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  1. Article ; Online: Analysis of lithotripsy efficiency and stone retropulsion displacement according to pulse characteristics of Ho: YAG laser with Moses effect.

    Kim, Kanghae / Seo, Young-Seok / Chung, Phil-Sang / Eom, Joo Beom

    Lasers in surgery and medicine

    2023  Volume 56, Issue 2, Page(s) 197–205

    Abstract: Background and objectives: Compared to the conventional Ho: YAG laser, a Ho: YAG laser device has been reported that has a Moses effect to reduce stone retropulsion and increase lithotripsy efficiency. The principle of this equipment is to convert a ... ...

    Abstract Background and objectives: Compared to the conventional Ho: YAG laser, a Ho: YAG laser device has been reported that has a Moses effect to reduce stone retropulsion and increase lithotripsy efficiency. The principle of this equipment is to convert a single laser pulse into two pulses. Most studies on such lasers are limited to lithotripsy efficiency and the prevention of stone retropulsion; studies according to each pulse condition have not been performed. Therefore, the purpose of this study was to quantify the bubble shape, lithotripsy efficiency, and stone retropulsion displacement in a ureteral phantom according to the modulation of the first pulse characteristics of the Moses effect laser under conditions that maintained the total energy and repetition rate.
    Material and methods: In this study, a Ho: YAG laser system (Holinwon Pro, Wontech Inc., Korea) with an emission wavelength of 2.10 μm and a Moses effect was used. To verify the Moses effect based on the changes in the pulse, a water tank was fabricated, and the ureteral phantom was manufactured in a structure that could be easily installed in the water tank. Additionally, a spherical artificial stone in the ureteral phantom was prepared by mixing calcined gypsum (Cacinated Gypsum) and water at a ratio of 3:1. In the ureteral phantom, a high-speed camera (FASTCAM NOVA S12, Photron Inc.) and visible light were used to record pulse-dependent image analysis of bubbles and stone retropulsion.
    Result: After mounting the artificial stone in the ureteral phantom, the pulse duration and energy of the first pulse of the Moses effect laser were varied; 30 laser shots for 3 s at a repetition rate of 10 Hz were applied to quantify the lithotripsy efficiency and stone retropulsion displacement, and the experimental values were compared. The fragmentation efficiency was confirmed by measuring the mass before and after the laser pulse application, the original position of the stone retropulsion displacement, and the distance moved. The minimum value of stone retropulsion displacement appeared when the pulse duration of the first pulse was 300 μs, the pulse energy was 100 mJ, and the value was approximately 0.28 mm. The highest fragmentation efficiency was observed under the same conditions, and the mass loss of the artificial stone at that time was approximately 3.7 mg.
    Conclusion: Quantitative indices, such as lithotripsy efficiency and stone retropulsion displacement, were confirmed using ultrahigh-speed cameras to determine the effect of the first pulse energy and duration of the Ho: YAG laser with the Moses effect on stone removal. It was confirmed that the longer the duration of the primary pulse and the lower the energy, the higher the fragmentation efficiency. In this study, the possibility of manufacturing a laser with an optimal stone-removal effect was confirmed according to the first-pulse condition of the laser with the Moses effect.
    MeSH term(s) Lasers, Solid-State/therapeutic use ; Calcium Sulfate ; Lithotripsy, Laser ; Lithotripsy ; Water
    Chemical Substances Calcium Sulfate (WAT0DDB505) ; Water (059QF0KO0R)
    Language English
    Publishing date 2023-11-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23743
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  2. Article ; Online: Photobiomodulation Recovers the Submandibular Gland in Vismodegib-Treated Rats.

