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  1. Article ; Online: Correlation between negative life events and suicide attempts among Yi adolescents with HIV/AIDS in Liangshan Prefecture.

    Song, Xiaozhen / Zou, Shoukang / Li, Yangling / Chen, Liyu / Feng, Ping / Xu, Peiwei / Zhang, Hang / Deng, Fang / Xu, Hanmei / Tao, Yuanmei / Jin, Meijiang / Ran, Maojia / Zhang, Hong / Ma, Fanghua / Wang, Ying / Yin, Li

    BMC public health

    2023  Volume 23, Issue 1, Page(s) 1600

    Abstract: ... exercise, drug therapy and suicide attempts.: Methods: A total of 180 Yi adolescents aged 11-19 years ... that the incidence rate of suicide attempts among Yi adolescents with HIV/AIDS in Liangshan Prefecture was 13.9%. Negative ... of suicide attempts among Yi adolescents with HIV/AIDS in Liangshan Prefecture is high and should be considered ...

    Abstract Objective: To investigate the incidence of suicide attempts among adolescents with HIV/AIDS in Liangshan Prefecture, Sichuan Province, as well as the correlation between negative life events, sleep, exercise, drug therapy and suicide attempts.
    Methods: A total of 180 Yi adolescents aged 11-19 years with HIV/AIDS in a county of Liangshan Prefecture, Sichuan Province, China, were investigated by census. The main outcome indicators included the incidence of suicide attempts and whether negative life events, sleep, exercise, drug therapy and other factors were related to suicide attempts.
    Results: We found that the incidence rate of suicide attempts among Yi adolescents with HIV/AIDS in Liangshan Prefecture was 13.9%. Negative life events were a risk factor for suicide attempts (OR = 1.047, p < 0.001, 95% CI 1.027-1.067). In the factors of negative life events, adaptation was a risk factor for suicide attempts (OR = 1.203, p = 0.026, 95% CI 1.022-1.416), and academic pressure showed a tendency to be a risk factor for suicide attempts (OR = 1.149, p = 0.077, 95% CI 0.985-1.339). However, the punishment factor, interpersonal stress factor and loss factor had no significant correlation with suicide attempts. There was no significant correlation between sleep, exercise, drug therapy and suicide attempts.
    Conclusion: The proportion of suicide attempts among Yi adolescents with HIV/AIDS in Liangshan Prefecture is high and should be considered. Negative life events are independent risk factors for suicide attempts, and it is necessary to strengthen the screening and early intervention for suicide attempts in HIV/AIDS adolescents with definite negative life events.
    MeSH term(s) Humans ; Adolescent ; Suicide, Attempted ; Acclimatization ; Acquired Immunodeficiency Syndrome ; Censuses ; China/epidemiology
    Language English
    Publishing date 2023-08-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041338-5
    ISSN 1471-2458 ; 1471-2458
    ISSN (online) 1471-2458
    ISSN 1471-2458
    DOI 10.1186/s12889-023-16377-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A screening strategy for bioactive components of Bu-Zhong-Yi-Qi-Tang regulating spleen-qi deficiency based on "endobiotics-targets-xenobiotics" association network.

    Hu, Liufang / Chen, Jiali / Duan, Huifang / Zou, Zhenyu / Qiu, Yuan / Du, Jing / Chen, Jiaxu / Yao, Xinsheng / Kiyohara, Hiroaki / Nagai, Takayuki / Yao, Zhihong

    Journal of ethnopharmacology

    2023  Volume 314, Page(s) 116605

    Abstract: Ethnopharmacological relevance: Bu-Zhong-Yi-Qi-Tang is a famous traditional Chinese medicine ... Yi-Qi-Tang.: Materials and methods: The effects of Bu-Zhong-Yi-Qi-Tang were evaluated ... of Bu-Zhong-Yi-Qi-Tang in the bio-samples were characterized using ultra-high-performance ...

