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Article ; Online: Facilitating tissue infiltration and angiogenesis in a tubular collagen scaffold.

Gérard, Catherine / Doillon, Charles J

Journal of biomedical materials research. Part A

2010 Volume 93, Issue 2, Page(s) 615–624

Abstract: Among different strategies to provide blood supply to tissue-engineered devices and implants, the use of arteriovenous loops and bundles has been proposed. The aim of this study was to compare the vascularization and healing processes that took place in ... ...

Abstract	Among different strategies to provide blood supply to tissue-engineered devices and implants, the use of arteriovenous loops and bundles has been proposed. The aim of this study was to compare the vascularization and healing processes that took place in a one-end closed tubular collagen-based scaffold at different implantation sites in mice. These conditions were in the presence or absence of heparin and/or bone marrow cells. By 30 days, very few cell infiltrations were observed in the dorsal subcutaneous and peritoneal implants at any conditions; however, the presence of heparin and bone marrow cells improved cell infiltration toward an inflammatory reaction. The insertion of an arteriovenous bundle into the central cavity of the scaffold resulted in partial wound tissue infiltration in the control scaffolds implanted subcutaneously in the hind limb. In similar conditions, the presence of bone marrow cells and heparin resulted in dense wound tissue with numerous capillaries and a significant amount of newly deposited collagen fibers. The design of a central cavity in a porous scaffold with one closed end may facilitate invasion from the central part of the implant toward the implant wall. In addition, the presence of both a vascular component and stem/progenitor cells may lead to a vascularized implant while limiting the inflammatory reaction.
MeSH term(s)	Animals ; Cattle ; Collagen/chemistry ; Collagen/metabolism ; Humans ; Implants, Experimental ; Inflammation/metabolism ; Inflammation/pathology ; Mice ; Microvessels/cytology ; Microvessels/metabolism ; Neovascularization, Physiologic/physiology ; Tissue Culture Techniques/instrumentation ; Tissue Culture Techniques/methods ; Tissue Engineering/instrumentation ; Tissue Engineering/methods ; Tissue Scaffolds/chemistry
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	2010-05
Publishing country	United States
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2099989-6
ISSN	1552-4965 ; 1549-3296 ; 0021-9304
ISSN (online)	1552-4965
ISSN	1549-3296 ; 0021-9304
DOI	10.1002/jbm.a.32568
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Zs.A 6195: Show issues

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Article ; Online: Inhibition of 17beta-hydroxysteroid dehydrogenase type 7 modulates breast cancer protein profile and enhances apoptosis by down-regulating GRP78.

Wang, Xiao-Qiang / Aka, Juliette A / Li, Tang / Xu, Dan / Doillon, Charles J / Lin, Sheng-Xiang

The Journal of steroid biochemistry and molecular biology

2017 Volume 172, Page(s) 188–197

Abstract: 17beta-hydroxysteroid dehydrogenase type 7 (17β-HSD7) promotes breast cancer cell growth via dual-catalytic activity by modulating estradiol and DHT. Here, we clarified the expression pattern of 17β-HSD7 in postmenopausal luminal A type breast cancer ... ...

