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Article: Shiga Toxin-Producing and Enteroaggregative Escherichia coli in Animal, Foods, and Humans: Pathogenicity Mechanisms, Detection Methods, and Epidemiology

Paletta, Ana C. C / Castro, Vinicius S / Conte-Junior, Carlos A

Current microbiology. 2020 Apr., v. 77, no. 4

2020

Abstract: The main Enterobacteriaceae habitat is the mammal gastrointestinal tract. In most cases, this group of species displays a symbiotic relationship with its hosts. However, some groups may be pathogenic to humans, such as Shiga toxin-producing Escherichia ... ...

Abstract	The main Enterobacteriaceae habitat is the mammal gastrointestinal tract. In most cases, this group of species displays a symbiotic relationship with its hosts. However, some groups may be pathogenic to humans, such as Shiga toxin-producing Escherichia coli and enteroaggregative Escherichia coli. The presence of these groups represents a direct risk to consumers, and recent serotypes displaying the presence of pathogenic genes in both groups are a novel challenge for food production. Thus, microbiological control strategies presenting accurate detection methodologies are required. However, with the appearance of mutations among different species, knowledge, genetic monitoring, and bioinformatics techniques must be expanded. In addition, as a strategy to ensure safe products on an industrial scale, the monitoring by different techniques and fundamentals should be applied throughout the entire processing chain. Therefore, the aim of this review is to describe the pathogenesis mechanisms of different groups, mutant strain dispersion, and current and alternative epidemiological investigation methods.
Keywords	Shiga toxin-producing Escherichia coli ; bioinformatics ; digestive tract ; epidemiological studies ; food production ; habitats ; microbiology ; mutants ; pathogenesis ; pathogenicity ; risk ; serotypes ; symbiosis
Language	English
Dates of publication	2020-04
Size	p. 612-620.
Publishing place	Springer US
Document type	Article
Note	Review
ZDB-ID	134238-1
ISSN	1432-0991 ; 0343-8651
ISSN (online)	1432-0991
ISSN	0343-8651
DOI	10.1007/s00284-019-01842-1
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Shiga Toxin-Producing and Enteroaggregative Escherichia coli in Animal, Foods, and Humans: Pathogenicity Mechanisms, Detection Methods, and Epidemiology.

Paletta, Ana C C / Castro, Vinicius S / Conte-Junior, Carlos A

Current microbiology

2019 Volume 77, Issue 4, Page(s) 612–620

Abstract	The main Enterobacteriaceae habitat is the mammal gastrointestinal tract. In most cases, this group of species displays a symbiotic relationship with its hosts. However, some groups may be pathogenic to humans, such as Shiga toxin-producing Escherichia coli and enteroaggregative Escherichia coli. The presence of these groups represents a direct risk to consumers, and recent serotypes displaying the presence of pathogenic genes in both groups are a novel challenge for food production. Thus, microbiological control strategies presenting accurate detection methodologies are required. However, with the appearance of mutations among different species, knowledge, genetic monitoring, and bioinformatics techniques must be expanded. In addition, as a strategy to ensure safe products on an industrial scale, the monitoring by different techniques and fundamentals should be applied throughout the entire processing chain. Therefore, the aim of this review is to describe the pathogenesis mechanisms of different groups, mutant strain dispersion, and current and alternative epidemiological investigation methods.
MeSH term(s)	Animals ; Disease Outbreaks/prevention & control ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Escherichia coli/pathogenicity ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Food Safety/methods ; Foodborne Diseases/epidemiology ; Foodborne Diseases/microbiology ; Humans ; Mutation ; Phylogeny ; Shiga-Toxigenic Escherichia coli/genetics ; Shiga-Toxigenic Escherichia coli/isolation & purification ; Shiga-Toxigenic Escherichia coli/pathogenicity ; Virulence
Language	English
Publishing date	2019-12-13
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	134238-1
ISSN	1432-0991 ; 0343-8651
ISSN (online)	1432-0991
ISSN	0343-8651
DOI	10.1007/s00284-019-01842-1
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Article ; Online: Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro.

