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  1. Article: Contribution des responsables des Cancéropôles au Forum/Débat sur le bilan des actions des Cancéropôles - Répartie à l'analyse proposée par Audrey Vézian.

    Chabannon, Christian / Bougnoux, Philippe / Favre, Gilles / Formstecher, Pierre / Laurent-Puig, Pierre / Oudet, Pierre / Trillet-Lenoir, Véronique

    Medecine sciences : M/S

    2014  Volume 30, Issue 10, Page(s) 925

    Abstract: ... to the article authored by Audrey Vézian, and -provide an alternative and more supportive view of the initiatives ...

    Title translation Reply to « Why is it difficult to implement biomedical policy? The ase of the Cancéropôles, the French cancer organizations », by Audrey Vézian, Med Sci (Paris) 2014 Aug-Sep; 30 (8-9) : 803-7.
    Abstract The authors, all in charge of the administration of one of the 7 French Cancéropôles, reply to the article authored by Audrey Vézian, and -provide an alternative and more supportive view of the initiatives -sponsored by these regional cancer research networks.
    MeSH term(s) Biomedical Research/organization & administration ; Humans ; Neoplasms ; Public Policy
    Language French
    Publishing date 2014-10
    Publishing country France
    Document type Comment ; English Abstract ; Letter
    ZDB-ID 632733-3
    ISSN 1958-5381 ; 0767-0974
    ISSN (online) 1958-5381
    ISSN 0767-0974
    DOI 10.1051/medsci/20143010022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2872: Show issues Location:
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  2. Article ; Online: Lupus systémique de l’enfant.

    Laurent, Audrey / Belot, Alexandre

    La Revue du praticien

    2023  Volume 73, Issue 7, Page(s) 775–781

    Abstract: CHILDHOOD-ONSET SYSTEMIC LUPUS. Childhood-onset systemic lupus (SL) is a rare multifactorial autoimmune disease belonging to connectivitis. Clinical presentation is very heterogeneous with multi-systemic involvement: skin, joint, renal, hematological, ... ...

    Title translation Childhood-onset systemic lupus.
    Abstract CHILDHOOD-ONSET SYSTEMIC LUPUS. Childhood-onset systemic lupus (SL) is a rare multifactorial autoimmune disease belonging to connectivitis. Clinical presentation is very heterogeneous with multi-systemic involvement: skin, joint, renal, hematological, neuropsychiatric, pulmonary, cardiac among others. Renal involvement is particularly frequent and severe in children and must be checked ou on a regular basis by screening for proteinuria. Lab exams show the production of antibodies directed against the native double-stranded DNA, excessive production of type I interferon, consumption of complement is observed during periods of flare-up. There are rare forms of monogenic SL which must be evocated in case of early-onset, familial cases or when occurring in boys.
    MeSH term(s) Child ; Male ; Humans ; Autoimmune Diseases ; Kidney ; Proteinuria/diagnosis ; Proteinuria/etiology
    Language French
    Publishing date 2023-10-05
    Publishing country France
    Document type English Abstract ; Journal Article
    ZDB-ID 205365-2
    ISSN 2101-017X ; 0035-2640
    ISSN (online) 2101-017X
    ISSN 0035-2640
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.433: Show issues Location:
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  3. Article ; Online: Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost.

    Nayak, Rigolin / Franěk, Roman / Laurent, Audrey / Pšenička, Martin

    BMC biology

    2024  Volume 22, Issue 1, Page(s) 39

    Abstract: Background: Surrogate production by germline stem cell transplantation is a powerful method to produce donor-derived gametes via a host, a practice known as surrogacy. The gametes produced by surrogates are often analysed on the basis of their ... ...

