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Article ; Online: Biofilm reactors for the treatment of used water in space:potential, challenges, and future perspectives.

Espinosa-Ortiz, Erika J / Gerlach, Robin / Peyton, Brent M / Roberson, Luke / Yeh, Daniel H

Biofilm

2023 Volume 6, Page(s) 100140

Abstract: Water is not only essential to sustain life on Earth, but also is a crucial resource for long-duration deep space exploration and habitation. Current systems in space rely on the resupply of water from Earth, however, as missions get longer and move ... ...

Abstract	Water is not only essential to sustain life on Earth, but also is a crucial resource for long-duration deep space exploration and habitation. Current systems in space rely on the resupply of water from Earth, however, as missions get longer and move farther away from Earth, resupply will no longer be a sustainable option. Thus, the development of regenerative reclamation water systems through which useable water can be recovered from "waste streams" (
Language	English
Publishing date	2023-07-15
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-2075
ISSN (online)	2590-2075
DOI	10.1016/j.bioflm.2023.100140
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Article ; Online: Interrupted Time Series Analysis of Bar/Tavern Closing Hours and Violent Crime.

Rosen, Erika M / Trangenstein, Pamela J / Fullem, Patrick L / Yeh, Jih-Cheng / Jernigan, David H / Xuan, Ziming

JAMA internal medicine

2024

Abstract: Importance: It is well established that alcohol outlets (ie, places that sell alcohol) attract crime, particularly during late-night hours.: Objective: To evaluate the association of Maryland Senate Bill 571 (SB571), which reduced the hours of sale ... ...

Abstract	Importance: It is well established that alcohol outlets (ie, places that sell alcohol) attract crime, particularly during late-night hours. Objective: To evaluate the association of Maryland Senate Bill 571 (SB571), which reduced the hours of sale for bars/taverns in 1 Baltimore neighborhood from 6 am to 2 am to 9 am to 10 pm, with violent crime within that neighborhood. Design, setting, and participants: This controlled interrupted time series analysis compared the change in violent crime density within an 800-ft buffer around bars/taverns in the treatment neighborhood (ie, subject to SB571) and 2 control areas with a similar mean baseline crime rate, alcohol outlet density, and neighborhood disadvantage score in the City of Baltimore between May 1, 2018, and December 31, 2022. The interrupted time series using Poisson regression with overdispersion adjustment tested whether the violent crime density differed before vs after the policy change in the treatment neighborhood and whether this difference was localized to the treatment neighborhood. Exposure: Statutory reduction of bar/tavern selling hours from 20 to 13 hours per day in the treatment neighborhood. Main outcomes and measures: The primary outcome was all violent crime, including homicide, robbery, aggravated and common assault, and forcible rape. Secondary outcomes were homicides and assaults. All violent crime measures summed the monthly incidents within 800 ft of bars/taverns from 8 pm to 4 am. For each outcome, a level change estimated the immediate change (first month after implementation), and a slope change estimated the sustained change after implementation (percent reduction after the first month). These level and slope changes were then compared between the treatment and control neighborhoods. Results: The treatment neighborhood included 26 bars/taverns (mean [SD] population, 524.6 [234.6] residents), and the control neighborhoods included 41 bars/taverns (mean [SD] population per census block, 570.4 [217.4] residents). There was no immediate level change in density of all violent crimes the month after implementation of SB571; however, compared with the control neighborhoods, the slope of all violent crime density decreased by 23% per year in the treatment neighborhood after SB571 implementation (annualized incidence rate ratio, 0.77; 95% CI, 0.60-0.98; P = .04). Similar results were seen for homicides and assaults. Several sensitivity analyses supported the robustness of these results. Conclusions and relevance: This study's findings suggest that alcohol policies that reduce hours of sale could be associated with a reduction in violent crimes. Given these findings, SB571 may serve as a model for other cities looking to create safer neighborhoods.
Language	English
Publishing date	2024-04-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2699338-7
ISSN	2168-6114 ; 2168-6106
ISSN (online)	2168-6114
ISSN	2168-6106
DOI	10.1001/jamainternmed.2024.0255
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Ua VI Zs.119: Show issues

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Article ; Online: If genetic variation could talk: What genomic data may teach us about the importance of gene expression regulation in the genetics of autism.

