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  1. Article ; Online: Insights into the inhibition of protospacer integration via direct interaction between Cas2 and AcrVA5.

    Bi, Mingfang / Su, Wenjing / Li, Jiafu / Mo, Xiaobing

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 3256

    Abstract: Spacer acquisition step in CRISPR-Cas system involves the recognition and subsequent integration of protospacer by the Cas1-Cas2 complex in CRISPR-Cas systems. Here we report an anti-CRISPR protein, AcrVA5, and reveal the mechanisms by which it strongly ... ...

    Abstract Spacer acquisition step in CRISPR-Cas system involves the recognition and subsequent integration of protospacer by the Cas1-Cas2 complex in CRISPR-Cas systems. Here we report an anti-CRISPR protein, AcrVA5, and reveal the mechanisms by which it strongly inhibits protospacer integration. Our biochemical data shows that the integration by Cas1-Cas2 was abrogated in the presence of AcrVA5. AcrVA5 exhibits low binding affinity towards Cas2 and acetylates Cas2 at Lys
    MeSH term(s) CRISPR-Associated Proteins/metabolism ; CRISPR-Cas Systems
    Chemical Substances CRISPR-Associated Proteins
    Language English
    Publishing date 2024-04-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47713-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Role of Dipolar Organic Cations on Light-triggered Charge Transfer at TiO

    Zhang, Mingfang / Feng, Qingjie / Li, Sheng / Nan, Guangjun

    Chemphyschem : a European journal of chemical physics and physical chemistry

    2023  Volume 24, Issue 22, Page(s) e202300376

    Abstract: ... The ... ...

    Abstract The TiO
    Language English
    Publishing date 2023-09-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2025223-7
    ISSN 1439-7641 ; 1439-4235
    ISSN (online) 1439-7641
    ISSN 1439-4235
    DOI 10.1002/cphc.202300376
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  3. Article ; Online: Artificial intelligence and cognitive diagnosis based teaching resource recommendation algorithm

    Zhi Mao / Mingfang Li

    PeerJ Computer Science, Vol 9, p e

    2023  Volume 1594

    Abstract: In the realm of advanced technology, deep learning capabilities are harnessed to analyze and predict novel data, once it has absorbed existing information. When applied to the sphere of education, this transformative technology becomes a catalyst for ... ...

    Abstract In the realm of advanced technology, deep learning capabilities are harnessed to analyze and predict novel data, once it has absorbed existing information. When applied to the sphere of education, this transformative technology becomes a catalyst for innovation and reform, leading to advancements in teaching modes, methodologies, and curricula. In light of these possibilities, the application of deep learning technology to teaching resource recommendations is explored in this article. Within the context of the study, a bespoke recommendation algorithm for teaching resources is devised, drawing upon the integration of deep learning and cognitive diagnosis (ADCF). This intricately constructed model consists of two core elements: the Multi-layer Perceptron (MLP) and the Generalized Matrix Factorization (GMF), operating cohesively through stages of linear representation and nonlinear learning of the interaction function. The empirical analysis reveals that the ADCF model achieves 0.626 and 0.339 in the hits ratio (HR) and the Normalized Discounted Cumulative Gain (NDCG) respectively due to the traditional model, signifying its potential to add significant value to the domain of teaching resource recommendations.
    Keywords Deep learning ; Cognitive diagnosis ; Teaching resource recommendation ; Artificial intelligence ; Electronic computers. Computer science ; QA75.5-76.95
    Subject code 370 ; 401
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher PeerJ Inc.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Artificial intelligence and cognitive diagnosis based teaching resource recommendation algorithm.

    Mao, Zhi / Li, Mingfang

    PeerJ. Computer science

    2023  Volume 9, Page(s) e1594

    Abstract: In the realm of advanced technology, deep learning capabilities are harnessed to analyze and predict novel data, once it has absorbed existing information. When applied to the sphere of education, this transformative technology becomes a catalyst for ... ...

