptides/chemistry ; Oligopeptides/chemistry ; Lysine/chemistry ; Blood Proteins ; Albumins
    Chemical Substances Peptides ; Oligopeptides ; Lysine (K3Z4F929H6) ; Blood Proteins ; Albumins
    Language English
    Publishing date 2023-02-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032836
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  2. Article ; Online: Glycosylated Lipopeptides-Synthesis and Evaluation of Antimicrobial Activity and Cytotoxicity.

    Sikora, Karol / Bauer, Marta / Bartoszewska, Sylwia / Neubauer, Damian / Kamysz, Wojciech

    Biomolecules

    2023  Volume 13, Issue 1

    Abstract: Ultrashort cationic lipopeptides (USCLs) are promising antimicrobial agents that may be used to combat pathogens such as bacteria and fungi. USCLs consist of a few basic amino acid residues and at least one lipid moiety, usually a fatty acid chain. ... ...

    Abstract Ultrashort cationic lipopeptides (USCLs) are promising antimicrobial agents that may be used to combat pathogens such as bacteria and fungi. USCLs consist of a few basic amino acid residues and at least one lipid moiety, usually a fatty acid chain. Generally, USCLs are potent antimicrobials but their major shortcoming is a relatively high cytotoxicity and hemolytic activity. Glycopeptide antibiotics (e.g. vancomycin) are essential in combating bacterial infections and are popular in medicinal practice. However, literature concerning the effect of glycosylation of peptides on their antimicrobial activity is rather scarce. For the first time, this study highlights the effect of USCLs glycosylation on in vitro biological activity. The aim of this study was to evaluate the impact of glycosylation of a series of USCLs on antimicrobial activity, cytotoxicity and hemolytic activity. Straight-chain fatty acids (C14, C16, C18) were attached to the
    MeSH term(s) Humans ; Lipopeptides/pharmacology ; Lipopeptides/chemistry ; Glycosylation ; Staphylococcus aureus ; Microbial Sensitivity Tests ; Anti-Infective Agents/pharmacology ; Anti-Infective Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Fatty Acids/chemistry ; Serine
    Chemical Substances Lipopeptides ; Anti-Infective Agents ; Anti-Bacterial Agents ; Fatty Acids ; Serine (452VLY9402)
    Language English
    Publishing date 2023-01-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom13010172
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  3. Article ; Online: Evaluation of Linkers' Influence on Peptide-Based Piezoelectric Biosensors' Sensitivity to Aldehydes in the Gas Phase.

    Wasilewski, Tomasz / Neubauer, Damian / Wojciechowski, Marek / Szulczyński, Bartosz / Gębicki, Jacek / Kamysz, Wojciech

    International journal of molecular sciences

    2023  Volume 24, Issue 13

    Abstract: Recent findings qualified aldehydes as potential biomarkers for disease diagnosis. One of the possibilities is to use electrochemical biosensors in point-of-care (PoC), but these need further development to overcome some limitations. Currently, the ... ...

    Abstract Recent findings qualified aldehydes as potential biomarkers for disease diagnosis. One of the possibilities is to use electrochemical biosensors in point-of-care (PoC), but these need further development to overcome some limitations. Currently, the primary goal is to enhance their metrological parameters in terms of sensitivity and selectivity. Previous findings indicate that peptide OBPP4 (KLLFDSLTDLKKKMSEC-NH
    MeSH term(s) Peptides/chemistry ; Aldehydes/chemistry ; Amino Acids/chemistry ; Biosensing Techniques
    Chemical Substances nonanal (2L2WBY9K6T) ; Peptides ; Aldehydes ; Amino Acids
    Language English
    Publishing date 2023-06-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241310610
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Interaction of Positively Charged Oligopeptides with Blood Plasma Proteins

    Aleksandra Kotynia / Aleksandra Marciniak / Wojciech Kamysz / Damian Neubauer / Edward Krzyżak

    International Journal of Molecular Sciences, Vol 24, Iss 2836, p

    2023  Volume 2836

    Abstract: In this project, we combine two areas of research, experimental characterization and molecular docking studies of the interaction of positively charged oligopeptides with crucial blood plasma proteins. The investigated peptides are rich in NH 2 groups of ...

