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  1. Article ; Online: Rates and types of injuries during the three consecutive years 2016 to 2018 of the Vätternrundan-One of the world's largest and longest bicycle races.

    Sillén, Karin / Wallenius, Ville

    Traffic injury prevention

    2019  Volume 20, Issue 7, Page(s) 749–752

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Adult ; Bicycling/injuries ; Female ; Humans ; Male ; Medical Records ; Retrospective Studies ; Sweden/epidemiology ; Wounds and Injuries/epidemiology ; Wounds and Injuries/therapy
    Language English
    Publishing date 2019-08-05
    Publishing country England
    Document type Journal Article ; Observational Study
    ZDB-ID 2089818-6
    ISSN 1538-957X ; 1538-9588
    ISSN (online) 1538-957X
    ISSN 1538-9588
    DOI 10.1080/15389588.2019.1639681
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: βHB inhibits glucose-induced GLP-1 secretion in GLUTag and human jejunal enteroids.

    Elebring, Erik / Casselbrant, Anna / Persson, Sara M T / Fändriks, Lars / Wallenius, Ville

    Journal of molecular endocrinology

    2023  Volume 70, Issue 4

    Abstract: Ingestion of nutrients stimulates incretin secretion from enteroendocrine cells (EECs) of the epithelial layer of the gut. Glucagon-like peptide-1 (GLP-1) is one of these incretins that stimulate postprandial insulin release and signal satiety to the ... ...

    Abstract Ingestion of nutrients stimulates incretin secretion from enteroendocrine cells (EECs) of the epithelial layer of the gut. Glucagon-like peptide-1 (GLP-1) is one of these incretins that stimulate postprandial insulin release and signal satiety to the brain. Understanding the regulation of incretin secretion might open up new therapeutic options for obesity and type-2 diabetes mellitus. To investigate the inhibitory effect of the ketone body β-hydroxybutyrate (βHB) on glucose-induced GLP-1 secretion from EECs, in vitro cultures of murine GLUTag cells and differentiated human jejunal enteroid monolayers were stimulated with glucose to induce GLP-1 secretion. The effect of βHB on GLP-1 secretion was studied using ELISA and ECLIA methods. GLUTag cells stimulated with glucose and βHB were analysed using global proteomics focusing on cellular signalling pathways and the results were verified by Western blot. Results demonstrated βHB had a significant inhibitory effect on glucose-induced GLP-1 secretion at a dose of 100 mM in GLUTag cells. In differentiated human jejunal enteroid monolayers, glucose-induced secretion of GLP-1 was inhibited at a much lower dose of 10 mM βHB. The addition of βHB to GLUTag cells resulted in decreased phosphorylation of kinase AKT and transcription factor STAT3 and also influenced the expressions of signalling molecule IRS-2, kinase DGKε and receptor FFAR3. In conclusion, βHB displays an inhibitory effect on glucose-induced GLP-1 secretion in vitro in GLUTag cells and in differentiated human jejunal enteroid monolayers. This effect may be mediated through multiple downstream mediators of G-protein coupled receptor activation, such as PI3K signalling.
    MeSH term(s) Humans ; Mice ; Animals ; Glucagon-Like Peptide 1/pharmacology ; Glucagon-Like Peptide 1/metabolism ; Incretins/metabolism ; Glucose/pharmacology ; Glucose/metabolism ; 3-Hydroxybutyric Acid ; Receptors, G-Protein-Coupled/metabolism
    Chemical Substances Glucagon-Like Peptide 1 (89750-14-1) ; Incretins ; Glucose (IY9XDZ35W2) ; 3-Hydroxybutyric Acid (TZP1275679) ; Receptors, G-Protein-Coupled
    Language English
    Publishing date 2023-04-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 645012-x
    ISSN 1479-6813 ; 0952-5041
    ISSN (online) 1479-6813
    ISSN 0952-5041
    DOI 10.1530/JME-22-0115
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  3. Article: The value of "diaphragmatic relaxing incision" for the durability of the crural repair in patients with paraesophageal hernia: a double blind randomized clinical trial.

    Tsoposidis, A / Thorell, A / Axelsson, H / Reuterwall Hansson, M / Lundell, L / Wallenius, V / Kostic, S / Håkanson, B

    Frontiers in surgery

    2023  Volume 10, Page(s) 1265370

    Abstract: Background: Surgical repair of paraesophageal hernias (PEHs) is burdened with high recurrence rates, and hitherto various techniques explored to enforce the traditional crural repair have not been successful. The hiatal reconstruction in PEH is exposed ... ...

