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Article ; Online: Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12.

Sommerfeld, Florian / Weyersberg, Laura / Vatter, Petra / Hessling, Martin

2024 Volume 17, Issue 1, Page(s) 3

Abstract: Objective: It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by ... ...

Abstract	Objective: It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by UVA, violet or blue light. Test virus is the bacteriophage PhiX174 in the photosensitizer-free SM buffer and DMEM-F12, which contains the known photosensitizer riboflavin. Results: The determined PhiX174 D90 doses in SM buffer and DMEM were 36.8 J/cm² and 13.6 J/cm² at 366 nm, 153.6 J/cm² and 129.1 J/cm² at 408 nm and 4988 J/cm² and 2477.1 J/cm² at 455 nm, respectively. It can be concluded that the medium has a large influence on the results. This might be caused by the photosensitizer riboflavin in DMEM-F12. As riboflavin is a key component in many cell culture media, irradiation experiments with viruses in cell culture media should be avoided if the investigation of intrinsical photoinactivation properties of viruses is aimed for.
MeSH term(s)	Photosensitizing Agents/pharmacology ; Bacteriophages ; Light ; Ultraviolet Rays ; Riboflavin/pharmacology
Chemical Substances	Photosensitizing Agents ; Riboflavin (TLM2976OFR)
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2413336-X
ISSN	1756-0500 ; 1756-0500
ISSN (online)	1756-0500
ISSN	1756-0500
DOI	10.1186/s13104-023-06658-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12

Florian Sommerfeld / Laura Weyersberg / Petra Vatter / Martin Hessling

BMC Research Notes, Vol 17, Iss 1, Pp 1-

2024 Volume 7

Abstract: Abstract Objective It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under ... ...

Abstract	Abstract Objective It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by UVA, violet or blue light. Test virus is the bacteriophage PhiX174 in the photosensitizer-free SM buffer and DMEM-F12, which contains the known photosensitizer riboflavin. Results The determined PhiX174 D90 doses in SM buffer and DMEM were 36.8 J/cm² and 13.6 J/cm² at 366 nm, 153.6 J/cm² and 129.1 J/cm² at 408 nm and 4988 J/cm² and 2477.1 J/cm² at 455 nm, respectively. It can be concluded that the medium has a large influence on the results. This might be caused by the photosensitizer riboflavin in DMEM-F12. As riboflavin is a key component in many cell culture media, irradiation experiments with viruses in cell culture media should be avoided if the investigation of intrinsical photoinactivation properties of viruses is aimed for.
Keywords	PhiX174 ; UVA ; Visible irradiation ; Radiation inactivation ; Blue light ; Violet light ; Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
Subject code	535
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Thesis: Funktionelle Charakterisierung von vier Varianten der murinen Kinase G-Protein-gekoppelter Rezeptoren GRK6

Vatter, Petra

2000

Author's details	vorgelegt von Petra Vatter
Language	German
Size	II, 169 Bl., Ill., graph. Darst., 30 cm
Publishing country	Germany
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Ulm, Univ., Diss., 2000
HBZ-ID	HT013067575
Database	Catalogue ZB MED Medicine, Health

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2001 E 2012	order for loan	Magazin

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Article ; Online: UV radiation sensitivity of bacteriophage PhiX174 - A potential surrogate for SARS-CoV-2 in terms of radiation inactivation.

Weyersberg, Laura / Sommerfeld, Florian / Vatter, Petra / Hessling, Martin

AIMS microbiology

2023 Volume 9, Issue 3, Page(s) 431–443

Abstract: To minimize health risks, surrogates are often employed to reduce experiments with pathogenic microorganisms and the associated health risk. Due to structural similarities between the enveloped RNA-viruses SARS-CoV-2 and Phi6, the latter has been ... ...

