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  1. Article ; Online: The GCaMP-R Family of Genetically Encoded Ratiometric Calcium Indicators.

    Cho, Jung-Hwa / Swanson, Carter J / Chen, Jeannie / Li, Ang / Lippert, Lisa G / Boye, Shannon E / Rose, Kasey / Sivaramakrishnan, Sivaraj / Chuong, Cheng-Ming / Chow, Robert H

    ACS chemical biology

    2017  Volume 12, Issue 4, Page(s) 1066–1074

    Abstract: We report on GCaMP-Rs, a new family of genetically encoded ratiometric calcium indicators that extend the virtues of the GCaMP proteins to ratiometric measurements. We have engineered a tandem construct of calcium-dependent GCaMP and calcium-independent ... ...

    Abstract We report on GCaMP-Rs, a new family of genetically encoded ratiometric calcium indicators that extend the virtues of the GCaMP proteins to ratiometric measurements. We have engineered a tandem construct of calcium-dependent GCaMP and calcium-independent mCherry fluorescent proteins. The tandem design assures that the two proteins localize in the same cellular compartment(s) and facilitates pixelwise ratiometric measurements; however, Förster resonance energy transfer (FRET) between the fluorophores reduces brightness of the sensor by up to half (depending on the GCaMP variant). To eliminate FRET, we introduced a rigid α-helix, the ER/K helix, between GCaMP and mCherry. Avoiding FRET significantly increases the brightness (notably, even at low calcium concentrations), the signal-to-noise ratio, and the dynamic range.
    MeSH term(s) Animals ; Calcium/metabolism ; Calibration ; Chickens ; Fluorescence Resonance Energy Transfer ; HEK293 Cells ; Humans ; Kinetics ; Luminescent Proteins/metabolism ; Red Fluorescent Protein
    Chemical Substances Luminescent Proteins ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2017-03-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.6b00883
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Helicity of short E-R/K peptides.

    Sommese, Ruth F / Sivaramakrishnan, Sivaraj / Baldwin, Robert L / Spudich, James A

    Protein science : a publication of the Protein Society

    2010  Volume 19, Issue 10, Page(s) 2001–2005

    Abstract: ... interactions, giving rise to E-R/K helices, on the helicity of short peptides was examined using ... circular dichroism. Short E-R/K-based peptides show significant helix content. Peptides containing one or more E-R ... achieved in Arg-based E-R/K peptides eight, six, and five amino acids long. In these short peptides ...

    Abstract Understanding the secondary structure of peptides is important in protein folding, enzyme function, and peptide-based drug design. Previous studies of synthetic Ala-based peptides (>12 a.a.) have demonstrated the role for charged side chain interactions involving Glu/Lys or Glu/Arg spaced three (i, i + 3) or four (i, i + 4) residues apart. The secondary structure of short peptides (<9 a.a.), however, has not been investigated. In this study, the effect of repetitive Glu/Lys or Glu/Arg side chain interactions, giving rise to E-R/K helices, on the helicity of short peptides was examined using circular dichroism. Short E-R/K-based peptides show significant helix content. Peptides containing one or more E-R interactions display greater helicity than those with similar E-K interactions. Significant helicity is achieved in Arg-based E-R/K peptides eight, six, and five amino acids long. In these short peptides, each additional i + 3 and i + 4 salt bridge has substantial contribution to fractional helix content. The E-R/K peptides exhibit a strongly linear melt curve indicative of noncooperative folding. The significant helicity of these short peptides with predictable dependence on number, position, and type of side chain interactions makes them an important consideration in peptide design.
    MeSH term(s) Amino Acid Sequence ; Arginine/chemistry ; Circular Dichroism ; Glutamic Acid/chemistry ; Lysine/chemistry ; Oligopeptides/chemistry ; Protein Structure, Secondary
    Chemical Substances Oligopeptides ; Glutamic Acid (3KX376GY7L) ; Arginine (94ZLA3W45F) ; Lysine (K3Z4F929H6)
    Language English
    Publishing date 2010-07-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1106283-6
    ISSN 1469-896X ; 0961-8368
    ISSN (online) 1469-896X
    ISSN 0961-8368
    DOI 10.1002/pro.469
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    Zs.A 3407: Show issues Location:
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  3. Book ; Online ; E-Book: Diabetic retinopathy

    Sivaraj, Ramesh R. / Dodson, Paul M.

    screening to treatment

    (Oxford diabetes library series ; Oxford medicine online)

    2020  

    Title variant Diabetic retinopathy: screening to treatment
    Author's details Ramesh R. Sivaraj and Paul M. Dodson
    Series title Oxford diabetes library series
    Oxford medicine online
    Keywords Diabetic retinopathy
    Language English
    Size 1 Online-Ressource (xiv, 210 Seiten), Illustrationen
    Edition Second edition
    Publisher Oxford University Press
    Publishing place Oxford
    Publishing country Great Britain
    Document type Book ; Online ; E-Book
    Note Includes index. - Previous edition issued in print: 2008
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020552197
    ISBN 978-0-19-257129-8 ; 9780198834458 ; 0-19-257129-X ; 0198834454
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  4. Article: Identification and characterization of green gram (Vigna radiata (L.) R. wilczek.) photosensitive lines and their significance