Yeh, Erika / Weiss, Lauren A

Molecular and cellular probes

2016 Volume 30, Issue 6, Page(s) 346–356

Abstract: Autism spectrum disorder (ASD) has been long known to have substantial genetic etiology. Much research has attempted to identify specific genes contributing to ASD risk with the goal of tying gene function to a molecular pathological explanation for ASD. ...

Abstract	Autism spectrum disorder (ASD) has been long known to have substantial genetic etiology. Much research has attempted to identify specific genes contributing to ASD risk with the goal of tying gene function to a molecular pathological explanation for ASD. A unifying molecular pathology would potentially increase understanding of what is going wrong during development, and could lead to diagnostic biomarkers or targeted preventative or therapeutic directions. We review past and current genetic mapping approaches and discuss major results, leading to the hypothesis that global dysregulation of gene or protein expression may be implicated in ASD rather than disturbance of brain-specific functions. If substantiated, this hypothesis might indicate the need for novel experimental and analytical approaches in order to understand this neurodevelopmental disorder, develop biomarkers, or consider treatment approaches.
Language	English
Publishing date	2016-12
Publishing country	England
Document type	Review ; Journal Article
ZDB-ID	639082-1
ISSN	1096-1194 ; 0890-8508
ISSN (online)	1096-1194
ISSN	0890-8508
DOI	10.1016/j.mcp.2016.10.007
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Zs.A 2230: Show issues

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Article: If genetic variation could talk: What genomic data may teach us about the importance of gene expression regulation in the genetics of autism

Yeh, Erika / Lauren A. Weiss

Molecular and cellular probes. 2016 Dec., v. 30, no. 6

2016

Abstract	Autism spectrum disorder (ASD) has been long known to have substantial genetic etiology. Much research has attempted to identify specific genes contributing to ASD risk with the goal of tying gene function to a molecular pathological explanation for ASD. A unifying molecular pathology would potentially increase understanding of what is going wrong during development, and could lead to diagnostic biomarkers or targeted preventative or therapeutic directions. We review past and current genetic mapping approaches and discuss major results, leading to the hypothesis that global dysregulation of gene or protein expression may be implicated in ASD rather than disturbance of brain-specific functions. If substantiated, this hypothesis might indicate the need for novel experimental and analytical approaches in order to understand this neurodevelopmental disorder, develop biomarkers, or consider treatment approaches.
Keywords	analytical methods ; autism ; biomarkers ; chromosome mapping ; etiology ; genes ; genetic variation ; protein synthesis ; risk
Language	English
Dates of publication	2016-12
Size	p. 346-356.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	639082-1
ISSN	1096-1194 ; 0890-8508
ISSN (online)	1096-1194
ISSN	0890-8508
DOI	10.1016/j.mcp.2016.10.007
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Article ; Online: Suicide Screening, Risk Assessment, and Lethal Means Counseling During Zero Suicide Implementation.

Boggs, Jennifer M / Richards, Julie / Simon, Gregory / Aguirre-Miyamoto, Erika M / Barton, Lee J / Beck, Arne / Beidas, Rinad S / Bruschke, Cambria / Buckingham, Edward T / Buttlaire, Stuart / Clarke, Gregory / Coleman, Karen / Flores, Jean P / Frank, Catherine / Penfold, Robert B / Richardson, Laura / Ryan, Jacqueline M / Schoenbaum, Michael / Sterling, Stacy /

Stewart, Christine / Yarborough, Bobbi Jo H / Yeh, Hsueh-Han / Ahmedani, Brian

Psychiatric services (Washington, D.C.)

2024 , Page(s) appips20230211

Abstract: Objective: The authors measured implementation of Zero Suicide (ZS) clinical practices that support identification of suicide risk and risk mitigation, including screening, risk assessment, and lethal means counseling, across mental health specialty and ...