    Kim, Yoon-Ah / Abueva, Celine / Padalhin, Andrew / Park, So Young / Lee, Ha Young / Ryu, Hyun-Seok / Chung, Phil-Sang / Woo, Seung Hoon

    Photobiomodulation, photomedicine, and laser surgery

    2024  Volume 42, Issue 2, Page(s) 159–167

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Rats ; Animals ; Submandibular Gland/metabolism ; Zinc Finger Protein GLI1/metabolism ; Zinc Finger Protein GLI1/pharmacology ; Low-Level Light Therapy ; Signal Transduction ; Anilides ; Pyridines
    Chemical Substances HhAntag691 ; Zinc Finger Protein GLI1 ; Anilides ; Pyridines
    Language English
    Publishing date 2024-02-02
    Publishing country United States
    Document type Journal Article
    ISSN 2578-5478
    ISSN (online) 2578-5478
    DOI 10.1089/photob.2023.0063
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  3. Article ; Online: Antiseptic, Hemostatic, and Wound Activity of Poly(vinylpyrrolidone)-Iodine Gel with Trimethyl Chitosan.

    Padalhin, Andrew / Ryu, Hyun Seok / Yoo, Seung Hyeon / Abueva, Celine / Seo, Hwee Hyon / Park, So Young / Chung, Phil-Sang / Woo, Seung Hoon

    International journal of molecular sciences

    2024  Volume 25, Issue 4

    Abstract: Wound management practices have made significant advancements, yet the search for improved antiseptics persists. In our pursuit of solutions that not only prevent infections but also address broader aspects of wound care, we investigated the impact of ... ...

    Abstract Wound management practices have made significant advancements, yet the search for improved antiseptics persists. In our pursuit of solutions that not only prevent infections but also address broader aspects of wound care, we investigated the impact of integrating trimethyl chitosan (TMC) into a widely used poly(vinylpyrrolidone)-iodine gel (PVP-I gel). Our study assessed the antimicrobial efficacy of the PVP gel with TMC against
    MeSH term(s) Rats ; Animals ; Anti-Infective Agents, Local/pharmacology ; Povidone-Iodine/pharmacology ; Chitosan/pharmacology ; Iodine ; Hemostatics/pharmacology ; Methicillin-Resistant Staphylococcus aureus ; Anti-Infective Agents/pharmacology
    Chemical Substances Anti-Infective Agents, Local ; Povidone-Iodine (85H0HZU99M) ; Chitosan (9012-76-4) ; Iodine (9679TC07X4) ; Hemostatics ; Anti-Infective Agents
    Language English
    Publishing date 2024-02-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25042106
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  4. Article ; Online: Evaluation of the safety and efficacy of long pulsed Nd: YAG laser in the treatment of vascular lesions

    Rhee, Yun-Hee / Ryu, Han-Yong / Ahn, Jin-Chul / Chung, Phil-Sang

    Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology

    2022  Volume 24, Issue 1-5, Page(s) 40–47

    Abstract: Long-pulsed neodymium:yttrium-aluminum-garnet (Nd:YAG) lasers have recently been used for the treatment of vascular lesions refractory to conventional vascular lasers. The aim of this study was to evaluate the clinical efficacy and safety of long-pulsed ... ...

    Abstract Long-pulsed neodymium:yttrium-aluminum-garnet (Nd:YAG) lasers have recently been used for the treatment of vascular lesions refractory to conventional vascular lasers. The aim of this study was to evaluate the clinical efficacy and safety of long-pulsed Nd:YAG laser treatment for vascular disorders. Laser irradiation was performed using two approaches: the 532 nm Nd:YAG laser was used to irradiate the dorsal skin fold in mice and the 1064 nm Nd:YAG laser was used to irradiate the leg of mice without skin incision. The specimens were observed immediately after laser treatment using a laser Doppler perfusion imaging system. Red blood cell (RBC) extravasation and hemorrhage were observed using the hematoxylin and eosin stain. The diameter of blood vessel under 30 μm was disrupted with a laser pulse at a fluence of 12 J/cm
    MeSH term(s) Mice ; Animals ; Lasers, Solid-State/adverse effects ; Dermatologic Surgical Procedures ; Neodymium ; Treatment Outcome
    Chemical Substances Neodymium (2I87U3734A)
    Language English
    Publishing date 2022-07-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2146360-8
    ISSN 1476-4180 ; 1476-4172
    ISSN (online) 1476-4180
    ISSN 1476-4172
    DOI 10.1080/14764172.2022.2100913
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Oral ulcer treatment using human tonsil-derived mesenchymal stem cells encapsulated in trimethyl chitosan hydrogel: an animal model study.