    Abstract In this project, we combine two areas of research, experimental characterization and molecular docking studies of the interaction of positively charged oligopeptides with crucial blood plasma proteins. The investigated peptides are rich in NH 2 groups of amino acid side chains from Dap, Orn, Lys, and Arg residues, which are relevant in protein interaction. The peptides are 9- and 11-mer with the following sequences: (Lys-Dab-Dab-Gly-Orn-Pro-His-Lys-Arg-Lys-Dbt), (Lys-Dab-Ala-Gly-Orn-Pro-His-Lys-Arg), and (Lys-Dab-Dab-Gly-Orn-Pro-Phe(2-F)-Lys-Arg). The net charge of the compound strongly depends on the pH environment and it is an important aspect of protein binding. The studied oligopeptides exhibit therapeutic properties: anti-inflammatory activity and the capacity to diminish reactive oxygen species (ROS). Therefore, the mechanism of potential binding with blood plasma components is the next challenge. The binding interaction has been investigated under pseudo-physiological conditions with the main blood plasma proteins: albumin (BSA), α1-acid glycoprotein (AAG), and γ-globulin fraction (GGF). The biomolecular quenching constant (k q ) and binding constant (K b ) were obtained by fluorescence spectroscopy at various temperatures. Simultaneously, the changes in the secondary structure of proteins were monitored by circular dichroism (CD) and infrared spectroscopy (IR) by quantity analysis. Moreover, molecular docking studies were conducted to estimate the binding affinity, the binding domain, and the chemical nature of these interactions. The results show that the investigated oligopeptides could be mainly transported by albumin, and the binding domain I is the most favored cavity. The BSA and GGF are able to form stable complexes with the studied compounds as opposed to AAG. The binding reactions are spontaneous processes. The highest binding constants were determined for Lys-Dab-Dab-Gly-Orn-Pro-His-Lys-Arg-Lys-Dbt peptide, in which the values of the binding constants K b to BSA and GGF were 10.1 × 10 4 dm 3 ...
    Keywords oligopeptide ; bovine serum albumin ; α-acid glycoprotein ; γ-globulin ; quenching constant (k q ) ; binding constant (K b ) ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: The Study of Antistaphylococcal Potential of Omiganan and Retro-Omiganan Under Flow Conditions.

    Jaśkiewicz, Maciej / Neubauer, Damian / Sikora, Karol / Bauer, Marta / Bartoszewska, Sylwia / Błażewicz, Izabela / Marek, Dariusz / Barańska-Rybak, Wioletta / Kamysz, Wojciech

    Probiotics and antimicrobial proteins

    2024  

    Abstract: Staphylococcus aureus is considered one of the leading pathogens responsible for infections in humans and animals. The heterogeneous nature of diseases caused by these bacteria is due to the occurrence of multiple strains, differentiated by several ... ...

