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Article ; Online: Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an Engineered Strain of

Pérez-Valero, Álvaro / Serna-Diestro, Juan / Villar, Claudio J / Lombó, Felipe

International journal of molecular sciences

2024 Volume 25, Issue 5

Abstract: Flavonoids are a large family of polyphenolic compounds with important agro-industrial, nutraceutical, and pharmaceutical applications. Among the structural diversity found in the flavonoid family, methylated flavonoids show interesting characteristics ... ...

Abstract	Flavonoids are a large family of polyphenolic compounds with important agro-industrial, nutraceutical, and pharmaceutical applications. Among the structural diversity found in the flavonoid family, methylated flavonoids show interesting characteristics such as greater stability and improved oral bioavailability. This work is focused on the reconstruction of the entire biosynthetic pathway of the methylated flavones diosmetin and chrysoeriol in
MeSH term(s)	3-Deoxy-7-Phosphoheptulonate Synthase/metabolism ; Phosphates ; Flavones/metabolism ; Flavonoids/chemistry ; Streptomyces
Chemical Substances	chrysoeriol (Q813145M20) ; diosmetin (TWZ37241OT) ; 3-Deoxy-7-Phosphoheptulonate Synthase (EC 2.5.1.54) ; Phosphates ; Flavones ; Flavonoids
Language	English
Publishing date	2024-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25052776
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Smart Polymer Surfaces with Complex Wrinkled Patterns: Reversible, Non-Planar, Gradient, and Hierarchical Structures.

Sarabia-Vallejos, Mauricio A / Cerda-Iglesias, Felipe E / Pérez-Monje, Dan A / Acuña-Ruiz, Nicolas F / Terraza-Inostroza, Claudio A / Rodríguez-Hernández, Juan / González-Henríquez, Carmen M

Polymers

2023 Volume 15, Issue 3

Abstract: This review summarizes the relevant developments in preparing wrinkled structures with variable characteristics. These include the formation of smart interfaces with reversible wrinkle formation, the construction of wrinkles in non-planar supports, or, ... ...

Abstract	This review summarizes the relevant developments in preparing wrinkled structures with variable characteristics. These include the formation of smart interfaces with reversible wrinkle formation, the construction of wrinkles in non-planar supports, or, more interestingly, the development of complex hierarchically structured wrinkled patterns. Smart wrinkled surfaces obtained using light-responsive, pH-responsive, temperature-responsive, and electromagnetic-responsive polymers are thoroughly described. These systems control the formation of wrinkles in particular surface positions and the reversible construction of planar-wrinkled surfaces. This know-how of non-planar substrates has been recently extended to other structures, thus forming wrinkled patterns on solid, hollow spheres, cylinders, and cylindrical tubes. Finally, this bibliographic analysis also presents some illustrative examples of the potential of wrinkle formation to create more complex patterns, including gradient structures and hierarchically multiscale-ordered wrinkles. The orientation and the wrinkle characteristics (amplitude and period) can also be modulated according to the requested application.
Language	English
Publishing date	2023-01-25
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym15030612
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Article ; Online: Minicells as an Escherichia coli mechanism for the accumulation and disposal of fluorescent cadmium sulphide nanoparticles.

Valenzuela-Ibaceta, Felipe / Torres-Olea, Nicolás / Ramos-Zúñiga, Javiera / Dietz-Vargas, Claudio / Navarro, Claudio A / Pérez-Donoso, José M

Journal of nanobiotechnology

2024 Volume 22, Issue 1, Page(s) 78

Abstract: Background: Bacterial biosynthesis of fluorescent nanoparticles or quantum dots (QDs) has emerged as a unique mechanism for heavy metal tolerance. However, the physiological pathways governing the removal of QDs from bacterial cells remains elusive. ... ...

