Article ; Online: Application of digital PCR to determine the reliability of Xpert Xpress SARS-CoV-2 assay with envelope (E) gene negative and nucleocapsid (N2) gene positive results.
Diagnostic microbiology and infectious disease
2022 Volume 103, Issue 4, Page(s) 115726
Abstract: This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR ... ...
Abstract | This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives. |
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MeSH term(s) | COVID-19/diagnosis ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Humans ; Nasopharynx ; Nucleocapsid/genetics ; Polymerase Chain Reaction ; Reproducibility of Results ; SARS-CoV-2/genetics ; Sensitivity and Specificity |
Language | English |
Publishing date | 2022-05-20 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 604920-5 |
ISSN | 1879-0070 ; 0732-8893 |
ISSN (online) | 1879-0070 |
ISSN | 0732-8893 |
DOI | 10.1016/j.diagmicrobio.2022.115726 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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