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  1. Article ; Online: Implant-Based Breast Reconstruction in the Elderly: Complications and Patient-Reported Outcomes in Women Older Than 70 Years.

    Danko, Dora / Ash, Makenna E / Brown, Owen H / Losken, Albert / Thompson, Peter W

    Annals of plastic surgery

    2023  Volume 91, Issue 1, Page(s) 55–61

    Abstract: Background: Advanced age is considered by many to be a relative contraindication to breast reconstruction. However, despite increased medical comorbidities and a perception that elderly patients are less concerned with body image, more women older than ... ...

    Abstract Background: Advanced age is considered by many to be a relative contraindication to breast reconstruction. However, despite increased medical comorbidities and a perception that elderly patients are less concerned with body image, more women older than 70 years are choosing to undergo breast reconstruction. There is a paucity of data to guide reconstructive decision-making and counseling in this population.
    Objectives: The aim of this study was to evaluate patient satisfaction, complication rates, and long-term outcomes in women older than 70 years undergoing implant-based breast reconstruction.
    Methods: A total of 400 patients were identified at the authors' institution and divided into 2 groups: ≥70 and <70 years old. Medical comorbidities, surgical outcomes, and patient-reported outcomes as defined by the BREAST-Q were compared using the χ2 tests for categorical variables and t tests for continuous variables.
    Results: The cohort of patients older than 70 years was made up of 25 women, with a mean age of 73 years, and the cohort of patients younger than 70 years was made up of 375 women, with a mean age of 50 years. There was no significant difference in body mass index (P = 0.373), smoking status (P = 0.360), or history of prior ipsilateral radiation (P = 0.508) between the 2 cohorts; however, the elderly cohort was significantly more likely to have diabetes (P = 0.026). Although elderly patients were less likely to undergo bilateral mastectomy (P < 0.001), there was no significant difference in the type of mastectomy, pathological diagnosis, or method of reconstruction. There was no significant difference in complication rates when looking at minor infection (P = 0.553) or major infection (P = 0.553). The 2 groups were equally likely to undergo secondary procedures (P = 0.192). Overall satisfaction rates were high in all BREAST-Q categories in the elderly group and not significantly different when compared with the group of patients younger than 70 years. Matched-pair analysis showed a significant difference with the group of patients older than 70 years having higher levels physical well-being (P < 0.001).
    Conclusions: Immediate breast reconstruction can be performed safely and with similar high satisfaction rates in the elderly population as their younger counterparts. Age alone should not be used as a reason for excluding women from these life-changing operations.
    MeSH term(s) Humans ; Female ; Aged ; Middle Aged ; Mastectomy/adverse effects ; Breast Neoplasms/surgery ; Breast Neoplasms/complications ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Mammaplasty/methods ; Patient Reported Outcome Measures ; Breast Implants/adverse effects
    Language English
    Publishing date 2023-07-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 423835-7
    ISSN 1536-3708 ; 0148-7043
    ISSN (online) 1536-3708
    ISSN 0148-7043
    DOI 10.1097/SAP.0000000000003615
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Protein Interaction Network Biology in Neuroscience.

    Basu, Avik / Ash, Peter Ea / Wolozin, Benjamin / Emili, Andrew

    Proteomics

    2020  Volume 21, Issue 3-4, Page(s) e1900311

    Abstract: Mapping the intricate networks of cellular proteins in the human brain has the potential to address unsolved questions in molecular neuroscience, including the molecular basis of cognition, synaptic plasticity, long-term potentiation, learning, and ... ...

    Abstract Mapping the intricate networks of cellular proteins in the human brain has the potential to address unsolved questions in molecular neuroscience, including the molecular basis of cognition, synaptic plasticity, long-term potentiation, learning, and memory. Perturbations to the protein-protein interaction networks (PPIN) present in neurons, glia, and other cell-types have been linked to multifactorial neurological disorders. Yet while knowledge of brain PPINs is steadily improving, the complexity and dynamic nature of the heterogeneous central nervous system in normal and disease contexts poses a formidable experimental challenge. In this review, the recent applications of functional proteomics and systems biology approaches to study PPINs central to normal neuronal function, during neurodevelopment, and in neurodegenerative disorders are summarized. How systematic PPIN analysis offers a unique mechanistic framework to explore intra- and inter-cellular functional modules governing neuronal activity and brain function is also discussed. Finally, future technological advancements needed to address outstanding questions facing neuroscience are outlined.
    MeSH term(s) Humans ; Neuroglia ; Neuronal Plasticity ; Neurons ; Protein Interaction Maps ; Systems Biology
    Language English
    Publishing date 2020-12-29
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2032093-0
    ISSN 1615-9861 ; 1615-9853
    ISSN (online) 1615-9861
    ISSN 1615-9853
    DOI 10.1002/pmic.201900311
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  3. Article ; Online: Colloidal dynamics of emulsion droplets in mouth.