    Abstract Ethnopharmacological relevance: Bu-Zhong-Yi-Qi-Tang is a famous traditional Chinese medicine formula that has been prevalent in China for over 700 years to treat spleen-qi deficiency related diseases, such as gastrointestinal and respiratory disorders. However, the bioactive components responsible for regulating spleen-qi deficiency remain unclear and have puzzled many researchers.
    Aim of the study: The current study focuses on efficacy evaluation of regulating spleen-qi deficiency and screening the bioactive components of Bu-Zhong-Yi-Qi-Tang.
    Materials and methods: The effects of Bu-Zhong-Yi-Qi-Tang were evaluated through blood routine examination, immune organ index, and biochemical analysis. Metabolomics was utilized to analyze the potential endogenous biomarkers (endobiotics) in the plasma, and the prototypes (xenobiotics) of Bu-Zhong-Yi-Qi-Tang in the bio-samples were characterized using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Then, these endobiotics were used as "bait" to predict targets based on network pharmacology and to screen potential bioactive components from the absorbed prototypes in the plasma by constructing an "endobiotics-targets-xenobiotics" association network. Further, the anti-inflammatory activities of representative compounds (calycosin and nobiletin) were validated through poly(I:C)-induced pulmonary inflammation mice model.
    Results: Bu-Zhong-Yi-Qi-Tang exhibited immunomodulatory and anti-inflammatory activities in spleen-qi deficiency rat, as supported by the observation of increased levels of D-xylose and gastrin in serum, an increase in the thymus index and number of lymphocytes in blood, as well as a reduction in the level of IL-6 in bronchoalveolar lavage fluid. Furthermore, plasma metabolomic analysis revealed a total of 36 Bu-Zhong-Yi-Qi-Tang related endobiotics, which were mainly enriched in primary bile acids biosynthesis, the metabolism of linoleic acid, and the metabolism of phenylalanine pathways. Meanwhile, 95 xenobiotics were characterized in plasma, urine, small intestinal contents, and tissues of spleen-qi deficiency rat after Bu-Zhong-Yi-Qi-Tang treatment. Using an integrated association network, six potential bioactive components of Bu-Zhong-Yi-Qi-Tang were screened. Among them, calycosin was found to significantly reduce the levels of IL-6 and TNF-α in the bronchoalveolar lavage fluid, increase the number of lymphocytes, while nobiletin dramatically decreased the levels of CXCL10, TNF-α, GM-CSF, and IL-6.
    Conclusion: Our study proposed an available strategy for screening bioactive components of BYZQT regulating spleen-qi deficiency based on "endobiotics-targets-xenobiotics" association network.
    MeSH term(s) Mice ; Rats ; Animals ; Spleen ; Tumor Necrosis Factor-alpha/pharmacology ; Interleukin-6 ; Xenobiotics/pharmacology ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Drugs, Chinese Herbal/chemistry ; Anti-Inflammatory Agents/pharmacology
    Chemical Substances bu-zhong-yi-qi-tang ; Tumor Necrosis Factor-alpha ; Interleukin-6 ; Xenobiotics ; Drugs, Chinese Herbal ; Anti-Inflammatory Agents
    Language English
    Publishing date 2023-05-11
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2023.116605
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genetic polymorphism investigation of the Chinese Yi minority using PowerPlex® Y23 STR amplification system.

    He, GuangLin / Chen, PengYu / Zou, Xing / Chen, Xu / Song, Feng / Yan, Jing / Hou, YiPing

    International journal of legal medicine

    2017  Volume 131, Issue 3, Page(s) 663–666

    Abstract: ... healthy male individuals from the Yi minority population residing in the Liangshan Yi Autonomous ... Prefecture, Sichuan, China. A total of 179 alleles and 297 haplotypes were discovered in the Yi group ... between the Yi minority group and 10 Asian meta-populations comprising 58 individual populations were ...

