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  1. Article ; Online: Molecular detection of

    Bacelar, Polyanna A A / Jaeger, Lauren H / Calegar, Deiviane A / Santos, Jéssica P / Coronato-Nunes, Beatriz / Reis, Elis R C / Bóia, Márcio N / Monteiro, Kerla J L / Carvalho-Costa, Filipe A

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2022  Volume 34, Issue 4, Page(s) 689–692

    Abstract: Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the ... ...

    Abstract Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus
    MeSH term(s) Animals ; Brazil/epidemiology ; Metastrongyloidea/genetics ; Species Specificity ; Strongylida Infections/epidemiology ; Strongylida Infections/parasitology ; Strongylida Infections/veterinary ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/epidemiology ; Swine Diseases/parasitology
    Language English
    Publishing date 2022-05-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387221092143
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Infections with

    Calegar, Deiviane A / Monteiro, Kerla J L / Gonçalves, Andressa B / Boia, Márcio N / Jaeger, Lauren H / Nunes, Beatriz C / Carvalho-Costa, Filipe A

    Journal of tropical medicine

    2020  Volume 2020, Page(s) 3134849

    Abstract: This study aims to assess the prevalence, distribution, and etiological profile of intestinal parasitism in children living in periurban areas in Cachoeiras de Macacu, Rio de Janeiro, Brazil. A community-based cross-sectional survey ( ...

    Abstract This study aims to assess the prevalence, distribution, and etiological profile of intestinal parasitism in children living in periurban areas in Cachoeiras de Macacu, Rio de Janeiro, Brazil. A community-based cross-sectional survey (
    Language English
    Publishing date 2020-07-14
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 2546526-0
    ISSN 1687-9694 ; 1687-9686
    ISSN (online) 1687-9694
    ISSN 1687-9686
    DOI 10.1155/2020/3134849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A molecular and morphological study of Ascaris suum in a human-pig contact scenario in northeastern Brazil.

    Bacelar, Polyanna Araújo Alves / Santos, Jéssica Pereira Dos / Calegar, Deiviane Aparecida / Silva, Denilson de Araújo E / Leal, Daniella Nobre / Evangelista, Brenda Bulsara Costa / Reis, Elis Regina Chaves Dos / Mallet, Jacenir Reis Dos Santos / Carvalho-Costa, Filipe Anibal / Jaeger, Lauren Hubert / Monteiro, Kerla Joeline Lima

    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria

    2023  Volume 32, Issue 3, Page(s) e005623

    Abstract: The aim of the present study was to assess morphologic and genetic data on ascariasis in swine (Sus scrofa domesticus) and humans in low-resource rural and periurban communities in the state of Piauí, Brazil. Our cross-sectional survey included 100 fecal ...

    Abstract The aim of the present study was to assess morphologic and genetic data on ascariasis in swine (Sus scrofa domesticus) and humans in low-resource rural and periurban communities in the state of Piauí, Brazil. Our cross-sectional survey included 100 fecal samples obtained from swine and 682 samples from humans. Fifteen pigs were necropsied. Human and porcine fecal samples were examined to identify Ascaris eggs. Parasites obtained in the swine necropsies were studied using scanning electron microscopy (SEM), and the mitochondrial gene encoding the cytochrome oxidase 1 (cox1) enzyme was partially amplified and sequenced for molecular taxonomy and phylogenetic analyses. The overall prevalence of Ascaris eggs in the swine fecal samples was 16/100 (16%). No Ascaris eggs were identified in the human fecal samples. SEM of six worms recovered from pigs demonstrated morphological characteristics of A. suum. Cox1 sequences were compatible with A. suum reference sequences. Original and reference (GenBank) nucleotide sequences were organized into clusters that did not segregate the parasites by host species or and region. The largest haplogroups were dominated by haplotypes H01, H02 and H31. In the communities studied, there was no epidemiological evidence of the zoonotic transmission of ascariasis at the human-swine interface.
    MeSH term(s) Humans ; Animals ; Swine ; Ascaris suum/genetics ; Ascariasis/epidemiology ; Ascariasis/veterinary ; Ascariasis/parasitology ; Phylogeny ; Brazil ; Cross-Sectional Studies ; Ascaris/genetics ; Swine Diseases/epidemiology ; Swine Diseases/parasitology
    Language English
    Publishing date 2023-10-13
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2578912-0
    ISSN 1984-2961 ; 0103-846X
    ISSN (online) 1984-2961
    ISSN 0103-846X
    DOI 10.1590/S1984-29612023057
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Status of benzimidazole resistance in intestinal nematode populations of livestock in Brazil: a systematic review.

    Jaeger, Lauren Hubert / Carvalho-Costa, Filipe Anibal

    BMC veterinary research

    2017  Volume 13, Issue 1, Page(s) 358

    Abstract: Background: Benzimidazoles (BZ) are a class of drugs widely used in veterinary and human medicine, creating a great selection pressure and the emergence of BZ resistance. We conducted a systematic review to assess the status of resistance and/or ... ...

