LIVIVO - Search results -

Search results

Result 1 - 10 of total 71

Search options

Article ; Online: Thrombin-Binding Aptamer with Inversion of Polarity Sites (IPS): Effect on DNAzyme Activity and Anticoagulant Properties.

Kosman, Joanna / Juskowiak, Bernard

International journal of molecular sciences

2021 Volume 22, Issue 15

Abstract: In this work we examined the properties of thrombin-binding aptamer (TBA) modified by the introduction of inversion of polarity sites (IPS) in order to assess the effect of modification on the activation of TBA to serve as DNAzyme with peroxidase-like ... ...

Abstract	In this work we examined the properties of thrombin-binding aptamer (TBA) modified by the introduction of inversion of polarity sites (IPS) in order to assess the effect of modification on the activation of TBA to serve as DNAzyme with peroxidase-like activity. Two oligonucleotides were designed to possess one (IPS1) or three (IPS2) inversion sites. TBA typically forms antiparallel G-quadruplexes with two G-tetrads, which exhibits very low DNAzyme peroxidise activity. DNAzyme activity is generally attributed to parallel G-quadruplexes. Hence, inversion of polarity was introduced in the TBA molecule to force the change of G-quadruplex topology. All oligonucleotides were characterized using circular dichroism and UV-Vis melting profiles. Next, the activity of the DNAzymes formed by studied oligonucleotides and hemin was investigated. The enhancement of peroxidase activity was observed when inversion of polarity was introduced. DNAzyme based on IPS2 showed the highest peroxidase activity in the presence of K
MeSH term(s)	Anticoagulants/pharmacology ; Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/pharmacology ; Circular Dichroism ; DNA, Catalytic/metabolism ; Fibrinogen/metabolism ; G-Quadruplexes ; Hemin/metabolism ; Humans ; Models, Molecular
Chemical Substances	Anticoagulants ; Aptamers, Nucleotide ; DNA, Catalytic ; thrombin aptamer (145563-68-4) ; Hemin (743LRP9S7N) ; Fibrinogen (9001-32-5)
Language	English
Publishing date	2021-07-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22157902
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Thrombin-Binding Aptamer with Inversion of Polarity Sites (IPS)

Joanna Kosman / Bernard Juskowiak

International Journal of Molecular Sciences, Vol 22, Iss 7902, p

Effect on DNAzyme Activity and Anticoagulant Properties

2021 Volume 7902

Abstract	In this work we examined the properties of thrombin-binding aptamer (TBA) modified by the introduction of inversion of polarity sites (IPS) in order to assess the effect of modification on the activation of TBA to serve as DNAzyme with peroxidase-like activity. Two oligonucleotides were designed to possess one (IPS1) or three (IPS2) inversion sites. TBA typically forms antiparallel G-quadruplexes with two G-tetrads, which exhibits very low DNAzyme peroxidise activity. DNAzyme activity is generally attributed to parallel G-quadruplexes. Hence, inversion of polarity was introduced in the TBA molecule to force the change of G-quadruplex topology. All oligonucleotides were characterized using circular dichroism and UV-Vis melting profiles. Next, the activity of the DNAzymes formed by studied oligonucleotides and hemin was investigated. The enhancement of peroxidase activity was observed when inversion of polarity was introduced. DNAzyme based on IPS2 showed the highest peroxidase activity in the presence of K + or NH 4 + ions. This proves that inversion of polarity can be used to convert a low-activity DNAzyme into a DNAzyme with high activity. Since TBA is known for its anticoagulant properties, the relevant experiments with IPS1 and IPS2 oligonucleotides were performed. Both IPS1 and IPS2 retain some anticoagulant activity in comparison to TBA in the reaction with fibrinogen. Additionally, the introduction of inversion of polarity makes these oligonucleotides more resistant to nucleases.
Keywords	G-quadruplex ; DNAzyme ; inversion of polarity ; thrombin ; TBA ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	540 ; 500
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Conformational rearrangements of G-quadruplex topology promoted by Cu(II) 12-MC

Rajczak, Ewa / Juskowiak, Bernard

International journal of biological macromolecules

2019 Volume 156, Page(s) 1258–1269

Abstract: Cu(II) 12- ... ...

Abstract	Cu(II) 12-MC
MeSH term(s)	Copper/chemistry ; G-Quadruplexes/drug effects ; Lanthanoid Series Elements/chemistry ; Models, Molecular ; Nucleic Acid Denaturation/drug effects ; Organometallic Compounds/chemistry ; Organometallic Compounds/pharmacology ; Telomere/chemistry ; Transition Temperature
Chemical Substances	Lanthanoid Series Elements ; Organometallic Compounds ; Copper (789U1901C5)
Language	English
Publishing date	2019-11-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2019.11.163
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Practical Microwave Synthesis of Carbazole Aldehydes for the Development of DNA-Binding Ligands.

Głuszyńska, Agata / Juskowiak, Bernard

Molecules (Basel, Switzerland)

2019 Volume 24, Issue 5

Abstract: Microwave formylation of carbazole derivatives was investigated and 3-monoaldehydes were obtained in high yield. A potential DNA-binding ligand, 3-[(3-ethyl)-2-vinylbenzothiazolium]-9- ...

