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Article ; Online: Problems of Interpreting Diagnostic Tests for SARS-CoV-2: Analytical Chemistry Concerns.

Mattioli, Isabela A / Crespilho, Frank N

Anais da Academia Brasileira de Ciencias

2020 Volume 92, Issue 4, Page(s) e20201208

Abstract: The COVID-19 pandemic outbreak made the development of reliable, sensitive, and reproducible testing methods crucial throughout the world. Without proper analytical validation, testing results can be misinterpreted, leading to a certain degree of ... ...

Abstract	The COVID-19 pandemic outbreak made the development of reliable, sensitive, and reproducible testing methods crucial throughout the world. Without proper analytical validation, testing results can be misinterpreted, leading to a certain degree of misinformation in the clinical area. To accurately assess the methods, the determination of an analytical linear range of response of the assay is fundamental. Based on this curve, the evaluation of some parameters as sensitivity, limit of detection, and limit of quantification can be done, as well as the establishment of cut-off values. Statistical treatments of the collected data can be performed for reproducibility and reliability evaluations. In this context, there is a wide range of analytical concerns that should be in-depth discussed in medical, biomedical and chemical areas. This letter aims to briefly clarify some analytical chemistry concepts, as sensitivity, cut-off and limit of detection, and their application towards clinical diagnosis.
MeSH term(s)	Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Diagnostic Tests, Routine ; Humans ; Pandemics ; Pneumonia, Viral ; Reproducibility of Results ; SARS-CoV-2 ; Sensitivity and Specificity
Keywords	covid19
Language	English
Publishing date	2020-10-28
Publishing country	Brazil
Document type	Letter
ZDB-ID	2046885-4
ISSN	1678-2690 ; 0001-3765
ISSN (online)	1678-2690
ISSN	0001-3765
DOI	10.1590/0001-3765202020201208
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Problems of Interpreting Diagnostic Tests for SARS-CoV-2: Analytical Chemistry Concerns

Mattioli, Isabela A / Crespilho, Frank N

An Acad Bras Cienc

Abstract	The COVID-19 pandemic outbreak made the development of reliable, sensitive, and reproducible testing methods crucial throughout the world. Without proper analytical validation, testing results can be misinterpreted, leading to a certain degree of misinformation in the clinical area. To accurately assess the methods, the determination of an analytical linear range of response of the assay is fundamental. Based on this curve, the evaluation of some parameters as sensitivity, limit of detection, and limit of quantification can be done, as well as the establishment of cut-off values. Statistical treatments of the collected data can be performed for reproducibility and reliability evaluations. In this context, there is a wide range of analytical concerns that should be in-depth discussed in medical, biomedical and chemical areas. This letter aims to briefly clarify some analytical chemistry concepts, as sensitivity, cut-off and limit of detection, and their application towards clinical diagnosis.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #910273
Database	COVID19

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Article ; Online: Screen-printed disposable electrodes using graphite-polyurethane composites modified with magnetite and chitosan-coated magnetite nanoparticles for voltammetric epinephrine sensing: a comparative study.

Mattioli, Isabela A / Cervini, Priscila / Cavalheiro, Éder T G

Mikrochimica acta

2020 Volume 187, Issue 6, Page(s) 318

Abstract: Disposable screen-printed electrodes based on the use of graphite-polyurethane composites modified with magnetite nanoparticles (MNP-SPE) or chitosan-coated magnetite nanoparticles (CHMNP-SPE) are described. The MNP and CHMNP were synthetized and ... ...