    Abstract Background: Surgical repair of paraesophageal hernias (PEHs) is burdened with high recurrence rates, and hitherto various techniques explored to enforce the traditional crural repair have not been successful. The hiatal reconstruction in PEH is exposed to significant tension, which may be minimized by adding a diaphragmatic relaxing incision to enhance the durability of the crural repair.
    Patients and methods: All individuals undergoing elective laparoscopic repair of a large PEH, irrespective of age, were considered eligible. PEHs were classified into types II-IV. The preoperative work-up program included multidetector computed tomography and symptom assessment questionnaires, which will be repeated during the postoperative follow-up. Patients were randomly divided into a control group with crural repair alone and an intervention group with the addition of a left-sided diaphragmatic relaxing incision at the edge of the upper pole of the spleen. The diaphragmatic defect was then covered by a synthetic mesh.
    Results: The primary endpoint of this trial was the rate of anatomical PEH recurrence at 1 year. Secondary endpoints included symptomatic gastroesophageal reflux disease, dysphagia, odynophagia, gas bloat, regurgitation, chest pain, abdominal pain, nausea, vomiting, postprandial pain, cardiovascular and pulmonary symptoms, and patient satisfaction in the immediate postoperative course (3 months) and at 1 year. Postoperative complications, morbidity, and disease burden were recorded for each patient. This was a double-blind study, meaning that the operation report was filed in a locked archive to keep the patient, staff, and clinical assessors blinded to the study group allocation. Blinding must not be broken during the follow-up unless required by any emergencies in the clinical management of the patient. Likewise, the patients must not be informed about the details of the operation.
    Trial registration: ClinicalTrials.gov, identification number NCT04179578.
    Language English
    Publishing date 2023-11-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2773823-1
    ISSN 2296-875X
    ISSN 2296-875X
    DOI 10.3389/fsurg.2023.1265370
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  4. Article: Glycocholic acid and butyrate synergistically increase vitamin D-induced calcium uptake in Caco-2 intestinal epithelial cell monolayers.

    Casselbrant, Anna / Fändriks, Lars / Wallenius, Ville

    Bone reports

    2020  Volume 13, Page(s) 100294

    Abstract: Background: Roux-en-Y gastric bypass (RYGB) substantially decreases intestinal calcium absorption and may eventually lead to bone resorption. This is likely a consequence of bile diversion from the alimentary limb, as the presence of bile seems ... ...

    Abstract Background: Roux-en-Y gastric bypass (RYGB) substantially decreases intestinal calcium absorption and may eventually lead to bone resorption. This is likely a consequence of bile diversion from the alimentary limb, as the presence of bile seems necessary for vitamin D-mediated calcium uptake. We recently suggested that the mediating mechanism may be a down-regulation of the vitamin D co-activator heat-shock protein (Hsp)90β. Recent evidence suggests that vitamin D may have effects on both active and passive calcium absorption.
    Aim: To identify mechanisms in vitro that may be responsible for the decreased calcium absorption after RYGB. We hypothesized that bile, alone or in concert with nutritional compounds, could be of importance.
    Material & methods: Caco-2 cells were grown confluent on semi-permeable membranes in a double-chamber setup to mimic small intestinal mucosa. The effect of bile acids chenodeoxycholic, lithocholic, glycocholic and taurocholic acid, with and without the addition of the fatty-acid butyrate, were tested for their effects on Hsp90β expression and active and passive calcium-flux monitored using radioactive
    Results: We initially found that whole human bile, but only together with the fatty acid butyrate, potently induced Hsp90β expression. In line with this, a single bile acid, e.g. glycocholic acid (GCA), in combination with butyrate, increased Hsp90β expression (40 ± 13% vs. GCA, butyrate or vehicle alone; p < 0,001; n = 14-25). Further, this combination together with vitamin D increased the passive gradient-driven flux of calcium, compared to stimulation with vitamin D alone or in combination with either GCA or butyrate (880 ± 217% vs. vitamin D and GCA or butyrate, or vitamin D only; p = 0,01-0.006; n = 5-11). Surprisingly, this combination had no effect on active calcium transport in the absence of calcium gradient.
    Conclusion: The combination of GCA and butyrate increased gradient-driven calcium uptake up to 9-fold in Caco-2 intestinal epithelial cells, but had no effect on active calcium absorption. This effect was mediated via the vitamin D receptor co-activator Hsp90β.
    Language English
    Publishing date 2020-07-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2821774-3
    ISSN 2352-1872
    ISSN 2352-1872
    DOI 10.1016/j.bonr.2020.100294
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  5. Article ; Online: Dags för kritisk bedömning av nya minimalinvasiva antirefluxtekniker.

