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Article: Riboflavin compounds show NAD(P)H dependent quinone oxidoreductase-like quinone reducing activity.

Nagase, Midori / Sakamoto, Miku / Amekura, Sakiko / Akiba, Sayaka / Kashiba, Misato / Yokoyama, Kenji / Yamamoto, Yorihiro / Fujisawa, Akio

Journal of clinical biochemistry and nutrition

2023 Volume 73, Issue 1, Page(s) 52–60

Abstract: NAD(P)H-dependent quinone oxidoreductase (NQO) is an essential enzyme in living organisms and cells ... protecting them from oxidative stress. NQO reduces coenzyme Q (CoQ) using NAD(P)H as an electron donor ...

Abstract	NAD(P)H-dependent quinone oxidoreductase (NQO) is an essential enzyme in living organisms and cells protecting them from oxidative stress. NQO reduces coenzyme Q (CoQ) using NAD(P)H as an electron donor. In the present study, we searched for coenzyme Q10 reducing activity from fractions of gel filtration-fractionated rat liver homogenate. In addition to the large-molecular-weight fraction containing NQO, CoQ10 reducing activity was also detected in a low-molecular-weight fraction. Furthermore, dicumarol, a conventional inhibitor of NQO1 (DT diaphorase), did not inhibit the reduction but quercetin did, suggesting that the activity was not due to NQO1. After further purification, the NADH-dependent CoQ10-reducing compound was identified as riboflavin. Riboflavin is an active substituent of other flavin compounds such as FAD and FMN. These flavin compounds also reduced not only CoQ homologues but also vitamin K homologues in the presence of NADH. The mechanism was speculated to work as follows: NADH reduces flavin compounds to the corresponding reduced forms, and subsequently, the reduced flavin compounds immediately reduce bio-quinones. Furthermore, the flavin-NADH system reduces CoQ10 bound with saposin B, which is believed to function as a CoQ transfer protein
Language	English
Publishing date	2023-05-16
Publishing country	Japan
Document type	Journal Article
ZDB-ID	632945-7
ISSN	1880-5086 ; 0912-0009
ISSN (online)	1880-5086
ISSN	0912-0009
DOI	10.3164/jcbn.22-140
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Article ; Online: Functional importance of the oligomer formation of the cyanobacterial H

Iizuka, Azusa / Kajimoto, Kousuke / Fujisawa, Tomotsumi / Tsukamoto, Takashi / Aizawa, Tomoyasu / Kamo, Naoki / Jung, Kwang-Hwan / Unno, Masashi / Demura, Makoto / Kikukawa, Takashi

Scientific reports

2019 Volume 9, Issue 1, Page(s) 10711

Abstract: ... not been well clarified. We examined the effects of oligomerization of a H ...

Abstract	Many microbial rhodopsins self-oligomerize, but the functional consequences of oligomerization have not been well clarified. We examined the effects of oligomerization of a H
MeSH term(s)	Amino Acid Motifs ; Amino Acid Substitution ; Aspartic Acid/chemistry ; Aspartic Acid/genetics ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cyanobacteria/metabolism ; Histidine/chemistry ; Histidine/genetics ; Protein Multimerization ; Protons ; Rhodopsin/chemistry ; Rhodopsin/genetics ; Rhodopsin/metabolism
Chemical Substances	Bacterial Proteins ; Protons ; Aspartic Acid (30KYC7MIAI) ; Histidine (4QD397987E) ; Rhodopsin (9009-81-8)
Language	English
Publishing date	2019-07-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-47178-5
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Article ; Online: The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function.

Fujisawa, Madoka / Yasumoto, Atsushi / Kato, Hideki / Sugawara, Yuka / Yoshida, Yoko / Yatomi, Yutaka / Nangaku, Masaomi

British journal of haematology

2019 Volume 189, Issue 1, Page(s) 182–186

Abstract: ... such as production of complement factor H (CFH) autoantibodies. The growing evidence indicates complement ...