    Abstract Staphylococcus aureus is considered one of the leading pathogens responsible for infections in humans and animals. The heterogeneous nature of diseases caused by these bacteria is due to the occurrence of multiple strains, differentiated by several mechanisms of antibiotic resistance and virulence factors. One of these is the ability to form biofilm. Biofilm-associated bacteria exhibit a different phenotype that protects them from external factors such as the activity of immune system or antimicrobial substances. Moreover, it has been shown that the majority of persistent and recurrent infections are associated with the presence of the biofilm. Omiganan, an analog of indolicidin - antimicrobial peptide (AMP) derived from bovine neutrophil granules, was found to exhibit high antistaphylococcal and antibiofilm potential. Furthermore, its analog with a reversed sequence (retro-omiganan) was found to display enhanced activity against a variety of pathogens. Based on experience of our group, we found out that counterion exchange can improve the antistaphylococcal activity of AMPs. The aim of this study was to investigate the activity of both compounds against S. aureus biofilm under flow conditions. The advantage of this approach was that it offered the opportunity to form and characterize the biofilm under more controlled conditions. To do this, unique flow cells made of polydimethylsiloxane (PDMS) were developed. The activity against pre-formed biofilm as well as AMPs-treated bacteria was measured. Also, the incorporation of omiganan and retro-omiganan into the channels was conducted to learn whether or not it would inhibit the development of biofilm. The results of the microbiological tests ultimately confirmed the high potential of the omiganan and its retro-analog as well as the importance of counterion exchange in terms of antimicrobial examination. We found out that retro-omiganan trifluoroacetate had the highest biofilm inhibitory properties, however, acetates of both compounds exhibited the highest activity against planktonic and biofilm cultures. Moreover, the developed methodology of investigation under flow conditions allows the implementation of the studies under flow conditions to other compounds.
    Language English
    Publishing date 2024-01-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2487792-X
    ISSN 1867-1314 ; 1867-1306
    ISSN (online) 1867-1314
    ISSN 1867-1306
    DOI 10.1007/s12602-023-10197-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Antifungal Activity of Linear and Disulfide-Cyclized Ultrashort Cationic Lipopeptides Alone and in Combination with Fluconazole against Vulvovaginal

    Czechowicz, Paulina / Neubauer, Damian / Nowicka, Joanna / Kamysz, Wojciech / Gościniak, Grażyna

    Pharmaceutics

    2021  Volume 13, Issue 10

    Abstract: Vulvovaginal candidiasis (VVC) occurs in over 75% of women at least once during their lifetime and is an infection that significantly affects their health. ...

    Abstract Vulvovaginal candidiasis (VVC) occurs in over 75% of women at least once during their lifetime and is an infection that significantly affects their health.
    Language English
    Publishing date 2021-09-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics13101589
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  7. Article: New Teixobactin Analogues with a Total Lactam Ring.

    Scioli, Giuseppe / Marinaccio, Lorenza / Bauer, Marta / Kamysz, Wojciech / Parmar, Anish / Newire, Enas / Singh, Ishwar / Stefanucci, Azzurra / Mollica, Adriano

    ACS medicinal chemistry letters

    2023  Volume 14, Issue 12, Page(s) 1827–1832

    Abstract: Teixobactin is a new antibiotic peptide with strong efficacy against several Gram-positive resistant bacteria, the structure of which is extremely difficult to obtain in the laboratory via multistep conventional synthesis. To face the increasing ... ...

    Abstract Teixobactin is a new antibiotic peptide with strong efficacy against several Gram-positive resistant bacteria, the structure of which is extremely difficult to obtain in the laboratory via multistep conventional synthesis. To face the increasing antibiotic resistant bacteria, it is fundamental to introduce new types of antibiotics with innovative mechanisms of action without resistance; thus, many scientists are studying and developing new methods to synthesize teixobactin analogues. In this work, seven Arg
    Language English
    Publishing date 2023-11-14
    Publishing country United States
    Document type Journal Article
    ISSN 1948-5875
    ISSN 1948-5875
    DOI 10.1021/acsmedchemlett.3c00435
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  8. Article ; Online: Evaluation of Linkers’ Influence on Peptide-Based Piezoelectric Biosensors’ Sensitivity to Aldehydes in the Gas Phase

    Tomasz Wasilewski / Damian Neubauer / Marek Wojciechowski / Bartosz Szulczyński / Jacek Gębicki / Wojciech Kamysz

    International Journal of Molecular Sciences, Vol 24, Iss 10610, p

    2023  Volume 10610

    Abstract: Recent findings qualified aldehydes as potential biomarkers for disease diagnosis. One of the possibilities is to use electrochemical biosensors in point-of-care (PoC), but these need further development to overcome some limitations. Currently, the ... ...

