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Book ; Online ; Thesis: Prävalenz von MRSA und ESBL-bildenden E. coli bei landwirtschaftlichen Mitarbeitern und Nutztieren in Mecklenburg-Vorpommern

Dahms, Carmen [Verfasser]

2017

Author's details	Carmen Dahms
Keywords	Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
Subject code	sg630
Language	German
Publisher	Universitätsbibliothek Greifswald
Publishing place	Greifswald
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Book ; Online: Prävalenz von MRSA und ESBL-bildenden E. coli bei landwirtschaftlichen Mitarbeitern und Nutztieren in Mecklenburg-Vorpommern

Dahms, Carmen

2016

Abstract: MRSA, Extended-Spectrum Beta-Lactamase, Zoonose, Nutztiere, Multidrug-Resistenz ... Multiresistant bacteria are known as human pathogens causing nosocomial infections, but they are also harmful in veterinary medicine. Livestock are often asymptomatic ... ...

Institution	Ernst-Moritz-Arndt-Universität Greifswald
Author's details	vorgelegt von: Carmen Dahms
Abstract	MRSA, Extended-Spectrum Beta-Lactamase, Zoonose, Nutztiere, Multidrug-Resistenz Multiresistant bacteria are known as human pathogens causing nosocomial infections, but they are also harmful in veterinary medicine. Livestock are often asymptomatic carriers and therefore an important reservoir. This study was meant to present data on the occurrence of methicillin-resistant S. aureus (MRSA) and extended-spectrum β-lactamases (ESBL) producing E. coli in farm workers and livestock in Mecklenburg Western-Pomerania in 2012. In total, 17 pig farms, 11 cattle farms, 6 poultry farms and 78 farm workers were included. For human MRSA sampling combined nasopharyngeal swabs were taken. To find out the animal colonization rate, pooled dust samples were collected. Additionally, at each poultry farm oropharyngeal swabs of randomly chosen animals were taken. Antimicrobial susceptibility testing was performed using the broth microdilution assay. Furthermore, spa-typing, multilocus sequence typing (MLST) and polymerase chain reaction (PCR) identifying the gene luk-PV was performed. In total, 20 of 78 pig farm workers tested positive for MRSA. Six of 34 pooled dust samples were MRSA-positive. All MRSA isolates belonged to the clonal complex (CC) 398 and were therefore livestock-associated MRSA (LA-MRSA). None of the isolates contained the gene luk-PV which encodes the toxin Panton-Valentine-leukocidin. The spa-types t034 (9/26), t011 (7/26) and t2370 (7/26) predominated. All isolates were resistant to oxacillin and tetracycline, the most common resistance phenotype ...
Language	German
Size	1 Online-Ressource (PDF-Datei: 154 Seiten, 1862 Kilobyte), Illustrationen (farbig), Diagramme (farbig)
Publishing place	Greifswald
Document type	Book ; Online
Note	Literaturverzeichnis: Seite 108-127
Database	Special collection on veterinary medicine and general parasitology

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Article: Mini-review: Epidemiology and zoonotic potential of multiresistant bacteria and Clostridium difficile in livestock and food.

Dahms, Carmen / Hübner, Nils-Olaf / Wilke, Florian / Kramer, Axel

GMS hygiene and infection control

2014 Volume 9, Issue 3, Page(s) Doc21

Abstract: Aim: Information on the epidemiology of multiresistant bacteria (MRB) with zoonotic potential is growing but still remains quite incomplete. This narrative mini-review provides a general overview of the epidemiology of the most important zoonotic MRB in ...

