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Article ; Online: Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model.

Dou, Lu / Gong, Xue / Wu, Qing / Mou, Fangzheng

2021 Volume 59, Issue 1, Page(s) 789–798

Abstract: Context: Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy ...

Abstract	Context: Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. Objective: To study the effect and possible mechanism of SXF to restore haematopoietic function. Materials and methods: Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10-12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Results: Red blood cell relative changes in the SXF group (low: -5.50 ± 1.58%; medium: -11.11 ± 4.15%; high: -8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all Conclusions: SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.
MeSH term(s)	Anemia/blood ; Anemia/chemically induced ; Anemia/drug therapy ; Animals ; Cyclophosphamide/toxicity ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/therapeutic use ; Erythrocyte Count ; Female ; Male ; Mice ; Mice, Inbred BALB C ; Treatment Outcome
Chemical Substances	Drugs, Chinese Herbal ; Cyclophosphamide (8N3DW7272P)
Language	English
Publishing date	2021-07-12
Publishing country	England
Document type	Journal Article
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2021.1941133
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Article ; Online: Traditional Chinese Medicine formula Bu-Shen-Huo-Xue-Fang (BSHXF) protects nucleus pulposus cells against the inflammatory and oxidative stress-induced degenerative changes.

Li, Linghui / Wei, Xu / Li, Kaiming / Gong, Hao / Zhu, Liguo / Yang, Shaofeng / Wang, Shangquan / Gu, Jinyu / Chen, Ming / Yin, Xunlu / Zhan, Jiawen / Feng, Minshan / Yu, Jie / Sun, Wu / Chen, Xin

Journal of pharmaceutical and biomedical analysis

2023 Volume 236, Page(s) 115656

Abstract: ... NPCs) and extracellular matrix (ECM) (IDD). Bu-Shen-Huo-Xue-Fang (BSHXF ...

Abstract	Degeneration of the intervertebral disc is primarily caused by the loss of nucleus pulposus cells (NPCs) and extracellular matrix (ECM) (IDD). Bu-Shen-Huo-Xue-Fang (BSHXF), a traditional Chinese medicine decoction, has been used to treat IDD in clinical; nevertheless, the active components and underlying molecular mechanisms remain unknown. BSHXF improved IL-1β and H
Language	English
Publishing date	2023-08-16
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2023.115656
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Article ; Online: Disentangling the Pelodiscus axenaria complex, with the description of a new Chinese species and neotype designation for P. axenaria (Zhou, Zhang amp; Fang, 1991).

Gong, Shiping / Fritz, Uwe / Vamberger, Melita / Gao, Yangchun / Farkas, Balzs

Zootaxa

2022 Volume 5125, Issue 2, Page(s) 131–143

Abstract: ... China. Also, the application of the name P. axenaria (Zhou, Zhang Fang, 1991) is clarified ...

Abstract	We describe a new species from the Pelodiscus axenaria complex from Hunan and Jiangxi Provinces, China. Also, the application of the name P. axenaria (Zhou, Zhang Fang, 1991) is clarified by designating a neotype for this species. Besides its genetic divergence, the new species differs from all other Pelodiscus species, including the two other taxa constituting the complex (P. axenaria, P. huangshanensis), in the following combination of morphological traits: (1) small adult size, <15 cm carapace length; (2) carapace distinctly keeled, more or less strongly tuberculated, usually olive clay-coloured and adorned with greenish black marbling; (3) plastron yellowish white, typically immaculate except for a blurred-edged blotch behind each axilla that does not extend to the entoplastron and some slight black suffusion along its anterior border; (4) underside of the leathery margin of the carapace with varying amounts of dark pigmentation; (5) head olive clay-coloured with numerous black splotches; (6) chin grey brown with pale stipples, throat dark grey, finely spotted with black; (7) neck with a wide yellow lateral band stretching from the tympanum posteriorly, which tends to fade with age; (8) entoplastron boomerang-shaped, the amount of bending of the transverse bar between the two posteriolaterally directed rami >90.
MeSH term(s)	Animals ; Clay ; Reptiles
Chemical Substances	Clay (T1FAD4SS2M)
Language	English
Publishing date	2022-04-08
Publishing country	New Zealand
Document type	Journal Article
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.5125.2.2
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Article ; Online: Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Dou, Lu / Gong, Xue / Wu, Qing / Mou, Fangzheng

Pharmaceutical Biology. 2021 Jan. 1, v. 59, no. 1 p.787-796

2021

Abstract: Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. To study ...