Abstract	Atypical haemolytic uremic syndrome (aHUS) is associated with complement system abnormality, such as production of complement factor H (CFH) autoantibodies. The growing evidence indicates complement overactivation on platelets is intimately involved in aHUS pathogenesis, besides endothelial injury. We here showed plasma from patients with anti-CFH antibodies induced aggregation of washed platelets, while purified anti-CFH antibodies suppressed aggregation. This suggested anti-CFH antibody itself suppressed thrombosis, while other plasma factor including complement factors could overactivate the platelets, leading to aggregation, which augmented the notion the state of complement activation influenced by anti-CFH antibodies is important in the aggregation of platelets in aHUS.
MeSH term(s)	Atypical Hemolytic Uremic Syndrome/blood ; Atypical Hemolytic Uremic Syndrome/immunology ; Atypical Hemolytic Uremic Syndrome/pathology ; Autoantibodies/blood ; Autoantibodies/immunology ; Blood Platelets/immunology ; Blood Platelets/metabolism ; Blood Platelets/pathology ; Child ; Child, Preschool ; Complement Factor H/immunology ; Female ; Humans ; Male ; Platelet Aggregation/immunology
Chemical Substances	Autoantibodies ; Complement Factor H (80295-65-4)
Language	English
Publishing date	2019-12-26
Publishing country	England
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.16297
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Article ; Online: Development of MHFA-based 2-h educational program for early intervention in depression among office workers: A single-arm pilot trial.

Kubo, Hiroaki / Urata, Hiromi / Katsuki, Ryoko / Hirashima, Miyako / Ueno, Shion / Suzuki, Yuriko / Fujisawa, Daisuke / Hashimoto, Naoki / Kobara, Keiji / Cho, Tetsuji / Mitsui, Toshiko / Kanba, Shigenobu / Otsuka, Kotaro / Kato, Takahiro A

PloS one

2018 Volume 13, Issue 12, Page(s) e0208114

Abstract: ... trial.: Methods: The program is a two-hour (2-h) training course for employees based on the Mental Health ...

Abstract	Objective: In the workplace depression and suicide are serious mental health problems. A lack of knowledge and mental health skills along with the stigma toward mental health problems often results in delays in seeking professional help. Interventions targeting not only persons with mental health problems but also people around the individual are warranted in order to encourage supporting behavior within entire workplace. In the present study, we investigated the efficacy of our newly developed educational training program in the management with depression and suicidal risk in the workplace as a single-arm pilot trial. Methods: The program is a two-hour (2-h) training course for employees based on the Mental Health First Aid (MHFA) program which aims to increase public mental health literacy. We conducted this program at a company workplace among 91 employees, and ultimately 83 participants completed the self-rated questionnaires. Changes in confidence and practical skills in early intervention of depression and suicide-prevention, and stigma toward mental health problems were evaluated using self-rated questionnaires at 3 time-points; pre-program, immediately post-program, and 1 month after the program. Results: Confidence and practical skills were significantly improved even 1 month after the program, and stigma reduced just after the program. Conclusions: Our pilot study suggests that the program has a positive impact on encouraging employees to support their co-workers with mental health problems, and is applicable for busy workers due to its short duration. A single-arm design, evaluation using self-rated questionnaire and short-term follow up period are the main limitations of the present study. Hence, future research is required to validate the effects of this program with control groups, and also to assess long-term effectiveness and objective changes such as absenteeism and sick leave. Trial registration: UMIN Clinical Trials Registry (UMIN-CTR) R000023258.
MeSH term(s)	Adult ; Depression/prevention & control ; Depression/psychology ; Female ; Health Knowledge, Attitudes, Practice ; Health Literacy/organization & administration ; Humans ; Male ; Mental Health/education ; Occupational Stress/psychology ; Office Management ; Pilot Projects ; Program Evaluation ; Social Stigma ; Suicide/prevention & control ; Suicide/psychology ; Workplace/organization & administration ; Young Adult
Language	English
Publishing date	2018-12-07
Publishing country	United States
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0208114
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Article ; Online: Photoinduced H-aggregation of cationic dyes on metal-oxide surfaces via light activated molecular migration.

Fujisawa, Jun-Ichi / Murai, Karin / Hanaya, Minoru

Chemical communications (Cambridge, England)

2019 Volume 55, Issue 97, Page(s) 14641–14644

Abstract: ... we report visible light induced H-aggregation of cationic dyes (methylene blue and toluidine blue ...