    Colijn, Ivanna / Ash, Anthony / Dufauret, Marie / Lepage, Melissa / Loussert-Fonta, Céline / Leser, Martin E / Wilde, Peter J / Wooster, Tim J

    Journal of colloid and interface science

    2022  Volume 620, Page(s) 153–167

    Abstract: The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion ... ...

    Abstract The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion interaction with the tongue, their relative importance is not well understood. In addition, the physical location of emulsions within the saliva papillae on the tongue is not understood at all. A combination of ex vivo salivary film, and in vivo oral coating experiments were used to determine the importance of different mucoadhesive forces. Mucoadhesion of cationic emulsions was largely driven by electrostatic complexation. SDS-PAGE of the in vivo saliva coating highlighted that mucins were largely responsible for cationic emulsion mucoadhesion. Anionic emulsions were bound via hydrophobic/steric interactions to small salivary proteins typically located away from the mucin anchor points. The physical location and clustering of emulsions relative to the salivary film/papillae was probed via the invention of a fluorescent oral microscope. Cationic emulsions were densely clustered close to the papillae whilst anionic emulsions were suspended in the salivary film above the papillae. Interestingly, non-ionic emulsions were also trapped within the salivary film above the papillae as individual droplets. These findings highlight that whilst electrostatic complexation with saliva is a powerful mucoadhesive force, hydrophobic and steric interactions also act to induce oral retention of emulsions. The differences in physical location and clustering of emulsions within the salivary film hint at the 3D locations of the different salivary proteins driving each mucoadhesive interaction. This novel understanding of emulsion saliva/papillae interactions has potential to aid efficacy of buccal pharmaceutical delivery and the reduction of astringency in plant-based foods.
    MeSH term(s) Emulsions/chemistry ; Mouth ; Mucins/chemistry ; Saliva/chemistry ; Salivary Proteins and Peptides/analysis
    Chemical Substances Emulsions ; Mucins ; Salivary Proteins and Peptides
    Language English
    Publishing date 2022-04-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    DOI 10.1016/j.jcis.2022.03.117
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Proximity labeling reveals dynamic changes in the SQSTM1 protein network.

    Rondón Ortiz, Alejandro N / Zhang, Lushuang / Ash, Peter E A / Basu, Avik / Puri, Sambhavi / van der Spek, Sophie J F / Dorrian, Luke / Emili, Andrew / Wolozin, Benjamin

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Sequestosome1 (SQSTM1) is an autophagy receptor that mediates degradation of intracellular cargo, including protein aggregates, through multiple protein interactions. These interactions form the SQSTM1 protein network that are mediated by SQSTM1 ... ...

    Abstract Sequestosome1 (SQSTM1) is an autophagy receptor that mediates degradation of intracellular cargo, including protein aggregates, through multiple protein interactions. These interactions form the SQSTM1 protein network that are mediated by SQSTM1 functional interaction domains, which include LIR, PB1, UBA and KIR. Despite various attempts to unravel the complexity of the SQSTM1 protein network, our understanding of the relationship of various components in cellular physiology and disease states continues to evolve. To investigate the SQSTM1 protein interaction network, we performed proximity profile labeling by fusing TurboID with the human protein SQSTM1 (TurboID::SQSTM1). This chimeric protein displayed well-established SQSTM1 features including: production of SQSTM1 intracellular bodies, binding to known SQSTM1 interacting partners via defined functional SQSTM1 interacting domains and capture of novel SQSTM1 interactors. Strikingly, aggregated tau protein altered the protein interaction network of SQSTM1 to include many stress-associated proteins. Overall, our work reveals the dynamic landscape of the SQSTM1 protein network and offers a resource to study SQSTM1 function in cellular physiology and disease state.
    Language English
    Publishing date 2023-12-13
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.12.12.571324
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: 5' UTR variants in the quantitative trait gene Hnrnph1 support reduced 5' UTR usage and hnRNP H protein as a molecular mechanism underlying reduced methamphetamine sensitivity.

    Ruan, Qiu T / Yazdani, Neema / Reed, Eric R / Beierle, Jacob A / Peterson, Lucy P / Luttik, Kimberly P / Szumlinski, Karen K / Johnson, William E / Ash, Peter E A / Wolozin, Benjamin / Bryant, Camron D

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2020  Volume 34, Issue 7, Page(s) 9223–9244

    Abstract: We previously identified a 210 kb region on chromosome 11 (50.37-50.58 Mb, mm10) containing two protein-coding genes (Hnrnph1, Rufy1) that was necessary for reduced methamphetamine-induced locomotor activity in C57BL/6J congenic mice harboring DBA/2J ... ...

