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Article: Photophysical characterization of sickle cell disease hemoglobin by multi-photon microscopy.

Vigil, Genevieve D / Howard, Scott S

2015 Volume 6, Issue 10, Page(s) 4098–4104

Abstract: The photophysical properties of human sickle cell disease (SCD) Hemoglobin (Hb) is characterized by multi-photon microscopy (MPM). The intrinsic two-photon excited fluorescence (TPEF) signal associated with extracted hemoglobin was investigated and the ... ...

Abstract	The photophysical properties of human sickle cell disease (SCD) Hemoglobin (Hb) is characterized by multi-photon microscopy (MPM). The intrinsic two-photon excited fluorescence (TPEF) signal associated with extracted hemoglobin was investigated and the solidified SCD variant (HbS) was found to demonstrate broad emission peaking around 510 nm when excited at 800 nm. MPM is used to dynamically induce and image HbS gelling by photolysis of deoxygenated HbS. For comparison, photolysis conditions were applied to a healthy variant of human hemoglobin (HbA) and found to remain in solution not forming fibers. The use of this signal to study the mechanism of HbS polymerization associated with the sickling of SCD erythrocytes is discussed.
Language	English
Publishing date	2015-09-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.6.004098
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Article: Automated bacterial identification by angle resolved dark-field imaging.

Wilson, Benjamin K / Vigil, Genevieve D

Biomedical optics express

2013 Volume 4, Issue 9, Page(s) 1692–1701

Abstract: We propose and demonstrate a dark-field imaging technique capable of automated identification of individual bacteria. An 87-channel multispectral system capable of angular and spectral resolution was used to measure the scattering spectrum of various ... ...

Abstract	We propose and demonstrate a dark-field imaging technique capable of automated identification of individual bacteria. An 87-channel multispectral system capable of angular and spectral resolution was used to measure the scattering spectrum of various bacteria in culture smears. Spectra were compared between various species and between various preparations of the same species. A 15-channel system was then used to prove the viability of bacterial identification with a relatively simple microscope system. A simple classifier was able to identify four of six bacterial species with greater than 90% accuracy in bacteria-by-bacteria testing.
Language	English
Publishing date	2013-08-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.4.001692
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Article ; Online: Super-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy.

Zhang, Yide / Khan, Aamir A / Vigil, Genevieve D / Howard, Scott S

Optics express

2016 Volume 24, Issue 18, Page(s) 20862–20867

Abstract: We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the ... ...

Abstract	We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the theoretical limit of conventional MPM-FD-FLIM. Additionally, this technique produces unprecedented sensitivity over a large range of fluorescence lifetimes. These results are achieved through simple modifications to data analysis in a conventional MPM-FD-FLIM microscope and are based on an analytical model describing the signal-to-noise ratio (SNR) of a MPM-FD-FLIM system [J. Opt. Soc. Am. A33, B1 (2016)]. Here we experimentally validate this model.
Language	English
Publishing date	2016-09-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.24.020862
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Article ; Online: Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy.

Zhang, Yide / Khan, Aamir A / Vigil, Genevieve D / Howard, Scott S

Journal of the Optical Society of America. A, Optics, image science, and vision

2016 Volume 33, Issue 7, Page(s) B1–B11

Abstract: Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is ... ...

Abstract	Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is fundamentally limited by the shot noise and fluorescence saturation, has not been quantitatively studied yet. In this paper, we investigate the SNR performance of the frequency-domain (FD) MPM-FLIM with two figures of merit: the photon economy in the limit of shot noise, and the normalized SNR in the limit of saturation. The theoretical results and Monte Carlo simulations find that two-photon FD-FLIM requires 50% fewer photons to achieve the same SNR as conventional one-photon FLIM. We also analytically show that the MPM-FD-FLIM can exploit the DC and higher harmonic components generated by nonlinear optical mixing of the excitation light to improve SNR, reducing the required number of photons by an additional 50%. Finally, the effect of fluorophore saturation on the experimental SNR performance is discussed.
Language	English
Publishing date	2016-07-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	283633-6
ISSN	1520-8532 ; 1084-7529 ; 0740-3232
ISSN (online)	1520-8532
ISSN	1084-7529 ; 0740-3232
DOI	10.1364/JOSAA.33.0000B1
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Article: Super-resolution fluorescence microscopy by stepwise optical saturation.

Zhang, Yide / Nallathamby, Prakash D / Vigil, Genevieve D / Khan, Aamir A / Mason, Devon E / Boerckel, Joel D / Roeder, Ryan K / Howard, Scott S

Biomedical optics express

2018 Volume 9, Issue 4, Page(s) 1613–1629

Abstract: Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy is ... ...

Abstract	Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy is not feasible in many applications. In this paper, we propose and demonstrate a saturation-based super-resolution fluorescence microscopy technique that can be easily implemented and requires neither additional hardware nor complex post-processing. The method is based on the principle of stepwise optical saturation (SOS), where
Language	English
Publishing date	2018-03-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.9.001613
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Article ; Online: Saturation-compensated measurements for fluorescence lifetime imaging microscopy.

