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  1. Article: Actinomycetes associated with hymenopteran insects: a promising source of bioactive natural products.

    Diarra, Umar / Osborne-Naikatini, Tamara / Subramani, Ramesh

    Frontiers in microbiology

    2024  Volume 15, Page(s) 1303010

    Abstract: In recent years, the insect microbiome has become the focus of many actinomycete researchers in their search for novel bioactive compounds with members of the order Hymenoptera at the forefront of the revolution. Hymenoptera encompasses all bees, wasps, ... ...

    Abstract In recent years, the insect microbiome has become the focus of many actinomycete researchers in their search for novel bioactive compounds with members of the order Hymenoptera at the forefront of the revolution. Hymenoptera encompasses all bees, wasps, ants, and sawflies and is the third largest insect order by species richness. Additionally, Hymenoptera is the most diverse insect order in terms of ecological roles, behaviors, and social systems, thus making it an ideal starting point in the search for symbiotic actinomycetes. The aim of this review is to summarize current knowledge on hymenopteran associations with actinomycetes including information on interactions between actinomycetes and hymenopterans, isolation, and screening methodologies, as well as novel actinomycete species and natural products discovered between early 2013 and 2023. A total of 19 new species were discovered within this time period, with the genus
    Language English
    Publishing date 2024-02-28
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2024.1303010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Improving therapeutic potential in breast cancer via histone deacetylase inhibitor loaded nanofibrils.

    Senthilkumar, Praveetha / Gogoi, Bhaskar / Dhan, Swati Smita / Subramani, Ramesh / Pushparaj, Charumathi / Mahesh, Ayyavu

    Drug development research

    2024  Volume 85, Issue 2, Page(s) e22172

    Abstract: Epigenetic modifications play a significant role in cancer progression, making them potential targets for therapy. Histone deacetylase inhibitors have shown promise in inhibiting cancer cell growth, including in breast cancer (BC). In this research, we ... ...

    Abstract Epigenetic modifications play a significant role in cancer progression, making them potential targets for therapy. Histone deacetylase inhibitors have shown promise in inhibiting cancer cell growth, including in breast cancer (BC). In this research, we examined the potential of using suberoyl anilide hydroxamic acid (SAHA)-loaded β-lg nanofibrils as a drug delivery system for triple-negative BC cell lines. We assessed their impact on cell cycle progression, apoptosis, levels of reactive oxygen species, and mitochondrial membrane potential in cancer cells. The combination of SAHA and β-lg nanofibrils demonstrated enhanced efficacy in inhibiting cell growth, inducing cell cycle arrest, and promoting apoptosis (43.78%) compared to SAHA alone (40.09%). Moreover, it effectively targeted cancer cells without promoting drug resistance while using a low concentration of the nanofibrils. These findings underscore the promising potential of nanofibril-based drug delivery systems for BC treatment.
    MeSH term(s) Humans ; Female ; Histone Deacetylase Inhibitors/pharmacology ; Breast Neoplasms/drug therapy ; Breast Neoplasms/metabolism ; Hydroxamic Acids/pharmacology ; Vorinostat/pharmacology ; Vorinostat/therapeutic use ; Cell Cycle ; Apoptosis ; Cell Proliferation ; Cell Line, Tumor ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use
    Chemical Substances Histone Deacetylase Inhibitors ; Hydroxamic Acids ; Vorinostat (58IFB293JI) ; Antineoplastic Agents
    Language English
    Publishing date 2024-03-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604587-x
    ISSN 1098-2299 ; 0272-4391
    ISSN (online) 1098-2299
    ISSN 0272-4391
    DOI 10.1002/ddr.22172
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Microbiological analysis, antimicrobial activity, heavy-metals content and physico-chemical properties of Fijian mud pool samples.

    Pipite, Atanas / Siro, Galana / Subramani, Ramesh / Srinivasan, Sathiyaraj

    The Science of the total environment

    2022  Volume 854, Page(s) 158725

    Abstract: The hot springs are home to a rich bacterial diversity which could be the source of enzymes, antibiotics and many other commercially important products. Most of the hot springs present in Fiji are unexplored and their analysis of microbial diversity ... ...