    Abstract We previously identified a 210 kb region on chromosome 11 (50.37-50.58 Mb, mm10) containing two protein-coding genes (Hnrnph1, Rufy1) that was necessary for reduced methamphetamine-induced locomotor activity in C57BL/6J congenic mice harboring DBA/2J polymorphisms. Gene editing of a small deletion in the first coding exon supported Hnrnph1 as a quantitative trait gene. We have since shown that Hnrnph1 mutants also exhibit reduced methamphetamine-induced reward, reinforcement, and dopamine release. However, the quantitative trait variants (QTVs) that modulate Hnrnph1 function at the molecular level are not known. Nine single nucleotide polymorphisms and seven indels distinguish C57BL/6J from DBA/2J within Hnrnph1, including four variants within the 5' untranslated region (UTR). Here, we show that a 114 kb introgressed region containing Hnrnph1 and Rufy1 was sufficient to cause a decrease in MA-induced locomotor activity. Gene-level transcriptome analysis of striatal tissue from 114 kb congenics vs Hnrnph1 mutants identified a nearly perfect correlation of fold-change in expression for those differentially expressed genes that were common to both mouse lines, indicating functionally similar effects on the transcriptome and behavior. Exon-level analysis (including noncoding exons) revealed decreased 5' UTR usage of Hnrnph1 and immunoblot analysis identified a corresponding decrease in hnRNP H protein in 114 kb congenic mice. Molecular cloning of the Hnrnph1 5' UTR containing all four variants (but none of them individually) upstream of a reporter induced a decrease in reporter signal in both HEK293 and N2a cells, thus, identifying a set of QTVs underlying molecular regulation of Hnrnph1.
    MeSH term(s) 5' Untranslated Regions ; Animals ; Central Nervous System Stimulants/pharmacology ; Drug Resistance/genetics ; Exons ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; HEK293 Cells ; Heterogeneous-Nuclear Ribonucleoproteins/genetics ; Humans ; Male ; Methamphetamine/pharmacology ; Mice ; Mice, Congenic ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Motor Activity ; Polymorphism, Genetic ; RNA, Messenger
    Chemical Substances 5' Untranslated Regions ; Central Nervous System Stimulants ; Heterogeneous-Nuclear Ribonucleoproteins ; Hnrnph1 protein, mouse ; RNA, Messenger ; Methamphetamine (44RAL3456C)
    Language English
    Publishing date 2020-05-13
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.202000092R
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  6. Article ; Online: De Novo Cost-Effectiveness Model Framework for Nonalcoholic Steatohepatitis-Modeling Approach and Validation.

    Gal, Peter / Feldmajer, Gyorgyi / Augusto, Margarida / Gani, Ray / Hook, Emma / Bullement, Ash / Philips, Zoe / Smith, Inger

    PharmacoEconomics

    2023  Volume 41, Issue 12, Page(s) 1629–1639

    Abstract: Background: Nonalcoholic steatohepatitis (NASH) is a chronic liver disease associated with hepatic morbidity and mortality and extra-hepatic comorbidities. Published NASH cost-effectiveness models (CEMs) are heterogeneous and consistently omit comorbid ... ...