Abstract	Background: Bacterial biosynthesis of fluorescent nanoparticles or quantum dots (QDs) has emerged as a unique mechanism for heavy metal tolerance. However, the physiological pathways governing the removal of QDs from bacterial cells remains elusive. This study investigates the role of minicells, previously identified as a means of eliminating damaged proteins and enhancing bacterial resistance to stress. Building on our prior work, which unveiled the formation of minicells during cadmium QDs biosynthesis in Escherichia coli, we hypothesize that minicells serve as a mechanism for the accumulation and detoxification of QDs in bacterial cells. Results: Intracellular biosynthesis of CdS QDs was performed in E. coli mutants ΔminC and ΔminCDE, known for their minicell-producing capabilities. Fluorescence microscopy analysis demonstrated that the generated minicells exhibited fluorescence emission, indicative of QD loading. Transmission electron microscopy (TEM) confirmed the presence of nanoparticles in minicells, while energy dispersive spectroscopy (EDS) revealed the coexistence of cadmium and sulfur. Cadmium quantification through flame atomic absorption spectrometry (FAAS) demonstrated that minicells accumulated a higher cadmium content compared to rod cells. Moreover, fluorescence intensity analysis suggested that minicells accumulated a greater quantity of fluorescent nanoparticles, underscoring their efficacy in QD removal. Biosynthesis dynamics in minicell-producing strains indicated that biosynthesized QDs maintained high fluorescence intensity even during prolonged biosynthesis times, suggesting continuous QD clearance in minicells. Conclusions: These findings support a model wherein E. coli utilizes minicells for the accumulation and removal of nanoparticles, highlighting their physiological role in eliminating harmful elements and maintaining cellular fitness. Additionally, this biosynthesis system presents an opportunity for generating minicell-coated nanoparticles with enhanced biocompatibility for diverse applications.
MeSH term(s)	Escherichia coli/metabolism ; Cadmium ; Nanoparticles/chemistry ; Quantum Dots/chemistry ; Coloring Agents/metabolism ; Sulfides ; Cadmium Compounds
Chemical Substances	cadmium sulfide (057EZR4Z7Q) ; Cadmium (00BH33GNGH) ; Coloring Agents ; Sulfides ; Cadmium Compounds
Language	English
Publishing date	2024-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2100022-0
ISSN	1477-3155 ; 1477-3155
ISSN (online)	1477-3155
ISSN	1477-3155
DOI	10.1186/s12951-024-02348-0
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Article ; Online: Incidence and case-fatality rate of stroke in General Villegas, Buenos Aires, Argentina. The EstEPA population study.

Ameriso, Sebastián F / Alet, Matías J / Rosales, Julieta / Rodríguez-Pérez, María S / Povedano, Guillermo P / Pujol-Lereis, Virginia A / Rodríguez-Lucci, Federico / Dossi, Daiana / González, Claudio D / Melcon, Mario O

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association

2023 Volume 32, Issue 5, Page(s) 107058

Abstract: Objectives: Stroke epidemiology varies among different populations. The burden of stroke is high in low- and middle-income countries. Reliable population data is needed to assess the impact of stroke and to develop policies aimed to improve stroke care ... ...

Abstract	Objectives: Stroke epidemiology varies among different populations. The burden of stroke is high in low- and middle-income countries. Reliable population data is needed to assess the impact of stroke and to develop policies aimed to improve stroke care in our region. EstEPA is a population-based project assessing prevalence, incidence, mortality and burden of stroke in General Villegas Department, Buenos Aires, Argentina (pop=30,864 inhabitants). We determined incidence of stroke (first-ever and recurrent stroke) and stroke case-fatality rate from 2017 to 2020. Methods: First-ever strokes, recurrent strokes and transient ischemic attacks were ascertained and case-fatality rate was obtained. Diagnoses were based on standard AHA/WHO definitions. Study population included all persons residing in General Villegas during the three-year period. Hospitals, households, nursing homes, death certificates and several overlapping sources were surveyed. Results: We assessed 92,592 person-years. There were 155 cerebrovascular events aged 70 years (SD ± 13 years), of which 115 were first-ever strokes (74%), 21 recurrent strokes (13.5%) and 19 transient ischemic attacks (12.5%). The crude overall incidence rate of first-ever strokes was 124.2 per 100,000 population (86.9 per 100,000 [95% CI 58.5-115.2] when standardized by WHO World population and 109.7 per 100,000 [95% CI 89.7-129.8] when standardized by Argentine population) and 317.0 per 100,000 population in subjects older than 40 years. Case fatality rate at 30 days of first-ever strokes was 27%. Conclusion: In this population-based comprehensive stroke epidemiological study in Argentina, first-ever stroke incidence in an urban population was 124.2 per 100,000 population (86.9 per 100,000 adjusted by the WHO World population). This is lower than the incidence in other countries in the region and similar to a recent incidence study in Argentina. It is also comparable to reported incidence in most middle- and high-income countries. Stroke case-fatality rate was comparable to other population-based Latin-American studies.
MeSH term(s)	Humans ; Ischemic Attack, Transient/diagnosis ; Ischemic Attack, Transient/epidemiology ; Incidence ; Argentina/epidemiology ; Stroke/diagnosis ; Stroke/epidemiology ; Nursing Homes
Language	English
Publishing date	2023-03-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	1131675-5
ISSN	1532-8511 ; 1052-3057
ISSN (online)	1532-8511
ISSN	1052-3057
DOI	10.1016/j.jstrokecerebrovasdis.2023.107058
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Article ; Online: Color–Texture Pattern Classification Using Global–Local Feature Extraction, an SVM Classifier, with Bagging Ensemble Post-Processing

