LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 72

Search options

  1. Article: An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs.

    Xie, Long / Huang, Zhenwen / Lan, Meiyu / Cao, Yaqi / Sun, Lingling / Zhang, Lang / Zuo, Erwei / Lu, Yangqing

    Journal of biological engineering

    2024  Volume 18, Issue 1, Page(s) 5

    Abstract: Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, we used ...

    Abstract Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, we used site-specific integration of an EGFP fused to the OVAL gene by a rigid linker, (EAAAK)
    Language English
    Publishing date 2024-01-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2391582-1
    ISSN 1754-1611
    ISSN 1754-1611
    DOI 10.1186/s13036-023-00390-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Chromosome-level genome of Ambrosia trifida provides insights into adaptation and the evolution of pollen allergens.

    Yin, Lijuan / Zhang, Guangzhong / Zhou, Chikai / Ou, Zhenghui / Qu, Bo / Zhao, Haoyu / Zuo, Erwei / Liu, Bo / Wan, Fanghao / Qian, Wanqiang

    International journal of biological macromolecules

    2024  Volume 259, Issue Pt 2, Page(s) 129232

    Abstract: Ambrosia trifida (giant ragweed) is an invasive plant that can cause serious damage to natural ecosystems and severe respiratory allergies. However, the genomic basis of invasive adaptation and pollen allergens in Ambrosia species remain largely unknown. ...

    Abstract Ambrosia trifida (giant ragweed) is an invasive plant that can cause serious damage to natural ecosystems and severe respiratory allergies. However, the genomic basis of invasive adaptation and pollen allergens in Ambrosia species remain largely unknown. Here, we present a 1.66 Gb chromosome-scale reference genome for giant ragweed and identified multiple types of genome duplications, which are responsible for its rapid environmental adaptation and pollen development. The largest copies number and species-specific expansions of resistance-related gene families compared to Heliantheae alliance might contribute to resist stresses, pathogens and rapid adaptation. To extend the knowledge of evolutionary process of allergic pollen proteins, we predicted 26 and 168 potential pollen allergen candidates for giant ragweed and other Asteraceae plant species by combining machine learning and identity screening. Interestingly, we observed a specific tandemly repeated array for potential allergenic pectate lyases among Ambrosia species. Rapid evolutionary rates on putative pectate lyase allergens may imply a crucial role of nonsynonymous mutations on amino acid residues for plant biological function and allergenicity. Altogether, this study provides insight into the molecular ecological adaptation and putative pollen allergens prediction that will be helpful in promoting invasion genomic research and evolution of putative pollen allergy in giant ragweed.
    MeSH term(s) Ambrosia/genetics ; Antigens, Plant/genetics ; Ecosystem ; Allergens/genetics ; Allergens/chemistry ; Pollen/genetics ; Hypersensitivity ; Chromosomes
    Chemical Substances Antigens, Plant ; Allergens
    Language English
    Publishing date 2024-01-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.129232
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs

    Long Xie / Zhenwen Huang / Meiyu Lan / Yaqi Cao / Lingling Sun / Lang Zhang / Erwei Zuo / Yangqing Lu

    Journal of Biological Engineering, Vol 18, Iss 1, Pp 1-

    2024  Volume 9

    Abstract: Abstract Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, ...

    Abstract Abstract Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, we used site-specific integration of an EGFP fused to the OVAL gene by a rigid linker, (EAAAK)3, at the endogenous ovalbumin locus in chicken primordial germ cells to generate OVAL-E3-EGFP transgenic chickens, with transgenic chickens expressing CMV immediate enhancer/β-actin-driven EGFP (CAG-EGFP) as a non-secreted control. In OVAL-E3-EGFP chickens, EGFP protein produced in maternal oviducts accumulates to high levels in eggs, but not in eggs of CAG-EGFP chickens. These results indicated that the secretion of foreign proteins can be substantially increased through fusion to the highly secreted endogenous ovalbumin. This study describes a basis for high yield recombinant protein expression in chicken eggs, enabling rapid and scalable production of numerous pharmaceutical proteins or metabolites.
    Keywords Genetically modified chicken ; Deposited foreign protein in eggs ; Recombinant protein secretion ; Nonsecretory protein secretion ; Bioreactor ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Undetectable off-target effects induced by FokI catalytic domain in mouse embryos.

    Xie, Long / Feng, Hu / Li, Zhifang / Li, Di / Yang, Xiali / Yuan, Tanglong / Yan, Nana / He, Chenfei / Zheng, Jitan / Zuo, Zhenrui / Zheng, Yaxuan / Cao, Yaqi / Lu, Yangqing / Xiong, Xing Yao / Zuo, Erwei

    Genome biology

    2024  Volume 25, Issue 1, Page(s) 51

    Abstract: The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target effects is essential for developing these tools. We use Genome-wide Off-target analysis by Two- ...

