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Article ; Online: A Novel Fibrin Matrix Derived from Platelet-Rich Plasma: Protocol and Characterization.

Delgado, Diego / Beitia, Maider / Mercader Ruiz, Jon / Sánchez, Pello / Montoya-Alzola, Marta / Fiz, Nicolás / Sánchez, Mikel

International journal of molecular sciences

2024 Volume 25, Issue 7

Abstract: Although fibrin matrices derived from Platelet-Rich Plasma (PRP) are widely used in regenerative medicine, they have some limitations that can hinder their application. Modifying the composition of the PRP-derived fibrin matrix may improve its properties, ...

Abstract	Although fibrin matrices derived from Platelet-Rich Plasma (PRP) are widely used in regenerative medicine, they have some limitations that can hinder their application. Modifying the composition of the PRP-derived fibrin matrix may improve its properties, making it suitable for certain medical uses. Three types of fibrin matrices were obtained: a PRP-derived fibrin matrix (FM), a PRP-derived fibrin matrix with a high fibrinogen content and platelets (FM-HFP) and a PRP-derived fibrin matrix with a high fibrinogen content (FM-HF). The fibrinogen levels, biomechanical properties and cell behavior were analyzed. The presence of platelets in the FM-HFP generated an inconsistent fibrin matrix that was discarded for the rest of the analysis. The fibrinogen levels in the FM-FH were higher than those in the FM (
MeSH term(s)	Platelet-Rich Plasma ; Blood Coagulation ; Blood Platelets ; Fibrin ; Fibrinogen
Chemical Substances	Fibrin (9001-31-4) ; Fibrinogen (9001-32-5)
Language	English
Publishing date	2024-04-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25074069
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Action of Platelet-Rich Plasma on In Vitro Cellular Bioactivity: More than Platelets.

Beitia, Maider / Delgado, Diego / Mercader, Jon / Sánchez, Pello / López de Dicastillo, Leonor / Sánchez, Mikel

International journal of molecular sciences

2023 Volume 24, Issue 6

Abstract: Platelet-rich plasma (PRP) is a biological therapy in which one of the mechanisms of action is the stimulation of biological processes such as cell proliferation. The size of PRP's effect depends on multiple factors, one of the most important being the ... ...

Abstract	Platelet-rich plasma (PRP) is a biological therapy in which one of the mechanisms of action is the stimulation of biological processes such as cell proliferation. The size of PRP's effect depends on multiple factors, one of the most important being the composition of PRP. The aim of this study was to analyze the relationship between cell proliferation and the levels of certain growth factors (IGF-1, HGF, PDGF, TGF-β and VEG) in PRP. First, the composition and effect on cell proliferation of PRP versus platelet-poor plasma (PPP) were compared. Subsequently, the correlation between each growth factor of PRP and cell proliferation was evaluated. Cell proliferation was higher in cells incubated with lysates derived from PRP compared to those cultured with lysates derived from PPP. In terms of composition, the levels of PDGF, TGF-β, and VEGF were significantly higher in PRP. When analyzing the PRP growth factors, IGF-1 was the only factor that correlated significantly with cell proliferation. Of those analyzed, the level of IGF-1 was the only one that did not correlate with platelet levels. The magnitude of PRP's effect depends not only on platelet count but also on other platelet-independent molecules.
MeSH term(s)	Platelet-Derived Growth Factor/metabolism ; Insulin-Like Growth Factor I/metabolism ; Blood Platelets/metabolism ; Transforming Growth Factor beta/metabolism ; Platelet-Rich Plasma/metabolism
Chemical Substances	Platelet-Derived Growth Factor ; Insulin-Like Growth Factor I (67763-96-6) ; Transforming Growth Factor beta
Language	English
Publishing date	2023-03-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24065367
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Article ; Online: Action of Platelet-Rich Plasma on In Vitro Cellular Bioactivity

Maider Beitia / Diego Delgado / Jon Mercader / Pello Sánchez / Leonor López de Dicastillo / Mikel Sánchez

International Journal of Molecular Sciences, Vol 24, Iss 5367, p

More than Platelets

2023 Volume 5367

Abstract: Platelet-rich plasma (PRP) is a biological therapy in which one of the mechanisms of action is the stimulation of biological processes such as cell proliferation. The size of PRP’s effect depends on multiple factors, one of the most important being the ... ...

