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Article ; Online: Fluorescence-based lateral flow assays for rapid oral fluid roadside detection of cannabis use.

Plouffe, Brian D / Murthy, Shashi K

2017 Volume 38, Issue 3-4, Page(s) 501–506

Abstract: With the recent worldwide changes in the legalization of marijuana, there is a significant need for rapid, roadside screening test for driving under the influence of drugs. A robust, sensitive, lateral flow assay has been developed to detect recent use ... ...

Abstract	With the recent worldwide changes in the legalization of marijuana, there is a significant need for rapid, roadside screening test for driving under the influence of drugs. A robust, sensitive, lateral flow assay has been developed to detect recent use via oral-fluid testing for Δ
MeSH term(s)	Automobile Driving ; Cannabis ; Dronabinol/analysis ; Fluoroimmunoassay/methods ; Humans ; Limit of Detection ; Linear Models ; Marijuana Smoking ; Microfluidic Analytical Techniques/methods ; Reproducibility of Results ; Saliva/chemistry ; Substance Abuse Detection/methods
Chemical Substances	Dronabinol (7J8897W37S)
Language	English
Publishing date	2017-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	619001-7
ISSN	1522-2683 ; 0173-0835
ISSN (online)	1522-2683
ISSN	0173-0835
DOI	10.1002/elps.201600075
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Article ; Online: Perspective on microfluidic cell separation: a solved problem?

Plouffe, Brian D / Murthy, Shashi K

Analytical chemistry

2014 Volume 86, Issue 23, Page(s) 11481–11488

Abstract: The purification and sorting of cells using microfluidic methodologies has been a remarkably active area of research over the past decade. Much of the scientific and technological work associated with microfluidic cell separation has been driven by needs ...

Abstract	The purification and sorting of cells using microfluidic methodologies has been a remarkably active area of research over the past decade. Much of the scientific and technological work associated with microfluidic cell separation has been driven by needs in clinical diagnostics and therapeutic monitoring, most notably in the context of circulating tumor cells. The last several years have seen advances in a broad range of separation modalities ranging from miniaturized analogs of established techniques such as fluorescence- and magnetic-activated cell sorting (FACS and MACS, respectively), to more specialized approaches based on affinity, dielectrophoretic mobility, and inertial properties of cells. With several of these technologies nearing commercialization, there is a sense that the field of microfluidic cell separation has achieved a high level of maturity over an unusually short span of time. In this Perspective, we set the stage by describing major scientific and technological advances in this field and ask what the future holds. While many scientific questions remain unanswered and new compelling questions will undoubtedly arise, the relative maturity of this field poses some unique challenges.
MeSH term(s)	Cell Separation ; Microfluidic Analytical Techniques
Language	English
Publishing date	2014-11-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac5013283
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Article: Fluorescenceâbased lateral flow assays for rapid oral fluid roadside detection of cannabis use

Plouffe, Brian D / Shashi K. Murthy

Electrophoresis. 2017 Feb., v. 38, no. 3-4

2017

Abstract	With the recent worldwide changes in the legalization of marijuana, there is a significant need for rapid, roadside screening test for driving under the influence of drugs. A robust, sensitive, lateral flow assay has been developed to detect recent use via oralâfluid testing for Îâ¹âtetrahydrocannabinol (THC). This proofâofâconcept assay uses a fluorescentâbased immunoassay detection of polymeric beads, conjugated to antibodies against native THC. The fluorescent technique allows for significantly lower limits of detection and higher precision determination of recent marijuana use without the use of urine or blood samplingâthus allowing for roadside identification. Detection levels of 0.01 ng/mL were distinguished from background and the lower limit of quantification was determined to approach 1 ng/mL.
Keywords	Cannabis sativa ; antibodies ; blood ; detection limit ; drugs ; electrophoresis ; fluorescence ; immunoassays ; screening ; tetrahydrocannabinol ; urine
Language	English
Dates of publication	2017-02
Size	p. 501-506.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	619001-7
ISSN	1522-2683 ; 0173-0835
ISSN (online)	1522-2683
ISSN	0173-0835
DOI	10.1002/elps.201600075
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Article ; Online: Circulating Endothelial Cell Quantification by Microfluidics Chip in Pulmonary Arterial Hypertension.