    Abstract In the realm of advanced technology, deep learning capabilities are harnessed to analyze and predict novel data, once it has absorbed existing information. When applied to the sphere of education, this transformative technology becomes a catalyst for innovation and reform, leading to advancements in teaching modes, methodologies, and curricula. In light of these possibilities, the application of deep learning technology to teaching resource recommendations is explored in this article. Within the context of the study, a bespoke recommendation algorithm for teaching resources is devised, drawing upon the integration of deep learning and cognitive diagnosis (ADCF). This intricately constructed model consists of two core elements: the Multi-layer Perceptron (MLP) and the Generalized Matrix Factorization (GMF), operating cohesively through stages of linear representation and nonlinear learning of the interaction function. The empirical analysis reveals that the ADCF model achieves 0.626 and 0.339 in the hits ratio (HR) and the Normalized Discounted Cumulative Gain (NDCG) respectively due to the traditional model, signifying its potential to add significant value to the domain of teaching resource recommendations.
    Language English
    Publishing date 2023-11-09
    Publishing country United States
    Document type Journal Article
    ISSN 2376-5992
    ISSN (online) 2376-5992
    DOI 10.7717/peerj-cs.1594
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  5. Article ; Online: Anti-BNLF2b Screening for Nasopharyngeal Cancer. Reply.

    Li, Tingdong / Ji, Mingfang / Xia, Ningshao

    The New England journal of medicine

    2023  Volume 389, Issue 19, Page(s) 1825

    MeSH term(s) Humans ; Nasopharyngeal Neoplasms/diagnosis ; Herpesvirus 4, Human ; Early Detection of Cancer ; Nasopharyngeal Carcinoma ; Mass Screening
    Language English
    Publishing date 2023-11-08
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 207154-x
    ISSN 1533-4406 ; 0028-4793
    ISSN (online) 1533-4406
    ISSN 0028-4793
    DOI 10.1056/NEJMc2311385
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    Ua VI Zs.34: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  6. Article: Association Between Psoriasis and Non-Alcoholic Fatty Liver Disease: A Two-Sample Mendelian Randomization Study.

    Li, Hong / Su, Juan / Zhu, Mingfang / Zhu, Yaqi / Zhu, Shilin

    Clinical, cosmetic and investigational dermatology

    2023  Volume 16, Page(s) 3291–3294

    Language English
    Publishing date 2023-11-14
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494852-4
    ISSN 1178-7015
    ISSN 1178-7015
    DOI 10.2147/CCID.S439115
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  7. Article ; Online: Effect of SOX2 gene on the biological characteristics of salivary adenoid cystic carcinoma cells.

    Wei, Zhuo / Zhou, Hao / Fei, Wei / Nie, Xiong / Li, Mingfang

    Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology

    2024  Volume 40, Issue 3, Page(s) 341–349

    Abstract: Objectives: This study aimed to investigate the effect of transfecting SOX2-shRNA vector lentivirus to SACC cell lines on the biological behavior of salivary adenoid cystic carcinoma (SACC)-LM and SACC-83.: Methods: Three types of SOX2-shRNA ... ...

    Abstract Objectives: This study aimed to investigate the effect of transfecting SOX2-shRNA vector lentivirus to SACC cell lines on the biological behavior of salivary adenoid cystic carcinoma (SACC)-LM and SACC-83.
    Methods: Three types of SOX2-shRNA lentiviral vectors (817, 818, and 819) were constructed and transfected successfully. The shRNA with the best inhibitory effect was screened out and transfected into SACC cells. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expressions of Survivin, E-cadherin, and N-cadherin of SACC-LM and SACC-83. CCK-8 and flow cytometry were used to detect SACC-LM and SACC-83 proliferation and apoptosis. Cell scratch test and Transwell method were used to detect the migration and invasion capabilities of SACC-LM and SACC-83.
    Results: SOX2-shRNA-819 had the best interference effect among the three lentiviruses. After transfecting SOX2-shRNA-819 into SACC-LM and SACC-83, the expressions of SOX2, Survivin, and N-cadherin were significantly reduced, and that of E-cadherin was significantly increased (
    Conclusions: shRNA interference technology reduced SOX2 expression while downregulating Survivin expression. These two expressions may be related. Low SOX2 expression inhibits the proliferation, migration, and invasion of SACC cells and promotes the apoptosis of SACC cells. SOX2 may be involved in the epithelial⁃mesenchymal transition process. This study provided a relevant theoretical basis for targeting SOX2 gene therapy in SACC.
    Language Chinese
    Publishing date 2024-04-10
    Publishing country China
    Document type Journal Article
    ZDB-ID 1202342-5
    ISSN 2618-0456 ; 1000-1182
    ISSN (online) 2618-0456
    ISSN 1000-1182
    DOI 10.7518/hxkq.2022.03.015
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4073: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article ; Online: Long-term effect of acetate and biochar addition on enrichment and activity of denitrifying anaerobic methane oxidation microbes.

