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  1. Article ; Online: Topical cream containing live lactobacilli decreases malodor-producing bacteria and downregulates genes encoding PLP-dependent enzymes on the axillary skin microbiome of healthy adult Nigerians.

    Onwuliri, Victoria / Agbakoba, Nneka R / Anukam, Kingsley C

    Journal of cosmetic dermatology

    2021  Volume 20, Issue 9, Page(s) 2989–2998

    Abstract: Background: Clinical data exist that support the utility of topical probiotics for certain dermatological diseases such as atopic dermatitis, acne, and psoriasis. However, there is paucity of data on the use of live lactobacilli to control axillary ... ...

    Abstract Background: Clinical data exist that support the utility of topical probiotics for certain dermatological diseases such as atopic dermatitis, acne, and psoriasis. However, there is paucity of data on the use of live lactobacilli to control axillary malodor. The objective of this study was to determine whether application of topical oil-based cream containing live Lactobacilli could decrease malodor-producing bacteria in the axilla of healthy subjects.
    Aims: To determine the effects of topical cream with live lactobacilli on malodor-producing bacteria in the axilla of healthy subjects.
    Patients/methods: Twenty-five adult volunteers comprising 12 males and 13 females provided informed consent. Axillary skin swabs were collected before and after 14 days application of topical cream containing live Lactobacillus pentosus KCA1. Bacterial DNA was extracted, and V4 region of the 16S rRNA was amplified and sequenced in a pair-end configuration on the Illumina MiSeq platform rendering 2 × 150 bp sequences. Microbial taxonomy to species level was generated using the Greengenes database. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biologically and statistically significant differences in relative abundance.
    Results: Actinobacteria decreased from 70% to 24%, and Firmicutes increased from 26.6% to 73.9% among the female participants. In males, Actinobacteria decreased from 65% to 38%, while Firmicutes increased from 24% to 57%. Corynebacterium decreased from 62.91% to 36.63%, while Lactobacillus increased from 0.06% to 23.11%. In males, unliked females, there were reduction of Staphylococcus species associated with malodor, notably Staphylococcus hominis, Staphylococcus hemolyticus, and Staphylococcus lugdunensis. Bacterial functional gene- Pyridoxal protein dependent enzymes involved in biotransformation of malodor precursor to volatile thioalcohols were down-regulated.
    Conclusions: Application of Lactobacillus pentosus KCA1 cream led to a significant decrease in the relative abundance of odor-producing Corynebacterium species in both female and male subjects. Some species associated with malodor especially Corynebacterium striatum, Corynebacterium jeikeium, Corynebacterium tuberculostearicum, and Staphylococcus hominis decreased by 96%, 73%, 7%, and 20.8%, respectively, in males.
    MeSH term(s) Adult ; Axilla ; Bacteria/genetics ; Corynebacterium ; Female ; Humans ; Lactobacillus/genetics ; Male ; Microbiota ; Nigeria ; RNA, Ribosomal, 16S
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2021-01-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2280551-5
    ISSN 1473-2165 ; 1473-2130
    ISSN (online) 1473-2165
    ISSN 1473-2130
    DOI 10.1111/jocd.13949
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  2. Article ; Online: Microbiome Compositions From Infertile Couples Seeking

    Okwelogu, Somadina I / Ikechebelu, Joseph I / Agbakoba, Nneka R / Anukam, Kingsley C

    Frontiers in cellular and infection microbiology

    2021  Volume 11, Page(s) 709372

    Abstract: Background: Bacterial infections are usually suspected in infertile couples seeking IVF with no clear understanding of the microbial compositions present in the seminal fluids and vaginal niche of the patients. We used next-generation sequencing ... ...

    Abstract Background: Bacterial infections are usually suspected in infertile couples seeking IVF with no clear understanding of the microbial compositions present in the seminal fluids and vaginal niche of the patients. We used next-generation sequencing technology to correlate microbiota compositions with IVF clinical outcomes.
    Methods: Thirty-six couples were recruited to provide seminal fluids and vaginal swabs. Bacterial DNA was extracted, and V4 region of the 16S rRNA was amplified and sequenced in a pair-end configuration on the Illumina MiSeq platform rendering 2 × 150 bp sequences. Microbial taxonomy to species level was generated using the Greengenes database. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biologically and statistically significant differences in relative abundance.
    Results: Seminal fluid microbiota compositions had lower bacterial concentrations compared with the vagina, but species diversity was significantly higher in seminal fluid samples. Azoospermic subjects had more relative abundance of
    Conclusions: Semen samples with positive IVF were significantly colonized by
    MeSH term(s) Female ; Fertilization in Vitro ; Genes, rRNA ; Humans ; Infertility/microbiology ; Lactobacillus ; Male ; Microbiota ; RNA, Ribosomal, 16S/genetics ; Vagina
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2021-10-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2021.709372
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Bioinformatic analysis of dihydrofolate reductase predicted in the genome sequence of Lactobacillus pentosus KCA1.