    Abstract Background: Benzimidazoles (BZ) are a class of drugs widely used in veterinary and human medicine, creating a great selection pressure and the emergence of BZ resistance. We conducted a systematic review to assess the status of resistance and/or effectiveness reduction of BZ drugs in animal nematodes in Brazil, and make information accessible to the scientific community, as many studies are published in Portuguese. PubMed, SciELO Brasil, LILACS/Bireme, GNTD database, and Google Scholar were searched with no language restrictions.
    Results: A total of 40 studies met our eligibility criteria (from the year 1989 forward). Sheep was the host most frequently analysed, and albendazole was the most frequently drug studied. The majority of studies (75.7%) showed that BZ drugs are insufficiently active (FECRT <80%) against nematode parasites of livestock. The mean FECRT for fenbendazole, thiabendazole, albendazole, mebendazole, oxfendazole, and ricobendazole were 71.8%, 71.8%, 58.6%, 53.9%, 46.9%, and 41.5%, respectively. It was observed through linear regression that FECRT is significantly reduced over time between 2007 and 2014 (R = -0.653 p = 0.021) for the treatment of cattle with BZ, suggesting progressive loss of effectiveness and increased resistance for these hosts.
    Conclusions: The scenario of BZ resistance in nematode populations in Brazil is not favourable. Given the high cost of drug discovery and development, it is urgent to implement control measures and to monitor the effectiveness/resistance to nematodes in livestock in Brazil.
    MeSH term(s) Animals ; Antinematodal Agents/pharmacology ; Benzimidazoles/pharmacology ; Brazil ; Drug Resistance ; Livestock/parasitology ; Nematoda/drug effects ; Nematode Infections/drug therapy ; Nematode Infections/parasitology ; Nematode Infections/veterinary ; Parasite Egg Count/veterinary
    Chemical Substances Antinematodal Agents ; Benzimidazoles
    Language English
    Publishing date 2017-11-25
    Publishing country England
    Document type Journal Article ; Review ; Systematic Review
    ISSN 1746-6148
    ISSN (online) 1746-6148
    DOI 10.1186/s12917-017-1282-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions

    Calegar, Deiviane A / Nunes, Beatriz C / Monteiro, Kerla J L / Bacelar, Polyanna A A / Evangelista, Brenda B C / Almeida, Mayron M / Silva, Jurecir / Santos, Jéssica P / Boia, Márcio N / Jaeger, Lauren H / Carvalho-Costa, Filipe A

    Microorganisms

    2022  Volume 10, Issue 5

    Abstract: Human infections with gut protozoan parasites are neglected and not targeted by specific control initiatives, leading to a knowledge gap concerning their regional diversity and epidemiology. The present study aims to explore Giardia duodenalis genetic ... ...

    Abstract Human infections with gut protozoan parasites are neglected and not targeted by specific control initiatives, leading to a knowledge gap concerning their regional diversity and epidemiology. The present study aims to explore Giardia duodenalis genetic diversity and assess the epidemiologic scenario of subclinical infections in different Brazilian biogeographic regions. Cross-sectional surveys (n = 1334 subjects) were conducted in four municipalities in order to obtain fecal samples and socioenvironmental data. Microscopy of non-diarrheal feces and nucleotide sequencing of a β-giardin gene fragment were performed. From a total of 51 samples that could be sequenced, 27 (52.9%) β-giardin sequences were characterized as assemblage A and 24 (47.1%) as assemblage B. In the Amazon, assemblage B was the most frequently detected, predominantly BIII, and with two novel sub-assemblages. Assemblage A predominated in the extra-Amazon region, with five novel sub-assemblages. Prevalence reached 17.8% (64/360) in the Amazon, 8.8% (48/544) in the Atlantic Forest, 7.4% (22/299) in Cerrado and 2.3% (3/131) in the Semiarid. People living in poverty and extreme poverty presented significantly higher positivity rates. In conclusion, subclinical giardiasis is endemic in Brazilian communities in different biogeographic regions, presenting high genetic diversity and a heterogeneous genotypic distribution.
    Language English
    Publishing date 2022-04-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10050940
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Cytochrome c oxidase subunit 1 gene reveals species composition and phylogenetic relationships of Oesophagostomum spp. infecting pigs in northeastern Brazil.

    Bacelar, Polyanna Araújo Alves / Monteiro, Kerla Joeline Lima / Calegar, Deiviane Aparecida / Santos, Jéssica Pereira Dos / Coronato-Nunes, Beatriz / Reis, Elis Regina Chaves Dos / Bóia, Márcio Neves / Jaeger, Lauren Hubert / Carvalho-Costa, Filipe Anibal

    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria

    2022  Volume 31, Issue 1, Page(s) e018421

    Abstract: Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans ...