Abstract	Microwave formylation of carbazole derivatives was investigated and 3-monoaldehydes were obtained in high yield. A potential DNA-binding ligand, 3-[(3-ethyl)-2-vinylbenzothiazolium]-9-
MeSH term(s)	Aldehydes/chemical synthesis ; Aldehydes/chemistry ; DNA/chemistry ; G-Quadruplexes ; Microwaves
Chemical Substances	Aldehydes ; DNA (9007-49-2)
Language	English
Publishing date	2019-03-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules24050965
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.MO 81: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Sequence Effect on the Activity of DNAzyme with Covalently Attached Hemin and Their Potential Bioanalytical Application.

Kosman, Joanna / Żukowski, Krzysztof / Csáki, Andrea / Fritzsche, Wolfgang / Juskowiak, Bernard

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 2

Abstract: In this work we investigated the effect of a DNA oligonucleotide sequence on the activity of a DNAzyme with covalently attached hemin. For this purpose, we synthesized seven DNA-hemin conjugates. All DNA-hemin conjugates as well as DNA/hemin complexes ... ...

Abstract	In this work we investigated the effect of a DNA oligonucleotide sequence on the activity of a DNAzyme with covalently attached hemin. For this purpose, we synthesized seven DNA-hemin conjugates. All DNA-hemin conjugates as well as DNA/hemin complexes were characterized using circular dichroism, determination of melting temperatures and pKa of hemin. We observed that hemin conjugation in most cases led to the formation of parallel G-quadruplexes in the presence of potassium and increased thermal stability of all studied systems. Although the activity of DNA-hemin conjugates depended on the sequence used, the highest activity was observed for the DNA-hemin conjugate based on a human telomeric sequence. We used this DNAzyme for development of "sandwich" assay for detection of DNA sequence. For this assay, we used electric chip which could conduct electricity after silver deposition catalyzed by DNAzyme. This method was proved to be selective towards DNA oligonucleotides with mismatches and could be used for the detection of the target. To prove the versatility of our DNAzyme probe we also performed experiments with streptavidin-coated microplates. Our research proved that DNAzyme with covalently attached hemin can be used successfully in the development of heterogeneous assays.
MeSH term(s)	Biosensing Techniques ; DNA ; DNA, Catalytic/metabolism ; G-Quadruplexes ; Hemin ; Humans ; Silver
Chemical Substances	DNA, Catalytic ; Silver (3M4G523W1G) ; Hemin (743LRP9S7N) ; DNA (9007-49-2)
Language	English
Publishing date	2022-01-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22020500
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots.

Żukowski, Krzysztof / Kosman, Joanna / Juskowiak, Bernard

International journal of molecular sciences

2020 Volume 21, Issue 21

Abstract: Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an ... ...

Abstract	Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H
MeSH term(s)	Benzothiazoles/chemistry ; Benzothiazoles/metabolism ; DNA, Catalytic/chemistry ; DNA, Catalytic/metabolism ; Light ; Microscopy, Electron, Transmission ; Nanoparticles/chemistry ; Nanoparticles/ultrastructure ; Oxazines/chemistry ; Oxazines/metabolism ; Oxidoreductases/metabolism ; Quantum Dots ; Reactive Oxygen Species/metabolism ; Reactive Oxygen Species/radiation effects ; Spectrum Analysis/methods ; Sulfonic Acids/chemistry ; Sulfonic Acids/metabolism
Chemical Substances	Benzothiazoles ; DNA, Catalytic ; Oxazines ; Reactive Oxygen Species ; Sulfonic Acids ; Amplex Red (119171-73-2) ; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (28752-68-3) ; Oxidoreductases (EC 1.-)
Language	English
Publishing date	2020-11-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21218190
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Monitoring of pH Using an i-Motif-Forming Sequence Containing a Fluorescent Cytosine Analogue, tC.

Bielecka, Patrycja / Dembska, Anna / Juskowiak, Bernard

Molecules (Basel, Switzerland)

2019 Volume 24, Issue 5

Abstract: The i-motif is a four-stranded DNA structure formed from the cytosine (C)-rich ssDNA sequence, which is stabilized in slightly acidic pH. Additionally, labeling of a cytosine-rich sequence with a fluorescent molecule may constitute a way to construct a ... ...

Abstract	The i-motif is a four-stranded DNA structure formed from the cytosine (C)-rich ssDNA sequence, which is stabilized in slightly acidic pH. Additionally, labeling of a cytosine-rich sequence with a fluorescent molecule may constitute a way to construct a pH-sensitive biosensor. In this paper, we report tC-modified fluorescent probes that contain
MeSH term(s)	Cytosine/chemistry ; DNA/chemistry ; Fluorescent Dyes/chemistry ; Hydrogen-Ion Concentration ; Nucleic Acid Conformation
Chemical Substances	Fluorescent Dyes ; Cytosine (8J337D1HZY) ; DNA (9007-49-2)
Language	English
Publishing date	2019-03-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules24050952
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.MO 81: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Nucleic acid-based fluorescent probes and their analytical potential.