    Håkanson, Bengt / Tsoposidis, Alexandros / Wallenius, Ville / Lundell, Lars / Kostic, Srdjan / Axelsson, Hans / Thorell, Anders

    Lakartidningen

    2022  Volume 119

    Abstract: GERD is the most prevalent gastrointestinal disorder in the Western world and the extent of anatomic alterations underlying the mechanisms of GERD can be viewed upon as a spectrum from a single anatomic alteration (e.g.  incompetent lower esophageal ... ...

    Title translation Novel endoscopic antireflux procedures - time for critical appraisal.
    Abstract GERD is the most prevalent gastrointestinal disorder in the Western world and the extent of anatomic alterations underlying the mechanisms of GERD can be viewed upon as a spectrum from a single anatomic alteration (e.g.  incompetent lower esophageal sphincter) to multiple anatomic alterations, such as diaphragmatic hiatal hernia. The degree of anatomic aberrations also seem to correlate with the complications of GERD. Since GERD is a heterogenous disease, it can be argued that its treatment should be individualized. The medical and surgical therapies have been the mainstay of long-term treatment of GERD, but during recent decades several Food and Drug Administration (FDA)-approved devices have become available for endoscopic treatment of GERD, thus potentially filling the alleged therapeutic gap between medication and surgery. Endoscopic treatment options are now considered appropriate treatment in particular in patients early in the GERD spectrum. However, serious methodological concerns can be raised regarding the scientific documentation behind all of these devices, despite the fact that they are vigorously marketed. This article outlines the basic principles and guidelines for the current and future documentations of such devices, which might be helpful for the clinician in selecting the most accurate long-term therapeutic alternative for patients with chronic GERD.
    MeSH term(s) Endoscopy/methods ; Esophageal Sphincter, Lower/surgery ; Gastroesophageal Reflux/drug therapy ; Gastroesophageal Reflux/surgery ; Hernia, Hiatal/surgery ; Humans
    Language Swedish
    Publishing date 2022-08-22
    Publishing country Sweden
    Document type Journal Article
    ZDB-ID 391010-6
    ISSN 1652-7518 ; 0023-7205
    ISSN (online) 1652-7518
    ISSN 0023-7205
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  6. Article ; Online: Effects of medical and surgical treatment on vitamin D levels in obesity.

    Mejaddam, Ala / Höskuldsdóttir, Gudrún / Lenér, Frida / Wallenius, Ville / Trimpou, Penelope / Fändriks, Lars / Mossberg, Karin / Eliasson, Björn / Landin-Wilhelmsen, Kerstin

    PloS one

    2023  Volume 18, Issue 12, Page(s) e0292780

    Abstract: Introduction: Persons living with obesity treated with bariatric surgery are at a high risk of developing nutritional deficiencies. The primary aim of this observational cohort study was to compare vitamin D levels in patients two years after bariatric ... ...

    Abstract Introduction: Persons living with obesity treated with bariatric surgery are at a high risk of developing nutritional deficiencies. The primary aim of this observational cohort study was to compare vitamin D levels in patients two years after bariatric surgery (Roux-en-Y gastric bypass/RYGB and sleeve gastrectomy/SG) with a very low-energy diet (VLED). The same subjects were also compared with a population sample from the same region at baseline. The primary hypothesis was that surgery, especially RYGB, would lead to an increased prevalence of vitamin D deficiency compared to subjects treated with VLED. 971 individuals eligible for surgical, RYGB (n = 388), SG (n = 201), and medical treatment (n = 382), in routine care, were included consecutively between 2015 and 2017. A random population sample from the WHO-MONICA project was used as a reference, (n = 414). S-calcium, S-25(OH)D (vitamin D), and S-PTH (parathyroid hormone) were measured in all persons with obesity at baseline and two years after treatment (n = 713). Self-reported use of vitamin D and calcium supplementation was registered.
    Results: Vitamin D deficiency (S-25(OH)D <25mmol/l) was found in 5.2% of the persons with obesity at baseline versus 1.7% of the general population (SMD>0.1). S-25(OH)D increased for all treatment groups but was higher in RYGB and SG (SMD>0.1, standardized mean difference). Thirteen subjects (1.8%) had vitamin D deficiency after obesity treatment.
    Conclusion: Surgical intervention for obesity followed by vitamin D supplementation was not associated with a higher risk for vitamin D deficiency, irrespective of surgery type, compared to individuals on medical treatment. However, persons living with obesity seeking weight loss treatment are more likely to have deficient vitamin D levels compared to the general population.
    MeSH term(s) Humans ; Vitamin D ; Obesity, Morbid/surgery ; Calcium ; Obesity/surgery ; Vitamins/therapeutic use ; Gastric Bypass ; Vitamin D Deficiency/drug therapy ; Vitamin D Deficiency/epidemiology ; Gastrectomy ; Retrospective Studies
    Chemical Substances Vitamin D (1406-16-2) ; Calcium (SY7Q814VUP) ; Vitamins
    Language English
    Publishing date 2023-12-22
    Publishing country United States
    Document type Observational Study ; Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0292780
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  7. Article ; Online: Anaemia in patients with self-reported use of iron supplements in the BAriatric surgery SUbstitution and nutrition study: A prospective cohort study.

