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Article ; Online: Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods.

Ball, Kristen / Kinne, Renee / Aguirre, Aitor

Journal of visualized experiments : JoVE

2020 , Issue 157

Abstract: Congenital heart defects (CHD) are the most common type of birth defect in humans, affecting up to 1% of all live births. However, the underlying causes for CHD are still poorly understood. The developing mouse constitutes a valuable model for the study ... ...

Abstract	Congenital heart defects (CHD) are the most common type of birth defect in humans, affecting up to 1% of all live births. However, the underlying causes for CHD are still poorly understood. The developing mouse constitutes a valuable model for the study of CHD, because cardiac developmental programs between mice and humans are highly conserved. The protocol describes in detail how to produce mouse embryos of the desired gestational stage, methods to isolate and preserve the heart for downstream processing, quantitative methods to identify common types of CHD by histology (e.g., ventricular septal defects, atrial septal defects, patent ductus arteriosus), and quantitative histomorphometry methods to measure common muscular compaction phenotypes. These methods articulate all the steps involved in sample preparation, collection, and analysis, allowing scientists to correctly and reproducibly measure CHD.
MeSH term(s)	Animals ; Embryo, Mammalian/abnormalities ; Embryo, Mammalian/pathology ; Female ; Heart/embryology ; Heart Defects, Congenital/embryology ; Heart Defects, Congenital/pathology ; Histocytochemistry/methods ; Humans ; Mice, Inbred C57BL ; Myocardium/pathology ; Paraffin Embedding ; Phenotype
Language	English
Publishing date	2020-03-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/60926
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Schistosoma indicum, A rarely reported finding in a dromedary - A case report

Schuster, R.K. / Maio, E. / Kinne, J.

Journal of camel practice and research. 2020 Dec. 11, v. 27, no. 3

2020

Abstract: A carcass of an emaciated female dromedary imported from Pakistan to the United Arab Emirates was sent for necropsy. The main pathological alterations were liver amyloidosis and thickening of the stomachal wall (compartment 3). Two pairs of schistosomes ... ...

Abstract	A carcass of an emaciated female dromedary imported from Pakistan to the United Arab Emirates was sent for necropsy. The main pathological alterations were liver amyloidosis and thickening of the stomachal wall (compartment 3). Two pairs of schistosomes were discovered in the mesenteric veins of the small and large intestines. Morphological features matched those of Schistosoma indicum.
Keywords	Camelus dromedarius ; Schistosoma ; amyloidosis ; camels ; case studies ; females ; liver ; necropsy ; research ; Pakistan
Language	English
Dates of publication	2020-1211
Size	p. 285-287.
Publishing place	Camel Publishing House
Document type	Article
ISSN	2277-8934
DOI	10.5958/2277-8934.2020.00038.7
Database	NAL-Catalogue (AGRICOLA)

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Article: The Inverse Spacer-A Novel, Safe, and Cost-Effective Approach in Routine Procedures for Revision Knee Arthroplasty.

Hammerich, Kristoff / Pollack, Jens / Hasse, Alexander F / El Saman, André / Huber, René / Rupp, Markus / Alt, Volker / Kinne, Raimund W / Mika, Joerg

Journal of clinical medicine

2021 Volume 10, Issue 5

Abstract: ... R-TKA) is the risk of (sub-) luxation during mobilization in the prosthesis-free interval, limiting ... stability. This spacer was implanted during an R-TKA in 110 knees with diagnosed or suspected periprosthetic ...

Abstract	Background: A major disadvantage of current spacers for two-stage revision total knee arthroplasty (R-TKA) is the risk of (sub-) luxation during mobilization in the prosthesis-free interval, limiting their clinical success with detrimental consequences for the patient. The present study introduces a novel inverse spacer, which prevents major complications, such as spacer (sub-) luxations and/or fractures of spacer or bone. Methods: The hand-made inverse spacer consisted of convex tibial and concave femoral components of polymethylmethacrylate bone cement and was intra-operatively molded under maximum longitudinal tension in 5° flexion and 5° valgus position. Both components were equipped with a stem for rotational stability. This spacer was implanted during an R-TKA in 110 knees with diagnosed or suspected periprosthetic infection. Postoperative therapy included a straight leg brace and physiotherapist-guided, crutch-supported mobilization with full sole contact. X-rays were taken before and after prosthesis removal and re-implantation. Results: None of the patients experienced (sub-) luxations/fractures of the spacer, periprosthetic fractures, or soft tissue compromise requiring reoperation. All patients were successfully re-implanted after a prosthesis-free interval of 8 weeks, except for three patients requiring an early exchange of the spacer due to persisting infection. In these cases, the prosthetic-free interval was prolonged for one week. Conclusion: The inverse spacer in conjunction with our routine procedure is a safe and cost-effective alternative to other articulating or static spacers, and allows crutch-supported sole contact mobilization without major post-operative complications. Maximum longitudinal intra-operative tension in 5° flexion and 5° valgus position appears crucial for the success of surgery.
Language	English
Publishing date	2021-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm10050971
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Article ; Online: Stromal architecture directs early dissemination in pancreatic ductal adenocarcinoma.

