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  1. Article ; Online: Efficient Recovery of Candida auris and Five Other Medically Important

    Posteraro, Brunella / Menchinelli, Giulia / Ivagnes, Vittorio / Cortazzo, Venere / Liotti, Flora Marzia / Falasca, Benedetta / Fiori, Barbara / D'Inzeo, Tiziana / Spanu, Teresa / De Angelis, Giulia / Sanguinetti, Maurizio

    Microbiology spectrum

    2023  , Page(s) e0410422

    Abstract: Candida auris and ... ...

    Abstract Candida auris and other
    Language English
    Publishing date 2023-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.04104-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Chip-Based Molecular Evaluation of a DNA Extraction Protocol for

    Ivagnes, Vittorio / Menchinelli, Giulia / Liotti, Flora Marzia / De Carolis, Elena / Torelli, Riccardo / De Lorenzis, Desy / Recine, Cinzia / Sanguinetti, Maurizio / D'Inzeo, Tiziana / Posteraro, Brunella

    Microorganisms

    2023  Volume 12, Issue 1

    Abstract: The diagnosis ... ...

    Abstract The diagnosis of
    Language English
    Publishing date 2023-12-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12010081
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Performance of a Novel Real-Time PCR-Based Assay for Rapid Monkeypox Virus Detection in Human Samples.

    Liotti, Flora Marzia / Marchetti, Simona / Falletta, Federico / D'Onghia, Sara / Sanguinetti, Maurizio / Santangelo, Rosaria / Posteraro, Brunella

    Microorganisms

    2023  Volume 11, Issue 10

    Abstract: The ongoing epidemic of mpox, namely human monkeypox virus (MPXV) infection, requires rapid and reliable laboratory diagnosis. We report on the QIAstat-Dx viral vesicular panel PCR assay that allows the detection of (within 75 min) six vesicular disease- ... ...

    Abstract The ongoing epidemic of mpox, namely human monkeypox virus (MPXV) infection, requires rapid and reliable laboratory diagnosis. We report on the QIAstat-Dx viral vesicular panel PCR assay that allows the detection of (within 75 min) six vesicular disease-causing viruses, including MPXV. We analyzed 168 clinical samples, known to be positive (51 samples) or negative (117 samples) for MPXV clade II, obtained from patients at their mpox diagnosis or follow-up. QIAstat assay results were compared to those of a MPXV-specific reference PCR assay. The QIAstat assay detected MPXV (clade II) in 51 (100%) of 51 samples and did not detect MPXV in 117 (100%) of 117 samples, resulting in a positive or negative agreement of 100% (95% CI, 93.0-100) and 100% (95% CI, 96.8-100), respectively. Of the 20 patients diagnosed with mpox, 18 (90.0%) had at least a vesicular swab and 1 (5.0%) had only an oropharyngeal swab positive for MPXV. At mpox follow-ups, 2 (10.0%) of 20 patients had first-time positive whole blood samples. Thirteen MPXV-negative samples were positive for mpox-mimicking viruses. Our findings show the excellent performance of the QIAstat-Dx assay for MPXV detection in clinical samples. Further studies are needed before considering a large-scale application of the QIAstat-Dx assay.
    Language English
    Publishing date 2023-10-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11102513
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  4. Article: Susceptibility of Meropenem-Resistant and/or Carbapenemase-Producing Clinical Isolates of

    Cortazzo, Venere / Posteraro, Brunella / Menchinelli, Giulia / Liotti, Flora Marzia / D'Inzeo, Tiziana / Fiori, Barbara / Luzzaro, Francesco / Sanguinetti, Maurizio / Spanu, Teresa

    Antibiotics (Basel, Switzerland)

    2022  Volume 11, Issue 8

    Abstract: This study aimed to assess the comparability of in vitro susceptibility testing methods to ceftazidime-avibactam (CZA) and ceftolozane-tazobactam (C/T). Meropenem-resistant and/or carbapenemase-producing clinical isolates ... ...

    Abstract This study aimed to assess the comparability of in vitro susceptibility testing methods to ceftazidime-avibactam (CZA) and ceftolozane-tazobactam (C/T). Meropenem-resistant and/or carbapenemase-producing clinical isolates of
    Language English
    Publishing date 2022-07-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics11081023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Assessment of SARS-CoV-2 RNA Test Results Among Patients Who Recovered From COVID-19 With Prior Negative Results.