Abstract	To minimize health risks, surrogates are often employed to reduce experiments with pathogenic microorganisms and the associated health risk. Due to structural similarities between the enveloped RNA-viruses SARS-CoV-2 and Phi6, the latter has been established as a nonpathogenic coronavirus surrogate for many applications. However, large discrepancies in the UV log-reduction doses between SARS-CoV-2 and Phi6 necessitate the search for a better surrogate for UV inactivation applications. A literature study provided the bacteriophage PhiX174 as a potentially more suitable nonpathogenic coronavirus surrogate candidate. In irradiation experiments, the sensitivity of PhiX174 was investigated upon exposure to UV radiation of wavelengths 222 nm (Far-UVC), 254 nm (UVC), 302 nm (broad-band UVB), 311 nm (narrow-band UVB) and 366 nm (UVA) using a plaque assay. The determined log-reduction doses for PhiX174 were 1.3 mJ/cm
Language	English
Publishing date	2023-05-05
Publishing country	United States
Document type	Journal Article
ISSN	2471-1888
ISSN (online)	2471-1888
DOI	10.3934/microbiol.2023023
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The impact of far-UVC radiation (200-230 nm) on pathogens, cells, skin, and eyes - a collection and analysis of a hundred years of data.

Hessling, Martin / Haag, Robin / Sieber, Nicole / Vatter, Petra

GMS hygiene and infection control

2021 Volume 16, Page(s) Doc07

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2021-02-16
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2717852-3
ISSN	2196-5226
ISSN	2196-5226
DOI	10.3205/dgkh000378
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Article ; Online: Blue light inactivation of the enveloped RNA virus Phi6.

Vatter, Petra / Hoenes, Katharina / Hessling, Martin

BMC research notes

2021 Volume 14, Issue 1, Page(s) 187

Abstract: Objective: Ultraviolet radiation is known for its antimicrobial properties but unfortunately, it could also harm humans. Currently, disinfection techniques against SARS-CoV-2 are being sought that can be applied on air and surfaces and which do not pose ...

Abstract	Objective: Ultraviolet radiation is known for its antimicrobial properties but unfortunately, it could also harm humans. Currently, disinfection techniques against SARS-CoV-2 are being sought that can be applied on air and surfaces and which do not pose a relevant thread to humans. In this study, the bacteriophage phi6, which like SARS-CoV-2 is an enveloped RNA virus, is irradiated with visible blue light at a wavelength of 455 nm. Results: For the first time worldwide, the antiviral properties of blue light around 455 nm can be demonstrated. With a dose of 7200 J/cm
MeSH term(s)	Antiviral Agents ; COVID-19 ; Humans ; Light ; SARS-CoV-2 ; Ultraviolet Rays ; Virus Inactivation
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-05-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2413336-X
ISSN	1756-0500 ; 1756-0500
ISSN (online)	1756-0500
ISSN	1756-0500
DOI	10.1186/s13104-021-05602-y
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Article ; Online: UVC, UVB and UVA susceptibility of Phi6 and its suitability as a SARS-CoV-2 surrogate.

Weyersberg, Laura / Klemens, Eva / Buehler, Jule / Vatter, Petra / Hessling, Martin

AIMS microbiology

2022 Volume 8, Issue 3, Page(s) 278–291

Abstract: For SARS-CoV-2 disinfection systems or applications that are based on UVC, UVB or UVA irradiation, it would be desirable to have a SARS-CoV-2 surrogate for tests and development, which does not require a laboratory with a high biosafety level. The ... ...