    Abstract Background: Surrogate production by germline stem cell transplantation is a powerful method to produce donor-derived gametes via a host, a practice known as surrogacy. The gametes produced by surrogates are often analysed on the basis of their morphology and species-specific genotyping, which enables conclusion to be drawn about the donor's characteristics. However, in-depth information, such as data on epigenetic changes, is rarely acquired. Germ cells develop in close contact with supporting somatic cells during gametogenesis in vertebrates, and we hypothesize that the recipient's gonadal environment may cause epigenetic changes in produced gametes and progeny. Here, we extensively characterize the DNA methylome of donor-derived sperm and their intergenerational effects in both inter- and intraspecific surrogates.
    Results: We found more than 3000 differentially methylated regions in both the sperm and progeny derived from inter- and intraspecific surrogates. Hypermethylation in the promoter regions of the protocadherin gamma gene in the intraspecific surrogates was found to be associated with germline transmission. On the contrary, gene expression level and the embryonic development of the offspring remained unaffected. We also discovered MAPK/p53 pathway disruption in interspecific surrogates due to promoter hypermethylation and identified that the inefficient removal of meiotic-arrested endogenous germ cells in hybrid gonads led to the production of infertile spermatozoa.
    Conclusions: Donor-derived sperm and progeny from inter- and intraspecific surrogates were more globally hypermethylated than those of the donors. The observed changes in DNA methylation marks in the surrogates had no significant phenotypic effects in the offspring.
    MeSH term(s) Pregnancy ; Animals ; Female ; Male ; Semen ; Germ Cells/metabolism ; Spermatozoa ; DNA Methylation ; Stem Cells
    Language English
    Publishing date 2024-02-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2133020-7
    ISSN 1741-7007 ; 1741-7007
    ISSN (online) 1741-7007
    ISSN 1741-7007
    DOI 10.1186/s12915-024-01842-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Dual Cross-Linked Stimuli-Responsive Alginate-Based Hydrogels.

    Habib, Michel / Berthalon, Steve / Leclercq, Laurent / Tourrette, Audrey / Sharkawi, Tahmer / Blanquer, Sebastien

    Biomacromolecules

    2024  Volume 25, Issue 3, Page(s) 1660–1670

    Abstract: Sodium alginate with different molecular weights (55, 170, and 320 kg ... ...

    Abstract Sodium alginate with different molecular weights (55, 170, and 320 kg mol
    MeSH term(s) Hydrogels/chemistry ; Alginates/chemistry
    Chemical Substances Hydrogels ; Alginates
    Language English
    Publishing date 2024-02-28
    Publishing country United States
    Document type Journal Article
    ISSN 1526-4602
    ISSN (online) 1526-4602
    DOI 10.1021/acs.biomac.3c01201
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Evaluation of Slowfade Diamond as a buffer for STORM microscopy.

    Boukhatem, Hadjer / Durel, Beatrice / Raimbault, Manon / Laurent, Audrey / Olivier, Nicolas

    Biomedical optics express

    2023  Volume 14, Issue 2, Page(s) 550–558

    Abstract: We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really ...

    Abstract We study the potential of the commercial mounting medium Slowfade diamond as a buffer for STORM microscopy. We show that although it does not work with the popular far-red dyes typically used for STORM imaging, such as Alexa Fluor 647, it performs really well with a wide variety of green-excited dyes such as Alexa Fluor 532, Alexa Fluor 555 or CF 568. Moreover, imaging can be performed several months after the samples are mounted in this environment and kept in the fridge, providing a convenient way to preserve samples for STORM imaging, as well as to keep calibration samples, for example for metrology or teaching in particular in imaging facilities.
    Language English
    Publishing date 2023-01-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.473463
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Strengths and limitations of reduced representation bisulfite sequencing (RRBS) in the perspective of DNA methylation analysis in fish: a case-study on rainbow trout spermatozoa.

    El Kamouh, Marina / Brionne, Aurélien / Sayyari, Amin / Lallias, Delphine / Labbé, Catherine / Laurent, Audrey

    Fish physiology and biochemistry

    2024  

    Abstract: DNA methylation in CpG dinucleotides is an important epigenetic mark in fish spermatozoa since it has been shown that some sperm methylome features are transmitted to the offspring. Reduced representation bisulfite sequencing (RRBS) is one genome-scale ... ...

