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Article ; Online: Correction

Lee Shun J / Wang Jean YJ

BMC Biology, Vol 8, Iss 1, p

Exploiting the promiscuity of imatinib

2010 Volume 82

Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2010-06-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Nucleo-cytoplasmic communication in apoptotic response to genotoxic and inflammatory stress.

Wang, Jean Yj

Cell research

2005 Volume 15, Issue 1, Page(s) 43–48

Abstract: Genotoxic agents or inflammatory cytokines activate cellular stress responses and trigger programmed cell death. We have identified a signal transduction module, including three nuclear proteins that participate in the regulation of cell death induced by ...

Abstract	Genotoxic agents or inflammatory cytokines activate cellular stress responses and trigger programmed cell death. We have identified a signal transduction module, including three nuclear proteins that participate in the regulation of cell death induced by chemotherapeutic agents and tumor necrosis factor (TNF). In this nuclear signaling module, retinoblastoma protein (Rb) functions as an inhibitor of apoptotic signal transduction. Inactivation of Rb by phosphorylation or caspase-dependent cleavage/degradation is required for cell death to occur. Rb inhibits the Abl tyrosine kinase. Thus, Rb inactivation is a pre-requisite for Abl activation by DNA damage or TNF. Activation of nuclear Abl and its downstream effector p73 induces mitochondriadependent cell death. The involvement of these nuclear signal transducers in TNF induced apoptosis, which does not require new gene expression, indicates that nuclear events other than transcription can contribute to extrinsic apoptotic signal transduction.
MeSH term(s)	Animals ; Apoptosis ; Cell Death ; Cell Nucleus/metabolism ; Cytokines/metabolism ; Cytoplasm/metabolism ; DNA Damage ; DNA-Binding Proteins/metabolism ; Genes, Tumor Suppressor ; Humans ; Inflammation ; Mice ; Mitochondria/metabolism ; Models, Biological ; Nuclear Proteins/metabolism ; Retinoblastoma Protein/metabolism ; Signal Transduction ; Tumor Necrosis Factor-alpha/metabolism ; Tumor Necrosis Factors ; Tumor Protein p73 ; Tumor Suppressor Protein p53/metabolism ; Tumor Suppressor Proteins
Chemical Substances	Cytokines ; DNA-Binding Proteins ; Nuclear Proteins ; Retinoblastoma Protein ; TP73 protein, human ; Trp73 protein, mouse ; Tumor Necrosis Factor-alpha ; Tumor Necrosis Factors ; Tumor Protein p73 ; Tumor Suppressor Protein p53 ; Tumor Suppressor Proteins
Language	English
Publishing date	2005-01-27
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; Review
ZDB-ID	1319303-x
ISSN	1748-7838 ; 1001-0602
ISSN (online)	1748-7838
ISSN	1001-0602
DOI	10.1038/sj.cr.7290263
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Article ; Online: Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors.

Ludwig, Kirsten / Tse, Edison S / Wang, Jean Yj

BMC cancer

2013 Volume 13, Page(s) 221

Abstract: Background: The intestinal crypt homeostasis is maintained by a combination of growth factors including Wnt, R-Spondin1, Noggin and the epidermal growth factor (EGF). In human colorectal cancer, the Wnt pathway is constitutively activated through ... ...

Abstract	Background: The intestinal crypt homeostasis is maintained by a combination of growth factors including Wnt, R-Spondin1, Noggin and the epidermal growth factor (EGF). In human colorectal cancer, the Wnt pathway is constitutively activated through genetic and epigenetic alterations in as many as 11 genes encoding components of this crypt stem-cell maintenance mechanism. Although the proliferation of colon cancer cells does not require Wnt, it is possible that colon cancer cells can still respond to the crypt growth factors in the colonic microenvironment. A number of studies have shown that epithelial cells behave differently in 3-D versus 2-D cultures. Because the 3-D conditions more closely mimic the in vivo environment, we examined the effects of Wnt and other crypt growth factors on colon cancer cell growth in 3-D culture. Methods: Colon cancer cells were grown in 3-D matrigel supplemented with different combinations of crypt growth factors and colonies were examined for morphology and pathways. Results: When colon cancer cells were cultured in 3-D with EGF, they grew as round spheroid colonies. However, colon cancer cells also grew as flat, disc-like colonies when cultured with EGF plus Wnt, R-Spondin1 and Noggin. Disc colonies were found to have comparable levels of E-cadherin as the spheroid colonies, but showed decreased E-cadherin at the cell-matrix contact sites. Disc colonies also elaborated F-actin rich protrusions (FRP) at the cell-matrix edge, reminiscent of an invasive phenotype but without the expression of vimentin. These E-cadherin and F-actin alterations were not induced by the four growth factors in 2-D culture. Formation of the disc colonies was inhibited by the knockdown of β-catenin and by protein kinase inhibitors such as gefitinib, imatinib and MK-2206. Furthermore, withdrawal of the crypt growth factors was able to revert the disc colonies to spheroid growth, showing that the invasive phenotype was reversible dependent on the availability of growth factors. Conclusions: These findings show that colon cancer cells remain responsive to the growth factors in the crypt microenvironment and can be induced to undergo morphological transformation in the more physiologically relevant 3-D culture.
MeSH term(s)	Blotting, Western ; Cell Culture Techniques/methods ; Cell Proliferation/drug effects ; Colonic Neoplasms/pathology ; Epithelial-Mesenchymal Transition/drug effects ; Fluorescent Antibody Technique ; HCT116 Cells ; HT29 Cells ; Humans ; Intercellular Signaling Peptides and Proteins/pharmacology ; Microscopy, Confocal ; Phenotype ; Signal Transduction/drug effects ; Signal Transduction/physiology
Chemical Substances	Intercellular Signaling Peptides and Proteins
Language	English
Publishing date	2013-05-02
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2041352-X
ISSN	1471-2407 ; 1471-2407
ISSN (online)	1471-2407
ISSN	1471-2407
DOI	10.1186/1471-2407-13-221
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion

Huang XiaoDong / Wang Jean YJ / Lu Xin

BMC Systems Biology, Vol 2, Iss 1, p

2008 Volume 49

Abstract: Abstract Background High throughput screens with RNA interference technology enable loss-of-function analyses of gene activities in mammalian cells. While the construction of genome-scale shRNA libraries has been successful, results of large-scale ... ...

Abstract	Abstract Background High throughput screens with RNA interference technology enable loss-of-function analyses of gene activities in mammalian cells. While the construction of genome-scale shRNA libraries has been successful, results of large-scale screening of those libraries can be difficult to analyze because of the relatively high noise levels and the fact that not all shRNAs in a library are equally effective in silencing gene expression. Results We have screened a library consisting of 43,828 shRNAs directed against 8,500 human genes for functions that are necessary in cell detachment induced by a constitutively activated c-Abl tyrosine kinase. To deal with the issues of noise and uncertainty of knockdown efficiencies, we employed an analytical strategy that combines quantitative data analysis with biological knowledge, i.e. Gene Ontology and pathway information, to increase the power of the RNAi screening technique. Using this strategy we found 16 candidate genes to be involved in Abl-induced disruption of cell adhesion, and verified that the knockdown of IL6ST is associated with enhanced cell attachment. Conclusion Our results suggest that the power of genome-wide quantitative shRNA screens can be significantly increased when analyzed using a systems biology-based approach to identify functional gene networks.
Keywords	Biology (General) ; QH301-705.5
Subject code	612
Language	English
Publishing date	2008-06-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of hydroxyurea on the promoter occupancy profiles of tumor suppressor p53 and p73

Lu Xin / Huang Vera / Jiang Yong / Wang Jean YJ

BMC Biology, Vol 7, Iss 1, p

2009 Volume 35

Abstract: Abstract Background The p53 tumor suppressor and its related protein, p73, share a homologous DNA binding domain, and mouse genetics studies have suggested that they have overlapping as well as distinct biological functions. Both p53 and p73 are ... ...

Abstract	Abstract Background The p53 tumor suppressor and its related protein, p73, share a homologous DNA binding domain, and mouse genetics studies have suggested that they have overlapping as well as distinct biological functions. Both p53 and p73 are activated by genotoxic stress to regulate an array of cellular responses. Previous studies have suggested that p53 and p73 independently activate the cellular apoptotic program in response to cytotoxic drugs. The goal of this study was to compare the promoter-binding activity of p53 and p73 at steady state and after genotoxic stress induced by hydroxyurea. Results We employed chromatin immunoprecipitation, the NimbleGen promoter arrays and a model-based algorithm for promoter arrays to identify promoter sequences enriched in anti-p53 or anti-p73 immunoprecipitates, either before or after treatment with hydroxyurea, which increased the expression of both p53 and p73 in the human colon cancer cell line HCT116-3(6). We calculated a model-based algorithm for promoter array score for each promoter and found a significant correlation between the promoter occupancy profiles of p53 and p73. We also found that after hydroxyurea treatment, the p53-bound promoters were still bound by p73, but p73 became associated with additional promoters that that did not bind p53. In particular, we showed that hydroxyurea induces the binding of p73 but not p53 to the promoter of MLH3 , which encodes a mismatch repair protein, and causes an up-regulation of the MLH3 mRNA. Conclusion These results suggest that hydroxyurea exerts differential effects on the promoter-binding functions of p53 and p73 and illustrate the power of model-based algorithm for promoter array in the analyses of promoter occupancy profiles of highly homologous transcription factors.
Keywords	Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2009-06-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Development of vessel mimicking microfluidic device for studying mechano-response of endothelial cells.

Chu, Pei-Yu / Hsieh, Han-Yun / Chung, Pei-Shan / Wang, Pai-Wen / Wu, Ming-Chung / Chen, Yin-Quan / Kuo, Jean-Cheng / Fan, Yu-Jui

iScience

2023 Volume 26, Issue 6, Page(s) 106927

Abstract: The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. ...