    Ding, Jing / Qin, Fan / Li, Changxin / Tang, Mingfang

    Chemosphere

    2023  Volume 338, Page(s) 139642

    Abstract: The denitrifying anaerobic methane oxidation (DAMO) process plays a crucial role in the global carbon/nitrogen cycles and methane emission control, and also has application potential in biological wastewater treatment. However, given that DAMO microbes ... ...

    Abstract The denitrifying anaerobic methane oxidation (DAMO) process plays a crucial role in the global carbon/nitrogen cycles and methane emission control, and also has application potential in biological wastewater treatment. However, given that DAMO microbes are susceptible to external conditions such as additional carbon source in the system, it is essential to evaluate the effect of alternative carbon substance on the enrichment efficiency and metabolic activity of DAMO microbes. To this end, this study investigated the effect of acetate (0.1 mmol/L-R2, 0.5 mmol/L-R3) and biochar addition (R4) on the enrichment and activity of DAMO microbes. The long-term operation showed that the NO
    MeSH term(s) Nitrates/metabolism ; Anaerobiosis ; Methane/metabolism ; Extracellular Polymeric Substance Matrix/metabolism ; Denitrification ; Wastewater ; Oxidation-Reduction ; Nitrogen/metabolism ; Carbon ; Acetates ; Bioreactors/microbiology ; Nitrites/metabolism
    Chemical Substances Nitrates ; biochar ; Methane (OP0UW79H66) ; Wastewater ; Nitrogen (N762921K75) ; Carbon (7440-44-0) ; Acetates ; Nitrites
    Language English
    Publishing date 2023-07-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2023.139642
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    Zs.A 2540: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  9. Article: Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics.

    Wu, Hangsha / Lv, Yue / Tang, Rui / Zhao, Mingfang / Li, Yafei / Wei, Feiyang / Li, Changyu / Ge, Weihong / Du, Weifeng

    International journal of analytical chemistry

    2024  Volume 2024, Page(s) 2109127

    Abstract: Radix Dipsaci (RD) is the dry root of ... ...

    Abstract Radix Dipsaci (RD) is the dry root of the
    Language English
    Publishing date 2024-02-07
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 2494714-3
    ISSN 1687-8779 ; 1687-8760
    ISSN (online) 1687-8779
    ISSN 1687-8760
    DOI 10.1155/2024/2109127
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  10. Article ; Online: Industrial characteristics and drivers of urban carbon cycle

    Ling Li / Yuan Shen / Yuejing Rong / Xiao Fu / Mingfang Tang / Hongbing Deng / Gang Wu

    Ecological Indicators, Vol 158, Iss , Pp 111463- (2024)

    From an analysis of typical cities in China

    1481  

    Abstract: The urban carbon cycle is an important part of the global carbon cycle. Clarifying the processes and drivers of the urban carbon cycle is essential for achieving urban carbon neutrality. Research on the urban carbon cycle under various industrial ... ...

    Abstract The urban carbon cycle is an important part of the global carbon cycle. Clarifying the processes and drivers of the urban carbon cycle is essential for achieving urban carbon neutrality. Research on the urban carbon cycle under various industrial characteristics is still insufficient. In this study, four typical Chinese cities are selected and their carbon cycle processes and influencing factors are analysed. Here we propose an urban carbon neutral rate to evaluate the balance between urban carbon input and carbon output. Through Grey correlation analysis, we explore the main influencing factors of urban carbon neutral rate. The results show that the carbon neutral rate of Chinese cities is uneven and polarised. Heavy industrial cities face major challenges in urban carbon neutrality. In addition, the main factors affecting the carbon neutral rate of cities of different industrial types are not the same. The formulation of urban carbon neutral policies and measures should be differentiated and focused according to the industrial characteristics of cities.
    Keywords Urban carbon cycle ; Urban carbon emissions ; Carbon neutral rate ; Carbon neutrality ; Urban carbon accounting ; Ecology ; QH540-549.5
    Subject code 720
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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