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  1. Article ; Online: Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.

    Soumier, Amelie / Lio, Guillaume / Demily, Caroline

    Molecular psychiatry

    2024  

    Abstract: Autism spectrum disorder (ASD) is identified by a set of neurodevelopmental divergences that typically affect the social communication domain. ASD is also characterized by heterogeneous cognitive impairments and is associated with cooccurring physical ... ...

    Abstract Autism spectrum disorder (ASD) is identified by a set of neurodevelopmental divergences that typically affect the social communication domain. ASD is also characterized by heterogeneous cognitive impairments and is associated with cooccurring physical and medical conditions. As behaviors emerge as the brain matures, it is particularly essential to identify any gaps in neurodevelopmental trajectories during early perinatal life. Here, we introduce the potential of light-sheet imaging for studying developmental biology and cross-scale interactions among genetic, cellular, molecular and macroscale levels of circuitry and connectivity. We first report the core principles of light-sheet imaging and the recent progress in studying brain development in preclinical animal models and human organoids. We also present studies using light-sheet imaging to understand the development and function of other organs, such as the skin and gastrointestinal tract. We also provide information on the potential of light-sheet imaging in preclinical drug development. Finally, we speculate on the translational benefits of light-sheet imaging for studying individual brain-body interactions in advancing ASD research and creating personalized interventions.
    Language English
    Publishing date 2024-03-05
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1330655-8
    ISSN 1476-5578 ; 1359-4184
    ISSN (online) 1476-5578
    ISSN 1359-4184
    DOI 10.1038/s41380-024-02487-8
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    Zs.A 4812: Show issues Location:
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  2. Article ; Online: An optimized iDISCO+ protocol for tissue clearing and 3D analysis of oxytocin and vasopressin cell network in the developing mouse brain.

    Habart, Marie / Lio, Guillaume / Soumier, Amélie / Demily, Caroline / Sirigu, Angela

    STAR protocols

    2023  Volume 4, Issue 1, Page(s) 101968

    Abstract: ... please refer to Soumier et al. (2021). ...

    Abstract Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to process the 3D cell dataset to analyze cell network using a point-based recording procedure that accurately maps neurons in the Allen brain atlas. This protocol can be applied to any neuronal population, in different brain regions and at different developmental stages. For complete details on the use and execution of this protocol, please refer to Soumier et al. (2021).
    MeSH term(s) Animals ; Mice ; Oxytocin ; Vasopressins ; Brain/diagnostic imaging ; Histological Techniques ; Microscopy
    Chemical Substances Oxytocin (50-56-6) ; Vasopressins (11000-17-2)
    Language English
    Publishing date 2023-01-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101968
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  3. Article ; Online: Oxytocin as a potential defence against Covid-19?

    Soumier, Amélie / Sirigu, Angela

    Medical hypotheses

    2020  Volume 140, Page(s) 109785

    MeSH term(s) Animals ; Antiviral Agents/therapeutic use ; Betacoronavirus/drug effects ; COVID-19 ; Coronavirus Infections/drug therapy ; Humans ; Immunity, Innate/drug effects ; Immunologic Factors/therapeutic use ; Nitric Oxide/metabolism ; Organ Specificity ; Oxidative Stress/drug effects ; Oxytocin/pharmacology ; Oxytocin/physiology ; Oxytocin/therapeutic use ; Pandemics ; Pneumonia, Viral/drug therapy ; Receptors, Oxytocin/physiology ; SARS-CoV-2 ; Vasodilation/drug effects ; Vasodilation/physiology ; Virus Replication/drug effects
    Chemical Substances Antiviral Agents ; Immunologic Factors ; Receptors, Oxytocin ; Nitric Oxide (31C4KY9ESH) ; Oxytocin (50-56-6)
    Keywords covid19
    Language English
    Publishing date 2020-04-23
    Publishing country United States
    Document type Letter
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2020.109785
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    Zs.A 1132: Show issues Location:
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  5. Article ; Online: Oxytocin as a potential defence against Covid-19?

    Soumier, Amélie / Sirigu, Angela

    Medical Hypotheses

    2020  Volume 140, Page(s) 109785

    Keywords General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2020.109785
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  6. Article ; Online: Differential fate between oxytocin and vasopressin cells in the developing mouse brain

    Amelie Soumier / Marie Habart / Guillaume Lio / Caroline Demily / Angela Sirigu

    iScience, Vol 25, Iss 1, Pp 103655- (2022)

    2022  

    Abstract: Summary: Oxytocin (OXT) and arginine vasopressin (AVP), two neuropeptides involved in socio-emotional behaviors have been anatomically defined in the adult brain. Yet their spatial organization during postnatal development is not clearly defined. We ... ...