    Abstract Background: Nonalcoholic steatohepatitis (NASH) is a chronic liver disease associated with hepatic morbidity and mortality and extra-hepatic comorbidities. Published NASH cost-effectiveness models (CEMs) are heterogeneous and consistently omit comorbid conditions that frequently co-exist alongside NASH. We aimed to develop a de novo CEM framework that incorporates extra-hepatic disease states and outcomes alongside hepatic components to enable future estimation of the cost-effectiveness of NASH interventions.
    Methods: Patient-level simulation and cohort-level Markov models were implemented in the same framework. Model inputs included fibrosis progression; late-stage liver disease outcomes; comorbidity outcomes for cardiovascular disease, type 2 diabetes, and obesity; mortality; health-related quality of life; and direct medical costs. The prototype analysis assessed the cost-effectiveness of obeticholic acid versus standard of care from a US payer perspective over a lifetime horizon with costs and effects discounted at 3% per annum. However, the CEM was designed for easy adaptation to other countries, time horizons, and other considerations. Efficacy and adverse event parameters were obtained from the 18-month interim analysis of the REGENERATE trial. Outputs include total and incremental costs, total life years, and quality-adjusted life years.
    Results: In this model, total costs, total life years, and quality-adjusted life years were all higher with obeticholic acid compared with standard of care. Cross-validation of this model with the 2016 and 2020 Institute for Clinical and Economic Review models revealed marked differences, mainly driven by mortality inputs, transition probability estimates, and incorporation of the effect of treatment and comorbidities.
    Conclusion: This is the first CEM in NASH to incorporate the clinical consequences of several comorbidities. The flexible yet standardized framework permits estimation of the cost-effectiveness of NASH interventions in a variety of settings. The model currently includes several assumptions and will be further developed as more relevant data become available.
    MeSH term(s) Humans ; Non-alcoholic Fatty Liver Disease ; Diabetes Mellitus, Type 2/complications ; Quality of Life ; Cost-Benefit Analysis ; Comorbidity
    Language English
    Publishing date 2023-07-28
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 1100273-6
    ISSN 1179-2027 ; 1170-7690
    ISSN (online) 1179-2027
    ISSN 1170-7690
    DOI 10.1007/s40273-023-01298-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: TIA1 potentiates tau phase separation and promotes generation of toxic oligomeric tau.

    Ash, Peter E A / Lei, Shuwen / Shattuck, Jenifer / Boudeau, Samantha / Carlomagno, Yari / Medalla, Maria / Mashimo, Bryce L / Socorro, Guillermo / Al-Mohanna, Louloua F A / Jiang, Lulu / Öztürk, Muhammet M / Knobel, Mark / Ivanov, Pavel / Petrucelli, Leonard / Wegmann, Susanne / Kanaan, Nicholas M / Wolozin, Benjamin

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 9

    Abstract: Tau protein plays an important role in the biology of stress granules and in the stress response of neurons, but the nature of these biochemical interactions is not known. Here we show that the interaction of tau with RNA and the RNA binding protein TIA1 ...

    Abstract Tau protein plays an important role in the biology of stress granules and in the stress response of neurons, but the nature of these biochemical interactions is not known. Here we show that the interaction of tau with RNA and the RNA binding protein TIA1 is sufficient to drive phase separation of tau at physiological concentrations, without the requirement for artificial crowding agents such as polyethylene glycol (PEG). We further show that phase separation of tau in the presence of RNA and TIA1 generates abundant tau oligomers. Prior studies indicate that recombinant tau readily forms oligomers and fibrils in vitro in the presence of polyanionic agents, including RNA, but the resulting tau aggregates are not particularly toxic. We discover that tau oligomers generated during copartitioning with TIA1 are significantly more toxic than tau aggregates generated by incubation with RNA alone or phase-separated tau complexes generated by incubation with artificial crowding agents. This pathway identifies a potentially important source for generation of toxic tau oligomers in tau-related neurodegenerative diseases. Our results also reveal a general principle that phase-separated RBP droplets provide a vehicle for coassortment of selected proteins. Tau selectively copartitions with TIA1 under physiological conditions, emphasizing the importance of TIA1 for tau biology. Other RBPs, such as G3BP1, are able to copartition with tau, but this happens only in the presence of crowding agents. This type of selective mixing might provide a basis through which membraneless organelles bring together functionally relevant proteins to promote particular biological activities.
    MeSH term(s) Amyloid/chemistry ; Amyloid/metabolism ; Humans ; Neurodegenerative Diseases/etiology ; Neurodegenerative Diseases/metabolism ; Neurodegenerative Diseases/pathology ; Neurons/metabolism ; Protein Aggregates ; Protein Aggregation, Pathological ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Multimerization ; RNA Recognition Motif Proteins/chemistry ; RNA Recognition Motif Proteins/metabolism ; Recombinant Proteins ; T-Cell Intracellular Antigen-1/metabolism ; tau Proteins/chemistry ; tau Proteins/metabolism
    Chemical Substances Amyloid ; Protein Aggregates ; RNA Recognition Motif Proteins ; Recombinant Proteins ; T-Cell Intracellular Antigen-1 ; TIA1 protein, human ; tau Proteins
    Language English
    Publishing date 2021-02-22
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2014188118
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  8. Article ; Online: The use of MolecuLight i:X device in acute hand trauma.

    Chew, Bryan J W / Griffin, Michelle / Butler, Peter E / Mosahebi, Ash

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS

    2020  Volume 73, Issue 7, Page(s) 1357–1404

    Abstract: Early diagnosis of wound infections are crucial as they have been shown to increase patient morbidity and mortality. We evaluated the use of Moleculight i:X to identify infections in acute open wounds in hand trauma. Data were collected from patients who ...