    Abstract The hot springs are home to a rich bacterial diversity which could be the source of enzymes, antibiotics and many other commercially important products. Most of the hot springs present in Fiji are unexplored and their analysis of microbial diversity could be of great interest in facilitating various industrial, agricultural and medicinal applications. This study is an attempt to evaluate the heavy metal concentration and to analyze the comprehensive bacterial diversity of two Fijian thermal mud pools, namely Sabeto and Tifajek. The two hot springs have a pH of 7.28 to 7.19 and a temperature of 32.2 to 38.8 °C, respectively. Mean metal concentrations of the studied mud samples ranged from 4.758 to 6.870 mg/kg and followed a decreasing sequence as Fe > Mn > Zn > Na > Ni > Cd > Ca > Cr > Cu. Levels of Fe, Na, Mn, Zn, Ni, Cd, Ca, Cr, Cu in the mud pool samples were within World Health Organisation (WHO) limits, while Cd was above regulatory limits. The heavy metals analysis results showed that both mud pools had high values for Cd, above the WHO limit of 3 mg/kg. In addition, 8 strains of actinomycetes were successfully identified for the first time in the Sabeto mud pool, where most of them showed antibacterial activity. The genetic identification of most isolates was determined in BLASTn analyses of their 16S rRNA sequences. Isolates were identified as that of Streptomyces, Nocardia and Rhodococcus genus. Further, AntiSMASH results of the closest relatives of cultured actinobacteria have shown to produce antibiotics, natural pesticides and other compounds of various usage. This study also found no fecal coliforms and supports existing knowledge and practice of using Fijian thermal mud pools for their therapeutic properties. Overall, the presented work indicated that the studied mud pools have therapeutic properties, harboring wealth of bacteria with antibiotic profiles and were risk free from health-related issues of heavy metals and disease-causing pathogens. It provides great insight into the studied mud pools which serves as a baseline from which further heavy metal monitoring or mitigation programs and microbial researches can be conducted.
    MeSH term(s) Environmental Monitoring/methods ; RNA, Ribosomal, 16S ; Cadmium/analysis ; Metals, Heavy/analysis ; Bacteria ; Anti-Bacterial Agents/analysis ; Risk Assessment
    Chemical Substances RNA, Ribosomal, 16S ; Cadmium (00BH33GNGH) ; Metals, Heavy ; Anti-Bacterial Agents
    Language English
    Publishing date 2022-09-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2022.158725
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microbiological analysis, antimicrobial activity, heavy-metals content and physico-chemical properties of Fijian mud pool samples

    Pipite, Atanas / Siro, Galana / Subramani, Ramesh / Srinivasan, Sathiyaraj

    Science of the Total Environment. 2023 Jan., v. 854 p.158725-

    2023  

    Abstract: The hot springs are home to a rich bacterial diversity which could be the source of enzymes, antibiotics and many other commercially important products. Most of the hot springs present in Fiji are unexplored and their analysis of microbial diversity ... ...