Abstract	Disposable screen-printed electrodes based on the use of graphite-polyurethane composites modified with magnetite nanoparticles (MNP-SPE) or chitosan-coated magnetite nanoparticles (CHMNP-SPE) are described. The MNP and CHMNP were synthetized and comparatively characterized by TEM, XRD, FTIR, and TGA/DTG. The MNP-SPE and CHMNP-SPE were characterized by SEM and EDX. After optimization of the MNP percentage in MNP-SPE, the materials were electrochemically characterized by cyclic voltammetry, EIS, and chronocoulometry. The electrodes were tested for their performance towards sensing of epinephrine (EP). The CHMNP-SPE is found to have better electrochemical responses in comparison to the MNP-SPE. This is assumed to be due to the chitosan coating which also protects the MNPs from oxidation under air and at different applied potential fields. The performances of the MNP-SPE and CHMNP-SPE were studied by DPV after optimization of equilibration time and DPV parameters. Response is linear in the 0.1-0.8 μM EP concentration range, at 0.03 V (vs. pseudo-Ag/AgCl), and the detection limit is 25 nM for the MNP-SPE. The linear response for the CHMNP-SPE was 0.1-0.6 μM, at 0.0 V (vs. pseudo-Ag/AgCl), and a LOD of 14 nM was achieved. The devices were used for the quantification of EP in synthetic urine and in cerebrospinal synthetic fluids. Recoveries from spiked samples are in the 95.6-102.2% range for the CHMNP-SPE and in the 98.3-109% range for MNP-SPE. The stability of the respective sensors was investigated and compared over a period of 5 months. The EP peak currents were found to decrease by only 4% for the CHMNP-SPE, while the MNP-SPE lost 23% of its EP peak current. Accordingly, the CHMNP-SPE was chosen as the most stable and sensitive sensor for EP. Graphical abstract Schematic figure of modification of a graphite-polyurethane screen-printed composite electrode with magnetite nanoparticles (MNPs) and chitosan-coated magnetite nanoparticles (CHMNPs) for the voltammetric determination of epinephrine (EP). Improved response of CHMNP-SPE (black voltammogram) in comparison to MNP-SPE (red voltammogram) was attributed to the protection of MNP from oxidation.
MeSH term(s)	Chitosan/chemistry ; Electrochemical Techniques/instrumentation ; Electrochemical Techniques/methods ; Electrodes ; Epinephrine/chemistry ; Epinephrine/urine ; Graphite/chemistry ; Limit of Detection ; Magnetite Nanoparticles/chemistry ; Oxidation-Reduction ; Polyurethanes/chemistry
Chemical Substances	Magnetite Nanoparticles ; Polyurethanes ; Graphite (7782-42-5) ; Chitosan (9012-76-4) ; Epinephrine (YKH834O4BH)
Language	English
Publishing date	2020-05-10
Publishing country	Austria
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-020-04259-x
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Book ; Online: Problems of Interpreting Diagnostic Tests for SARS-CoV-2

MATTIOLI, ISABELA A. / CRESPILHO, FRANK N.

Anais da Academia Brasileira de Ciências v.92 n.4 2020

Analytical Chemistry Concerns

2020

Abstract: Abstract The COVID-19 pandemic outbreak made the development of reliable, sensitive, and reproducible testing methods crucial throughout the world. Without proper analytical validation, testing results can be misinterpreted, leading to a certain degree ... ...

Abstract	Abstract The COVID-19 pandemic outbreak made the development of reliable, sensitive, and reproducible testing methods crucial throughout the world. Without proper analytical validation, testing results can be misinterpreted, leading to a certain degree of misinformation in the clinical area. To accurately assess the methods, the determination of an analytical linear range of response of the assay is fundamental. Based on this curve, the evaluation of some parameters as sensitivity, limit of detection, and limit of quantification can be done, as well as the establishment of cut-off values. Statistical treatments of the collected data can be performed for reproducibility and reliability evaluations. In this context, there is a wide range of analytical concerns that should be in-depth discussed in medical, biomedical and chemical areas. This letter aims to briefly clarify some analytical chemistry concepts, as sensitivity, cut-off and limit of detection, and their application towards clinical diagnosis.
Keywords	tests ; COVID-19 ; Lateral Flow ; SARS-CoV-2 ; diagnosis ; covid19
Language	English
Publishing date	2020-01-01
Publisher	Academia Brasileira de Ciências
Publishing country	br
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Developing a screen-printed graphite-polyurethane composite electrode modified with gold nanoparticles for the voltammetric determination of dopamine.

Cervini, Priscila / Mattioli, Isabela A / Cavalheiro, Éder T G

RSC advances

2019 Volume 9, Issue 72, Page(s) 42306–42315

Abstract: A screen-printed electrode (SPGPUE) was prepared with graphite-polyurethane composite ink containing gold nanoparticles (AuNPs), resulting in a screen-printed graphite-polyurethane composite electrode modified with gold nanoparticles (SPGPUE-AuNPs). Gold ...