Abstract	17beta-hydroxysteroid dehydrogenase type 7 (17β-HSD7) promotes breast cancer cell growth via dual-catalytic activity by modulating estradiol and DHT. Here, we clarified the expression pattern of 17β-HSD7 in postmenopausal luminal A type breast cancer with The Cancer Genome Atlas (TCGA) cohort. The impact of 17β-HSD7 inhibition on the proteome of MCF-7 cells was investigated and on cell apoptosis was revealed. MCF-7 cells were treated with an efficient inhibitor of 17β-HSD7 (INH7) or with vehicle, and a differential proteomics study was performed using two-dimensional (2D) gel electrophoresis followed by mass spectrometry and ingenuity pathway analysis (IPA). Cell apoptosis was analyzed by flow cytometry, followed by reverse transcription quantitative real-time PCR (RT-qPCR) and Western blot to investigate the expression of apoptosis-related genes. Our data showed 17β-HSD7 is amplified in primary and progressive breast cancer, inhibition of 17β-HSD7 in MCF-7 cells modulated 104 proteins primarily involved in cell death/survival, cell growth and DNA processing. The expression of 78kDa glucose-regulated protein (GRP78) and anti-apoptosis factor Bcl-2 were significantly suppressed via 17β-HSD7 inhibition with INH7, consequently induced MCF-7 cell apoptosis. However, INH7 treatment of T47D, another widely used epithelial ER+ breast cancer cell line, led to an up-regulation of GRP78 expression, resulting in a limited increase in apoptosis. These results suggest cell-specific effects of INH7 in the breast cancer, which is interesting for further study. An combinatory effect on apoptosis by INH7 and Letrozole (aromatase inhibitor) was further demonstrated in MCF-7. Down-regulation of GRP78 via 17β-HSD7 inhibition enhances cell apoptosis in response to Letrozole. This study highlights GRP78 as a key regulator related to 17β-HSD7 inhibition and effect. Taken together, results from the present study suggest a hypothesis that inhibition of 17β-HSD7 would be a complementary strategy to Letrozole by suppression of GRP78 in ER+ breast cancer. However, from a research perspective, further studies have to be carried out with more breast cancer cell lines as well as in vivo model to assess the efficacy of inhibitor combination.
MeSH term(s)	17-Hydroxysteroid Dehydrogenases/antagonists & inhibitors ; 17-Hydroxysteroid Dehydrogenases/genetics ; 17-Hydroxysteroid Dehydrogenases/metabolism ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Apoptosis/genetics ; Aromatase Inhibitors/pharmacology ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Drug Synergism ; Enzyme Inhibitors/pharmacology ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Letrozole ; Molecular Sequence Annotation ; Nitriles/pharmacology ; Organ Specificity ; Postmenopause ; Protein Interaction Mapping ; Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors ; Proto-Oncogene Proteins c-bcl-2/genetics ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Receptors, G-Protein-Coupled/antagonists & inhibitors ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Signal Transduction ; Triazoles/pharmacology
Chemical Substances	Antineoplastic Agents ; Aromatase Inhibitors ; BCL2 protein, human ; Enzyme Inhibitors ; Nitriles ; Proto-Oncogene Proteins c-bcl-2 ; RNA, Small Interfering ; Receptors, G-Protein-Coupled ; Triazoles ; Letrozole (7LKK855W8I) ; 17-Hydroxysteroid Dehydrogenases (EC 1.1.-) ; 3 (or 17)-beta-hydroxysteroid dehydrogenase (EC 1.1.1.51) ; molecular chaperone GRP78 (YCYIS6GADR)
Language	English
Publishing date	2017-06-20
Publishing country	England
Document type	Journal Article
ZDB-ID	1049188-0
ISSN	1879-1220 ; 0960-0760
ISSN (online)	1879-1220
ISSN	0960-0760
DOI	10.1016/j.jsbmb.2017.06.009
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Zs.A 708: Show issues

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Article ; Online: Histological study of stem-like cells in human colon adenocarcinoma at different stages of the disease.

Florianova, L / Orain, M / Têtu, B / Doillon, C J

Biotechnic & histochemistry : official publication of the Biological Stain Commission

2013 Volume 88, Issue 5, Page(s) 222–234

Abstract: The presence of stem-like cells in tumors reflects the invasive character of the disease; however, their identification is controversial. We investigated the distribution of CD133, CD44 and CD24 using histological sections and tissue microarrays (TMAs) ... ...