    Abstract Recent findings qualified aldehydes as potential biomarkers for disease diagnosis. One of the possibilities is to use electrochemical biosensors in point-of-care (PoC), but these need further development to overcome some limitations. Currently, the primary goal is to enhance their metrological parameters in terms of sensitivity and selectivity. Previous findings indicate that peptide OBPP4 (KLLFDSLTDLKKKMSEC-NH 2 ) is a promising candidate for further development of aldehyde-sensitive biosensors. To increase the affinity of a receptor layer to long-chain aldehydes, a structure stabilization of the peptide active site via the incorporation of different linkers was studied. Indeed, the incorporation of linkers improved sensitivity to and binding of aldehydes in comparison to that of the original peptide-based biosensor. The tendency to adopt disordered structures was diminished owing to the implementation of suitable linkers. Therefore, to improve the metrological characteristics of peptide-based piezoelectric biosensors, linkers were added at the C-terminus of OBPP4 peptide (KLLFDSLTDLKKKMSE-linker-C-NH 2 ). Those linkers consist of proteinogenic amino acids from group one: glycine, L-proline, L-serine, and non proteinogenic amino acids from group two: β-alanine, 4-aminobutyric acid, and 6-aminohexanoic acid. Linkers were evaluated with in silico studies, followed by experimental verification. All studied linkers enhanced the detection of aldehydes in the gas phase. The highest difference in frequency (60 Hz, nonanal) was observed between original peptide-based biosensors and ones based on peptides modified with the GSGSGS linker. It allowed evaluation of the limit of detection for nonanal at the level of 2 ppm, which is nine times lower than that of the original peptide. The highest sensitivity values were also obtained for the GSGSGS linker: 0.3312, 0.4281, and 0.4676 Hz/ppm for pentanal, octanal, and nonanal, respectively. An order of magnitude increase in sensitivity was observed for the six linkers used. ...
    Keywords sensors ; biosensors ; QCM ; peptides ; aldehydes ; linkers ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
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  9. Article: Methods Used for the Eradication of Staphylococcal Biofilms.

    Jaśkiewicz, Maciej / Janczura, Adriana / Nowicka, Joanna / Kamysz, Wojciech

    Antibiotics (Basel, Switzerland)

    2019  Volume 8, Issue 4

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus aureus
    Language English
    Publishing date 2019-10-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics8040174
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications.

    Wasilewski, Tomasz / Szulczyński, Bartosz / Dobrzyniewski, Dominik / Jakubaszek, Weronika / Gębicki, Jacek / Kamysz, Wojciech

    Biosensors

    2022  Volume 12, Issue 5

    Abstract: Cleaning a quartz crystal microbalance (QCM) plays a crucial role in the regeneration of its biosensors for reuse. Imprecise removal of a receptor layer from a transducer's surface can lead to unsteady operation during measurements. This article compares ...

    Abstract Cleaning a quartz crystal microbalance (QCM) plays a crucial role in the regeneration of its biosensors for reuse. Imprecise removal of a receptor layer from a transducer's surface can lead to unsteady operation during measurements. This article compares three approaches to regeneration of the piezoelectric transducers using the electrochemical, oxygen plasma and Piranha solution methods. Optimization of the cleaning method allowed for evaluation of the influence of cleaning on the surface of regenerated biosensors. The effectiveness of cleaning the QCM transducers with a receptor layer in the form of a peptide with the KLLFDSLTDLKKKMSEC-NH
    MeSH term(s) Biosensing Techniques/methods ; Electrodes ; Peptides ; Quartz/chemistry ; Quartz Crystal Microbalance Techniques
    Chemical Substances Peptides ; Quartz (14808-60-7)
    Language English
    Publishing date 2022-05-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios12050309
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