    Sunil, N. / Varaprasad, K. S. / Sivaraj, N. / Abraham, Babu / Kumar, Vinod

    Legume research

    2012  Volume 35, Issue 1, Page(s) 78

    Language English
    Document type Article
    ZDB-ID 407856-1
    ISSN 0250-5371
    Database Current Contents Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

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  5. Article: Identification and characterization of green gram [Vigna radiata (L.) r. wilczek.] photosensitive lines and their significance

    Sunil, N. / Varaprasad, K.S. / Sivaraj, N. / Abraham, Babu / Kumar, Vinod

    Legume Research (India)

    (Mar 2012)  Volume v. 35, Issue (1)

    Abstract: One hundred and forty four accessions of green gram germplasm collected from middle Eastern Ghats were characterized and evaluated for 22 qualitative and quantitative traits during post rainy season of 2006_07 and rainy season of 2007. Three accessions ( ... ...

    Abstract One hundred and forty four accessions of green gram germplasm collected from middle Eastern Ghats were characterized and evaluated for 22 qualitative and quantitative traits during post rainy season of 2006_07 and rainy season of 2007. Three accessions (IC546478, IC546486 and IC369703) exhibited primitive characters such as photosensitivity, spreading growth habit and slight twining tendency, which differed significantly in comparison with the released varieties. Accession IC369703 was found promising for the quantitative traits viz. plant height (21.2 cm), primary branches (4.2) and pod clusters per plant (10.2) as against released varieties values ranging from 27_31.6cm, 1.4_2.4, 7.8_9.4 respectively. Based on the present study and previously reported archaeological evidences, it is proposed that green gram could have its origin in the cusp region of inner Western Ghats and inner Eastern Ghats.
    Keywords MUNG BEANS ; VIGNA RADIATA RADIATA ; PHOTOSENSITIVITY ; PROVENANCE ; GERMPLASM ; HARICOT DORE ; VIGNA RADIATA RADIATA ; PHOTOSENSIBILITE ; PROVENANCE ; GERMPLASM ; FRIJOL MUNGO ; VIGNA RADIATA RADIATA ; FOTOSENSIBILIDAD ; PROCEDENCIA ; GERMOPLASMA ; http://www.fao.org/aos/agrovoc#c_4988 ; http://www.fao.org/aos/agrovoc#c_8243 ; http://www.fao.org/aos/agrovoc#c_5811 ; http://www.fao.org/aos/agrovoc#c_16022 ; http://www.fao.org/aos/agrovoc#c_3249
    Language English
    Document type Article
    ISSN 0250-5371
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  6. Article: Identification and Characterization of Green Gram [Vigna radiata (L.) R. Wilczek.] Photosensitive Lines and Their Significance

    Sunil, N / Varaprasad, K.S / Sivaraj, N / Abraham, Babu / Kumar, Vinod

    Legume research. 2012 Mar., v. 35, no. 1

    2012  

    Abstract: One hundred and forty four accessions of green gram germplasm collected from middle Eastern Ghats were characterized and evaluated for 22 qualitative and quantitative traits during post rainy season of 2006–07 and rainy season of 2007. Three accessions ( ... ...

    Abstract One hundred and forty four accessions of green gram germplasm collected from middle Eastern Ghats were characterized and evaluated for 22 qualitative and quantitative traits during post rainy season of 2006–07 and rainy season of 2007. Three accessions (IC546478, IC546486 and IC369703) exhibited primitive characters such as photosensitivity, spreading growth habit and slight twining tendency, which differed significantly in comparison with the released varieties. Accession IC369703 was found promising for the quantitative traits viz. plant height (21.2 cm), primary branches (4.2) and pod clusters per plant (10.2) as against released varieties values ranging from 27–31.6cm, 1.4–2.4, 7.8–9.4 respectively. Based on the present study and previously reported archaeological evidences, it is proposed that green gram could have its origin in the cusp region of inner Western Ghats and inner Eastern Ghats.
    Keywords Vigna radiata ; archaeology ; branches ; germplasm ; growth habit ; mung beans ; photosensitivity ; plant height ; quantitative traits ; research ; varieties ; wet season
    Language English
    Dates of publication 2012-03
    Size p. 78-80.
    Publishing place Agricultural Research Communication Centre
    Document type Article
    Note NAL-light
    ISSN 0976-0571
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Ophthalmology Practice in the UK.

    Sivaraj, Ramesh

    Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)

    2020  Volume 9, Issue 5, Page(s) 477

    MeSH term(s) Humans ; Ophthalmology/trends ; Quality Improvement ; Surveys and Questionnaires ; United Kingdom
    Language English
    Publishing date 2020-09-01
    Publishing country United States
    Document type Letter
    ZDB-ID 2756329-7
    ISSN 2162-0989 ; 2162-0989
    ISSN (online) 2162-0989
    ISSN 2162-0989
    DOI 10.1097/APO.0000000000000318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Exploring the heat transfer and entropy generation of Ag/Fe[Formula: see text]O[Formula: see text]-blood nanofluid flow in a porous tube: a collocation solution.