Ray, Arja / Callaway, Mackenzie K / Rodríguez-Merced, Nelson J / Crampton, Alexandra L / Carlson, Marjorie / Emme, Kenneth B / Ensminger, Ethan A / Kinne, Alexander A / Schrope, Jonathan H / Rasmussen, Haley R / Jiang, Hong / DeNardo, David G / Wood, David K / Provenzano, Paolo P

JCI insight

2022 Volume 7, Issue 3

Abstract: Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here, in both murine and human PDA, we demonstrate that extracellular matrix architecture regulates cell extrusion and subsequent invasion from intact ductal structures ...

Abstract	Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here, in both murine and human PDA, we demonstrate that extracellular matrix architecture regulates cell extrusion and subsequent invasion from intact ductal structures through tumor-associated collagen signatures (TACS). This results in early dissemination from histologically premalignant lesions and continual invasion from well-differentiated disease, and it suggests TACS as a biomarker to aid in the pathologic assessment of early disease. Furthermore, we show that pancreatitis results in invasion-conducive architectures, thus priming the stroma prior to malignant disease. Analysis in potentially novel microfluidic-derived microtissues and in vivo demonstrates decreased extrusion and invasion following focal adhesion kinase (FAK) inhibition, consistent with decreased metastasis. Thus, data suggest that targeting FAK or strategies to reengineer and normalize tumor microenvironments may have roles not only in very early disease, but also for limiting continued dissemination from unresectable disease. Likewise, it may be beneficial to employ stroma-targeting strategies to resolve precursor diseases such as pancreatitis in order to remove stromal architectures that increase risk for early dissemination.
MeSH term(s)	Animals ; Apoptosis ; Carcinoma, Pancreatic Ductal/genetics ; Carcinoma, Pancreatic Ductal/pathology ; Carcinoma, Pancreatic Ductal/therapy ; Cell Line, Tumor ; Cell Movement ; Focal Adhesion Kinase 1/antagonists & inhibitors ; Focal Adhesion Kinase 1/biosynthesis ; Focal Adhesion Kinase 1/genetics ; Gene Expression Regulation, Neoplastic ; Humans ; Mice ; Mice, Transgenic ; Neoplasms, Experimental ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/pathology ; Pancreatic Neoplasms/therapy ; RNA, Small Interfering/genetics ; Tumor Microenvironment/genetics
Chemical Substances	RNA, Small Interfering ; Focal Adhesion Kinase 1 (EC 2.7.10.2) ; Ptk2 protein, mouse (EC 2.7.10.2)
Language	English
Publishing date	2022-02-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.150330
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Article ; Online: A re-description of Physocephalus dromedarii stat. nov. (Nematoda: Spirocercidae), an abomasal nematode of dromedaries (Camelus dromedarius).

Schuster, R K / Wibbelt, G / Kinne, J

Journal of helminthology

2014 Volume 88, Issue 4, Page(s) 499–505

Abstract: Physocephalus dromedarii stat. nov. was found in the abomasum of two adult female dromedaries originating from a farm in Dubai. Previously, this nematode has been misidentified as a subspecies of Ph. sexalatus but morphological differences are striking ... ...

Abstract	Physocephalus dromedarii stat. nov. was found in the abomasum of two adult female dromedaries originating from a farm in Dubai. Previously, this nematode has been misidentified as a subspecies of Ph. sexalatus but morphological differences are striking enough for it to be upgraded to a species. Physocephalus dromedarii is larger than Ph. sexalatus and has longer spicules. There are 20-22 oblique crests at the ventral surface between the caudal alae of the male compared to 7-8 in the case of Ph. sexalatus. The most significant differences are the presence of two pairs of papillae between cloaca and postcloacal plate of the male and the existence of a swelling in the second half of the female body formed by loops of both uterine horns.
MeSH term(s)	Abomasum/parasitology ; Animals ; Camelus ; Female ; Male ; Nematoda/classification ; Nematoda/ultrastructure ; Nematode Infections/parasitology ; Nematode Infections/veterinary ; Species Specificity ; Stomach Diseases/parasitology ; Stomach Diseases/veterinary ; United Arab Emirates/epidemiology
Language	English
Publishing date	2014-12
Publishing country	England
Document type	Journal Article
ZDB-ID	390188-9
ISSN	1475-2697 ; 0022-149X
ISSN (online)	1475-2697
ISSN	0022-149X
DOI	10.1017/S0022149X13000497
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Article ; Online: Stromal architecture directs early dissemination in pancreatic ductal adenocarcinoma