Abstract	Platelet-rich plasma (PRP) is a biological therapy in which one of the mechanisms of action is the stimulation of biological processes such as cell proliferation. The size of PRP’s effect depends on multiple factors, one of the most important being the composition of PRP. The aim of this study was to analyze the relationship between cell proliferation and the levels of certain growth factors (IGF-1, HGF, PDGF, TGF-β and VEG) in PRP. First, the composition and effect on cell proliferation of PRP versus platelet-poor plasma (PPP) were compared. Subsequently, the correlation between each growth factor of PRP and cell proliferation was evaluated. Cell proliferation was higher in cells incubated with lysates derived from PRP compared to those cultured with lysates derived from PPP. In terms of composition, the levels of PDGF, TGF-β, and VEGF were significantly higher in PRP. When analyzing the PRP growth factors, IGF-1 was the only factor that correlated significantly with cell proliferation. Of those analyzed, the level of IGF-1 was the only one that did not correlate with platelet levels. The magnitude of PRP’s effect depends not only on platelet count but also on other platelet-independent molecules.
Keywords	platelet-rich plasma ; growth factors ; platelets ; insulin-like growth factor-1 ; IGF-1 ; cell proliferation ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	630
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Method to obtain a plasma rich in platelet- and plasma-growth factors based on water evaporation.

Mercader Ruiz, Jon / Beitia, Maider / Delgado, Diego / Sánchez, Pello / Sánchez, Miren Begoña / Oraa, Jaime / Benito-Lopez, Fernando / Basabe-Desmonts, Lourdes / Sánchez, Mikel

PloS one

2024 Volume 19, Issue 2, Page(s) e0297001

Abstract: Platelet-Rich Plasma, also known as PRP, is an autologous biologic product used in medicine as a treatment for tissue repair. Nowadays, the majority of PRP obtention methods enrich only platelets, not considering extraplatelet biomolecules, which take ... ...

Abstract	Platelet-Rich Plasma, also known as PRP, is an autologous biologic product used in medicine as a treatment for tissue repair. Nowadays, the majority of PRP obtention methods enrich only platelets, not considering extraplatelet biomolecules, which take part in several cell processes. In the present work, a novel PRP preparation method was developed to obtain a PRP rich in both platelet and plasma extraplatelet molecules. The method is based on the evaporation of the water of the plasma using a rotary evaporator. With this new methodology an increase in plasmatic growth factors and, as a consequence, a better dermal fibroblast cell viability was achieved, compared to a standard PRP formulation. This novel PRP product obtained with this new methodology showed promising results in vitro as an improved PRP treatment in future application.
MeSH term(s)	Intercellular Signaling Peptides and Proteins/pharmacology ; Platelet-Rich Plasma ; Blood Platelets ; Wound Healing
Chemical Substances	Intercellular Signaling Peptides and Proteins
Language	English
Publishing date	2024-02-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0297001
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Article ; Online: The effect of short-term cryopreservation on the properties and functionality of platelet-rich plasma.

Beitia, Maider / Delgado, Diego / Mercader, Jon / Gimeno, Irene / Espregueira-Mendes, João / Aizpurua, Beatriz / Sánchez, Mikel

Platelets

2023 Volume 34, Issue 1, Page(s) 2210243

Abstract: Platelet Rich Plasma (PRP) is a biological treatment which, thanks to its enhanced growth factors content, is widely used in the field of regenerative medicine for its reparative effects. Although it is usually used fresh immediately after preparation, ... ...

Abstract	Platelet Rich Plasma (PRP) is a biological treatment which, thanks to its enhanced growth factors content, is widely used in the field of regenerative medicine for its reparative effects. Although it is usually used fresh immediately after preparation, its freezing for preservation for future usage could be key in increasing its versatility and new applications. To assess the suitability of freezing, after collecting PRP and platelet lysates (PL) from 6 patients, they were preserved for 1 or 3 months at temperatures of -20ºC and -80°C. Measurements were then made on platelet number and integrity, growth factor levels, biomechanical properties of the clot and its bioactivity on cultured cells. Fresh PRP and PL were used as controls. The results showed an increase in platelet size (
MeSH term(s)	Humans ; Cryopreservation ; Intercellular Signaling Peptides and Proteins/metabolism ; Cells, Cultured ; Cell Culture Techniques ; Platelet-Rich Plasma/metabolism
Chemical Substances	Intercellular Signaling Peptides and Proteins
Language	English
Publishing date	2023-05-11
Publishing country	England
Document type	Journal Article
ZDB-ID	1034283-7
ISSN	1369-1635 ; 0953-7104
ISSN (online)	1369-1635
ISSN	0953-7104
DOI	10.1080/09537104.2023.2210243
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Article ; Online: The role of sociability in social instability stress: Behavioral, neuroendocrine and monoaminergic effects.