Abstract	Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. To study the effect and possible mechanism of SXF to restore haematopoietic function. Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10–12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Red blood cell relative changes in the SXF group (low: −5.50 ± 1.58%; medium: −11.11 ± 4.15%; high: −8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all p < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice. Splenic index in the medium group (4.44 ± 0.46%) relative negative control (3.38 ± 0.10%) significantly improved (p < 0.01) in female mice. Using network pharmacology, 77 active components and 337 targets were screened from SXF. These targets are closely related to the mitogen-activated protein kinase pathway. SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.
Keywords	Oriental traditional medicine ; anemia ; cyclophosphamide ; erythrocytes ; females ; femur ; genes ; hemoglobin ; histopathology ; males ; mice ; mitogen-activated protein kinase ; models ; pharmacology ; protein-protein interactions ; spleen ; therapeutics ; Network pharmacology ; red blood cell ; haematopoiesis
Language	English
Dates of publication	2021-0101
Size	p. 787-796.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	1440131-9
ISSN	1744-5116 ; 1388-0209
ISSN (online)	1744-5116
ISSN	1388-0209
DOI	10.1080/13880209.2021.1941133
Database	NAL-Catalogue (AGRICOLA)

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Article: Chuan Huang Fang combining reduced glutathione in treating acute kidney injury (grades 1-2) on chronic kidney disease (stages 2-4): A multicenter randomized controlled clinical trial.

Chen, Ling / Ye, Zi / Wang, Danjun / Liu, Jianlian / Wang, Qian / Wang, Chen / Xu, Bing / Gong, Xuezhong

Frontiers in pharmacology

2022 Volume 13, Page(s) 969107

Abstract: ... Our previously single-center clinical studies indicated Chuan Huang Fang (CHF) might have nephroprotection in AKI ...

Abstract	Lack of effective drugs for acute kidney injury (AKI) grades 1-2 is a crucial challenge in clinic. Our previously single-center clinical studies indicated Chuan Huang Fang (CHF) might have nephroprotection in AKI on chronic kidney disease (CKD) (A on C) patients by preventing oxidant damage and inhibiting inflammation. Reduced glutathione (RG) has recently been shown to increase the clinical effectiveness of high-flux hemodialysis among patients with severe AKI. In this multicenter randomized controlled clinical study, we designed a new protocol to assess the efficacy and safety of CHF combining RG in patients with A on C. We also explored therapeutic mechanisms from renal fibrosis biomarkers. 98 participants were randomly and equally divided into the RG and RG + CHF subgroups. The RG and RG + CHF groups received general treatments with RG and a combination of RG and CHF, respectively. The therapy lasted for 2 weeks. In this study, the primary assessment result was a difference in the slope of serum creatinine (Scr) over the course of 2 weeks. The secondary evaluation outcomes were alterations in blood urea nitrogen (BUN), uric acid (UA), estimated glomerular filtration rate (eGFR), urinary AKI biomarkers, renal fibrosis biomarkers (transforming growth factor-
Language	English
Publishing date	2022-10-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.969107
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Article ; Online: Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer.

Ke, Bin / Wu, Xinlin / Yang, Qiong / Huang, Yuanyuan / Wang, Fang / Gong, Yuxin / Liu, Junfang / Shi, Lin

Bioscience reports

2019 Volume 39, Issue 6

Abstract: ... In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and ...

Abstract	Traditional Chinese Medicine (TCM) has been recognized to be conducive to enhancing the efficiency and reducing the side effects in the whole course of cancer treatment. The mechanisms of TCM/chemotherapy combination involved with interleukin-7 (IL-7) potentially enhance immune responses against tumor. In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and investigated its roles in chemotherapy in non-small cell lung cancer (NSCLC). The mice bared with tumor were treated with cisplatin (DDP) and simultaneously administrated with/without low, medium and high doses of TCMs (effective content: 0.5, 2.0 and 8.0 g/per mice) via oral gavage. The results indicated that combination of TCM further elevated the therapy efficiency of DDP in a dose-dependent manner. The growth of tumor cells was estimated by Ki-67 stain and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay. The addition of TCM to the DDP treatment could significantly decrease the expression of Ki-67 and promote the apoptosis of tumor cells. In addition, the serum IL-7 level was down-regulated by DDP but restored by the treatment of TCM. The expression of IL-7 and its receptor IL-7R in tumor tissues was also recovered by TCM. Furthermore, the side effect from bone marrow suppression (myelosuppression) induced by DDP were assessed. TCM could abrogate DDP-induced apoptosis of bone marrow and also remarkably induced the expressions of IL-7 and hematopoietic growth factors including G-CSF, GM-CSF, SCF, and SDF-1 in bone marrow. These data indicated that this TCM combined with DDP showed superior anti-tumor effects with reduced myelosuppression via up-regulating IL-7.
MeSH term(s)	A549 Cells ; Animals ; Apoptosis/drug effects ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/metabolism ; Carcinoma, Non-Small-Cell Lung/pathology ; Cisplatin/pharmacology ; Drugs, Chinese Herbal/pharmacology ; Humans ; Interleukin-7/metabolism ; Lung Neoplasms/drug therapy ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Male ; Mice ; Mice, Nude ; Neoplasm Proteins/metabolism ; Xenograft Model Antitumor Assays
Chemical Substances	Drugs, Chinese Herbal ; IL7 protein, human ; Interleukin-7 ; Neoplasm Proteins ; Cisplatin (Q20Q21Q62J)
Language	English
Publishing date	2019-06-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	764946-0
ISSN	1573-4935 ; 0144-8463
ISSN (online)	1573-4935
ISSN	0144-8463
DOI	10.1042/BSR20190052
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Article: Efficacy and Safety of Chuan Huang Fang Combining Reduced Glutathione in Treating Acute Kidney Injury (Grades 1-2) on Chronic Kidney Disease (Stages 2-4): Study Protocol for a Multicenter Randomized Controlled Clinical Trial.