    Abstract The hot springs are home to a rich bacterial diversity which could be the source of enzymes, antibiotics and many other commercially important products. Most of the hot springs present in Fiji are unexplored and their analysis of microbial diversity could be of great interest in facilitating various industrial, agricultural and medicinal applications. This study is an attempt to evaluate the heavy metal concentration and to analyze the comprehensive bacterial diversity of two Fijian thermal mud pools, namely Sabeto and Tifajek. The two hot springs have a pH of 7.28 to 7.19 and a temperature of 32.2 to 38.8 °C, respectively. Mean metal concentrations of the studied mud samples ranged from 4.758 to 6.870 mg/kg and followed a decreasing sequence as Fe > Mn > Zn > Na > Ni > Cd > Ca > Cr > Cu. Levels of Fe, Na, Mn, Zn, Ni, Cd, Ca, Cr, Cu in the mud pool samples were within World Health Organisation (WHO) limits, while Cd was above regulatory limits. The heavy metals analysis results showed that both mud pools had high values for Cd, above the WHO limit of 3 mg/kg. In addition, 8 strains of actinomycetes were successfully identified for the first time in the Sabeto mud pool, where most of them showed antibacterial activity. The genetic identification of most isolates was determined in BLASTn analyses of their 16S rRNA sequences. Isolates were identified as that of Streptomyces, Nocardia and Rhodococcus genus. Further, AntiSMASH results of the closest relatives of cultured actinobacteria have shown to produce antibiotics, natural pesticides and other compounds of various usage. This study also found no fecal coliforms and supports existing knowledge and practice of using Fijian thermal mud pools for their therapeutic properties. Overall, the presented work indicated that the studied mud pools have therapeutic properties, harboring wealth of bacteria with antibiotic profiles and were risk free from health-related issues of heavy metals and disease-causing pathogens. It provides great insight into the studied mud pools which serves as a baseline from which further heavy metal monitoring or mitigation programs and microbial researches can be conducted.
    Keywords Nocardia ; Streptomyces ; World Health Organization ; antibacterial properties ; antibiotics ; environment ; genetic testing ; heavy metals ; pH ; risk ; temperature ; therapeutics ; Fiji ; Hot springs ; Mud pool ; Therapeutic properties ; Heavy metal ; Actinobacteria ; Antibacterial activity
    Language English
    Dates of publication 2023-01
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2022.158725
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Assessment of the Endotracheal Tube Tip Position by Bedside Ultrasound in a Pediatric Intensive Care Unit: A Cross-sectional Study.

    Subramani, Seenivasan / Parameswaran, Narayanan / Ananthkrishnan, Ramesh / Abraham, Shilpa / Chidambaram, Muthu / Rameshkumar, Ramachandran / Subramanian, Mahadevan

    Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine

    2023  Volume 26, Issue 11, Page(s) 1218–1224

    Abstract: ... 98.10%) but poor specificity (50.0%).: How to cite this article: Subramani S, Parameswaran N ...

    Abstract Introduction: The chest X-ray (CXR) is the standard of practice to assess the tip of the endotracheal tube (ETT) in ventilated children. In many hospitals, it takes hours to get a bedside CXR, and it has radiation exposure. The objective of this study was to find the utility of bedside ultrasound (USG), in assessing the ETT tip position in a Pediatric Intensive Care Unit (PICU).
    Methods: It was a prospective study conducted in the PICU of a tertiary care center involving 135 children aged from 1 month to 60 months, requiring endotracheal intubation. In this study, the authors compared the position of the ETT tip by the CXR (gold standard) and USG. The CXR was taken in children to assess the correct position of the tip of ETT. The USG was used to measure the distance between the tip of ETT and the arch of the aorta, thrice in the same patient. The mean of the three USG readings was compared with the distance between the tip of the ETT and carina in CXR.
    Results: The reliability of three USG readings was tested by absolute agreement coefficient in intraclass correlation (ICC), 0.986 (95% CI: 0.981-0.989). The sensitivity and specificity of the USG in identifying the correct position of the ETT tip in children when compared to CXR were 98.10% (95% CI: 93.297-99.71%) and 50.0% (95% CI: 31.30-68.70%), respectively.
    Conclusion: In ventilated children <60 months of age, identifying the tip of ETTs by bedside the USG has good sensitivity (98.10%) but poor specificity (50.0%).
    How to cite this article: Subramani S, Parameswaran N, Ananthkrishnan R, Abraham S, Chidambaram M, Rameshkumar R,
    Language English
    Publishing date 2023-02-25
    Publishing country India
    Document type Case Reports
    ZDB-ID 2121263-6
    ISSN 1998-359X ; 0972-5229
    ISSN (online) 1998-359X
    ISSN 0972-5229
    DOI 10.5005/jp-journals-10071-24355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites.

    Siro, Galana / Pipite, Atanas / Christi, Ketan / Srinivasan, Sathiyaraj / Subramani, Ramesh

    Microorganisms

    2022  Volume 10, Issue 7

    Abstract: Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have ...

