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Article ; Online: Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil.

Mascarenhas, Joana D P / Bezerra, Delana A M / Silva, René R / Silva, Mayara J M / Júnior, Edivaldo C Sousa / Soares, Luana S

Archives of virology

2016 Volume 161, Issue 8, Page(s) 2263–2268

Abstract: The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens ...

Abstract	The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
MeSH term(s)	Animals ; Antigens, Viral/genetics ; Antigens, Viral/metabolism ; Brazil ; Capsid Proteins/genetics ; Capsid Proteins/metabolism ; Chickens ; Feces/virology ; Phylogeny ; Poultry Diseases/virology ; Rotavirus/classification ; Rotavirus/genetics ; Rotavirus/isolation & purification ; Rotavirus/metabolism ; Rotavirus Infections/veterinary ; Rotavirus Infections/virology
Chemical Substances	Antigens, Viral ; Capsid Proteins ; VP6 protein, Rotavirus
Language	English
Publishing date	2016-08
Publishing country	Austria
Document type	Journal Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-016-2882-z
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Article: Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

Mascarenhas, Joana D. P / Delana A. M. Bezerra / RenÃ© R. Silva / Mayara J. M. Silva / Edivaldo C. Sousa JÃºnior / Luana S. Soares

Archives of virology. 2016 Aug., v. 161, no. 8

2016

Abstract: The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens ...

Abstract	The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4Â % (8/85) of the pools contained RVF, whereas 10.6Â % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7Â % and 96.8-96.9Â % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
Keywords	Rotavirus ; amino acid sequences ; broiler chickens ; feces ; flocks ; genes ; polyacrylamide gel electrophoresis ; prototypes ; reverse transcriptase polymerase chain reaction ; transcription (genetics) ; Amazonia ; Brazil ; Germany
Language	English
Dates of publication	2016-08
Size	p. 2263-2268.
Publishing place	Springer Vienna
Document type	Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-016-2882-z
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Article ; Online: Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil.

Araujo, Natalia M / Araujo, Oscar C / Silva, Edinete M / Villela-Nogueira, Cristiane A / Nabuco, Letícia C / Parana, Raymundo / Bessone, Fernando / Gomes, Selma A / Trepo, Christian / Kay, Alan

The Journal of general virology

2012 Volume 94, Issue Pt 1, Page(s) 150–158

Abstract: ... in Argentina and co-circulation of HBV/G, HBV/F and G/F recombinants in the American continent. HBV isolates ... Hepatitis B virus (HBV) genotype G (HBV/G) infection is almost always detected along ... human immunodeficiency virus (HIV)-positive patients from Argentina and Brazil, the first report of HBV/G infection ...

Abstract	Hepatitis B virus (HBV) genotype G (HBV/G) infection is almost always detected along with a co-infecting HBV strain that can supply HBeAg, typically HBV/A2. In this study we describe, in two human immunodeficiency virus (HIV)-positive patients from Argentina and Brazil, the first report of HBV/G infection in Argentina and co-circulation of HBV/G, HBV/F and G/F recombinants in the American continent. HBV isolates carrying the 36 bp insertion of HBV/G were the most prevalent in both patients, with >99 % of colonies hybridizing to a probe specific for this insertion. Phylogenetic analyses of full-length genomes and precore/core fragments revealed that F4 and F1b were the co-infecting subgenotypes in the Brazilian and Argentinian patients, respectively. Bootscanning analysis provided evidence of recombination in several clones from both patients, with recombination breakpoints located mainly at the precore/core region. These data should encourage further investigations on the clinical implications of HBV/G recombinants in HBV/HIV co-infected patients.
MeSH term(s)	Amino Acid Sequence ; Argentina ; Base Sequence ; Brazil ; Coinfection/immunology ; Coinfection/virology ; Genome, Viral ; Genotype ; HIV/genetics ; HIV/immunology ; HIV Infections/immunology ; HIV Infections/virology ; Hepatitis B/genetics ; Hepatitis B/immunology ; Hepatitis B/virology ; Hepatitis B virus/genetics ; Hepatitis B virus/immunology ; Humans ; Male ; Molecular Sequence Data ; Phylogeny
Language	English
Publishing date	2012-10-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	219316-4
ISSN	1465-2099 ; 0022-1317
ISSN (online)	1465-2099
ISSN	0022-1317
DOI	10.1099/vir.0.047324-0
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Article: Evaluation of antibacterial activity and phytochemical propsection of Solanum paniculatum Lam. and Operculina hamiltonii (G. Don) D.F. Austin and Staples from the semi-arid region of Paraíba state, Brazil