Abstract	Molecular transport by use of light energy is a new photofunction for light powered molecular carriers and light induced nano-structure formation on a substrate. However, this research field has not been cultivated yet. In order to understand the basic mechanism and potentialities, experimental observations of photoactivated molecular migration and its functions in various systems are required. Here, we report visible light induced H-aggregation of cationic dyes (methylene blue and toluidine blue) on metal-oxide surfaces (TiO
Language	English
Publishing date	2019-11-20
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c9cc05951b
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Article ; Online: Gait characterization for osteoarthritis patients using wearable gait sensors (H-Gait systems).

Tadano, Shigeru / Takeda, Ryo / Sasaki, Keita / Fujisawa, Tadashi / Tohyama, Harukazu

Journal of biomechanics

2016 Volume 49, Issue 5, Page(s) 684–690

Abstract: ... H-Gait system in an actual clinical trial and proposes new gait parameters for characterizing OA ... gait. Seven H-Gait sensors, consisting of tri-axial inertial sensors, were attached to seven lower limb ...

Abstract	The objective of this work was to investigate the possibilities of using the wearable sensors-based H-Gait system in an actual clinical trial and proposes new gait parameters for characterizing OA gait. Seven H-Gait sensors, consisting of tri-axial inertial sensors, were attached to seven lower limb body segments (pelvis, both thighs, both shanks and both feet). The acceleration and angular velocity data measured were used to estimate three-dimensional kinematic parameters of patients during level walking. Three new parameters were proposed to assess the severity of OA based on the characteristics of these joint center trajectories in addition to conventional gait spatio-temporal parameters. The experiment was conducted on ten subjects with knee OA. The kinematic results obtained (hip, knee and ankle joint angles, joint trajectory in the horizontal and sagittal planes) were compared with those from a reference healthy (control) group. As a result, the angle between the right and left knee trajectories along with that of the ankle joint trajectories were almost twice as large (21.3° vs. 11.6° and 14.9° vs. 7.8°) compared to those of the healthy subjects. In conclusion, it was found that the ankle joints during stance abduct less to avoid adduction at the knee as the severity of OA increases and lead to more acute angles (less parallel) between the right and left knee/ankle joints in the horizontal plane. This method was capable to provide quantitative information about the gait of OA patients and has the advantage to allow for out-of-laboratory monitoring.
MeSH term(s)	Acceleration ; Aged ; Biomechanical Phenomena ; Gait ; Humans ; Monitoring, Physiologic/instrumentation ; Osteoarthritis/physiopathology ; Young Adult
Language	English
Publishing date	2016-03-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	218076-5
ISSN	1873-2380 ; 0021-9290
ISSN (online)	1873-2380
ISSN	0021-9290
DOI	10.1016/j.jbiomech.2016.01.017
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Article ; Online: Development of a 2-h suicide prevention program for medical staff including nurses and medical residents: A two-center pilot trial.

Journal of affective disorders

2018 Volume 225, Page(s) 569–576

Abstract: ... Methods: We developed a 2-h suicide intervention program for medical staff, based on the Mental Health ...

Abstract	Background: Suicide is a crucial global health concern and effective suicide prevention has long been warranted. Mental illness, especially depression is the highest risk factor of suicide. Suicidal risk is increased in people not only with mental illness but also with physical illnesses, thus medical staff caring for physically-ill patients are also required to manage people with suicidal risk. In the present study, we evaluated our newly developed suicide intervention program among medical staff. Methods: We developed a 2-h suicide intervention program for medical staff, based on the Mental Health First Aid (MHFA), which had originally been developed for the general population. We conducted this program for 74 medical staff members from 2 hospitals. Changes in knowledge, perceived skills, and confidence in early intervention of depression and suicide-prevention were evaluated using self-reported questionnaires at 3 points; pre-program, immediately after the program, and 1 month after program. Results: This suicide prevention program had significant effects on improving perceived skills and confidence especially among nurses and medical residents. These significant effects lasted even 1 month after the program. Limitations: Design was a single-arm study with relatively small sample size and short-term follow up. Conclusions: The present study suggests that the major target of this effective program is nurses and medical residents. Future research is required to validate the effects of the program with control groups, and also to assess long-term effectiveness and actual reduction in suicide rates.
Language	English
Publishing date	2018-01-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	135449-8
ISSN	1573-2517 ; 0165-0327
ISSN (online)	1573-2517
ISSN	0165-0327
DOI	10.1016/j.jad.2017.08.074
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Article ; Online: Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin

Azusa Iizuka / Kousuke Kajimoto / Tomotsumi Fujisawa / Takashi Tsukamoto / Tomoyasu Aizawa / Naoki Kamo / Kwang-Hwan Jung / Masashi Unno / Makoto Demura / Takashi Kikukawa

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 12

Abstract: ... of oligomerization have not been well clarified. We examined the effects of oligomerization of a H+ pump, Gloeobacter ... showed faint M intermediate formation and negligible H+ transfer reactions. These changes reflected ... Here, we focused on His87, which is a neighboring residue of Asp121 and conserved among eubacterial H+ ...

