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  1. Article: Screening technologies for cervical cancer: Overview.

    Banerjee, Dipanwita / Mittal, Srabani / Mandal, Ranajit / Basu, Partha

    CytoJournal

    2022  Volume 19, Page(s) 23

    Abstract: Ever since the introduction of the Papanicolaou (PAP) smear test was published in 1941 in American Journal of Obstetrics and Gynecology, PAP test linked with definitive treatment has prevented millions of women from cervical cancer in the developed ... ...

    Abstract Ever since the introduction of the Papanicolaou (PAP) smear test was published in 1941 in American Journal of Obstetrics and Gynecology, PAP test linked with definitive treatment has prevented millions of women from cervical cancer in the developed countries. Due to limited availability of resources, a lack of infrastructure and difficulty in getting highly trained professionals, widespread implementation of PAP test dependent cervical cancer screening program has not been established in low and middle income countries such as India. Therefore, after availability of non-cytological tests such as visual inspection on acetic acid (VIA) and human papillomavirus (HPV) DNA test, there is a paradigm shift in cervical cancer screening methods. In past two decades, various research work has convincingly established the utility of VIA and HPV test in developing countries. The evidences were evaluated by the World Health Organization (WHO) and recommendations have been recently published for comprehensive cervical cancer control strategies for the low and middle income countries. For any successful screening program, achieving high coverage (>70%) of the target population rather than frequent screening is the most important determinant. It is also equally important to ensure appropriate investigations of the screen positive women to establish the disease and treatment of the screen detected cases of cervical intra epithelial neoplasia (CIN) and cancer. HPV testing is the WHO recommended test for cervical cancer screening especially in view of widespread HPV vaccination in young population leading to lower prevalence of CIN and other HPV related diseases.
    Language English
    Publishing date 2022-03-29
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2158838-7
    ISSN 1742-6413 ; 0974-5963
    ISSN (online) 1742-6413
    ISSN 0974-5963
    DOI 10.25259/CMAS_03_04_2021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Post-procedure adhesive arachnoiditis following obstetric spinal anaesthesia.

    Chattopadhyay, Ipsita / Jha, Amarendra Kumar / Banerjee, Sumantra Sarathi / Basu, Srabani

    Indian journal of anaesthesia

    2016  Volume 60, Issue 5, Page(s) 372–374

    Language English
    Publishing date 2016-05-19
    Publishing country India
    Document type Journal Article
    ZDB-ID 412570-8
    ISSN 0019-5049
    ISSN 0019-5049
    DOI 10.4103/0019-5049.181619
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article ; Online: Fluorescent multiplexing of 3D spheroids: Analysis of biomarkers using automated immunohistochemistry staining platform and multispectral imaging.

    Bhaumik, Srabani / Boyer, Jean / Banerjee, Chaitali / Clark, Samantha / Sebastiao, Noemi / Vela, Elizabeth / Towne, Penny

    Journal of cellular biochemistry

    2020  Volume 121, Issue 12, Page(s) 4974–4990

    Abstract: In preclinical cancer studies, three-dimensional (3D) cell spheroids and aggregates are preferred over monolayer cell cultures due to their architectural and functional similarity to solid tumors. We performed a proof-of-concept study to generate ... ...

    Abstract In preclinical cancer studies, three-dimensional (3D) cell spheroids and aggregates are preferred over monolayer cell cultures due to their architectural and functional similarity to solid tumors. We performed a proof-of-concept study to generate physiologically relevant and predictive preclinical models using non-small cell lung adenocarcinoma, and colon and colorectal adenocarcinoma cell line-derived 3D spheroids and aggregates. Distinct panels were designed to determine the expression profiles of frequently studied biomarkers of the two cancer subtypes. The lung adenocarcinoma panel included ALK, EGFR, TTF-1, and CK7 biomarkers, and the colon and colorectal adenocarcinoma panel included BRAF V600E, MSH2, MSH6, and CK20. Recent advances in immunofluorescence (IF) multiplexing and imaging technology enable simultaneous detection and quantification of multiple biomarkers on a single slide. In this study, we performed IF staining of multiple biomarkers per section on formalin-fixed paraffin-embedded 3D spheroids and aggregates. We optimized protocol parameters for automated IF and demonstrated staining concordance with automated chromogenic immunohistochemistry performed with validated protocols. Next, post-acquisition spectral unmixing of the captured fluorescent signals were utilized to delineate four differently stained biomarkers within a single multiplex IF image, followed by automated quantification of the expressed markers. This workflow has the potential to be adapted to preclinical high-throughput screening and drug efficacy studies utilizing 3D spheroids from cancer cell lines and patient-derived organoids. The process allows for cost, time, and resource savings through concurrent staining of several biomarkers on a single slide, the ability to study the interactions of multiple expressed proteins within a single region of interest, and enable quantitative assessment of biomarkers in cancer cells.
    Language English
    Publishing date 2020-07-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392402-6
    ISSN 1097-4644 ; 0730-2312
    ISSN (online) 1097-4644
    ISSN 0730-2312
    DOI 10.1002/jcb.29827
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice.