    Basha, H Thameem / Sivaraj, R

    The European physical journal. E, Soft matter

    2021  Volume 44, Issue 3, Page(s) 31

    Abstract: Evaluating the entropy generation is essential in thermal systems to avoid the unnecessarily wasted thermal energy during the thermal processes. Nowadays, researchers are greatly fascinated to scrutinize the entropy generation in a human system because ... ...

    Abstract Evaluating the entropy generation is essential in thermal systems to avoid the unnecessarily wasted thermal energy during the thermal processes. Nowadays, researchers are greatly fascinated to scrutinize the entropy generation in a human system because it is utilized as a thermodynamic approach to understand the heat transfer characteristics of cancer systems or wounded tissue and their accessibility status. Further, numerous nanoparticles have been employed as an agent to control the heat transfer of blood and wounded tissue. As a result, the present model manifests the entropy generation, flow characteristics and heat transport of Ag/Fe[Formula: see text]O[Formula: see text]-blood flow of a nanofluid in a permeable circular tube with the influence of variable electrical conductivity and linear radiation. Nonlinear transport equations are converted into ordinary differential equations by suitable similarity variables which are solved with weighted residual method. Significant parameters like Reynolds number, dimensionless permeability parameter, extending/contracting parameter, Eckert number and Hartmann number on the radial pressure, axial velocity, radial velocity and temperature are explored through graphs. The obtained results show that temperature distribution of Fe[Formula: see text]O[Formula: see text] nanoparticles is higher than Ag nanoparticle, in case of suction. The dimensionless permeability parameter has an opposite nature on the radial pressure for the suction and injection cases. Growing values of Hartmann number enhance the total entropy generation for the cases of suction and injection.
    MeSH term(s) Entropy ; Hot Temperature ; Hydrodynamics ; Iron/chemistry ; Nanotechnology/instrumentation ; Porosity ; Silver/chemistry
    Chemical Substances Silver (3M4G523W1G) ; Iron (E1UOL152H7)
    Language English
    Publishing date 2021-03-15
    Publishing country France
    Document type Journal Article
    ZDB-ID 2004003-9
    ISSN 1292-895X ; 1292-8941
    ISSN (online) 1292-895X
    ISSN 1292-8941
    DOI 10.1140/epje/s10189-021-00024-x
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  9. Article ; Online: Screening for sickle-cell retinopathy.

    Ashwin, Pammal T / Sivaraj, Ramesh

    Eye (London, England)

    2022  Volume 37, Issue 2, Page(s) 375

    MeSH term(s) Humans ; Retinal Diseases/diagnosis ; Retinal Diseases/etiology ; Anemia, Sickle Cell/complications ; Anemia, Sickle Cell/diagnosis
    Language English
    Publishing date 2022-08-26
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 91001-6
    ISSN 1476-5454 ; 0950-222X
    ISSN (online) 1476-5454
    ISSN 0950-222X
    DOI 10.1038/s41433-022-02199-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 3010: Show issues Location:
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    Zs.MO 603: Show issues

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  10. Article: Molecular docking analysis of flavonoids with AChE and BACE-1.

    Viswanathan, Sittarthan / Arumugam, Thennavan / Subramanian, Kavimani / Sivaraj, Rengaraj / Ramesh, Vimalavathini / Vasanthi, A Hannah Rachel

    Bioinformation

    2024  Volume 20, Issue 2, Page(s) 103–109

    Abstract: Flavonoids are promising therapeutics for the treatment of Alzheimer's disease (AD). Therefore, it is of interest to study the anti-AD potential of 35 flavonoids towards the inhibition of AchE and BACE-1. Hence, the physicochemical, pharmacokinetic ... ...

    Abstract Flavonoids are promising therapeutics for the treatment of Alzheimer's disease (AD). Therefore, it is of interest to study the anti-AD potential of 35 flavonoids towards the inhibition of AchE and BACE-1. Hence, the physicochemical, pharmacokinetic parameters, toxicity risk and drug-likeliness of the selected 35 flavonoids were computed. Further, the molecular docking analysis of flavonoids with AChE and BACE-1 were completed. A binding energy of -10.42 kcal/mol Epicatechin gallate, -10.16 kcal/mol sterubin and -10.11 kcal/mol Fisetin was observed with AchE as potential inhibitors. Similarly, Biochainin-A -9.81kcal/mol, Sterubin -8.96 kcal/mol and Epicatechin gallate -7.4 7 kcal/mol showed with BACE-1. Thus, these flavonoids are potential leads for structure-based design of effective anti-Alzheimer's agents.
    Language English
    Publishing date 2024-02-29
    Publishing country Singapore
    Document type Journal Article
    ZDB-ID 2203786-X
    ISSN 0973-2063
    ISSN 0973-2063
    DOI 10.6026/973206300200103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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