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Article ; Online: Circular dichroism assessment of an imidazole antifungal drug with plant based silver nanoparticles: Quantitative and DFT analysis.

Khalil, Adila / Kashif, Mohammad

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2023 Volume 296, Page(s) 122638

Abstract: Circular dichroism (CD) methods have been developed for the analysis of luliconazole (LUC) using plant based silver nanoparticles (P-AgNPs). Cleaner and natural approach have found significant attention in recent times owing to their exceptional ... ...

Abstract	Circular dichroism (CD) methods have been developed for the analysis of luliconazole (LUC) using plant based silver nanoparticles (P-AgNPs). Cleaner and natural approach have found significant attention in recent times owing to their exceptional physicochemical characteristics. Utilizing FTIR, SEM, and XRD, the produced nanoparticles were analyzed. The produced P-AgNPs were then used to assay LUC in formulation drugs. Four CD methods are developed as zero order and second order derivative methods. Methods I and II are based on a normal CD scan (zero order) that produced calibration range from 2 - 16 μgmL
MeSH term(s)	Metal Nanoparticles/chemistry ; Silver/chemistry ; Antifungal Agents ; Circular Dichroism ; Imidazoles ; Plant Extracts/chemistry ; Anti-Bacterial Agents/chemistry
Chemical Substances	Silver (3M4G523W1G) ; Antifungal Agents ; Imidazoles ; Plant Extracts ; Anti-Bacterial Agents
Language	English
Publishing date	2023-03-21
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2023.122638
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Article ; Online: Molecular Interactions Leading to the Advancement in the Techniques for COVID-19 Detection: A Review.

Kashif, Mohammad / Acharya, Swati / Khalil, Adila

Journal of AOAC International

2024

Abstract: Since 2019 the world is in a combat with the highly contagious disease COVID-19 that is caused by the rapid transmission of SARS-CoV-2 virus (severe acute respiratory syndrome- coronavirus-2). Detection of this disease in an early stage helps to control ... ...

Abstract	Since 2019 the world is in a combat with the highly contagious disease COVID-19 that is caused by the rapid transmission of SARS-CoV-2 virus (severe acute respiratory syndrome- coronavirus-2). Detection of this disease in an early stage helps to control its spread and management. To combat this epidemic with one-time effective medication improved quick analytical procedures must be developed and validated. The requirement of accurate and precise analytical methods for the diagnosis of the virus and antibodies in infected patients has been a matter of concern. The global impact of this virus has motivated scientists and researchers to investigate and develop various analytical diagnostic techniques. This review includes the study of standard methods which are reliable and accredited for the analytical recognition of the said virus. For early detection of SARS-CoV-2 RNA, RT-PCR is an accurate method among other methods and thus, considered as "gold standard" technique. Here, we outline the most extensively used analytical methods for diagnosing COVID-19, along with a brief description of each technique and its analytical aspects/perspective.
Language	English
Publishing date	2024-02-03
Publishing country	England
Document type	Journal Article
ZDB-ID	1103149-9
ISSN	1944-7922 ; 1060-3271
ISSN (online)	1944-7922
ISSN	1060-3271
DOI	10.1093/jaoacint/qsae010
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Article ; Online: Development of UV-visible spectrophotometric methods for the quantitative and

Khalil, Adila / Kashif, Mohammad

Drug development and industrial pharmacy

2021 Volume 47, Issue 7, Page(s) 1100–1111

Abstract: For cilazapril (CLZ), analytical methods based on donor-acceptor phenomenon that are simple, rapid with broad linear dynamic range for the quantification of drug are not available in the literature. Considering the requirement for the methods, in this ... ...

Abstract	For cilazapril (CLZ), analytical methods based on donor-acceptor phenomenon that are simple, rapid with broad linear dynamic range for the quantification of drug are not available in the literature. Considering the requirement for the methods, in this study, two economic, potent analytical methods based on the complexation of CLZ with π-acceptors, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (CA) were developed, validated, and studied spectrophotometrically. Various analytical data were discussed. The effects of experimental variables were optimized from the results of
MeSH term(s)	Benzoquinones ; Cilazapril ; Molecular Docking Simulation ; Spectrophotometry
Chemical Substances	Benzoquinones ; Cilazapril (19KW7PI29F)
Language	English
Publishing date	2021-08-06
Publishing country	England
Document type	Journal Article
ZDB-ID	751874-2
ISSN	1520-5762 ; 0363-9045
ISSN (online)	1520-5762
ISSN	0363-9045
DOI	10.1080/03639045.2021.1957918
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Article ; Online: Pharmacogenetic study of CES1 gene and enalapril efficacy.