Abstract	Aim: Information on the epidemiology of multiresistant bacteria (MRB) with zoonotic potential is growing but still remains quite incomplete. This narrative mini-review provides a general overview of the epidemiology of the most important zoonotic MRB in cattle, swine and poultry in Europe. Methods: A literature search was conducted mainly on the PubMed website including articles published until April 2012. Results: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) especially poses a zoonotic risk to people working in close contact with livestock. These people may become carriers themselves and the hazard of transmission into health-care facilities needs surveillance. Extended-spectrum beta-lactamases (ESBL) producing bacteria are widely spread in both humans and livestock, sharing similar genotypes, especially of the CTX-M-group, which makes a zoonotic transfer very likely. Identical strains of vancomycin-resistant enterococci (VRE) were found both in humans and animals, after ingestion of animal strains transient colonization of the human gut may be possible. Only a few data are available on the transmission of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) between humans and animals. Direct contact to colonized animals may be a risk factor as well as the exchange of resistance genes between human and animal staphylococci. Clostridium difficile (C. difficile) ribotype 078 emerges in livestock and humans and a zoonotic transmission seems probable as genotypes and diseases resemble each other. Conclusion: All discussed MRB and C. difficile are important nosocomial agents which also occur in livestock and were found in foods of animal origin. Further analysis is needed to reveal the exact transmission routes and to perform a reliable risk assessment.
Language	English
Publishing date	2014-09-30
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2717852-3
ISSN	2196-5226
ISSN	2196-5226
DOI	10.3205/dgkh000241
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Occurrence of methicillin-resistant Staphylococcus aureus in farm workers and the livestock environment in Mecklenburg-Western Pomerania, Germany.

Dahms, Carmen / Hübner, Nils-Olaf / Cuny, Christiane / Kramer, Axel

Acta veterinaria Scandinavica

2014 Volume 56, Page(s) 53

Abstract: Background: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has a wide host range and is transmissible to humans, especially to those with close contact to colonized animals. This study presents the first data on the ... ...

Abstract	Background: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has a wide host range and is transmissible to humans, especially to those with close contact to colonized animals. This study presents the first data on the occurrence of MRSA in farm workers and livestock farms (pig, cattle and poultry) in the federal state of Mecklenburg-Western Pomerania in northeast Germany. 78 farm workers at pig farms, cattle farms and poultry farms were tested for MRSA via pooled nasal and pharyngeal swabs. Additionally, from each of the 34 participating farms (17 pig farms, 11 cattle farms, 6 poultry farms) five dust samples were taken from the direct surroundings of the animals. Furthermore, oropharyngeal swabs were additionally taken from 10 animals per poultry farm. Isolated MRSA strains were characterized and confirmed using PCR and spa typing. Resistance patterns were obtained using the broth microdilution assay. Results: In total, 20 of 78 (25.6%; 95% CI:17.3-36.3) farm workers were positive for MRSA. All MRSA-positive workers were employed at pig farms. Six of 17 (35.3%; 95% CI:17.3-58.7) pooled dust samples from pig farms were also positive. Overall, six spa types were identified, of which t034 predominated. All strains belonged to LA-MRSA CC398 and were resistant to tetracycline. Resistance to lincosamides, macrolides, fluoroquinolones and aminoglycosides was present in some strains. Three farm workers harbored the identical spa type and antimicrobial resistance pattern found in the corresponding dust sample. Neither workers, dust samples from cattle and poultry farms, nor oropharyngeal poultry swabs tested positive for MRSA. Conclusions: The present study emphasizes the importance of MRSA on pig farms and pig-farm workers in the rural region of Mecklenburg-Western Pomerania, whereas LA-MRSA could not be isolated from cattle and poultry farms.
MeSH term(s)	Animal Diseases/epidemiology ; Animal Diseases/microbiology ; Animals ; Cattle ; Cross-Sectional Studies ; Dust/analysis ; Environmental Microbiology ; Female ; Germany/epidemiology ; Humans ; Male ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Occupational Diseases/epidemiology ; Occupational Diseases/microbiology ; Poultry ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Swine
Chemical Substances	Dust
Language	English
Publishing date	2014-08-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	40137-7
ISSN	1751-0147 ; 0044-605X
ISSN (online)	1751-0147
ISSN	0044-605X
DOI	10.1186/s13028-014-0053-3
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Zs.B 484: Show issues

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Article ; Online: Development and evaluation of a novel universal β-lactamase gene subtyping assay for blaSHV, blaTEM and blaCTX-M using clinical and livestock-associated Escherichia coli.