Teixeira, Izabel Mello / de Moraes Assumpção, Yasmim / Paletta, Ana Clara Cabral / Aguiar, Louise / Guimarães, Luciana / da Silva, Isabella Thomaz / Côrtes, Marina F / Botelho, Ana Maria Nunes / Jaeger, Lauren Hubert / Ferreira, Renata Fernandes / de Oliveira Ferreira, Eliane / Penna, Bruno

Scientific reports

2023 Volume 13, Issue 1, Page(s) 20219

Abstract: Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better ... ...

Abstract	Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population, we search the presence of MRSP in colonized or infected dogs. Samples from 99 dogs with infections and 35 from asymptomatic dogs were collected. Isolates were identified by mass spectrometry and Multiplex-PCR. The mecA gene was confirmed by conventional PCR. MRSP strains were analyzed by whole-genome sequencing. 75 S. pseudintermedius were identified, most from infection cases. The species were isolated from 70 out of the 135 dogs. Penicillin and Trimethoprim/Sulfamethoxazole presented higher resistance rates. Forty-seven strains were classified as multi-drug resistant (MDR), and were more isolated from dogs with infection (P < 0.05). Eighteen samples were classified as MRSP, representing 24.0% of the population. Six of 16 MRSP sequenced samples belonged to the world spread clone ST71; others belonged to unknown clones. Most samples carried the SCCmec type IIIA. Twenty-one different genetic resistance determinants were found among MRPS strains. MRSP is circulating among infected and colonized dogs in Rio de Janeiro, Brazil.
MeSH term(s)	Dogs ; Animals ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/veterinary ; Staphylococcal Infections/epidemiology ; Anti-Bacterial Agents/pharmacology ; Methicillin Resistance ; Brazil ; Dog Diseases/drug therapy ; Dog Diseases/epidemiology ; Multiplex Polymerase Chain Reaction ; Genetic Variation ; Microbial Sensitivity Tests
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-11-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-47549-z
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Article ; Online: Dengue virus NS5 degrades ERC1 during infection to antagonize NF-kB activation.

Gonzalez Lopez Ledesma, María Mora / Costa Navarro, Guadalupe / Pallares, Horacio M / Paletta, Ana / De Maio, Federico / Iglesias, Nestor G / Gebhard, Leopoldo / Oviedo Rouco, Santiago / Ojeda, Diego S / de Borba, Luana / Giraldo, María / Rajsbaum, Ricardo / Ceballos, Ana / Krogan, Nevan J / Shah, Priya S / Gamarnik, Andrea V

Proceedings of the National Academy of Sciences of the United States of America

2023 Volume 120, Issue 23, Page(s) e2220005120

Abstract: Dengue virus (DENV) is the most important human virus transmitted by mosquitos. Dengue pathogenesis is characterized by a large induction of proinflammatory cytokines. This cytokine induction varies among the four DENV serotypes (DENV1 to 4) and poses a ... ...

Abstract	Dengue virus (DENV) is the most important human virus transmitted by mosquitos. Dengue pathogenesis is characterized by a large induction of proinflammatory cytokines. This cytokine induction varies among the four DENV serotypes (DENV1 to 4) and poses a challenge for live DENV vaccine design. Here, we identify a viral mechanism to limit NF-κB activation and cytokine secretion by the DENV protein NS5. Using proteomics, we found that NS5 binds and degrades the host protein ERC1 to antagonize NF-κB activation, limit proinflammatory cytokine secretion, and reduce cell migration. We found that ERC1 degradation involves unique properties of the methyltransferase domain of NS5 that are not conserved among the four DENV serotypes. By obtaining chimeric DENV2 and DENV4 viruses, we map the residues in NS5 for ERC1 degradation, and generate recombinant DENVs exchanging serotype properties by single amino acid substitutions. This work uncovers a function of the viral protein NS5 to limit cytokine production, critical to dengue pathogenesis. Importantly, the information provided about the serotype-specific mechanism for counteracting the antiviral response can be applied to improve live attenuated vaccines.
MeSH term(s)	Humans ; Cytokines ; Dengue ; Dengue Virus ; NF-kappa B/metabolism ; Serogroup ; Viral Nonstructural Proteins/metabolism
Chemical Substances	Cytokines ; NF-kappa B ; NS5 protein, dengue virus ; Viral Nonstructural Proteins ; ERC1 protein, human
Language	English
Publishing date	2023-05-30
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2220005120
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Article ; Online: Classifying Nearest-Neighbor Interactions and Deformations of AdS.

de Leeuw, Marius / Paletta, Chiara / Pribytok, Anton / Retore, Ana L / Ryan, Paul

Physical review letters

2020 Volume 125, Issue 3, Page(s) 31604

Abstract: We classify all regular solutions of the Yang-Baxter equation of eight-vertex type. Regular solutions correspond to spin chains with nearest-neighbor interactions. We find a total of four independent solutions. Two are related to the usual six- and eight- ...