    Abstract DNA methylation in CpG dinucleotides is an important epigenetic mark in fish spermatozoa since it has been shown that some sperm methylome features are transmitted to the offspring. Reduced representation bisulfite sequencing (RRBS) is one genome-scale methods developed to assess DNA methylation at CpG sites. It allows the sequencing of a reduced fraction of the genome expected to be enriched in CpGs. The aim of this study is to characterize the extent of the CpG sites that can be identified in the RRBS-reduced sequenced fraction of rainbow trout spermatozoa, in order to evaluate the potential of RRBS for sperm DNA methylation studies. We observed that RRBS did provide a reduced amount of genomic data, the sum of the CpGs analyzed on 12 males spanning 9% of the total genomic CpGs. CpGs were only slightly enriched in the RRBS data (×1.7 times the sequenced nucleotides), the possible causes being linked to trout genome structure and sequenced fragments size. All genomic functional features were represented in our CpG dataset, with a noticeable enrichment in exons but, strikingly, not in promoters. The number of CpGs shared between biological replicates was low, but this proportion reached workable values from six biological replicates (46% of the analyzed cytosines) on. The choices that are to be made regarding fragment size selection and the options during bioinformatic data processing are discussed. In all, RRBS is a relevant first-approach method to scan the CpG DNA methylation status of spermatozoa along rainbow trout genome, although in a very reduced pattern among biological replicates.
    Language English
    Publishing date 2024-03-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 292907-7
    ISSN 1573-5168 ; 0920-1742
    ISSN (online) 1573-5168
    ISSN 0920-1742
    DOI 10.1007/s10695-024-01326-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 6206: Show issues

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  7. Article ; Online: Prenatal imaging features related to RAC3 pathogenic variant and differential diagnoses.

    Cabet, Sara / Vasiljevic, Alexandre / Putoux, Audrey / Labalme, Audrey / Sanlaville, Damien / Chatron, Nicolas / Lesca, Gaetan / Guibaud, Laurent

    Prenatal diagnosis

    2022  Volume 42, Issue 4, Page(s) 478–481

    MeSH term(s) Diagnosis, Differential ; Humans ; rac GTP-Binding Proteins/metabolism
    Chemical Substances RAC3 protein, human ; rac GTP-Binding Proteins (EC 3.6.5.2)
    Language English
    Publishing date 2022-02-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 82031-3
    ISSN 1097-0223 ; 0197-3851
    ISSN (online) 1097-0223
    ISSN 0197-3851
    DOI 10.1002/pd.6106
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1625: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article ; Online: Chitosan-PNIPAM Thermogel Associated with Hydrogel Microspheres as a Smart Formulation for MSC Injection.

    Conzatti, Guillaume / Nadal, Clémence / Berthelot, Jade / Vachoud, Laurent / Labour, Marie-Noëlle / Tourrette, Audrey / Belamie, Emmanuel

    ACS applied bio materials

    2024  

    Abstract: Regenerative medicine based on cell therapy has emerged as a promising approach for the treatment of various medical conditions. However, the success of cell therapy heavily relies on the development of suitable injectable hydrogels that can encapsulate ... ...

    Abstract Regenerative medicine based on cell therapy has emerged as a promising approach for the treatment of various medical conditions. However, the success of cell therapy heavily relies on the development of suitable injectable hydrogels that can encapsulate cells and provide a conducive environment for their survival, proliferation, and tissue regeneration. Herein, we address the medical need for cyto- and biocompatible injectable hydrogels by reporting on the synthesis of a hydrogel-forming thermosensitive copolymer. The copolymer was synthesized by grafting poly(
    Language English
    Publishing date 2024-04-08
    Publishing country United States
    Document type Journal Article
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.4c00071
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa.

    El Kamouh, Marina / Brionne, Aurélien / Sayyari, Amin / Laurent, Audrey / Labbé, Catherine

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 19029

    Abstract: Spermatozoa are the cells that are most commonly used for cryopreservation of valuable genetic resources in aquaculture. It is known that fish spermatozoa transmit to the embryo not only their genetic but also their epigenetic profile, especially DNA ... ...