    Lenér, Frida / Höskuldsdóttir, Gudrún / Landin-Wilhelmsen, Kerstin / Björkelund, Cecilia / Eliasson, Björn / Fändriks, Lars / Wallenius, Ville / Engström, My / Mossberg, Karin

    Nutrition, metabolism, and cardiovascular diseases : NMCD

    2023  Volume 33, Issue 5, Page(s) 998–1006

    Abstract: Background and aims: After bariatric surgery, micronutrient deficiencies may lead to anaemia. To prevent post-operative deficiencies, patients are recommended lifelong micronutrient supplementation. Studies investigating the effectiveness of ... ...

    Abstract Background and aims: After bariatric surgery, micronutrient deficiencies may lead to anaemia. To prevent post-operative deficiencies, patients are recommended lifelong micronutrient supplementation. Studies investigating the effectiveness of supplementation to prevent anaemia after bariatric surgery are scarce. This study aimed to investigate the relationship between nutritional deficiencies and anaemia in patients who report use of supplementation two years after bariatric surgery versus patients who do not.
    Methods and results: Obese (BMI≥35 kg/m
    Conclusion: The results from this study indicate that iron deficiency or anaemia may not be prevented by substitutional treatment per current guidelines after bariatric surgery and highlights there is reason to ensure adequate preoperative micronutrient levels.
    Trial registration: March 03, 2015; NCT03152617.
    MeSH term(s) Male ; Female ; Humans ; Iron/adverse effects ; Obesity, Morbid/diagnosis ; Obesity, Morbid/surgery ; Prospective Studies ; Self Report ; Bariatric Surgery/adverse effects ; Gastric Bypass/adverse effects ; Malnutrition ; Anemia/diagnosis ; Anemia/epidemiology ; Anemia/prevention & control ; Dietary Supplements/adverse effects ; Hemoglobins ; Gastrectomy/adverse effects ; Gastrectomy/methods ; Micronutrients
    Chemical Substances Iron (E1UOL152H7) ; Hemoglobins ; Micronutrients
    Language English
    Publishing date 2023-02-16
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1067704-5
    ISSN 1590-3729 ; 0939-4753
    ISSN (online) 1590-3729
    ISSN 0939-4753
    DOI 10.1016/j.numecd.2023.02.008
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  8. Article: Simultaneous Measurement of Tricarboxylic Acid Cycle Intermediates in Different Biological Matrices Using Liquid Chromatography-Tandem Mass Spectrometry; Quantitation and Comparison of TCA Cycle Intermediates in Human Serum, Plasma, Kasumi-1 Cell and Murine Liver Tissue.

    Rathod, Ramji / Gajera, Bharat / Nazir, Kenneth / Wallenius, Janne / Velagapudi, Vidya

    Metabolites

    2020  Volume 10, Issue 3

    Abstract: The tricarboxylic acid (TCA) cycle is a central part of carbon and energy metabolism, also connecting to glycolysis, amino acid, and lipid metabolism. The quantitation of the TCA cycle intermediate within one method is lucrative due to the interest in ... ...

    Abstract The tricarboxylic acid (TCA) cycle is a central part of carbon and energy metabolism, also connecting to glycolysis, amino acid, and lipid metabolism. The quantitation of the TCA cycle intermediate within one method is lucrative due to the interest in central carbon metabolism profiling in cells and tissues. In addition, TCA cycle intermediates in serum have been discovered to correspond as biomarkers to various underlying pathological conditions. In this work, an Liquid Chromatography-Mass Spectrometry/Mass Spectrometry-based quantification method is developed and validated, which takes advantage of fast, specific, sensitive, and cost-efficient precipitation extraction. Chromatographic separation is achieved while using Atlantis dC18 2.1 mm × 100 mm, particle size 3-μm of Waters column with a gradient elution mobile phase while using formic acid in water (0.1%
    Language English
    Publishing date 2020-03-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662251-8
    ISSN 2218-1989
    ISSN 2218-1989
    DOI 10.3390/metabo10030103
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  9. Article ; Online: Stor överförskrivning och ökat bruk av protonpumpshämmare.