    Liotti, Flora Marzia / Menchinelli, Giulia / Marchetti, Simona / Posteraro, Brunella / Landi, Francesco / Sanguinetti, Maurizio / Cattani, Paola

    JAMA internal medicine

    2021  Volume 181, Issue 5, Page(s) 702–704

    MeSH term(s) COVID-19 ; Humans ; Negative Results ; RNA, Viral/genetics ; Recurrence ; SARS-CoV-2
    Chemical Substances RNA, Viral
    Keywords covid19
    Language English
    Publishing date 2021-01-21
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 2699338-7
    ISSN 2168-6114 ; 2168-6106
    ISSN (online) 2168-6114
    ISSN 2168-6106
    DOI 10.1001/jamainternmed.2020.7570
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.119: Show issues Location:
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  6. Article: SARS-CoV-2 Antigen Test Results to Infer Active or Non-Active Virus Replication Status in COVID-19 Patients.

    De Angelis, Giulia / Menchinelli, Giulia / Liotti, Flora Marzia / Marchetti, Simona / Salustri, Alessandro / Vella, Antonietta / Santangelo, Rosaria / Posteraro, Brunella / Sanguinetti, Maurizio

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 6

    Abstract: We used nasopharyngeal swab samples of patients with a symptomatic (n = 82) or asymptomatic (n = 20) coronavirus disease 2019 (COVID-19) diagnosis to assess the ability of antigen detection tests to infer active (potentially transmissible) or inactive ( ... ...

    Abstract We used nasopharyngeal swab samples of patients with a symptomatic (n = 82) or asymptomatic (n = 20) coronavirus disease 2019 (COVID-19) diagnosis to assess the ability of antigen detection tests to infer active (potentially transmissible) or inactive (potentially non-transmissible) infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Using the subgenomic RNA (sgRNA) as an active replication marker of SARS-CoV-2, 48 (76.2%), 56 (88.9%), and 63 (100%) of 63 samples with sgRNA positive results tested positive with the SD BIOSENSOR STANDARD Q COVID-19 Ag (Standard Q), the SD BIOSENSOR STANDARD F COVID-19 Ag FIA (Standard F), or the Fujirebio LUMIPULSE G SARS-CoV-2 Ag (Lumipulse) assay, respectively. Conversely, 37 (94.9%), 29 (74.4%), and 7 (17.9%) of 39 samples with sgRNA negative results tested negative with Standard Q, Standard F, or Lumipulse, respectively. Stratifying results by the number of days of symptoms before testing revealed that most antigen positive/sgRNA positive results were among samples tested at 2-7 days regardless of the assay used. Conversely, most antigen negative/sgRNA negative results were among samples tested at 16-30 days only when Standard Q or Standard F were used. In conclusion, based on our findings, a negative antigen test, especially with the Lumipulse assay, or a positive antigen test, especially with the Standard F assay, may suggest, respectively, the absence or presence of replication-competent SARS-CoV-2.
    Language English
    Publishing date 2022-05-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12061338
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: SARS-CoV-2 viral load and replication in postmortem examinations.

    Grassi, Simone / Arena, Vincenzo / Cattani, Paola / Dell'Aquila, Marco / Liotti, Flora Marzia / Sanguinetti, Maurizio / Oliva, Antonio

    International journal of legal medicine

    2022  Volume 136, Issue 3, Page(s) 935–939

    Abstract: We examined 29 autopsy cases (investigated between October 2020 and February 2021) whose postmortem swabs tested positive for SARS-CoV-2. Twenty-two of 29 cases died while hospitalized (H), while the remaining 7 cases were not hospitalized (NH). Since we ...

    Abstract We examined 29 autopsy cases (investigated between October 2020 and February 2021) whose postmortem swabs tested positive for SARS-CoV-2. Twenty-two of 29 cases died while hospitalized (H), while the remaining 7 cases were not hospitalized (NH). Since we included only cases in which the time since death was known (excluding unwitnessed NH deaths), the interval between death and postmortem swab(s) was registered, with a mean NH value of 5.50 days and a mean H value of 3.98 days. The mean age of NH was 65 years, while H were older (mean age: 73 years). Twenty-eight nasopharyngeal and 27 lungs postmortem swabs were obtained and real-time reverse transcriptase‒polymerase chain reaction assay for total and replicative SARS-CoV-2 RNA and mRNA detection was performed. Although the mean death-postmortem swabs interval was higher in NH than in H, the mean viral load of NH was higher than that of H (2.53 × 10
    MeSH term(s) Aged ; Autopsy ; COVID-19/diagnosis ; Humans ; RNA, Messenger ; RNA, Viral ; SARS-CoV-2 ; Viral Load
    Chemical Substances RNA, Messenger ; RNA, Viral
    Language English
    Publishing date 2022-01-24
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-021-02753-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Un I Zs.312: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  8. Article ; Online: SARS-CoV-2 Antigen Test Results to Infer Active or Non-Active Virus Replication Status in COVID-19 Patients