Díez-Solinska, Alina / Azkona, Garikoitz / Muñoz-Culla, Maider / Beitia-Oyarzabal, Garikoitz / Goñi-Balentziaga, Olatz / Gómez-Lazaro, Eneritz / Vegas, Oscar

Physiology & behavior

2023 Volume 270, Page(s) 114306

Abstract: Extensive literature has reported a link between social stress and mental health. In this complex relationship, individual strategies for coping with social stress are thought to have a possible modulating effect, with sociability being a key factor. ... ...

Abstract	Extensive literature has reported a link between social stress and mental health. In this complex relationship, individual strategies for coping with social stress are thought to have a possible modulating effect, with sociability being a key factor. Despite the higher incidence of affective disorders in females and sex-related neurochemical differences, female populations have been understudied. The aim of the present study was, therefore, to analyze the behavioral, neuroendocrine, and neurochemical effects of stress in female OF1 mice, paying special attention to social connectedness (female mice with high vs low sociability). To this end, subjects were exposed to the Chronic Social Instability Stress (CSIS) model for four weeks. Although female mice exposed to CSIS had increased arousal, there was no evidence of depressive-like behavior. Neither did exposure to CSIS affect corticosterone levels, although it did increase the MR/GR ratio by decreasing GR expression. Female mice exposed to CSIS had higher noradrenaline and dopamine levels in the hippocampus and striatum respectively, with a lower monoaminergic turnover, resulting in an increased arousal. CSIS increased serotonin levels in both the hippocampus and striatum. Similarly, CSIS was found to reduce kynurenic acid, 3-HK, and IDO and iNOS enzyme levels in the hippocampus. Interestingly, the observed decrease in IDO synthesis and the increased serotonin and dopamine levels in the striatum were only found in subjects with high sociability. These highly sociable female mice also had significantly lower levels of noradrenaline in the striatum after CSIS application. Overall, our model has produced neuroendocrine and neurochemical but not behavioral changes, so it has not allowed us to study sociability in depth. Therefore, a model that induces both molecular and behavioral phenotypes should be applied to determine the role of sociability.
MeSH term(s)	Mice ; Female ; Animals ; Dopamine/metabolism ; Serotonin/metabolism ; Neurosecretory Systems/metabolism ; Hippocampus/metabolism ; Stress, Psychological/metabolism ; Norepinephrine/metabolism
Chemical Substances	Dopamine (VTD58H1Z2X) ; Serotonin (333DO1RDJY) ; Norepinephrine (X4W3ENH1CV)
Language	English
Publishing date	2023-07-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3907-x
ISSN	1873-507X ; 0031-9384
ISSN (online)	1873-507X
ISSN	0031-9384
DOI	10.1016/j.physbeh.2023.114306
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Article ; Online: Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization.

Saumell-Esnaola, Miquel / Delgado, Diego / García Del Caño, Gontzal / Beitia, Maider / Sallés, Joan / González-Burguera, Imanol / Sánchez, Pello / López de Jesús, Maider / Barrondo, Sergio / Sánchez, Mikel

International journal of molecular sciences

2022 Volume 23, Issue 5

Abstract: Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either in free form or encapsulated in exosomes, which ... ...

Abstract	Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either in free form or encapsulated in exosomes, which have been proven to promote tissue repair and regeneration. Translational research on the potential of exosomes as a safe nanosystem for therapeutic cargo delivery requires standardizing exosome isolation methods along with their molecular and morphological characterization. With this aim, we isolated and characterized the exosomes released by human PRP platelets. Western blot analysis revealed that CaCl
MeSH term(s)	Blood Platelets/metabolism ; Cytokines/metabolism ; Exosomes/metabolism ; Humans ; Platelet-Rich Plasma/metabolism ; Wound Healing
Chemical Substances	Cytokines
Language	English
Publishing date	2022-03-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23052861
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Article: Custom 3D-Printed Cutting Guides for Femoral Osteotomy in Rotational Malalignment Due to Diaphyseal Fractures: Surgical Technique and Case Series.

Oraa, Jaime / Beitia, Maider / Fiz, Nicolás / González, Sergio / Sánchez, Xabier / Delgado, Diego / Sánchez, Mikel

Journal of clinical medicine

2021 Volume 10, Issue 15

Abstract: Femoral shaft fractures are one of the most common injuries in trauma patients. The gold standard treatment consists of closed reduction and intramedullary nailing, providing a high fracture healing rate and allowing early mobilization. However, ... ...