    Agreus, Lars / Borgquist, Lars / Tsoposidis, Alexandros / Wallenius, Ville / Kostic, Srdjan / Lundell, Lars / Lindberg, Greger

    Lakartidningen

    2021  Volume 118

    Abstract: PPIs (Proton-pump inhibitors) offers the best treatment for acid related diseases. The predominant indications for PPI prescription are: GERD eradication of H. pylori-infection in combination with antibiotics H. pylori-negative peptic ulcer  healing of ... ...

    Title translation Significant over- and misuse of PPIs.
    Abstract PPIs (Proton-pump inhibitors) offers the best treatment for acid related diseases. The predominant indications for PPI prescription are: GERD eradication of H. pylori-infection in combination with antibiotics H. pylori-negative peptic ulcer  healing of and prophylaxis against NSAID/COXIB--induced gastroduodenal lesions  acid hypersecretory states such as Zollinger-Ellisons syndrome. The market for PPIs continues to expand in most countries. A significant over- and misuse of PPIs prevails in hospital care as well as in general practice. The predominant reasons for and mechanisms behind the over- and misuse of PPIs are well recognised. The most important consequences of this overprescription of PPIs are increasing medical costs and risk for long-term adverse side effects. Continued education and dedicated information are key factors to guide physicians, medical personnel and patients to adopt to generally accepted principles for and balanced use of PPIs.
    MeSH term(s) Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Helicobacter Infections/drug therapy ; Humans ; Peptic Ulcer/chemically induced ; Peptic Ulcer/prevention & control ; Proton Pump Inhibitors/adverse effects
    Chemical Substances Anti-Inflammatory Agents, Non-Steroidal ; Proton Pump Inhibitors
    Language Swedish
    Publishing date 2021-07-01
    Publishing country Sweden
    Document type Journal Article
    ZDB-ID 391010-6
    ISSN 1652-7518 ; 0023-7205
    ISSN (online) 1652-7518
    ISSN 0023-7205
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  10. Article ; Online: Morphological Adaptation in the Jejunal Mucosa after Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers: Data from a Randomized Crossover Study.

    Casselbrant, Anna / Wallenius, Ville / Elebring, Erik / Marschall, Hanns-Ulrich / Johansson, Bengt R / Helander, Herbert F / Fändriks, Lars

    Nutrients

    2022  Volume 14, Issue 19

    Abstract: Background and aims: The conditions for jejunal glucose absorption in healthy subjects have not been thoroughly studied. In this study we investigated differences in : Methods: A single-centre, randomized, unblinded crossover study in 15 healthy ... ...

    Abstract Background and aims: The conditions for jejunal glucose absorption in healthy subjects have not been thoroughly studied. In this study we investigated differences in
    Methods: A single-centre, randomized, unblinded crossover study in 15 healthy volunteers ingesting strictly controlled equicaloric diets (either HCD or HFD), with 60% energy from the respective source. An enteroscopy was carried out after 2 weeks of each diet and jejunal mucosal biopsies were acquired. Conventional histology, immunofluorescent staining, transmission electron microscopy and confocal microscopy were used.
    Results: The villi did not demonstrate any change in the epithelial enlargement factor. Despite an increased mitosis, there were no changes in apoptotic indices. However, the ultrastructural analysis demonstrated a significant increase in the enlargement factor at the bases of the villi. The mitochondria demonstrated increased amounts of cristae after the HFD. The confocal microscopy revealed increased HMGCS2 per mitochondrial marker at the top of the villi after the HFD compared to the HCD.
    Conclusion: There is a morphometric adaption in the jejunal mucosa following the 2-week diets, not only on a histological level, but rather on the ultrastructural level. This study supports the notion that mitochondrial HMGCS2 is regulated by the fat content of the diet and is involved in the expression of monosaccharide transporters.
    MeSH term(s) Carbohydrates ; Cross-Over Studies ; Glucose ; Humans ; Intestinal Mucosa/pathology ; Jejunum ; Monosaccharides
    Chemical Substances Carbohydrates ; Monosaccharides ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2022-10-04
    Publishing country Switzerland
    Document type Journal Article ; Randomized Controlled Trial
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu14194123
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