Strauß, Lena Marie / Dahms, Carmen / Becker, Karsten / Kramer, Axel / Kaase, Martin / Mellmann, Alexander

The Journal of antimicrobial chemotherapy

2015 Volume 70, Issue 3, Page(s) 710–715

Abstract: Objectives: Antibiotic resistance among Escherichia coli is globally an increasing problem in public healthcare. Understanding the spread of plasmid-mediated ESBL genes is of great importance in elucidating their molecular epidemiology. However, ... ...

Abstract	Objectives: Antibiotic resistance among Escherichia coli is globally an increasing problem in public healthcare. Understanding the spread of plasmid-mediated ESBL genes is of great importance in elucidating their molecular epidemiology. However, differentiation of subtypes and alleles is frequently hampered by the lack of comprehensive diagnostic tools. We therefore developed a novel universal blaSHV, blaTEM and blaCTX-M subtyping assay based on PCR and Sanger sequencing that results in large amplicons of >700 bp, enabling differentiation of bla alleles as precisely as possible. Methods: The assay was established using 10 reference strains with known bla genotypes that represent all examined primer groups and 101 uncharacterized ESBL-producing E. coli of clinical and livestock-associated origins from different German regions. All isolates were tested in parallel with established blaSHV, blaTEM and blaCTX-M subtyping assays for the respective β-lactamases and their alleles. Results: The novel assay yielded equal (n = 92) or better (n = 47) subtyping results compared with established subtyping methods and reliably detected all expected enzymes in the reference strains. Overall, the occurring enzymes could be differentiated into groups representing one (n = 9), two (n = 5) or three (n = 4) highly similar alleles. Moreover, ESBL and non-ESBL allelic variants of blaSHV and blaTEM occurring in the same isolate were distinguished reliably. Conclusions: We established a highly discriminatory assay for the subtyping of clinically important ESBL genes that can easily be used in epidemiological analyses.
MeSH term(s)	Animals ; Escherichia coli/enzymology ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Escherichia coli Infections/microbiology ; Escherichia coli Infections/veterinary ; Genotype ; Genotyping Techniques/methods ; Humans ; Livestock ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; beta-Lactamases/classification ; beta-Lactamases/genetics
Chemical Substances	beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2015-03
Publishing country	England
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dku450
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 124: Show issues

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Article ; Online: Occurrence of ESBL-Producing Escherichia coli in Livestock and Farm Workers in Mecklenburg-Western Pomerania, Germany.

Dahms, Carmen / Hübner, Nils-Olaf / Kossow, Annelene / Mellmann, Alexander / Dittmann, Kathleen / Kramer, Axel

PloS one

2015 Volume 10, Issue 11, Page(s) e0143326

Abstract: In recent years, extended-spectrum β-lactamases (ESBL) producing bacteria have been found in livestock, mainly as asymptomatic colonizers. The zoonotic risk for people working in close contact to animal husbandry has still not been completely assessed. ... ...