Abstract	Objective: The authors measured implementation of Zero Suicide (ZS) clinical practices that support identification of suicide risk and risk mitigation, including screening, risk assessment, and lethal means counseling, across mental health specialty and primary care settings. Methods: Six health care systems in California, Colorado, Michigan, Oregon, and Washington participated. The sample included members ages ≥13 years from 2010 to 2019 (N=7,820,524 patients). The proportions of patients with suicidal ideation screening, suicide risk assessment, and lethal means counseling were estimated. Results: In 2019, patients were screened for suicidal ideation in 27.1% (range 5.0%-85.0%) of mental health visits and 2.5% (range 0.1%-35.0%) of primary care visits among a racially and ethnically diverse sample (44.9% White, 27.2% Hispanic, 13.4% Asian, and 7.7% Black). More patients screened positive for suicidal ideation in the mental health setting (10.2%) than in the primary care setting (3.8%). Of the patients screening positive for suicidal ideation in the mental health setting, 76.8% received a risk assessment, and 82.4% of those identified as being at high risk received lethal means counseling, compared with 43.2% and 82.4%, respectively, in primary care. Conclusions: Six health systems that implemented ZS showed a high level of variation in the proportions of patients receiving suicide screening and risk assessment and lethal means counseling. Two opportunities emerged for further study to increase frequency of these practices: expanding screening beyond patients with regular health care visits and implementing risk assessment with lethal means counseling in the primary care setting directly after a positive suicidal ideation screening.
Language	English
Publishing date	2024-04-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1220173-x
ISSN	1557-9700 ; 1075-2730
ISSN (online)	1557-9700
ISSN	1075-2730
DOI	10.1176/appi.ps.20230211
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Zs.A 416: Show issues

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Article ; Online: Older adults' perspectives 3 months after emergency general surgery highlight opportunities to improve care.

Sokas, Claire / Yeh, Irene M / Bernacki, Rachelle E / Rangel, Erika L / Kaafarani, Haytham / Mitchell, Susan L / Bader, Angela M / Ladin, Keren / Palmer, Jennifer A / Tulsky, James A / Cooper, Zara

Journal of the American Geriatrics Society

2021 Volume 69, Issue 7, Page(s) 2023–2025

MeSH term(s)	Aged ; Aged, 80 and over ; Emergency Service, Hospital/standards ; Female ; Humans ; Male ; Patient Acceptance of Health Care/psychology ; Postoperative Care/psychology ; Postoperative Care/standards ; Postoperative Period ; Qualitative Research ; Quality Improvement ; Surgical Procedures, Operative/rehabilitation
Language	English
Publishing date	2021-04-02
Publishing country	United States
Document type	Letter ; Research Support, N.I.H., Extramural
ZDB-ID	80363-7
ISSN	1532-5415 ; 0002-8614
ISSN (online)	1532-5415
ISSN	0002-8614
DOI	10.1111/jgs.17152
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Ud II Zs.168: Show issues

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Article ; Online: Catecholaminergic Vasopressors Reduce Toll-Like Receptor Agonist-Induced Microvascular Endothelial Cell Permeability But Not Cytokine Production.

Joffre, Jérémie / Lloyd, Elliot / Wong, Erika / Chung-Yeh, Che / Nguyen, Nina / Xu, Fenguyn / Legrand, Matthieu / Hellman, Judith

Critical care medicine

2021 Volume 49, Issue 3, Page(s) e315–e326

Abstract: Objectives: Catecholaminergic vasopressors are the cornerstone of circulatory shock management. Nevertheless, catecholamines have problematic side effects, arousing a growing interest in noncatecholaminergic agents such as vasopressin or angiotensin-II. ...