Sallmon, Hannes / Hatch, Adam / Murthy, Shashi K / Plouffe, Brian D / Hansmann, Georg

American journal of respiratory cell and molecular biology

2017 Volume 56, Issue 5, Page(s) 680–682

Language	English
Publishing date	2017-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	1025960-0
ISSN	1535-4989 ; 1044-1549
ISSN (online)	1535-4989
ISSN	1044-1549
DOI	10.1165/rcmb.2017-0026LE
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Article ; Online: Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.

Plouffe, Brian D / Murthy, Shashi K / Lewis, Laura H

Reports on progress in physics. Physical Society (Great Britain)

2014 Volume 78, Issue 1, Page(s) 16601

Abstract: Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a ... ...

Abstract	Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell-separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell-separation systems.
MeSH term(s)	Animals ; Cell Separation/economics ; Cell Separation/instrumentation ; Cell Separation/methods ; Humans ; Magnetic Phenomena ; Magnets
Language	English
Publishing date	2014-12-04
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	205657-4
ISSN	1361-6633 ; 0034-4885
ISSN (online)	1361-6633
ISSN	0034-4885
DOI	10.1088/0034-4885/78/1/016601
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Article ; Online: Soluble Receptor for Advanced Glycation End Products (sRAGE) Is a Sensitive Biomarker in Human Pulmonary Arterial Hypertension.

Diekmann, Franziska / Chouvarine, Philippe / Sallmon, Hannes / Meyer-Kobbe, Louisa / Kieslich, Moritz / Plouffe, Brian D / Murthy, Shashi K / Lichtinghagen, Ralf / Legchenko, Ekaterina / Hansmann, Georg

International journal of molecular sciences

2021 Volume 22, Issue 16

Abstract: Pulmonary arterial hypertension (PAH) is a progressive condition with an unmet need for early diagnosis, better monitoring, and risk stratification. The receptor for advanced glycation end products (RAGE) is activated in response to hypoxia and vascular ... ...

Abstract	Pulmonary arterial hypertension (PAH) is a progressive condition with an unmet need for early diagnosis, better monitoring, and risk stratification. The receptor for advanced glycation end products (RAGE) is activated in response to hypoxia and vascular injury, and is associated with inflammation, cell proliferation and migration in PAH. For the adult cohort, we recruited 120 patients with PAH, 83 with idiopathic PAH (IPAH) and 37 with connective tissue disease-associated PAH (CTD-PAH), and 48 controls, and determined potential plasma biomarkers by enzyme-linked immunoassay. The established heart failure marker NTproBNP and IL-6 plasma levels were several-fold higher in both adult IPAH and CTD-PAH patients versus controls. Plasma soluble RAGE (sRAGE) was elevated in IPAH patients (3044 ± 215.2 pg/mL) and was even higher in CTD-PAH patients (3332 ± 321.6 pg/mL) versus controls (1766 ± 121.9 pg/mL;
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Biomarkers/blood ; Case-Control Studies ; Female ; Germany ; Humans ; Male ; Middle Aged ; Prognosis ; Pulmonary Arterial Hypertension/blood ; Pulmonary Arterial Hypertension/diagnosis ; Receptor for Advanced Glycation End Products/blood ; Sensitivity and Specificity ; Solubility ; Young Adult
Chemical Substances	Biomarkers ; Receptor for Advanced Glycation End Products
Language	English
Publishing date	2021-08-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22168591
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Article: Perspective on Microfluidic Cell Separation: A Solved Problem?