Abstract	The presence of stem-like cells in tumors reflects the invasive character of the disease; however, their identification is controversial. We investigated the distribution of CD133, CD44 and CD24 using histological sections and tissue microarrays (TMAs) of human colon adenocarcinoma obtained from patients with and without lymph node metastases and/or liver metastases. Immunohistochemical staining was combined with nuclear staining and evaluated quantitatively using image analysis software. Sections of normal colon mucosa, the primary tumor, lymph node, and liver also were analyzed qualitatively and compared to the quantitative method, which was more accurate. In most tissues, the expression of CD44 and CD24 was relatively low compared to CD133, with some variations. CD133 also was expressed in the normal colon mucosa and to a lesser degree in normal hepatic parenchyma. Liver metastases exhibited significantly greater CD133 staining compared to normal colon mucosa, primary tumor and lymph node metastases. Moreover, lymph node metastases obtained from patients with liver metastases expressed significantly greater CD133 staining than those obtained from patients without liver metastasis. Our data suggest that CD133 expression in lymph node metastases may be of value for prognosis of the disease.
MeSH term(s)	Adenocarcinoma/pathology ; Aged ; Aged, 80 and over ; Antigens, CD/genetics ; Antigens, CD/metabolism ; Colonic Neoplasms/pathology ; Female ; Gene Expression Regulation, Neoplastic/physiology ; Humans ; Male ; Middle Aged ; Neoplastic Stem Cells/pathology
Chemical Substances	Antigens, CD
Language	English
Publishing date	2013-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1069349-x
ISSN	1473-7760 ; 1052-0295
ISSN (online)	1473-7760
ISSN	1052-0295
DOI	10.3109/10520295.2012.758310
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Article ; Online: Synergistic control of sex hormones by 17β-HSD type 7: a novel target for estrogen-dependent breast cancer.

Wang, Xiaoqiang / Gérard, Catherine / Thériault, Jean-François / Poirier, Donald / Doillon, Charles J / Lin, Sheng-Xiang

Journal of molecular cell biology

2015 Volume 7, Issue 6, Page(s) 568–579

Abstract: 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 is known as a critical target to block the final step of estrogen production in estrogen-dependent breast cancer. Recent confirmation of the role of dyhydroxytestosterone (DHT) in counteracting estrogen- ... ...