    Abstract Twenty-three Y-STR loci (DYS576, DYS389I, DYS389 II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS393, DYS458 DYS456, DYS643, Y-GATA-H4, and DYS385a/b) included in the next-generation PowerPlex® Y23 System were first investigated in 311 unrelated, healthy male individuals from the Yi minority population residing in the Liangshan Yi Autonomous Prefecture, Sichuan, China. A total of 179 alleles and 297 haplotypes were discovered in the Yi group. In total, 285 haplotypes among them were unique, and the remaining 12 haplotypes were observed in two or three individuals. Haplotype discrimination capacity and haplotype diversity were 0.9550 and 0.9989, respectively. Genetic diversity ranged from 0.4550 (DYS437) to 0.9556 (DYS385a/b). Population comparisons between the Yi minority group and 10 Asian meta-populations comprising 58 individual populations were performed. Both multidimensional scaling plots and phylogenetic analyses demonstrated that the genetic structure of the Chinese Yi ethnicity was extremely different compared to Taiwan indigenous inhabitants among 10 Asian meta-populations. Additionally, the genetic structure resemblance of the Yi group was obtained from a geographically close population (Xuanwei Han) or similar language family groups (Thai populations). Besides, our study has demonstrated that the PowerPlex® Y23 System has high polymorphism in a Chinese Yi ethnic population and high discriminatory power for forensic purposes. Population data of the 23 Y-STR obtained from a Yi ethnic population has enriched the Chinese ethnic genetic information.
    MeSH term(s) China ; Chromosomes, Human, Y ; Ethnic Groups/genetics ; Genetics, Population ; Haplotypes ; Humans ; Male ; Microsatellite Repeats ; Multiplex Polymerase Chain Reaction/instrumentation ; Polymorphism, Genetic
    Language English
    Publishing date 2017-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-017-1537-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Allele frequencies of 15 autosomal STRs in Chinese Nakhi and Yi populations.

    He, Guanglin / Su, Yongdong / Zou, Xing / Wang, Mengge / Liu, Jing / Wang, Shouyu / Hou, Yiping / Wang, Zheng

    International journal of legal medicine

    2018  Volume 133, Issue 1, Page(s) 105–108

    Abstract: ... Both Nakhi and Yi had the genetically close relationships with Yunnan Bai and distinct relationships ...

    Abstract Genetic characterization of ethnically and geographically diverse populations via short tandem repeats (STRs) is relevant to various fundamental and applied areas of forensic genetics, population studies, and even molecular anthropology. In the present study, genetic polymorphisms of 15 autosomal STR loci were firstly obtained from 918 individuals (495 Nakhis and 423 Yis) residing in the foothills of the Himalayas. The cumulative powers of discrimination and probabilities of exclusion in the two studied ethnic groups were both larger than 0.999999999999999982 and 0.9999961, respectively. Genetic similarities and differences among 61 populations were subsequently investigated by pairwise Cavalli-Sforza genetic distance, multidimensional scaling plots, principal component analysis, and phylogenetic relationship reconstruction. Both Nakhi and Yi had the genetically close relationships with Yunnan Bai and distinct relationships with Xinjiang Turkic-speaking populations (Uyghur and Kazakh) and Vietnamese.
    MeSH term(s) Asian Continental Ancestry Group/genetics ; China ; DNA Fingerprinting ; Gene Frequency ; Genetics, Population ; Humans ; Microsatellite Repeats ; Polymorphism, Genetic
    Language English
    Publishing date 2018-09-14
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-018-1931-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Jian-Pi-Yi-Shen Formula Ameliorates Oxidative Stress, Inflammation, and Apoptosis by Activating the Nrf2 Signaling in 5/6 Nephrectomized Rats.

    Zhou, Fanyuan / Zou, Xiaohu / Zhang, Jing / Wang, Ziwei / Yang, Yajun / Wang, Dongtao

    Frontiers in pharmacology

    2021  Volume 12, Page(s) 630210

    Abstract: ... mortality. Jian-Pi-Yi-Shen (JPYS) formula is a representative traditional Chinese medicine formula ...