Abstract	We classify all regular solutions of the Yang-Baxter equation of eight-vertex type. Regular solutions correspond to spin chains with nearest-neighbor interactions. We find a total of four independent solutions. Two are related to the usual six- and eight-vertex models that have R matrices of difference form. We find two new solutions of the Yang-Baxter equation, which are manifestly of nondifference form. These new solutions contain the S-matrices of the AdS_{2} and AdS_{3} integrable models as a special case. This can be used as a starting point to study and classify integrable deformations of these holographic integrable systems.
Language	English
Publishing date	2020-08-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.125.031604
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Article ; Online: Resistance to Prostaglandin E2 Promotes Monocyte Activation During Chronic HIV Infection.

Di Diego Garcia, Facundo / Cabrerizo, Gonzalo / Paletta, Ana / Prez, Paula S / Varese, Augusto / Geffner, Jorge / Bello, Natalia / Fridman, Vanesa / Stecher, Daniel / Ceballos, Ana / Remes Lenicov, Federico

The Journal of infectious diseases

2022 Volume 227, Issue 3, Page(s) 423–433

Abstract: Background: Monocyte activation is a driver of inflammation in the course of chronic HIV infection. Prostaglandin E2 (PGE2) is known to mediate anti-inflammatory effects, notably the inhibition of tumor necrosis factor- (TNF-) production by monocytes. ... ...

Abstract	Background: Monocyte activation is a driver of inflammation in the course of chronic HIV infection. Prostaglandin E2 (PGE2) is known to mediate anti-inflammatory effects, notably the inhibition of tumor necrosis factor- (TNF-) production by monocytes. We aim to investigate the effects of PGE2 on activation of monocytes in chronic HIV infection and the mechanisms through which PGE2 modulates their inflammatory signature. Methods: We recruited a group of people with HIV (PWH) and matched healthy uninfected persons. We compared plasma levels of PGE2, monocyte activation, and sensitivity of monocytes to the inhibitory actions mediated by PGE2. Results: We found increased plasma levels of PGE2 in PWH, and an activated phenotype in circulating monocytes, compared with uninfected individuals. Monocytes from PWH showed a significant resistance to the inhibitory actions mediated by PGE2; the concentration of PGE2 able to inhibit 50 of the production of TNF- by lipopolysaccharide-stimulated monocytes was 10 times higher in PWH compared with uninfected controls. Furthermore, the expression of phosphodiesterase 4B, a negative regulator of PGE2 activity, was significantly increased in monocytes from PWH. Conclusions: Resistance to the inhibitory actions mediated by PGE2 could account, at least in part, for the inflammatory profile of circulating monocytes in PWH.
MeSH term(s)	Humans ; Dinoprostone/metabolism ; Monocytes/metabolism ; HIV Infections/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Gene Expression ; Lipopolysaccharides
Chemical Substances	Dinoprostone (K7Q1JQR04M) ; Tumor Necrosis Factor-alpha ; Lipopolysaccharides
Language	English
Publishing date	2022-12-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3019-3
ISSN	1537-6613 ; 0022-1899
ISSN (online)	1537-6613
ISSN	0022-1899
DOI	10.1093/infdis/jiac480
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Article ; Online: Platelets modulate CD4

Paletta, Ana / Di Diego García, Facundo / Varese, Augusto / Erra Diaz, Fernando / García, Julián / Cisneros, Juan Carlos / Ludueña, Guillermina / Mazzitelli, Ignacio / Pisarevsky, Andrea / Cabrerizo, Gonzalo / López Malizia, Álvaro / Rodriguez, Alejandra G / Lista, Nicolás / Longueira, Yesica / Sabatté, Juan / Geffner, Jorge / Remes Lenicov, Federico / Ceballos, Ana

British journal of haematology

2022 Volume 197, Issue 3, Page(s) 283–292

Abstract: Severe COVID-19 is associated with a systemic inflammatory response and progressive ... ...