Abstract	In recent years, extended-spectrum β-lactamases (ESBL) producing bacteria have been found in livestock, mainly as asymptomatic colonizers. The zoonotic risk for people working in close contact to animal husbandry has still not been completely assessed. Therefore, we investigated the prevalence of ESBL-producing Escherichia spp. in livestock animals and workers to determine the potential risk for an animal-human cross-transmission.In Mecklenburg-Western Pomerania, northeast Germany, inguinal swabs of 73 individuals with livestock contact from 23 different farms were tested for ESBL-producing Escherichia spp. Two pooled fecal samples per farm of animal origin from 34 different farms (17 pig farms, 11 cattle farms, 6 poultry farms) as well as cloacal swabs of 10 randomly selected broilers or turkeys were taken at each poultry farm. For identification, selective chromogenic agar was used after an enrichment step. Phenotypically ESBL-producing isolates (n = 99) were tested for CTX-M, OXA, SHV and TEM using PCR, and isolates were further characterized using multilocus sequence typing (MLST). In total, 61 diverse isolates from different sources and/or different MLST/PCR results were acquired. Five farm workers (three from cattle farms and two from pig farms) harbored ESBL-producing E. coli. All human isolates harbored the CTX-M β-lactamase; TEM and OXA β-lactamases were additionally detected in two, resp. one, isolates. ESBL-producing Escherichia spp. were found in fecal samples at pig (15/17), cattle (6/11) and poultry farms (3/6). In total, 70.6% (24/36) of the tested farms were ESBL positive. Furthermore, 9 out of 60 cloacal swabs turned out to be ESBL positive. All isolated ESBL-producing bacteria from animal sources were E. coli, except for one E. hermanii isolate. CTX-M was the most prevalent β-lactamase at cattle and pig farms, while SHV predominated in poultry. One human isolate shared an identical MLST sequence type (ST) 3891 and CTX-M allele to the isolate found in the cattle fecal sample from the same farm, indicating a zoonotic transfer. Two other pairs of human-pig and human-cattle E. coli isolates encoded the same ESBL genes but did not share the same MLST ST, which may indicate horizontal resistance gene transfer. In summary, the study shows the high prevalence of ESBL-producing E.coli in livestock in Mecklenburg- Western Pomerania and provides the risk of transfer between livestock and farm workers.
MeSH term(s)	Animal Diseases/epidemiology ; Animal Diseases/microbiology ; Animals ; Cattle ; Cross-Sectional Studies ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Escherichia coli/metabolism ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Farmers ; Feces/microbiology ; Female ; Germany/epidemiology ; Humans ; Livestock/microbiology ; Male ; Multilocus Sequence Typing ; Phylogeny ; Poultry ; Swine ; Turkeys ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
Chemical Substances	beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0143326
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Occurrence of ESBL-Producing Escherichia coli in Livestock and Farm Workers in Mecklenburg-Western Pomerania, Germany.

Carmen Dahms / Nils-Olaf Hübner / Annelene Kossow / Alexander Mellmann / Kathleen Dittmann / Axel Kramer

PLoS ONE, Vol 10, Iss 11, p e

2015 Volume 0143326

Abstract	In recent years, extended-spectrum β-lactamases (ESBL) producing bacteria have been found in livestock, mainly as asymptomatic colonizers. The zoonotic risk for people working in close contact to animal husbandry has still not been completely assessed. Therefore, we investigated the prevalence of ESBL-producing Escherichia spp. in livestock animals and workers to determine the potential risk for an animal-human cross-transmission.In Mecklenburg-Western Pomerania, northeast Germany, inguinal swabs of 73 individuals with livestock contact from 23 different farms were tested for ESBL-producing Escherichia spp. Two pooled fecal samples per farm of animal origin from 34 different farms (17 pig farms, 11 cattle farms, 6 poultry farms) as well as cloacal swabs of 10 randomly selected broilers or turkeys were taken at each poultry farm. For identification, selective chromogenic agar was used after an enrichment step. Phenotypically ESBL-producing isolates (n = 99) were tested for CTX-M, OXA, SHV and TEM using PCR, and isolates were further characterized using multilocus sequence typing (MLST). In total, 61 diverse isolates from different sources and/or different MLST/PCR results were acquired. Five farm workers (three from cattle farms and two from pig farms) harbored ESBL-producing E. coli. All human isolates harbored the CTX-M β-lactamase; TEM and OXA β-lactamases were additionally detected in two, resp. one, isolates. ESBL-producing Escherichia spp. were found in fecal samples at pig (15/17), cattle (6/11) and poultry farms (3/6). In total, 70.6% (24/36) of the tested farms were ESBL positive. Furthermore, 9 out of 60 cloacal swabs turned out to be ESBL positive. All isolated ESBL-producing bacteria from animal sources were E. coli, except for one E. hermanii isolate. CTX-M was the most prevalent β-lactamase at cattle and pig farms, while SHV predominated in poultry. One human isolate shared an identical MLST sequence type (ST) 3891 and CTX-M allele to the isolate found in the cattle fecal sample from the same farm, indicating a zoonotic transfer. Two other pairs of human-pig and human-cattle E. coli isolates encoded the same ESBL genes but did not share the same MLST ST, which may indicate horizontal resistance gene transfer. In summary, the study shows the high prevalence of ESBL-producing E.coli in livestock in Mecklenburg- Western Pomerania and provides the risk of transfer between livestock and farm workers.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Mini-review