Lobo, K.M.S. / Athayde, A.C.R. / Silva, A.M.A. / Rodrigues, F.F.G. / Lobo, I.S. / Bezerra, D.A.C. / Costa, J.G.M.

Revista Brasileira de Plantas Medicinais (Brazil)

(May 2010) Volume v. 12, Issue (2), Page(s) p. 227–233

Keywords	SHEEP ; DRUG PLANTS ; DRUGS ; HELMINTHOSES ; OVIN ; PLANTE MEDICINALE ; MEDICAMENT ; HELMINTHOSE ; OVINOS ; PLANTAS MEDICINALES ; MEDICAMENTOS ; HELMINTIASIS ; http://www.fao.org/aos/agrovoc#c_7030 ; http://www.fao.org/aos/agrovoc#c_2393 ; http://www.fao.org/aos/agrovoc#c_2395 ; http://www.fao.org/aos/agrovoc#c_3547
Language	Portuguese
Document type	Article
ISSN	1516-0572
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Article ; Online: Avaliação da atividade antibacteriana e prospecção fitoquímica de Solanum paniculatum Lam. e Operculina hamiltonii (G. Don) D. F. Austin & Staples, do semi-árido paraibano Evaluation of antibacterial activity and phytochemical prospection of Solanum paniculatum Lam. and Operculina hamiltonii (G. Don) D. F. Austin & Staples from the semi-arid region of Paraíba State, Brazil

K.M.S Lôbo / A.C.R Athayde / A.M.A Silva / F.F.G Rodrigues / I.S Lôbo / D.A.C Bezerra / J.G.M Costa

Revista Brasileira de Plantas Medicinais , Vol 12, Iss 2, Pp 227-

2010 Volume 235

Abstract: ... do semi-árido paraibano, Solanum paniculatum Lam. e Operculina hamiltonii (G. Don) D. F. Austin ... Solanum paniculatum Lam. and Operculina hamiltonii (G. Don) D. F. Austin & Staples. Such species have been ...

Abstract	Devido ao aparecimento de populações de parasitos resistentes, as drogas farmacêuticas, alternativas de controle, estão sendo pesquisadas utilizando plantas medicinais. O objetivo deste estudo foi avaliar a atividade antibacteriana e realizar a análise fitoquímica do extrato etanólico de duas espécies do semi-árido paraibano, Solanum paniculatum Lam. e Operculina hamiltonii (G. Don) D. F. Austin & Staples. As referidas espécies são muito utilizadas pela população rural contra verminoses gastrintestinais de ovinos. A prospecção fitoquímica foi obtida a partir dos extratos etanólicos para análise dos constituintes químicos existentes nas duas espécies. As análises dos extratos indicaram a presença de taninos flobabênicos, flavononóis, flavononas e alcalóides em ambas as espécies. A atividade antibacteriana foi determinada inicialmente pelo método de difusão em cavidade. A espécie S. paniculatum apresentou atividade contra Staphylococcus aureus (ATCC 12692), Escherichia coli (ATCC 25922) e Pseudomonas aeruginosa (ATCC 15442), no entanto O. hamintonii não demonstrou atividade contra as linhagens bacterianas testadas. The emergence of resistant parasite populations has led to the study of pharmaceutical drugs, which represent an alternative control. This study aimed to evaluate the antibacterial activity and to perform the phytochemical analysis of the ethanol extract from two species belonging to the semi-arid region of Paraíba State, Brazil: Solanum paniculatum Lam. and Operculina hamiltonii (G. Don) D. F. Austin & Staples. Such species have been largely used by the rural population against ovine gastrointestinal helminthes. Phytochemical prospection was obtained from ethanol extracts for analysis of chemical compounds present in both species. Extract analyses indicated the presence of condensed tannins, flavonols, flavanones, and alkaloids in both species. The antibacterial activity was initially determined by the cavity method. The species S. paniculatum had activity against Staphylococcus aureus (ATCC 12692), Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 15442); however, O. hamintonii did not present activity against the tested bacterial strains.
Keywords	Solanum paniculatum ; Operculina hamiltonii ; extratos etanólicos ; atividade antibacteriana ; ethanol extracts ; antibacterial activity ; Pharmacy and materia medica ; RS1-441 ; Medicine ; R ; DOAJ:Pharmacy and materia medica ; DOAJ:Medicine (General) ; DOAJ:Health Sciences ; Botany ; QK1-989 ; Science ; Q ; DOAJ:Botany ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Language	Portuguese
Publishing date	2010-06-01T00:00:00Z
Publisher	UNESP
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Avaliação da eficácia de taboa (Typha domingensis Pers.) e batata-de-purga [Operculina hamiltonii (G. Don) D.F. Austin & Staples] in natura sobre nematóides gastrintestinais de caprinos, naturalmente infectados, em clima semi-árido Evaluation of the effectiveness of "taboa" (Typha domingensis Pers.) and "batata-de-purga" [Operculina hamiltonii (G. Don) D.F. Austin & Staples] in natura on gastrointestinal nematodes of goats, naturally infected, in the semi-arid region

