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  1. Article ; Online: Palliative care needs of patients living with end-stage kidney disease not treated with renal replacement therapy: An exploratory qualitative study from Blantyre, Malawi.

    Bates, Maya J / Chitani, Alex / Dreyer, Gavin

    African journal of primary health care & family medicine

    2017  Volume 9, Issue 1, Page(s) e1–e6

    Abstract: Background: The burden of end-stage kidney disease (ESKD) in sub-Saharan Africa is increasing rapidly but the palliative care needs of patients living with ESKD are not well described. Resource limitations at both health system and patient level act as ... ...

    Abstract Background: The burden of end-stage kidney disease (ESKD) in sub-Saharan Africa is increasing rapidly but the palliative care needs of patients living with ESKD are not well described. Resource limitations at both health system and patient level act as major barriers to patients receiving renal replacement therapy (RRT) in the form of dialysis. We undertook an exploratory qualitative study to describe the palliative care needs of patients with ESKD who were not receiving RRT, at a government teaching hospital in Blantyre, Malawi.
    Methods: A qualitative, explorative and descriptive design was used. Study participants were adults aged > 18 years with an estimated glomerular filtration rate < 15 ml/min on two separate occasions, three months apart, who either chose not to have or were not deemed suitable for RRT. Data were collected by means of semi-structured interviews.
    Results: In October and November 2013, interviews were conducted with 10 adults (7 women with median age of 60.5 years). All were hypertensive and four were on treatment for HIV. Four themes emerged from the data: changes in functional status because of physical symptoms, financial challenges impacting hospital care, loss of role within the family and the importance of spiritual and cultural beliefs.
    Conclusion: This study reports on four thematic areas which warrant further quantitative and qualitative studies both in Malawi and other low-resource settings, where a growing number of patients with ESKD unable to access RRT will require palliative care in the coming years.
    MeSH term(s) Adult ; Africa South of the Sahara ; Aged ; Female ; Health Services Needs and Demand ; Humans ; Kidney Failure, Chronic/psychology ; Kidney Failure, Chronic/therapy ; Malawi ; Male ; Middle Aged ; Palliative Care/psychology ; Qualitative Research ; Renal Replacement Therapy/psychology
    Language English
    Publishing date 2017-05-29
    Publishing country South Africa
    Document type Journal Article
    ZDB-ID 2526836-3
    ISSN 2071-2936 ; 2071-2928
    ISSN (online) 2071-2936
    ISSN 2071-2928
    DOI 10.4102/phcfm.v9i1.1376
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Palliative care needs of patients living with end-stage kidney disease not treated with renal replacement therapy

    Maya J. Bates / Alex Chitani / Gavin Dreyer

    African Journal of Primary Health Care & Family Medicine, Vol 9, Iss 1, Pp e1-e

    An exploratory qualitative study from Blantyre, Malawi

    2017  Volume 6

    Abstract: Background: The burden of end-stage kidney disease (ESKD) in sub-Saharan Africa is increasing rapidly but the palliative care needs of patients living with ESKD are not well described. Resource limitations at both health system and patient level act as ... ...

    Abstract Background: The burden of end-stage kidney disease (ESKD) in sub-Saharan Africa is increasing rapidly but the palliative care needs of patients living with ESKD are not well described. Resource limitations at both health system and patient level act as major barriers to patients receiving renal replacement therapy (RRT) in the form of dialysis. We undertook an exploratory qualitative study to describe the palliative care needs of patients with ESKD who were not receiving RRT, at a government teaching hospital in Blantyre, Malawi. Methods: A qualitative, explorative and descriptive design was used. Study participants were adults aged > 18 years with an estimated glomerular filtration rate < 15 ml/min on two separate occasions, three months apart, who either chose not to have or were not deemed suitable for RRT. Data were collected by means of semi-structured interviews. Results: In October and November 2013, interviews were conducted with 10 adults (7 women with median age of 60.5 years). All were hypertensive and four were on treatment for HIV. Four themes emerged from the data: changes in functional status because of physical symptoms, financial challenges impacting hospital care, loss of role within the family and the importance of spiritual and cultural beliefs. Conclusion: This study reports on four thematic areas which warrant further quantitative and qualitative studies both in Malawi and other low-resource settings, where a growing number of patients with ESKD unable to access RRT will require palliative care in the coming years.
    Keywords palliative care ; end stage kidney disease ; Malawi ; Medicine ; R ; Public aspects of medicine ; RA1-1270
    Subject code 610
    Language English
    Publishing date 2017-05-01T00:00:00Z
    Publisher AOSIS
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Endocytic Profiling of Cancer Cell Models Reveals Critical Factors Influencing LNP-Mediated mRNA Delivery and Protein Expression.