Plouffe, Brian D / Murthy Shashi K

Analytical chemistry. 2014 Dec. 02, v. 86, no. 23

2014

Abstract	The purification and sorting of cells using microfluidic methodologies has been a remarkably active area of research over the past decade. Much of the scientific and technological work associated with microfluidic cell separation has been driven by needs in clinical diagnostics and therapeutic monitoring, most notably in the context of circulating tumor cells. The last several years have seen advances in a broad range of separation modalities ranging from miniaturized analogs of established techniques such as fluorescence- and magnetic-activated cell sorting (FACS and MACS, respectively), to more specialized approaches based on affinity, dielectrophoretic mobility, and inertial properties of cells. With several of these technologies nearing commercialization, there is a sense that the field of microfluidic cell separation has achieved a high level of maturity over an unusually short span of time. In this Perspective, we set the stage by describing major scientific and technological advances in this field and ask what the future holds. While many scientific questions remain unanswered and new compelling questions will undoubtedly arise, the relative maturity of this field poses some unique challenges.
Keywords	commercialization ; diagnostic techniques ; dielectrophoresis ; monitoring ; neoplasm cells
Language	English
Dates of publication	2014-1202
Size	p. 11481-11488.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021%2Fac5013283
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Soluble Receptor for Advanced Glycation End Products (sRAGE) Is a Sensitive Biomarker in Human Pulmonary Arterial Hypertension

Franziska Diekmann / Philippe Chouvarine / Hannes Sallmon / Louisa Meyer-Kobbe / Moritz Kieslich / Brian D. Plouffe / Shashi K. Murthy / Ralf Lichtinghagen / Ekaterina Legchenko / Georg Hansmann

International Journal of Molecular Sciences, Vol 22, Iss 8591, p

2021 Volume 8591

Abstract	Pulmonary arterial hypertension (PAH) is a progressive condition with an unmet need for early diagnosis, better monitoring, and risk stratification. The receptor for advanced glycation end products (RAGE) is activated in response to hypoxia and vascular injury, and is associated with inflammation, cell proliferation and migration in PAH. For the adult cohort, we recruited 120 patients with PAH, 83 with idiopathic PAH (IPAH) and 37 with connective tissue disease-associated PAH (CTD-PAH), and 48 controls, and determined potential plasma biomarkers by enzyme-linked immunoassay. The established heart failure marker NTproBNP and IL-6 plasma levels were several-fold higher in both adult IPAH and CTD-PAH patients versus controls. Plasma soluble RAGE (sRAGE) was elevated in IPAH patients (3044 ± 215.2 pg/mL) and was even higher in CTD-PAH patients (3332 ± 321.6 pg/mL) versus controls (1766 ± 121.9 pg/mL; p < 0.01). All three markers were increased in WHO functional class II+III PAH versus controls ( p < 0.001). Receiver-operating characteristic analysis revealed that sRAGE has diagnostic accuracy comparable to prognostic NTproBNP, and even outperforms NTproBNP in the distinction of PAH FC I from controls. Lung tissue RAGE expression was increased in IPAH versus controls (mRNA) and was located predominantly in the PA intima, media, and inflammatory cells in the perivascular space (immunohistochemistry). In the pediatric cohort, plasma sRAGE concentrations were higher than in adults, but were similar in PH (n = 10) and non-PH controls (n = 10). Taken together, in the largest adult sRAGE PAH study to date, we identify plasma sRAGE as a sensitive and accurate PAH biomarker with better performance than NTproBNP in the distinction of mild PAH from controls.
Keywords	soluble receptor for advanced glycation end products (sRAGE) ; pulmonary arterial hypertension ; biomarker ; vascular injury ; inflammation ; proliferation ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Mental health services use among Canadian Armed Forces members and Veterans: Data from the 2018 Canadian Armed Forces members and Veterans mental health follow-up survey (CAFMVHS).

St Cyr, Kate / Liu, Aihua / Plouffe, Rachel A / Nouri, Maede S / Forchuk, Callista A / Wanklyn, Sonya G / Bird, Brian M / Fikretoglu, Deniz / Mahar, Alyson L / Nazarov, Anthony / Richardson, J Don

Frontiers in health services

2022 Volume 2, Page(s) 954914

Abstract: Background: Differences in healthcare delivery systems and pathways to mental healthcare for Canadian Armed Forces (CAF) members and Veterans may contribute to variations in mental health services use (MHSU) and the factors associated with it. We: (1) ... ...