    Kar, Srabani / Mitra, Shinjini / Banerjee, Ena Ray

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1516, Page(s) 145–152

    Abstract: Stem cells are cells capable of proliferation, self-renewal, and differentiation into specific phenotypes. They are an essential part of tissue engineering, which is used in regenerative medicine in case of degenerative diseases. In this chapter, we ... ...

    Abstract Stem cells are cells capable of proliferation, self-renewal, and differentiation into specific phenotypes. They are an essential part of tissue engineering, which is used in regenerative medicine in case of degenerative diseases. In this chapter, we describe the methods of isolating and culturing various types of stem cells, like human embryonic stem cells (hESCs), human umbilical cord derived mesenchymal stem cells (hUC-MSCs), murine bone marrow derived mesenchymal stem cells (mBM-MSCs), murine adipose tissue derived mesenchymal stem cells (mAD-MSCs), and murine bone marrow derived dendritic cells (mBMDCs). All these cell types can be used in tissue engineering techniques.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/7651_2015_315
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Post-procedure adhesive arachnoiditis following obstetric spinal anaesthesia

    Ipsita Chattopadhyay / Amarendra Kumar Jha / Sumantra Sarathi Banerjee / Srabani Basu

    Indian Journal of Anaesthesia, Vol 60, Iss 5, Pp 372-

    2016  Volume 374

    Keywords Anesthesiology ; RD78.3-87.3 ; Surgery ; RD1-811 ; Medicine ; R
    Language English
    Publishing date 2016-01-01T00:00:00Z
    Publisher Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Effect of Sodium Cholate on the Phase Transition Temperature of Dipalmitoyl Phosphatidylcholine

    Banerjee, Srabani / S. N. Chatterjee

    Zeitschrift für Naturforschung C. 2014 June 2, v. 38, no. 3-4

    2014  

    Abstract: On treatment with sodium cholate, the phase transition temperature (Tc) of multilamellar liposomes derived from dipalmitoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P molar ratio. A molar ratio of 4.5 caused a one ...

    Abstract On treatment with sodium cholate, the phase transition temperature (Tc) of multilamellar liposomes derived from dipalmitoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P molar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles of DPPC exhibited the same Tc as that of the multilamellar ones. The formation of unilamellar vesicles from the multilam ellar ones by cholate treatm ent was investigated by electron microscopy
    Keywords electron microscopy ; phase transition ; phosphatidylcholines ; sodium ; temperature
    Language English
    Dates of publication 2014-0602
    Size p. 302-306.
    Publishing place Verlag der Zeitschrift für Naturforschung
    Document type Article
    ZDB-ID 124636-7
    ISSN 1865-7125 ; 0341-0382 ; 0341-0471 ; 0939-5075
    ISSN (online) 1865-7125
    ISSN 0341-0382 ; 0341-0471 ; 0939-5075
    DOI 10.1515/znc-1983-3-424
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  7. Article ; Online: Evaluating the ameliorative potential of plant flavonoids and their nanocomposites in bleomycin induced idiopathic pulmonary fibrosis

    Srabani Kar / Silpak Biswas / Ena Ray Banerjee

    Biomedical Research and Therapy, Vol 3, Iss 7, Pp 707-

    2016  Volume 722

    Abstract: Introduction: Pulmonary Fibrosis can severely disrupt lung function. The cause of Idiopathic Pulmonary Fibrosis (IPF) is still unclear. The treatment currently available for IPF provides minimal benefits. Bleomycin is used in research to induce ... ...

    Abstract Introduction: Pulmonary Fibrosis can severely disrupt lung function. The cause of Idiopathic Pulmonary Fibrosis (IPF) is still unclear. The treatment currently available for IPF provides minimal benefits. Bleomycin is used in research to induce experimental pulmonary fibrosis in animals. Plant flavonoids such as fisetin and curcumin are known for their anti-inflammatory properties. The aim was to demonstrate the path of physiological changes in bleomycin induced IPF and to further evaluate the reversal and ameliorative potentials of some plant flavonoids and their nanocomposites. Bleomycin administration is known to damage the lung epithelial cells resulting in lower cell numbers. Results: Our colony forming assay in BALF also demonstrated a reduction (1.65 fold) in colony-forming unit after bleomycin administration. The number of colonies increased upon treatment with fisetin(1.20 fold) and curcumin (1.13 fold) respectively. MTT proliferation assay demonstrated a significant increase in the number of viable cells upon treatment with curcumin (1.42 fold) and fisetin (1.74 fold) respectively, in the lung. The NBT assay demonstrated a significant effect of curcumin in BALF, lung and peripheral blood. A significant effect of fisetin was observed in BALF and peripheral blood. Conclusion: These findings were also supported by the histological analyses of the lung samples. Curcumin showed highestpotential for the treatment of IPF and fisetin showed its ameliorative effect. [Biomed Res Ther 2016; 3(7.000): 707-722]
    Keywords Idiopathic pulmonary fibrosis ; bleomycin ; flavonoids ; nanocomposites ; anti-inflammatory ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2016-07-01T00:00:00Z
    Publisher BioMedPress
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Effects of intrathecal dexmedetomidine as an additive to low-dose bupivacaine in patients undergoing transurethral resection of prostate.