    Sayers, Edward J / Peel, Samantha E / Schantz, Anna / England, Richard M / Beano, Maya / Bates, Stephanie M / Desai, Arpan S / Puri, Sanyogitta / Ashford, Marianne B / Jones, Arwyn T

    Molecular therapy : the journal of the American Society of Gene Therapy

    2019  Volume 27, Issue 11, Page(s) 1950–1962

    Abstract: Lipid nanoparticles have great potential for delivering nucleic-acid-based therapeutics, but low efficiency limits their broad clinical translation. Differences in transfection capacity between in vitro models used for nanoparticle pre-clinical testing ... ...

    Abstract Lipid nanoparticles have great potential for delivering nucleic-acid-based therapeutics, but low efficiency limits their broad clinical translation. Differences in transfection capacity between in vitro models used for nanoparticle pre-clinical testing are poorly understood. To address this, using a clinically relevant lipid nanoparticle (LNP) delivering mRNA, we highlight specific endosomal characteristics in in vitro tumor models that impact protein expression. A 30-cell line LNP-mRNA transfection screen identified three cell lines having low, medium, and high transfection that correlated with protein expression when they were analyzed in tumor models. Endocytic profiling of these cell lines identified major differences in endolysosomal morphology, localization, endocytic uptake, trafficking, recycling, and endolysosomal pH, identified using a novel pH probe. High-transfecting cells showed rapid LNP uptake and trafficking through an organized endocytic pathway to lysosomes or rapid exocytosis. Low-transfecting cells demonstrated slower endosomal LNP trafficking to lysosomes and defective endocytic organization and acidification. Our data establish that efficient LNP-mRNA transfection relies on an early and narrow endosomal escape window prior to lysosomal sequestration and/or exocytosis. Endocytic profiling should form an important pre-clinical evaluation step for nucleic acid delivery systems to inform model selection and guide delivery-system design for improved clinical translation.
    MeSH term(s) Cell Line, Tumor ; Endocytosis ; Endosomes/metabolism ; Flow Cytometry ; Fluorescent Antibody Technique ; Gene Expression ; Genes, Reporter ; Humans ; Hydrogen-Ion Concentration ; Lipids/chemistry ; Lysosomes/metabolism ; Nanoparticles/chemistry ; RNA, Messenger/administration & dosage ; RNA, Messenger/genetics ; Transfection/methods
    Chemical Substances Lipids ; RNA, Messenger
    Language English
    Publishing date 2019-08-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1016/j.ymthe.2019.07.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Equipping leaders in health in Malawi: Some personal reflections from a leadership skills-building workshop held at the College of Medicine, Blantyre, Malawi.

    Bates, Maya Jane / Gadama, Yohane / Mbamba, Jessie / Manda-Taylor, Lucinda

    Malawi medical journal : the journal of Medical Association of Malawi

    2018  Volume 30, Issue 2, Page(s) 134–136

    MeSH term(s) Humans ; Leadership ; Malawi ; Medicine ; Universities
    Language English
    Publishing date 2018-12-02
    Publishing country Malawi
    Document type Journal Article
    ZDB-ID 2491952-4
    ISSN 1995-7270 ; 1995-7262
    ISSN (online) 1995-7270
    ISSN 1995-7262
    DOI 10.4314/mmj.v30i2.15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Enhanced patient research participation: a Photovoice study in Blantyre Malawi.

