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  1. Article ; Online: Double-stranded RNA mediated knockdown of sucrase gene induced mortality and reduced offspring production in Aphis gossypii.

    Uma, Gadigavarahalli Subbareddy / Saakre, Manjesh / Singh, Jyoti / Kalia, Vinay K

    Functional & integrative genomics

    2023  Volume 23, Issue 4, Page(s) 305

    Abstract: The importance of gut sucrase in maintaining osmotic equilibrium and utilizing phloem contents as a carbon source has been widely investigated and proven in sap-sucking insects. In the present study, silencing of Aphis gossypii sucrase1 (Agsuc1) was ... ...

    Abstract The importance of gut sucrase in maintaining osmotic equilibrium and utilizing phloem contents as a carbon source has been widely investigated and proven in sap-sucking insects. In the present study, silencing of Aphis gossypii sucrase1 (Agsuc1) was carried out by double-stranded RNA (dsRNA), which would be lethal to it due to disruption of osmotic balance. The dsRNA corresponding to Agsuc1 was synthesized by two methods, i.e., in vitro synthesis using T7/SP6 RNA polymerase and in vivo synthesis by bacterial expression, i.e., Escherichia coli strain HT115 transformed with the L4440 vector system. Oral delivery of double-stranded Agsuc1 synthesized in vitro (dsAgsuc1) and in vivo (HT115Agsuc1) induced around 50% mortality in nymphs of A. gossypii. Moreover, the number of offspring produced by the survived aphids decreased by 39-43%. Parthenogenetic reproduction of the aphids is the critical factor for their fast population build-up, leading to yield losses of economic significance. Thus, the present study demonstrated that the silencing of the Agsuc1 gene reduced the aphid population by killing it and inhibited the population buildup by reducing the number of offspring produced by the survived aphids, likely to result in a significant reduction in crop damage. The production of dsRNA by bacterial expression is a cost-effective method. It has the potential to be used as a biopesticide. The sucrase gene is an excellent putative target gene for RNAi against A. gossypii. It could be used to develop a transgenic plant that produces dsAgsuc1 to keep A. gossypii populations below the economic threshold level.
    MeSH term(s) Animals ; RNA, Double-Stranded/genetics ; Aphids/genetics ; Biological Control Agents
    Chemical Substances RNA, Double-Stranded ; Biological Control Agents
    Language English
    Publishing date 2023-09-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2014670-X
    ISSN 1438-7948 ; 1438-793X
    ISSN (online) 1438-7948
    ISSN 1438-793X
    DOI 10.1007/s10142-023-01233-7
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  2. Article ; Online: Perspectives on plant flavonoid quercetin-based drugs for novel SARS-CoV-2.

    Saakre, Manjesh / Mathew, Deepu / Ravisankar, V

    Beni-Suef University journal of basic and applied sciences

    2021  Volume 10, Issue 1, Page(s) 21

    Language English
    Publishing date 2021-03-24
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2768279-1
    ISSN 2314-8543 ; 2314-8535 ; 2314-8543
    ISSN (online) 2314-8543
    ISSN 2314-8535 ; 2314-8543
    DOI 10.1186/s43088-021-00107-w
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  3. Article ; Online: Perspectives on plant flavonoid quercetin-based drugs for novel SARS-CoV-2

    Manjesh Saakre / Deepu Mathew / V. Ravisankar

    Beni-Suef University Journal of Basic and Applied Sciences, Vol 10, Iss 1, Pp 1-

    2021  Volume 13

    Abstract: Abstract Background The world pandemic COVID-19 caused by SARS-CoV-2 is currently claiming thousands of lives. Flavonoids abundantly present in the fruits and vegetables, especially quercetin, are shown to have antiviral activities. Main text This paper ... ...

    Abstract Abstract Background The world pandemic COVID-19 caused by SARS-CoV-2 is currently claiming thousands of lives. Flavonoids abundantly present in the fruits and vegetables, especially quercetin, are shown to have antiviral activities. Main text This paper reviews the capability of the plant flavonoid quercetin to fight the novel coronavirus and the possibility for drug development based on this. The mode of action explaining the known pathways through which this molecule succeeds in the antiviral activity, action of quercetin on SARS-CoV-2 main protease 3CLpro, antiviral activities of its derivatives on human viruses, effect of combination of zinc co-factor along with quercetin in the COVID-19 treatment, and the regulation of miRNA genes involved in the viral pathogenesis are discussed. Proof for this concept is provided following the virtual screening using ten key enzymes of SARS-CoV-2 and assessing their interactions. Active residues in the 3D structures have been predicted using CASTp and were docked against quercetin. Key proteins 3CLpro, spike glycoprotein/ human ACE2-BOAT1 complex, RNA-dependent RNA polymerase, main peptidase, spike glycoprotein, RNA replicase, RNA binding protein, papain-like protease, SARS papain-like protease/ deubiquitinase, and complex of main peptidase with an additional Ala at the N-terminus of each protomer, have shown the binding energies ranging between − 6.71 and − 3.37 kcal/ Mol, showing that quercetin is a potential drug candidate inhibiting multiple SARS-CoV-2 enzymes. Conclusion The antiviral properties of flavonoid and the molecular mechanisms involved are reviewed. Further, proof for this concept is given by docking of key proteins from SARS-CoV-2 with quercetin. Graphical abstract
    Keywords COVID-19 ; Drug design ; Antiviral ; Phytomedicine ; 3CLpro ; Zinc ; Medicine (General) ; R5-920 ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
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  4. Article ; Online: Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.