Abstract	Objectives: Catecholaminergic vasopressors are the cornerstone of circulatory shock management. Nevertheless, catecholamines have problematic side effects, arousing a growing interest in noncatecholaminergic agents such as vasopressin or angiotensin-II. However, their respective effects on sepsis-associated microvascular endothelial dysfunction such as permeability or inflammation remain elusive. We investigated the role of catecholamines and other vasopressors on Toll-like receptor agonists-induced microvascular endothelial permeability and inflammation. Setting: University research laboratory/cell research. Subjects: Human pulmonary microvascular endothelial cells from multiple donors. Intervention: Confluent monolayers of human pulmonary microvascular endothelial cells were treated with Toll-like receptor agonists (lipopolysaccharide, Poly[I:C], or tripalmitoyl-S-glyceryl cysteine) in the presence or absence of epinephrine, norepinephrine, vasopressin, and angiotensin-II. Permeability was inferred from transendothelial resistance, measured using electrical cell impedance sensing, where decreased transendothelial resistance is consistent with increased permeability. Cell-cell junction molecule expression was assessed via immunofluorescence microscopy and flow cytometry. We quantified cytokines in supernatants of Toll-like receptor agonist-treated human pulmonary microvascular endothelial cells. Measurements and main results: Epinephrine and norepinephrine both ameliorate lipopolysaccharide, polyinosinic:polycytidylic acid, or tripalmitoyl-S-glyceryl cysteine-induced reductions in transendothelial resistance, a surrogate for endothelial permeability. In contrast, the noncatecholaminergic agents, vasopressin, and angiotensin-II did not affect Toll-like receptor agonists-induced reductions in transendothelial resistance. β1- and β2-adrenergic receptor antagonists reduced the effects of the catecholamines on transendothelial resistance, whereas α-adrenergic receptor antagonists did not. We observed that epinephrine and norepinephrine induced actin cytoskeletal rearrangement and normalized the membrane expression of proteins involved with adherens-junctions (vascular endothelial-cadherin) and tight-junctions (zona occludens-1). Despite having a substantial effect on endothelial permeability, epinephrine and norepinephrine did not affect human pulmonary microvascular endothelial cell survival or production of interleukin-8, interleukin-6, or monocyte chemoattractant protein-1 (CCL-2) induced by Toll-like receptor agonists, suggesting that these functions are regulated separately from permeability. Conclusions: Our findings demonstrate that treatment with epinephrine or norepinephrine strongly reduces endothelial permeability induced by agonists of multiple Toll-like receptors (Toll-like receptor-2, Toll-like receptor-3, Toll-like receptor-4) in vitro. Our studies suggest that both β1- and β2-adrenergic receptors mediate the stabilizing effects of epinephrine and norepinephrine on the endothelial barrier.
MeSH term(s)	Capillary Permeability/drug effects ; Cell Movement/drug effects ; Cells, Cultured ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Endothelium, Vascular/drug effects ; Epinephrine/pharmacology ; Humans ; Norepinephrine/pharmacology ; Toll-Like Receptors/agonists ; Vasoconstrictor Agents/pharmacology
Chemical Substances	Toll-Like Receptors ; Vasoconstrictor Agents ; Norepinephrine (X4W3ENH1CV) ; Epinephrine (YKH834O4BH)
Language	English
Publishing date	2021-01-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	197890-1
ISSN	1530-0293 ; 0090-3493
ISSN (online)	1530-0293
ISSN	0090-3493
DOI	10.1097/CCM.0000000000004854
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Zs.A 1261: Show issues

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Article ; Online: Quantitative PCR as a marker for preemptive therapy and its role in therapeutic control in Trypanosoma cruzi/HIV coinfection.

Freitas, Vera Lúcia Teixeira de / Novaes, Christina Terra Gallafrio / Sartori, Ana Marli Christovam / Carvalho, Noemia Barbosa / Silva, Sheila Cristina Vicente da / Nakanishi, Érika Shimoda / Salvador, Fernando / Castro, Cleudson Nery de / Bezerra, Rita Cristina / Westphalen, Elizabeth Visone Nunes / Oliveira, Caroline Medeji Ramos de / Busser, Felipe Delatorre / Ho, Yeh-Li / Buccheri, Renata / Bonilla, Carolina / Shikanai-Yasuda, Maria Aparecida

PLoS neglected tropical diseases

2024 Volume 18, Issue 2, Page(s) e0011961

Abstract: Background: Trypanosoma cruzi and HIV coinfection can evolve with depression of cellular immunity and increased parasitemia. We applied quantitative PCR (qPCR) as a marker for preemptive antiparasitic treatment to avoid fatal Chagas disease reactivation ...