Carlos F. Navarro / Claudio A. Perez

Applied Sciences, Vol 9, Iss 15, p

2019 Volume 3130

Abstract: Many applications in image analysis require the accurate classification of complex patterns including both color and texture, e.g., in content image retrieval, biometrics, and the inspection of fabrics, wood, steel, ceramics, and fruits, among others. A ... ...

Abstract	Many applications in image analysis require the accurate classification of complex patterns including both color and texture, e.g., in content image retrieval, biometrics, and the inspection of fabrics, wood, steel, ceramics, and fruits, among others. A new method for pattern classification using both color and texture information is proposed in this paper. The proposed method includes the following steps: division of each image into global and local samples, texture and color feature extraction from samples using a Haralick statistics and binary quaternion-moment-preserving method, a classification stage using support vector machine, and a final stage of post-processing employing a bagging ensemble. One of the main contributions of this method is the image partition, allowing image representation into global and local features. This partition captures most of the information present in the image for colored texture classification allowing improved results. The proposed method was tested on four databases extensively used in color−texture classification: the Brodatz, VisTex, Outex, and KTH-TIPS2b databases, yielding correct classification rates of 97.63%, 97.13%, 90.78%, and 92.90%, respectively. The use of the post-processing stage improved those results to 99.88%, 100%, 98.97%, and 95.75%, respectively. We compared our results to the best previously published results on the same databases finding significant improvements in all cases.
Keywords	colored texture pattern classification ; global–local texture classification ; color–texture features ; color–texture feature extraction ; bagging post-processing ; BQMP and Haralick global–local feature integration ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	006
Language	English
Publishing date	2019-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Perilesional edema diameter associated with brain metastases as a predictive factor of response to radiotherapy in non-small cell lung cancer.

Arrieta, Oscar / Bolaño-Guerra, Laura Margarita / Caballé-Pérez, Enrique / Lara-Mejía, Luis / Turcott, Jenny G / Gutiérrez, Salvador / Lozano-Ruiz, Francisco / Cabrera-Miranda, Luis / Arroyave-Ramírez, Andrés Mauricio / Maldonado-Magos, Federico / Corrales, Luis / Martín, Claudio / Gómez-García, Ana Pamela / Cacho-Díaz, Bernardo / Cardona, Andrés F

Frontiers in oncology

2023 Volume 13, Page(s) 1251620

Abstract: Background: Different prognostic scales exist in patients with brain metastasis, particularly in lung cancer. The Graded Prognostic Assessment for lung cancer using molecular markers (Lung-molGPA index) for brain metastases is a powerful prognostic tool ...

Abstract	Background: Different prognostic scales exist in patients with brain metastasis, particularly in lung cancer. The Graded Prognostic Assessment for lung cancer using molecular markers (Lung-molGPA index) for brain metastases is a powerful prognostic tool that effectively identifies patients at different risks. However, these scales do not include perilesional edema diameter (PED) associated with brain metastasis. Current evidence suggests that PED might compromise the delivery and efficacy of radiotherapy to treat BM. This study explored the association between radiotherapy efficacy, PED extent, and gross tumor diameter (GTD). Aim: The aim of this study was to evaluate the intracranial response (iORR), intracranial progression-free survival (iPFS), and overall survival (OS) according to the extent of PED and GT. Methods: Out of 114 patients with BM at baseline or throughout the disease, 65 were eligible for the response assessment. The GTD and PED sum were measured at BM diagnosis and after radiotherapy treatment. According to a receiver operating characteristic (ROC) curve analysis, cutoff values were set at 27 mm and 17 mm for PED and GT, respectively. Results: Minor PED was independently associated with a better iORR [78.8% vs. 50%, OR 3.71 (95% CI 1.26-10.99); Conclusion: Higher PED was associated with an increased risk of intracranial progression and a lesser probability of responding to brain radiotherapy in patients with metastatic lung cancer. We encourage prospective studies to confirm our findings.
Language	English
Publishing date	2023-10-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1251620
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Article ; Online: Smart Polymer Surfaces with Complex Wrinkled Patterns