Abstract	Abstract Many microbial rhodopsins self-oligomerize, but the functional consequences of oligomerization have not been well clarified. We examined the effects of oligomerization of a H+ pump, Gloeobacter rhodopsin (GR), by using nanodisc containing trimeric and monomeric GR. The monomerization did not appear to affect the unphotolyzed GR. However, we found a significant impact on the photoreaction: The monomeric GR showed faint M intermediate formation and negligible H+ transfer reactions. These changes reflected the elevated pKa of the Asp121 residue, whose deprotonation is a prerequisite for the functional photoreaction. Here, we focused on His87, which is a neighboring residue of Asp121 and conserved among eubacterial H+ pumps but replaced by Met in an archaeal H+ pump. We found that the H87M mutation removes the “monomerization effects”: Even in the monomeric state, H87M contained the deprotonated Asp121 and showed both M formation and distinct H+ transfer reactions. Thus, for wild-type GR, monomerization probably strengthens the Asp121-His87 interaction and thereby elevates the pKa of Asp121 residue. This strong interaction might occur due to the loosened protein structure and/or the disruption of the interprotomer interaction of His87. Thus, the trimeric assembly of GR enables light-induced H+ transfer reactions through adjusting the positions of key residues.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-07-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Ru(ii)-Pheox-catalyzed Si-H insertion reaction: construction of enantioenriched carbon and silicon centers.

Nakagawa, Yoko / Chanthamath, Soda / Fujisawa, Ikuhide / Shibatomi, Kazutaka / Iwasa, Seiji

Chemical communications (Cambridge, England)

2017 Volume 53, Issue 26, Page(s) 3753–3756

Abstract: We established a highly enantioselective Si-H insertion reaction to construct chiral centers ... at the carbon and silicon atoms, using a Ru(ii)-pheox catalyst. The catalytic asymmetric Si-H insertion reaction ...

Abstract	We established a highly enantioselective Si-H insertion reaction to construct chiral centers at the carbon and silicon atoms, using a Ru(ii)-pheox catalyst. The catalytic asymmetric Si-H insertion reaction of α-methyl-α-diazoesters proceeded smoothly with excellent stereoinduction at both the neighboring carbon and silicon atoms (up to 99% yield and 99% ee).
Language	English
Publishing date	2017-03-28
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c7cc01070b
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Article: Photoinduced H-aggregation of cationic dyes on metal-oxide surfaces via light activated molecular migration

Fujisawa, Jun-ichi / Murai, Karin / Hanaya, Minoru

Chemical communications. 2019 Dec. 3, v. 55, no. 97

2019

Abstract: ... we report visible light induced H-aggregation of cationic dyes (methylene blue and toluidine blue ...

Abstract	Molecular transport by use of light energy is a new photofunction for light powered molecular carriers and light induced nano-structure formation on a substrate. However, this research field has not been cultivated yet. In order to understand the basic mechanism and potentialities, experimental observations of photoactivated molecular migration and its functions in various systems are required. Here, we report visible light induced H-aggregation of cationic dyes (methylene blue and toluidine blue) on metal-oxide surfaces (TiO₂ and ZnO) via photoactivated surface migration of these dyes. Our research widely expands the scope of photoinduced surface molecular migration to the conventional dyes and metal-oxide surfaces, and also demonstrates that the photoinduced molecular assembly provides a photochromic function. It is noteworthy that high aggregates, which are difficult to form in the dark, can be formed by the photoinduced surface migration of excited dyes.
Keywords	chemical reactions ; energy ; light ; methylene blue ; nanomaterials ; titanium dioxide ; toluidine blue ; zinc oxide
Language	English
Dates of publication	2019-1203
Size	p. 14641-14644.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c9cc05951b
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