C.F. Silva / A.C.R. Athayde / W.W. Silva / O.G. Rodrigues / V.L.R. Vilela / P.V.T. Marinho

Revista Brasileira de Plantas Medicinais , Vol 12, Iss 4, Pp 466-

2010 Volume 471

Abstract: ... com Moxidectina 0,2% em dose única de 0,5 mg Kg-1; o Grupo II tratado com Taboa na dose (10 g 20 Kg-1 ... peso vivo); Grupo III com batata-de-purga na dose de 9 g 20 Kg-1 p.v.; Grupo IV tratado com taboa 10 g ... 20 Kg-1 p.v. associada a batata 9 g 20 Kg-1 p.v. durante três dias consecutivos, todos administrados ...

Abstract	Avaliou-se a eficácia da Typha domingensis (taboa) e da Operculina hamiltonii (batata-de-purga) in natura sobre nematóides gastrintestinais de caprinos, naturalmente infectados, em clima semi-árido. Foram utilizados 30 caprinos da raça Moxotó, e o rizoma da taboa e a raiz da batata-de-purga, avaliados através da fitoquímica. As fezes foram coletadas nos dias zero, sete e 25, após, o tratamento da contagem de OPG. Os animais foram distribuídos inteiramente ao acaso, com cinco tratamentos e seis repetições. Grupo I tratado com Moxidectina 0,2% em dose única de 0,5 mg Kg-1; o Grupo II tratado com Taboa na dose (10 g 20 Kg-1 peso vivo); Grupo III com batata-de-purga na dose de 9 g 20 Kg-1 p.v.; Grupo IV tratado com taboa 10 g 20 Kg-1 p.v. associada a batata 9 g 20 Kg-1 p.v. durante três dias consecutivos, todos administrados oralmente e o Grupo V como grupo controle. Os grupos tratados apresentaram redução no número médio de OPG aos 7 e 25 dias pós-tratamento, quando comparado com o grupo controle no respectivo período, sendo a batata-de-purga com maior redução aos 7 e a taboa aos 25 dias. A maior eficácia foi da batata-de-purga de 84% e 70%, nos dias 7 e 25 pós-tratamento. Verificou-se que a batata-de-purga foi eficaz no controle de nematóides gastrintestinais de caprinos no clima semi-árido e que a taboa associada com a batata tem potencial para serem utilizadas em programas alternativos de controle parasitário. The effectiveness of Typha domingensis ("taboa") and Operculina hamiltonii ("batata-de-purga") in natura was evaluated on gastrointestinal nematodes of goats, naturally infected, in the semi-arid region. Thirty Moxotó goats were used, and the rhizome of "taboa" and the root of "batata-de-purga" were analyzed through phytochemistry. Feces were collected on days zero, seven and 25 after the treatment for FECs. The distribution of animals was completely at random, with five treatments and six replicates. Group I was treated with Moxidectin 0.2% in a single dose of 0.5 mg kg-1; Group II was treated with "taboa" at the dose of 10 g 20 Kg-1 body weight; Group III was treated with "batata-de-purga" at the dose of 9 g 20 Kg-1 b.w.; Group IV was treated with "taboa" at 10 g 20 Kg-1 b.w. associated with "batata-de-purga" at 9 g 20 Kg-1 b.w. during three consecutive days. All treatments were orally administered and Group V was used as control. Treated groups showed a reduction in the average number of FECs at seven and 25 days post-treatment when compared to the control group in the respective period; "batata de purga" had a greater reduction at seven days, while "taboa" showed a more pronounced reduction at 25 days. The highest effectiveness was observed for "batata de purga": 84% and 70% at seven and 25 days post treatment. "Batata-de-purga" was effective in controlling gastrointestinal nematodes of goats in the semi-arid climate and "taboa" associated with "batata de purga" have the potential to be used in alternative programs for parasite control.
Keywords	anti-helmínticos ; caprinos ; in natura ; raiz ; rizoma ; anthelmintics ; goats ; root ; rhizome ; Pharmacy and materia medica ; RS1-441 ; Medicine ; R ; DOAJ:Pharmacy and materia medica ; DOAJ:Medicine (General) ; DOAJ:Health Sciences ; Botany ; QK1-989 ; Science ; Q ; DOAJ:Botany ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Language	Portuguese
Publishing date	2010-12-01T00:00:00Z
Publisher	UNESP
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cloning of the transmembrane glycoproteins G and F from a Brazilian isolate of bovine respiratory syncytial virus in a prokaryotic system