Mauricio A. Sarabia-Vallejos / Felipe E. Cerda-Iglesias / Dan A. Pérez-Monje / Nicolas F. Acuña-Ruiz / Claudio A. Terraza-Inostroza / Juan Rodríguez-Hernández / Carmen M. González-Henríquez

Polymers, Vol 15, Iss 612, p

Reversible, Non-Planar, Gradient, and Hierarchical Structures

2023 Volume 612

Abstract	This review summarizes the relevant developments in preparing wrinkled structures with variable characteristics. These include the formation of smart interfaces with reversible wrinkle formation, the construction of wrinkles in non-planar supports, or, more interestingly, the development of complex hierarchically structured wrinkled patterns. Smart wrinkled surfaces obtained using light-responsive, pH-responsive, temperature-responsive, and electromagnetic-responsive polymers are thoroughly described. These systems control the formation of wrinkles in particular surface positions and the reversible construction of planar-wrinkled surfaces. This know-how of non-planar substrates has been recently extended to other structures, thus forming wrinkled patterns on solid, hollow spheres, cylinders, and cylindrical tubes. Finally, this bibliographic analysis also presents some illustrative examples of the potential of wrinkle formation to create more complex patterns, including gradient structures and hierarchically multiscale-ordered wrinkles. The orientation and the wrinkle characteristics (amplitude and period) can also be modulated according to the requested application.
Keywords	wrinkles surface ; surface microstructuration ; smart interfaces ; non-planar structuration ; hierarchical surface structures ; Organic chemistry ; QD241-441
Subject code	600
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Spatio-temporal patterns of the crustacean demersal fishery discard from the south Humboldt Current System, based on scientific observer program (2014-2019).

Landaeta, Mauricio F / Hernández-Santoro, Carola / Search, Francesca V / Castillo, Manuel I / Bernal, Claudio / Navarrete, Sergio A / Wieters, Evie A / Beldade, Ricardo / Navarro Campoi, Ana / Pérez-Matus, Alejandro

PloS one

2023 Volume 18, Issue 2, Page(s) e0281932

Abstract: This study summarises six years of spatio-temporal patterns of the discarded demersal community fauna recorded by onboard scientific observer program for both artisanal and industrial crustacean fisheries between 2014 and 2019, from mesophotic to aphotic ...

Abstract	This study summarises six years of spatio-temporal patterns of the discarded demersal community fauna recorded by onboard scientific observer program for both artisanal and industrial crustacean fisheries between 2014 and 2019, from mesophotic to aphotic depths (96 to 650 m) along the southern Humboldt Current System (28-38°S). In this period, one cold and two warm climatic events were observed during the austral summer 2014, 2015-2016 (ENSO Godzilla), and 2016-2017 (coastal ENSO), respectively. Satellite information showed that Chlorophyll-a concentration varied seasonally and latitudinally, associated with upwelling centres, while equatorial wind stress decreased southward of 36°S. Discards were composed of 108 species, dominated by finfish and molluscs. The Chilean hake Merluccius gayi was dominant and ubiquitous (occurrence, 95% of 9104 hauls), being the most vulnerable species of the bycatch. Three assemblages were identified: assemblage 1 (~200 m deep), dominated by flounders Hippoglossina macrops and lemon crabs Platymera gaudichaudii, assemblage 2 (~260 m deep), dominated by squat lobsters Pleuroncodes monodon and Cervimunida johni and assemblage 3 (~320 m depth), dominated by grenadiers Coelorinchus aconcagua and cardinalfish Epigonus crassicaudus. These assemblages were segregated by depth, and varied by year, and geographic zone. The latter represented changes in the width of the continental shelf, increasing southward of 36°S. Alpha-diversity indexes (richness, Shannon, Simpson, and Pielou) also varied with depth and latitude, with higher diversity in deeper continental waters (>300 m), between 2018-2019. Finally, at a spatial scale of tens of kilometres, and a monthly basis, interannual variations of biodiversity occurred in the demersal community. Surface sea temperature, chlorophyll-a, or wind stress did not correlate with discarded demersal fauna diversity of the crustacean fishery operating along central Chile.
MeSH term(s)	Animals ; Fisheries ; Anomura ; Biodiversity ; Chile ; Chlorophyll ; Chlorophyll A ; Flounder ; Gadiformes
Chemical Substances	Chlorophyll (1406-65-1) ; Chlorophyll A (YF5Q9EJC8Y)
Language	English
Publishing date	2023-02-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0281932
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: On the footsteps of Triadin and its role in skeletal muscle.