    Anukam, Kingsley C / Oge, Uche

    Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria

    2014  Volume 29, Issue 1, Page(s) 47–53

    Abstract: Physiologic studies of Lactobacillus species show that some species cannot synthesize folate de novo, which is required for growth. Folate plays a critical role in regulating the amount of tetrahydrofolate in the cell that is utilized for DNA replication, ...

    Abstract Physiologic studies of Lactobacillus species show that some species cannot synthesize folate de novo, which is required for growth. Folate plays a critical role in regulating the amount of tetrahydrofolate in the cell that is utilized for DNA replication, and proliferation of the erythropoietic system. We recently sequenced the genome of Lactobacillus pentosus KCA1, isolated from a Nigerian subject. The genome has open reading frames coding for the complete genes required for folate biosynthesis. Our previous study shows that rats fed with L. pentosus KCA1 led to enhancement of haematological parameters. Bioinformatic tool such as ClustalW algorithm was used to analyze dihydrofolate reductase (folA/dfrA) encoded in the genome sequence of L. pentosus KCA1 for comparative multiple sequence alignments. I-TASSER was used to predict the 3-D model structure of the protein and potential active binding site residues. Result show that two unique amino acid substitutions were found in KCA1_1610 sequence at position 85 with alanine (A-Ala85), while other strains have aspartic acid (D-Asp) for other L. pentosus and threonine (T-Thr) for L. plantarum strains at the same position. The result suggests that dihydrofolate reductase can be used as a distinguishing marker between L. pentosus KCA1 and other pentosus including L. plantarum strains. The secondary structure prediction with I-TASSER revealed 5 alpha helices and 8 beta-strands. Twelve binding site residues were predicted in KCA1_1610 relative to the template protein 2zzaA in protein database (PDB). The predicted structure of KCA1_1610 dihydrofolate reductase can serve as a new template as an addition to structural genomics and generation of models for use in drug screening and physiological function inference.
    MeSH term(s) Amino Acid Sequence ; Computational Biology/methods ; DNA, Bacterial/genetics ; Forecasting ; Genome, Bacterial/genetics ; Humans ; Lactobacillus pentosus/chemistry ; Lactobacillus pentosus/enzymology ; Lactobacillus pentosus/genetics ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Tetrahydrofolate Dehydrogenase/chemistry ; Tetrahydrofolate Dehydrogenase/genetics
    Chemical Substances DNA, Bacterial ; Tetrahydrofolate Dehydrogenase (EC 1.5.1.3)
    Language English
    Publishing date 2014-06-19
    Publishing country Nigeria
    Document type Journal Article
    ZDB-ID 2197951-0
    ISSN 0794-859X
    ISSN 0794-859X
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  4. Article ; Online: African traditional fermented foods and probiotics.

    Anukam, Kingsley C / Reid, Gregor

    Journal of medicinal food

    2009  Volume 12, Issue 6, Page(s) 1177–1184

    Abstract: African traditional fermented foods remain the main source of nutrition for many rural communities in Africa. Although lactic acid bacteria are integral to many of these foods, little is known about the specific health benefits they confer or the ... ...

    Abstract African traditional fermented foods remain the main source of nutrition for many rural communities in Africa. Although lactic acid bacteria are integral to many of these foods, little is known about the specific health benefits they confer or the properties of their strains. This mini-review explores the history of some African fermented foods and their microbial content and properties within the context of probiotic characteristics. Given the recent upsurge in probiotic research, recommendations are made on studies that could be performed with African fermented foods and their strains, with a view to improving the health of people in sub-Saharan Africa.
    MeSH term(s) Africa ; Bacteria/metabolism ; Fermentation ; Food Microbiology ; Food, Organic/analysis ; Food, Organic/microbiology ; Humans ; Probiotics/metabolism
    Language English
    Publishing date 2009-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1427365-2
    ISSN 1557-7600 ; 1096-620X
    ISSN (online) 1557-7600
    ISSN 1096-620X
    DOI 10.1089/jmf.2008.0163
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  5. Article: African Traditional Fermented Foods and Probiotics

    Anukam, Kingsley C / Reid, Gregor

    Journal of medicinal food. 2009 Dec., v. 12, no. 6

    2009  

    Keywords fermented foods ; traditional foods ; probiotics ; lactic acid bacteria ; functional foods ; human health ; Sub-Saharan Africa
    Language English
    Dates of publication 2009-12
    Size p. 1177-1184.
    Document type Article
    ZDB-ID 1427365-2
    ISSN 1096-620X
    ISSN 1096-620X
    DOI 10.1089/jmf.2008.0163
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: The potential role of probiotics in reducing poverty-associated infections in developing countries

    Kingsley C. Anukam

    Journal of Infection in Developing Countries, Vol 1, Iss 2, Pp 81-

    2007  Volume 83

    Abstract: Probiotics are defined by the Food and Agricultural Organization/ World Health Organization as “live microorganisms which when administered in adequate amounts confer a health benefit on the host” [1]. The potential benefits of their use have not been ... ...

