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  1. Book ; Online: Nutritional and anti-methanogenic potentials of macroalgae for ruminants

    Pandey, Deepak / Mansouryar, Morteza / Novoa-Garrido, Margarita / Næss, Geir / Kiron, Viswanath / Hansen, Hanne / Nielsen, Mette Olaf / Khanal, Prabhat

    (Burleigh Dodds Series in Agricultural Science)

    2021  

    Series title Burleigh Dodds Series in Agricultural Science
    Keywords Aquaculture & fish-farming: practice & techniques ; Sustainable agriculture ; Phycology, algae & lichens ; digestibility ; fermentation ; methane ; seaweeds ; secondary metabolites
    Language English
    Size 1 electronic resource (34 pages)
    Publisher Burleigh Dodds Science Publishing
    Publishing place Cambridge
    Document type Book ; Online
    Note English
    HBZ-ID HT030375934
    ISBN 9781786768919 ; 1786768917
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Dose- and substrate-dependent reduction of enteric methane and ammonia by natural additives

    Battelli, Marco / Nielsen, Mette Olaf / Nørskov, Natalja P

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1302346

    Abstract: Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane ( ... ...

    Abstract Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH
    Language English
    Publishing date 2023-11-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1302346
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Dose- and substrate-dependent reduction of enteric methane and ammonia by natural additives in vitro

    Marco Battelli / Mette Olaf Nielsen / Natalja P. Nørskov

    Frontiers in Veterinary Science, Vol

    2023  Volume 10

    Abstract: Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH4) which is a product of rumen fermentation. The use of feed additives able to modulate rumen fermentation is a promising strategy to reduce enteric CH4 and ... ...

    Abstract Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH4) which is a product of rumen fermentation. The use of feed additives able to modulate rumen fermentation is a promising strategy to reduce enteric CH4 and ammonia (NH3) emissions. Among the various strategies investigated, plant secondary metabolites (PSMs) have attracted attention due to their apparent potential to reduce enteric CH4 and NH3 emissions, and it would be possible to use such compounds as feed additives in organic production systems. In an in vitro system simulating rumen fermentation, we have tested the impact of different classes of naturally occurring PSMs; catechin and quercetin (flavonoids), salicylic acid (phenolic acid) and tannic acid (hydrolysable tannin). The PSMs were added to two different basal feeds (maize and grass silages) at three inclusion doses 1.5, 3 and 6% of the feed dry matter (DM). CH4 production was significantly lowered upon addition of quercetin to two basal feeds at doses of 3 and 6%, and this without changes in concentrations of total volatile fatty acid (VFA) produced during fermentation. Quercetin, as the only tested additive, reduced CH4 production, and when added to maize silage and grass silage, the reduction increased linearly with increasing dose, ie., by 51 and 43%, respectively, at a dose of 3% of feed DM and by 86 and 58%, respectively, at a dose of 6% of feed DM. Moreover, quercetin significantly reduced NH3 concentration by >12% at doses of 3 and 6% in feed DM irrespective of the basal feed used as compared to when the basal feeds were incubated alone. Although none of the other additives affected CH4 formation, several additives had significant impacts on concentrations of NH3 and VFAs in the incubated fluid after fermentation. This study demonstrated a dose-dependent ability of quercetin to reduce CH4 emission from rumen fermentation, however, the magnitude of the suppression of CH4 depended on the basal feed. Furthermore, quercetin reduced NH3 concentration ...
    Keywords rumen fermentation ; plant secondary metabolites ; quercetin ; catechin ; salicylic acid ; tannic acid ; Veterinary medicine ; SF600-1100
    Subject code 660 ; 571
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Synchronization of S Phase in Schizosaccharomyces pombe Cells by Transient Exposure to M-Factor Pheromone.

    Nielsen, Olaf

    Cold Spring Harbor protocols

    2016  Volume 2016, Issue 9, Page(s) pdb.prot091272

    Abstract: A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a ... ...

    Abstract A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.
    MeSH term(s) DNA Replication ; Pheromones/metabolism ; S Phase/drug effects ; Schizosaccharomyces/drug effects ; Schizosaccharomyces/growth & development ; Schizosaccharomyces/metabolism
    Chemical Substances Pheromones
    Language English
    Publishing date 2016-09-01
    Publishing country United States
    Document type Journal Article
    ISSN 1559-6095
    ISSN (online) 1559-6095
    DOI 10.1101/pdb.prot091272
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book: Metabolic flux analysis

    Krömer, Jens Olaf / Nielsen, Lars K. / Blank, Lars Mathias

    methods and protocols

    (Methods in molecular biology ; 1191 ; Springer protocols)