Abstract	17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 is known as a critical target to block the final step of estrogen production in estrogen-dependent breast cancer. Recent confirmation of the role of dyhydroxytestosterone (DHT) in counteracting estrogen-induced cell growth prompted us to study the reductive 17β-HSD type 7 (17β-HSD7), which activates estrone while markedly inactivating DHT. The role of DHT in breast cancer cell proliferation is demonstrated by its independent suppression of cell growth in the presence of a physiological concentration of estradiol (E2). Moreover, an integral analysis of a large number of clinical samples in Oncomine datasets demonstrated the overexpression of 17β-HSD7 in breast carcinoma. Inhibition of 17β-HSD7 in breast cancer cells resulted in a lower level of E2 and a higher level of DHT, successively induced regulation of cyclinD1, p21, Bcl-2, and Bik, consequently arrested cell cycle in the G(0)/G(1) phase, and triggered apoptosis and auto-downregulation feedback of the enzyme. Such inhibition led to significant shrinkage of xenograft tumors with decreased cancer cell density and reduced 17β-HSD7 expression. Decreased plasma E2 and elevated plasma DHT levels were also found. Thus, the dual functional 17β-HSD7 is proposed as a novel target for estrogen-dependent breast cancer by regulating the balance of E2 and DHT. This demonstrates a conceptual advance on the general belief that the major role of this enzyme is in cholesterol metabolism.
MeSH term(s)	17-Hydroxysteroid Dehydrogenases/antagonists & inhibitors ; 17-Hydroxysteroid Dehydrogenases/genetics ; 17-Hydroxysteroid Dehydrogenases/metabolism ; Androgens/blood ; Androgens/metabolism ; Androgens/pharmacology ; Apoptosis ; Apoptosis Regulatory Proteins/metabolism ; Breast Neoplasms/enzymology ; Breast Neoplasms/metabolism ; Cell Cycle Checkpoints ; Cell Proliferation/drug effects ; Cholesterol/metabolism ; Cyclin D1/metabolism ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Dihydrotestosterone/blood ; Dihydrotestosterone/metabolism ; Dihydrotestosterone/pharmacology ; Estradiol/blood ; Estradiol/metabolism ; Estradiol Dehydrogenases/chemistry ; Estrogens/blood ; Estrogens/metabolism ; Estrone/metabolism ; Female ; G1 Phase ; Humans ; MCF-7 Cells ; Membrane Proteins/metabolism ; Multifunctional Enzymes/antagonists & inhibitors ; Multifunctional Enzymes/chemistry ; Multifunctional Enzymes/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Resting Phase, Cell Cycle
Chemical Substances	Androgens ; Apoptosis Regulatory Proteins ; BCL2 protein, human ; BIK protein, human ; CCND1 protein, human ; Cyclin-Dependent Kinase Inhibitor p21 ; Estrogens ; Membrane Proteins ; Multifunctional Enzymes ; Proto-Oncogene Proteins c-bcl-2 ; Dihydrotestosterone (08J2K08A3Y) ; Cyclin D1 (136601-57-5) ; Estrone (2DI9HA706A) ; Estradiol (4TI98Z838E) ; Cholesterol (97C5T2UQ7J) ; 17-Hydroxysteroid Dehydrogenases (EC 1.1.-) ; 3 (or 17)-beta-hydroxysteroid dehydrogenase (EC 1.1.1.51) ; Estradiol Dehydrogenases (EC 1.1.1.62) ; HSD17B1 protein, human (EC 1.1.1.62)
Language	English
Publishing date	2015-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2500949-7
ISSN	1759-4685 ; 1674-2788
ISSN (online)	1759-4685
ISSN	1674-2788
DOI	10.1093/jmcb/mjv028
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Article ; Online: In vitro interactions between mammary fibroblasts (Hs 578Bst) and cancer epithelial cells (MCF-7) modulate aromatase, steroid sulfatase and 17β-hydroxysteroid dehydrogenases.

Wang, Xiaoqiang / Sang, Xiaoye / Diorio, Caroline / Lin, Sheng-Xiang / Doillon, Charles J

Molecular and cellular endocrinology

2015 Volume 412, Page(s) 339–348

Abstract: Our objectives were to investigate the interactions between mammary cancer epithelial cells (MCF-7) and stromal cells (Hs-578Bst) at the level of the expression and inhibition of steroidogenesis enzymes by using monolayer and three dimensional co-culture ...

Abstract	Our objectives were to investigate the interactions between mammary cancer epithelial cells (MCF-7) and stromal cells (Hs-578Bst) at the level of the expression and inhibition of steroidogenesis enzymes by using monolayer and three dimensional co-culture models. Expressions of steroidogenesis enzymes and E2/DHT conversions in co-cultured MCF-7 and Hs-578Bst cells as well as the effects of aromatase inhibitor combined to steroid sulfatase (STS) and 17β-hydroxysteroid dehydrogenases (17βHSDs) inhibitors were evaluated. 17β-HSD type 7 was mostly modulated in MCF-7 cells whereas aromatase was mostly regulated in Hs578Bst cells thereby increasing E2 conversion and MCF-7 cell growth. A combination of inhibitors toward aromatase, STS and 17β-HSD7, was found to be the most significant treatment in decreasing E2 and elevating DHT thus inhibiting MCF-7 cell proliferation and spheroid-like cancer cell aggregation in collagen gel. The interactions between those cells modulated E2 formation in paracrine/intracrine manners by synergistically regulating aromatase, 17β-HSD7 and STS. Among tumor-associated cells, stromal fibroblasts may participate in intratumoral E2 deposition; therefore promoting breast cancer cell growth.
MeSH term(s)	17-Hydroxysteroid Dehydrogenases/genetics ; 17-Hydroxysteroid Dehydrogenases/metabolism ; Aromatase/genetics ; Aromatase/metabolism ; Biosynthetic Pathways ; Coculture Techniques ; Epithelial Cells/enzymology ; Fibroblasts/enzymology ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Humans ; MCF-7 Cells ; Mammary Glands, Human/pathology ; Steryl-Sulfatase/genetics ; Steryl-Sulfatase/metabolism
Chemical Substances	17-Hydroxysteroid Dehydrogenases (EC 1.1.-) ; 3 (or 17)-beta-hydroxysteroid dehydrogenase (EC 1.1.1.51) ; Aromatase (EC 1.14.14.1) ; Steryl-Sulfatase (EC 3.1.6.2)
Language	English
Publishing date	2015-09-05
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2015.05.032
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Zs.A 1127: Show issues

