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  1. Article: Metabolomic Approach to Study the 'Purple Queen' Pomegranate Cultivar Response to Alternative Culture Media and Phenological Stages.

    Martínez-Nicolás, Juan José / Hernández, Francisca / Núñez-Gómez, Dámaris / García-Sánchez, Francisco / Martínez-Font, Rafael / Legua, Pilar / Melgarejo, Pablo

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 2

    Abstract: The increasingly evident threat of depletion of world peat bogs is encouraging the search for and study of alternative agricultural substrates that can fully or partially replace peat, guaranteeing food supply (quality and quantity). On the other hand, ... ...

    Abstract The increasingly evident threat of depletion of world peat bogs is encouraging the search for and study of alternative agricultural substrates that can fully or partially replace peat, guaranteeing food supply (quality and quantity). On the other hand, the identification of the potential for the reuse of waste from relevant economic activities has increased in recent years, mainly motivated by the change to a sustainable circular economy, as is the case of port sediments. Taking into account that significant volumes of dredged port sediments are generated annually so that ports can maintain their economic activity, it is necessary to find objective, sustainable and safe reuse alternatives. In this sense, the objective of this study was to study the response of the "Purple Queen" pomegranate when grown with dredged port sediment. For this, the fruit production (kg), number of fruits (fruits tree-1), fruit weight (g), and seed yield (%) aiming to verify the correct tree development were evaluated. In addition, a 1H-NMR foliar metabolomic study for the three most relevant phenological phases was performed (flowering, fruit development, and post-harvest) to identify metabolic changes in trees. In total, 29 metabolites were identified; among them, 11 were amino acids, 6 organic acids, 5 sugars, and 7 secondary metabolites. The good agronomical development of the trees and fruits indicated the potential for using the dredged sediment as an agricultural substrate. On the other hand, the results revealed that the greatest variability in the metabolomic study occurred between the phenological phases and a lower variability is explained by the substrates used.
    Language English
    Publishing date 2023-01-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12020352
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Effects of marine sediment as agricultural substrate on soil microbial diversity: an amplicon sequencing study.

    Núñez-Gómez, Dámaris / Melgarejo, Pablo / Martínez-Nicolás, Juan José / Hernández, Francisca / Martínez-Font, Rafael / Lidón, Vicente / Legua, Pilar

    Environmental microbiome

    2023  Volume 18, Issue 1, Page(s) 69

    Abstract: Background: The soil microbiota has a direct impact on plant development and other metabolic systems, such as the degradation of organic matter and the availability of microelements and metabolites. In the context of agricultural soils, microbial ... ...

    Abstract Background: The soil microbiota has a direct impact on plant development and other metabolic systems, such as the degradation of organic matter and the availability of microelements and metabolites. In the context of agricultural soils, microbial activity is crucial for maintaining soil health and productivity. Thus, the present study aimed to identify, characterize, and quantify the microbial communities of four types of substrates with varying proportions of marine port sediment used for cultivating lemons. By investigating microbial diversity and relative abundance, the work aimed to highlight the importance of soil microbial communities in agriculture when alternative culture media was used.
    Results: The composition and structure of the sampled microbial communities were assessed through the amplification and sequencing of the V3-V4 variable regions of the 16 S rRNA gene The results revealed a diverse microbial community composition in all substrate samples, with a total of 41 phyla, 113 classes, 266 orders, 405 families, 715 genera, and 1513 species identified. Among these, Proteobacteria, Bacteroidota, Planctomycetota, Patescibacteria, Chloroflexi, Actinobacteriota, Acidobacteriota, Verrucomicrobiota, and Gemmatimonadota accounted for over 90% of the bacterial reads, indicating their dominance in the substrates.
    Conclusions: The impact of the substrate origin on the diversity and relative abundace of the microbiota was confirmed. The higher content of beneficial bacterial communities for plant development identified in peat could explain why is considered an ideal agricultural substrate. Development of "beneficial for plants" bacterial communities in alternative agricultural substrates, regardless of the edaphic characteristics, opens the possibility of studying the forced and specific inoculation of these culture media aiming to be agriculturally ideals.
    Language English
    Publishing date 2023-08-07
    Publishing country England
    Document type Journal Article
    ISSN 2524-6372
    ISSN (online) 2524-6372
    DOI 10.1186/s40793-023-00519-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Inhibition effect of polyurethane foam waste in dioxin formation.

    Moreno, Ana Isabel / Font, Rafael / Gomez-Rico, M Francisca

    Waste management (New York, N.Y.)

    2019  Volume 97, Page(s) 19–26

    Abstract: The present work has been focused on studying the polychlorinated polychlorodibenzo-p-dioxin/furan (PCDD/F) inhibition in the combustion process by prior addition of an inhibitor to the fuel. Three different experiments of combustion were carried out at ... ...

    Abstract The present work has been focused on studying the polychlorinated polychlorodibenzo-p-dioxin/furan (PCDD/F) inhibition in the combustion process by prior addition of an inhibitor to the fuel. Three different experiments of combustion were carried out at 850 °C in a laboratory-scale horizontal tubular quartz reactor, and several compounds were analyzed from the flue gas obtained, such as PCDD/Fs, dioxin-like polychlorinated biphenyls (dl-PCBs), NH
    MeSH term(s) Air Pollutants ; Benzofurans ; Dibenzofurans ; Dibenzofurans, Polychlorinated ; Dioxins ; Incineration ; Polychlorinated Biphenyls ; Polychlorinated Dibenzodioxins ; Polyurethanes
    Chemical Substances Air Pollutants ; Benzofurans ; Dibenzofurans ; Dibenzofurans, Polychlorinated ; Dioxins ; Polychlorinated Dibenzodioxins ; Polyurethanes ; polyurethane foam (9009-54-5) ; Polychlorinated Biphenyls (DFC2HB4I0K)
    Language English
    Publishing date 2019-07-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2001471-5
    ISSN 1879-2456 ; 0956-053X
    ISSN (online) 1879-2456
    ISSN 0956-053X
    DOI 10.1016/j.wasman.2019.07.034
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Life Cycle Assessment (LCA) of Substrate Mixes Containing Port Sediments for Sustainable 'Verna' Lemon Production.

    Hernández, Francisca / Martínez-Nicolás, Juan José / Melgarejo, Pablo / Núñez-Gómez, Dámaris / Lidón, Vicente / Martínez-Font, Rafael / Legua, Pilar

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 19

    Abstract: The increase in maritime trade and its global economic importance have forced port management actors to carry out the periodic dredging of their sediments to maintain an adequate depth for the passage of large ships to maintain their operation and ... ...

    Abstract The increase in maritime trade and its global economic importance have forced port management actors to carry out the periodic dredging of their sediments to maintain an adequate depth for the passage of large ships to maintain their operation and competitiveness. During the dredging process, large volumes of port sediment are generated. Dredged port sediment is currently considered a waste material and its disposal is regulated. Finding ways to safely reuse port sediments is necessary for sustainable development. In this study, a life cycle assessment (LCA) methodology was applied to identify the environmental impact of port sediments when used as a culture medium for lemon trees. A total of 90 lemon trees (
    Language English
    Publishing date 2022-10-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11193053
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Physico-Chemical Attributes of Lemon Fruits as Affected by Growing Substrate and Rootstock.

    Martínez-Nicolas, Juan José / Núñez-Gómez, Dámaris / Lidón, Vicente / Martínez-Font, Rafael / Melgarejo, Pablo / Hernández, Francisca / Legua, Pilar

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 16

    Abstract: Due to its high content of bioactive compounds, the lemon is considered one of the most relevant species around the world. Its great economic importance is motivated, in addition to its fresh consumption, by its applications in the medical, ... ...

    Abstract Due to its high content of bioactive compounds, the lemon is considered one of the most relevant species around the world. Its great economic importance is motivated, in addition to its fresh consumption, by its applications in the medical, pharmaceutical, and food industries, etc. However, the chemical and nutritional composition of lemon is not constant and can be influenced by external factors such as variety, weather conditions, crop management, etc. Determining the compositional variations of the fruit, essential to defining its potential use, was the main objective of this study. The physicochemical characteristics of the ‘Verna’ lemon were studied as a function of two controlled variables, the growing substrate and the rootstock. For this, 90 lemon trees were cultivated in three rootstocks and three different culture media. Lemon trees cultivated with 50% sediment/peat mix substrate presented a higher total production (590 lemons and 90.53 kg) while this production was 80% lower on trees cultivated with 75% marine sediment. Citrus macrophylla and Citrus aurantium/Citrus sinensis rootstocks showed a significantly higher production than the Citrus aurantium. All the fruits presented a predominantly yellow color appropriate for the market (0 < CI < +5). Nutritional and chemical parameters were consistent with data reported for the ‘Verna’ clones. All the obtained lemons were suitable for marketing and consumption both in fresh and processed forms. The results indicated the limited influence that the studied variables have on the quality parameters of lemon fruits, but they also could confirm the potential of marine sediment as a culture substrate.
    Language English
    Publishing date 2022-08-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11162487
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: In Vivo and In Vitro Assays Evaluating the Biological Activity of Taurine, Glucose and Energetic Beverages.

    Mateo-Fernández, Marcos / Valenzuela-Gómez, Fernando / Font, Rafael / Del Río-Celestino, Mercedes / Merinas-Amo, Tania / Alonso-Moraga, Ángeles

    Molecules (Basel, Switzerland)

    2021  Volume 26, Issue 8

    Abstract: Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be ... ...

    Abstract Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be evaluated in order to fully comprehend the potential impact of these products on the health due to the fact nutrition is closely related to science since the population consumes food to prevent certain diseases. Therefore, the aim of this study was to evaluate the biological effects of taurine, glucose, classic Red Bull
    MeSH term(s) Animals ; Artificially Sweetened Beverages/analysis ; Caffeine/analysis ; Carbonated Beverages/analysis ; Cell Survival/drug effects ; Comet Assay ; Cytotoxins/pharmacology ; DNA Fragmentation/drug effects ; DNA Methylation/drug effects ; Dietary Supplements/analysis ; Drosophila melanogaster/drug effects ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Energy Drinks/analysis ; Female ; Glucose/pharmacology ; HL-60 Cells ; Humans ; Longevity/drug effects ; Male ; Taurine/pharmacology
    Chemical Substances Cytotoxins ; Taurine (1EQV5MLY3D) ; Caffeine (3G6A5W338E) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2021-04-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26082198
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Metabolomic Approach to Study the ‘Purple Queen’ Pomegranate Cultivar Response to Alternative Culture Media and Phenological Stages

    Martínez-Nicolás, Juan José / Hernández, Francisca / Núñez-Gómez, Dámaris / García Sánchez, Francisco / Martínez-Font, Rafael / Legua, Pilar / Melgarejo, P.

    Foods. 2023 Jan. 11, v. 12, no. 2

    2023  

    Abstract: The increasingly evident threat of depletion of world peat bogs is encouraging the search for and study of alternative agricultural substrates that can fully or partially replace peat, guaranteeing food supply (quality and quantity). On the other hand, ... ...

    Abstract The increasingly evident threat of depletion of world peat bogs is encouraging the search for and study of alternative agricultural substrates that can fully or partially replace peat, guaranteeing food supply (quality and quantity). On the other hand, the identification of the potential for the reuse of waste from relevant economic activities has increased in recent years, mainly motivated by the change to a sustainable circular economy, as is the case of port sediments. Taking into account that significant volumes of dredged port sediments are generated annually so that ports can maintain their economic activity, it is necessary to find objective, sustainable and safe reuse alternatives. In this sense, the objective of this study was to study the response of the “Purple Queen” pomegranate when grown with dredged port sediment. For this, the fruit production (kg), number of fruits (fruits tree-1), fruit weight (g), and seed yield (%) aiming to verify the correct tree development were evaluated. In addition, a 1H-NMR foliar metabolomic study for the three most relevant phenological phases was performed (flowering, fruit development, and post-harvest) to identify metabolic changes in trees. In total, 29 metabolites were identified; among them, 11 were amino acids, 6 organic acids, 5 sugars, and 7 secondary metabolites. The good agronomical development of the trees and fruits indicated the potential for using the dredged sediment as an agricultural substrate. On the other hand, the results revealed that the greatest variability in the metabolomic study occurred between the phenological phases and a lower variability is explained by the substrates used.
    Keywords circular economy ; cultivars ; food availability ; fruit weight ; fruiting ; fruits ; metabolomics ; nuclear magnetic resonance spectroscopy ; peat ; phenology ; pomegranates ; secondary metabolites ; sediments ; seed yield ; trees ; wastes
    Language English
    Dates of publication 2023-0111
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12020352
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Lemon Peel and Juice: Metabolomic Differentiation

    Melgarejo, P. / Núñez-Gómez, Dámaris / Hernández, Francisca / Martínez-Font, Rafael / Lidón Noguera, Vicente / Martínez-Nicolás, Juan José / Legua, Pilar

    Horticulturae. 2023 Apr. 20, v. 9, no. 4

    2023  

    Abstract: Lemon is one of the most significant crops globally, with annual production exceeding 20.8 million tons in 2021. Spain leads the production in Europe with over 62% of lemon production (1.17 million tons in 2021). This study evaluated the real impact of ... ...