    Bates, Maya Jane / Ardrey, Jane / Mphwatiwa, Treza / Squire, Stephen Bertel / Niessen, Louis Willem

    BMJ supportive & palliative care

    2018  Volume 8, Issue 2, Page(s) 171–174

    Abstract: Objectives: Patient involvement in palliative care research is a desirable if challenging goal. Photovoice is an action research method in which affected communities gather photographs to document and discuss their communities' strengths and concerns. ... ...

    Abstract Objectives: Patient involvement in palliative care research is a desirable if challenging goal. Photovoice is an action research method in which affected communities gather photographs to document and discuss their communities' strengths and concerns. Engagement with policymakers is a separately stated goal. Photovoice is increasingly used in health-related research but has not been widely described in the palliative care literature. We report on experiences and lessons learnt using Photovoice in Blantyre, Malawi to encourage its wider use in research and practice.
    Methods: Thirteen co-researchers (six patients and seven household carers, mean age 47 years) receiving community-based palliative care, attended nine half-day group sessions over a 4-month period. Co-researchers produced, selected and analysed photographs. On completion of data collection, they conducted an advocacy event, including a photographic exhibition, to which media representatives and community leaders were invited.
    Results: Procedures to ensure safety of co-researchers and to obtain consent of individuals identified in the photographs were developed during the planning phase. Co-researchers engaged with the Photovoice process with enthusiasm, although frailty and physical disability (poor sight) limited participation for some older adults. Inclusion of palliative care staff within the research team helped to facilitate open dialogue and clinical review where appropriate.
    Conclusions: In this Photovoice study, patients and family members receiving palliative care engaged in an exploration of household well-being using photography, participatory analysis and an advocacy event. With appropriate planning, Photovoice can be adapted to a range of settings to enhance patient participation.
    MeSH term(s) Adult ; Caregivers ; Community-Based Participatory Research/methods ; Community-Based Participatory Research/utilization ; Humans ; Informed Consent ; Malawi ; Middle Aged ; Palliative Care/methods ; Palliative Care/utilization ; Patients ; Photography ; Research Subjects
    Language English
    Publishing date 2018-02-01
    Publishing country England
    Document type Journal Article
    ISSN 2045-4368
    ISSN (online) 2045-4368
    DOI 10.1136/bmjspcare-2017-001439
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: SETD2 loss-of-function promotes renal cancer branched evolution through replication stress and impaired DNA repair.

    Kanu, N / Grönroos, E / Martinez, P / Burrell, R A / Yi Goh, X / Bartkova, J / Maya-Mendoza, A / Mistrík, M / Rowan, A J / Patel, H / Rabinowitz, A / East, P / Wilson, G / Santos, C R / McGranahan, N / Gulati, S / Gerlinger, M / Birkbak, N J / Joshi, T /
    Alexandrov, L B / Stratton, M R / Powles, T / Matthews, N / Bates, P A / Stewart, A / Szallasi, Z / Larkin, J / Bartek, J / Swanton, C

    Oncogene

    2015  Volume 34, Issue 46, Page(s) 5699–5708

    Abstract: Defining mechanisms that generate intratumour heterogeneity and branched evolution may inspire novel therapeutic approaches to limit tumour diversity and adaptation. SETD2 (Su(var), Enhancer of zeste, Trithorax-domain containing 2) trimethylates histone- ... ...