    Abstract Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piauí, Brazil. Eighty-seven fecal samples were collected for parasitological tests and molecular analysis. Through microscopy, the overall positivity rate for strongyliform eggs was 81.6% among the pigs studied. Forty-two strongyliform egg samples were subjected to PCR and six cox1 sequences (637 bp) were identified for the genus Oesophagostomum. The sequences were identified as Oesophagostomum dentatum, O. quadrispinulatum and O. columbianum. In the phylogenetic tree and haplotype network, 89 sequences were separated into seven clusters, which also included reference sequences from GenBank. Oesophagostomum dentatum and O. quadrispinulatum were seen to be closely related species and formed a monophyletic group related to O. aculeatum. Oesophagostomum columbianum showed similarity with sequences from parasites infecting small ruminants and the clade was positioned closer to O. bifurcum. High interspecific diversity was found and intraspecific diversity varied according to the species. This was the first study to characterize Oesophagostomum DNA sequences obtained from pigs in Brazil.
    MeSH term(s) Animals ; Brazil ; Electron Transport Complex IV/genetics ; Oesophagostomum/genetics ; Phylogeny ; Swine ; Swine Diseases/epidemiology ; Swine Diseases/parasitology
    Chemical Substances Electron Transport Complex IV (EC 1.9.3.1)
    Language English
    Publishing date 2022-04-01
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2578912-0
    ISSN 1984-2961 ; 0103-846X
    ISSN (online) 1984-2961
    ISSN 0103-846X
    DOI 10.1590/S1984-29612022016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Status of benzimidazole resistance in intestinal nematode populations of livestock in Brazil: a systematic review

    Jaeger, Lauren Hubert / Carvalho-Costa, Filipe Anibal

    BMC veterinary research. 2017 Dec., v. 13, no. 1

    2017  

    Abstract: BACKGROUND: Benzimidazoles (BZ) are a class of drugs widely used in veterinary and human medicine, creating a great selection pressure and the emergence of BZ resistance. We conducted a systematic review to assess the status of resistance and/or ... ...

    Abstract BACKGROUND: Benzimidazoles (BZ) are a class of drugs widely used in veterinary and human medicine, creating a great selection pressure and the emergence of BZ resistance. We conducted a systematic review to assess the status of resistance and/or effectiveness reduction of BZ drugs in animal nematodes in Brazil, and make information accessible to the scientific community, as many studies are published in Portuguese. PubMed, SciELO Brasil, LILACS/Bireme, GNTD database, and Google Scholar were searched with no language restrictions. RESULTS: A total of 40 studies met our eligibility criteria (from the year 1989 forward). Sheep was the host most frequently analysed, and albendazole was the most frequently drug studied. The majority of studies (75.7%) showed that BZ drugs are insufficiently active (FECRT <80%) against nematode parasites of livestock. The mean FECRT for fenbendazole, thiabendazole, albendazole, mebendazole, oxfendazole, and ricobendazole were 71.8%, 71.8%, 58.6%, 53.9%, 46.9%, and 41.5%, respectively. It was observed through linear regression that FECRT is significantly reduced over time between 2007 and 2014 (R = −0.653 p = 0.021) for the treatment of cattle with BZ, suggesting progressive loss of effectiveness and increased resistance for these hosts. CONCLUSIONS: The scenario of BZ resistance in nematode populations in Brazil is not favourable. Given the high cost of drug discovery and development, it is urgent to implement control measures and to monitor the effectiveness/resistance to nematodes in livestock in Brazil.
    Keywords albendazole ; benzimidazole ; cattle ; cattle diseases ; control methods ; databases ; drug resistance ; drugs ; fenbendazole ; hosts ; mebendazole ; medicine ; Nematoda ; nematode infections ; oxfendazole ; parasites ; regression analysis ; selection pressure ; systematic review ; thiabendazole ; veterinary medicine ; Brazil
    Language English
    Dates of publication 2017-12
    Size p. 358.
    Publishing place BioMed Central
    Document type Article
    ISSN 1746-6148
    DOI 10.1186/s12917-017-1282-2
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.

    Jaeger, Lauren Hubert / Pestana, Cristiane Pinheiro / Carvalho-Costa, Filipe Anibal / Medeiros, Marco Alberto / Lilenbaum, Walter

    Journal of medical microbiology

    2018  Volume 67, Issue 9, Page(s) 1361–1367

    Abstract: Purpose: Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in ... ...

