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Article ; Online: Predicting suicidal events: A comparison of the Concise Health Risk Tracking Self-Report (CHRT-SR) and the Columbia Suicide Severity Rating Scale (C-SSRS).

Mayes, Taryn L / Carmody, Thomas / Rush, A John / Nandy, Karabi / Emslie, Graham J / Kennard, Beth D / Forbes, Kathryn / Jha, Manish K / Hughes, Jennifer L / Heerschap, Jessica K / Trivedi, Madhukar H

Psychiatry research

2023 Volume 326, Page(s) 115306

Abstract: This report examines the predictive capabilities of two scales of suicidality in high-risk adolescents. Charts of adolescents with severe suicidality participating in an intensive outpatient program were reviewed. Self-report data from the 9-item Concise ...

Abstract	This report examines the predictive capabilities of two scales of suicidality in high-risk adolescents. Charts of adolescents with severe suicidality participating in an intensive outpatient program were reviewed. Self-report data from the 9-item Concise Health Risk Tracking Self-Report (CHRT-SR
MeSH term(s)	Adolescent ; Humans ; Suicidal Ideation ; Self Report ; Psychometrics ; Suicide, Attempted ; Reproducibility of Results ; Psychiatric Status Rating Scales
Language	English
Publishing date	2023-06-21
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	445361-x
ISSN	1872-7123 ; 1872-7506 ; 0925-4927 ; 0165-1781
ISSN (online)	1872-7123 ; 1872-7506
ISSN	0925-4927 ; 0165-1781
DOI	10.1016/j.psychres.2023.115306
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Article ; Online: C.DOT - Convolutional Deep Object Tracker for Augmented Reality Based Purely on Synthetic Data.

Thiel, Kevin Kennard / Naumann, Florian / Jundt, Eduard / Gunnemann, Stephan / Klinker, Gudrun

IEEE transactions on visualization and computer graphics

2022 Volume 28, Issue 12, Page(s) 4434–4451

Abstract: Augmented reality applications use object tracking to estimate the pose of a camera and to superimpose virtual content onto the observed object. Today, a number of tracking systems are available, ready to be used in industrial applications. However, such ...

Abstract	Augmented reality applications use object tracking to estimate the pose of a camera and to superimpose virtual content onto the observed object. Today, a number of tracking systems are available, ready to be used in industrial applications. However, such systems are hard to handle for a service maintenance engineer, due to obscure configuration procedures. In this article, we investigate options towards replacing the manual configuration process with a machine learning approach based on automatically synthesized data. We present an automated process of creating object tracker facilities exclusively from synthetic data. The data is highly enhanced to train a convolutional neural network, while still being able to receive reliable and robust results during real world applications only from simple RGB cameras. Comparison against related work using the LINEMOD dataset showed that we are able to outperform similar approaches. For our intended industrial applications with high accuracy demands, its performance is still lower than common object tracking methods with manual configuration. Yet, it can greatly support those as an add-on during initialization, due to its higher reliability.
Language	English
Publishing date	2022-10-26
Publishing country	United States
Document type	Journal Article
ISSN	1941-0506
ISSN (online)	1941-0506
DOI	10.1109/TVCG.2021.3089096
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Article ; Online: HIV Protease Inhibitor Ritonavir Impairs Endothelial Function Via Reduction in Adipose Mass and Endothelial Leptin Receptor-Dependent Increases in NADPH Oxidase 1 (Nox1), C-C Chemokine Receptor Type 5 (CCR5), and Inflammation.

Bruder-Nascimento, Thiago / Kress, Taylor C / Kennard, Simone / Belin de Chantemèle, Eric J

Journal of the American Heart Association

2020 Volume 9, Issue 19, Page(s) e018074

Abstract: Background Cardiovascular disease is currently the leading cause of death in patients with human immunodeficiency virus on combination antiretroviral therapy. Although the use of the protease inhibitor ritonavir has been associated with increased ... ...