Abstract	Femoral shaft fractures are one of the most common injuries in trauma patients. The gold standard treatment consists of closed reduction and intramedullary nailing, providing a high fracture healing rate and allowing early mobilization. However, rotational malalignment is a well-known complication following this procedure, and excessive femoral anteversion or femoral retroversion can trigger functional complaints. In order to achieve the ideal degree of femoral rotation, a 3D planning and printing cutting guides procedure was developed to correct femoral malrotation. A patient series with malalignment after a femoral diaphyseal fracture was operated on with the customized guides and evaluated in this study. Computed tomography scans were performed to accurately determine the number of degrees of malrotation, allowing the design of specific and personalized surgical guides to correct these accurately. Once designed, they were produced by 3D printing. After surgery with the customized guides to correct femoral malrotation, all patients presented a normalized anteversion angle of the femur (average -10.3°, range from -5° to -15°), according to their contralateral limb. These data suggest that the use of customized cutting guides for femoral osteotomy is a safe and reproducible surgical technique that offers precise results when correcting femoral malrotation.
Language	English
Publishing date	2021-07-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm10153366
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Article: Method Based on Ultrafiltration to Obtain a Plasma Rich in Platelet and Plasma Growth Factors.

Mercader Ruiz, Jon / Beitia, Maider / Delgado, Diego / Sánchez, Pello / Guadilla, Jorge / Pérez de Arrilucea, Cristina / Benito-Lopez, Fernando / Basabe-Desmonts, Lourdes / Sánchez, Mikel

Journal of clinical medicine

2023 Volume 12, Issue 18

Abstract: Platelet-Rich Plasma (PRP) is an autologous biological product which, due to its regenerative capacity, is currently used in different fields of medicine. This biological treatment has proven to be effective in numerous research studies due to its high ... ...

Abstract	Platelet-Rich Plasma (PRP) is an autologous biological product which, due to its regenerative capacity, is currently used in different fields of medicine. This biological treatment has proven to be effective in numerous research studies due to its high content of growth factors released by platelets. However, the current systems used to obtain PRP do not enrich the growth factors and cytokines outside platelets. Considering this, the present work aims to develop a new technique by which all the biomolecules present in plasma are enriched. Thus, a new method based on ultrafiltration has been developed for the obtaining of the novel PRP. By this method, ultrafiltration of the plasma water is carried out using a 3KDa filtering unit. The results showed that the technique was able to concentrate extraplatelet factors, such as IGF-1 and HGF, in contrast with conventional plasmas. Thus, the cultured cells responded with increased viability to this new PRP. These results could provide a new approach to the treatment of injuries requiring regenerative medicine, potentially improving the outcomes of the conventional PRPs.
Language	English
Publishing date	2023-09-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm12185941
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Article ; Online: Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma

Miquel Saumell-Esnaola / Diego Delgado / Gontzal García del Caño / Maider Beitia / Joan Sallés / Imanol González-Burguera / Pello Sánchez / Maider López de Jesús / Sergio Barrondo / Mikel Sánchez

International Journal of Molecular Sciences, Vol 23, Iss 2861, p

Biochemical and Morphological Characterization

2022 Volume 2861

Abstract	Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either in free form or encapsulated in exosomes, which have been proven to promote tissue repair and regeneration. Translational research on the potential of exosomes as a safe nanosystem for therapeutic cargo delivery requires standardizing exosome isolation methods along with their molecular and morphological characterization. With this aim, we isolated and characterized the exosomes released by human PRP platelets. Western blot analysis revealed that CaCl 2 -activated platelets (PLT-Exos-Ca 2+ ) released more exosomes than non-activated ones (PLT-Exos). Moreover, PLT-Exos-Ca 2+ exhibited a molecular signature that meets the most up-to-date biochemical criteria for platelet-derived exosomes and possessed morphological features typical of exosomes as assessed by transmission electron microscopy. Array analysis of 105 analytes including growth factors and cytokines showed that PLT-Exos-Ca 2+ exhibited lower levels of most analytes compared to PLT-Exos, but relatively higher levels of those consistently validated as components of the protein cargo of platelet exosomes. In summary, the present study provides new insights into the molecular composition of human platelet-derived exosomes and validates a method for isolating highly pure platelet exosomes as a basis for future preclinical studies in regenerative medicine and drug delivery.
Keywords	platelet-rich plasma ; human platelets ; platelet-derived-exosomes ; exosome markers ; cytokines ; growth factors ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	630
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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