Abstract	For SARS-CoV-2 disinfection systems or applications that are based on UVC, UVB or UVA irradiation, it would be desirable to have a SARS-CoV-2 surrogate for tests and development, which does not require a laboratory with a high biosafety level. The bacteriophage Phi 6, an enveloped RNA virus like coronaviruses, is an obvious candidate for such a surrogate. In this study, UVC, UVB and UVA log-reduction doses for Phi6 are determined by plaque assay. Log-reduction doses for SARS-CoV-2 are retrieved from a literature research. Because of a high variability of the published results, median log-reduction doses are determined for defined spectral ranges and compared to Phi6 data in the same intervals. The measured Phi6 log-reduction doses for UVC (254 nm), UVB (311 nm) and UVA (365 nm) are 31.7, 980 and 14 684 mJ/cm
Language	English
Publishing date	2022-07-08
Publishing country	United States
Document type	Journal Article
ISSN	2471-1888
ISSN (online)	2471-1888
DOI	10.3934/microbiol.2022020
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Ultraviolet irradiation doses for coronavirus inactivation - review and analysis of coronavirus photoinactivation studies.

Heßling, Martin / Hönes, Katharina / Vatter, Petra / Lingenfelder, Christian

GMS hygiene and infection control

2020 Volume 15, Page(s) Doc08

Abstract: Background: ...

Abstract	Background:
Keywords	covid19
Language	English
Publishing date	2020-05-14
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2717852-3
ISSN	2196-5226
ISSN	2196-5226
DOI	10.3205/dgkh000343
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Article ; Online: Photoinactivation of the Coronavirus Surrogate phi6 by Visible Light.

Vatter, Petra / Hoenes, Katharina / Hessling, Martin

Photochemistry and photobiology

2020 Volume 97, Issue 1, Page(s) 122–125

Abstract: To stop the coronavirus spread, new inactivation approaches are being sought that can also be applied in the presence of humans or even on humans. Here, we investigate the effect of visible violet light with a wavelength of 405 nm on the coronavirus ... ...

Abstract	To stop the coronavirus spread, new inactivation approaches are being sought that can also be applied in the presence of humans or even on humans. Here, we investigate the effect of visible violet light with a wavelength of 405 nm on the coronavirus surrogate phi6 in two aqueous solutions that are free of photosensitizers. A dose of 1300 J cm
MeSH term(s)	Humans ; Light ; Microbial Viability/radiation effects ; Reproducibility of Results ; SARS-CoV-2/growth & development ; SARS-CoV-2/radiation effects ; Viral Plaque Assay
Keywords	covid19
Language	English
Publishing date	2020-11-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	123540-0
ISSN	1751-1097 ; 0031-8655
ISSN (online)	1751-1097
ISSN	0031-8655
DOI	10.1111/php.13352
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Blue light inactivation of the enveloped RNA virus Phi6

Petra Vatter / Katharina Hoenes / Martin Hessling

BMC Research Notes, Vol 14, Iss 1, Pp 1-

2021 Volume 5

Abstract: Abstract Objective Ultraviolet radiation is known for its antimicrobial properties but unfortunately, it could also harm humans. Currently, disinfection techniques against SARS-CoV-2 are being sought that can be applied on air and surfaces and which do ... ...

Abstract	Abstract Objective Ultraviolet radiation is known for its antimicrobial properties but unfortunately, it could also harm humans. Currently, disinfection techniques against SARS-CoV-2 are being sought that can be applied on air and surfaces and which do not pose a relevant thread to humans. In this study, the bacteriophage phi6, which like SARS-CoV-2 is an enveloped RNA virus, is irradiated with visible blue light at a wavelength of 455 nm. Results For the first time worldwide, the antiviral properties of blue light around 455 nm can be demonstrated. With a dose of 7200 J/cm2, the concentration of this enveloped RNA virus can be successfully reduced by more than three orders of magnitude. The inactivation mechanism is still unknown, but the sensitivity ratio of phi6 towards blue and violet light hints towards an involvement of photosensitizers of the host cells. Own studies on coronaviruses cannot be executed, but the results support speculations about blue-susceptibility of coronaviruses, which might allow to employ blue light for infection prevention or even therapeutic applications.
Keywords	Phi6 ; Photoinactivation ; Blue light ; Coronavirus ; SARS-CoV-2 ; Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
Subject code	535
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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