Abstract	A screen-printed electrode (SPGPUE) was prepared with graphite-polyurethane composite ink containing gold nanoparticles (AuNPs), resulting in a screen-printed graphite-polyurethane composite electrode modified with gold nanoparticles (SPGPUE-AuNPs). Gold nanoparticles were prepared by the citrate method and extracted from the water medium since polyurethane is not compatible with humidity. After extraction to chloroform, they were characterized
Language	English
Publishing date	2019-12-20
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/c9ra09046k
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies.

Mattioli, Isabela A / Hassan, Ayaz / Oliveira, Osvaldo N / Crespilho, Frank N

ACS sensors

2020 Volume 5, Issue 12, Page(s) 3655–3677

Abstract: Diagnosis of COVID-19 has been challenging owing to the need for mass testing and for combining distinct types of detection to cover the different stages of the infection. In this review, we have surveyed the most used methodologies for diagnosis of ... ...

Abstract	Diagnosis of COVID-19 has been challenging owing to the need for mass testing and for combining distinct types of detection to cover the different stages of the infection. In this review, we have surveyed the most used methodologies for diagnosis of COVID-19, which can be basically categorized into genetic-material detection and immunoassays. Detection of genetic material with real-time polymerase chain reaction (RT-PCR) and similar techniques has been achieved with high accuracy, but these methods are expensive and require time-consuming protocols which are not widely available, especially in less developed countries. Immunoassays for detecting a few antibodies, on the other hand, have been used for rapid, less expensive tests, but their accuracy in diagnosing infected individuals has been limited. We have therefore discussed the strengths and limitations of all of these methodologies, particularly in light of the required combination of tests owing to the long incubation periods. We identified the bottlenecks that prevented mass testing in many countries, and proposed strategies for further action, which are mostly associated with materials science and chemistry. Of special relevance are the methodologies which can be integrated into point-of-care (POC) devices and the use of artificial intelligence that do not require products from a well-developed biotech industry.
MeSH term(s)	Artificial Intelligence ; COVID-19/diagnosis ; COVID-19/virology ; Humans ; Immunoassay ; Point-of-Care Systems ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification
Language	English
Publishing date	2020-12-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ISSN	2379-3694
ISSN (online)	2379-3694
DOI	10.1021/acssensors.0c01382
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: From research to market: correlation between publications, patent filings, and investments in development and production of technological innovations in biosensors.

Cagnani, Giovana Rosso / da Costa Oliveira, Thiago / Mattioli, Isabela A / Sedenho, Graziela C / Castro, Karla P R / Crespilho, Frank N

Analytical and bioanalytical chemistry

2022 Volume 415, Issue 18, Page(s) 3645–3653

Abstract: As the global population grows and science and technology development evolve, fulfilling basic human needs has been even more linked to technological solutions. In this review, we present an overview of the biosensor market and discuss the factors that ... ...

Abstract	As the global population grows and science and technology development evolve, fulfilling basic human needs has been even more linked to technological solutions. In this review, we present an overview of the biosensor market and discuss the factors that make certain countries more competitive than others in terms of technology and innovation and how this is reflected in the trends in publication and patent filling. Additionally, we expose briefly how the COVID-19 pandemic acts as a catalyst for the integration of research and development, business, and innovation sectors to bring solutions and ideas that have been predicted as tendencies for the future.
MeSH term(s)	Humans ; Inventions ; Filing ; Pandemics ; COVID-19 ; Biosensing Techniques
Language	English
Publishing date	2022-12-07
Publishing country	Germany
Document type	Review ; Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-022-04444-2
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Article ; Online: Expanding the application of graphene vertical devices to dual femtomolar detection of SARS-CoV-2 receptor binding domain in serum and saliva.

Mattioli, Isabela A / Castro, Karla R / Sedenho, Graziela C / Macedo, Lucyano J A / Oliveira, Mona N / Manuli, Erika R / Sabino, Ester C / Crespilho, Frank N

Biosensors & bioelectronics

2023 Volume 239, Page(s) 115614

Abstract: The emergence of the graphene-based hybrid electrical-electrochemical vertical device (EEVD) has introduced a promising nanostructured biosensor tailored for point-of-care applications. In this study, we present an innovative EEVD capable of ... ...