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Article ; Online: Biological evaluation of a new family of aminosteroids that display a selective toxicity for various malignant cell lines.

Jegham, Hajer / Maltais, René / Roy, Jenny / Doillon, Charles / Poirier, Donald

Anti-cancer drugs

2012 Volume 23, Issue 8, Page(s) 803–814

Abstract: This study investigated the antineoplasic potential of a new family of aminosteroids. The antiproliferative activity of seven 5α-androstane-3α,17β-diol derivatives selected from a screening study was measured on nine cancerous cell lines (HL-60, K-562, ... ...

Abstract	This study investigated the antineoplasic potential of a new family of aminosteroids. The antiproliferative activity of seven 5α-androstane-3α,17β-diol derivatives selected from a screening study was measured on nine cancerous cell lines (HL-60, K-562, LNCaP, PC-3, Shionogi, MCF-7, MDA-MB-231, BT-20, and OVCAR-3) and two normal cell lines (peripheral blood lymphocytes and WI-38). The aminosteroids efficiently inhibited the cell growth of seven cancer cell lines [inhibitory concentration (IC(50)) values=0.2-6.4 µmol/l] and showed weak toxicity on normal cell lines. Two representative aminosteroids were tested and found to induce apoptosis and a G0/G1 cell cycle block in HL-60-treated cells, but not terminal myeloid differentiation. By a nuclear morphology analysis with fluorescence microscopy, typical apoptotic morphological changes were exhibited by treated cells. One aminosteroid tested in vivo (xenograft model) reduced the breast cancer (MCF-7 cells) tumor growth induced in nude mice. Furthermore, the information gathered suggests that this family of aminosteroids induced growth inhibition cells by arresting the cell cycle and triggering apoptosis.
MeSH term(s)	Androstane-3,17-diol/chemistry ; Androstane-3,17-diol/pharmacology ; Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Cell Line ; Cell Line, Tumor ; Female ; G1 Phase/drug effects ; HL-60 Cells ; Humans ; Inhibitory Concentration 50 ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Microscopy, Fluorescence ; Neoplasms/drug therapy ; Neoplasms/pathology ; Resting Phase, Cell Cycle/drug effects ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents ; Androstane-3,17-diol (25126-76-5)
Language	English
Publishing date	2012-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1065301-6
ISSN	1473-5741 ; 0959-4973
ISSN (online)	1473-5741
ISSN	0959-4973
DOI	10.1097/CAD.0b013e328351aa8c
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Zs.A 3125: Show issues

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Article: Skin replacement using collagen extracted from bovine hide.

Doillon, C J

Clinical materials

1992 Volume 9, Issue 3-4, Page(s) 189–193

Abstract: This paper reviews the use of biological sponge-shape matrices as dermal replacements in order to orient newly formed wound tissue. Sponge-shape matrices consist of a scaffold made of cross-linked collagen extracted from bovine hide. Other molecules ... ...