    Abstract Chronic kidney disease (CKD) is an increasing global public health problem, with high morbidity and mortality. Jian-Pi-Yi-Shen (JPYS) formula is a representative traditional Chinese medicine formula in the treatment of CKD, which is widely used in clinical practice in China. However, the underlying mechanism has not been well elucidated. In the present study, we measured the markers of apoptosis, inflammation, oxidative stress, and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling to investigate the effects of JPYS formula on renal function and fibrosis and its molecular mechanism in an established animal model of 5/6 nephrectomized (5/6Nx) rats. The results demonstrated that the JPYS formula exerted a significant preventive effect on renal dysfunction and fibrosis, based on analysis of correlative parameters such as urinary protein, SCr, BUN, glomerular sclerosis index, and tubulointerstitial fibrosis score and renal histopathology and ultrastructural pathology of CKD rats. JPYS formula also induced downregulation of gene expression associated with fibrosis, such as TGF-β and type I, III, and IV collagen. Moreover, the JPYS formula showed a significant protective effect in suppressing cell apoptosis according to the results of apoptotic indexes, including increased gene expression of Bcl-2, decreased gene expression of Bax, caspase 3, caspase 9, and the number of TUNEL-positive cells. JPYS formula also ameliorated the activation of the NF-κB-mediated inflammatory pathway, as manifested by the downregulation of gene expression of TNF-α, IL-1β, IκBα, NF-κB p65, MCP-1, CXCL1, COX-2, and iNOS in the kidney. Our evidence also suggested that the JPYS formula ameliorates oxidative stress by promoting antioxidant function according to antioxidant index indicators as an indicator of GSH, SOD, CAT, and GPx and abating excessive accumulation of the reactive oxygen species biomarkers, including ROS, TBARS, 8-oxo-dG, and MDA. The data also suggested that the JPYS formula reversed the downregulation of HO-1 and Nrf2 level and upregulation of Keap1 expression. Together, our data highlighted that the JPYS formula relieved renal oxidative injury mediated by activation of Nrf2 signaling by inhibiting inflammation and apoptosis in CKD rats.
    Language English
    Publishing date 2021-03-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2021.630210
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Identification and Quantification of Bu Shen Yi Sui Capsule by UPLC-LTQ-Orbitrap-MSn and UPLC-QTOF-MS/MS.

    Liu, Haolong / Zhao, Yiyi / Zheng, Qi / Yang, Tao / Zhao, Hui / Zou, Haiyan / Fan, Yongping / Guo, Hongzhu / Wang, Lei

    Journal of chromatographic science

    2021  Volume 60, Issue 5, Page(s) 450–457

    Abstract: ... Yi Sui capsule (BSYSC), which is used in treating multiple sclerosis as a kind of TCMs. Under ...

    Abstract Traditional Chinese medicines (TCMs) have been considered as important alternative therapeutics because of their significant medicinal benefits in specific diseases. Chinese herb formula is characterized by a vast molecule that differs in routine medicines. Due to TCMs chemical complexity, proper quality control has been a great challenge. Choosing the appropriate method to identify and qualify these compounds is an important work to ensure its safety, efficacy and quality control. Thus, this study aimed at providing novel information on high-resolution LTQ-Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap-MSn) based identification of Bu Shen Yi Sui capsule (BSYSC), which is used in treating multiple sclerosis as a kind of TCMs. Under the proposed chromatographic conditions, 80 chemical components classified as anthraquinone, phenolic acid and phenylethanoid glycosides were separated and identified from BSYSC. Coupled with the high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method, eight of them were regarded as marker compounds for the quantitative evaluation of BSYSC. The identification and quantification with precision of UPLC-LTQ-Orbitrap-MSn and UPLC-QTOF-MS/MS could facilitate essential data for further pharmacokinetic studies of BSYSC.
    MeSH term(s) Busulfan ; Chromatography, High Pressure Liquid/methods ; Drugs, Chinese Herbal ; Quality Control ; Tandem Mass Spectrometry
    Chemical Substances Drugs, Chinese Herbal ; Busulfan (G1LN9045DK)
    Language English
    Publishing date 2021-07-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80141-0
    ISSN 1945-239X ; 0021-9665
    ISSN (online) 1945-239X
    ISSN 0021-9665
    DOI 10.1093/chromsci/bmab091
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Identification of the Active Constituents and Significant Pathways of Shen-qi-Yi-zhu Decoction on Antigastric Cancer: A Network Pharmacology Research and Experimental Validation.