    Ryu, Hyun Seok / Abueva, Celine / Padalhin, Andrew / Park, So Young / Yoo, Seung Hyeon / Seo, Hwee Hyon / Chung, Phil-Sang / Woo, Seung Hoon

    Stem cell research & therapy

    2024  Volume 15, Issue 1, Page(s) 103

    Abstract: Background: Oral ulcers are a common side effect of chemotherapy and affect patients' quality of life. While stem cell transplantation is a potential treatment for oral ulcers, its efficacy is limited as the stem cells tend to remain in the affected ... ...

    Abstract Background: Oral ulcers are a common side effect of chemotherapy and affect patients' quality of life. While stem cell transplantation is a potential treatment for oral ulcers, its efficacy is limited as the stem cells tend to remain in the affected area for a short time. This study aims to develop a treatment for oral ulcers by using trimethyl chitosan (TMC) hydrogel with human tonsil-derived stem cells (hTMSCs) to increase the therapeutic effect of stem cells and investigate their effectiveness.
    Methods: Animals were divided into four experimental groups: Control, TMC hydrogel, hTMSCs, and hTMSCs loaded in TMC hydrogel (Hydrogel + hTMSCs) (each n = 8). Oral ulcers were chemically induced by anesthetizing the rats followed by injection of dilute acetic acid in the right buccal mucosa. After confirming the presence of oral ulcers in the animals, a single subcutaneous injection of 100 µL of each treatment was applied to the ulcer area. Histological analyses were performed to measure inflammatory cells, oral mucosal thickness, and fibrosis levels. The expression level of inflammatory cytokines was also measured using RT-PCR to gauge therapeutic the effect.
    Results: The ulcer size was significantly reduced in the TMC hydrogel + hTMSCs group compared to the control group. The stem cells in the tissue were only observed until Day 3 in the hTMSCs treated group, while the injected stem cells in the TMC Hydrogel + hTMSCs group were still present until day 7. Cytokine analysis related to the inflammatory response in the tissue confirmed that the TMC Hydrogel + hTMSCs treated group demonstrated superior wound healing compared to other experimental groups.
    Conclusion: This study has shown that the adhesion and viability of current stem cell therapies can be resolved by utilizing a hydrogel prepared with TMC and combining it with hTMSCs. The combined treatment can promote rapid healing of oral cavity wounds by enhancing anti-inflammatory effects and expediting wound healing. Therefore, hTMSC loaded in TMC hydrogel was the most effective wound-healing approach among all four treatment groups prolonging stem cell survival. However, further research is necessary to minimize the initial inflammatory response of biomaterials and assess the safety and long-term effects for potential clinical applications.
    MeSH term(s) Humans ; Rats ; Animals ; Oral Ulcer/therapy ; Ulcer ; Chitosan ; Hydrogels ; Palatine Tonsil ; Quality of Life ; Models, Animal ; Cytokines ; Mesenchymal Stem Cells
    Chemical Substances Chitosan (9012-76-4) ; Hydrogels ; Cytokines
    Language English
    Publishing date 2024-04-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2548671-8
    ISSN 1757-6512 ; 1757-6512
    ISSN (online) 1757-6512
    ISSN 1757-6512
    DOI 10.1186/s13287-024-03694-4
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  6. Article ; Online: Analysis of penetration depth and healing process of a needleless laser lancet using swept-source optical coherence tomography.

    Mun, Do Hyun / Chung, Phil-Sang / Woo, Seung Hoon / Eom, Joo Beom

    Lasers in surgery and medicine

    2022  

    Abstract: Background and objectives: Needleless laser lancets have been used to reduce needle pain in people who undertake self-monitoring of blood glucose (SMBG). Although clinical trials have been conducted to understand the degree of pain associated with the ... ...