Abstract	Background Cardiovascular disease is currently the leading cause of death in patients with human immunodeficiency virus on combination antiretroviral therapy. Although the use of the protease inhibitor ritonavir has been associated with increased prevalence of cardiovascular disease, the underlying mechanisms remain ill-defined. Herein, we tested the hypothesis that ritonavir-mediated lipoatrophy causes endothelial dysfunction via reducing endothelial leptin signaling. Methods and Results Long-term (4 weeks) but not short-term (3 days) treatment with ritonavir reduced body weight, fat mass, and leptin levels and induced endothelial dysfunction in mice. Moreover, ritonavir increased vascular NADPH oxidase 1, aortic H
MeSH term(s)	Adipose Tissue/drug effects ; Adipose Tissue/metabolism ; Animals ; Body Weight/drug effects ; Cardiovascular Diseases/etiology ; Cardiovascular Diseases/metabolism ; Cardiovascular Diseases/prevention & control ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; HIV Protease Inhibitors/pharmacokinetics ; Inflammation/metabolism ; Leptin/metabolism ; Mice ; NADPH Oxidase 1/metabolism ; Oxidative Stress/drug effects ; Receptors, CCR5/metabolism ; Receptors, Leptin/metabolism ; Ritonavir/pharmacokinetics ; Signal Transduction
Chemical Substances	CCR5 protein, mouse ; HIV Protease Inhibitors ; Leptin ; Receptors, CCR5 ; Receptors, Leptin ; leptin receptor, mouse ; NADPH Oxidase 1 (EC 1.6.3.-) ; NOX1 protein, mouse (EC 1.6.3.-) ; Ritonavir (O3J8G9O825)
Language	English
Publishing date	2020-10-02
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2653953-6
ISSN	2047-9980 ; 2047-9980
ISSN (online)	2047-9980
ISSN	2047-9980
DOI	10.1161/JAHA.120.018074
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Article ; Online: Dietary sodium restriction sex specifically impairs endothelial function via mineralocorticoid receptor-dependent reduction in NO bioavailability in Balb/C mice.

Faulkner, Jessica L / Harwood, Daisy / Kennard, Simone / Antonova, Galina / Clere, Nicolas / Belin de Chantemèle, Eric J

American journal of physiology. Heart and circulatory physiology

2020 Volume 320, Issue 1, Page(s) H211–H220

Abstract: ... impairs endothelial function in otherwise healthy female mice. We fed male and female Balb/C mice a normal ...

Abstract	Recent findings from our group demonstrated that females exhibit higher endothelial mineralocorticoid receptor (MR) expression than males, which predisposes them to aldosterone-mediated endothelial dysfunction in the context of metabolic disorders. However, whether the endothelium of female mice presents a higher propensity to MR-mediated dysfunction than that of males in the absence of comorbidities remains unknown. We therefore sought to investigate whether increasing aldosterone production endogenously with sodium restriction impairs endothelial function in otherwise healthy female mice. We fed male and female Balb/C mice a normal (0.4% NaCl; NSD) or sodium-restricted diet (0.05% NaCl; SRD) for 4 wk. Females exhibited higher baseline endothelial function (relaxation to acetylcholine) and lower vascular contractility (constriction to phenylephrine, serotonin, and KCl). However, SRD impaired endothelial-dependent relaxation and increased vascular contractility in female mice, effectively ablating the baseline sex difference. Female sex also increased baseline adrenal
MeSH term(s)	Adrenal Glands/enzymology ; Aldosterone/blood ; Animals ; Cytochrome P-450 CYP11B2/metabolism ; Diet, Sodium-Restricted ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiopathology ; Female ; Male ; Mice, Inbred BALB C ; NADPH Oxidase 4/metabolism ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type III/metabolism ; Receptor, Angiotensin, Type 1/metabolism ; Receptors, Mineralocorticoid/metabolism ; Sex Factors ; Signal Transduction ; Up-Regulation ; Vasoconstriction ; Vasodilation ; Mice
Chemical Substances	Receptor, Angiotensin, Type 1 ; Receptors, Mineralocorticoid ; Nitric Oxide (31C4KY9ESH) ; Aldosterone (4964P6T9RB) ; Nitric Oxide Synthase Type III (EC 1.14.13.39) ; Nos3 protein, mouse (EC 1.14.13.39) ; Cytochrome P-450 CYP11B2 (EC 1.14.15.4) ; NADPH Oxidase 4 (EC 1.6.3.-) ; Nox4 protein, mouse (EC 1.6.3.-)
Language	English
Publishing date	2020-10-23
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	603838-4
ISSN	1522-1539 ; 0363-6135
ISSN (online)	1522-1539
ISSN	0363-6135
DOI	10.1152/ajpheart.00413.2020
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Article ; Online: Lack of Suppression of Aldosterone Production Leads to Salt-Sensitive Hypertension in Female but Not Male Balb/C Mice.

Faulkner, Jessica L / Harwood, Daisy / Bender, Lily / Shrestha, Lenee / Brands, Michael W / Morwitzer, M Jane / Kennard, Simone / Antonova, Galina / Belin de Chantemèle, Eric J

Hypertension (Dallas, Tex. : 1979)

2018 Volume 72, Issue 6, Page(s) 1397–1406

Abstract: ... therefore, elucidation of these mechanisms in female animal models is needed. We have previously shown that female Balb/C ... that female Balb/C mice develop salt-sensitive increases in blood pressure. Seven-day feeding of a 4% NaCl ... blood pressure and endothelial function in females on an HS diet. Collectively, these data indicate that Balb/C ...

