Article ; Online: Immediate and Delayed (Late-Phase) Dermal Contact Sensitivity Reactions in Guinea Pigs
International Archives of Allergy and Immunology
1993 Volume 101, Issue 1, Page(s) 72–81
Abstract: Specific immediate and delayed (24-hour) local dermal reactivity to oxazolone and dinitrochlorobenzene (DNCB) was passively transferred to naive guinea pigs by the intradermal injection of immune guinea pig serum and its IgG1 fraction. In both actively ... ...
| Abstract | Specific immediate and delayed (24-hour) local dermal reactivity to oxazolone and dinitrochlorobenzene (DNCB) was passively transferred to naive guinea pigs by the intradermal injection of immune guinea pig serum and its IgG1 fraction. In both actively sensitized and passively sensitized animals, neutrophils were the major cells present in the immediate reaction to the specific contactant. Basophils and mononuclear cells were the major cells present in the delayed reaction to the contactant. This late-phase reaction is, therefore, a cutaneous basophil hypersensitivity (CBH) response. The serum factor that passively transferred this CBH response was stable when heated at 56°C for 1-4 h. Since transfer factor, cutaneous basophil hypersensitivity factor and guinea pig IgE are heat-sensitive, these factors probably made little or no contribution to this response. Because proteinase inhibitors are known to inhibit mast-cell degranulation in vitro, we tested the effect of soybean proteinase inhibitor in vivo. This inhibitor suppressed both the immediate and the delayed skin reactivities mediated by intradermal contactant-specific IgG1. These studies support the following concept: IgGl1in guinea pigs (and IgE in human beings) sensitize mast cells to specific antigens. Such antigens degranulate mast cells, releasing histamine and other mediators for the immediate hypersensitivity reaction, and cause mast cells to produce cytokines that recruit basophils, eosinophils and mononuclear cells for the late-phase (CBH) reaction. |
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| Keywords | Hypersensitivity, immediate ; Late-phase reactions ; Cutaneous basophil hypersensitivity ; Contact sensitivity ; Oxazolone ; Dinitrochlorobenzene ; Mast cells, guinea pigs ; IgG1 antibody ; Proteinase inhibitor | |||||
| Language | English | |||||
| Publisher | S. Karger AG | |||||
| Publishing place | Basel | |||||
| Publishing country | Switzerland | |||||
| Document type | Article ; Online | |||||
| ZDB-ID | 1108932-5 | |||||
| ISSN | 1423-0097 ; 1018-2438 ; 1018-2438 | |||||
| ISSN (online) | 1423-0097 | |||||
| ISSN | 1018-2438 | |||||
| DOI | 10.1159/000236501 | |||||
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| Database | Karger publisher's database |
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