Zhang, Yide / Vigil, Genevieve D / Cao, Lina / Khan, Aamir A / Benirschke, David / Ahmed, Tahsin / Fay, Patrick / Howard, Scott S

Optics letters

2017 Volume 42, Issue 1, Page(s) 155–158

Abstract: Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM ... ...

Abstract	Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM at high excitation powers. In this Letter, we present an analytical theoretical description of this error and present a method for compensating for this error in order to extract correct lifetime measurements in the limit of fluorophore saturation. We perform a series of simulations and experiments to validate our methods. The simulations and experiments show a 13.2× and a 2.6× increase in excitation range, respectively. The presented method is based on algorithms that can be easily applied to existing FLIM setups.
Language	English
Publishing date	2017-01-01
Publishing country	United States
Document type	Journal Article
ISSN	1539-4794
ISSN (online)	1539-4794
DOI	10.1364/OL.42.000155
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Article: Poor Sleep Quality Increases Gestational Weight Gain Rate in Pregnant People: Findings from the MADRES Study.

Bastain, Theresa / Naya, Christine / Yang, Tingyu / Vigil, Mario / Chen, Cindy / Chavez, Thomas / Toledo-Corral, Claudia / Farzan, Shohreh / Habre, Rima / Lerner, Deborah / Lurvey, Nathana / Grubbs, Brendan / Dunton, Genevieve / Breton, Carrie / Eckel, Sandrah

Research square

2023

Abstract: Background: Poor sleep quality is associated with weight gain in non-pregnant populations, but evidence in pregnant people is lacking. Our study examined the association between early-to-mid pregnancy sleep quality and weekly gestational weight gain ( ... ...

Abstract	Background: Poor sleep quality is associated with weight gain in non-pregnant populations, but evidence in pregnant people is lacking. Our study examined the association between early-to-mid pregnancy sleep quality and weekly gestational weight gain (GWG) rate during mid-to-late pregnancy by pre-pregnancy body mass index (BMI). Method: Participants were 316 pregnant participants from the Maternal and Developmental Risks from Environmental and Social Stressors (MADRES) study. During early-to-mid pregnancy, participants reported their sleep quality which was used to construct four categories: very poor, poor, good, and very good. Linear growth curve models examined the association between early-to-mid pregnancy sleep quality and weekly rate of GWG (kg/week) during mid-to-late pregnancy (> 20 weeks gestation), with a three-way cross-level interaction between gestational age, sleep quality, and pre-pregnancy BMI category. Models adjusted for ethnicity by birthplace, hypertensive disorders, perceived stress score, and physical activity level. Results: Overall, poorer early-to-mid pregnancy sleep quality was associated with increased weekly weight gain during mid-to-late pregnancy. For example, amongst normal weight participants, mid-to-late pregnancy weight gain was, on average, 0.39 kg (95% CI: 0.29, 0.48) per week for those with very good sleep quality, 0.53 kg (95% CI: 0.44, 0.61) per week for those with poor sleep quality, and 0.54 kg (95% CI: 0.46, 0.62) per week for those with very poor sleep quality during early-to-mid pregnancy. This difference in GWG rate was statistically significantly comparing very good to poor sleep (0.14 kg/week, 95% CI: 0.01, 0.26) and very good to very poor sleep (0.15kg/week, 85% CI: 0.02, 0.27). This association between sleep quality and GWG rate did not statistically differ by pre-pregnancy BMI. Conclusion: Our study found very poor early-to-mid pregnancy sleep quality was associated with higher mid-to-late pregnancy GWG rate. Incorporating pregnancy-specific sleep recommendations into routine obstetric care may be a critical next step in promoting healthy GWG.
Language	English
Publishing date	2023-09-25
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-2944456/v1
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Article ; Online: Mapping solar magnetic fields from the photosphere to the base of the corona.

Ishikawa, Ryohko / Bueno, Javier Trujillo / Del Pino Alemán, Tanausú / Okamoto, Takenori J / McKenzie, David E / Auchère, Frédéric / Kano, Ryouhei / Song, Donguk / Yoshida, Masaki / Rachmeler, Laurel A / Kobayashi, Ken / Hara, Hirohisa / Kubo, Masahito / Narukage, Noriyuki / Sakao, Taro / Shimizu, Toshifumi / Suematsu, Yoshinori / Bethge, Christian / De Pontieu, Bart /

Dalda, Alberto Sainz / Vigil, Genevieve D / Winebarger, Amy / Ballester, Ernest Alsina / Belluzzi, Luca / Štěpán, Jiří / Ramos, Andrés Asensio / Carlsson, Mats / Leenaarts, Jorrit

Science advances

2021 Volume 7, Issue 8

Abstract: Routine ultraviolet imaging of the Sun's upper atmosphere shows the spectacular manifestation of solar activity; yet, we remain blind to its main driver, the magnetic field. Here, we report unprecedented spectropolarimetric observations of an active ... ...