    Giulia De Angelis / Giulia Menchinelli / Flora Marzia Liotti / Simona Marchetti / Alessandro Salustri / Antonietta Vella / Rosaria Santangelo / Brunella Posteraro / Maurizio Sanguinetti

    Diagnostics, Vol 12, Iss 1338, p

    2022  Volume 1338

    Abstract: We used nasopharyngeal swab samples of patients with a symptomatic (n = 82) or asymptomatic (n = 20) coronavirus disease 2019 (COVID-19) diagnosis to assess the ability of antigen detection tests to infer active (potentially transmissible) or inactive ( ... ...

    Abstract We used nasopharyngeal swab samples of patients with a symptomatic (n = 82) or asymptomatic (n = 20) coronavirus disease 2019 (COVID-19) diagnosis to assess the ability of antigen detection tests to infer active (potentially transmissible) or inactive (potentially non-transmissible) infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Using the subgenomic RNA (sgRNA) as an active replication marker of SARS-CoV-2, 48 (76.2%), 56 (88.9%), and 63 (100%) of 63 samples with sgRNA positive results tested positive with the SD BIOSENSOR STANDARD Q COVID-19 Ag (Standard Q), the SD BIOSENSOR STANDARD F COVID-19 Ag FIA (Standard F), or the Fujirebio LUMIPULSE G SARS-CoV-2 Ag (Lumipulse) assay, respectively. Conversely, 37 (94.9%), 29 (74.4%), and 7 (17.9%) of 39 samples with sgRNA negative results tested negative with Standard Q, Standard F, or Lumipulse, respectively. Stratifying results by the number of days of symptoms before testing revealed that most antigen positive/sgRNA positive results were among samples tested at 2–7 days regardless of the assay used. Conversely, most antigen negative/sgRNA negative results were among samples tested at 16–30 days only when Standard Q or Standard F were used. In conclusion, based on our findings, a negative antigen test, especially with the Lumipulse assay, or a positive antigen test, especially with the Standard F assay, may suggest, respectively, the absence or presence of replication-competent SARS-CoV-2.
    Keywords antigen detection ; COVID-19 ; reverse transcription-PCR ; SARS-CoV-2 ; subgenomic RNA ; virus replication ; Medicine (General) ; R5-920
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Bordetella pertussis DNA detected in a tracheostomized child blood before seroconversion.

    Liotti, Flora Marzia / De Angelis, Giulia / Speziale, Domenico / Morandotti, Grazia Angela / Genovese, Orazio / Sanguinetti, Maurizio / Posteraro, Brunella

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2020  Volume 40, Issue 1, Page(s) 205–208

    MeSH term(s) Bordetella pertussis/genetics ; Bordetella pertussis/isolation & purification ; Child, Preschool ; DNA, Bacterial/blood ; Diagnosis, Differential ; Fatal Outcome ; Humans ; Male ; Seroconversion ; Spasms, Infantile ; Thrombophilia ; Tracheotomy ; Whooping Cough/blood ; Whooping Cough/diagnosis
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2020-07-13
    Publishing country Germany
    Document type Case Reports ; Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-020-03988-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1732: Show issues Location:
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  10. Article ; Online: A New PCR-Based Assay for Testing Bronchoalveolar Lavage Fluid Samples from Patients with Suspected

    Liotti, Flora Marzia / Posteraro, Brunella / De Angelis, Giulia / Torelli, Riccardo / De Carolis, Elena / Speziale, Domenico / Menchinelli, Giulia / Spanu, Teresa / Sanguinetti, Maurizio

    Journal of fungi (Basel, Switzerland)

    2021  Volume 7, Issue 9

    Abstract: To support the clinical laboratory diagnosis ... ...

    Abstract To support the clinical laboratory diagnosis of
    Language English
    Publishing date 2021-08-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof7090681
    Database MEDical Literature Analysis and Retrieval System OnLINE

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