    Abstract Summary: Oxytocin (OXT) and arginine vasopressin (AVP), two neuropeptides involved in socio-emotional behaviors have been anatomically defined in the adult brain. Yet their spatial organization during postnatal development is not clearly defined. We built a developmental atlas using 3D imaging of cleared immunolabeled tissue over four early postnatal (P) stages, from birth (P0, P3, P7, P14) to young adulthood (≥P56). Our atlas-based mapping revealed that the number of OXT neurons doubles according to unique temporal dynamics in selective hypothalamic regions, namely, the periventricular and paraventricular nuclei, and in a novel location we named the antero-lateral preoptic. In the paraventricular nucleus, single-cell densities and fluorescence analysis demonstrated selective expansion of OXT cells in the antero-ventral division, whereas the postero-dorsal division contained cells present at birth. No changes were observed for AVP neurons. Our findings show the coexisting of innate and plastic OXT/AVP brain circuits probably triggered by environmental adaptation of the social brain.
    Keywords Optical imaging ; Neuroscience ; Developmental biology ; Science ; Q
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Differential fate between oxytocin and vasopressin cells in the developing mouse brain.

    Soumier, Amelie / Habart, Marie / Lio, Guillaume / Demily, Caroline / Sirigu, Angela

    iScience

    2021  Volume 25, Issue 1, Page(s) 103655

    Abstract: Oxytocin (OXT) and arginine vasopressin (AVP), two neuropeptides involved in socio-emotional behaviors have been anatomically defined in the adult brain. Yet their spatial organization during postnatal development is not clearly defined. We built a ... ...

    Abstract Oxytocin (OXT) and arginine vasopressin (AVP), two neuropeptides involved in socio-emotional behaviors have been anatomically defined in the adult brain. Yet their spatial organization during postnatal development is not clearly defined. We built a developmental atlas using 3D imaging of cleared immunolabeled tissue over four early postnatal (P) stages, from birth (P0, P3, P7, P14) to young adulthood (≥P56). Our atlas-based mapping revealed that the number of OXT neurons doubles according to unique temporal dynamics in selective hypothalamic regions, namely, the periventricular and paraventricular nuclei, and in a novel location we named the antero-lateral preoptic. In the paraventricular nucleus, single-cell densities and fluorescence analysis demonstrated selective expansion of OXT cells in the antero-ventral division, whereas the postero-dorsal division contained cells present at birth. No changes were observed for AVP neurons. Our findings show the coexisting of innate and plastic OXT/AVP brain circuits probably triggered by environmental adaptation of the social brain.
    Language English
    Publishing date 2021-12-18
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2021.103655
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  8. Article ; Online: Territorial blueprint in the hippocampal system.

    Wirth, Sylvia / Soumier, Amelie / Eliava, Marina / Derdikman, Dori / Wagner, Shlomo / Grinevich, Valery / Sirigu, Angela

    Trends in cognitive sciences

    2021  Volume 25, Issue 10, Page(s) 831–842

    Abstract: As we skillfully navigate through familiar places, neural computations of distances and coordinates escape our attention. However, we perceive clearly the division of space into socially meaningful territories. 'My space' versus 'your space' is a ... ...

    Abstract As we skillfully navigate through familiar places, neural computations of distances and coordinates escape our attention. However, we perceive clearly the division of space into socially meaningful territories. 'My space' versus 'your space' is a distinction familiar to all of us. Spatial frontiers are social in nature since they regulate individuals' access to utilities in space depending on hierarchy and affiliation. How does the brain integrate spatial geometry with social territory? We propose that the action of oxytocin (OT) in the entorhinal-hippocampal regions supports this process. Grounded on the functional role of the hypothalamic neuropeptide in the hippocampal system, we show how OT-induced plasticity may bias the geometrical coding of place and grid cells to represent social territories.
    MeSH term(s) Brain ; Hippocampus/physiology ; Humans ; Oxytocin/physiology
    Chemical Substances Oxytocin (50-56-6)
    Language English
    Publishing date 2021-07-16
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2010989-1
    ISSN 1879-307X ; 1364-6613
    ISSN (online) 1879-307X
    ISSN 1364-6613
    DOI 10.1016/j.tics.2021.06.005
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  9. Article ; Online: Opposing effects of acute versus chronic blockade of frontal cortex somatostatin-positive inhibitory neurons on behavioral emotionality in mice.

    Soumier, Amelie / Sibille, Etienne

    Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

    2014  Volume 39, Issue 9, Page(s) 2252–2262

    Abstract: Reduced expression of somatostatin (SST) is reported across chronic brain conditions including major depression and normal aging. SST is a signaling neuropeptide and marker of gamma-amino butyric acid (GABA) neurons, which specifically inhibit pyramidal ... ...