Abstract	Clinical studies indicate that salt-sensitive hypertension is more prevalent in women than in men. However, animal models of salt sensitivity have primarily focused on the mechanisms of salt sensitivity in male animals; therefore, elucidation of these mechanisms in female animal models is needed. We have previously shown that female Balb/C mice have higher aldosterone synthase expression and aldosterone production than males. We hypothesized that female Balb/C mice develop salt-sensitive increases in blood pressure. Seven-day feeding of a 4% NaCl high-salt (HS) diet increased blood pressure in female mice without altering blood pressure in males. Females on an HS diet displayed no apparent increases in sodium retention as assessed by 24-hour urine collection, sodium balance measure, and saline loading excretion analysis. Females on an HS diet exhibited lower renin-angiotensin system activity (plasma Ang II [angiotensin II], plasma renin activity, and ACE [angiotensin-converting enzyme] activity) compared with males but developed a salt-induced elevation in adrenal aldosterone synthase expression and retained higher aldosterone levels than males on HS. This resulted in a higher aldosterone/plasma renin activity ratio in females compared with males on HS feeding. Adrenal mRNA expression of angiotensinogen and leptin receptor was increased in female mice on an HS diet. HS impaired endothelium-dependent relaxation in female mice only. MR (mineralocorticoid receptor) inhibition (eplerenone) restored blood pressure and endothelial function in females on an HS diet. Collectively, these data indicate that Balb/C mice develop sex-discrepant salt-sensitive hypertension likely via aldosterone-MR-mediated mechanisms involving impaired endothelium-dependent relaxation in females only. This study presents the first model of spontaneous sex-specific salt sensitivity, which mimics the human pathology.
MeSH term(s)	Adrenal Glands/metabolism ; Aldosterone/metabolism ; Angiotensinogen/metabolism ; Animals ; Blood Pressure/physiology ; Female ; Hypertension/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Receptors, Leptin/metabolism ; Renin-Angiotensin System/physiology ; Sex Factors ; Sodium Chloride, Dietary
Chemical Substances	Receptors, Leptin ; Sodium Chloride, Dietary ; Angiotensinogen (11002-13-4) ; Aldosterone (4964P6T9RB)
Language	English
Publishing date	2018-11-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	423736-5
ISSN	1524-4563 ; 0194-911X ; 0362-4323
ISSN (online)	1524-4563
ISSN	0194-911X ; 0362-4323
DOI	10.1161/HYPERTENSIONAHA.118.11303
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Zs.A 1488: Show issues

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Article ; Online: Calcineurin-mediated dephosphorylation of eNOS at serine 116 affects eNOS enzymatic activity indirectly by facilitating c-Src binding and tyrosine 83 phosphorylation.

Ruan, Ling / Torres, Christina M / Buffett, Ryan J / Kennard, Simone / Fulton, David / Venema, Richard C

Vascular pharmacology

2013 Volume 59, Issue 1-2, Page(s) 27–35

Abstract: ... however, does interfere with the interaction of eNOS with c-Src, an interaction which is known to activate eNOS ... by facilitating c-Src binding and Y83 phosphorylation. ...