    Abstract Probiotics are defined by the Food and Agricultural Organization/ World Health Organization as “live microorganisms which when administered in adequate amounts confer a health benefit on the host” [1]. The potential benefits of their use have not been adequately investigated, especially in the developing world. Japan introduced Yakult, a probiotic fermented food drink in 1935, and in the Northern hemisphere, research and use of probiotics has gained an unprecedented momentum in the last decade [2]. Use of probiotics is not uncommon in Europe [3], but in many developing countries use of probiotics in its present definition is a foreign concept. Some African traditional foods are fermented with lactic acid bacteria, and some may have probiotic properties, but clinical evidence is yet to be deciphered. Nonetheless, many communities rushing to “westernize” are losing their fermented food traditions.
    Keywords Probiotics ; Developing Countries ; Infections ; Internal medicine ; RC31-1245 ; Microbiology ; QR1-502
    Language English
    Publishing date 2007-10-01T00:00:00Z
    Publisher The Journal of Infection in Developing Countries
    Document type Article ; Online
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  7. Article: In vitro evaluation of the viability of vaginal cells (VK2/E6E7) and probiotic Lactobacillus species in lemon juice.

    Anukam, Kingsley C / Reid, Gregor

    Sexual health

    2009  Volume 6, Issue 1, Page(s) 67–74

    Abstract: Background: Women, especially in developing countries, most often bear the brunt of HIV infections. The continued lack of viable vaccines and microbicides has made some women resort to using natural products such as lemon or lime juice to avoid ... ...

    Abstract Background: Women, especially in developing countries, most often bear the brunt of HIV infections. The continued lack of viable vaccines and microbicides has made some women resort to using natural products such as lemon or lime juice to avoid infection. Few in vitro studies have been done on the effect of lemon juice on vaginal cells and lactobacilli that constitute the major microbiota in healthy women.
    Objective: The objective of the present study was to evaluate in vitro the effect of lemon juice on the viability of vaginal cells (VK2/E6E7) and vaginal Lactobacillus species.
    Methods: Vaginal cells were exposed to different concentrations (0-30%) of lemon juice at pH 2.3 and 4.5 for 10 min. Viability was determined by staining the cells with propidium iodide and analysing them by flow cytometry. Lactobacillus organisms were dispensed into microplates with vaginally defined medium + peptone (VDMP) containing different concentrations of lemon juice ranging from 0 to 100%.
    Results: Lemon juice at pH 2.3 had a significant (P = 0.03) toxic effect on the vaginal cell line used. At 30% concentration, the vaginal cells were practically non-viable, typified by a 95% loss of viability, whereas at pH 4.5 there was only 5% cell loss. Lemon juice had varying growth inhibitory effects on the Lactobacillus species tested. At pH 4.5 and using 10-30% lemon juice, there was a stimulatory growth effect on certain Lactobacillus species.
    Conclusions: Lemon juice (20-30%) at pH 2.3 was highly toxic to VK2/E6E7 cells, and at pH 4.5 there was no significant effect on the viability of the cells within 10 min. Lemon juice above 10% at pH 2.3 was found to be detrimental to the growth of vaginal lactobacilli. Although lemon juice may be useful in other applications, its use in the vaginal region should be discouraged.
    MeSH term(s) Anti-Infective Agents, Local/pharmacology ; Beverages ; Cells, Cultured/drug effects ; Citrus aurantiifolia ; Female ; Humans ; Hydrogen-Ion Concentration ; Lactobacillus/drug effects ; Lactobacillus/growth & development ; Species Specificity ; Vagina/cytology ; Vagina/microbiology
    Chemical Substances Anti-Infective Agents, Local
    Language English
    Publishing date 2009-01-20
    Publishing country Australia
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2256731-8
    ISSN 1449-8987 ; 1448-5028
    ISSN (online) 1449-8987
    ISSN 1448-5028
    DOI 10.1071/sh08037
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6421: Show issues Location:
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  8. Article ; Online: Organisms associated with bacterial vaginosis in Nigerian women as determined by PCR-DGGE and 16S rRNA gene sequence.

    Anukam, Kingsley C / Reid, Gregor

    African health sciences

    2007  Volume 7, Issue 2, Page(s) 68–72

    Abstract: Background: Bacterial vaginosis (BV) is a condition with diverse etiology. This condition predisposes women to increased susceptibility to sexually transmitted diseases, including human immunodeficiency virus (HIV) infections and preterm birth. The ... ...