Chen, Ling / Gong, Xuezhong

Evidence-based complementary and alternative medicine : eCAM

2022 Volume 2022, Page(s) 1099642

Abstract: ... in clinic. Chuan Huang Fang (CHF) is a Chinese herbal formulation developed for the treatment of A on C ...

Abstract	Background: Acute kidney injury (AKI) is a global public health challenge resulting in considerable morbidity and mortality. AKI on chronic kidney disease (CKD) (AKI on CKD, A on C) accounts for about a third of total AKI. For severe AKI grade 3, renal replacement therapy (RRT) should be implemented in time. However, the lack of recognized drug treatment method for AKI grades 1-2 is a crucial problem in clinic. Chuan Huang Fang (CHF) is a Chinese herbal formulation developed for the treatment of A on C from the Shanghai Municipal Hospital of Traditional Chinese Medicine. Our previous studies suggested that CHF might effectively protect renal functions of A on C patients. As a widely used antioxidant in clinic, reduced glutathione (RG) is reported to improve the clinical efficacy of high-flux hemodialysis (HFHD) in severe AKI patients recently. To address the crucial problem mentioned above, thus we design a new clinical protocol of CHF combining RG and try to evaluate the efficacy and safety of this protocol in treating patients diagnosed with CKD stages 2-4 complicated with AKI grades 1-2. Methods: This is a multicenter randomized controlled clinical trial. We intend to enroll 162 participants, and these participants will be divided into the RG group, the CHF group, and the RG + CHF group randomly assigning 1 : 1 : 1 principle. The RG group will be general treatments combining RG, the CHF group will be general treatments combining CHF, and the RG + CHF group will be general treatments combining RG and CHF. The duration of treatment will last two weeks. The primary evaluation outcome will be the change in the slope of serum creatinine (Scr) over 2 weeks. Secondary evaluation outcomes include changes in blood urea nitrogen (BUN), estimated glomerular filtration rate (eGFR), urinary AKI biomarkers (neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), gamma-glutamyl transpeptidase (
Language	English
Publishing date	2022-03-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2022/1099642
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Article ; Online: Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis.

Gong, Shan-Shan / Fan, Yi-Hong / Wang, Shi-Yi / Han, Qing-Qing / Lv, Bin / Xu, Yi / Chen, Xi / He, Yao-Er

World journal of gastroenterology

2018 Volume 24, Issue 16, Page(s) 1766–1778

Abstract: ... healing of dextran sulfate sodium (DSS)-induced colitis and the effect of Tong-Xie-Yao-Fang (TXYF) on CRH ...