    Abstract Early diagnosis of wound infections are crucial as they have been shown to increase patient morbidity and mortality. We evaluated the use of Moleculight i:X to identify infections in acute open wounds in hand trauma. Data were collected from patients who attended the hand trauma unit over a 4 week period prior to having surgery. Wounds were inspected for clinical signs of infection and autofluorescence images were taken using the Moleculight i:X device. Wound swabs were taken and results interpreted according to report by microbiologist. Autofluorescence images were interpreted by a clinician blinded to the microbiology results. 31 patients were included and data collected from 35 wounds. 3 wounds (8.6%) showed positive clinical signs of infection, 3 (8.6%) were positive on autofluorescence imaging and 2 (5.7%) of wound swab samples were positive for significant infection. Autofluorescence imaging correlated with clinical signs and wound swab results for 34 wounds (97.1%). In one case, the clinical assessment and autofluorescence imaging showed positive signs of infection but the wound swabs were negative. Autofluorescence imaging in acute open wounds may be useful to provide real-time confirmation of bacterial infection and therefore guide management.
    MeSH term(s) Hand Injuries/complications ; Hand Injuries/microbiology ; Humans ; Optical Imaging/instrumentation ; Wound Infection/diagnostic imaging ; Wound Infection/etiology
    Language English
    Publishing date 2020-03-16
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2217750-4
    ISSN 1878-0539 ; 1748-6815 ; 0007-1226
    ISSN (online) 1878-0539
    ISSN 1748-6815 ; 0007-1226
    DOI 10.1016/j.bjps.2020.03.004
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  9. Article ; Online: AAPL Practice Resource for the Forensic Psychiatric Evaluation of Competence to Stand Trial.

    Wall, Barry W / Ash, Peter / Keram, Emily / Pinals, Debra A / Thompson, Christopher R

    The journal of the American Academy of Psychiatry and the Law

    2019  Volume 46, Issue 3 Supplement, Page(s) S4–S79

    MeSH term(s) Expert Testimony/legislation & jurisprudence ; Forensic Psychiatry/legislation & jurisprudence ; Humans ; Insanity Defense ; Intellectual Disability/classification ; Mental Competency/legislation & jurisprudence ; Practice Guidelines as Topic/standards ; United States
    Language English
    Publishing date 2019-01-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1381489-8
    ISSN 1943-3662 ; 0091-634X ; 1093-6793
    ISSN (online) 1943-3662
    ISSN 0091-634X ; 1093-6793
    DOI 10.29158/JAAPL.003778-18
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Colloidal dynamics of emulsion droplets in mouth

    Colijn, Ivanna / Ash, Anthony / Dufauret, Marie / lepage, Melissa / Loussert-Fonta, Céline / Leser, Martin E. / Wilde, Peter J. / Wooster, Tim J.

    Journal of Colloid and Interface Science

    2022  Volume 620

    Abstract: The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion ... ...

    Abstract The interaction of emulsions with the tongue is key to the sensory appeal of food and can potentially be exploited for oral/buccal pharmaceutical delivery. Whilst there is good understanding of the different mucoadhesive forces governing emulsion interaction with the tongue, their relative importance is not well understood. In addition, the physical location of emulsions within the saliva papillae on the tongue is not understood at all. A combination of ex vivo salivary film, and in vivo oral coating experiments were used to determine the importance of different mucoadhesive forces. Mucoadhesion of cationic emulsions was largely driven by electrostatic complexation. SDS-PAGE of the in vivo saliva coating highlighted that mucins were largely responsible for cationic emulsion mucoadhesion. Anionic emulsions were bound via hydrophobic/steric interactions to small salivary proteins typically located away from the mucin anchor points. The physical location and clustering of emulsions relative to the salivary film/papillae was probed via the invention of a fluorescent oral microscope. Cationic emulsions were densely clustered close to the papillae whilst anionic emulsions were suspended in the salivary film above the papillae. Interestingly, non-ionic emulsions were also trapped within the salivary film above the papillae as individual droplets. These findings highlight that whilst electrostatic complexation with saliva is a powerful mucoadhesive force, hydrophobic and steric interactions also act to induce oral retention of emulsions. The differences in physical location and clustering of emulsions within the salivary film hint at the 3D locations of the different salivary proteins driving each mucoadhesive interaction. This novel understanding of emulsion saliva/papillae interactions has potential to aid efficacy of buccal pharmaceutical delivery and the reduction of astringency in plant-based foods.
    Keywords Emulsion ; Mucoadhesion ; Oral microscopy ; Papillae ; Saliva ; Salivary film
    Subject code 540
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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