    Abstract Defining mechanisms that generate intratumour heterogeneity and branched evolution may inspire novel therapeutic approaches to limit tumour diversity and adaptation. SETD2 (Su(var), Enhancer of zeste, Trithorax-domain containing 2) trimethylates histone-3 lysine-36 (H3K36me3) at sites of active transcription and is mutated in diverse tumour types, including clear cell renal carcinomas (ccRCCs). Distinct SETD2 mutations have been identified in spatially separated regions in ccRCC, indicative of intratumour heterogeneity. In this study, we have addressed the consequences of SETD2 loss-of-function through an integrated bioinformatics and functional genomics approach. We find that bi-allelic SETD2 aberrations are not associated with microsatellite instability in ccRCC. SETD2 depletion in ccRCC cells revealed aberrant and reduced nucleosome compaction and chromatin association of the key replication proteins minichromosome maintenance complex component (MCM7) and DNA polymerase δ hindering replication fork progression, and failure to load lens epithelium-derived growth factor and the Rad51 homologous recombination repair factor at DNA breaks. Consistent with these data, we observe chromosomal breakpoint locations are biased away from H3K36me3 sites in SETD2 wild-type ccRCCs relative to tumours with bi-allelic SETD2 aberrations and that H3K36me3-negative ccRCCs display elevated DNA damage in vivo. These data suggest a role for SETD2 in maintaining genome integrity through nucleosome stabilization, suppression of replication stress and the coordination of DNA repair.
    MeSH term(s) Carcinoma, Renal Cell/genetics ; Carcinoma, Renal Cell/metabolism ; Cell Line, Tumor ; DNA Repair ; DNA Replication ; Genetic Heterogeneity ; Histone-Lysine N-Methyltransferase/genetics ; Histone-Lysine N-Methyltransferase/metabolism ; Histones/metabolism ; Humans ; Kidney Neoplasms/genetics ; Kidney Neoplasms/metabolism ; Microsatellite Instability ; Mutation ; Nucleosomes/pathology
    Chemical Substances Histones ; Nucleosomes ; Histone-Lysine N-Methyltransferase (EC 2.1.1.43) ; SETD2 protein, human (EC 2.1.1.43)
    Language English
    Publishing date 2015-03-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639046-8
    ISSN 1476-5594 ; 0950-9232
    ISSN (online) 1476-5594
    ISSN 0950-9232
    DOI 10.1038/onc.2015.24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Moringa oleifera as a model system for undergraduate problem-based learning

    Bates, R. M / A. J. Bicksler

    Acta horticulturae. 2017 May 9, , no. 1158

    2017  

    Abstract: The many medicinal, nutritional, industrial, and agricultural uses of Moringa oleifera are well-documented. These unique traits found in moringa also make the species a valuable model system by which undergraduate students from non-agricultural majors ... ...

    Abstract The many medicinal, nutritional, industrial, and agricultural uses of Moringa oleifera are well-documented. These unique traits found in moringa also make the species a valuable model system by which undergraduate students from non-agricultural majors may engage in plant-based research. This paper highlights a collaborative, problem-based learning approach that has been used to a great effect at Penn State University. Problem-based learning promotes a culture of curiosity, where discovery is valued and failure is embraced and used as a learning opportunity. Since 2010, undergraduate moringa-related research projects and activities have included: 1) moringa leaf drying under humid conditions; 2) design of a moringa harvester for application in Senegal; and 3) moringa cationic protein, functionalized sand for water treatment with recognition at the EPA P3 Sustainability Award. Evaluations and anecdotal evidence suggest that collaborative undergraduate research teams engaged around real-world moringa problems experience enhanced critical thinking and problem-solving skills. Additionally, the research conducted by the student teams has contributed significantly to the body of knowledge related to moringa culture and use.
    Keywords college students ; drying ; learning ; leaves ; models ; Moringa oleifera ; problem solving ; research projects ; sand ; teams ; water treatment ; Senegal
    Language English
    Dates of publication 2017-0509
    Size p. 413-416.
    Publishing place International Society for Horticultural Science.
    Document type Article
    Note Paper presented at the I International Symposium on Moringa held November 15-18, 2015, Manila, Philippines
    ISSN 0567-7572
    DOI 10.17660/ActaHortic.2017.1158.47
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: First Report of Laurel Wilt Disease Caused by Raffaelea lauricola on Sassafras in Florida and South Carolina.