Abstract	The objective of this study is to develop a device to mimic a microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS), which are resulting from blood flow and blood pressure, respectively. The device can reveal real-time observation of dynamic morphological change of cells in different flow fields (continuous flow, reciprocating flow and pulsatile flow) and stretch. We observe the effects of FSS and CS on endothelial cells (ECs), including ECs align their cytoskeleton proteins with the fluid flow direction and paxillin redistribution to the cell periphery or the end of stress fibers. Thus, understanding the morphological and functional changes of endothelial cells on physical stimuli can help us to prevent and improve the treatment of cardiovascular diseases.
Language	English
Publishing date	2023-05-19
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2023.106927
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Article ; Online: Inhibitory Perturbations of Fluvastatin on Afterhyperpolarization Current, Erg-mediated K

Wang, Ya-Jean / Yeh, Che-Jui / Gao, Zi-Han / Hwang, Eric / Chen, Hwei-Hisen / Wu, Sheng-Nan

Neuroscience

2023 Volume 531, Page(s) 12–23

Abstract: Fluvastatin (FLV), the first synthetically derived 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is a potent inhibitor of cholesterol biosynthesis. While its primary mechanism of action is to reduce cholesterol levels, there is some evidence ...

Abstract	Fluvastatin (FLV), the first synthetically derived 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is a potent inhibitor of cholesterol biosynthesis. While its primary mechanism of action is to reduce cholesterol levels, there is some evidence suggesting that it may also have effects on K
MeSH term(s)	Mice ; Animals ; Fluvastatin ; Pituitary Gland ; Neurons/physiology ; Cations ; Cholesterol
Chemical Substances	Fluvastatin (4L066368AS) ; Cations ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2023-09-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	196739-3
ISSN	1873-7544 ; 0306-4522
ISSN (online)	1873-7544
ISSN	0306-4522
DOI	10.1016/j.neuroscience.2023.08.038
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Article ; Online: Hydride Doping Effects on the Structure and Properties of Eight-Electron Rh/Ag Superatoms: The [RhH

Chiu, Tzu-Hao / Liao, Jian-Hong / Wu, Ying-Yann / Chen, Jie-Ying / Chen, Yuan Jang / Wang, Xiaoping / Kahlal, Samia / Saillard, Jean-Yves / Liu, C W

Journal of the American Chemical Society

2023 Volume 145, Issue 30, Page(s) 16739–16747

Abstract: Three hitherto unknown eight-electron rhodium/silver alloy nanoclusters, [ ... ...

Abstract	Three hitherto unknown eight-electron rhodium/silver alloy nanoclusters, [RhAg
Language	English
Publishing date	2023-07-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.3c04482
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Article ; Online: Author Correction: Higher Delta variant-specific neutralizing antibodies prevented infection in close contacts vaccinated with ancestral mRNA vaccines during the SARS-CoV-2 Delta wave.

Goh, Yun Shan / Fong, Siew-Wai / Tay, Matthew Zirui / Rouers, Angeline / Chang, Zi Wei / Chavatte, Jean-Marc / Hor, Pei Xiang / Loh, Chiew Yee / Huang, Yuling / Tan, Yong Jie / Wang, Bei / Ngoh, Eve Zi Xian / Mohd Salleh, Siti Nazihah / Lee, Raphael Tze Chuen / Lim, Georgina / Maurer-Stroh, Sebastian / Wang, Cheng-I / Leo, Yee-Sin / Lin, Raymond T P /

Lam, Meng Chon / Lye, David C / Young, Barnaby Edward / Ng, Lisa F P / Renia, Laurent

Scientific reports

2024 Volume 14, Issue 1, Page(s) 1492

Language	English
Publishing date	2024-01-17
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-51484-y
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Article ; Online: The complete mitochondrial genome sequence of Belligobio nummifer (Cypriniformes, Cyprinidae).

Wang, Feng-Yu / Jean, Chuen-Tan / Chen, Yi-Jie / Lin, Kuan-Yu / Liu, Min-Yun

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis

2016 Volume 27, Issue 1, Page(s) 435–436

Abstract: We determined the complete mitochondrial genome (mitogenome) sequence of Belligobio nummifer, which is known as a cyprinid fish in mainland China, with a long polymerase chain reaction (PCR) method. The total length of B. nummifer mitogenome is 16,610 bp, ...

Abstract	We determined the complete mitochondrial genome (mitogenome) sequence of Belligobio nummifer, which is known as a cyprinid fish in mainland China, with a long polymerase chain reaction (PCR) method. The total length of B. nummifer mitogenome is 16,610 bp, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region. The overall base composition of B. nummifer is 29.74% for A, 26.12% for T, 17.18% for G, and 26.97% for C, with a slight AT bias of 55.86%. The complete mitogenomic data may provide more informative for phylogenetic approach for gudgeons phylogeny.
MeSH term(s)	Animals ; Cyprinidae/genetics ; DNA, Mitochondrial/genetics ; Genome, Mitochondrial/genetics ; Molecular Sequence Annotation ; Molecular Sequence Data ; Sequence Analysis, DNA
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2016
Publishing country	England
Document type	Journal Article
ISSN	2470-1408
ISSN (online)	2470-1408
DOI	10.3109/19401736.2014.898293
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