Abstract	The emergence of the graphene-based hybrid electrical-electrochemical vertical device (EEVD) has introduced a promising nanostructured biosensor tailored for point-of-care applications. In this study, we present an innovative EEVD capable of simultaneously detecting the receptor binding domain (RBD) of the SARS-CoV-2 spike protein in both serum and saliva. The foundation of the EEVD lies in a poly-neutral red-graphene heterojunction, which has been enhanced with a bioconjugate of gold nanoparticles and antibodies. The biodevice demonstrates a remarkable limit of detection, registering at the femtomolar scale (2.86 fmol L
MeSH term(s)	Humans ; SARS-CoV-2 ; Saliva ; COVID-19/diagnosis ; Graphite ; Gold ; Biosensing Techniques ; Metal Nanoparticles
Chemical Substances	spike protein, SARS-CoV-2 ; Graphite (7782-42-5) ; Gold (7440-57-5)
Language	English
Publishing date	2023-08-18
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2023.115614
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Article ; Online: Highly sensitive interfaces of graphene electrical-electrochemical vertical devices for on drop atto-molar DNA detection.

Mattioli, Isabela A / Hassan, Ayaz / Sanches, Natalia M / Vieira, Nirton C S / Crespilho, Frank N

Biosensors & bioelectronics

2020 Volume 175, Page(s) 112851

Abstract: The development of novel high-sensitivity DNA-based biosensors is beneficial, as these devices have applications in the identification of genetic risk factors, medical diagnostics, and environmental monitoring. Herein, we report on the first robust ... ...

Abstract	The development of novel high-sensitivity DNA-based biosensors is beneficial, as these devices have applications in the identification of genetic risk factors, medical diagnostics, and environmental monitoring. Herein, we report on the first robust device capable of detecting DNA on a microliter drop with a zepto-molar (10
MeSH term(s)	Biosensing Techniques ; DNA/genetics ; DNA, Single-Stranded ; Graphite ; Molar
Chemical Substances	DNA, Single-Stranded ; Graphite (7782-42-5) ; DNA (9007-49-2)
Language	English
Publishing date	2020-11-24
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2020.112851
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Article: Graphene-based hybrid electrical-electrochemical point-of-care device for serologic COVID-19 diagnosis

Mattioli, Isabela A. / Castro, Karla R. / Macedo, Lucyano J.A. / Sedenho, Graziela C. / Oliveira, Mona N. / Todeschini, Iris / Vitale, Phelipe M. / Ferreira, Suzete Cleusa / Manuli, Erika R. / Pereira, Geovana M. / Sabino, Ester C. / Crespilho, Frank N.

Biosensors & bioelectronics. 2022 Mar. 01, v. 199

2022

Abstract: The outbreak of COVID-19 pandemics highlighted the need of sensitive, selective, and easy-to-handle biosensing devices. In the contemporary scenario, point-of-care devices for mass testing and infection mapping within a population have proven themselves ... ...

Abstract	The outbreak of COVID-19 pandemics highlighted the need of sensitive, selective, and easy-to-handle biosensing devices. In the contemporary scenario, point-of-care devices for mass testing and infection mapping within a population have proven themselves as of primordial importance. Here, we introduce a graphene-based Electrical-Electrochemical Vertical Device (EEVD) point-of-care biosensor, strategically engineered for serologic COVID-19 diagnosis. EEVD uses serologic IgG quantifications on SARS-CoV-2 Receptor Binding Domain (RBD) bioconjugate immobilized onto device surface. EEVD combines graphene basal plane with high charge carrier mobility, high conductivity, low intrinsic resistance, and interfacial sensitivity to capacitance alterations. EEVD application was carried out in real human serum samples. Since EEVD is a miniaturized device, it requires just 40 μL of sample for a point-of-care COVID-19 infections detection. When compared to serologic assays such ELISA and other immunochromatographic methods, EEVD presents some advantages such as time of analyses (15 min), sample preparation, and a LOD of 1.0 pg mL⁻¹. We glimpse that EEVD meets the principles of robustness and accuracy, desirable analytic parameters for assays destined to pandemics control strategies.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; biosensors ; blood serum ; capacitance ; graphene ; humans ; immunoaffinity chromatography ; point-of-care systems
Language	English
Dates of publication	2022-0301
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2021.113866
Database	NAL-Catalogue (AGRICOLA)

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