Hussain, Misbah / Basheer, Sehrish / Khalil, Adila / Haider, Qurat Ul Ain / Saeed, Hafsa / Faizan, Muhammad

Journal of applied genetics

2024

Abstract: Enalapril is an orally administered angiotensin-converting enzyme inhibitor which is widely prescribed to treat hypertension, chronic kidney disease, and heart failure. It is an ester prodrug that needs to be activated by carboxylesterase 1 (CES1). CES1 ... ...

Abstract	Enalapril is an orally administered angiotensin-converting enzyme inhibitor which is widely prescribed to treat hypertension, chronic kidney disease, and heart failure. It is an ester prodrug that needs to be activated by carboxylesterase 1 (CES1). CES1 is a hepatic hydrolase that in vivo biotransforms enalapril to its active form enalaprilat in order to produce its desired pharmacological impact. Several single nucleotide polymorphisms in CES1 gene are reported to alter the catalytic activity of CES1 enzyme and influence enalapril metabolism. G143E, L40T, G142E, G147C, Y170D, and R171C can completely block the enalapril metabolism. Some polymorphisms like Q169P, E220G, and D269fs do not completely block the CES1 function; however, they reduce the catalytic activity of CES1 enzyme. The prevalence of these polymorphisms is not the same among all populations which necessitate to consider the genetic panel of respective population before prescribing enalapril. These genetic variations are also responsible for interindividual variability of CES1 enzyme activity which ultimately affects the pharmacokinetics and pharmacodynamics of enalapril. The current review summarizes the CES1 polymorphisms which influence the enalapril metabolism and efficacy. The structure of CES1 catalytic domain and important amino acids impacting the catalytic activity of CES1 enzyme are also discussed. This review also highlights the importance of pharmacogenomics in personalized medicine.
Language	English
Publishing date	2024-01-23
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1235302-4
ISSN	2190-3883 ; 1234-1983
ISSN (online)	2190-3883
ISSN	1234-1983
DOI	10.1007/s13353-024-00831-w
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Article ; Online: Nuclear Magnetic Resonance Spectroscopy for Quantitative Analysis: A Review for Its Application in the Chemical, Pharmaceutical and Medicinal Domains.

Khalil, Adila / Kashif, Mohammad

Critical reviews in analytical chemistry

2021 Volume 53, Issue 5, Page(s) 997–1011

Abstract: Nuclear magnetic resonance (NMR) is a rapid and accurate analytical tool for qualification and quantification. The capacity of NMR of being quantitative can also justify the calibration of other analytical methods. In pharmaceutical domain, quantitative ... ...

Abstract	Nuclear magnetic resonance (NMR) is a rapid and accurate analytical tool for qualification and quantification. The capacity of NMR of being quantitative can also justify the calibration of other analytical methods. In pharmaceutical domain, quantitative NMR (qNMR) can be applied in the identification and quantification of drug simultaneously. The early drug development stage requires a minimum sample for analysis. Thus, priority should be given to utilize this technique to attain results with least investment, rapid analysis time and minimum sample consumption. This technique is a significant phenomenon to identify impurities, drug substance, residual solvents of in-process control (IPC) samples and characterizing the formulations. From an analyst's perspective, qNMR proved to be a routine practice in pharmaceutical industry to qualify any drug product. The absolute and relative methods offer great help in quantifying the component of interest in the process control samples and finished products. This review highlights the evolution of NMR application in the pharmaceutical industry, where determining the purity of drug substance, drug product and establishing the identity of impurities and its level are the challenging aspects. NMR in medicinal field emerging as a numero uno for Covid-19 severity detection and its dire consequences, accelerated vaccine development and the mapping of SAR-COV-2 RNA and proteins via chemical shift assignments.
MeSH term(s)	Humans ; COVID-19 ; Magnetic Resonance Spectroscopy/methods ; Drug Compounding ; Pharmaceutical Preparations
Chemical Substances	Pharmaceutical Preparations
Language	English
Publishing date	2021-11-09
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2030019-0
ISSN	1547-6510 ; 1040-8347
ISSN (online)	1547-6510
ISSN	1040-8347
DOI	10.1080/10408347.2021.2000359
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Article ; Online: Development of low-cost in-house tetra-ARMS-PCR assay for the screening of five