L.H.A. Silva / K.C. Cardoso / M.J. Silva / F.R. Spilki / C.W. Arns

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 63, Iss 3, Pp 552-

2011 Volume 558

Abstract: The aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate ...

Abstract	The aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate bovine respiratory syncytial viruses (BRSV) - a Brazilian isolate of BRSV, named BRSV-25-BR in previous studies, in a prokaryotic system to proceed the sequencing of larger genomic fragments. The nucleotide substitutions were confirmed and these clones may also be used in further studies regarding the biological effects of those proteins in vitro and in vivo.
Keywords	vírus respiratório sincicial bovino ; bezerros ; glicoproteínas G e F ; Zoology ; QL1-991 ; Science ; Q ; Animal culture ; SF1-1100 ; Agriculture ; S
Language	English
Publishing date	2011-06-01T00:00:00Z
Publisher	Universidade Federal de Minas Gerais
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: EXTRATOS AQUOSOS DE Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e Cucurbita pepo L. SOBRE OVOS E LARVAS DE HELMINTOS EM Gallus domesticus

Felipe Eduardo Silva Sobral / Patrícia Araújo Brandão / Francisca Inês Sousa Freitas / Ana Célia Andrade Brito / Alysson Kennedy Pereira Souza

Revista Verde de Agroecologia e Desenvolvimento Sustentável, Vol 6, Iss

2011 Volume 1

Abstract: ... in vitro da Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e Cucurbita pepo L. na forma ... positivo utilizou-se mebendazol e para testemunha água destilada estéril. A Operculina hamiltonii (G. DON ... D. F. Austin & Staples (1983) e da Cucurbita pepo L. na forma de extrato aquoso, in vitro ...