    Zeng, Shuhong / Yu, Zhibao / Xu, Xintian / Liu, Yuanjie / Li, Jiepin / Zhao, Danya / Song, Changjuan / Lu, Haixia / Zhao, Yudong / Lu, Weimin / Zou, Xi

    Evidence-based complementary and alternative medicine : eCAM

    2021  Volume 2021, Page(s) 6642171

    Abstract: Shen-qi-Yi-zhu decoction (SQYZD) is an empirical prescription with antigastric cancer (GC) property ...

    Abstract Shen-qi-Yi-zhu decoction (SQYZD) is an empirical prescription with antigastric cancer (GC) property created by Xu Jing-fan, a National Chinese Medical Master. However, its underlying mechanisms are still unclear. Based on network pharmacology and experimental verification, this study puts forward a systematic method to clarify the anti-GC mechanism of SQYZD. The active ingredients of SQYZD and their potential targets were acquired from the TCMSP database. The target genes related to GC gathered from GeneCards, DisGeNET, OMIM, TTD, and DrugBank databases were imported to establish protein-protein interaction (PPI) networks in GeneMANIA. Cytoscape was used to establish the drug-ingredients-targets-disease network. The hub target genes collected from the SQYZD and GC were parsed via GO and KEGG analysis. Our findings from network pharmacology were successfully validated using an
    Language English
    Publishing date 2021-11-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2021/6642171
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Genetic diversity and phylogenetic structure of four Tibeto-Burman-speaking populations in Tibetan-Yi corridor revealed by insertion/deletion polymorphisms.

    Zou, Xing / He, Guanglin / Wang, Mengge / Huo, Liwen / Chen, Xu / Liu, Jing / Wang, Shouyu / Ye, Ziwei / Wang, Fei / Wang, Zheng / Hou, Yiping

    publication RETRACTED

    Molecular genetics & genomic medicine

    2020  Volume 8, Issue 4, Page(s) e1140

    Abstract: ... population genetics and forensic application.: Methods: Thirty InDels in 530 individuals residing in the Tibetan-Yi ... powers of exclusion were 0.9807 (Dujiangyan Tibetan), 0.9880 (Muli Tibetan), 0.9852 (Xichang Yi) and 0 ... 999999999942 (Muli Tibetan), 0.999999999982 (Xichang Yi) and 0.999999999962 (Yanyuan Mosuo), respectively ...

    Abstract Background: Insertion/deletion polymorphisms (InDels), combined with all the desirable features of both short tandem repeat and single nucleotide polymorphism, have been used in archaeological and anthropological research, population genetics and forensic application.
    Methods: Thirty InDels in 530 individuals residing in the Tibetan-Yi corridor (142 Dujiangyan Tibetans, 164 Muli Tibetans, 187 Xichang Yis, and 37 Yanyuan Mosuos) were genotyped using the Investigator DIPplex. Forensic parameters and allele frequency spectrum were calculated. Genetic relationships between the investigated populations and worldwide and nationwide populations were assessed based on both the allele frequency distribution and genotype data.
    Results: The combined powers of exclusion were 0.9807 (Dujiangyan Tibetan), 0.9880 (Muli Tibetan), 0.9852 (Xichang Yi) and 0.9892 (Yanyuan Mosuo). The combined powers of discrimination were 0.999999999983 (Dujiangyan Tibetan), 0.999999999942 (Muli Tibetan), 0.999999999982 (Xichang Yi) and 0.999999999962 (Yanyuan Mosuo), respectively. The comprehensive population comparisons among worldwide and nationwide populations uniformly illustrated that the investigated populations have a genetically closer relationship with Tibeto-Burman-speaking populations and geographically adjacent populations.
    Conclusion: These 30 loci can be regarded as an efficient genetic tool in forensic individual identification and as a supplementary tool in paternity testing in Dujiangyan Tibetan, Muli Tibetan, Xichang Yi, and Yanyuan Mosuo. The genetic proximity between the four populations in the Tibetan-Yi corridor and other populations is strongly correlated with the linguistic origin and geographical distance.
    MeSH term(s) China ; Ethnicity/genetics ; Forensic Genetics/methods ; Humans ; INDEL Mutation ; Phylogeny ; Polymorphism, Genetic ; Population/genetics
    Language English
    Publishing date 2020-02-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication
    ZDB-ID 2734884-2
    ISSN 2324-9269 ; 2324-9269
    ISSN (online) 2324-9269
    ISSN 2324-9269
    DOI 10.1002/mgg3.1140
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Identification of the Active Constituents and Significant Pathways of Shen-qi-Yi-zhu Decoction on Antigastric Cancer