Khalil, Adila / Khan, Haq Nawaz / Wasim, Muhammad / Ayesha, Hina / Awan, Fazli Rabbi

Nucleosides, nucleotides & nucleic acids

2023 , Page(s) 1–13

Abstract: Background: Classical homocystinuria is an inborn amino acid metabolism disorder resulting from mutations in the Cystathionine-β-Synthase (CBS) gene. These mutations lead to elevated homocysteine and methionine levels and reduced cysteine levels in the ... ...

Abstract	Background: Classical homocystinuria is an inborn amino acid metabolism disorder resulting from mutations in the Cystathionine-β-Synthase (CBS) gene. These mutations lead to elevated homocysteine and methionine levels and reduced cysteine levels in the blood. Typically, diagnosis occurs after patients display symptoms, and various lab methods confirm it. DNA sequencing is the best option for early detection of genetic variants in asymptomatic suspected individuals. Unfortunately, its high cost can hinder its use, especially in low-income countries like Pakistan. Objective: Aim of this study was to devise a robust low-cost diagnostic/screening assay based on Tetra-ARMS-PCR for five prevalent genetic variants found in Pakistani classical homocystinuria patients. Materials and methods: In the current study, T-ARMS-PCR assays were developed for five mutations (c.975G > C, c.770C > T, c.752T > C, c.1039 + 1G > T, c.451 + 1GG > TA), which were characterized previously in classical homocystinuria patients. These low-cost T-ARMS-PCR assays were then used to screen the affected individuals and their family members to identify their genotypes for pathogenic variations in the asymptomatic patients and carriers in their respective families. Results: The outcomes were entirely consistent with those obtained from Sanger DNA sequencing, confirming the sensitivity, specificity, and reliability of the T-ARMS-PCR assay for detecting Conclusion: T-ARMS-PCR has wide applications for low-income countries for the screening and early diagnosis of asymptomatic patients and carriers in the homocystinuria affected families as well as other inherited diseases.
Language	English
Publishing date	2023-11-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2008956-9
ISSN	1532-2335 ; 1525-7770
ISSN (online)	1532-2335
ISSN	1525-7770
DOI	10.1080/15257770.2023.2280013
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Article ; Online: Histological and Microscopic Analysis of Fats in Heart, Liver Tissue, and Blood Parameters in Experimental Mice.

Basheer, Sehrish / Malik, Imran Riaz / Awan, Fazli Rabbi / Sughra, Kalsoom / Roshan, Sadia / Khalil, Adila / Iqbal, Muhammad Javed / Parveen, Zahida

Genes

2023 Volume 14, Issue 2

Abstract: The intake of various types and amounts of dietary fats influences metabolic and cardiovascular health. Hence, this study evaluated the impact of routinely consumed Pakistani dietary fats on their cardiometabolic impact. For this, we made four groups of ... ...

Abstract	The intake of various types and amounts of dietary fats influences metabolic and cardiovascular health. Hence, this study evaluated the impact of routinely consumed Pakistani dietary fats on their cardiometabolic impact. For this, we made four groups of mice, each comprising 5 animals: (1) C-ND: Control mice on a normal diet, (2) HFD-DG: High-fat diet mice on a normal diet plus 10% (
MeSH term(s)	Mice ; Animals ; Ghee ; Liver/metabolism ; Body Weight ; Dietary Fats/metabolism ; Diet, High-Fat
Chemical Substances	Ghee ; Dietary Fats
Language	English
Publishing date	2023-02-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes14020515
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Article ; Online: Histological and Microscopic Analysis of Fats in Heart, Liver Tissue, and Blood Parameters in Experimental Mice

Basheer, Sehrish / Malik, Imran Riaz / Awan, Fazli Rabbi / Sughra, Kalsoom / Roshan, Sadia / Khalil, Adila / Iqbal, Muhammad Javed / Parveen, Zahida