Abstract	Routine ultraviolet imaging of the Sun's upper atmosphere shows the spectacular manifestation of solar activity; yet, we remain blind to its main driver, the magnetic field. Here, we report unprecedented spectropolarimetric observations of an active region plage and its surrounding enhanced network, showing circular polarization in ultraviolet (Mg ii
Language	English
Publishing date	2021-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.abe8406
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Article ; Online: Skeletal cell YAP and TAZ combinatorially promote bone development.

Kegelman, Christopher D / Mason, Devon E / Dawahare, James H / Horan, Daniel J / Vigil, Genevieve D / Howard, Scott S / Robling, Alexander G / Bellido, Teresita M / Boerckel, Joel D

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2018 Volume 32, Issue 5, Page(s) 2706–2721

Abstract: ... osteoclastic remodeling.-Kegelman, C. D., Mason, D. E., Dawahare, J. H., Horan, D. J., Vigil, G. D., Howard, S ... S., Robling, A. G., Bellido, T. M., Boerckel, J. D. Skeletal cell YAP and TAZ combinatorially ...

Abstract	The functions of the paralogous transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) in bone are controversial. Each has been observed to promote or inhibit osteogenesis in vitro, with reports of both equivalent and divergent functions. Their combinatorial roles in bone physiology are unknown. We report that combinatorial YAP/TAZ deletion from skeletal lineage cells, using Osterix-Cre, caused an osteogenesis imperfecta-like phenotype with severity dependent on allele dose and greater phenotypic expressivity with homozygous TAZ vs. YAP ablation. YAP/TAZ deletion decreased bone accrual and reduced intrinsic bone material properties through impaired collagen content and organization. These structural and material defects produced spontaneous fractures, particularly in mice with homozygous TAZ deletion and caused neonatal lethality in dual homozygous knockouts. At the cellular level in vivo, YAP/TAZ ablation reduced osteoblast activity and increased osteoclast activity, in an allele dose-dependent manner, impairing bone accrual and remodeling. Transcriptionally, YAP/TAZ deletion and small-molecule inhibition of YAP/TAZ interaction with the transcriptional coeffector TEAD reduced osteogenic and collagen-related gene expression, both in vivo and in vitro. These data demonstrate that YAP and TAZ combinatorially promote bone development through regulation of osteoblast activity, matrix quality, and osteoclastic remodeling.-Kegelman, C. D., Mason, D. E., Dawahare, J. H., Horan, D. J., Vigil, G. D., Howard, S. S., Robling, A. G., Bellido, T. M., Boerckel, J. D. Skeletal cell YAP and TAZ combinatorially promote bone development.
MeSH term(s)	Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/metabolism ; Animals ; Bone Development ; Bone Matrix/metabolism ; Bone Remodeling ; Cell Cycle Proteins ; Gene Deletion ; Mice ; Mice, Knockout ; Osteoblasts/metabolism ; Osteoclasts/metabolism ; Osteogenesis Imperfecta/genetics ; Osteogenesis Imperfecta/metabolism ; Osteogenesis Imperfecta/pathology ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; Trans-Activators
Chemical Substances	Adaptor Proteins, Signal Transducing ; Cell Cycle Proteins ; Phosphoproteins ; Trans-Activators ; Wwtr1 protein, mouse ; Yap1 protein, mouse
Language	English
Publishing date	2018-01-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.201700872R
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Article ; Online: Increased Risk of Gestational Hypertension by Periconceptional Exposure to Ambient Air Pollution and Effect Modification by Prenatal Depression.

Niu, Zhongzheng / Habre, Rima / Yang, Tingyu / Chen, Xinci / Vigil, Mario / Barragan, Karina / Lurmann, Fred / Pavlovic, Nathan R / Grubbs, Brendan H / Toledo-Corral, Claudia M / Johnston, Jill / Dunton, Genevieve F / Lerner, Deborah / Lurvey, Nathana / Al-Marayati, Laila / Eckel, Sandrah P / Breton, Carrie V / Bastain, Theresa M / Farzan, Shohreh F

Hypertension (Dallas, Tex. : 1979)

2024

Abstract: Background: Air pollution has been associated with gestational hypertension (GH) and preeclampsia, but susceptible windows of exposure and potential vulnerability by comorbidities, such as prenatal depression, remain unclear.: Methods: We ascertained ...

Abstract	Background: Air pollution has been associated with gestational hypertension (GH) and preeclampsia, but susceptible windows of exposure and potential vulnerability by comorbidities, such as prenatal depression, remain unclear. Methods: We ascertained GH and preeclampsia cases in a prospective pregnancy cohort in Los Angeles, CA. Daily levels of ambient particles (with a diameter of ≤10 μm [PM Results: Among 619 participants, 60 developed preeclampsia and 42 developed GH. We identified a susceptible window for exposure to PM Conclusions: We identified periconception through early pregnancy as a susceptible window of air pollution exposure with an increased risk of GH. Prenatal depression increases vulnerability to air pollution exposure and GH.
Language	English
Publishing date	2024-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	423736-5
ISSN	1524-4563 ; 0194-911X ; 0362-4323
ISSN (online)	1524-4563
ISSN	0194-911X ; 0362-4323
DOI	10.1161/HYPERTENSIONAHA.123.22272
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