    Abstract Background and objectives: Needleless laser lancets have been used to reduce needle pain in people who undertake self-monitoring of blood glucose (SMBG). Although clinical trials have been conducted to understand the degree of pain associated with the developed laser blood sampling device, it is only judged by the patients' subjective judgment. This study aimed to analyze the performance and healing process of a needleless laser lancet. In particular, the penetration depth and healing process of a conventional lancet and a needleless laser lancet were quantitatively analyzed using a swept-source optical coherence tomography (SS-OCT) system.
    Materials and methods: To reduce unnecessary animal sacrifice, pig skin was used to establish and set the conditions for needleless laser lancets (wavelength of 2.94 μm; LMT-5000; Lameditech), and the penetration depth and healing process were quantitatively assessed at 0, 6, 9, and 16 h after laser (energy, 150-270 mJ in stages; voltage, 7.4 V
    Results: Four 7-week-old male BALB/c nude mice were tested at intensity levels of 1-3 using a needleless laser lancet and compared with the experimental values using a conventional lancet. After the lancet test, the diameter and depth of the puncture were measured using SS-OCT and the healing process was confirmed using SS-OCT and pathological slides. The puncture diameter of the needleless laser lancet was about 0.5 mm regardless of the level. In addition, the average depths at Levels 1, 2, and 3 of the measured needleless laser lancet were approximately 1.05, 1.18, and 1.52 mm, respectively, and the last common lancet was approximately 0.9 mm. In addition, the healing process of the needleless laser lancet and conventional lancet was confirmed through the SS-OCT system.
    Conclusion: After using the needleless laser lancet, quantitative indicators such as the diameter of puncture, penetration depth, and healing process were confirmed through two-dimensional and three-dimensional images of the homemade SS-OCT system and microscopic imaging of pathological slides.
    Language English
    Publishing date 2022-11-02
    Publishing country United States
    Document type Case Reports
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23616
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  7. Article ; Online: Biochemical and Pain Comparisons Between the Laser Lancing Device and Needle Lancets for Capillary Blood Sampling: A Randomized Control Trial.

    Yoo, Won Sang / Min, Junwon / Chung, Phil-Sang / Woo, Seung Hoon

    Lasers in surgery and medicine

    2020  Volume 53, Issue 3, Page(s) 316–323

    Abstract: Background and objectives: Patients around the world use a lancing device to perform self-monitoring of blood sugar (SMBG). However, there are always fears of needles and pain. Therefore, less painful devices are being developed. The purpose of this ... ...

    Abstract Background and objectives: Patients around the world use a lancing device to perform self-monitoring of blood sugar (SMBG). However, there are always fears of needles and pain. Therefore, less painful devices are being developed. The purpose of this study was to compare the usefulness and safety of a laser lancing device (without a needle) to a conventional needle lancet (with a needle) for capillary blood sampling.
    Study design/materials and methods: A total of 40 healthy subjects were enrolled in the study. Capillary blood was collected from a laser lancing device (without a needle) and a conventional needle lancet (with a needle) on opposite fingers, the choice of which was randomly selected. The laser lancing device (LMT-3000) uses a 2940 nm mono-pulse laser, a radiation field of 350 μm, laser energy of 210 mJ, and a 3.7 V battery. One week later, capillary blood was obtained by switching the devices and fingers. The biochemical measurements and pain were compared between the two groups. Puncture pain was measured on a pain scale from 0 to 10.
    Result: All patients were tested with both a laser lancing device and a conventional needle lancet. In the biochemical analysis, the blood glucose level was 103.21 ± 17.20 mg/dl in laser lancing device group and 102.25 ± 22.44 mg/dl in the conventional needle lancet group, and there were no significant differences between the two groups (P = 0.940). The pH, CO
    Conclusion: There was no difference in biochemical results between the laser lancing device group and the conventional needle lancet group. The laser lancing device demonstrated comparatively lower pain than the conventional needle lancet. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.
    MeSH term(s) Blood Specimen Collection ; Humans ; Lasers ; Needles ; Pain/etiology ; Pain Measurement
    Language English
    Publishing date 2020-07-07
    Publishing country United States
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23298
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  8. Article ; Online: Aqueous extract of

    Rhee, Yun-Hee / Yoo, Sang-Woo / Lee, Seong / Chung, Phil-Sang

    Food science and biotechnology

    2018  Volume 28, Issue 1, Page(s) 253–260

    Abstract: This study aimed to evaluate the effect of aqueous extract ... ...