    Saakre, Manjesh / Jaiswal, Sandeep / Rathinam, Maniraj / Raman, K Venkat / Tilgam, Jyotsana / Paul, Krishnayan / Sreevathsa, Rohini / Pattanayak, Debasis

    Molecular biotechnology

    2023  

    Abstract: Insect-pests infestation greatly affects global agricultural production and is projected to become more severe in upcoming years. There is concern about pesticide application being ineffective due to insect resistance and environmental toxicity. Reduced ... ...

    Abstract Insect-pests infestation greatly affects global agricultural production and is projected to become more severe in upcoming years. There is concern about pesticide application being ineffective due to insect resistance and environmental toxicity. Reduced effectiveness of Bt toxins also made the scientific community shift toward alternative strategies to control devastating agricultural pests. With the advent of host-delivered RNA interference, also known as host-induced gene silencing, targeted insect genes have been suppressed through genetic engineering tools to deliver a novel insect-pest resistance strategy for combating a number of agricultural pests. This review recapitulates the possible mechanism of host-delivered RNA interference (HD-RNAi), in particular, the silencing of target genes of insect-pests. We emphasize the development of the latest strategies against evolving insect targets including designing of artificial microRNAs, vector constructs, and the benefit of using plastid transformation to transform target RNA-interfering genes. Advantages of using HD-RNAi over other small RNA delivery modes and also the supremacy of HD-RNAi over the CRISPR-Cas system particularly for insect resistance have been described. However, the broader application of this technology is restricted due to its several limitations. Using artificial miRNA designs, the host-delivered RNAi + Bt combinatorial approach and chloroplast transformation can overcome limitations of RNAi. With careful design and delivery approaches, RNAi promises to be extremely valuable and effective plant protection strategy to attain durable insect-pest resistance in crops. Development of transgenic plant using novel strategies to achieve durable resistance against the target insect.
    Language English
    Publishing date 2023-07-31
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1193057-3
    ISSN 1559-0305 ; 1073-6085
    ISSN (online) 1559-0305
    ISSN 1073-6085
    DOI 10.1007/s12033-023-00833-9
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  5. Article: Amelioration of cold-induced sweetening in potato by RNAi mediated silencing of

    Jaiswal, Sandeep / Paul, Krishnayan / Raman, K Venkat / Tyagi, Saurabh / Saakre, Manjesh / Tilgam, Jyotsana / Bhattacharjee, Sougata / Vijayan, Joshitha / Mondal, Kalyan Kumar / Sreevathsa, Rohini / Pattanayak, Debasis

    Frontiers in plant science

    2023  Volume 14, Page(s) 1133029

    Abstract: Cold-induced sweetening (CIS) is an unwanted physiological phenomenon in which reducing sugars (RS) get accumulated in potato ( ...

    Abstract Cold-induced sweetening (CIS) is an unwanted physiological phenomenon in which reducing sugars (RS) get accumulated in potato (
    Language English
    Publishing date 2023-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1133029
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  6. Article ; Online: Genome Editing for Resistance to Insect Pests

    Shaily Tyagi / Karthik Kesiraju / Manjesh Saakre / Maniraj Rathinam / Venkat Raman / Debasis Pattanayak / Rohini Sreevathsa

    ACS Omega, Vol 5, Iss 33, Pp 20674-

    An Emerging Tool for Crop Improvement

    2020  Volume 20683

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2020-08-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
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  7. Article ; Online: Identification, characterization, and comprehensive expression profiling of floral master regulators in pigeon pea (Cajanus cajan [L.] Millspaugh).

    Bhattacharjee, Sougata / Bhowmick, Rakesh / Paul, Krishnayan / Venkat Raman, K / Jaiswal, Sandeep / Tilgam, Jyotsana / Saakre, Manjesh / Kumari, Priyanka / Baaniya, Mahi / Vijayan, Joshitha / Sreevathsa, Rohini / Pattanayak, Debasis

    Functional & integrative genomics

    2023  Volume 23, Issue 4, Page(s) 311

    Abstract: Pigeon pea is an important protein-rich pulse crop. Identification of flowering master regulators in pigeon pea is highly imperative as indeterminacy and late flowering are impediments towards yield improvement. A genome-wide analysis was performed to ... ...