Abstract	Background: Trypanosoma cruzi and HIV coinfection can evolve with depression of cellular immunity and increased parasitemia. We applied quantitative PCR (qPCR) as a marker for preemptive antiparasitic treatment to avoid fatal Chagas disease reactivation and analyzed the outcome of treated cases. Methodology: This mixed cross-sectional and longitudinal study included 171 Chagas disease patients, 60 coinfected with HIV. Of these 60 patients, ten showed Chagas disease reactivation, confirmed by parasites identified in the blood, cerebrospinal fluid, or tissues, 12 exhibited high parasitemia without reactivation, and 38 had low parasitemia and no reactivation. Results: We showed, for the first time, the success of the timely introduction of benznidazole in the non-reactivated group with high levels of parasitemia detected by qPCR and the absence of parasites in reactivated cases with at least 58 days of benznidazole. All HIV+ patients with or without reactivation had a 4.0-5.1 higher chance of having parasitemia than HIV seronegative cases. A positive correlation was found between parasites and viral loads. Remarkably, treated T. cruzi/HIV-coinfected patients had 77.3% conversion from positive to negative parasitemia compared to 19.1% of untreated patients. Additionally, untreated patients showed ~13.6 times higher Odds Ratio of having positive parasitemia in the follow-up period compared with treated patients. Treated and untreated patients showed no differences regarding the evolution of Chagas disease. The main factors associated with all-cause mortality were higher parasitemia, lower CD4 counts/μL, higher viral load, and absence of antiretroviral therapy. Conclusion: We recommend qPCR prospective monitoring of T. cruzi parasitemia in HIV+ coinfected patients and point out the value of pre-emptive therapy for those with high parasitemia. In parallel, early antiretroviral therapy introduction is advisable, aiming at viral load control, immune response restoration, and increasing survival. We also suggest an early antiparasitic treatment for all coinfected patients, followed by effectiveness analysis alongside antiretroviral therapy.
MeSH term(s)	Humans ; Trypanosoma cruzi/genetics ; Parasitemia/drug therapy ; Parasitemia/parasitology ; Longitudinal Studies ; Cross-Sectional Studies ; Prospective Studies ; Chagas Disease/complications ; Chagas Disease/drug therapy ; Chagas Disease/parasitology ; Nitroimidazoles/therapeutic use ; HIV Infections/complications ; HIV Infections/drug therapy ; Polymerase Chain Reaction ; Antiparasitic Agents/therapeutic use ; Coinfection/parasitology
Chemical Substances	benzonidazole (YC42NRJ1ZD) ; Nitroimidazoles ; Antiparasitic Agents
Language	English
Publishing date	2024-02-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2735
ISSN (online)	1935-2735
ISSN	1935-2735
DOI	10.1371/journal.pntd.0011961
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Article ; Online: Becoming a Parent During Cardiovascular Training.

Oliveros, Estefania / Burgess, Sonya / Nadella, Neelima / Davidson, Laura / Brailovsky, Yevgeniy / Reza, Nosheen / Squeri, Erika / Mehran, Roxana / DeFaria Yeh, Doreen / Park, Ki

Journal of the American College of Cardiology

2022 Volume 79, Issue 21, Page(s) 2119–2126

Abstract: Background: Specialty training in cardiovascular diseases is consistently perceived to have adverse job conditions and interfere with family life. There is a dearth of universal workforce support for trainees who become parents during training.: ... ...