Abstract	Severe COVID-19 is associated with a systemic inflammatory response and progressive CD4
MeSH term(s)	B7-H1 Antigen/metabolism ; Blood Platelets/metabolism ; CD4-Positive T-Lymphocytes ; COVID-19 ; Humans ; Interferon-gamma
Chemical Substances	B7-H1 Antigen ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2022-02-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.18062
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Article ; Online: AHR signaling is induced by infection with coronaviruses.

Giovannoni, Federico / Li, Zhaorong / Remes-Lenicov, Federico / Dávola, María E / Elizalde, Mercedes / Paletta, Ana / Ashkar, Ali A / Mossman, Karen L / Dugour, Andrea V / Figueroa, Juan M / Barquero, Andrea A / Ceballos, Ana / Garcia, Cybele C / Quintana, Francisco J

Nature communications

2021 Volume 12, Issue 1, Page(s) 5148

Abstract: Coronavirus infection in humans is usually associated to respiratory tract illnesses, ranging in severity from mild to life-threatening respiratory failure. The aryl hydrocarbon receptor (AHR) was recently identified as a host factor for Zika and dengue ... ...

Abstract	Coronavirus infection in humans is usually associated to respiratory tract illnesses, ranging in severity from mild to life-threatening respiratory failure. The aryl hydrocarbon receptor (AHR) was recently identified as a host factor for Zika and dengue viruses; AHR antagonists boost antiviral immunity, decrease viral titers and ameliorate Zika-induced pathology in vivo. Here we report that AHR is activated by infection with different coronaviruses, potentially impacting antiviral immunity and lung epithelial cells. Indeed, the analysis of single-cell RNA-seq from lung tissue detected increased expression of AHR and AHR transcriptional targets, suggesting AHR signaling activation in SARS-CoV-2-infected epithelial cells from COVID-19 patients. Moreover, we detected an association between AHR expression and viral load in SARS-CoV-2 infected patients. Finally, we found that the pharmacological inhibition of AHR suppressed the replication in vitro of one of the causative agents of the common cold, HCoV-229E, and the causative agent of the COVID-19 pandemic, SARS-CoV-2. Taken together, these findings suggest that AHR activation is a common strategy used by coronaviruses to evade antiviral immunity and promote viral replication, which may also contribute to lung pathology. Future studies should further evaluate the potential of AHR as a target for host-directed antiviral therapy.
MeSH term(s)	COVID-19/genetics ; COVID-19/metabolism ; COVID-19/virology ; Coronavirus/physiology ; Coronavirus Infections/genetics ; Coronavirus Infections/metabolism ; Coronavirus Infections/virology ; Epithelial Cells/metabolism ; Epithelial Cells/virology ; Female ; Humans ; Male ; Receptors, Aryl Hydrocarbon/genetics ; Receptors, Aryl Hydrocarbon/metabolism ; SARS-CoV-2/physiology ; Signal Transduction
Chemical Substances	Receptors, Aryl Hydrocarbon
Language	English
Publishing date	2021-08-26
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-021-25412-x
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Article ; Online: Immunoglobulin G Immune Complexes May Contribute to Neutrophil Activation in the Course of Severe Coronavirus Disease 2019.

Mazzitelli, Ignacio / Bleichmar, Lucia / Ludueña, María Guillermina / Pisarevsky, Andrea / Labato, Mariana / Chiaradia, Verónica / Finocchieto, Paola / Paulin, Francisco / Hormanstorfer, Macarena / Baretto, María Constanza / Adanza, Santiago Piombi / Parodi, María Noel / Ragusa, Martín / Melucci, Claudia / Díaz, Fernando Erra / Paletta, Ana / Di Diego, Facundo / Ceballos, Ana / Geffner, Jorge

The Journal of infectious diseases

2021 Volume 224, Issue 4, Page(s) 575–585

Abstract: Severe coronavirus disease 2019 (COVID-19) is associated with an overactive inflammatory response mediated by macrophages. Here, we analyzed the phenotype and function of neutrophils in patients with COVID-19. We found that neutrophils from patients with ...