Juskowiak, Bernard

Analytical and bioanalytical chemistry

2010 Volume 399, Issue 9, Page(s) 3157–3176

Abstract: It is well known that nucleic acids play an essential role in living organisms because they store and transmit genetic information and use that information to direct the synthesis of proteins. However, less is known about the ability of nucleic acids to ... ...

Abstract	It is well known that nucleic acids play an essential role in living organisms because they store and transmit genetic information and use that information to direct the synthesis of proteins. However, less is known about the ability of nucleic acids to bind specific ligands and the application of oligonucleotides as molecular probes or biosensors. Oligonucleotide probes are single-stranded nucleic acid fragments that can be tailored to have high specificity and affinity for different targets including nucleic acids, proteins, small molecules, and ions. One can divide oligonucleotide-based probes into two main categories: hybridization probes that are based on the formation of complementary base-pairs, and aptamer probes that exploit selective recognition of nonnucleic acid analytes and may be compared with immunosensors. Design and construction of hybridization and aptamer probes are similar. Typically, oligonucleotide (DNA, RNA) with predefined base sequence and length is modified by covalent attachment of reporter groups (one or more fluorophores in fluorescence-based probes). The fluorescent labels act as transducers that transform biorecognition (hybridization, ligand binding) into a fluorescence signal. Fluorescent labels have several advantages, for example high sensitivity and multiple transduction approaches (fluorescence quenching or enhancement, fluorescence anisotropy, fluorescence lifetime, fluorescence resonance energy transfer (FRET), and excimer-monomer light switching). These multiple signaling options combined with the design flexibility of the recognition element (DNA, RNA, PNA, LNA) and various labeling strategies contribute to development of numerous selective and sensitive bioassays. This review covers fundamentals of the design and engineering of oligonucleotide probes, describes typical construction approaches, and discusses examples of probes used both in hybridization studies and in aptamer-based assays.
MeSH term(s)	Biosensing Techniques/instrumentation ; Fluorescence Polarization/instrumentation ; Fluorescence Resonance Energy Transfer/instrumentation ; Fluorescent Dyes/chemistry ; Nucleic Acid Hybridization ; Nucleic Acid Probes/chemistry ; Nucleic Acid Probes/genetics
Chemical Substances	Fluorescent Dyes ; Nucleic Acid Probes
Language	English
Publishing date	2010-10-29
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-010-4304-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 69/97: Show issues
Archiv 3: Show issues
Z 9.4: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences.

Dembska, Anna / Świtalska, Angelika / Fedoruk-Wyszomirska, Agnieszka / Juskowiak, Bernard

Scientific reports

2020 Volume 10, Issue 1, Page(s) 11006

Abstract: The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G- ... ...

Abstract	The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane.
MeSH term(s)	Biosensing Techniques ; Cell Membrane/chemistry ; Cytosine/chemistry ; Fluorescent Dyes/chemistry ; G-Quadruplexes ; Guanine/chemistry ; Metal Nanoparticles ; Models, Molecular ; Oligonucleotide Probes/chemistry ; Silver
Chemical Substances	Fluorescent Dyes ; Oligonucleotide Probes ; Silver (3M4G523W1G) ; Guanine (5Z93L87A1R) ; Cytosine (8J337D1HZY)
Language	English
Publishing date	2020-07-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-67745-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Light-Induced Oxidase Activity of DNAzyme-Modified Quantum Dots

Krzysztof Żukowski / Joanna Kosman / Bernard Juskowiak

International Journal of Molecular Sciences, Vol 21, Iss 8190, p

2020 Volume 8190

Abstract: Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H 2 O 2 -free DNAzyme system with oxidase activity. Amino-coupling conjugation was carried out between amino-modified oligonucleotides (CatG4-NH 2 ) and ... ...

Abstract	Here, we report the synthesis of a quantum dot (QD)-DNA covalent conjugate to be used as an H 2 O 2 -free DNAzyme system with oxidase activity. Amino-coupling conjugation was carried out between amino-modified oligonucleotides (CatG4-NH 2 ) and carboxylated quantum dots (CdTe@COOH QDs). The obtained products were characterized by spectroscopic methods (UV-Vis, fluorescence, circular dichroizm (CD), and IR) and the transmission electron microscopy (TEM) technique. A QD-DNA system with a low polydispersity and high stability in aqueous solutions was successfully obtained. The catalytic activity of the QD-DNA conjugate was examined with Amplex Red and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)) indicators using reactive oxygen species (ROS) generated by visible light irradiation. The synthesized QD-DNAzyme exhibited enhanced catalytic activity compared with the reference system (a mixture of QDs and DNAzyme). This proved the assumption that the covalent attachment of DNAzyme to the surface of QD resulted in a beneficial effect on its catalytic activity. The results proved that the QD-DNAzyme system can be used for generation of the signal by light irradiation. The light-induced oxidase activity of the conjugate was demonstrated, proving that the QD-DNAzyme system can be useful for the development of new cellular bioassays, e.g., for the determination of oxygen radical scavengers.
Keywords	quantum dots (QDs) ; DNAzyme ; ROS ; Amplex Red ; light-induced activity ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	540
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED