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Article ; Online: Cellular and molecular insights into incomplete immune recovery in HIV/AIDS patients.

Yan, Liting / Xu, Kaiju / Xiao, Qing / Tuo, Lin / Luo, Tingting / Wang, Shuqiang / Yang, Renguo / Zhang, Fujie / Yang, Xingxiang

Frontiers in immunology

2023 Volume 14, Page(s) 1152951

Abstract: Highly active antiretroviral therapy (ART) can effectively inhibit virus replication and restore immune function in most people living with human immunodeficiency virus (HIV). However, an important proportion of patients fail to achieve a satisfactory ... ...

Abstract	Highly active antiretroviral therapy (ART) can effectively inhibit virus replication and restore immune function in most people living with human immunodeficiency virus (HIV). However, an important proportion of patients fail to achieve a satisfactory increase in CD4
MeSH term(s)	Humans ; HIV ; HIV Infections ; CD4 Lymphocyte Count ; Antiretroviral Therapy, Highly Active/adverse effects ; CD4-Positive T-Lymphocytes
Language	English
Publishing date	2023-05-02
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1152951
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Article ; Online: Effect of probiotic intervention on intestinal flora and immune status in patients with secondary infections to HBV-related acute-on-chronic liver failure

YANG Renguo / XU Kaiju / ZHOU Qiaoling / YANG Xingxiang

Di-san junyi daxue xuebao, Vol 41, Iss 8, Pp 794-

2019 Volume 798

Abstract: Objective To assess the effect of probiotic intervention on intestinal flora and immune status in patients with secondary infections to HBV-related acute-on-chronic liver failure (HBV-ACLF). Methods A total of 101 patients with HBV-ACLF treated in our ... ...

Abstract	Objective To assess the effect of probiotic intervention on intestinal flora and immune status in patients with secondary infections to HBV-related acute-on-chronic liver failure (HBV-ACLF). Methods A total of 101 patients with HBV-ACLF treated in our hospital between January, 2017 and June, 2018 were enrolled in this study, including 56 without secondary infections (HBV-ACLF group) and 45 with secondary infections to HBV-ACLF (secondary infection group), with 67 healthy participants as controls. All the patients with HBV-ACLF were treated with bifidobacterium quadruple live bacteria tablets in addition to the routine treatments for 1 month. From all the participants, fecal specimens were collected before and after the treatment to analyze the changes in Lactobacillus, Bifidobacterium, Escherichia coli, Enterococcus, yeast, Staphylococcus, Bacteroides and Streptococcus mutans; peripheral blood samples were also obtained for testing the serum levels of immunoglobulin A (IgA), IgG and IgM using ELISA; the changes in T lymphocyte subsets CD3+, CD4+ and CD8+ were detected using immunofluorescence flow cytometry, and the levels of C-reactive protein (CRP), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) and interleukin-8(IL-8) were determined using ELISA. Results Compared with the control group, the patients with HBV-ACLF in both groups before the treatment showed significantly reduced abundance of intestinal Lactobacilli and Bifidobacteria and increased abundance of Escherichia coli, Enterococcus, yeast, Staphylococcus, Bacteroides, and Streptococcus pneumoniae (P < 0.05), with significantly lowered levels of IgA, IgG, IgM, NK, CD4+ and CD4+/CD8+ (P < 0.05) and elevated levels of CRP, TNF-α, IL-6 and IL-8 (P < 0.05); These changes were even more obvious in patients with secondary infections than in those without infections (P < 0.05). After the treatment, the intestinal Lactobacilli, Bifidobacteria and blood levels of IgA, IgG, IgM, NK, CD4+ and CD4+/CD8+ increased significantly (P < 0.05), and ...
Keywords	hbv-related acute-on-chronic liver failure ; probiotics ; infection ; intestinal flora ; immune status ; Medicine (General) ; R5-920
Subject code	610
Language	Chinese
Publishing date	2019-04-01T00:00:00Z
Publisher	Editorial Office of Journal of Third Military Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Osthole improves collagen-induced arthritis in a rat model through inhibiting inflammation and cellular stress.

Xu, Renguo / Liu, Zhen / Hou, Jiande / Huang, Tao / Yang, Ming

Cellular & molecular biology letters

2018 Volume 23, Page(s) 19

Abstract: Background: Osthole is a natural product that has multiple bioactive functions and has been reported to exert potent immunosuppressive effects. However, the therapeutic effect of osthole on arthritis has not been explored. In the present study, a ... ...

Abstract	Background: Osthole is a natural product that has multiple bioactive functions and has been reported to exert potent immunosuppressive effects. However, the therapeutic effect of osthole on arthritis has not been explored. In the present study, a collagen-induced arthritis rat model, IL-1β-stimulated SW982 cells, and RA-like fibroblast-like synoviocytes (FLS) were employed to investigate the effect and possible mechanism of osthole on arthritis in vivo and in vitro. Results: 20 and 40 mg/kg osthole significantly alleviated collagen-induced arthritic symptoms based on histopathology and clinical arthritis scores, and improved erosion using HE staining. 20 and 40 mg/kg osthole decreased the level of IL-1β, TNF-α and IL-6 in rats and ameliorated oxidative stress in serum evaluated using ELISA kits. In addition, treatment with 50 and 100 μM osthole for 48 h inhibited 10 ng/ml IL-1β-stimulated proliferation and migration of SW982, and significantly inhibited the expression of matrix metalloproteinases, such as MMP-1, MMP-3 and MMP-13, as detected by western blot. 50 and 100 μM osthole also blocked the generation of IL-6 and TNF-α in IL-1β-stimulated SW982 cells. The NF-κB and MAPK pathways were also inhibited by osthole in IL-1β-treated SW982 cells. Conclusion: These results collectively demonstrated that osthole improves collagen-induced arthritis in a rat model and IL-1β-treated SW982 cells through inhibiting inflammation and cellular stress in vivo and in vitro, and osthole might be a promising therapeutic agent for RA.
MeSH term(s)	Animals ; Anti-Inflammatory Agents, Non-Steroidal/administration & dosage ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Arthritis, Experimental/chemically induced ; Arthritis, Experimental/drug therapy ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Coumarins/administration & dosage ; Coumarins/pharmacology ; Coumarins/therapeutic use ; Hindlimb/pathology ; Humans ; Inflammation/chemically induced ; Inflammation/drug therapy ; Interleukin-1beta/blood ; Interleukin-6/blood ; Male ; Oxidative Stress/drug effects ; Rats ; Rats, Sprague-Dawley ; Synoviocytes/drug effects ; Tumor Necrosis Factor-alpha/blood
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Coumarins ; Interleukin-1beta ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; osthol (XH1TI1759C)
Language	English
Publishing date	2018-05-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2108724-6
ISSN	1689-1392 ; 1689-1392
ISSN (online)	1689-1392
ISSN	1689-1392
DOI	10.1186/s11658-018-0086-0
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Article ; Online: Antimicrobial Bioresorbable Mg-Zn-Ca Alloy for Bone Repair in a Comparison Study with Mg-Zn-Sr Alloy and Pure Mg.

Zhang, Chaoxing / Lin, Jiajia / Nguyen, Nhu-Y Thi / Guo, Yuxing / Xu, Changlu / Seo, Catherine / Villafana, Edgar / Jimenez, Hector / Chai, Yang / Guan, Renguo / Liu, Huinan

ACS biomaterials science & engineering

2019 Volume 6, Issue 1, Page(s) 517–538

Abstract: Magnesium-zinc-calcium (Mg-Zn-Ca) alloys have attracted increasing attention for biomedical implant applications, especially for bone repair, because of their biocompatibility, biodegradability, and similar mechanical properties to human bone. The ... ...

Abstract	Magnesium-zinc-calcium (Mg-Zn-Ca) alloys have attracted increasing attention for biomedical implant applications, especially for bone repair, because of their biocompatibility, biodegradability, and similar mechanical properties to human bone. The objectives of this study were to characterize Mg-2 wt % Zn-0.5 wt % Ca (named ZC21) alloy pins microstructurally and mechanically, and determine their degradation and interactions with host cells and pathogenic bacteria in vitro and in vivo in comparison with the previously studied Mg-4 wt % Zn-1 wt % strontium (named ZSr41) alloy and Mg control. Specifically, the in vitro degradation and cytocompatibility of ZC21 pins with bone marrow derived mesenchymal stem cells (BMSCs) were investigated using both direct culture and direct exposure culture methods. The adhesion density of BMSCs on ZC21 pins (i.e., direct contact) was significantly higher than on pure Mg pins in both in vitro culture methods; the cell adhesion density around ZC21 pins (i.e., indirect contact) was similar to the cell-only positive control in both in vitro culture methods. Interestingly, ZC21 showed a higher daily degradation rate, crack width and crack area ratio in the direct exposure culture than in the direct culture, suggesting different culture methods did affect its in vitro degradation behaviors. When cultured with Gram-positive bacteria methicillin-resistant
MeSH term(s)	Absorbable Implants ; Alloys ; Anti-Bacterial Agents ; Methicillin-Resistant Staphylococcus aureus ; Zinc/pharmacology
Chemical Substances	Alloys ; Anti-Bacterial Agents ; Mg-Zn-Sr alloy ; Zinc (J41CSQ7QDS)
Language	English
Publishing date	2019-12-31
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.9b00903
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Article ; Online: Shikonin protects chondrocytes from interleukin-1beta-induced apoptosis by regulating PI3K/Akt signaling pathway.

Wang, Leisheng / Gai, Pengzhou / Xu, Renguo / Zheng, Yanpin / Lv, Shiqiao / Li, Yu / Liu, Shaoxian

International journal of clinical and experimental pathology

2015 Volume 8, Issue 1, Page(s) 298–308

Abstract: Chondrocyte apoptosis is mostly responsible for the development and progression of osteoarthritis. IL-1β is generally served as an agent that induces chondrocyte apoptosis. Shikonin exerts its anti-inflammatory effect on cartilage protection in vivo. We ... ...

Abstract	Chondrocyte apoptosis is mostly responsible for the development and progression of osteoarthritis. IL-1β is generally served as an agent that induces chondrocyte apoptosis. Shikonin exerts its anti-inflammatory effect on cartilage protection in vivo. We aimed to explore the protective effect of shikonin on interleukin-1beta (IL-1β)-induced chondrocyte apoptosis and the potential molecular mechanisms. Chondrocytes were isolated from the joints of newborn Sprague-Dawley rats. The MTT assay and LDH cell death assay were used to determine the cell viability and chondrocyte apoptosis was detected by Annexin-V/PI staining and nucleosomal degradation. The contents of phosphorylated-PI3K (p-PI3k), phosphorylated-Akt (p-Akt), Bcl-2, Bax, and cytochrome c were detected by Western blotting. A quantitative colorimetric assay was used to detect the caspase-3 activity. Our results showed that pretreatment with shikonin (4 μM) inhibited cytotoxicity and apoptosis induced by IL-1β (10 ng/ml) in chondrocytes. Shikonin pretreatment also decreased the activity of IL-1β that decreased Bcl-2 expression and levels of p-PI3K and p-Akt, and increased Bax expression, cytochrome c release, and caspase-3 activation. It also reversed the activity of IL-1β that promoted the synthesis of matrix metalloproteinase-13 and inhibited the expression of tissue inhibitor of metalloproteinase-1 expression, with the net effect of suppressing extracellular matrix degradation. These data suggested that shikonin may protect chondrocytes from apoptosis induced by IL-1β through the PI3K/Akt signaling pathway, by deactivating caspase-3.
MeSH term(s)	Animals ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Apoptosis/drug effects ; Blotting, Western ; Cell Survival/drug effects ; Cells, Cultured ; Chondrocytes/drug effects ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Male ; Naphthoquinones/pharmacology ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/drug effects
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Naphthoquinones ; shikonin (3IK6592UBW) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2015-01-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2418306-4
ISSN	1936-2625 ; 1936-2625
ISSN (online)	1936-2625
ISSN	1936-2625
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Article: A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.

Song, Dandan / Qu, Xiaofeng / Liu, Yushen / Li, Li / Yin, Dehui / Li, Juan / Xu, Kun / Xie, Renguo / Zhai, Yue / Zhang, Huiwen / Bao, Hao / Zhao, Chao / Wang, Juan / Song, Xiuling / Song, Wenzhi

Nanoscale research letters

2017 Volume 12, Issue 1, Page(s) 179

Abstract: Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection ... ...