    Abstract Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have a colossal genome with a wide array of genes that code for several bioactive metabolites and enzymes. Numerous studies have reported the isolation and screening of millions of strains of actinomycetes from various habitats for specialized metabolites worldwide. Looking at the extent of the importance of actinomycetes in various fields, corals are highlighted as a potential hotspot for untapped secondary metabolites and new bioactive metabolites. Unfortunately, knowledge about the diversity, distribution and biochemistry of marine actinomycetes compared to hard corals is limited. In this review, we aim to summarize the recent knowledge on the isolation, diversity, distribution and discovery of natural compounds from marine actinomycetes associated with hard corals. A total of 11 new species of actinomycetes, representing nine different families of actinomycetes, were recovered from hard corals during the period from 2007 to 2022. In addition, this study examined a total of 13 new compounds produced by five genera of actinomycetes reported from 2017 to 2022 with antibacterial, antifungal and cytotoxic activities. Coral-derived actinomycetes have different mechanisms of action against their competitors.
    Language English
    Publishing date 2022-07-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10071349
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products.

    Subramani, Ramesh / Sipkema, Detmer

    Marine drugs

    2019  Volume 17, Issue 5

    Abstract: Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and ... ...

    Abstract Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and novel bioactive compounds. In this review, we aim to summarize the present knowledge on the isolation, diversity, distribution and natural product discovery of marine rare actinomycetes reported from mid-2013 to 2017. A total of 97 new species, representing 9 novel genera and belonging to 27 families of marine rare actinomycetes have been reported, with the highest numbers of novel isolates from the families Pseudonocardiaceae, Demequinaceae, Micromonosporaceae and Nocardioidaceae. Additionally, this study reviewed 167 new bioactive compounds produced by 58 different rare actinomycete species representing 24 genera. Most of the compounds produced by the marine rare actinomycetes present antibacterial, antifungal, antiparasitic, anticancer or antimalarial activities. The highest numbers of natural products were derived from the genera
    MeSH term(s) Actinobacteria/chemistry ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/isolation & purification ; Anti-Bacterial Agents/pharmacology ; Antifungal Agents/chemistry ; Antifungal Agents/isolation & purification ; Antifungal Agents/pharmacology ; Antimalarials/chemistry ; Antimalarials/isolation & purification ; Antimalarials/pharmacology ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/isolation & purification ; Antineoplastic Agents/pharmacology ; Antiparasitic Agents/chemistry ; Antiparasitic Agents/isolation & purification ; Antiparasitic Agents/pharmacology ; Aquatic Organisms/chemistry ; Biological Products/chemistry ; Biological Products/isolation & purification ; Biological Products/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Antifungal Agents ; Antimalarials ; Antineoplastic Agents ; Antiparasitic Agents ; Biological Products
    Language English
    Publishing date 2019-04-26
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md17050249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Marine Actinomycetes Associated with Stony Corals

    Galana Siro / Atanas Pipite / Ketan Christi / Sathiyaraj Srinivasan / Ramesh Subramani

    Microorganisms, Vol 10, Iss 7, p

    A Potential Hotspot for Specialized Metabolites

    2022  Volume 1349

    Abstract: Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have ...

    Abstract Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have a colossal genome with a wide array of genes that code for several bioactive metabolites and enzymes. Numerous studies have reported the isolation and screening of millions of strains of actinomycetes from various habitats for specialized metabolites worldwide. Looking at the extent of the importance of actinomycetes in various fields, corals are highlighted as a potential hotspot for untapped secondary metabolites and new bioactive metabolites. Unfortunately, knowledge about the diversity, distribution and biochemistry of marine actinomycetes compared to hard corals is limited. In this review, we aim to summarize the recent knowledge on the isolation, diversity, distribution and discovery of natural compounds from marine actinomycetes associated with hard corals. A total of 11 new species of actinomycetes, representing nine different families of actinomycetes, were recovered from hard corals during the period from 2007 to 2022. In addition, this study examined a total of 13 new compounds produced by five genera of actinomycetes reported from 2017 to 2022 with antibacterial, antifungal and cytotoxic activities. Coral-derived actinomycetes have different mechanisms of action against their competitors.
    Keywords marine actinomycetes ; actinobacteria ; microbial secondary metabolite ; scleractinian coral ; antimicrobial resistance ; Biology (General) ; QH301-705.5
    Subject code 500
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: Development of Almond gum/alginate composites to enhance the shelf-life of post-harvest Solanum Lycopersicum L

    Suresh, Siva Nandhini / Puspharaj, Charumathi / Subramani, Ramesh

    Food hydrocolloids for health. 2022 Dec., v. 2

    2022  

    Abstract: Almond Gum/alginate composite (AG/AL) composites namely A1, A2 and A3 were prepared and their physicochemical properties were evaluated. The measured film thickness, moisture and solubility were indistinguishable among the films whereas water contact ... ...