    2014  

    Author's details ed. by Jens O. Krömer ; Lars K. Nielsen ; Lars M. Blank
    Series title Methods in molecular biology ; 1191
    Springer protocols
    Collection
    Keywords Cell metabolism
    Subject code 571.4
    Language English
    Size XII, 316 S. : Ill., graph. Darst., 27 cm
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Formerly CIP. ; Includes bibliographical references and index
    HBZ-ID HT018416528
    ISBN 978-1-4939-1169-1 ; 9781493911707 ; 1-4939-1169-4 ; 1493911708
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1191- verfügbar in Königswinter Königswinter

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  6. Article: Is Foetal Programming by Mismatched Pre- and Postnatal Nutrition Contributing to the Prevalence of Obesity in Nepal?

    Khanal, Prabhat / Nielsen, Mette Olaf

    Preventive nutrition and food science

    2019  Volume 24, Issue 3, Page(s) 235–244

    Abstract: Nepal and many developing countries are currently suffering from increased prevalence of obesity, type 2 diabetes, and other metabolic disorders. Unhealthy dietary habits and physical inactivity are traditionally considered as responsible factors for ... ...

    Abstract Nepal and many developing countries are currently suffering from increased prevalence of obesity, type 2 diabetes, and other metabolic disorders. Unhealthy dietary habits and physical inactivity are traditionally considered as responsible factors for these disorders. The relatively new concept of foetal programming suggests that development of metabolic diseases later in life may be associated with poor nutritional status
    Language English
    Publishing date 2019-09-30
    Publishing country Korea (South)
    Document type Journal Article ; Review
    ZDB-ID 2668373-8
    ISSN 2287-8602 ; 2287-1098
    ISSN (online) 2287-8602
    ISSN 2287-1098
    DOI 10.3746/pnf.2019.24.3.235
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 7808: Show issues

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  7. Article ; Online: Dose- and substrate-dependent reduction of enteric methane and ammonia by natural additives in vitro

    Nørskov, Natalja P. / Nielsen, Mette Olaf / Battelli, Marco

    2023  

    Abstract: Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH4) which is a product of rumen fermentation. The use of feed additives able to modulate rumen fermentation is a promising strategy to reduce enteric CH4 and ... ...

    Abstract Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH4) which is a product of rumen fermentation. The use of feed additives able to modulate rumen fermentation is a promising strategy to reduce enteric CH4 and ammonia (NH3) emissions. Among the various strategies investigated, plant secondary metabolites (PSMs) have attracted attention due to their apparent potential to reduce enteric CH4 and NH3 emissions, and it would be possible to use such compounds as feed additives in organic production systems. In an in vitro system simulating rumen fermentation, we have tested the impact of different classes of naturally occurring PSMs; catechin and quercetin (flavonoids), salicylic acid (phenolic acid) and tannic acid (hydrolysable tannin). The PSMs were added to two different basal feeds (maize and grass silages) at three inclusion doses 1.5, 3 and 6% of the feed dry matter (DM). CH4 production was significantly lowered upon addition of quercetin to two basal feeds at doses of 3 and 6%, and this without changes in concentrations of total volatile fatty acid (VFA) produced during fermentation. Quercetin, as the only tested additive, reduced CH4 production, and when added to maize silage and grass silage, the reduction increased linearly with increasing dose, ie., by 51 and 43%, respectively, at a dose of 3% of feed DM and by 86 and 58%, respectively, at a dose of 6% of feed DM. Moreover, quercetin significantly reduced NH3 concentration by >12% at doses of 3 and 6% in feed DM irrespective of the basal feed used as compared to when the basal feeds were incubated alone. Although none of the other additives affected CH4 formation, several additives had significant impacts on concentrations of NH3 and VFAs in the incubated fluid after fermentation. This study demonstrated a dose-dependent ability of quercetin to reduce CH4 emission from rumen fermentation, however, the magnitude of the suppression of CH4 depended on the basal feed. Furthermore, quercetin reduced NH3 concentration ...
    Keywords Dairy cattle
    Subject code 660
    Language English
    Publishing country dk
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Methane reduction by quercetin, tannic and salicylic acids: influence of molecular structures on methane formation and fermentation in vitro.

    Nørskov, Natalja P / Battelli, Marco / Curtasu, Mihai V / Olijhoek, Dana W / Chassé, Élisabeth / Nielsen, Mette Olaf

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 16023

    Abstract: Plant secondary metabolites (PSMs) can potentially reduce ruminal methane formation. However, related to differences in their molecular structures, it is not yet clear what causes an anti-methanogenic effect. In an in vitro system simulating rumen ... ...