Dahms, Carmen / Hübner, Nils-Olaf / Wilke, Florian / Kramer, Axel

GMS Hygiene and Infection Control, Vol 9, Iss 3, p Doc

Epidemiology and zoonotic potential of multiresistant bacteria and Clostridium difficile in livestock and food

2014 Volume 21

Abstract: english] Aim: Information on the epidemiology of multiresistant bacteria (MRB) with zoonotic potential is growing but still remains quite incomplete. This narrative mini-review provides a general overview of the epidemiology of the most important ... ...

Abstract	[english] Aim: Information on the epidemiology of multiresistant bacteria (MRB) with zoonotic potential is growing but still remains quite incomplete. This narrative mini-review provides a general overview of the epidemiology of the most important zoonotic MRB in cattle, swine and poultry in Europe.Methods: A literature search was conducted mainly on the PubMed website including articles published until April 2012. Results: Livestock-associated methicillin-resistant (LA-MRSA) especially poses a zoonotic risk to people working in close contact with livestock. These people may become carriers themselves and the hazard of transmission into health-care facilities needs surveillance. Extended-spectrum beta-lactamases (ESBL) producing bacteria are widely spread in both humans and livestock, sharing similar genotypes, especially of the CTX-M-group, which makes a zoonotic transfer very likely. Identical strains of vancomycin-resistant enterococci (VRE) were found both in humans and animals, after ingestion of animal strains transient colonization of the human gut may be possible. Only a few data are available on the transmission of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) between humans and animals. Direct contact to colonized animals may be a risk factor as well as the exchange of resistance genes between human and animal staphylococci. () ribotype 078 emerges in livestock and humans and a zoonotic transmission seems probable as genotypes and diseases resemble each other.Conclusion: All discussed MRB and are important nosocomial agents which also occur in livestock and were found in foods of animal origin. Further analysis is needed to reveal the exact transmission routes and to perform a reliable risk assessment.
Keywords	livestock ; zoonoses ; MRSA ; Clostridium difficile ; E. coli ; ESBL ; vancomycin ; VRE ; multiresistant ; Medicine ; R ; Public aspects of medicine ; RA1-1270 ; Microbiology ; QR1-502
Subject code	630
Language	German
Publishing date	2014-09-01T00:00:00Z
Publisher	German Medical Science GMS Publishing House
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Occurrence of methicillin-resistant Staphylococcus aureus in farm workers and the livestock environment in Mecklenburg-Western Pomerania, Germany