Abstract	Na avicultura orgânica, os animais são criados com alimentos saudáveis e isentos de contaminantes. O sistema produtivo de galinhas caipira comumente apresenta infecções maciças por helmintos, o controle se dá pelo uso indiscriminado de anti-helmínticos. Neste contexto, a fitoterapia surge como uma alternativa ecologicamente viável e segura na avicultura moderna. O experimento foi desenvolvido na Granja Menino Jesus de Praga e no Laboratório de Parasitologia Clínica do Centro de Ciências da Saúde da Universidade Federal da Paraíba no município de João Pessoa – PB. Objetivou-se neste trabalho verificar a eficácia anti-helmíntica in vitro da Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e Cucurbita pepo L. na forma de extrato aquoso sobre ovos e larvas de helmintos gastrintestinais de Gallus domesticus. Foram utilizadas 50 galinhas com infecção parasitológica natural, e destas recolhidas fezes para exames laboratoriais. Os extratos foram utilizados nas concentrações 50, 25, 12, 6 e 3 mg/mL-1 para as plantas e como controle positivo utilizou-se mebendazol e para testemunha água destilada estéril. A Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e da Cucurbita pepo L. na forma de extrato aquoso, in vitro, não apresentaram atividade ovicida e larvicida nos helmintos de galinhas caipira.
Keywords	Agriculture (General) ; S1-972 ; Geography. Anthropology. Recreation ; G ; Environmental sciences ; GE1-350
Language	English
Publishing date	2011-06-01T00:00:00Z
Publisher	Grupo Verde de Agroecologia e Abelhas (GVAA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e Cucurbita pepo L. no controle de ovos e larvas de helmintos gastrintestinais de Gallus domesticus

Felipe Eduardo Silva Sobral / Patrícia Araújo Brandão / Francisca Inês Sousa Freitas / Ana Célia Rodrigues Athayde / Alysson Kennedy Pereira Souza

Revista Verde de Agroecologia e Desenvolvimento Sustentável, Vol 5, Iss

2010 Volume 3

Abstract: ... G. DON) D. F. Austin & Staples (1983) e da Cucurbita pepo L. sobre ovos e larvas de helmintos ... e destas recolhidas às fezes para exames. As partes etnobotânicas, o tubérculo da O. hamiltonii (G. DON) D. F. Austin ... tubérculo da O. hamiltonii (G. DON) D. F. Austin & Staples (1983) e da semente da C. pepo L., in vivo ...

Abstract	A avicultura orgânica gera alimentos naturais e com elevado valor nutricionais, contudo este sistema se dá em contato direto com o solo propiciando infecções de nematóides. Na tentativa de minimizar o problema parasitário, vêm sendo empregadas diversas formas de controle, dentre elas o uso de fitoterápicos. O experimento foi realizado no Sítio Menino Jesus de Praga e no Laboratório de Entomologia do Centro de Zoonoses, ambos em João Pessoa – PB. O objetivo foi verificar a eficácia anti-helmíntica in vivo da Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) e da Cucurbita pepo L. sobre ovos e larvas de helmintos gastrintestinais de Gallus domesticus. Foram utilizadas 24 galinhas com infecção parasitológica natural, e destas recolhidas às fezes para exames. As partes etnobotânicas, o tubérculo da O. hamiltonii (G. DON) D. F. Austin & Staples (1983) e as sementes de C. pepo L., foram administrados nas doses de 0,5 g/kg e 2,0 g/kg de peso vivo (PV) respectivamente, o controle positivo com mebendazol na concentração 20,0 mg/mL e na dose de 13,0 mg/kg de PV e o controle negativo com areia “lavada”, na dose de 1,0 g/kg de PV. O emprego do tubérculo da O. hamiltonii (G. DON) D. F. Austin & Staples (1983) e da semente da C. pepo L., in vivo, não apresentaram ação ovicida e larvicida nos helmintos de galinhas caipira.
Keywords	Batata de purga ; Galináceo ; Jerimum ; Verme ; Agriculture (General) ; S1-972 ; Geography. Anthropology. Recreation ; G ; Environmental sciences ; GE1-350
Language	English
Publishing date	2010-05-01T00:00:00Z
Publisher	Grupo Verde de Agroecologia e Abelhas (GVAA)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exuberant endobronchial sarcoidosis - An uncommon form of sarcoidosis.

da Silva, F P / Samouco, G / Fernandes, F

Pulmonology

2024

Language	English
Publishing date	2024-02-02
Publishing country	Spain
Document type	Journal Article
ZDB-ID	3009651-0
ISSN	2531-0437 ; 2531-0429
ISSN (online)	2531-0437
ISSN	2531-0429
DOI	10.1016/j.pulmoe.2024.01.003
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