    Smith, J A / Dreaden, T J / Mayfield, A E / Boone, A / Fraedrich, S W / Bates, C

    Plant disease

    2019  Volume 93, Issue 10, Page(s) 1079

    Abstract: ... Diego, CA, 1990. (4) J. A. Smith et al. Plant Dis. 93:198, 2009. ...

    Abstract Laurel wilt disease, caused by Raffaelea lauricola (T.C. Harr., Fraedrich & Aghayeva sp. nov.), which is a fungal symbiont of the nonnative redbay ambrosia beetle (Xyleborus glabratus Eichhoff), has caused widespread mortality of native redbay (Persea borbonia (L.) Spreng) in Georgia, South Carolina, and Florida since 2002. The disease has been noted on other species in the Lauraceae including sassafras in Georgia (1), and more recently, on avocado and camphor in Florida (4). Since 2005, wilted shoots, branch dieback, and tree death have been observed in sassafras trees (Sassafras albidum (L.)) in Liberty, McIntosh, Chatham, Effingham, Bulloch, Evans, and Screven counties in Georgia; Bamberg, Beaufort, Charleston, Colleton, Hampton, and Orangeburg counties in South Carolina; and Putnam County in Florida. Symptomatic sassafras trees ranged from 1 to 12 m high and 2.5 to 25 cm in diameter at breast height. In contrast to red bay trees that retain wilted foliage, symptomatic sassafras defoliate rapidly as trees wilt and die. Multiple symptomatic ramets originating from a common root system have been observed. Removal of bark from stem and root sections from wilted trees revealed black-to-brownish staining in the sapwood, characteristic of laurel wilt. Wood chips from symptomatic areas of branches and roots were surface sterilized and plated on cycloheximide-streptomycin malt agar as previously described (1) and R. lauricola was routinely isolated. Small subunit (18S) sequences from rDNA were amplified by PCR and sequenced using primers NS1 and NS4 (3) for isolates from sassafras from Florida and South Carolina. BLASTn searches revealed homology to Raffaelea sp. C2203 (GenBank Accession No. EU123076, 100% similarity) described by Fraedrich et al. (1) from redbay and later named R. lauricola (2). The small subunit rDNA sequences for these isolates have been deposited into GenBank ( http://www.ncbi.nlm.nih.gov/Genbank/index.html ) and assigned Accession Nos. EU980448 (Florida) and GQ329704 (South Carolina). Koch's postulates have been completed with R. lauricola on this host previously (1). Laurel wilt on sassafras often was geographically isolated from other symptomatic hosts in Georgia and South Carolina and appears to occur on this host independently of proximity to redbay. Further studies to determine the epidemiology of laurel wilt on sassafras, potential resistance, and impact on sassafras life history and distribution are needed. Given the clonal nature of sassafras, the disease would appear to have the potential to move through roots of trees once established in a stand. References: (1) S. W Fraedrich et al. Plant Dis. 92:215, 2008. (2) T. C. Harrington et al. Mycotaxon 104:399, 2008. (3) M. A. Innis et al. PCR Protocols, A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990. (4) J. A. Smith et al. Plant Dis. 93:198, 2009.
    Language English
    Publishing date 2019-02-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-93-10-1079B
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A Conceptual Enterprise Framework for Managing Scientific Data Stewardship.

    Peng, Ge / Privette, Jeffrey L / Tilmes, Curt / Bristol, Sky / Maycock, Tom / Bates, John J / Hausman, Scott / Brown, Otis / Kearns, Edward J

    Data science journal

    2018  Volume 17, Page(s) 15

    Abstract: Scientific data stewardship is an important part of long-term preservation and the use/reuse of digital research data. It is critical for ensuring trustworthiness of data, products, and services, which is important for decision-making. Recent U.S. ... ...