Abstract	Aim: To explore the significance of corticotropin-releasing hormone (CRH)-receptor (R)2 in mucosal healing of dextran sulfate sodium (DSS)-induced colitis and the effect of Tong-Xie-Yao-Fang (TXYF) on CRH-R2 expression and regulation. Methods: Ulcerative colitis was induced in mice by administration of 3% (w/v) DSS for 7 d. Once the model was established, mice were administered urocortin-2 (30 μg/kg), a peptide which binds exclusively to CRH-R2, or various doses of aqueous TXYF extracts (2.8-11.2 g/kg), a CRH-R2 antagonist Astressin (Ast)2B (20 μg/kg), Ast2B + Ucn2, or Ast2B with various doses of aqueous TXYF extracts for 9 d. Colonic mucosal permeability was then evaluated by measuring the fluorescence intensity in serum. The colitis disease activity index (DAI), histology, body weight loss and colon length were assessed to evaluate the condition of colitis. Terminal deoxynucleotidyl transferase dUTP nick-end labeling was used to detect apoptosis of the intestinal epithelial cells. The expression level of Ki-67 represented the proliferation of colonic epithelial cells and was detected by immunohistochemistry. The expression levels of inflammation cytokines IL-6, TNF-α and CXCL-1 were examined in colon tissues using real-time PCR and ELISA kits. Results: Compared with the DSS group, mice treated with the CRH-R2 antagonist Ast2B showed greater loss of body weight, shorter colon lengths (4.90 ± 0.32 Conclusion: CRH-R2 activates the intestinal mucosal antiinflammatory response by regulating migration, proliferation and apoptosis of intestinal epithelial cells in colitis-induced mice, and plays an important antiinflammatory role. TXYF promotes mucosal repair in colitis mice by regulating CRH-R2.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Apoptosis/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Chemokine CXCL1/metabolism ; Colitis/chemically induced ; Colitis/drug therapy ; Colitis/metabolism ; Colitis/pathology ; Colon/drug effects ; Colon/metabolism ; Colon/pathology ; Dextran Sulfate ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Interleukin-6/metabolism ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/pathology ; Ki-67 Antigen/metabolism ; Male ; Mice, Inbred ICR ; Permeability ; Receptors, Corticotropin-Releasing Hormone/metabolism ; Signal Transduction/drug effects ; Tumor Necrosis Factor-alpha/metabolism ; Wound Healing/drug effects
Chemical Substances	Anti-Inflammatory Agents ; CRF receptor type 2 ; Chemokine CXCL1 ; Cxcl1 protein, mouse ; Drugs, Chinese Herbal ; Interleukin-6 ; Ki-67 Antigen ; Receptors, Corticotropin-Releasing Hormone ; Tumor Necrosis Factor-alpha ; interleukin-6, mouse ; tongxie-yaofang ; Dextran Sulfate (9042-14-2)
Language	English
Publishing date	2018-04-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v24.i16.1766
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Article ; Online: Efficacy and Safety of Chuan Huang Fang Combining Reduced Glutathione in Treating Acute Kidney Injury (Grades 1–2) on Chronic Kidney Disease (Stages 2–4)

Ling Chen / Xuezhong Gong

Evidence-Based Complementary and Alternative Medicine, Vol

Study Protocol for a Multicenter Randomized Controlled Clinical Trial

2022 Volume 2022

Abstract: ... Fang (CHF) is a Chinese herbal formulation developed for the treatment of A on C from the Shanghai ...

Abstract	Background. Acute kidney injury (AKI) is a global public health challenge resulting in considerable morbidity and mortality. AKI on chronic kidney disease (CKD) (AKI on CKD, A on C) accounts for about a third of total AKI. For severe AKI grade 3, renal replacement therapy (RRT) should be implemented in time. However, the lack of recognized drug treatment method for AKI grades 1–2 is a crucial problem in clinic. Chuan Huang Fang (CHF) is a Chinese herbal formulation developed for the treatment of A on C from the Shanghai Municipal Hospital of Traditional Chinese Medicine. Our previous studies suggested that CHF might effectively protect renal functions of A on C patients. As a widely used antioxidant in clinic, reduced glutathione (RG) is reported to improve the clinical efficacy of high-flux hemodialysis (HFHD) in severe AKI patients recently. To address the crucial problem mentioned above, thus we design a new clinical protocol of CHF combining RG and try to evaluate the efficacy and safety of this protocol in treating patients diagnosed with CKD stages 2–4 complicated with AKI grades 1–2. Methods. This is a multicenter randomized controlled clinical trial. We intend to enroll 162 participants, and these participants will be divided into the RG group, the CHF group, and the RG + CHF group randomly assigning 1 : 1 : 1 principle. The RG group will be general treatments combining RG, the CHF group will be general treatments combining CHF, and the RG + CHF group will be general treatments combining RG and CHF. The duration of treatment will last two weeks. The primary evaluation outcome will be the change in the slope of serum creatinine (Scr) over 2 weeks. Secondary evaluation outcomes include changes in blood urea nitrogen (BUN), estimated glomerular filtration rate (eGFR), urinary AKI biomarkers (neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), gamma-glutamyl transpeptidase (γ-GT), etc.), traditional Chinese medicine (TCM) symptoms, inflammatory indicators, and oxidative stress ...
Keywords	Other systems of medicine ; RZ201-999
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book: Zhong yao cai pao zhi jia gong fang fa tu jie

Gong, Qianfeng

2010

Author's details	Gong Qianfeng, Yuan Xiaoping, Zhong Lingyun zhu bian
MeSH term(s)	Materia Medica ; Formularies as Topic ; Medicine, Chinese Traditional/methods
Language	Chinese
Size	8, 151 p. :, ill.
Edition	Di 1 ban.
Publisher	Ren min wei sheng chu ban she
Publishing place	Beijing
Document type	Book
ISBN	9787117136662 ; 7117136669
Database	Catalogue of the US National Library of Medicine (NLM)

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