Abstract	It has been shown previously that phosphorylation of the endothelial nitric oxide synthase (eNOS) at serine 116 (S116) under basal conditions suppresses eNOS enzymatic activity in endothelial cells. It has also been shown that vascular endothelial growth factor (VEGF) treatment of endothelial cells produces a rapid S116 dephosphorylation, which is blocked by the calcineurin inhibitor, cyclosporin A (CsA). In this study, we show that activation of eNOS in response to a variety of other eNOS-activating agonists and the cytosolic calcium-elevating agent, thapsigargin also involves CsA-inhibitable S116 dephosphorylation. Studies with the purified eNOS enzyme also demonstrate that neither mimicking phosphorylation at S116 nor phosphorylation of the purified enzyme at S116 in vitro has any effect on enzymatic activity. Phospho-mimicking, however, does interfere with the interaction of eNOS with c-Src, an interaction which is known to activate eNOS by phosphorylation at tyrosine 83 (Y83). Agonist-stimulated eNOS-Src complex formation, as well as agonist-stimulated Y83 phosphorylation, are blocked by calcineurin inhibition by CsA and by a cell-permeable calcineurin inhibitory peptide. Taken together, these data suggest a mechanism of eNOS regulation whereby calcineurin-mediated dephosphorylation of eNOS at S116 affects eNOS enzymatic activity indirectly, rather than directly, by facilitating c-Src binding and Y83 phosphorylation.
MeSH term(s)	Animals ; COS Cells ; Calcineurin/metabolism ; Calcium/metabolism ; Cattle ; Cyclosporine/pharmacology ; Endothelial Cells/drug effects ; Endothelial Cells/enzymology ; Endothelial Cells/metabolism ; Endothelium, Vascular/drug effects ; Endothelium, Vascular/enzymology ; Endothelium, Vascular/metabolism ; Enzyme Inhibitors/pharmacology ; Nitric Oxide Synthase Type III/antagonists & inhibitors ; Nitric Oxide Synthase Type III/metabolism ; Permeability/drug effects ; Phosphorylation ; Serine/metabolism ; Thapsigargin/pharmacology ; Tyrosine/metabolism
Chemical Substances	Enzyme Inhibitors ; Tyrosine (42HK56048U) ; Serine (452VLY9402) ; Thapsigargin (67526-95-8) ; Cyclosporine (83HN0GTJ6D) ; Nitric Oxide Synthase Type III (EC 1.14.13.39) ; Calcineurin (EC 3.1.3.16) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2013-05-30
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2082846-9
ISSN	1879-3649 ; 1537-1891 ; 1879-3649
ISSN (online)	1879-3649 ; 1537-1891
ISSN	1879-3649
DOI	10.1016/j.vph.2013.05.004
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Zs.A 591: Show issues

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Article ; Online: A Retrospective Review of Pregnancies in our Psoriasis Biologics Cohort.

Wolfe, Charlotte / Kennard, Benjamin / Bewley, Anthony / Mehrtens, Sarah

Clinical and experimental dermatology

2024

Language	English
Publishing date	2024-03-25
Publishing country	England
Document type	Journal Article
ZDB-ID	195504-4
ISSN	1365-2230 ; 0307-6938
ISSN (online)	1365-2230
ISSN	0307-6938
DOI	10.1093/ced/llae106
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Article ; Online: Vitamin C deficiency in the brain impairs cognition, increases amyloid accumulation and deposition, and oxidative stress in APP/PSEN1 and normally aging mice.

Dixit, Shilpy / Bernardo, Alexandra / Walker, Jennifer Michelle / Kennard, John Andrew / Kim, Grace Youngeun / Kessler, Eric Sean / Harrison, Fiona Edith

ACS chemical neuroscience

2015 Volume 6, Issue 4, Page(s) 570–581

Abstract: Subclinical vitamin C deficiency is widespread in many populations, but its role ... in both Alzheimer's disease and normal aging is understudied. In the present study, we decreased brain vitamin C in the APPSWE ... heterozygous knockout mice, which have lower numbers of the sodium-dependent vitamin C transporter required ...

Abstract	Subclinical vitamin C deficiency is widespread in many populations, but its role in both Alzheimer's disease and normal aging is understudied. In the present study, we decreased brain vitamin C in the APPSWE/PSEN1deltaE9 mouse model of Alzheimer's disease by crossing APP/PSEN1(+) bigenic mice with SVCT2(+/-) heterozygous knockout mice, which have lower numbers of the sodium-dependent vitamin C transporter required for neuronal vitamin C transport. SVCT2(+/-) mice performed less well on the rotarod task at both 5 and 12 months of age compared to littermates. SVCT2(+/-) and APP/PSEN1(+) mice and the combination genotype SVCT2(+/-)APP/PSEN1(+) were also impaired on multiple tests of cognitive ability (olfactory memory task, Y-maze alternation, conditioned fear, Morris water maze). In younger mice, both low vitamin C (SVCT2(+/-)) and APP/PSEN1 mutations increased brain cortex oxidative stress (malondialdehyde, protein carbonyls, F2-isoprostanes) and decreased total glutathione compared to wild-type controls. SVCT2(+/-) mice also had increased amounts of both soluble and insoluble Aβ1-42 and a higher Aβ1-42/1-40 ratio. By 14 months of age, oxidative stress levels were similar among groups, but there were more amyloid-β plaque deposits in both hippocampus and cortex of SVCT2(+/-)APP/PSEN1(+) mice compared to APP/PSEN1(+) mice with normal brain vitamin C. These data suggest that even moderate intracellular vitamin C deficiency plays an important role in accelerating amyloid pathogenesis, particularly during early stages of disease development, and that these effects are likely modulated by oxidative stress pathways.
MeSH term(s)	Aging/metabolism ; Aging/pathology ; Alzheimer Disease/metabolism ; Alzheimer Disease/pathology ; Amyloid beta-Peptides/metabolism ; Amyloid beta-Protein Precursor/genetics ; Amyloid beta-Protein Precursor/metabolism ; Animals ; Anxiety/metabolism ; Anxiety/pathology ; Ascorbic Acid/metabolism ; Ascorbic Acid Deficiency/metabolism ; Ascorbic Acid Deficiency/pathology ; Ascorbic Acid Deficiency/psychology ; Brain/metabolism ; Brain/pathology ; Cognition Disorders/metabolism ; Cognition Disorders/pathology ; Disease Models, Animal ; Female ; Learning/physiology ; Male ; Memory/physiology ; Mice, Transgenic ; Motor Activity/physiology ; Oxidative Stress/physiology ; Peptide Fragments/metabolism ; Presenilin-1/genetics ; Presenilin-1/metabolism ; Sodium-Coupled Vitamin C Transporters/genetics ; Sodium-Coupled Vitamin C Transporters/metabolism
Chemical Substances	APP protein, human ; Amyloid beta-Peptides ; Amyloid beta-Protein Precursor ; PSEN1 protein, human ; Peptide Fragments ; Presenilin-1 ; Slc23a2 protein, mouse ; Sodium-Coupled Vitamin C Transporters ; amyloid beta-protein (1-40) ; amyloid beta-protein (1-42) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2015-04-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1948-7193
ISSN (online)	1948-7193
DOI	10.1021/cn500308h
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Article: Vitamin C Concentration in Developing and Mature Fruits of Mango (Mangifera indica L.).