    Abstract Lemon is one of the most significant crops globally, with annual production exceeding 20.8 million tons in 2021. Spain leads the production in Europe with over 62% of lemon production (1.17 million tons in 2021). This study evaluated the real impact of cultivation conditions (rootstock and culture medium) on the compositional characteristics of ‘Verna’ lemons (peel and juice) using ¹H-MNR metabolomic identification techniques and multivariate analyses. Twenty metabolites were identified in both the peel and juice samples. Arginine, phenylalanine, ethanol, and trigonelline were absent in the peel samples but present in all the juice. On the other hand, the metabolites asparagine, glutamate, formate, and malate were present in the peel samples but absent in the juice. The analysis of the results indicates that the rootstock had a significant impact on the metabolites related to the energy metabolism of the plant, which directly affects the development of fruits and the influence of the culture conditions (rootstock and culture medium) on the plant’s adaptive response and modification of metabolic pathways.
    Keywords arginine ; asparagine ; culture media ; energy metabolism ; ethanol ; formates ; glutamic acid ; juices ; lemon peels ; lemons ; malates ; metabolites ; metabolomics ; phenylalanine ; rootstocks ; trigonelline ; Spain
    Language English
    Dates of publication 2023-0420
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2813983-5
    ISSN 2311-7524
    ISSN 2311-7524
    DOI 10.3390/horticulturae9040510
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Potential of dredged bioremediated marine sediment for strawberry cultivation.

    Martínez-Nicolás, Juan José / Legua, Pilar / Núñez-Gómez, Dámaris / Martínez-Font, Rafael / Hernández, Francisca / Giordani, Edgardo / Melgarejo, Pablo

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 19878

    Abstract: For the maintenance of the economic activity of the ports, it is necessary to dredge the marine sediments in order to guarantee their depth. These sediments, considered by European legislation as residues, present relevant limitations of use and generate ...

    Abstract For the maintenance of the economic activity of the ports, it is necessary to dredge the marine sediments in order to guarantee their depth. These sediments, considered by European legislation as residues, present relevant limitations of use and generate environmental and economic problems concerning their final disposal. In this context, the present work aims to identify the phytoremediated dredged sediments potential as an alternative to the traditional substrate (peat) in horticultural growing through two-years controlled strawberry cultivation. The growing media mixes used were: (1) 100% peat (Pt) as a control substrate; (2) 100% dredged remediated sediment (DRS); (3) 50% each (Pt-DRS). The dredged sediment, plant drainage and strawberry plant parts (leaves, stems, roots, and fruits) were analyzed to mineral elements, heavy metal contents, and pesticide residues (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and specific fumigants) during the experimental period. Only seven (Mn, Fe, Zn, Mo, Al, Mn and Ni) of the twenty-two metals and two (nitrates and fluorene) of the six hundred-thirteen pesticides analyzed were detected in the strawberry fruits. In all the cases, values detected were under the Spanish and European legal limit. The suitability of strawberry fruits for fresh and/or processed consumption with no risk was confirmed. Based on the results, can be affirmed that the dredged remediated sediment can be used as a culture substrate, alone or mixed with other substrates. Additional researches should be carried out to confirm the sediment characteristics and compare with other substrates to improve the physical and chemical properties.
    Language English
    Publishing date 2020-11-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-76714-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: In Vivo and In Vitro Assays Evaluating the Biological Activity of Taurine, Glucose and Energetic Beverages

    Marcos Mateo-Fernández / Fernando Valenzuela-Gómez / Rafael Font / Mercedes Del Río-Celestino / Tania Merinas-Amo / Ángeles Alonso-Moraga

    Molecules, Vol 26, Iss 2198, p

    2021  Volume 2198

    Abstract: Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be ... ...

    Abstract Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be evaluated in order to fully comprehend the potential impact of these products on the health due to the fact nutrition is closely related to science since the population consumes food to prevent certain diseases. Therefore, the aim of this study was to evaluate the biological effects of taurine, glucose, classic Red Bull ® and sugar-free Red Bull ® in order to check the food safety and the nutraceutical potential of these compounds, characterising different endpoints: (i) Toxicology, antitoxicology, genotoxicology and life expectancy assays were performed in the Drosophila melanogaster model organism; (ii) The in vitro chemopreventive activity of testing compounds was determined by assessing their cytotoxicity, the proapoptotic DNA-damage capability to induce internucleosomal fragmentation, the strand breaks activity and the modulator role on the methylation status of genomic repetitive sequences of HL-60 promyelocytic cells. Whereas none tested compounds showed toxic or genotoxic effect, all tested compounds exerted antitoxic and antigenotoxic activity in Drosophila . Glucose, classic Red Bull ® and sugar-free Red Bull ® were cytotoxic in HL-60 cell line. Classic Red Bull ® induced DNA internucleosomal fragmentation although none of them exhibited DNA damage on human leukaemia cells. In conclusion, the tested compounds are safe on Drosophila melanogaster and classic Red Bull ® could overall possess nutraceutical potential in the in vivo and in vitro model used in this study. Besides, taurine could holistically be one of the bioactive compounds responsible for the biological activity of classic Red Bull ® .
    Keywords classic Red Bull ® ; taurine ; glucose ; Drosophila melanogaster ; HL-60 cell line ; Organic chemistry ; QD241-441
    Subject code 333
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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