    Abstract Scientific data stewardship is an important part of long-term preservation and the use/reuse of digital research data. It is critical for ensuring trustworthiness of data, products, and services, which is important for decision-making. Recent U.S. federal government directives and scientific organization guidelines have levied specific requirements, increasing the need for a more formal approach to ensuring that stewardship activities support compliance verification and reporting. However, many science data centers lack an integrated, systematic, and holistic framework to support such efforts. The current business- and process-oriented stewardship frameworks are too costly and lengthy for most data centers to implement. They often do not explicitly address the federal stewardship requirements and/or the uniqueness of geospatial data. This work proposes a data-centric conceptual enterprise framework for managing stewardship activities, based on the philosophy behind the Plan-Do-Check-Act (PDCA) cycle, a proven industrial concept. This framework, which includes the application of maturity assessment models, allows for quantitative evaluation of how organizations manage their stewardship activities and supports informed decision-making for continual improvement towards full compliance with federal, agency, and user requirements.
    Language English
    Publishing date 2018-06-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2128236-5
    ISSN 1683-1470 ; 1683-1470
    ISSN (online) 1683-1470
    ISSN 1683-1470
    DOI 10.5334/dsj-2018-015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The cost of feeding bred dairy heifers on native warm-season grasses and harvested feedstuffs.

    Lowe, J K / Boyer, C N / Griffith, A P / Waller, J C / Bates, G E / Keyser, P D / Larson, J A / Holcomb, E

    Journal of dairy science

    2016  Volume 99, Issue 1, Page(s) 634–643

    Abstract: Heifer rearing is one of the largest production expenses for dairy cattle operations, which is one reason milking operations outsource heifer rearing to custom developers. The cost of harvested feedstuffs is a major expense in heifer rearing. A possible ... ...

    Abstract Heifer rearing is one of the largest production expenses for dairy cattle operations, which is one reason milking operations outsource heifer rearing to custom developers. The cost of harvested feedstuffs is a major expense in heifer rearing. A possible way to lower feed costs is to graze dairy heifers, but little research exists on this topic in the mid-south United States. The objectives of this research were to determine the cost of feeding bred dairy heifers grazing native warm-season grasses (NWSG), with and without legumes, and compare the cost of grazing with the cost of rearing heifers using 3 traditional rations. The 3 rations were corn silage with soybean meal, corn silage with dry distillers grain, and a wet distillers grain-based ration. Bred Holstein heifers between 15- and 20-mo-old continuously grazed switchgrass (SG), SG with red clover (SG+RC), a big bluestem and Indiangrass mixture (BBIG), and BBIG with red clover (BBIG+RC) in Tennessee during the summer months. Total grazing days were calculated for each NWSG to determine the average cost/animal per grazing day. The average daily gain (ADG) was calculated for each NWSG to develop 3 harvested feed rations that would result in the same ADG over the same number of grazing day as each NWSG treatment. The average cost/animal per grazing day was lowest for SG ($0.48/animal/grazing d) and highest for BBIG+RC ($1.10/animal/grazing d). For both BBIG and SG, legumes increased the average cost/animal per grazing day because grazing days did not increase enough to account for the additional cost of the legumes. No difference was observed in ADG for heifers grazing BBIG (0.85 kg/d) and BBIG+RC (0.94 kg/d), and no difference was observed in ADG for heifers grazing SG (0.71 kg/d) and SG+RC (0.70 kg/d). However, the ADG for heifers grazing SG and SG+RC was lower than the ADG for heifers grazing either BBIG or BBIG+RC. The average cost/animal per grazing day was lower for all NWSG treatments than the average cost/animal per day for all comparable feed rations at a low, average, and high yardage fee. Results of this study suggest that SG was the most cost-effective NWSG alternative to harvested feeds for bred dairy heifer rearing.
    MeSH term(s) Animals ; Breeding ; Cattle/physiology ; Costs and Cost Analysis ; Dairying/economics ; Fabaceae ; Female ; Panicum ; Silage/economics ; Tennessee ; Trifolium ; Zea mays
    Language English
    Publishing date 2016-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2015-9475
    Database MEDical Literature Analysis and Retrieval System OnLINE

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