Arja Ray / Mackenzie K. Callaway / Nelson J. Rodríguez-Merced / Alexandra L. Crampton / Marjorie Carlson / Kenneth B. Emme / Ethan A. Ensminger / Alexander A. Kinne / Jonathan H. Schrope / Haley R. Rasmussen / Hong Jiang / David G. DeNardo / David K. Wood / Paolo P. Provenzano

JCI Insight, Vol 7, Iss

2022 Volume 3

Abstract	Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here, in both murine and human PDA, we demonstrate that extracellular matrix architecture regulates cell extrusion and subsequent invasion from intact ductal structures through tumor-associated collagen signatures (TACS). This results in early dissemination from histologically premalignant lesions and continual invasion from well-differentiated disease, and it suggests TACS as a biomarker to aid in the pathologic assessment of early disease. Furthermore, we show that pancreatitis results in invasion-conducive architectures, thus priming the stroma prior to malignant disease. Analysis in potentially novel microfluidic-derived microtissues and in vivo demonstrates decreased extrusion and invasion following focal adhesion kinase (FAK) inhibition, consistent with decreased metastasis. Thus, data suggest that targeting FAK or strategies to reengineer and normalize tumor microenvironments may have roles not only in very early disease, but also for limiting continued dissemination from unresectable disease. Likewise, it may be beneficial to employ stroma-targeting strategies to resolve precursor diseases such as pancreatitis in order to remove stromal architectures that increase risk for early dissemination.
Keywords	Cell biology ; Oncology ; Medicine ; R
Subject code	610
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	American Society for Clinical investigation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Mechanisms of osmolyte release.

Kinne, R K

Contributions to nephrology

1998 Volume 123, Page(s) 34–49

MeSH term(s)	Animals ; Betaine/metabolism ; Calcium Signaling ; Cell Membrane Permeability ; Cell Polarity ; Diabetes Mellitus/physiopathology ; Glycerylphosphorylcholine/metabolism ; Humans ; Hypotonic Solutions/pharmacology ; Inositol/metabolism ; Ion Channel Gating/drug effects ; Ion Channels/drug effects ; Ion Channels/physiology ; Ion Transport/drug effects ; Kidney Tubules, Collecting/cytology ; Kidney Tubules, Collecting/drug effects ; Kidney Tubules, Collecting/metabolism ; Loop of Henle/cytology ; Loop of Henle/drug effects ; Loop of Henle/metabolism ; Osmotic Pressure ; Rabbits ; Rats ; Sorbitol/metabolism ; Taurine/metabolism
Chemical Substances	Hypotonic Solutions ; Ion Channels ; Taurine (1EQV5MLY3D) ; Betaine (3SCV180C9W) ; Inositol (4L6452S749) ; Sorbitol (506T60A25R) ; Glycerylphosphorylcholine (60M22SGW66)
Language	English
Publishing date	1998-10-15
Publishing country	Switzerland
Document type	Journal Article ; Review
ISSN	0302-5144
ISSN	0302-5144
DOI	10.1159/000059927
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Article: Endothelial and epithelial cells: general principles of selective vectorial transport.

Kinne, R K

International journal of microcirculation, clinical and experimental

1997 Volume 17, Issue 5, Page(s) 223–230

Abstract: Endothelial and epithelial cells are both barriers and bridges between different compartments. This contribution discusses the general principles of paracellular, transcellular, and transmembrane transport with special emphasis on the relation between ... ...

Abstract	Endothelial and epithelial cells are both barriers and bridges between different compartments. This contribution discusses the general principles of paracellular, transcellular, and transmembrane transport with special emphasis on the relation between asymmetry and net movement of small solutes. Asymmetry of cell membrane transport properties is found in both epithelial and endothelial cell layers and provides the basis for transcellular transport. Furthermore, the asymmetry of membrane transporters such as the blood-brain barrier GLUT1 and the renal sodium-glutamate cotransporter is discussed with regard to their different properties at the extra- and intracellular face. These molecular asymmetries play an important role in the efficiency, direction, and regulation of transport processes across the plasma membranes in endothelial and epithelial cells.
MeSH term(s)	Amino Acid Transport System X-AG ; Animals ; Biological Transport ; Carrier Proteins/metabolism ; Endothelium/metabolism ; Epithelium/metabolism ; Glucose Transporter Type 1 ; Glutamate Plasma Membrane Transport Proteins ; Humans ; Kidney/metabolism ; Monosaccharide Transport Proteins/metabolism ; Symporters
Chemical Substances	Amino Acid Transport System X-AG ; Carrier Proteins ; Glucose Transporter Type 1 ; Glutamate Plasma Membrane Transport Proteins ; Monosaccharide Transport Proteins ; SLC2A1 protein, human ; Symporters
Language	English
Publishing date	1997-10
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	603169-9
ISSN	0167-6865
ISSN	0167-6865
DOI	10.1159/000179234
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Article ; Online: Functional role of the KCa3.1 potassium channel in synovial fibroblasts from rheumatoid arthritis patients.