Abstract	This paper reviews the use of biological sponge-shape matrices as dermal replacements in order to orient newly formed wound tissue. Sponge-shape matrices consist of a scaffold made of cross-linked collagen extracted from bovine hide. Other molecules with specific activities on wound tissue ingrowth are bound to collagen. The lamination of sponge with a synthetic material allows this device to be implanted as a temporary skin substitute. For the epidermal cell layer replacement, a biological film-shape matrix can be used in order to cultivate autologous cells during the period that biological sponge-shape matrices are invaded by wound tissue.
MeSH term(s)	Animals ; Biological Dressings ; Cattle ; Collagen/pharmacology ; Cross-Linking Reagents/pharmacology ; Cross-Linking Reagents/therapeutic use ; Humans ; Regeneration/drug effects ; Wound Healing/drug effects
Chemical Substances	Cross-Linking Reagents ; Collagen (9007-34-5)
Language	English
Publishing date	1992
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	639229-5
ISSN	1878-6979 ; 0267-6605
ISSN (online)	1878-6979
ISSN	0267-6605
DOI	10.1016/0267-6605(92)90099-f
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Zs.B 3148: Show issues

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Article ; Online: The stimulation of angiogenesis and collagen deposition by copper.

Gérard, Catherine / Bordeleau, Louis-Jean / Barralet, Jake / Doillon, Charles J

Biomaterials

2010 Volume 31, Issue 5, Page(s) 824–831

Abstract: Copper is known to trigger endothelial cells towards angiogenesis. Different approaches have been investigated to develop vascularisation in biomaterials. The angiogenic and healing potential of copper ions in combination with two major angiogenic ... ...

Abstract	Copper is known to trigger endothelial cells towards angiogenesis. Different approaches have been investigated to develop vascularisation in biomaterials. The angiogenic and healing potential of copper ions in combination with two major angiogenic factors was examined. A 3D culture system in which, under stimulation by FGF-2 and to a lesser degree with VEGF, endothelial cells assembled into structures resembling to an angiogenic process was used. The combination of CuSO(4) with increasing doses of VEGF or FGF-2 enhanced the complexity of angiogenic networks in a significant manner. In vivo studies were also conducted by incorporating FGF-2 with CuSO(4) in a cylindrical collagen-based scaffold. CuSO(4) enhanced significantly the invasion of microvessel compared to control implants and to 20ng FGF-2+/-CuSO(4). Vascular infiltration was also significantly improved by combination of CuSO(4) with FGF-2, compared to FGF-2 alone (0.2 and 1microg). Nevertheless, in comparison with CuSO(4) alone, there was a significant increase only with 1microg of FGF-2 combined with CuSO(4). Significantly, collagen fiber deposition was enhanced following the combinatory loading in comparison to that with FGF-2 alone but not with CuSO(4) only. Thus, copper associated with growth factors may have synergistic effects which are highly attractive in the fields of tissue engineering (e.g., bone) and biomaterials.
MeSH term(s)	Biocompatible Materials/administration & dosage ; Biocompatible Materials/chemistry ; Cell Culture Techniques/methods ; Cell Proliferation/drug effects ; Cells, Cultured ; Collagen/administration & dosage ; Collagen/chemistry ; Copper/administration & dosage ; Copper/chemistry ; Endothelial Cells/drug effects ; Endothelial Cells/physiology ; Humans ; Neovascularization, Physiologic/drug effects ; Neovascularization, Physiologic/physiology ; Tissue Engineering/methods
Chemical Substances	Biocompatible Materials ; Copper (789U1901C5) ; Collagen (9007-34-5)
Language	English
Publishing date	2010-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	603079-8
ISSN	1878-5905 ; 0142-9612
ISSN (online)	1878-5905
ISSN	0142-9612
DOI	10.1016/j.biomaterials.2009.10.009
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Zs.B 2371: Show issues

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Article: Porous collagen sponge wound dressings: in vivo and in vitro studies.

Doillon, C J

Journal of biomaterials applications

1988 Volume 2, Issue 4, Page(s) 562–578

Abstract: Collagen-based materials can be formed into a three-dimensional sponge for use as a wound dressing and as a support for cell cultured skin components. Factors such as biocompatibility, morphological structure and addition of non-collagenous molecules to ... ...