    Chattopadhyay, Ipsita / Banerjee, Sumantra Sarathi / Jha, Amarendra Kumar / Basu, Srabani

    Indian journal of anaesthesia

    2017  Volume 61, Issue 12, Page(s) 1002–1008

    Abstract: Background and aims: In patients undergoing transurethral resection of prostate (TURP), it is vital to restrict the level of block to T: Methods: The study was designed as a prospective, double-blind, randomised trial that included sixty patients of ... ...

    Abstract Background and aims: In patients undergoing transurethral resection of prostate (TURP), it is vital to restrict the level of block to T
    Methods: The study was designed as a prospective, double-blind, randomised trial that included sixty patients of American Society of Anesthesiologists Grade I-III scheduled for TURP. They were allocated into two groups: Group I receiving only hyperbaric bupivacaine intrathecally and Group II receiving dexmedetomidine with low dose bupivacaine. The time to regression of two dermatomes from the peak sensory block level was the primary outcome of the study.
    Results: With comparable baseline and demographic attributes, both groups had similar peak sensory block levels (T
    Conclusion: Intrathecal dexmedetomidine with low-dose bupivacaine provides faster onset, prolonged sensory and motor block and reduced rescue analgesic requirement in patients undergoing TURP.
    Language English
    Publishing date 2017-12-29
    Publishing country India
    Document type Journal Article
    ZDB-ID 412570-8
    ISSN 0019-5049
    ISSN 0019-5049
    DOI 10.4103/ija.IJA_324_16
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1841: Show issues Location:
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  9. Book: Comparison of lung volume reduction surgery with medical management for emphysema

    Banerjee, Srabani

    (Technology report ; issue 48)

    2004  

    Institution Canadian Coordinating Office for Health Technology Assessment
    Author's details Srabani Banerjee ... [et al.]
    Series title Technology report ; issue 48
    MeSH term(s) Pulmonary Emphysema/surgery ; Pulmonary Emphysema/therapy ; Pneumonectomy ; Risk Factors ; Treatment Outcome
    Language English
    Size ix, 72 p. :, ill. ;, 28 cm.
    Publisher Canadian Coordinating Office for Health Technology Assessment
    Publishing place Ottawa
    Document type Book
    Note "December 2004."
    ISBN 9781894978453 ; 1894978455 ; 9781894978446 ; 1894978447
    Database Catalogue of the US National Library of Medicine (NLM)

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  10. Article: Multi-slice/helical computed tomography for lung cancer screening.

    Banerjee, Srabani

    Issues in emerging health technologies

    2003  , Issue 48, Page(s) 1–4

    Abstract: Compared to chest radiography, multislice/helical computed tomography (CT) is able to detect lung cancers of smaller size and at an earlier stage, when better outcomes may be possible. However, the rate of detecting benign lung nodules is high using ... ...

    Abstract Compared to chest radiography, multislice/helical computed tomography (CT) is able to detect lung cancers of smaller size and at an earlier stage, when better outcomes may be possible. However, the rate of detecting benign lung nodules is high using these technologies. False positives can result in undue anxiety and/or additional tests that may be invasive and expensive. Currently evidence does not exist to suggest that detecting early stage lung cancer reduces mortality. Randomized controlled trials are underway to investigate this issue. Presently, even for smokers, screening for lung cancer with multi-slice/helical CT would be premature.
    MeSH term(s) Canada ; Cost-Benefit Analysis ; Costs and Cost Analysis ; Device Approval ; False Positive Reactions ; Humans ; Lung Neoplasms/diagnostic imaging ; Lung Neoplasms/mortality ; Lung Neoplasms/prevention & control ; Mass Chest X-Ray ; Randomized Controlled Trials as Topic ; Sensitivity and Specificity ; Tomography, Spiral Computed/adverse effects ; Tomography, Spiral Computed/economics ; Tomography, Spiral Computed/methods ; Tomography, Spiral Computed/utilization
    Language English
    Publishing date 2003-06
    Publishing country Canada
    Document type Comparative Study ; Journal Article
    ZDB-ID 2272169-1
    ISSN 1488-6316
    ISSN 1488-6316
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6559: Show issues Location:
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