    Abstract Reduced expression of somatostatin (SST) is reported across chronic brain conditions including major depression and normal aging. SST is a signaling neuropeptide and marker of gamma-amino butyric acid (GABA) neurons, which specifically inhibit pyramidal neuron dendrites. Studies in auditory cortex suggest that chronic reduction in dendritic inhibition induces compensatory homeostatic adaptations that oppose the effects of acute inhibition. Whether such mechanisms occur in frontal cortex (FC) and affect behavioral outcome is not known. Here, we used two complementary viral vector strategies to examine the effects of acute vs chronic inhibition of SST-positive neurons on behavioral emotionality in adult mice. SST-IRES-Cre mice were injected in FC (prelimbic/precingulate) with CRE-dependent adeno-associated viral (AAV) vector encoding the engineered Gi/o-coupled human muscarinic M4 designer receptor exclusively activated by a designer drug (DREADD-hM4Di) or a control reporter (AAV-DIO-mCherry) for acute or chronic cellular inhibition. A separate cohort was injected with CRE-dependent AAV vectors expressing diphtheria toxin (DTA) to selectively ablate FC SST neurons. Mice were assessed for anxiety- and depressive-like behaviors (defined as emotionality). Results indicate that acute inhibition of FC SST neurons increased behavioral emotionality, whereas chronic inhibition decreased behavioral emotionality. Furthermore, ablation of FC SST neurons also decreased behavioral emotionality under baseline condition and after chronic stress. Together, our results reveal opposite effects of acute and chronic inhibition of FC SST neurons on behavioral emotionality and suggest the recruitment of homeostatic plasticity mechanisms that have implications for understanding the neurobiology of chronic brain conditions affecting dendritic-targeting inhibitory neurons.
    MeSH term(s) Animals ; Anxiety/physiopathology ; Dependovirus ; Depression/physiopathology ; Diphtheria Toxin/toxicity ; Emotions/drug effects ; Emotions/physiology ; Female ; Frontal Lobe/drug effects ; Frontal Lobe/injuries ; Frontal Lobe/physiology ; Gene Knock-In Techniques ; Genetic Vectors ; Humans ; Male ; Mice, Transgenic ; Neural Inhibition/drug effects ; Neurons/drug effects ; Neurons/physiology ; Receptor, Muscarinic M4/genetics ; Somatostatin/genetics ; Somatostatin/metabolism ; Stress, Psychological/physiopathology ; Time Factors
    Chemical Substances Diphtheria Toxin ; Receptor, Muscarinic M4 ; Somatostatin (51110-01-1)
    Language English
    Publishing date 2014-04-01
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 639471-1
    ISSN 1740-634X ; 0893-133X
    ISSN (online) 1740-634X
    ISSN 0893-133X
    DOI 10.1038/npp.2014.76
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    Zs.A 2379: Show issues Location:
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  10. Article ; Online: New Hippocampal Neurons Mature Rapidly in Response to Ketamine But Are Not Required for Its Acute Antidepressant Effects on Neophagia in Rats.

    Soumier, Amelie / Carter, Rayna M / Schoenfeld, Timothy J / Cameron, Heather A

    eNeuro

    2016  Volume 3, Issue 2

    Abstract: Virtually all antidepressant agents increase the birth of granule neurons in the adult dentate gyrus in rodents, providing a key basis for the neurogenesis hypothesis of antidepressant action. The novel antidepressant ketamine, however, shows ... ...

    Abstract Virtually all antidepressant agents increase the birth of granule neurons in the adult dentate gyrus in rodents, providing a key basis for the neurogenesis hypothesis of antidepressant action. The novel antidepressant ketamine, however, shows antidepressant activity in humans within hours, far too rapid for a mechanism involving neuronal birth. Ketamine could potentially act more rapidly by enhancing maturation of new neurons born weeks earlier. To test this possibility, we assessed the effects of S-ketamine (S-(+)-ketamine hydrochloride) injection on maturation, as well as birth and survival, of new dentate gyrus granule neurons in rats, using the immediate-early gene zif268, proliferating cell nuclear antigen, and BrdU, respectively. We show that S-ketamine has rapid effects on new neurons, increasing the proportion of functionally mature young granule neurons within 2 h. A single injection of S-ketamine also increased cell proliferation and functional maturation, and decreased depressive-like behavior, for at least 4 weeks in rats treated with long-term corticosterone administration (a depression model) and controls. However, the behavioral effects of S-ketamine on neophagia were unaffected by elimination of adult neurogenesis. Together, these results indicate that ketamine has surprisingly rapid and long-lasting effects on the recruitment of young neurons into hippocampal networks, but that ketamine has antidepressant-like effects that are independent of adult neurogenesis.
    MeSH term(s) Animals ; Antidepressive Agents/pharmacology ; Cell Proliferation/drug effects ; Cell Proliferation/genetics ; Cell Survival/drug effects ; Cell Survival/genetics ; Corticosterone/administration & dosage ; Depression/drug therapy ; Depression/pathology ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Excitatory Amino Acid Antagonists/pharmacology ; Food Deprivation ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/genetics ; Glial Fibrillary Acidic Protein/genetics ; Glial Fibrillary Acidic Protein/metabolism ; Hippocampus/cytology ; Hippocampus/drug effects ; Ketamine/pharmacology ; Male ; Neurogenesis/drug effects ; Neurons/drug effects ; Rats ; Rats, Long-Evans ; Rats, Transgenic ; Swimming/psychology ; Time Factors
    Chemical Substances Antidepressive Agents ; Excitatory Amino Acid Antagonists ; Glial Fibrillary Acidic Protein ; Ketamine (690G0D6V8H) ; Corticosterone (W980KJ009P)
    Language English
    Publishing date 2016-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
    ZDB-ID 2800598-3
    ISSN 2373-2822 ; 2373-2822
    ISSN (online) 2373-2822
    ISSN 2373-2822
    DOI 10.1523/ENEURO.0116-15.2016
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