    Abstract This study aimed to evaluate the effect of aqueous extract of
    Language English
    Publishing date 2018-07-11
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2000008-X
    ISSN 2092-6456 ; 1226-7708
    ISSN (online) 2092-6456
    ISSN 1226-7708
    DOI 10.1007/s10068-018-0434-x
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  9. Article ; Online: Effect of Photobiomodulation on Restoration of Ionization Radiation-Induced Thyroid Dysfunction Through p53 and Retinoblastoma Signaling.

    Yoo, Won Sang / Rhee, Yun-Hee / Woo, Seung Hoon / Shin, KyoChul / Ahn, Jin-Chul / Chung, Hyung Kyung / Chung, Phil-Sang

    Photobiomodulation, photomedicine, and laser surgery

    2021  Volume 39, Issue 4, Page(s) 254–264

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Animals ; Cell Survival ; Mice ; Retinal Neoplasms ; Retinoblastoma/radiotherapy ; Thyroid Gland ; Tumor Suppressor Protein p53/genetics
    Chemical Substances Tumor Suppressor Protein p53
    Language English
    Publishing date 2021-04-12
    Publishing country United States
    Document type Journal Article
    ISSN 2578-5478
    ISSN (online) 2578-5478
    DOI 10.1089/photob.2020.4936
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  10. Article ; Online: Electrochemical lipolysis of subcutaneous adipose tissue in a porcine animal model.

    Bae, Jun Sang / Abueva, Celine D G / Park, Geun Hwan / Kim, Sehwan / Mo, Ji-Hun / Ryu, Hyun Seok / Chung, Phil-Sang / Woo, Seung Hoon

    Lasers in surgery and medicine

    2022  Volume 55, Issue 2, Page(s) 200–207

    Abstract: Objectives: There is a considerable demand for noninvasive low-cost fat reduction methods with fewer side effects and shorter recovery times. This study aims to develop a fat-reduction method through electrochemical lipolysis of subcutaneous adipocytes ... ...

    Abstract Objectives: There is a considerable demand for noninvasive low-cost fat reduction methods with fewer side effects and shorter recovery times. This study aims to develop a fat-reduction method through electrochemical lipolysis of subcutaneous adipocytes using needle-based electrodes, body tissue fluids, and electrical current application.
    Methods: Electrochemical lipolysis was performed by inserting a 4-pin needle electrode connected to a DC power supply into the pig's abdomen. Applied electrical current (0.5 and 1 mA) and treatment time (5 or 10 minutes) were varied systematically. Ultrasound imaging was performed before and after treatment to determine changes in fat thickness. Tissue samples were collected at 0, 2, and 4 weeks posttreatment for histological evaluation to determine the mechanism of action and the procedure's efficacy.
    Results: Electrochemical subcutaneous adipose tissue lipolysis in a porcine model was achieved through hydrolysis of physiologic fluid within the vicinity of the inserted electrode where an electric current is applied, leading to localized disruption of fat cell membranes and necrosis. Electric current configuration 1.0 mA showed more pronounced lipolysis effects applied for 10 minutes, significantly decreasing adipocyte content per treatment area. The electrochemical treatment method also stimulates collagen synthesis, which helps reduce fat.
    Conclusions: Electrochemical lipolysis is a potential new noninvasive localized technique to reduce fat. The treatment method induces fat cell necrosis via in situ reduction-oxidation reaction by the electrochemical activation of physiologic fluid in the surrounding tissue. Electrochemical lipolysis is a simple, low-cost, fat-reducing treatment method without harmful side effects.
    MeSH term(s) Swine ; Animals ; Lipolysis/physiology ; Subcutaneous Fat/pathology ; Adipocytes/metabolism ; Adipocytes/pathology ; Models, Animal ; Necrosis/metabolism ; Necrosis/pathology ; Adipose Tissue
    Language English
    Publishing date 2022-12-09
    Publishing country United States
    Document type Case Reports ; Research Support, Non-U.S. Gov't
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23621
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