Abstract	Severe coronavirus disease 2019 (COVID-19) is associated with an overactive inflammatory response mediated by macrophages. Here, we analyzed the phenotype and function of neutrophils in patients with COVID-19. We found that neutrophils from patients with severe COVID-19 express high levels of CD11b and CD66b, spontaneously produce CXCL8 and CCL2, and show a strong association with platelets. Production of CXCL8 correlated with plasma concentrations of lactate dehydrogenase and D-dimer. Whole blood assays revealed that neutrophils from patients with severe COVID-19 show a clear association with immunoglobulin G (IgG) immune complexes. Moreover, we found that sera from patients with severe disease contain high levels of immune complexes and activate neutrophils through a mechanism partially dependent on FcγRII (CD32). Interestingly, when integrated in immune complexes, anti-severe acute respiratory syndrome coronavirus 2 IgG antibodies from patients with severe COVID-19 displayed a higher proinflammatory profile compared with antibodies from patients with mild disease. Our study suggests that IgG immune complexes might promote the acquisition of an inflammatory signature by neutrophils, worsening the course of COVID-19.
MeSH term(s)	Adult ; Aged ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Antigen-Antibody Complex/blood ; Antigen-Antibody Complex/immunology ; Antigens, CD/immunology ; CD11b Antigen/immunology ; COVID-19/immunology ; Cell Adhesion Molecules/immunology ; Female ; GPI-Linked Proteins/immunology ; Humans ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Interleukin-8/immunology ; Male ; Middle Aged ; Neutrophil Activation/immunology ; Neutrophils/immunology ; Receptors, IgG/immunology ; SARS-CoV-2/immunology ; Young Adult
Chemical Substances	Antibodies, Viral ; Antigen-Antibody Complex ; Antigens, CD ; CD11b Antigen ; CEACAM8 protein, human ; CXCL8 protein, human ; Cell Adhesion Molecules ; GPI-Linked Proteins ; Immunoglobulin G ; Interleukin-8 ; Receptors, IgG
Language	English
Publishing date	2021-08-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3019-3
ISSN	1537-6613 ; 0022-1899
ISSN (online)	1537-6613
ISSN	0022-1899
DOI	10.1093/infdis/jiab174
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Article ; Online: Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for accurate discrimination of Staphylococcus schleiferi subspecies.

Assumpção, Yasmim de Moraes / Teixeira, Izabel M / Paletta, Ana Clara Cabral / Ferreira, Eliane de Oliveira / Pinto, Tatiana C A / Penna, Bruno de A

Veterinary microbiology

2019 Volume 240, Page(s) 108472

Abstract: S. schleiferi is one of the main species isolated from canine otitis externa, pyoderma and from apparently healthy dogs. The species is divided into two subspecies, S. schleiferi schleiferi and S. schleiferi coagulans. MALDI-TOF MS does not distinguish ... ...

Abstract	S. schleiferi is one of the main species isolated from canine otitis externa, pyoderma and from apparently healthy dogs. The species is divided into two subspecies, S. schleiferi schleiferi and S. schleiferi coagulans. MALDI-TOF MS does not distinguish correctly these two subspecies. This study aimed to identify biomarkers that could possibly discriminate Staphylococcus schleiferi subspecies by MALDI-TOF MS. Twelve strains (eight S. schleiferi schleiferi and four S. schleiferi coagulans) were firstly identified. Each isolate was submitted to a protein extraction protocol and subjected to spectrometry on Bruker Microflex LT mass spectrometer. Spectra were analyzed with the BioNumerics software v7.6. Our results showed that spectra clustered according to subspecies, and a set of five MALDI-TOF MS biomarkers were selected to enable the discrimination of S. schleiferi subspecies. In addition, these biomarkers were predicted to represent highly conserved proteins, which could contribute to the identification of subspecies-specific proteins that could be used for improved subspecies identification in clinical samples.
MeSH term(s)	Animals ; Biomarkers/analysis ; Dogs ; Prospective Studies ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Staphylococcus/classification ; Staphylococcus/isolation & purification
Chemical Substances	Biomarkers
Language	English
Publishing date	2019-10-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	753154-0
ISSN	1873-2542 ; 0378-1135
ISSN (online)	1873-2542
ISSN	0378-1135
DOI	10.1016/j.vetmic.2019.108472
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