    Abstract Background: Bacterial vaginosis (BV) is a condition with diverse etiology. This condition predisposes women to increased susceptibility to sexually transmitted diseases, including human immunodeficiency virus (HIV) infections and preterm birth. The diagnostic methods currently adopted in the evaluation of patient samples for BV are arguably Amsel criteria, and Nugent score that require microscopy and expert interpretation. These two methods are still subjective.
    Objective: The objective of this study was to determine the organisms present in the vagina of 34 HIV negative Nigerian women diagnosed as having bacterial vaginosis by using molecular techniques.
    Methods: The vaginal samples were subjected to DNA extraction, and amplified with eubacterial primers via PCR. The PCR products were separated using denaturing gradient gel electrophoresis (DGGE). Bands were excised, re-amplified, purified and sequenced. Sequence identification was performed using the BLAST algorithm and Genbank data base.
    Results: Mycoplasma hominis (12/34; 35%) was the most common isolate and 9 (26%) contained one of two clones of an unusual Rainbow Trout intestinal bacterium, while unculturable Streptococcus sp, and other bacteria made up the remaining isolates.
    Conclusions: The findings indicate further diversity in the etiological agents associated with BV, and raise the question as to whether diagnosis and management of this condition needs to be re-evaluated in countries like Nigeria. There is some controversy over the clinical importance of BV, as it was once regarded as a disease caused by Gardnerella and presenting as an odourous discharge condition, but is now diagnosed without necessarily the presence of these organisms or signs. With the incidence of BV aligned to an increased risk of HIV in a country ravaged by this virus, the effective eradication of BV can only be achieved if appropriate therapies are delivered.
    MeSH term(s) DNA, Bacterial/analysis ; Electrophoresis, Agar Gel/methods ; Female ; Humans ; Molecular Sequence Data ; Mycoplasma hominis/pathogenicity ; Nigeria ; Polymerase Chain Reaction/methods ; RNA, Ribosomal, 16S/genetics ; Vaginosis, Bacterial/diagnosis ; Vaginosis, Bacterial/etiology ; Vaginosis, Bacterial/microbiology
    Chemical Substances DNA, Bacterial ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2007-06
    Publishing country Uganda
    Document type Journal Article
    ZDB-ID 2240308-5
    ISSN 1729-0503 ; 1680-6905
    ISSN (online) 1729-0503
    ISSN 1680-6905
    DOI 10.5555/afhs.2007.7.2.68
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    Zs.A 3851: Show issues Location:
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  9. Article: Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 may help downregulate TNF-Alpha, IL-6, IL-8, IL-10 and IL-12 (p70) in the neurogenic bladder of spinal cord injured patient with urinary tract infections: a two-case study.

    Anukam, Kingsley C / Hayes, Keith / Summers, Kelly / Reid, Gregor

    Advances in urology

    2009  , Page(s) 680363

    Abstract: The management of urinary tract infection (UTI) in individuals with spinal cord injury (SCI) continues to be of concern, due to complications that can occur. An emerging concept that is a common underlying pathophysiological process is involved, wherein ... ...

    Abstract The management of urinary tract infection (UTI) in individuals with spinal cord injury (SCI) continues to be of concern, due to complications that can occur. An emerging concept that is a common underlying pathophysiological process is involved, wherein pathogens causing UTI have a role in inflammatory progression. We hypothesized that members of the commensal flora, such as lactobacilli, may counter this reaction through anti-inflammatory mediation. This was assessed in a pilot two-patient study in which probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri were administered to one patient and placebo to another, both along with antibiotics to treat acute UTI. Urinary TNF-alpha was significantly downregulated (P = .015) in the patient who received the probiotic and who used intermittent catheterization compared with patient on placebo and using an indwelling catheter. The extent to which this alteration resulted in improved well-being in spinal cord injured patients remains to be determined in a larger study.
    Language English
    Publishing date 2009-06-04
    Publishing country Egypt
    Document type Case Reports
    ZDB-ID 2397564-7
    ISSN 1687-6377 ; 1687-6369
    ISSN (online) 1687-6377
    ISSN 1687-6369
    DOI 10.1155/2009/680363
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 May Help Downregulate TNF-Alpha, IL-6, IL-8, IL-10 and IL-12 (p70) in the Neurogenic Bladder of Spinal Cord Injured Patient with Urinary Tract Infections

    Gregor Reid / Kelly Summers / Keith Hayes / Kingsley C. Anukam

    Advances in Urology, Vol

    A Two-Case Study

    2009  Volume 2009

    Keywords Diseases of the genitourinary system. Urology ; RC870-923 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Urology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2009-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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