    Abstract The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target effects is essential for developing these tools. We use Genome-wide Off-target analysis by Two-cell embryo Injection (GOTI) to detect low-frequency off-target editing events in mouse embryos injected with FokI-based architectures. Specifically, we test FokI-heterodimers fused with TALENs, FokI homodimers fused with RYdCas9, or FokI catalytic domains alone resulting in no significant off-target effects. These FokI genome editing systems exhibit undetectable off-target effects in mouse embryos, supporting the further development of these systems for clinical applications.
    MeSH term(s) Animals ; Mice ; Catalytic Domain ; Gene Editing/methods ; Genome ; CRISPR-Cas Systems
    Language English
    Publishing date 2024-02-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2040529-7
    ISSN 1474-760X ; 1474-760X
    ISSN (online) 1474-760X
    ISSN 1474-760X
    DOI 10.1186/s13059-024-03188-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Base editing technology

    Yidi SUN, Erwei ZUO, Hui YANG

    Frontiers of Agricultural Science and Engineering, Vol 7, Iss 2, Pp 227-

    2020  Volume 228

    Keywords Agriculture (General) ; S1-972
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher Higher Education Press
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Treatment of autosomal dominant retinitis pigmentosa caused by RHO-P23H mutation with high-fidelity Cas13X in mice.

    Yan, Zixiang / Yao, Yuqin / Li, Luyao / Cai, Lingqiong / Zhang, Haiwei / Zhang, Shenghai / Xiao, Qingquan / Wang, Xing / Zuo, Erwei / Xu, Chunlong / Wu, Jihong / Yang, Hui

    Molecular therapy. Nucleic acids

    2023  Volume 33, Page(s) 750–761

    Abstract: Mutations ... ...

    Abstract Mutations in
    Language English
    Publishing date 2023-08-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2662631-7
    ISSN 2162-2531
    ISSN 2162-2531
    DOI 10.1016/j.omtn.2023.08.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: Human cleaving embryos enable efficient mitochondrial base-editing with DdCBE.

    Wei, Yinghui / Xu, Chunlong / Feng, Hu / Xu, Kui / Li, Zhifang / Hu, Jing / Zhou, Ling / Wei, Yu / Zuo, Zhenrui / Zuo, Erwei / Li, Wen / Yang, Hui / Zhang, Meiling

    Cell discovery

    2022  Volume 8, Issue 1, Page(s) 7

    Language English
    Publishing date 2022-02-01
    Publishing country England
    Document type Letter
    ISSN 2056-5968
    ISSN 2056-5968
    DOI 10.1038/s41421-021-00372-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: Mitochondrial base editor DdCBE causes substantial DNA off-target editing in nuclear genome of embryos.

    Wei, Yinghui / Li, Zhifang / Xu, Kui / Feng, Hu / Xie, Long / Li, Di / Zuo, Zhenrui / Zhang, Meiling / Xu, Chunlong / Yang, Hui / Zuo, Erwei

    Cell discovery

    2022  Volume 8, Issue 1, Page(s) 27

    Language English
    Publishing date 2022-03-18
    Publishing country England
    Document type Letter
    ISSN 2056-5968
    ISSN 2056-5968
    DOI 10.1038/s41421-022-00391-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice.

    Yan, Nana / Feng, Hu / Sun, Yongsen / Xin, Ying / Zhang, Haihang / Lu, Hongjiang / Zheng, Jitan / He, Chenfei / Zuo, Zhenrui / Yuan, Tanglong / Li, Nana / Xie, Long / Wei, Wu / Sun, Yidi / Zuo, Erwei

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 1784

    Abstract: Base editors have been reported to induce off-target mutations in cultured cells, mouse embryos and rice, but their long-term effects in vivo remain unknown. Here, we develop a Systematic evaluation Approach For gene Editing tools by Transgenic mIce ( ... ...

    Abstract Base editors have been reported to induce off-target mutations in cultured cells, mouse embryos and rice, but their long-term effects in vivo remain unknown. Here, we develop a Systematic evaluation Approach For gene Editing tools by Transgenic mIce (SAFETI), and evaluate the off-target effects of BE3, high fidelity version of CBE (YE1-BE3-FNLS) and ABE (ABE7.10
    MeSH term(s) Animals ; Mice ; Mice, Transgenic ; Cytosine/metabolism ; Mutation ; Gene Editing/methods ; RNA/genetics ; CRISPR-Cas Systems
    Chemical Substances Cytosine (8J337D1HZY) ; RNA (63231-63-0)
    Language English
    Publishing date 2023-03-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37508-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice

    Nana Yan / Hu Feng / Yongsen Sun / Ying Xin / Haihang Zhang / Hongjiang Lu / Jitan Zheng / Chenfei He / Zhenrui Zuo / Tanglong Yuan / Nana Li / Long Xie / Wu Wei / Yidi Sun / Erwei Zuo

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Volume 12

    Abstract: The potential off-target effects of long-term expression of base editors in vivo are unclear. Here the authors report SAFETI, Systematic evaluation Approach For gene Editing tools by Transgenic mIce, to examine off-target effects of base editors over ... ...

    Abstract The potential off-target effects of long-term expression of base editors in vivo are unclear. Here the authors report SAFETI, Systematic evaluation Approach For gene Editing tools by Transgenic mIce, to examine off-target effects of base editors over time in mice, and see abnormal side effects.
    Keywords Science ; Q
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top