Genes (Basel). 2023 Feb. 17, v. 14, no. 2

2023

Abstract	The intake of various types and amounts of dietary fats influences metabolic and cardiovascular health. Hence, this study evaluated the impact of routinely consumed Pakistani dietary fats on their cardiometabolic impact. For this, we made four groups of mice, each comprising 5 animals: (1) C-ND: Control mice on a normal diet, (2) HFD-DG: High-fat diet mice on a normal diet plus 10% (w/w) desi ghee, (3) HFD-O: Mice on normal diet plus 10% (w/w) plant oil (4) HFD-BG: Mice on normal diet plus 10% (w/w) banaspati ghee. Mice were fed for 16 weeks, and blood, liver, and heart samples were collected for biochemical, histological, and electron microscopic analysis. The physical factors indicated that mice fed on HFD gained more body weight than the C-ND group. Blood parameters do not show significant differences, but overall, the glucose and cholesterol concentrations were raised in the mice fed with a fat-rich diet, with the highest concentrations in the HFD-BG group. The mice fed with HFD-BG and HFD-O had more lipid droplets in the liver, compared to HFD-DG and C-ND.
Keywords	blood ; body weight ; cholesterol ; electron microscopy ; ghee ; glucose ; heart ; high fat diet ; histology ; liver ; plant fats and oils
Language	English
Dates of publication	2023-0217
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes14020515
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The landscape of genetic diseases in Saudi Arabia based on the first 1000 diagnostic panels and exomes.

Monies, Dorota / Abouelhoda, Mohamed / AlSayed, Moeenaldeen / Alhassnan, Zuhair / Alotaibi, Maha / Kayyali, Husam / Al-Owain, Mohammed / Shah, Ayaz / Rahbeeni, Zuhair / Al-Muhaizea, Mohammad A / Alzaidan, Hamad I / Cupler, Edward / Bohlega, Saeed / Faqeih, Eissa / Faden, Maha / Alyounes, Banan / Jaroudi, Dyala / Goljan, Ewa / Elbardisy, Hadeel /

Akilan, Asma / Albar, Renad / Aldhalaan, Hesham / Gulab, Shamshad / Chedrawi, Aziza / Al Saud, Bandar K / Kurdi, Wesam / Makhseed, Nawal / Alqasim, Tahani / El Khashab, Heba Y / Al-Mousa, Hamoud / Alhashem, Amal / Kanaan, Imaduddin / Algoufi, Talal / Alsaleem, Khalid / Basha, Talal A / Al-Murshedi, Fathiya / Khan, Sameena / Al-Kindy, Adila / Alnemer, Maha / Al-Hajjar, Sami / Alyamani, Suad / Aldhekri, Hasan / Al-Mehaidib, Ali / Arnaout, Rand / Dabbagh, Omar / Shagrani, Mohammad / Broering, Dieter / Tulbah, Maha / Alqassmi, Amal / Almugbel, Maisoon / AlQuaiz, Mohammed / Alsaman, Abdulaziz / Al-Thihli, Khalid / Sulaiman, Raashda A / Al-Dekhail, Wajeeh / Alsaegh, Abeer / Bashiri, Fahad A / Qari, Alya / Alhomadi, Suzan / Alkuraya, Hisham / Alsebayel, Mohammed / Hamad, Muddathir H / Szonyi, Laszlo / Abaalkhail, Faisal / Al-Mayouf, Sulaiman M / Almojalli, Hamad / Alqadi, Khalid S / Elsiesy, Hussien / Shuaib, Taghreed M / Seidahmed, Mohammed Zain / Abosoudah, Ibraheem / Akleh, Hana / AlGhonaium, Abdulaziz / Alkharfy, Turki M / Al Mutairi, Fuad / Eyaid, Wafa / Alshanbary, Abdullah / Sheikh, Farrukh R / Alsohaibani, Fahad I / Alsonbul, Abdullah / Al Tala, Saeed / Balkhy, Soher / Bassiouni, Randa / Alenizi, Ahmed S / Hussein, Maged H / Hassan, Saeed / Khalil, Mohamed / Tabarki, Brahim / Alshahwan, Saad / Oshi, Amira / Sabr, Yasser / Alsaadoun, Saad / Salih, Mustafa A / Mohamed, Sarar / Sultana, Habiba / Tamim, Abdullah / El-Haj, Moayad / Alshahrani, Saif / Bubshait, Dalal K / Alfadhel, Majid / Faquih, Tariq / El-Kalioby, Mohamed / Subhani, Shazia / Shah, Zeeshan / Moghrabi, Nabil / Meyer, Brian F / Alkuraya, Fowzan S