Spencer, J L / Morris, M P / Kennard, W C

Plant physiology

2006 Volume 31, Issue 1, Page(s) 79–80

Language	English
Publishing date	2006-04-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	208914-2
ISSN	1532-2548 ; 0032-0889
ISSN (online)	1532-2548
ISSN	0032-0889
DOI	10.1104/pp.31.1.79
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Article ; Online: The response threshold of Salmonella PilZ domain proteins is determined by their binding affinities for c-di-GMP.

Pultz, Ingrid Swanson / Christen, Matthias / Kulasekara, Hemantha Don / Kennard, Andrew / Kulasekara, Bridget / Miller, Samuel I

Molecular microbiology

2012 Volume 86, Issue 6, Page(s) 1424–1440

Abstract: c-di-GMP is a bacterial second messenger that is enzymatically synthesized and degraded in response ... to environmental signals. Cellular processes are affected when c-di-GMP binds to receptors which include proteins ... that contain the PilZ domain. Although each c-di-GMP synthesis or degradation enzyme metabolizes the same ...

Abstract	c-di-GMP is a bacterial second messenger that is enzymatically synthesized and degraded in response to environmental signals. Cellular processes are affected when c-di-GMP binds to receptors which include proteins that contain the PilZ domain. Although each c-di-GMP synthesis or degradation enzyme metabolizes the same molecule, many of these enzymes can be linked to specific downstream processes. Here we present evidence that c-di-GMP signalling specificity is achieved through differences in affinities of receptor macromolecules. We show that the PilZ domain proteins of Salmonella Typhimurium, YcgR and BcsA, demonstrate a 43-fold difference in their affinity for c-di-GMP. Modulation of the affinities of these proteins altered their activities in a predictable manner in vivo. Inactivation of yhjH, which encodes a predicted c-di-GMP degrading enzyme, increased the fraction of the cellular population that demonstrated c-di-GMP levels high enough to bind to the higher-affinity YcgR protein and inhibit motility, but not high enough to bind to the lower-affinity BcsA protein and stimulate cellulose production. Finally, PilZ domain proteins of Pseudomonas aeruginosa demonstrated a 145-fold difference in binding affinities, suggesting that regulation by binding affinity may be a conserved mechanism that allows organisms with many c-di-GMP binding macromolecules to rapidly integrate multiple environmental signals into one output.
MeSH term(s)	Bacterial Proteins/metabolism ; Cellulose/metabolism ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/metabolism ; Gene Expression Regulation, Bacterial ; Locomotion ; Protein Binding ; Salmonella typhimurium/genetics ; Salmonella typhimurium/metabolism ; Salmonella typhimurium/physiology ; Signal Transduction
Chemical Substances	Bacterial Proteins ; bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Cellulose (9004-34-6) ; Cyclic GMP (H2D2X058MU)
Language	English
Publishing date	2012-11-19
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	619315-8
ISSN	1365-2958 ; 0950-382X
ISSN (online)	1365-2958
ISSN	0950-382X
DOI	10.1111/mmi.12066
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