Perez, Claudio F

World journal of biological chemistry

2011 Volume 2, Issue 8, Page(s) 177–183

Abstract: Calcium is a crucial element for striated muscle function. As such, myoplasmic free Ca(2+) concentration is delicately regulated through the concerted action of multiple Ca(2+) pathways that relay excitation of the plasma membrane to the intracellular ... ...

Abstract	Calcium is a crucial element for striated muscle function. As such, myoplasmic free Ca(2+) concentration is delicately regulated through the concerted action of multiple Ca(2+) pathways that relay excitation of the plasma membrane to the intracellular contractile machinery. In skeletal muscle, one of these major Ca(2+) pathways is Ca(2+) release from intracellular Ca(2+) stores through type-1 ryanodine receptor/Ca(2+) release channels (RyR1), which positions RyR1 in a strategic cross point to regulate Ca(2+) homeostasis. This major Ca(2+) traffic point appears to be highly sensitive to the intracellular environment, which senses through a plethora of chemical and protein-protein interactions. Among these modulators, perhaps one of the most elusive is Triadin, a muscle-specific protein that is involved in many crucial aspect of muscle function. This family of proteins mediates complex interactions with various Ca(2+) modulators and seems poised to be a relevant modulator of Ca(2+) signaling in cardiac and skeletal muscles. The purpose of this review is to examine the most recent evidence and current understanding of the role of Triadin in muscle function, in general, with particular emphasis on its contribution to Ca(2+) homeostasis.
Language	English
Publishing date	2011-09-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2564793-3
ISSN	1949-8454 ; 1949-8454
ISSN (online)	1949-8454
ISSN	1949-8454
DOI	10.4331/wjbc.v2.i8.177
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Article ; Online: Spatio-temporal patterns of the crustacean demersal fishery discard from the south Humboldt Current System, based on scientific observer program (2014-2019).

Mauricio F Landaeta / Carola Hernández-Santoro / Francesca V Search / Manuel I Castillo / Claudio Bernal / Sergio A Navarrete / Evie A Wieters / Ricardo Beldade / Ana Navarro Campoi / Alejandro Pérez-Matus

PLoS ONE, Vol 18, Iss 2, p e

2023 Volume 0281932

Abstract	This study summarises six years of spatio-temporal patterns of the discarded demersal community fauna recorded by onboard scientific observer program for both artisanal and industrial crustacean fisheries between 2014 and 2019, from mesophotic to aphotic depths (96 to 650 m) along the southern Humboldt Current System (28-38°S). In this period, one cold and two warm climatic events were observed during the austral summer 2014, 2015-2016 (ENSO Godzilla), and 2016-2017 (coastal ENSO), respectively. Satellite information showed that Chlorophyll-a concentration varied seasonally and latitudinally, associated with upwelling centres, while equatorial wind stress decreased southward of 36°S. Discards were composed of 108 species, dominated by finfish and molluscs. The Chilean hake Merluccius gayi was dominant and ubiquitous (occurrence, 95% of 9104 hauls), being the most vulnerable species of the bycatch. Three assemblages were identified: assemblage 1 (~200 m deep), dominated by flounders Hippoglossina macrops and lemon crabs Platymera gaudichaudii, assemblage 2 (~260 m deep), dominated by squat lobsters Pleuroncodes monodon and Cervimunida johni and assemblage 3 (~320 m depth), dominated by grenadiers Coelorinchus aconcagua and cardinalfish Epigonus crassicaudus. These assemblages were segregated by depth, and varied by year, and geographic zone. The latter represented changes in the width of the continental shelf, increasing southward of 36°S. Alpha-diversity indexes (richness, Shannon, Simpson, and Pielou) also varied with depth and latitude, with higher diversity in deeper continental waters (>300 m), between 2018-2019. Finally, at a spatial scale of tens of kilometres, and a monthly basis, interannual variations of biodiversity occurred in the demersal community. Surface sea temperature, chlorophyll-a, or wind stress did not correlate with discarded demersal fauna diversity of the crustacean fishery operating along central Chile.
Keywords	Medicine ; R ; Science ; Q
Subject code	551
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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