Abstract	Collagen-based materials can be formed into a three-dimensional sponge for use as a wound dressing and as a support for cell cultured skin components. Factors such as biocompatibility, morphological structure and addition of non-collagenous molecules to collagen are analyzed and discussed. Large pores or channels, interchannel communications and combinations of macromolecules of the connective tissue enhance wound tissue infiltration in vivo as well as cell growth in vitro into collagen sponges. The presence of such factors can be useful in patients with excised burn wounds and pressure skin ulcers.
MeSH term(s)	Animals ; Bandages ; Biological Dressings ; Collagen ; Humans ; In Vitro Techniques ; Materials Testing ; Wound Healing
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	1988-04
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	639283-0
ISSN	0885-3282
ISSN	0885-3282
DOI	10.1177/088532828700200404
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Zs.B 3175: Show issues

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Article ; Online: Adipogenesis in nonadherent and adherent bone marrow stem cells grown in fibrin gel and in the presence of adult plasma.

Gérard, Catherine / Blouin, Karine / Tchernof, André / Doillon, Charles J

Cells, tissues, organs

2008 Volume 187, Issue 3, Page(s) 186–198

Abstract: Bone marrow-derived mesenchymal stem cells (i.e., adherent cells) are known to differentiate into fat tissue in the presence of adipogenic supplements in cultures. Induction of adipogenesis has not been investigated within the nonadherent cell fraction ... ...

Abstract	Bone marrow-derived mesenchymal stem cells (i.e., adherent cells) are known to differentiate into fat tissue in the presence of adipogenic supplements in cultures. Induction of adipogenesis has not been investigated within the nonadherent cell fraction that includes predominantly hematopoietic cells. In the present study, murine nonadherent bone marrow-derived stem cells (96% CD45+ cells) were seeded and then grown in fibrin gel to form cell clusters in which most cells were positive to DiI-acetylated low-density lipoprotein uptake. Amongst different culture media supplemented either in fetal bovine serum, horse serum, murine plasma, human plasma or adipogenic supplements, a subpopulation of nonadherent stem cells within clusters differentiated into adipocytes, specifically in the presence of adult syngeneic plasma. This was confirmed by the observation and quantification of oil red O-positive cells, the measurement of glycerol-3-phosphate dehydrogenase activity and peroxisome proliferator-activated receptor-gamma mRNA expression. Similarly, adipogenesis was also observed in the presence of murine plasma with adherent mesenchymal stem cells and 3T3-L1 preadipocytes which were grown either in monolayer plastic cultures or in fibrin gel. Thus, it is possible that nonadherent cells, once in a 3-dimensional environment, can further differentiate towards adipogenesis.
MeSH term(s)	Adipogenesis/drug effects ; Adipogenesis/physiology ; Adipose Tissue/cytology ; Adult ; Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/drug effects ; Cell Adhesion/drug effects ; Cell Adhesion/physiology ; Cell Culture Techniques ; Cell Differentiation/physiology ; Cells, Cultured ; Culture Media/chemistry ; Culture Media/pharmacology ; Fibrin/chemistry ; Fibrin/pharmacology ; Gels/chemistry ; Gels/pharmacology ; Glycerolphosphate Dehydrogenase/metabolism ; Humans ; Mice ; PPAR gamma/metabolism ; Plasma/chemistry ; Plasma/metabolism ; Staining and Labeling ; Stem Cells/cytology
Chemical Substances	Culture Media ; Gels ; PPAR gamma ; Fibrin (9001-31-4) ; Glycerolphosphate Dehydrogenase (EC 1.1.-)
Language	English
Publishing date	2008
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1468141-9
ISSN	1422-6421 ; 1422-6405
ISSN (online)	1422-6421
ISSN	1422-6405
DOI	10.1159/000111804
Database	MEDical Literature Analysis and Retrieval System OnLINE

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