Dahms, Carmen / Hübner, Nils-Olaf / Cuny, Christiane / Kramer, Axel

2014

Abstract	Background: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has a wide host range and is transmissible to humans, especially to those with close contact to colonized animals. This study presents the first data on the occurrence of MRSA in farm workers and livestock farms (pig, cattle and poultry) in the federal state of Mecklenburg-Western Pomerania in northeast Germany. 78 farm workers at pig farms, cattle farms and poultry farms were tested for MRSA via pooled nasal and pharyngeal swabs. Additionally, from each of the 34 participating farms (17 pig farms, 11 cattle farms, 6 poultry farms) five dust samples were taken from the direct surroundings of the animals. Furthermore, oropharyngeal swabs were additionally taken from 10 animals per poultry farm. Isolated MRSA strains were characterized and confirmed using PCR and spa typing. Resistance patterns were obtained using the broth microdilution assay. Results: In total, 20 of 78 (25.6%; 95% CI:17.3-36.3) farm workers were positive for MRSA. All MRSA-positive workers were employed at pig farms. Six of 17 (35.3%; 95% CI:17.3-58.7) pooled dust samples from pig farms were also positive. Overall, six spa types were identified, of which t034 predominated. All strains belonged to LA-MRSA CC398 and were resistant to tetracycline. Resistance to lincosamides, macrolides, fluoroquinolones and aminoglycosides was present in some strains. Three farm workers harbored the identical spa type and antimicrobial resistance pattern found in the corresponding dust sample. Neither workers, dust samples from cattle and poultry farms, nor oropharyngeal poultry swabs tested positive for MRSA. Conclusions: The present study emphasizes the importance of MRSA on pig farms and pig-farm workers in the rural region of Mecklenburg-Western Pomerania, whereas LA-MRSA could not be isolated from cattle and poultry farms.
Keywords	Medizin ; Humans ; Germany ; Animals ; Cattle ; Chickens ; Zoonoses ; Livestock ; MRSA ; Methicillin-resistant Staphylococcus aureus ; Poultry ; Pig ; ddc:610
Subject code	630
Language	English
Publishing date	2014-08-21
Publisher	Infektionskrankheiten / Erreger
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Occurrence of methicillin-resistant Staphylococcus aureus in farm workers and the livestock environment in Mecklenburg-Western Pomerania, Germany

Dahms, Carmen / Hübner, Nils-Olaf / Cuny, Christiane / Kramer, Axel

2014

Abstract	Background: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has a wide host range and is transmissible to humans, especially to those with close contact to colonized animals. This study presents the first data on the occurrence of MRSA in farm workers and livestock farms (pig, cattle and poultry) in the federal state of Mecklenburg-Western Pomerania in northeast Germany. 78 farm workers at pig farms, cattle farms and poultry farms were tested for MRSA via pooled nasal and pharyngeal swabs. Additionally, from each of the 34 participating farms (17 pig farms, 11 cattle farms, 6 poultry farms) five dust samples were taken from the direct surroundings of the animals. Furthermore, oropharyngeal swabs were additionally taken from 10 animals per poultry farm. Isolated MRSA strains were characterized and confirmed using PCR and spa typing. Resistance patterns were obtained using the broth microdilution assay. Results: In total, 20 of 78 (25.6%; 95% CI:17.3-36.3) farm workers were positive for MRSA. All MRSA-positive workers were employed at pig farms. Six of 17 (35.3%; 95% CI:17.3-58.7) pooled dust samples from pig farms were also positive. Overall, six spa types were identified, of which t034 predominated. All strains belonged to LA-MRSA CC398 and were resistant to tetracycline. Resistance to lincosamides, macrolides, fluoroquinolones and aminoglycosides was present in some strains. Three farm workers harbored the identical spa type and antimicrobial resistance pattern found in the corresponding dust sample. Neither workers, dust samples from cattle and poultry farms, nor oropharyngeal poultry swabs tested positive for MRSA. Conclusions: The present study emphasizes the importance of MRSA on pig farms and pig-farm workers in the rural region of Mecklenburg-Western Pomerania, whereas LA-MRSA could not be isolated from cattle and poultry farms.
Keywords	Humans ; Germany ; Animals ; Cattle ; Chickens ; Zoonoses ; Livestock ; MRSA ; Methicillin-resistant Staphylococcus aureus ; Poultry ; Pig ; 610 Medizin ; ddc:610
Subject code	630
Language	English
Publishing date	2014-08-21
Publisher	Robert Koch-Institut, Infektionskrankheiten / Erreger
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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