    Abstract Almond Gum/alginate composite (AG/AL) composites namely A1, A2 and A3 were prepared and their physicochemical properties were evaluated. The measured film thickness, moisture and solubility were indistinguishable among the films whereas water contact angle analysis shows that A2 (83.8±1.65) and A3 (86.2±3.19) film has high hydrophobicity than A1 (58.4±3.21). By increasing plasticizer concentration from 0.4% to 0.66%, elongation was increased from 30 to 53 % whereas tensile strength (3.16 to 1.90 Mpa) and water vapor transmission rate (WVTR) decreased from 212 to 160 g/m²/24hr. FT-IR analysis shows that the edible film has O-H, C-H, C=O and –COO functional groups and SEM analysis reveals that the prepared film has even surface due to the strong interaction between polymer and plasticizer. TGA results show major weight loss at 100°C, 190-220°C and 350°C-400°C due to the dehydration/decomposition of water molecules and carbon containing functional groups. Film solution was used as an edible coating in tomatoes and weight loss (WL), titratable acidity (TA), Total soluble solids (TSS), ascorbic acid content (AAC) and anti-microbial activity were determined. The WL analysis shows a significant delay in decay of coated tomatoes over 40 days of storage time whereas control tomatoes decayed on day 25. TA and TSS test confirms that the coating can significantly (P<0.05) prevent the acidity level of tomaotes. Furthermore, the AG/AL coating has great activity against E. coli, Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus. Therefore, the present study confirms that AG/AL has superior properties and potential as edible films or coating.
    Keywords Bacillus subtilis ; Enterococcus faecalis ; Escherichia coli ; Solanum lycopersicum ; Staphylococcus aureus ; alginates ; almonds ; antimicrobial properties ; ascorbic acid ; carbon ; contact angle ; edible films ; hydrocolloids ; hydrophobicity ; plasticizers ; polymers ; shelf life ; solubility ; storage time ; tensile strength ; titratable acidity ; water vapor ; weight loss
    Language English
    Dates of publication 2022-12
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 2667-0259
    DOI 10.1016/j.fhfh.2022.100087
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products

    Subramani, Ramesh / Sipkema, Detmer

    Marine drugs. 2019 Apr. 26, v. 17, no. 5

    2019  

    Abstract: Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and ... ...

    Abstract Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and novel bioactive compounds. In this review, we aim to summarize the present knowledge on the isolation, diversity, distribution and natural product discovery of marine rare actinomycetes reported from mid-2013 to 2017. A total of 97 new species, representing 9 novel genera and belonging to 27 families of marine rare actinomycetes have been reported, with the highest numbers of novel isolates from the families Pseudonocardiaceae, Demequinaceae, Micromonosporaceae and Nocardioidaceae. Additionally, this study reviewed 167 new bioactive compounds produced by 58 different rare actinomycete species representing 24 genera. Most of the compounds produced by the marine rare actinomycetes present antibacterial, antifungal, antiparasitic, anticancer or antimalarial activities. The highest numbers of natural products were derived from the genera Nocardiopsis, Micromonospora, Salinispora and Pseudonocardia. Members of the genus Micromonospora were revealed to be the richest source of chemically diverse and unique bioactive natural products.
    Keywords Micromonospora ; Nocardioidaceae ; Nocardiopsis ; Pseudonocardia ; bioactive compounds ; marine environment ; new species ; secondary metabolites
    Language English
    Dates of publication 2019-0426
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2175190-0
    ISSN 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md17050249
    Database NAL-Catalogue (AGRICOLA)

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