    Shuhong Zeng / Zhibao Yu / Xintian Xu / Yuanjie Liu / Jiepin Li / Danya Zhao / Changjuan Song / Haixia Lu / Yudong Zhao / Weimin Lu / Xi Zou

    Evidence-Based Complementary and Alternative Medicine, Vol

    A Network Pharmacology Research and Experimental Validation

    2021  Volume 2021

    Abstract: Shen-qi-Yi-zhu decoction (SQYZD) is an empirical prescription with antigastric cancer (GC) property ...

    Abstract Shen-qi-Yi-zhu decoction (SQYZD) is an empirical prescription with antigastric cancer (GC) property created by Xu Jing-fan, a National Chinese Medical Master. However, its underlying mechanisms are still unclear. Based on network pharmacology and experimental verification, this study puts forward a systematic method to clarify the anti-GC mechanism of SQYZD. The active ingredients of SQYZD and their potential targets were acquired from the TCMSP database. The target genes related to GC gathered from GeneCards, DisGeNET, OMIM, TTD, and DrugBank databases were imported to establish protein-protein interaction (PPI) networks in GeneMANIA. Cytoscape was used to establish the drug-ingredients-targets-disease network. The hub target genes collected from the SQYZD and GC were parsed via GO and KEGG analysis. Our findings from network pharmacology were successfully validated using an in vitro HGC27 cell model experiment. In a word, this study proves that the combination of network pharmacology and in vitro experiments is effective in clarifying the potential molecular mechanism of traditional Chinese medicine (TCM).
    Keywords Other systems of medicine ; RZ201-999
    Subject code 570
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Yi Qi Jie Du Formula and Salinomycin Combination Treatment Mediates Nasopharyngeal Carcinoma Stem Cell Proliferation, Migration and Apoptosis via CD44/Ras Signaling Pathway

    He Lan / Zhou Fang-Liang / Zou Pan / Wang Xian-Wen / Jiang Yi-Lan / He Ying-Chun / Liao Duan-Fang / Cao De-Liang

    Digital Chinese Medicine, Vol 3, Iss 4, Pp 297-

    2020  Volume 308

    Abstract: Objective: To assess the effects of Yi Qi Jie Du Formula (YQJDF) combined with salinomycin (SAL ...

    Abstract Objective: To assess the effects of Yi Qi Jie Du Formula (YQJDF) combined with salinomycin (SAL) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying molecular mechanisms. Methods: Cell counting methods, the CCK-8 assay, transwell migration assay and JC-1 staining, were used to observe the effects of the combination on the proliferation, migration and apoptosis of NPC stem cells, respectively. Western blot was used to detect the levels of protein in NPC-SCs. Results: YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPC-SC apoptosis. Mechanistically, YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins, including cleaved Caspase-3, cleaved Caspase-7 and cleaved Caspase-9. Moreover, YQJDF combined with SAL synergistically decreased the levels of CD44, p-c-Src, Ras, p-PKCδ, p-MEK, p-c-Raf, p-ERK1/2 and p-AKT proteins. Conclusions: The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis, which may be closely related to the downregulation of the CD44/Ras signaling pathway.
    Keywords Yi Qi Jie Du Formula (YQJDF) ; Salinomycin ; Nasopharyngeal carcinoma stem cells (NPC-SCs) ; Proliferation ; Apoptosis ; CD44/Ras signaling pathway ; Medicine ; R ; Other systems of medicine ; RZ201-999
    Subject code 610
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher KeAi Communications Co., Ltd.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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