    Abstract Pigeon pea is an important protein-rich pulse crop. Identification of flowering master regulators in pigeon pea is highly imperative as indeterminacy and late flowering are impediments towards yield improvement. A genome-wide analysis was performed to explore flowering orthologous groups in pigeon pea. Among the 412 floral orthologs identified in pigeon pea, 148 genes belong to the meristem identity, photoperiod-responsive, and circadian clock-associated ortholog groups. Our comparative genomics study revealed purifying selection pressures (ka/ks) on floral orthologs, and duplication patterns and evolution through synteny with other model species. Phylogenetic analysis of floral genes substantiated a connection between pigeon pea plant architecture and flowering time as all the PEBP domain-containing genes belong to meristem identity floral networks of pigeon pea. Expression profiling of eleven major orthologs in contrasting determinate and indeterminate genotypes indicated that these orthologs might be involved in flowering regulation. Expression of floral inducer, FT, and floral repressor, TFL1, was non-comparable in indeterminate genotypes across all the developmental stages of pigeon pea. However, dynamic FT/TFL1 expression ratio detected in all tissues of both the genotypes suggested their role in floral transition. One TFL1 ortholog having high sequence conserveness across pigeon pea genotypes showed differential expression indicating genotype-dependent regulation of this ortholog. Presence of conserved 6mA-methylation patterns in light-responsive elements and in other cis-regulatory elements of FT and TFL1 across different plant genotypes indicated possible involvement of epigenetic regulation in flowering.
    MeSH term(s) Cajanus/genetics ; Epigenesis, Genetic ; Phylogeny ; Genotype ; Genomics
    Language English
    Publishing date 2023-09-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2014670-X
    ISSN 1438-7948 ; 1438-793X
    ISSN (online) 1438-7948
    ISSN 1438-793X
    DOI 10.1007/s10142-023-01236-4
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  8. Article ; Online: Genome Editing for Resistance to Insect Pests: An Emerging Tool for Crop Improvement.

    Tyagi, Shaily / Kesiraju, Karthik / Saakre, Manjesh / Rathinam, Maniraj / Raman, Venkat / Pattanayak, Debasis / Sreevathsa, Rohini

    ACS omega

    2020  Volume 5, Issue 33, Page(s) 20674–20683

    Abstract: Plants are challenged incessantly by several biotic and abiotic stresses during their entire growth period. As with other biotic stress factors, insect pests have also posed serious concerns related to yield losses due to which agricultural productivity ... ...

    Abstract Plants are challenged incessantly by several biotic and abiotic stresses during their entire growth period. As with other biotic stress factors, insect pests have also posed serious concerns related to yield losses due to which agricultural productivity is at stake. In plants, trait modification for crop improvement was initiated with breeding approaches followed by genetic engineering. However, stringent regulatory policies for risk assessment and lack of social acceptance for genetically modified crops worldwide have incited researchers toward alternate strategies. Genome engineering or genome editing has emerged as a new breeding technique with the ability to edit the genomes of plants, animals, microbes, and human beings. Several gene editing strategies are being executed with continuous emergence of variants. The scientific community has unraveled the utility of various editing tools from endonucleases to CRISPR/Cas in several aspects related to plant growth, development, and mitigation of stresses. The categorical focus on the development of tools and techniques including designing of binary vectors to facilitate ease in genome engineering are being pursued. Through this Review, we embark upon the conglomeration of various genome editing strategies that can be and are being used to design insect pest resistance in plants. Case studies and novel crop-based approaches that reiterate the successful use of these tools in insects as well as in plants are highlighted. Further, the Review also provides implications for the requirement of a specific regulatory framework and risk assessment of the edited crops. Genome editing toward insect pest management is here to stay, provided uncompromising efforts are made toward the identification of amiable target genes.
    Language English
    Publishing date 2020-08-11
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.0c01435
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens

    Saakre, Manjesh / Abida Puthenpeedikal Salim / George Thomas / Rose Mary Ffancies / Sajeevan Radha Sivarajan / Thirthikar Meera Baburao / Valasala Poothecty Achuthan

    China National Rice Research Institute Rice science. 2017 Sept., v. 24, no. 5

    2017  

    Abstract: Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential. Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants ... ...

    Abstract Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential. Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress. In the present study, differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water, Pseudomonas fluorescens strain (Pf1) treated plants subjected for drought stress by withholding water and control (well-watered). Differentially expressed cDNAs of six genes (COX1, PKDP, bZIP1, AP2-EREBP, Hsp20 and COC1) were identified, cloned and sequenced. Real-time qPCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1. This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage. These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization, which might be a key element for induced systemic tolerance by microbes.
    Keywords complementary DNA ; crops ; drought ; drought tolerance ; gene expression regulation ; genes ; microorganisms ; molecular cloning ; Pseudomonas fluorescens ; quantitative polymerase chain reaction ; rice ; sequence analysis ; water stress
    Language English
    Dates of publication 2017-09
    Size p. 291-298.
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 1672-6308
    DOI 10.1016/j.rsci.2017.04.005
    Database NAL-Catalogue (AGRICOLA)

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