    Abstract Plant secondary metabolites (PSMs) can potentially reduce ruminal methane formation. However, related to differences in their molecular structures, it is not yet clear what causes an anti-methanogenic effect. In an in vitro system simulating rumen fermentation, we investigated the impact of eight compounds with distinct chemical characteristics (gallic and salicylic acids, tannic acid, catechin, epicatechin, quercetin, rutin, and salicin) when added to a basal feed (maize silage) at a concentration of 12% of the feed dry matter. After 48 h of incubation in buffered rumen fluid, methane production was significantly lowered by quercetin (43%), tannic acid (39%) and salicylic acid (34%) compared to the control (maize silage alone) and without changes in total volatile fatty acid production during fermentation. No other PSM reduced methane formation as compared to control but induced significant differences on total volatile fatty acid production. The observed differences were related to lipophilicity, the presence of double bond and carbonyl group, sugar moieties, and polymerization of the compounds. Our results indicate the importance of distinct molecular structures of PSMs and chemical characteristics for methane lowering properties and volatile fatty acid formation. Further systematic screening studies to establish the structure-function relationship between PSMs and methane reduction are warranted.
    MeSH term(s) Animals ; Diet ; Quercetin/pharmacology ; Quercetin/metabolism ; Molecular Structure ; Fermentation ; Methane/metabolism ; Salicylates/pharmacology ; Rumen/metabolism ; Fatty Acids, Volatile/metabolism ; Zea mays/metabolism ; Tannins/pharmacology ; Tannins/metabolism ; Animal Feed/analysis
    Chemical Substances Quercetin (9IKM0I5T1E) ; Methane (OP0UW79H66) ; Salicylates ; Fatty Acids, Volatile ; Tannins
    Language English
    Publishing date 2023-09-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-43041-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep.

    Ahmad, Sharmila / Drag, Markus Hodal / Mohamad Salleh, Suraya / Cai, Zexi / Nielsen, Mette Olaf

    Physiological genomics

    2023  Volume 55, Issue 9, Page(s) 392–413

    Abstract: We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target ... ...

    Abstract We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target followed by subcutaneous (SUB) adipose tissue. We aimed to identify coexpressed and hub genes in SUB and PER to identify the underlying molecular mechanisms contributing to the early nutritional programming of adipose-related phenotypic outcomes. Transcriptomes of SUB and PER of male and female adult sheep with different pre- and early postnatal nutrition histories were used to construct networks of coexpressed genes likely to be functionally associated with pre- and early postnatal nutrition histories and phenotypic traits using weighted gene coexpression network analysis. The modules from PER showed enrichment of cell cycle regulation, gene expression, transmembrane transport, and metabolic processes associated with both sexes' prenatal nutrition. In SUB (only males), a module of enriched adenosine diphosphate metabolism and development correlated with prenatal nutrition. Sex-specific module enrichments were found in PER, such as chromatin modification in the male network but histone modification and mitochondria- and oxidative phosphorylation-related functions in the female network. These sex-specific modules correlated with prenatal nutrition and adipocyte size distribution patterns. Our results point to PER as a primary target of prenatal malnutrition compared to SUB, which played only a minor role. The prenatal programming of gene expression and cell cycle, potentially through epigenetic modifications, might be underlying mechanisms responsible for observed changes in PER expandability and adipocyte-size distribution patterns in adulthood in both sexes.
    MeSH term(s) Pregnancy ; Sheep ; Male ; Female ; Animals ; Adipose Tissue/metabolism ; Obesity/genetics ; Malnutrition/genetics ; Malnutrition/metabolism ; Subcutaneous Fat/metabolism ; Adiposity
    Language English
    Publishing date 2023-07-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2038823-8
    ISSN 1531-2267 ; 1094-8341
    ISSN (online) 1531-2267
    ISSN 1094-8341
    DOI 10.1152/physiolgenomics.00128.2022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5697: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Enteric methane emission of dairy cows supplemented with iodoform in a dose-response study.

    Thorsteinsson, Mirka / Lund, Peter / Weisbjerg, Martin Riis / Noel, Samantha Joan / Schönherz, Anna Amanda / Hellwing, Anne Louise Frydendahl / Hansen, Hanne Helene / Nielsen, Mette Olaf

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 12797

    Abstract: Enteric methane ( ... ...

    Abstract Enteric methane (CH
    MeSH term(s) Female ; Cattle ; Animals ; Lactation/physiology ; Diet/veterinary ; Methane/metabolism ; Dietary Supplements/analysis ; Milk/chemistry ; Rumen/metabolism ; Fermentation ; Digestion ; Silage/analysis
    Chemical Substances iodoform (KXI2J76489) ; Methane (OP0UW79H66)
    Language English
    Publishing date 2023-08-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-38149-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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