Friebel, Kristin / Schönherr, Roland / Kinne, Raimund W / Kunisch, Elke

Journal of cellular physiology

2014 Volume 230, Issue 7, Page(s) 1677–1688

Abstract: Rheumatoid arthritis synovial fibroblasts (RA-SFs) show an aggressive phenotype and support joint inflammation and tissue destruction. New druggable targets in RA-SFs would therefore be of high therapeutic interest. The present study shows that the ... ...

Abstract	Rheumatoid arthritis synovial fibroblasts (RA-SFs) show an aggressive phenotype and support joint inflammation and tissue destruction. New druggable targets in RA-SFs would therefore be of high therapeutic interest. The present study shows that the intermediate-conductance, calcium-activated potassium channel KCa3.1 (KCNN4) is expressed at the mRNA and protein level in RA-SFs, is functionally active, and has a regulatory impact on cell proliferation and secretion of pro-inflammatory and pro-destructive mediators. Whole-cell patch-clamp recordings identified KCa3.1 as the dominant potassium channel in the physiologically relevant membrane voltage range below 0 mV. Stimulation with transforming growth factor β1 (TGF-β1) significantly increased transcription, translation, and channel function of KCa3.1. Inhibition of KCa3.1 by the selective, pore-blocking inhibitor TRAM-34, (and, in part, by siRNA) significantly reduced cell proliferation, as well as expression and secretion of pro-inflammatory factors (IL-6, IL-8, and MCP1) and the tissue-destructive protease MMP3. These effects were observed in non-stimulated and/or TGF-β1-stimulated RA-SFs. Since small molecule-based interference with KCa3.1 is principally well tolerated in clinical settings, further evaluation of channel blockers in models of rheumatoid arthritis may be a promising approach to identify new pharmacological targets and develop new therapeutic strategies for this debilitating disease.
MeSH term(s)	Arthritis, Rheumatoid/metabolism ; Arthritis, Rheumatoid/pathology ; Cytokines/genetics ; Cytokines/metabolism ; Fibroblasts/physiology ; Gene Expression Regulation/physiology ; Humans ; Intermediate-Conductance Calcium-Activated Potassium Channels/genetics ; Intermediate-Conductance Calcium-Activated Potassium Channels/metabolism ; Metalloproteases/genetics ; Metalloproteases/metabolism ; Pyrazoles/pharmacology ; RNA Interference ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering ; Reverse Transcriptase Polymerase Chain Reaction ; Synovial Membrane/chemistry ; Synovial Membrane/cytology
Chemical Substances	Cytokines ; Intermediate-Conductance Calcium-Activated Potassium Channels ; KCNN4 protein, human ; Pyrazoles ; RNA, Messenger ; RNA, Small Interfering ; TRAM 34 ; Metalloproteases (EC 3.4.-)
Language	English
Publishing date	2014-12-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.24924
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Article: Amino acid transporters.

Kinne, R K

Current opinion in nephrology and hypertension

1995 Volume 4, Issue 5, Page(s) 412–415

Abstract: This review focuses on two main aspects of amino acid transport. One aspect is the role of the basic amino acid transporter gene in causing cystinuria, its functional properties, and its potential transport functions. The other is the regulation of amino ...

Abstract	This review focuses on two main aspects of amino acid transport. One aspect is the role of the basic amino acid transporter gene in causing cystinuria, its functional properties, and its potential transport functions. The other is the regulation of amino acid transporters at the levels of information processing and cellular organization.
MeSH term(s)	Amino Acid Transport Systems ; Amino Acids/metabolism ; Animals ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Humans ; Ion Transport/physiology ; Kidney Tubules/metabolism
Chemical Substances	Amino Acid Transport Systems ; Amino Acids ; Carrier Proteins
Language	English
Publishing date	1995-09
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1151092-4
ISSN	1535-3842 ; 1062-4821 ; 1062-4813
ISSN (online)	1535-3842
ISSN	1062-4821 ; 1062-4813
DOI	10.1097/00041552-199509000-00007
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