    Abstract Purpose: Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic similarity among the strains of these serovars is known. However, it has not yet been demonstrated whether major clonal subpopulation in humans, strain Fiocruz L1-130-like, can circulate among other hosts.
    Methodology: We performed genetic characterization of Brazilian serogroup Icterohaemorrhagiae strains of dog and rat origin by secY sequencing, variable-number tandem-repeat, multilocus sequence type and multi-spacer typing analysis.
    Results: The strains were found to be identical among themselves and to strain Fiocruz L1-130. We suggest that the major strain of L. interrogans serogroup Icterohaemorrhagiae, Fiocruz L1-130, is widely distributed in Brazil in different hosts with substantial zoonotic potential.
    Conclusion: Understanding the circulation of strain Fiocruz L1-130 is important for the implementation of appropriate control measures. Its circulation highlights the need to treat leptospirosis caused by L. interrogans serogroup Icterohaemorrhagiae as a zoonosis that acts in the human-animal-environment interface, as per the One Health approach.
    MeSH term(s) Animals ; Brazil ; Dog Diseases/microbiology ; Dogs ; Leptospira interrogans/classification ; Leptospira interrogans/genetics ; Leptospira interrogans/isolation & purification ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Minisatellite Repeats ; Multilocus Sequence Typing ; Phylogeny ; Rats ; Rodent Diseases/microbiology
    Language English
    Publishing date 2018-07-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.000806
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Molecular detection of Metastrongylus salmi eggs from pigs in low-resource communities in the state of Piauí, northeastern Brazil

    Bacelar, Polyanna A. A. / Jaeger, Lauren H. / Calegar, Deiviane A. / Santos, Jéssica P. / Coronato-Nunes, Beatriz / Reis, Elis R. C. / Bóia, Márcio N. / Monteiro, Kerla J. L. / Carvalho-Costa, Filipe A.

    Journal of veterinary diagnostic investigation. 2022 July, v. 34, no. 4

    2022  

    Abstract: Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus, whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. ...

    Abstract Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus, whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of Metastrongylus infecting pigs in close contact with humans in impoverished rural communities in the state of Piauí, in northeastern Brazil. Fecal samples (n = 78) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 (cox1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with Metastrongylus. Of the 37 samples submitted to PCR, 10 were identified as M. salmi. We found 3 M. salmi haplotypes, including 2 new and 1 described previously in Europe. Overall, M. salmi demonstrated lower intraspecific genetic diversity: diversity index (H) ± SD = 0.318 ± 0.164, n = 12, compared with published M. pudendotectus sequences (1.000 ± 0.272, n = 3). To our knowledge, M. salmi DNA sequences have not been published previously from pigs in South America.
    Keywords DNA ; Metastrongylus ; Strongylida ; eggs ; genes ; genetic variation ; haplotypes ; light microscopy ; morphometry ; respiratory system ; species diversity ; Brazil ; Europe
    Language English
    Dates of publication 2022-07
    Size p. 689-692.
    Publishing place SAGE Publications
    Document type Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387221092143
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions

    Deiviane A. Calegar / Beatriz C. Nunes / Kerla J. L. Monteiro / Polyanna A. A. Bacelar / Brenda B. C. Evangelista / Mayron M. Almeida / Jurecir Silva / Jéssica P. Santos / Márcio N. Boia / Lauren H. Jaeger / Filipe A. Carvalho-Costa

    Microorganisms, Vol 10, Iss 940, p

    2022  Volume 940

    Abstract: Human infections with gut protozoan parasites are neglected and not targeted by specific control initiatives, leading to a knowledge gap concerning their regional diversity and epidemiology. The present study aims to explore Giardia duodenalis genetic ... ...

    Abstract Human infections with gut protozoan parasites are neglected and not targeted by specific control initiatives, leading to a knowledge gap concerning their regional diversity and epidemiology. The present study aims to explore Giardia duodenalis genetic diversity and assess the epidemiologic scenario of subclinical infections in different Brazilian biogeographic regions. Cross-sectional surveys ( n = 1334 subjects) were conducted in four municipalities in order to obtain fecal samples and socioenvironmental data. Microscopy of non-diarrheal feces and nucleotide sequencing of a β-giardin gene fragment were performed. From a total of 51 samples that could be sequenced, 27 (52.9%) β-giardin sequences were characterized as assemblage A and 24 (47.1%) as assemblage B. In the Amazon, assemblage B was the most frequently detected, predominantly BIII, and with two novel sub-assemblages. Assemblage A predominated in the extra-Amazon region, with five novel sub-assemblages. Prevalence reached 17.8% (64/360) in the Amazon, 8.8% (48/544) in the Atlantic Forest, 7.4% (22/299) in Cerrado and 2.3% (3/131) in the Semiarid. People living in poverty and extreme poverty presented significantly higher positivity rates. In conclusion, subclinical giardiasis is endemic in Brazilian communities in different biogeographic regions, presenting high genetic diversity and a heterogeneous genotypic distribution.
    Keywords Giardia duodenalis ; assemblages ; epidemiology ; genetic diversity ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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