Abstract	Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 10
Language	English
Publishing date	2017-03-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	2253244-4
ISSN	1556-276X ; 1931-7573
ISSN (online)	1556-276X
ISSN	1931-7573
DOI	10.1186/s11671-017-1941-z
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Article: Fibroscan improves the diagnosis sensitivity of liver fibrosis in patients with chronic hepatitis B.

Huang, Rengang / Jiang, Nan / Yang, Renguo / Geng, Xiaoxia / Lin, Jianmei / Xu, Gang / Liu, Dandan / Chen, Jidog / Zhou, Guo / Wang, Shuqiang / Luo, Tingting / Wu, Jiazhen / Liu, Xiaoshu / Xu, Kaiju / Yang, Xingxiang

Experimental and therapeutic medicine

2016 Volume 11, Issue 5, Page(s) 1673–1677

Abstract: The aim of the present study was to investigate the diagnostic accuracy of Fibroscan for liver fibrosis in patients with chronic hepatitis B (CHB) with alanine aminotransferase (ALT) levels <2 times the upper normal limit. A total of 263 consecutive ... ...

Abstract	The aim of the present study was to investigate the diagnostic accuracy of Fibroscan for liver fibrosis in patients with chronic hepatitis B (CHB) with alanine aminotransferase (ALT) levels <2 times the upper normal limit. A total of 263 consecutive patients with CHB and ALT levels <2 times the upper normal limit were enrolled in the present study. Liver biopsies and liver stiffness measurements (LSM) were conducted. Receiver operating characteristic (ROC) analysis was used to determine the predictive ability of LSM for the development of liver fibrosis in patients with stage S1, S2 and S3 liver fibrosis. Bivariate Spearman rank correlation analysis was performed in order to determine the association between liver stiffness value, which was measured by Fibroscan, and liver fibrosis stage, which was measured by liver biopsy. The liver stiffness value was found to be positively correlated with the liver fibrosis stage (r=0.522, P<0.001) and necroinflammatory activity (r=0.461, P<0.001), which was measured by liver biopsy. The optimal cut-off value in the patients with stage S1, S2 and S3 liver fibrosis was 5.5, 8.0 and 10.95 kPa, respectively. The area under the ROC curve for the prediction of the development of liver fibrosis in these patients was 0.696, 0.911 and 0.923, respectively. The threshold of the optimal cut-off value exhibited a high sensitivity and specificity. The results of the present study suggested that Fibroscan may improve the sensitivity of the diagnosis of liver fibrosis in patients with CHB and ALT levels <2 times the upper normal limit, and that this sensitivity may increase with the progression of liver fibrosis.
Language	English
Publishing date	2016-03-09
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2683844-8
ISSN	1792-1015 ; 1792-0981
ISSN (online)	1792-1015
ISSN	1792-0981
DOI	10.3892/etm.2016.3135
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Article: Calculation theory and experiment verification of sedimentation potential of the complex particle system

Fang, Ying-Guang / Liu, Hao / Guo, Ling-Feng / Li, Xiao-Long / Wang, Pei-Xu / Gu, Ren-guo

Colloids and surfaces. 2022 Sept. 20, v. 649

2022

Abstract: In this paper, the equations for calculating the sedimentation potential of complex particle systems composed of solid-liquid mixtures are established, and the multifactorial coupling effects are also taken into consideration, especially different types ... ...

Abstract	In this paper, the equations for calculating the sedimentation potential of complex particle systems composed of solid-liquid mixtures are established, and the multifactorial coupling effects are also taken into consideration, especially different types and shapes of particles in the system, suspended particle concentration, ion concentration, solid-fluid interaction, as well as the coupling effect of the electric field induced by the sedimentation current, etc. In this context, the theoretical calculation results are proved to be consistent with the test results. The equations established in the paper are of substantial value in exploring the principles of particle behavior control in the fields of the chemical industry, metallurgy, pharmacy, air pollution, water environment treatment, etc. Moreover, this paper further discovers the sedimentation potential characteristics of the "time-switch" function of the uniform particle system, and based on this finding, the logical calculation unit is constructed, which can be expected to have a wide range of potential applications in the fields of hydrology, atmosphere, water quality, and other intelligent monitoring.
Keywords	air pollution ; chemical industry ; electric field ; hydrology ; metallurgy ; water quality
Language	English
Dates of publication	2022-0920
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500517-3
ISSN	0927-7757
ISSN	0927-7757
DOI	10.1016/j.colsurfa.2022.129447
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Article: [Kinematics of the triangular fibrocartilage complex during forearm rotation in vivo].