Human genetics

2017 Volume 136, Issue 8, Page(s) 921–939

Abstract: In this study, we report the experience of the only reference clinical next-generation sequencing lab in Saudi Arabia with the first 1000 families who span a wide-range of suspected Mendelian phenotypes. A total of 1019 tests were performed in the period ...

Abstract	In this study, we report the experience of the only reference clinical next-generation sequencing lab in Saudi Arabia with the first 1000 families who span a wide-range of suspected Mendelian phenotypes. A total of 1019 tests were performed in the period of March 2016-December 2016 comprising 972 solo (index only), 14 duo (parents or affected siblings only), and 33 trio (index and parents). Multigene panels accounted for 672 tests, while whole exome sequencing (WES) represented the remaining 347 tests. Pathogenic or likely pathogenic variants that explain the clinical indications were identified in 34% (27% in panels and 43% in exomes), spanning 279 genes and including 165 novel variants. While recessive mutations dominated the landscape of solved cases (71% of mutations, and 97% of which are homozygous), a substantial minority (27%) were solved on the basis of dominant mutations. The highly consanguineous nature of the study population also facilitated homozygosity for many private mutations (only 32.5% of the recessive mutations are founder), as well as the first instances of recessive inheritance of previously assumed strictly dominant disorders (involving ITPR1, VAMP1, MCTP2, and TBP). Surprisingly, however, dual molecular diagnosis was only observed in 1.5% of cases. Finally, we have encountered candidate variants in 75 genes (ABHD6, ACY3, ADGRB2, ADGRG7, AGTPBP1, AHNAK2, AKAP6, ASB3, ATXN1L, C17orf62, CABP1, CCDC186, CCP110, CLSTN2, CNTN3, CNTN5, CTNNA2, CWC22, DMAP1, DMKN, DMXL1, DSCAM, DVL2, ECI1, EP400, EPB41L5, FBXL22, GAP43, GEMIN7, GIT1, GRIK4, GRSF1, GTRP1, HID1, IFNL1, KCNC4, LRRC52, MAP7D3, MCTP2, MED26, MPP7, MRPS35, MTDH, MTMR9, NECAP2, NPAT, NRAP, PAX7, PCNX, PLCH2, PLEKHF1, PTPN12, QKI, RILPL2, RIMKLA, RIMS2, RNF213, ROBO1, SEC16A, SIAH1, SIRT2, SLAIN2, SLC22A20, SMDT1, SRRT, SSTR1, ST20, SYT9, TSPAN6, UBR4, VAMP4, VPS36, WDR59, WDYHV1, and WHSC1) not previously linked to human phenotypes and these are presented to accelerate post-publication matchmaking. Two of these genes were independently mutated in more than one family with similar phenotypes, which substantiates their link to human disease (AKAP6 in intellectual disability and UBR4 in early dementia). If the novel candidate disease genes in this cohort are independently confirmed, the yield of WES will have increased to 83%, which suggests that most "negative" clinical exome tests are unsolved due to interpretation rather than technical limitations.
MeSH term(s)	Consanguinity ; Exome ; Female ; Genetic Diseases, Inborn/diagnosis ; Genetic Diseases, Inborn/epidemiology ; Genetic Testing ; Genome, Human ; High-Throughput Nucleotide Sequencing ; Homozygote ; Humans ; Male ; Molecular Sequence Annotation ; Morbidity ; Mutation ; Phenotype ; Reproducibility of Results ; Saudi Arabia/epidemiology ; Sequence Analysis, DNA
Language	English
Publishing date	2017-06-09
Publishing country	Germany
Document type	Journal Article
ZDB-ID	223009-4
ISSN	1432-1203 ; 0340-6717
ISSN (online)	1432-1203
ISSN	0340-6717
DOI	10.1007/s00439-017-1821-8
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