    Abstract Spermatozoa are the cells that are most commonly used for cryopreservation of valuable genetic resources in aquaculture. It is known that fish spermatozoa transmit to the embryo not only their genetic but also their epigenetic profile, especially DNA methylation. Therefore, any alteration of the DNA methylation profile in spermatozoa induces the risk of transmitting epigenetic alterations to the offspring. The aim of this study was to assess the effect of cryopreservation on DNA methylation in rainbow trout spermatozoa. To trigger variable cellular response after freezing-thawing, spermatozoa from mature males were cryopreserved with dimethyl sulfoxide, methanol or glycerol as cryoprotectant. We observed that dimethyl sulfoxide was the best to preserve thawed spermatozoa functions. Methanol only slightly preserved all the cellular parameters, while glycerol failed to protect motility and fertilization ability. The consequences on DNA methylation were assessed using Reduced Representation Bisulfite Sequencing (RRBS). Sperm cryopreservation did not thoroughly impact DNA methylation, although 335-564 differentially methylated cytosines were characterized depending on the cryoprotectant. Very few of them were shared between cryoprotectants, and no correlation with the extent of cellular damage was found. Our study showed that DNA methylation was only slightly altered after sperm cryopreservation, and this may render further analysis of the risk for the progeny very challenging.
    MeSH term(s) Animals ; Male ; Dimethyl Sulfoxide/pharmacology ; Oncorhynchus mykiss/genetics ; Glycerol/pharmacology ; Methanol/pharmacology ; DNA Methylation ; Semen ; Sperm Motility/physiology ; Semen Preservation ; Spermatozoa/physiology ; Cryopreservation ; Cryoprotective Agents/pharmacology
    Chemical Substances Dimethyl Sulfoxide (YOW8V9698H) ; Glycerol (PDC6A3C0OX) ; Methanol (Y4S76JWI15) ; Cryoprotective Agents
    Language English
    Publishing date 2023-11-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-44803-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Spermatozoa methylome and its sensitivity to water temperature in a teleost fish.

    Brionne, Aurélien / Goupil, Anne-Sophie / Kica, Stéphanie / Lareyre, Jean-Jacques / Labbé, Catherine / Laurent, Audrey

    The Science of the total environment

    2023  Volume 892, Page(s) 164077

    Abstract: Global climate change and heat waves are sources of stress which fish are facing in the wild as well as in aquaculture context. In coping with important environmental variations, they demonstrate a great plasticity and a tendency for acclimation ... ...

    Abstract Global climate change and heat waves are sources of stress which fish are facing in the wild as well as in aquaculture context. In coping with important environmental variations, they demonstrate a great plasticity and a tendency for acclimation throughout generations. Here, we question whether fish might be prone to transmit epigenetic alterations through their gametes to their offspring, thus driving rapid environmental adaptation. The question of epigenetic inheritance in fish has become of crucial interest in the recent years, when the mammalian model of methylome erasure in germ cells and embryos was found not to be conserved. In this work, by sequencing spermatozoa after bisulfite conversion, we characterized the methylation landscape of the paternal gamete in rainbow trout (in comparison to muscle) before to demonstrate its sensitivity to a 4 °C increased rearing temperature during spermatogenesis. We found that spermatozoa methylome specifically primes housekeeping and developmental genes for activation and might be instrumental to early development. Most of these methylation-free promoters were not affected by temperature, attesting the robustness of the epigenetic programming of early development. However, the increase of temperature triggered the differential methylation of 5359 regions, among which 560 gene promoters control spermiogenesis and lipid metabolism. We therefore report, for the first time in fish, that sperm epigenetic landscape carries marks of parental thermal living conditions, suggesting that DNA methylation might be a molecular basis of intergenerational inheritance.
    MeSH term(s) Animals ; Male ; Epigenome ; Epigenesis, Genetic ; Temperature ; Semen ; Spermatozoa/physiology ; DNA Methylation ; Mammals
    Language English
    Publishing date 2023-05-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.164077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 949: Show issues Location:
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