Xu, Jing / Tang, Jin-bo / Jia, Zhong-zheng / Xie, Ren-guo

Zhonghua wai ke za zhi [Chinese journal of surgery

2009 Volume 47, Issue 21, Page(s) 1647–1650

Abstract: Objective: To investigate three-dimensional kinematics of the superficial and deep portion of triangular fibrocartilage complex (TFCC) in different parts of the forearm rotation.: Methods: Six wrists of 6 volunteers were used to obtain CT scans at ... ...

Abstract	Objective: To investigate three-dimensional kinematics of the superficial and deep portion of triangular fibrocartilage complex (TFCC) in different parts of the forearm rotation. Methods: Six wrists of 6 volunteers were used to obtain CT scans at different positions of the wrist. The wrists were scanned from 90 degrees of pronation to 90 degrees of supination at an interval of 30 degrees. The 3-dimensional radius and ulna were reconstructed with customized software and changes in length of the superficial and deep portion of TFCC during forearm rotation. Results: In forearm pronation, the superficial dorsal portion and the deep palmar portion of the TFCC were tight. While the superficial palmar portion and the deep dorsal potion of the TFCC were lax. In supination, the changes in length of all these fibers were reverse. Conclusions: In forearm rotation one portion fibers of dorsal TFCC and one portion fibers of palmar TFCC are tight, and this mechanism controls stability during DRUJ rotation.
MeSH term(s)	Adult ; Biomechanical Phenomena ; Female ; Forearm/physiology ; Humans ; Imaging, Three-Dimensional ; Male ; Pronation ; Radiography ; Radius/diagnostic imaging ; Supination ; Triangular Fibrocartilage/diagnostic imaging ; Ulna/diagnostic imaging ; Wrist Joint/diagnostic imaging ; Young Adult
Language	Chinese
Publishing date	2009-11-01
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604573-x
ISSN	0529-5815
ISSN	0529-5815
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Zs.B 2469: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 218: Show issues

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Article: Acid-mediated [3+3] cycloaddition of α-EWG-α-formyl ketene-S,S-acetals and α-carbamoyl ketene-S,S-acetals: a new approach to 2-pyridone derivatives

Han, Xiao-Dan / Guo-Liang Xu / Hui-Bin Wang / Jian-Ping Fu / Ju-Wu Hu / Ren-Guo Zhu / Wei Xiong / Xiong-Hui Li / Zhao-Yang Deng

Tetrahedron letters. 2015 Nov. 18, v. 56, no. 46

2015

Abstract: A series of 2-pyridone derivatives have been synthesized via a formal [3+3] annulation strategy starting from readily available α-EWG-α-formyl ketene-S,S-acetal 1 and α-carbamoyl ketene-S,S-acetals 2 in the presence of CH3COOH at 80°C in excellent yields ...

Abstract	A series of 2-pyridone derivatives have been synthesized via a formal [3+3] annulation strategy starting from readily available α-EWG-α-formyl ketene-S,S-acetal 1 and α-carbamoyl ketene-S,S-acetals 2 in the presence of CH3COOH at 80°C in excellent yields (91–98%). A mechanism involving sequential Baylis–Hillman reaction, intramolecular cycloaddition, Michael addition, and alkylthiol elimination processes for this novel reaction is described in the Letter. In addition, substituted 1-aryl pyridine-2(1H)-ones 4 have also been prepared in high yields via a Cu(OAc)2-mediated three-component reaction of 1, 2, and aryl boronic acid.
Keywords	acetic acid ; chemical structure ; cycloaddition reactions
Language	English
Dates of publication	2015-1118
Size	p. 6444-6447.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	204287-3
ISSN	1873-3581 ; 0040-4039
ISSN (online)	1873-3581
ISSN	0040-4039
DOI	10.1016/j.tetlet.2015.09.152
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