Abstract	Background: Differences in healthcare delivery systems and pathways to mental healthcare for Canadian Armed Forces (CAF) members and Veterans may contribute to variations in mental health services use (MHSU) and the factors associated with it. We: (1) estimated the prevalence of past 12-month MHSU (≥1 visit with a medical or mental health professional); and (2) identified sociodemographic, military-, trauma-, and health-related variables associated with MHSU among CAF members and Veterans. Methods: The current study used data from the 2018 CAF Members and Veterans Mental Health Follow-Up Survey (CAFVMHS). Model variables were selected a priori, and their respective associations with MHSU were estimated among (1) CAF members and (2) Veterans using separate multivariable logistic regression models. Results: Similar proportions of CAF members and Veterans reported past 12-month MHSU (26.9 vs. 27.5%, respectively). For both CAF members and Veterans, meeting criteria for at least one past 12-month MH disorder was associated with past 12-month MHSU [adjusted odds ratio (AOR) = 7.80, 95% confidence interval (CI) = 7.18-8.46; and AOR = 11.82, 95% CI: 11.07-12.61, respectively). Past-year suicide ideation, a history of sexual trauma, and endorsement of adverse childhood experiences were also significantly associated with MHSU among CAF members and Veterans. Significance: Similar to previous research, meeting screening criteria for a past 12-month MH disorder was strongly associated with MHSU among both samples. This study extends our existing knowledge about factors associated with MHSU among CAF members and Veterans, and offers direction for future research to increase MHSU.
Language	English
Publishing date	2022-08-22
Publishing country	Switzerland
Document type	Journal Article
ISSN	2813-0146
ISSN (online)	2813-0146
DOI	10.3389/frhs.2022.954914
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Article ; Online: Computational design optimization for microfluidic magnetophoresis.

Plouffe, Brian D / Lewis, Laura H / Murthy, Shashi K

Biomicrofluidics

2011 Volume 5, Page(s) 13413

Abstract: Current macro- and microfluidic approaches for the isolation of mammalian cells are limited in both efficiency and purity. In order to design a robust platform for the enumeration of a target cell population, high collection efficiencies are required. ... ...

Abstract	Current macro- and microfluidic approaches for the isolation of mammalian cells are limited in both efficiency and purity. In order to design a robust platform for the enumeration of a target cell population, high collection efficiencies are required. Additionally, the ability to isolate pure populations with minimal biological perturbation and efficient off-chip recovery will enable subcellular analyses of these cells for applications in personalized medicine. Here, a rational design approach for a simple and efficient device that isolates target cell populations via magnetic tagging is presented. In this work, two magnetophoretic microfluidic device designs are described, with optimized dimensions and operating conditions determined from a force balance equation that considers two dominant and opposing driving forces exerted on a magnetic-particle-tagged cell, namely, magnetic and viscous drag. Quantitative design criteria for an electromagnetic field displacement-based approach are presented, wherein target cells labeled with commercial magnetic microparticles flowing in a central sample stream are shifted laterally into a collection stream. Furthermore, the final device design is constrained to fit on standard rectangular glass coverslip (60 (L)×24 (W)×0.15 (H) mm(3)) to accommodate small sample volume and point-of-care design considerations. The anticipated performance of the device is examined via a parametric analysis of several key variables within the model. It is observed that minimal currents (<500 mA) are required to generate magnetic fields sufficient to separate cells from the sample streams flowing at rate as high as 7 ml∕h, comparable to the performance of current state-of-the-art magnet-activated cell sorting systems currently used in clinical settings. Experimental validation of the presented model illustrates that a device designed according to the derived rational optimization can effectively isolate (∼100%) a magnetic-particle-tagged cell population from a homogeneous suspension even in a low abundance. Overall, this design analysis provides a rational basis to select the operating conditions, including chamber and wire geometry, flow rates, and applied currents, for a magnetic-microfluidic cell separation device.
Language	English
Publishing date	2011-03-30
Publishing country	United States
Document type	Journal Article
ISSN	1932-1058
ISSN (online)	1932-1058
DOI	10.1063/1.3553239
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