Abstract	Background: Specialty training in cardiovascular diseases is consistently perceived to have adverse job conditions and interfere with family life. There is a dearth of universal workforce support for trainees who become parents during training. Objectives: This study sought to identify parental policies across cardiovascular training programs internationally. Methods: An Internet-based international survey study available from August 2020 to October 2020 was sent via social media. The survey was administered 1 time and anonymously. Participants shared experiences regarding parental benefits/policies and perception of barriers for trainees. Participants were divided into 3 groups: training program directors, trainees pregnant during cardiology fellowship, and trainees not pregnant during training. Results: A total of 417 replies were received from physicians, including 47 responses (11.3%) from training program directors, 146 responses (35%) from current or former trainees pregnant during cardiology training, and 224 responses (53.7%) from current or former trainees that were not pregnant during cardiology training. Among trainees, 280 (67.1%) were parents during training. Family benefits and policies were not uniformly available across institutions, and knowledge regarding the existence of such policies was low. Average parental leave ranged from 1 to 2 months in the United States compared with >4 months outside the United States, and in all countries, paternity leave was uncommon (only 11 participants [2.6%]). Coverage during family leave was primarily provided by peers (n = 184 [44.1%]), and 168 (91.3%) were without additional monetary or time compensation. Conclusions: This is the first international survey evaluating and comparing parental benefits and policies among cardiovascular training programs. There is great variability among institutions, highlighting disparities in real-world experiences.
MeSH term(s)	Cardiovascular Diseases/therapy ; Fellowships and Scholarships ; Female ; Humans ; Internship and Residency ; Parental Leave ; Parents ; Pregnancy ; United States
Language	English
Publishing date	2022-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	605507-2
ISSN	1558-3597 ; 0735-1097
ISSN (online)	1558-3597
ISSN	0735-1097
DOI	10.1016/j.jacc.2022.03.371
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Zs.A 1846: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Yellow fever disease severity and endothelial dysfunction are associated with elevated serum levels of viral NS1 protein and syndecan-1.

de Sousa, Francielle T G / Warnes, Colin M / Manuli, Erika R / Ng, Arash / D'Elia Zanella, Luiz G F A B / Ho, Yeh-Li / Bhat, Samhita / Romano, Camila M / Beatty, P Robert / Biering, Scott B / Kallas, Esper G / Sabino, Ester C / Harris, Eva

medRxiv : the preprint server for health sciences

2023

Abstract: Yellow fever virus (YFV) infections can cause severe disease manifestations, including hepatic injury, endothelial damage, coagulopathy, hemorrhage, systemic organ failure, and shock, and are associated with high mortality in humans. While nonstructural ... ...

Abstract	Yellow fever virus (YFV) infections can cause severe disease manifestations, including hepatic injury, endothelial damage, coagulopathy, hemorrhage, systemic organ failure, and shock, and are associated with high mortality in humans. While nonstructural protein 1 (NS1) of the related dengue virus is implicated in contributing to vascular leak, little is known about the role of YFV NS1 in severe YF and mechanisms of vascular dysfunction in YFV infections. Here, using serum samples from qRT-PCR-confirmed YF patients with severe (n=39) or non-severe (n=18) disease in a well-defined hospital cohort in Brazil, plus samples from healthy uninfected controls (n=11), we investigated factors associated with disease severity. We developed a quantitative YFV NS1 capture ELISA and found significantly increased levels of NS1, as well as syndecan-1, a marker of vascular leak, in serum from severe YF as compared to non-severe YF or control groups. We also showed that hyperpermeability of endothelial cell monolayers treated with serum from severe YF patients was significantly higher compared to non-severe YF and control groups as measured by transendothelial electrical resistance (TEER). Further, we demonstrated that YFV NS1 induces shedding of syndecan-1 from the surface of human endothelial cells. Notably, YFV NS1 serum levels significantly correlated with syndecan-1 serum levels and TEER values. Syndecan-1 levels also significantly correlated with clinical laboratory parameters of disease severity, viral load, hospitalization, and death. In summary, this study points to a role for secreted NS1 in YF disease severity and provides evidence for endothelial dysfunction as a mechanism of YF pathogenesis in humans.
Language	English
Publishing date	2023-06-30
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.06.29.23292053
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